scholarly journals Microbiological analysis and determination of antibacterial activity of apple samples collected from local markets in Dhaka city, Bangladesh

2017 ◽  
Vol 6 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Sheikh Al Mamun ◽  
Kamal Kanta Das ◽  
Md Aftab Uddin
Keyword(s):  
Antibacterial Activity ◽  
Local Market ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Shigella Spp ◽  
Viable Bacteria ◽  
Staphylococcus Spp

Present study attempted to detect the prevalence of contaminating microorganisms in 10 categories of local market apple samples commonly consumed in Dhaka city, Bangladesh. A significant number of total viable bacteria were quantified up to 106 cfu/g in these samples. Staphylococcus spp. was the predominant isolate within a range of 1.0×104– 3.2×106cfu/g whereas Escherichia coli, Klebsiella spp., Salmonella spp., Shigella spp., Pseudomonas spp. and Vibrio spp. were also quantified. Fungal contamination (within the range of 105 cfu/g – 107cfu/g) was also observed. Alarmingly most of the bacterial isolates present in the samples were proved to be multi-drug resistant. Antibacterial activity of the samples was tested against some common bacterial isolates but was found to be ineffective.Stamford Journal of Microbiology, Vol.6(1) 2016: 11-15

scholarly journals Detection of microbial proliferation & assessment of antibacterial activity of spices collected from various locations of Dhaka city, Bangladesh

2017 ◽  
Vol 7 (1) ◽  
pp. 10-13
Author(s):  
Md Aftab Uddin ◽  
Khondakar Wahid Hasan ◽  
Jubaida Binta Jamal
Keyword(s):  
Antibacterial Activity ◽  
Zingiber Officinale ◽  
Bacterial Count ◽  
Syzygium Aromaticum ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Two Samples ◽  
Microbial Proliferation ◽  
Staphylococcus Spp

The current study was aimed to detect the presence of contaminating microorganisms in some commonly available spices collected from different areas of Dhaka city, Bangladesh and also to assess their antibacterial activity. Out of eight samples under this study, total viable bacterial count (TVBC) was observed within the range of 106 to 107cfu/g. Among the specific bacterial isolates Staphylococcus spp., Pseudomonas spp. and Escherichia coli were found in almost all the samples within the margin of 102 to 106 cfu/g. Alarmingly Salmonella spp. and Vibrio spp. were presumptively identified in one and two samples respectively. Besides every sample showed the presence of fungus with load up to 103 cfu/g. Antibacterial activity of these samples was tested against seven laboratory isolates. Syzygium aromaticum showed highest activity against as many as five bacterial isolates while Cinnamomum verum was active against four isolates. On the other contrary, both Zingiber officinale and Laurus nobilis demonstrated their antibacterial activity only against Staphylococcus spp. and Klebsiella spp. respectively. Stamford Journal of Microbiology, Vol.7(1) 2017: 10-13

scholarly journals Microbiological Analysis of Different Flavoured Milk Samples Collected from Various Areas of Dhaka city, Bangladesh

2018 ◽  
Vol 33 (1-2) ◽  
pp. 11-15
Author(s):  
Shirin Tabassum ◽  
Md Aftab Uddin
Keyword(s):  
Microbial Contamination ◽  
Microbiological Analysis ◽  
Drug Resistant ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Milk Samples ◽  
Antibiotic Susceptibility Test ◽  
The Common ◽  
Staphylococcus Spp

Current study attempted to examine the chances of microbial contamination within the common flavored milk products consumed by the locality of Dhaka city, Bangladesh. All samples, collected from different super shops, revealed the presence of total viable bacterial counts within the range of 2.5×104 to 8×105 cfu/ mL. Staphylococcus spp., Pseudomonas spp., and coliforms were noticed to be predominant and were recovered from 6 out of 10 samples with an average load of 103-105 cfu/mL. Salmonella spp. was also detected in one sample. Study of the antibiotic susceptibility test further demonstrated that all the bacterial isolates were resistant against most of the commonly used antibiotics with the multi- drug resistant traits in several cases. Bangladesh J Microbiol, Volume 33, Number 1-2, June-Dec 2016, pp 11-15

scholarly journals Isolation and quantification of indicator and pathogenic microorganisms along with their drug resistance traits from bottled and jar water samples within Dhaka city, Bangladesh

2020 ◽  
Vol 9 (1) ◽  
pp. 12-14
Author(s):  
Md Aftab Uddin ◽  
Mst Aysha Siddiqua ◽  
Mst Sadia Ahmed
Keyword(s):  
Water Samples ◽  
Bacterial Count ◽  
Fecal Coliforms ◽  
Pathogenic Microorganisms ◽  
Dhaka City ◽  
Bacterial Isolates ◽  
Salmonella Spp ◽  
Potential Threat ◽  
Antibiotic Susceptibility Pattern ◽  
Shigella Spp

Commercial drinking water may serve as potential threat to public health if these items are contaminated with a number of pathogenic microorganisms due to faulty manufacturing process. Present study attempted to isolate and quantify the microorganisms from various jar and bottle water samples collected from various areas of Dhaka city. Antibiotic susceptibility pattern of suspected bacterial isolates were also determined in this study. Out of the eighteen samples studied, ten were jar water samples and eight were bottled water samples. The range of total viable bacterial count (TVBC) in these samples ranged from 102 to 105 cfu/ml. Specific pathogens such as, Salmonella spp., Shigella spp., Vibrio spp. and fecal coliforms could not be found in these samples. However coliforms could be detected in 10 samples. The antibiogram study showed that all Escherichia coli and Klebsiella spp. isolates found from these samples were sensitive against gentamicin (10 μg) and azithromycin (30 μg). Variable antibiotic resistance among these bacterial isolates was detected against cefotaxime (30 μg), streptomycin (10 μg) and erythromycin (15 μg). Stamford Journal of Microbiology, Vol.9(1) 2019: 12-14

scholarly journals Microbiological analysis of different categories of food items in Dhaka city, Bangladesh

Food Research ◽  
2020 ◽  
Vol 4 (4) ◽  
pp. 1351-1356
Author(s):  
M.A. Uddin ◽  
T. Jabin ◽  
E.A. Siam ◽  
M.T. Afreen ◽  
S.S. Uddin ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Microbiological Quality ◽  
Bacterial Count ◽  
Food Samples ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Biochemical Tests ◽  
Salmonella Spp ◽  
Wide Range ◽  
Shigella Spp

Pathogenic microorganisms have been so far reported to contaminate a wide range of foods triggering food borne infections or intoxications including the enteric complications, abdominal pain, fever, bloodstream infection etc. The current study was attempted to observe the microbiological quality of some popular foods collected from different places of Dhaka city, Bangladesh. Conventional cultural, microscopic and biochemical tests were followed for the detection and enumeration of bacterial isolates associated with these food samples. The investigation encompassed detection of total viable bacterial count (TVBC) and presumptive identification of other pathogenic bacteria from these samples. Higher counts of TVBC, coliform, Staphylococcus spp. and fungal load were recorded as 1.46×107 CFU/g (yogurt 1), 4.5×106 CFU/g (yogurt 1), 5.6×105 CFU/g (raw meat) and 2.9×103 CFU/g (sea fish), respectively. Fecal coliform was detected only in one out of ten samples. On the contrary Salmonella spp., Vibrio spp., Shigella spp. and Pseudomonas spp. were completely absent. The antibiogram study showed that all the isolates were sensitive against Kanamycin and Azithromycin. Better sensitivity was also observed against Gentamycin and Ciprofloxacin. Varying degree of antibiotic resistance was also detected against Cefixime, Amikacin and Neomycin. Our study emphasizes the need for continuous monitoring of the various categories of food samples for the safety of public health.

scholarly journals Occurrence of pathogenic microorganisms in dessert items collected from Dhaka city

2020 ◽  
Vol 9 (1) ◽  
pp. 19-22
Author(s):  
Shohana Akter ◽  
Anasua Sarkar ◽  
Kamal Kanta Das
Keyword(s):  
Heterotrophic Bacteria ◽  
Good Health ◽  
Poor Quality ◽  
Dhaka City ◽  
Salmonella Spp ◽  
E Coli ◽  
Shigella Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

Due to delicious taste and readily availability, desserts are one of the most popular foods in Dhaka city. High amount of carbohydrate and protein in dessert items make them more susceptible to proliferation of microbial growth. Present study depicted a complete microbiological profile of some popular desserts such as, sweet, pastry, ice cream, pudding, falooda, yogurt and custard available in different food shop at Dhaka city, Bangladesh. All the samples were found to be contaminated with heterotrophic bacteria as well as fungi within the range of 103 to 106 cfu/g. In case of specific microflora, the growth of Staphylococcus spp., Klebsiella spp. and Pseudomonas spp. were observed in most of the samples indicates the poor quality of these products. Bioburdens of E. coli in sweet, pudding and yogurt were found in the range of 1.2×102 to 2.7×103 cfu/g. Salmonella spp., Shigella spp. and Vibrio spp. could not be isolated from any of the samples. Current study indicates that hygienic conditions should be maintained during preparation, packaging and retailing of dessert items in order to reduce the load of contamination in ready to eat foods which will ensure good health of consumers. Stamford Journal of Microbiology, Vol.9(1) 2019: 19-22

scholarly journals Microbiological analysis of raw milk, pasteurized milk and yogurt samples collected from different areas of Dhaka city, Bangladesh

2015 ◽  
Vol 39 (1) ◽  
pp. 31-36 ◽  
Author(s):  
Tasmina Rahman ◽  
Tanzia Akon ◽  
Iftikharun Nessa Sheuli ◽  
Naima Hoque
Keyword(s):  
Listeria Monocytogenes ◽  
Bacterial Count ◽  
Raw Milk ◽  
Total Coliform ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Pasteurized Milk ◽  
Milk Samples ◽  
Shigella Spp

A total of 300 different milk and yogurt samples were collected from Dhaka city and analyzed for total viable bacterial count (TVBC), total coliform count (TCC) and presence of several pathogens such as Escherichia coli, Salmonella spp., Shigella spp., Vibrio spp. and Listeria monocytogenes. Raw milk samples collected from Moghbazar area showed highest TVBC (4.2 × 106 cfu/ml) and samples from Mohammadpur area showed lowest count (3.5 × 103 cfu/ml). All the pasteurized milk samples showed TVBC of 1.9 × 102 to 2.8 × 103 cfu/ml, and TCC only in one sample Yogurt samples (open) collected from vendor showed TVBC of 9.1 × 103 to 8.2 × 107 cfu/ml, and TCC only in sample collected from Dhanmondi, Dhaka. The packed yogurt samples showed TVBC of 2.2 × 102 to 6.1 × 103 cfu/ml, and TCC only in one sample. All the milk and yogurt samples were found contaminated with E. coli and Shigella-like species. Listeria monocytogenes was not detected in any of the samples studied.Journal of Bangladesh Academy of Sciences, Vol. 39, No. 1, 31-36, 2015

scholarly journals Assessment of the Microbiological Quality of Ready-To Fry Frozen Chicken-Based Snack Items Sold in Bangladesh

2021 ◽  
pp. 52-56
Author(s):  
Anamika Chakrabarty ◽  
Tamanna Islam ◽  
Saurab Kishore Munshi
Keyword(s):  
Foodborne Pathogens ◽  
Microbiological Quality ◽  
Multidrug Resistant ◽  
Resistance Pattern ◽  
Microbiological Analysis ◽  
Chicken Nuggets ◽  
Salmonella Spp ◽  
Viable Bacteria ◽  
Bacteria And Fungi ◽  
Staphylococcus Spp

Microorganisms that are capable of spoiling the product during chill storage as well as several foodborne pathogens can be present in frozen snacks. The present study attempted to investigate the presence of microbial contaminants along with their antibiotic resistance pattern in frozen snacks. In this regard, a total of 15 ready-to-fry frozen chicken-based snack items including three each of chicken pops, chicken samosa, chicken lemongrass lollypop, chicken nuggets and chicken meatball were collected from super shops of Dhaka, Bangladesh. Microbiological analysis revealed that all the samples were highly contaminated with total viable bacteria and fungi in an average of 6 and 4 log cfu/g, respectively which exceeded the standard microbial limit. Klebsiella spp., Staphylococcus spp. and Pseudomonas spp. were encountered in all samples. E. coli, Vibrio spp. and Salmonella spp. were also found in a majority of the samples. Besides, all the isolates were found to be multidrug-resistant. The isolates showed almost 100% resistance against Cefuroxime and Cefixime. A higher proportion of resistance was also reported against Vancomycin and Azithromycin. Whereas, Gentamycin, Ceftriaxone, Colistin and Levofloxacin sensitivity were found in all the isolates. Such chicken-based frozen snack items contaminated with multi-drug resistant microorganisms could be potential vehicles for transmitting food-borne diseases.

scholarly journals Isolation of Multi-Drug Resistant Potential Pathogenic Bacteria From Blow Fly Collected From Different Areas of Dhaka City

2021 ◽  
Vol 49 (2) ◽  
pp. 205-214
Author(s):  
Tangin Akter ◽  
Samira Jahan ◽  
Sangita Ahmed ◽  
Shanjida Sultana ◽  
Shefali Begum
Keyword(s):  
Pathogenic Bacteria ◽  
Dhaka City ◽  
Blow Fly ◽  
Salmonella Spp ◽  
Blow Flies ◽  
Fish Market ◽  
Shigella Spp ◽  
Bacillus Spp ◽  
Staphylococcus Spp ◽  
Klebsiella Spp

The research work was conducted for isolation and identification of potential pathogenic bacteria from blow fly (Calliphora spp.) and to investigate their antibiotic susceptibility. For this purpose, 20 blow flies were collected from 4 selected areas (Curzon Hall, Dhaka Medical College Hospital, Fruit Store and Fish Market) of Dhaka city. A total of 40 samples (20 from external surface and 20 from alimentary gut homogenates) were prepared from the blow flies. Among the total 20 flies, 3(15%) were collected from Curzon Hall and 5(12.5%) isolates were obtained from them; 6(30%) flies were collected from DMCH which had 11(27.5%) isolates; 5(25%) flies were collected from fruit stores and 10(25%) isolates were collected from there; 6(30%) flies were collected from fish market and 14(35%) isolates were collected from there. The isolated bacteria were presumptively identified as Escherichia coli, 12 (30%); Salmonella spp., 8 (20%); Shigella spp., 4(10%); Enterobacter spp., 3 (7.5%); Klebsiella spp., 2 (5%); Bacillus spp., 7 (17.5%) and Staphylococcus spp., 4 (10%) in number which were based on morphology as observed under microscope as well as cultural and biochemical properties. All of these isolates were resistant to various antibiotics. Bacterial susceptibility showed that E. coli isolates were highly resistant (66.6%) to Penicillin, Salmonella spp. mostly resistant (62.5%) to Penicillin and Tetracycline. Klebsiella spp. Isolates were 100% resistant to Penicillin and 100% sensitive to Ciprofloxacin, Imipenem and Tetracycline. In case of Shigella spp. and Enterobacter spp., 75% and 100% isolates were resistant to Penicillin respectively. Among the Staphylococcus spp. isolates, 75% were resistant to Ampicillin and Penicillin. In case of Bacillus spp. isolates, 57.2% were resistant to Penicillin and 100% sensitive to Imipenem. Antibiotic resistance of these species affect our human health also. This study demonstrated the potential of blow flies as a vector of various pathogenic microorganisms and a mode of transmission of antibiotic resistance. Bangladesh J. Zool. 49 (2): 205-214, 2021

scholarly journals Microbiological analysis and detection of anti-bacterial activity of Centella asiatica and Aloe vera samples collected from different areas of Dhaka city, Bangladesh

2017 ◽  
Vol 6 (1) ◽  
pp. 39-43 ◽  
Author(s):  
Susmita Bhowmik ◽  
Runa Akter Chowdhury ◽  
Md Aftab Uddin
Keyword(s):  
Antibacterial Activity ◽  
Aloe Vera ◽  
Centella Asiatica ◽  
Bacterial Count ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Salmonella Spp ◽  
Fungal Load ◽  
Bacillus Spp ◽  
Klebsiella Spp

The present study was performed to detect the presence of contaminating microorganisms in two commonly available herbal samples (Centella asiatica and Aloe vera) collected from different areas of Dhaka city, Bangladesh and to assess their antibacterial activity. Out of twenty samples (having ten samples of each categories) studied; the range of total viable bacterial count was approximately 103 to 108 cfu/g. Presence of Staphylococcus aureus was found in all the samples, followed by Klebsiella spp. in 15 samples, Pseudomonas spp. in 14 samples, Bacillus spp. in 12 samples, Escherichia coli in 9 samples and Vibrio spp. in 7 samples. Salmonella spp. was detected in neither of the sample. 17 samples showed a high fungal load up to 107 cfu/g. Antibacterial activity of C. asiatica samples was demonstrated against eight laboratory isolates. Only four C. asiatica samples showed activity against Klebsiella spp. On the contrary, Aloe vera samples (12-14) showed antibacterial activity only against Staphylococcus spp.Stamford Journal of Microbiology, Vol.6(1) 2016: 39-43

Microbiological analysis of popular spreads used in restaurants inside Dhaka city, Bangladesh

2020 ◽  
Vol 10 (1) ◽  
pp. 16-19
Author(s):  
Tasnia Ahmed ◽  
Md Aftab Uddin
Keyword(s):  
Raw Materials ◽  
Peanut Butter ◽  
Food Additives ◽  
Microbiological Analysis ◽  
Dhaka City ◽  
Acceptable Result ◽  
Fast Foods ◽  
Public Consumption ◽  
Viable Bacteria ◽  
Almost All

Spreads are used widely for making the fast foods more amazing and tasty. Varieties and cross combinations of ingredients are used to make many flavours of fast foods. Different restaurants prepare their signature spreads for attracting people but this can cause opposite result if not prepared using high quality raw materials and proper hygienic conditions are not maintained. Current study was conducted on ten different types of spreads (pesto, cilantro, queso, tomato sauce, peanut butter, honey, mustard, cream cheese, chocolate sauce and butter) which are used by the local restaurants in Dhaka city, Bangladesh. Almost all the samples (nine out of ten) harbored total viable bacteria exceeding the standard limit. Four spread samples showed high fungal count (102 cfu/gm). Four samples showed to be positive (˃101/ml recommended acceptable count) for Escherichia coli and Klebsiella spp. which indicates that these spread cannot be recommended for public consumption. Among all the samples examined, only chocolate sauce showed acceptable result without the presence of coliforms, Staphylococcus spp. and Pseudomonas spp. This finding suggests proper maintenance of sanitation in spread preparation and selling area. A proper guidelines and monitoring can help keep up the quality of food additives. Stamford Journal of Microbiology, Vol.10 (1) 2020: 16-19

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