scholarly journals Microbiological Analysis of Different Flavoured Milk Samples Collected from Various Areas of Dhaka city, Bangladesh

2018 ◽  
Vol 33 (1-2) ◽  
pp. 11-15
Author(s):  
Shirin Tabassum ◽  
Md Aftab Uddin
Keyword(s):  
Microbial Contamination ◽  
Microbiological Analysis ◽  
Drug Resistant ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Milk Samples ◽  
Antibiotic Susceptibility Test ◽  
The Common ◽  
Staphylococcus Spp

Current study attempted to examine the chances of microbial contamination within the common flavored milk products consumed by the locality of Dhaka city, Bangladesh. All samples, collected from different super shops, revealed the presence of total viable bacterial counts within the range of 2.5×104 to 8×105 cfu/ mL. Staphylococcus spp., Pseudomonas spp., and coliforms were noticed to be predominant and were recovered from 6 out of 10 samples with an average load of 103-105 cfu/mL. Salmonella spp. was also detected in one sample. Study of the antibiotic susceptibility test further demonstrated that all the bacterial isolates were resistant against most of the commonly used antibiotics with the multi- drug resistant traits in several cases. Bangladesh J Microbiol, Volume 33, Number 1-2, June-Dec 2016, pp 11-15

scholarly journals Microbiological analysis and determination of antibacterial activity of apple samples collected from local markets in Dhaka city, Bangladesh

2017 ◽  
Vol 6 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Sheikh Al Mamun ◽  
Kamal Kanta Das ◽  
Md Aftab Uddin
Keyword(s):  
Antibacterial Activity ◽  
Local Market ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Viable Bacteria ◽  
Staphylococcus Spp

Present study attempted to detect the prevalence of contaminating microorganisms in 10 categories of local market apple samples commonly consumed in Dhaka city, Bangladesh. A significant number of total viable bacteria were quantified up to 106 cfu/g in these samples. Staphylococcus spp. was the predominant isolate within a range of 1.0×104– 3.2×106cfu/g whereas Escherichia coli, Klebsiella spp., Salmonella spp., Shigella spp., Pseudomonas spp. and Vibrio spp. were also quantified. Fungal contamination (within the range of 105 cfu/g – 107cfu/g) was also observed. Alarmingly most of the bacterial isolates present in the samples were proved to be multi-drug resistant. Antibacterial activity of the samples was tested against some common bacterial isolates but was found to be ineffective.Stamford Journal of Microbiology, Vol.6(1) 2016: 11-15

scholarly journals Detection of microbial proliferation & assessment of antibacterial activity of spices collected from various locations of Dhaka city, Bangladesh

2017 ◽  
Vol 7 (1) ◽  
pp. 10-13
Author(s):  
Md Aftab Uddin ◽  
Khondakar Wahid Hasan ◽  
Jubaida Binta Jamal
Keyword(s):  
Antibacterial Activity ◽  
Zingiber Officinale ◽  
Bacterial Count ◽  
Syzygium Aromaticum ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Two Samples ◽  
Microbial Proliferation ◽  
Staphylococcus Spp

The current study was aimed to detect the presence of contaminating microorganisms in some commonly available spices collected from different areas of Dhaka city, Bangladesh and also to assess their antibacterial activity. Out of eight samples under this study, total viable bacterial count (TVBC) was observed within the range of 106 to 107cfu/g. Among the specific bacterial isolates Staphylococcus spp., Pseudomonas spp. and Escherichia coli were found in almost all the samples within the margin of 102 to 106 cfu/g. Alarmingly Salmonella spp. and Vibrio spp. were presumptively identified in one and two samples respectively. Besides every sample showed the presence of fungus with load up to 103 cfu/g. Antibacterial activity of these samples was tested against seven laboratory isolates. Syzygium aromaticum showed highest activity against as many as five bacterial isolates while Cinnamomum verum was active against four isolates. On the other contrary, both Zingiber officinale and Laurus nobilis demonstrated their antibacterial activity only against Staphylococcus spp. and Klebsiella spp. respectively. Stamford Journal of Microbiology, Vol.7(1) 2017: 10-13

scholarly journals Microbiological analysis of raw milk, pasteurized milk and yogurt samples collected from different areas of Dhaka city, Bangladesh

2015 ◽  
Vol 39 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Tasmina Rahman ◽  
Tanzia Akon ◽  
Iftikharun Nessa Sheuli ◽  
Naima Hoque
Keyword(s):  
Listeria Monocytogenes ◽  
Bacterial Count ◽  
Raw Milk ◽  
Total Coliform ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Shigella Spp

A total of 300 different milk and yogurt samples were collected from Dhaka city and analyzed for total viable bacterial count (TVBC), total coliform count (TCC) and presence of several pathogens such as Escherichia coli, Salmonella spp., Shigella spp., Vibrio spp. and Listeria monocytogenes. Raw milk samples collected from Moghbazar area showed highest TVBC (4.2 × 106 cfu/ml) and samples from Mohammadpur area showed lowest count (3.5 × 103 cfu/ml). All the pasteurized milk samples showed TVBC of 1.9 × 102 to 2.8 × 103 cfu/ml, and TCC only in one sample Yogurt samples (open) collected from vendor showed TVBC of 9.1 × 103 to 8.2 × 107 cfu/ml, and TCC only in sample collected from Dhanmondi, Dhaka. The packed yogurt samples showed TVBC of 2.2 × 102 to 6.1 × 103 cfu/ml, and TCC only in one sample. All the milk and yogurt samples were found contaminated with E. coli and Shigella-like species. Listeria monocytogenes was not detected in any of the samples studied.Journal of Bangladesh Academy of Sciences, Vol. 39, No. 1, 31-36, 2015

scholarly journals Microbiological analysis and sensitivity profile of Staphylococcus spp. in subclinical mastitis of dairy goats

2016 ◽  
Vol 68 (2) ◽  
pp. 336-344
Author(s):  
S.R.S. Salaberry ◽  
A.B.S. Saidenberg ◽  
E. Zuniga ◽  
F.F. Gonsales ◽  
P.A. Melville ◽  
...  
Keyword(s):  
Subclinical Mastitis ◽  
Economic Losses ◽  
Microbiological Analysis ◽  
Dairy Goats ◽  
Milk Samples ◽  
California Mastitis Test ◽  
Resistant Strains ◽  
Staphylococcus Spp ◽  
Sensitivity Profile

Subclinical mastitis in goats causes economic losses and risks to public health. Given the need for research that shows the most isolated staphylococci species and sensibility tests comparing the resistance between coagulase-negative (CNS) and positive Staphylococcus (CPS) goats with subclinical mastitis, the aim of this study was to identify the microorganisms isolated from milk samples of goats with subclinical mastitis, as well as define the staphylococci species and determine the sensitivity profile of Staphylococcus spp. to antimicrobials. To collect samples, tests were performed for mug of black background and California mastitis test (CMT), collecting milk from CMT positive animals. A total of 226 samples from seven herds of dairy goats was collected and forwarded to the laboratory, where they were seeded for the isolation of the microorganism and implementing the antibiotic sensibility test. Of these, 122 samples had bacterial growth and the most isolated staphylococci species were: S. epidermidis (24.55%), S. lugdunensis (15.40%) and S. intermedius (13.64%). Samples showed increased resistance to antimicrobials: penicillin (81.8%), oxacillin (60.0%) and ampicillin (55.5%). Greater sensitivity to: enrofloxacin (99.1%), erythromycin (98.2%), gentamicin (98.2%) and vancomycin (98.2%) were observed. The S. epidermidis showed higher antimicrobial resistance to amoxicillin and penicillin than S. lugdunensis and S. intermedius. Similar resistance in vitro between CNS and CPS was observed to most antimicrobials. It is important to control the overuse of antibiotics to prevent the emergence of resistant strains.

scholarly journals Isolation and quantification of indicator and pathogenic microorganisms along with their drug resistance traits from bottled and jar water samples within Dhaka city, Bangladesh

2020 ◽  
Vol 9 (1) ◽  
pp. 12-14
Author(s):  
Md Aftab Uddin ◽  
Mst Aysha Siddiqua ◽  
Mst Sadia Ahmed
Keyword(s):  
Water Samples ◽  
Bacterial Count ◽  
Fecal Coliforms ◽  
Pathogenic Microorganisms ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Potential Threat ◽  
Antibiotic Susceptibility Pattern ◽  
Shigella Spp

Commercial drinking water may serve as potential threat to public health if these items are contaminated with a number of pathogenic microorganisms due to faulty manufacturing process. Present study attempted to isolate and quantify the microorganisms from various jar and bottle water samples collected from various areas of Dhaka city. Antibiotic susceptibility pattern of suspected bacterial isolates were also determined in this study. Out of the eighteen samples studied, ten were jar water samples and eight were bottled water samples. The range of total viable bacterial count (TVBC) in these samples ranged from 102 to 105 cfu/ml. Specific pathogens such as, Salmonella spp., Shigella spp., Vibrio spp. and fecal coliforms could not be found in these samples. However coliforms could be detected in 10 samples. The antibiogram study showed that all Escherichia coli and Klebsiella spp. isolates found from these samples were sensitive against gentamicin (10 μg) and azithromycin (30 μg). Variable antibiotic resistance among these bacterial isolates was detected against cefotaxime (30 μg), streptomycin (10 μg) and erythromycin (15 μg). Stamford Journal of Microbiology, Vol.9(1) 2019: 12-14

scholarly journals Microbiological analysis of different categories of food items in Dhaka city, Bangladesh

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1351-1356
Author(s):  
M.A. Uddin ◽  
T. Jabin ◽  
E.A. Siam ◽  
M.T. Afreen ◽  
S.S. Uddin ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Food Samples ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Wide Range ◽  
Shigella Spp

Pathogenic microorganisms have been so far reported to contaminate a wide range of foods triggering food borne infections or intoxications including the enteric complications, abdominal pain, fever, bloodstream infection etc. The current study was attempted to observe the microbiological quality of some popular foods collected from different places of Dhaka city, Bangladesh. Conventional cultural, microscopic and biochemical tests were followed for the detection and enumeration of bacterial isolates associated with these food samples. The investigation encompassed detection of total viable bacterial count (TVBC) and presumptive identification of other pathogenic bacteria from these samples. Higher counts of TVBC, coliform, Staphylococcus spp. and fungal load were recorded as 1.46×107 CFU/g (yogurt 1), 4.5×106 CFU/g (yogurt 1), 5.6×105 CFU/g (raw meat) and 2.9×103 CFU/g (sea fish), respectively. Fecal coliform was detected only in one out of ten samples. On the contrary Salmonella spp., Vibrio spp., Shigella spp. and Pseudomonas spp. were completely absent. The antibiogram study showed that all the isolates were sensitive against Kanamycin and Azithromycin. Better sensitivity was also observed against Gentamycin and Ciprofloxacin. Varying degree of antibiotic resistance was also detected against Cefixime, Amikacin and Neomycin. Our study emphasizes the need for continuous monitoring of the various categories of food samples for the safety of public health.

scholarly journals Detection of Potential Bacterial Pathogens and Aflatoxigenic Fungi from Grain Samples

2019 ◽  
Vol 7 (5) ◽  
pp. 731
Author(s):  
Hinda Abdukadir Mohamed ◽  
Md. Salauddin ◽  
Md. Khaled Hossain ◽  
Farzana Afroz
Keyword(s):  
Preventive Measures ◽  
Research Work ◽  
Bacterial Pathogen ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Aflatoxigenic Fungi ◽  
Identification Techniques ◽  
Staphylococcus Spp ◽  
Effective Drugs ◽  
Klebsiella Spp

Current research work was carried out for the detection of potential bacterial pathogen and aflatoxigenic fungi Aspergillus spp. from grain comprising [Rice (5), Maize (5), Wheat (5), Khessari dal (5) and Anchora dal (5)] were collected from 3 different local markets of Dinajpur District, Bangladesh. 15 bacterial isolates comprising 4 genera of bacteria were found from a total of 25 samples. The isolated bacteria were Staphylococcus spp., Escherichia coli, Klebsiella spp., Salmonella spp. with 16%, 28%, 16% and 16% prevalence respectively. Antibiogram studies revealed that overall effective drugs against isolated bacteria were Ciprofloxacin followed by Gentamycin. But resistant drugs were Penicillin, Vancomycin, Erythromycin, Kanamycin, and Amoxicillin. The variation in the sensitivity of common antibiotic could be the result of extensive and indiscriminate use of these antibiotics. Aspergillus spp. was isolated from 4-grain samples with 16% prevalence. But aflatoxigenic Aspergillus spp. was isolated from 3 samples with 12% prevalence. From the wheat samples and maize, the aflatoxigenic fungus was isolated and their prevalence in maize, wheat was 40% and 20% respectively. Their early detection can help to take preventive measures to combat economic and health losses. The study showed that earlier detections can be made by simple traditional identifications using macro and micromorphological fungal features rather than adopting the time and cost consuming molecular identification techniques.

scholarly journals Occurrence of pathogenic microorganisms in dessert items collected from Dhaka city

2020 ◽  
Vol 9 (1) ◽  
pp. 19-22
Author(s):  
Shohana Akter ◽  
Anasua Sarkar ◽  
Kamal Kanta Das
Keyword(s):  
Heterotrophic Bacteria ◽  
Good Health ◽  
Poor Quality ◽  
Dhaka City ◽  
Salmonella Spp ◽  
E Coli ◽  
Shigella Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Due to delicious taste and readily availability, desserts are one of the most popular foods in Dhaka city. High amount of carbohydrate and protein in dessert items make them more susceptible to proliferation of microbial growth. Present study depicted a complete microbiological profile of some popular desserts such as, sweet, pastry, ice cream, pudding, falooda, yogurt and custard available in different food shop at Dhaka city, Bangladesh. All the samples were found to be contaminated with heterotrophic bacteria as well as fungi within the range of 103 to 106 cfu/g. In case of specific microflora, the growth of Staphylococcus spp., Klebsiella spp. and Pseudomonas spp. were observed in most of the samples indicates the poor quality of these products. Bioburdens of E. coli in sweet, pudding and yogurt were found in the range of 1.2×102 to 2.7×103 cfu/g. Salmonella spp., Shigella spp. and Vibrio spp. could not be isolated from any of the samples. Current study indicates that hygienic conditions should be maintained during preparation, packaging and retailing of dessert items in order to reduce the load of contamination in ready to eat foods which will ensure good health of consumers. Stamford Journal of Microbiology, Vol.9(1) 2019: 19-22

scholarly journals ISOLATION, IDENTIFICATION AND ANTIBIOTIC RESISTANCE OF AEROMONAS SPP. AND SALMONELLA SPP. FROM THE FRESH WATER LOACH, LEPIDOCEPHALICHTHYS GUNTEA AND WATER OF TERAI RIVER LOTCHKA, WEST BENGAL, INDIA

2013 ◽  
Vol 58 (1-2) ◽  
pp. 5-17 ◽  
Author(s):  
Rudra Prasad Roy ◽  
Min Bahadur ◽  
Sudip Barat
Keyword(s):  
Antibiotic Resistance ◽  
Fresh Water ◽  
Pathogenic Bacteria ◽  
West Bengal ◽  
Penicillin G ◽  
Diffusion Method ◽  
Body Parts ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Antibiotic Susceptibility Test

Abstract An investigation was conducted to identify the bacterial isolates and to study the antibiotic resistance patterns of Aeromonas spp. and Salmonella spp. from the skin, gills, and gut of the fresh water loach, Lepidocephalichthys guntea (HAMILTON BUCHANAN) and water sampled from four different sites along the River Lotchka in Darjeeling District, West Bengal, India. Isolated bacteria were identified by different biochemical procedures, and Polymerase Chain Reaction was performed using genus specific 16S rDNA primers for confirmation of identification of Aeromonas spp. and Salmonella spp. Antibiotic susceptibility test of bacterial isolates was also done by Disc Diffusion method. A total of 49 Aeromonas spp. and 24 Salmonella spp. were isolated from tested samples. Maximum resistance was exhibited for Penicillin-G, Ampicillin, and Cephalothin (>75% resistant Aeromonas spp. and >60% Salmonella spp. in the four different sites of river and body parts of fish). Ciprofloxacin and Tetracycline resistance was almost nil. The results indicated that the river water and resident fish were contaminated with multi-antibiotic resistant enteric pathogenic bacteria. This study, thus, provides valuable information for making policy decisions aimed at reducing microbial contamination of fish and the indiscriminate use of antibiotics.

scholarly journals Evaluation of microbial contamination level and the drug susceptible pattern of the isolates cultivated from famous dessert food

Food Research ◽  
2021 ◽  
Vol 5 (1) ◽  
pp. 426-430
Author(s):  
T. Akter ◽  
T. Ishma ◽  
S.R. Razzeb ◽  
S.H.M. Uddin ◽  
M. Islam ◽  
...  
Keyword(s):  
Microbial Contamination ◽  
Heterotrophic Bacteria ◽  
Ice Cream ◽  
Drug Susceptibility ◽  
Diffusion Method ◽  
Contamination Level ◽  
Salmonella Spp ◽  
E Coli ◽  
Bacillus Spp ◽  
Staphylococcus Spp

Present study endeavored to evaluate the microbial contamination level along with their drug resistant pattern in some popular desert food items collected from different food shops in Dhaka city, Bangladesh. The microbial evaluation was conducted through conventional cultural methods and drug susceptibility test was executed through disc diffusion method. All the samples were found to be contaminated with heterotrophic bacteria as well as fungi within the range of 103 to 105 CFU/g. In case of specific microflora, the growth of Staphylococcus spp. was very high in sweet, faluda, milk cake and ice cream as estimated up to 105 CFU/g, halua and sweet yogurt showed 104 CFU/g while rest of the samples revealed 103 CFU/g. E. coli was found only in faluda and ice cream up to 103 CFU/g whereas Klebsiella spp. was estimated in all the samples within the range of 102 CFU/g to 105 CFU/g. Salmonella spp., Pseudomonas spp. and Bacillus spp. were totally absent in all the samples. Most of the isolates were found to be resistant against most of the antibiotics. Meanwhile, streptomycin (10 µg), gentamicin (10 µg), azithromycin (15 µg), and nalidixic acid (5 µg) were effective drug against both E. coli and Staphylococcus spp.

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