scholarly journals The effect of plant hormone indoleacetic acid (IAA) on hematological and biochemical parameters in mice

1970 ◽  
pp. 5-8 ◽  
Author(s):  
MH Morshed ◽  
M Shamm Hossain ◽  
M Anwar Habib ◽  
MM Moinuddin Ahmed ◽  
M Ibrahim ◽  
...  
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
Plant Hormone ◽  
Indoleacetic Acid ◽  
White Blood Cells ◽  
Control Group ◽  
Differential Count ◽  
General Observation ◽  
Hematological And Biochemical Parameters ◽  
Serum Glutamate

The subacute toxicity of plant hormone indoleacetic acid (IAA) was studied on Swiss albino mice. The studies include the gross general observation such as changes in body weight, hematological profiles [total count of red blood cells (RBC) and white blood cells (WBC), differential count of WBC, platelet count, hemoglobin (Hb)%], biochemical parameters of blood [serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), serum alkaline phospatase(SALP), creatinine] and histopathology of the liver, kidney, lung, spleen, heart and brain of both control and experimental day-1 for consecutive 14 days, showed no significant change of hematological and biochemical parameters. No abnormalities were also found in the histopathology of the liver, kidney, lung, spleen, heart and brain in the experimental group of animals following same dose when compared with control group. This preliminary toxicological study suggests that the plant hormone indoleacetic acid (IAA) may be used safely for agricultural purposes and as an external preservative DOI: 10.3329/bjpp.v22i1.3561 Bangladesh J Physiol Pharmacol 2006; 22(1/2) : 5-8  

scholarly journals Could the Vitacel R200 improve the Immune Response and Hemato-Biochemical Parameters of Rainbow Trout (Oncorhynchus mykiss) challenged with Aeromonas hydrophila?

2020 ◽  
Vol 10 (03) ◽  
pp. 332-336
Author(s):  
Ali Kane ◽  
Nabeel Al-Sharafi ◽  
Sabah Hamad
Keyword(s):  
Rainbow Trout ◽  
Oncorhynchus Mykiss ◽  
Aeromonas Hydrophila ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Control Group ◽  
Serum Total Protein ◽  
Rainbow Trout Oncorhynchus Mykiss ◽  
Immune Parameters

The fermentable fiber, Vitacel R200 is industrial compound cellulose exerting many beneficial health effects at the hosts. In this study, rainbow trout (Oncorhynchus mykiss) fingerling (120 ± 4.5 grams) were fed dietary 1% (10 g/kg feed) Vitacel R200 for 7 weeks, then challenged with Aeromonas hydrophila. Two weeks after the challenge, hemato-biochemical and immune parameters in the challenged fish were studied. The lysozyme and complement activity, as well as, white blood cells (WBCs), hemoglobin (Hb%), percentage of neutrophil and eosinophil in the fish fed Vitacel were significantly increased compared with the control group. Furthermore, the serum total protein, albumin, Na+, K+, P, and Ca++ concentrations in the fish fed diet containing 1% Vitacel were significantly increased, while alanine aminotransferase (ALT), alkaline phosphatase (ALP), and aspartate aminotransferase (AST), as well as, cortisol, cholesterol, and glucose concentration were significantly decreased in comparison to the control group. These results concluded that dietary Vitacel could improve the hemato-biochemical and immune parameters, as well as, the stress response in O. mykiss infected with A. hydrophila.

scholarly journals EFFECT OF THE "UNICOCCIDUM" ON HEMATOLOGICAL AND BIOCHEMICAL PARAMETERS OF PIGLETS

2020 ◽  
pp. 496-501
Author(s):  
Cheskidova ◽  
Shelyakin ◽  
Bliznetsova ◽  
Semenov
Keyword(s):  
Therapeutic Dose ◽  
Biochemical Parameters ◽  
Clinical Status ◽  
White Blood Cells ◽  
New Drugs ◽  
Clinical State ◽  
Control Group ◽  
Group I ◽  
Negative Effect ◽  
Hematological And Biochemical Parameters

For the treatment of eimeriosis, drugs that are different in their chemical structure and direction of action are used, which can have a negative effect on productive animals. When conducting preclinical studies of new drugs, we carried out a study of the harmlessness (tolerance) of the drug Unicoccidum in piglets. Healthy of 2 months old animals (n = 18) were selected for this purpose. Piglets of group I – for the control, they didn’t receive the drug. Animals of group II were orally individually administered Unicoccidum at a dose of 2.0 ml/kg (therapeutic dose), and III group – at a dose of 10.0 ml/kg (5-fold therapeutic dose). The toxic effect of the drug was evaluated by the clinical state of the animals, hematological (erythrocytes, hemoglobin, white blood cells, leukogram) and biochemical parameters (total protein, urea, creatinine, AST, ALT, AP, γ-GT, cholesterol, glucose, total calcium, inorganic phosphorus). It was found that the use of Unicoccidum in the studied doses did not have a negative effect on the clinical status of piglets. Single administration of Unicoccidum at doses of 2.0 ml/kg and 10.0 ml/kg did not significantly differ in hematological and biochemical parameters of piglets' blood from animals from the control group, and the fluctuations in the values of these indicators were within the physiological norm for this animal species. This can serve as a proof of its good tolerance and harmlessness.

scholarly journals Analysis of phenolic compounds and immunomodulatory activity of areca nut extract from Aceh, Indonesia, against Staphylococcus aureus infection in Sprague-Dawley rats

2020 ◽  
Vol 13 (1) ◽  
pp. 134-140
Author(s):  
Liza Meutia Sari ◽  
Rachmi Fanani Hakim ◽  
Zaki Mubarak ◽  
Andriyanto Andriyanto
Keyword(s):  
Staphylococcus Aureus ◽  
Blood Cells ◽  
White Blood Cells ◽  
Control Group ◽  
Immunomodulatory Activity ◽  
Areca Nut ◽  
Sprague Dawley ◽  
Phytochemical Content ◽  
Sprague Dawley Rats ◽  
Serum Glutamate

Aim: The aim of the study was to investigate the immunomodulatory activity of areca nut extract. The phytochemical content and phenolic composition of the extract were also determined. Materials and Methods: An extract of areca nut was prepared using 96% ethanol and subsequently screened for phytochemical content using a high-performance liquid chromatography (HPLC) method. The immunomodulatory activity of the extract was tested in 35 Sprague-Dawley rats, divided into four groups: One control group and three experimental groups in which the rats received 500, 1000, or 1500 mg/kg of oral areca nut extract biweekly (BW). The extract was orally administered 14 days before the intraperitoneal challenge with Staphylococcus aureus (1×108 CFU/mL). On the 14th day of the experiment, rats in all the four groups were sacrificed. Measurement of the levels of red blood cells, hematocrit (Hct), hemoglobin (Hb), white blood cells (WBCs), lymphocytes, monocytes, neutrophils, basophils, eosinophil, and macrophages were recorded. The activities of serum glutamate oxalate transaminase, serum glutamate pyruvate transaminase, urea, and creatinine were also determined. Results: Areca nut was found to contain an alkaloid, tannin, and flavonoid compounds. HPLC analysis revealed the presence of catechin as the major compound along with quercetin. Administration of areca nut extract in rats infected with S. aureus produced a significant increase in the concentration of WBC but did not affect Hct, Hb, and other cell types. Among the different doses tested, 1000 mg/kg BW was found to be most effective in cellular immunity models. No harmful effects on the liver and kidney functions were observed. Conclusion: The antioxidant activity of areca nut might be attributed to the presence of catechin and quercetin. Administration of areca nut extract increased the number of WBCs and improved the activity and capacity of macrophages significantly in rats infected with S. aureus.

scholarly journals Comparative Effects of Using New Multi-Strain Synbiotics on Chicken Growth Performance, Hematology, Serum Biochemistry and Immunity

Animals ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1555
Author(s):  
Artur Żbikowski ◽  
Karol Pawłowski ◽  
Katarzyna Śliżewska ◽  
Beata Dolka ◽  
Joanna Nerc ◽  
...  
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Blood Cells ◽  
Biochemical Parameters ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Serum Biochemistry ◽  
Control Group ◽  
Normal Ranges ◽  
Rbc Count

In this study, the influence of new multi-strain synbiotics on chicken growth performance, hematology, serum biochemistry and immunity was explored. Each synbiotic preparation (A, B and C) comprised three, four or five strains of Lactobacillus sp., respectively, as well as S. cerevisiae and inulin. All strains used in the synbiotics originated from wild-type strains from animal farms in Poland. Six groups of chickens, ROSS 308 line, were fed with three different synbiotics at a dose of 0.5 g/1 kg of feed. Body weight, as well as the biochemical and hematological parameters of the animals in each study group, were determined on the 7th, 14th, 28th and 42nd day of life. Body weight on day 42 differed between groups and was the highest in control group. This group also had the highestfeed conversion ratio (FCR) value. All measured biochemical parameters were in the normal ranges for poultry; however, we observed a lower alkaline phosphatase (AP) concentration on day 7 in the groups fed with synbiotics, which correlated with a lower level of triglycerides in those groups. The aspartate transaminase (AST) concentration was significantly lower in all groups on day 42 in comparison with the control. On day 7, the control group showed the highest concentration of Ca, K and P. Other parameters did not differ significantly throughout the experiment. All groups showed a similar tendency of increase in the red blood cells (RBC) count according to the age of the birds. Every white blood cells (WBC) population showed differences in the proportions between T and B lymphocytes. The T cell and monocyte counts increased until day 28 in all groups. The results showed that our newly developed synbiotic formulas do not have any unfavorable influence on chicken health and may modulate immune response and biochemical parameters. However, this hypothesis needs to be evaluated in future experiments.

scholarly journals Changes in goslings’ blood pattern under the influence of different doses additives of selenium in forages

2020 ◽  
Vol 22 (92) ◽  
pp. 50-55
Author(s):  
S. V. Sobolieva ◽  
B. V. Gutyj ◽  
O. I. Sobolev
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Immune Defense ◽  
The Body ◽  
Practical Significance ◽  
Poultry Feed ◽  
Control Group ◽  
Wide Range ◽  
Different Doses

According to many scientists, the list of trace elements that are currently used in compound feeds for various types of poultry is clearly insufficient. In recent years, applied research has been conducted to determine the physiological needs of poultry for certain mineral elements that perform important biochemical functions in the body. This also applies to such biotic ultramicroelement as selenium. Scientific research of domestic and foreign scientists in the field of physiology, biochemistry, medicine and veterinary medicine has proved that selenium is a trace element with a fairly wide range of physiological and biochemical effects. When developing and theoretically justifying optimal norms for introducing selenium into poultry feed, it is necessary to evaluate not only its productive qualities, but also the blood picture. The question of the influence of selenium on changes in blood parameters in the poultry body is a great theoretical and practical significance because it allows us to expand our knowledge of its biological role and explain the data obtained in experiments. In scientific and economic experience has been studied the influence of additives of different doses of selenium in compound feed on the morphological and biochemical parameters of the goslings' blood raised for meat. During the experiment, feeding of goslings of all groups were carried out with dry feed mixes in accordance with existing norms. The goslings of the first control group did not receive selenium supplementation. The poultry of the second experimental group were additionally fed selenium at the rate of 0.2 mg/kg, the third of 0.3 and the fourth of 0.4 mg/kg. It is established that the additive in the feed of different doses of selenium stimulate hemocytopoiesis are added to different compound feeds, this is achieved by a homogeneous tendency per day, within physiological values in the goslings' peripheral blood number of red blood cells (by 1.7–3.9 %), white blood cells (by 2.1–3.2 %) and hemoglobin (by 2.4–8.6 %). Immune defense mechanisms are activated, which is manifested in an increase in the level of total protein (by 1.4–3.8 %) and the concentration of immunoglobulins in the blood serum (by 3.2–9.7 %). It is found changes in the content of total glutathione and its reduced form in the blood indicate a positive effect of selenium on the non-enzymatic element of the antioxidant defense system of the poultry's body. The addition of selenium to compound feed at a dose of 0.3 mg/kg had a significant effect on the morphological and biochemical parameters of the goslings' blood raised for meat.

scholarly journals The effect of twinship on selected hematological and biochemical parameters in late pregnant ewes

2021 ◽  
Vol 77 (10) ◽  
pp. 6578-2021
Author(s):  
Yenilmez K. ◽  
Arslan S. ◽  
Kiliç S. ◽  
Atalay H.
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
Ultrasound Examination ◽  
Hematological Parameters ◽  
White Blood Cells ◽  
Negative Energy ◽  
High Positive Correlation ◽  
Blood Samples ◽  
Positive Correlation ◽  
Hematological And Biochemical Parameters

This study aimed to determine the effect of twinship on the hematological and biochemical parameters in ewes of the Kıvırcık breed commonly raised in the Thrace region of Turkey. The study material consisted of 30 Kıvırcık ewes at days 130-150 of gestation, aged 3-5 years, weighing 55.61 ± 0.76 kg. After ultrasound examination, the ewes included in the study were divided into a single-pregnant group (n = 15) and a twinpregnant group (n = 15), and their blood samples were taken. The levels of red blood cells (RBC), hematocrit (HCT), hemoglobin (HGB) white blood cells (WBC), lymphocytes (LYM), monocytes (MON), non-esterified fatty acid (NEFA), β-hydroxybutyrate (BHBA), glucose, cholesterol, total protein, aspartate aminotransferase (AST), alanine aminotransferase (ALT) were determined in their blood samples. It was found that, although twinship did not affect hemogram values in ewes (p > 0.05), the biochemical parameters, BHBA, ALT, and AST values, were significantly affected by twinship (p <0.05). A high positive correlation was found between BHBA and NEFA. Thus it was concluded that twin pregnancy did not cause any significant changes in hematological parameters in Kıvırcık ewes. However, the significantly higher BHBA in twin-pregnant ewes and the high positive correlation between BHBA and NEFA indicate that the negative energy balance started to affect these animals, and a carbohydrate deficiency emerged. Therefore, twin-pregnant ewes should be monitored closely to reduce the risk of ketosis.

Influence of increased doses of fenbendazole supramolecular complex on sheep’s organism

2018 ◽  
Vol 12 (2) ◽  
pp. 46-52 ◽  
Author(s):  
A.I. Varlamova
Keyword(s):  
Blood Cell ◽  
Cell Count ◽  
Biochemical Parameters ◽  
Negative Influence ◽  
Supramolecular Complex ◽  
Control Group ◽  
Blood Cell Count ◽  
Russian Scientific ◽  
Russian Scientific Research Institute ◽  
Hematological And Biochemical Parameters

The purpose of the research: study of the influence of increased doses of fenbendazole supramolecular complex (FSMC) on sheep’s organism. Materials and methods. The experiment was carried out at the Podolsk Department of All-Russian Scientific Research Institute of Fundamental and Applied Parasitology of Animals and Plants named after K. I. Skryabin on 20 manorial invasion-free sheep aged 2-3 years old. Animals were divided according to the principle of analogues into 4 groups, 5 heads in each group. Animals of the 1, 2 and 3 group were orally administered with FSMC given as a single dose of 2, 6, 10 mg/kg, respectively, according to the active substance, i.e in therapeutic and in a dose increased by 3 and 5 times. Sheep of the fourth group didn’t receive the drug and they were as control. Study of clinical, hematological and biochemical parameters of animals from all groups was conducted 1 day before and in 1, 3, 5 days after administration of the drug by means of standard methods. Results and discussion. FSMC in therapeutic dose as well as in a dose increased by 3 and 5 times doesn’t have negative influence on clinical, hematological and biochemical parameters of the sheep. State of the sheep, which received the drug in doses of 20, 60, 100 mg/kg, was within the physiologically normal state and didn’t differ from the state before administration of the drug and from the animals of the control group. Drug security index exceeds 5. Red blood cell count, white blood cell count, hemoglobin count, leukogram parameters as well as biochemical parameters of blood: activity of alkaline phosphatase and amylase, bilirubin, creatinine, urea and glucose counts were within normal limits and didn’t differ from the parameters of the control animals.

scholarly journals Influence of Pesticide on Biochemical and Hematological Parameters in Beninese Vegetable Farmers

2018 ◽  
Vol 9 (1) ◽  
pp. 65
Author(s):  
Lucie AYI-FANOU
Keyword(s):  
Blood Cell ◽  
Biochemical Parameters ◽  
Mean Corpuscular Volume ◽  
Hematological Parameters ◽  
Control Group ◽  
Study Group ◽  
Blood Samples ◽  
Vegetable Farmers ◽  
Insignificant Difference ◽  
Hematological And Biochemical Parameters

In several countries, a lot of researches have shown the toxicity of pesticides on farmers. Meanwhile, few of them have dealt with vegetable farmers in Benin. Therefore, this study has been carried out to assess the effects of pesticides on vegetable farmers’ health. This study is conducted on 30 vegetable farmers who used pesticides and 20 subjects as control group. Pesticides used by vegetable farmers and their risky behavior were investigated. Their blood samples were collected and some tests were performed for hematological and biochemical parameters. For the statistical analysis of the results, Student’s test was used. Our investigations revealed that vegetable farmers of Benin were exposed to different active ingredients of pesticides such as (Abamectin, Acetamiprid, Chlorpyriphos ethyl, Cyfluthrin, Cypermethrin, Emamectin benzoat, Flubendiamid, Lambdacyhalothrin, Mancozeb, Profenofos, Spinosad and Spirotétramate). The hematological parameters showed significant decrease in White blood cell (WBC), Red blood cell (RBC), Hemoglobin (HGB), Hematocrit (HTC), Mean Corpuscular Volume (MCV), Platelet (PLT) count among study group. The enzymatic activities of Aspartate Aminotransferase (AST), Alanine Aminotransferase (ALT) have significantly raised between vegetable farmers and the control group, while the serum concentration in urea and creatinine showed insignificant difference in the study group. These results suggest that pesticides have adverse effects on vegetable farmers of South of Benin.

scholarly journals Some Immunological Parameters Paraw in Women with vaginal candidiasis

2007 ◽  
Vol 4 (2) ◽  
pp. 191-194
Author(s):  
Baghdad Science Journal
Keyword(s):  
T Lymphocytes ◽  
Immune Responses ◽  
Blood Cells ◽  
Vaginal Candidiasis ◽  
Control Group ◽  
Differential Count ◽  
Immunological Parameters ◽  
Phagocytosis Index

This study was conducted to know some Immune responses in women with vaginal candidias is by Candidia species. Hemoglubin (Hb) was decreased significantly in patient women comparing with control group. The differential count of Blood cells revealed that neutrophils and monocytcs were increased significantly in patient women comparing with control group. The percentage of the Phagocytosis index was decreased significantly in patient women (66.45 + 15.05) comparing with control group (73.72 + 3.77) and the T- lymphocytes were decreased also in patient women (57.75 + 18.787) comparing with control group (74.25 + 7.759).

scholarly journals Haematological and Serum Biochemical Profile of Growing Yankasa Ram Lambs Fed Diets Containing Graded Levels of Sesame Residue

2020 ◽  
Vol 2 (5) ◽  
Author(s):  
Y. Garba ◽  
E. A. Adeola
Keyword(s):  
Blood Cells ◽  
Biochemical Parameters ◽  
White Blood Cells ◽  
Haemoglobin Concentration ◽  
Serum Biochemical Parameters ◽  
Corpuscular Haemoglobin ◽  
Randomized Design ◽  
Serum Biochemical ◽  
Mean Corpuscular Haemoglobin ◽  
Ram Lambs

An experiment was carried out to test the effect of Sesame residue (SR) inclusion in the diets of growing Yankasa ram lambs on haematological and serum biochemical parameters. Twelve (12) Yankasa ram lambs with initial body weight of 20.08±3.08kg were allotted to three dietary treatments containing 0, 20 and 30% SR (A, B and C). A completely randomized design was used for the study which lasted twelve (12) weeks. The haemetology results revealed that the white blood cells (12.85-16.85 109/L), red blood cells (1.49-2.04 1012/L), haemoglobin, mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH) (39.85-48.00 pg), mean corpuscular volume (MCV) and lymphocytes differed significantly (P<0.05) among the treatments. Lambs fed 30% SR had the highest MCV, MCH, MCHC and lymphocyte values. Serum biochemical analysis results revealed that lambs fed 20% and 30% SR had higher alkaline phosphatase, total protein and globulin values than lambs fed 0%SR. However, SR inclusion in the diets did not affect calcium and phosphorus levels of the lambs. In conclusion, including sesame residue at 30% in the diets of growing Yankasa ram lambs had no observable adverse effect on haematological and serum biochemical parameters.

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