scholarly journals Environment related variations in the semen characteristics of bulls used for Artificial Insemination (AI) programme in Bangladesh

1970 ◽  
Vol 26 ◽  
pp. 81-88 ◽  
Author(s):  
Md Jalal Uddin Sarder
Keyword(s):  
Relative Humidity ◽  
Ambient Temperature ◽  
Artificial Insemination ◽  
Sperm Concentration ◽  
Dairy Farm ◽  
Sperm Cells ◽  
Motile Sperm ◽  
Semen Characteristics ◽  
Mass Activity ◽  
Motility Of Sperm

The present study assessed the semen characteristics of bulls used for Artificial Insemination (AI) programme in Bangladesh with regard to season, ambient temperature and relative humidity. A total of 3720 semen samples from 71 bulls were collected from Central Cattle Breeding Station and Dairy Farm (CCBSDF), Savar, Dhaka, Rajshahi Dairy and Cattle Improvement Farm (RDCIF), Rajabarihat, Rajshahi and District Artificial Insemination Centre (DAIC), Rajshahi during 1995 - 2002. Semen was collected by artificial vaginal method once a week. Different seasons viz. spring, summer, autumn and winter had significant effect (P<0.05) on colour, density, mass activity, sperm concentration, sperm motility, total sperm cells/ejaculate, total number of motile sperm cells/ejaculate, number of semen doses per collection and postfreezing motility of sperm. The maximum values of these semen characteristics were found in summer. Ambient temperature groups of <21°C, 21-25°C, 25- 29°C and >29°C had significant (P<0.05) effect on all the semen characteristics except for volume of semen. The highest volume of semen was found at >29°C and the lowest at 25-29°C. The total number of sperm cells/ejaculate, total number of motile sperm cells/ejaculate and number of semen doses per collection were found in >29 °C temperature groups. Relative humidity groups of <65%, 65-75%, 75-85% and >85% had significant effect on all the semen characteristics excluding for volume of semen and number of semen doses per collection. The largest volume of semen, colour, mass activity, total sperm cells/ejaculate, total motile sperm cells/ejaculate and number of semen doses/collection were found in >85% relative humidity and the smallest in 65%-75% relative humidity. Finally, this study recommended that, summer season, ambient temperature groups of >29°C and Relative humidity groups of >85% are suitable for excellent semen characteristics. Key words: AI bulls; semen characteristics; season; temperature; relative humidity Univ. j. zool. Rajshahi Univ. Vol. 26, 2007. pp. 81-88

scholarly journals Effects of Climatic Conditions on Bovine Semen Characteristics

2017 ◽  
Vol 65 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Zuzana Biniová ◽  
Jaromír Ducháček ◽  
Luděk Stádník ◽  
Martina Doležalová ◽  
Radim Hájek
Keyword(s):  
Relative Humidity ◽  
Ambient Temperature ◽  
Artificial Insemination ◽  
Sperm Concentration ◽  
Climatic Conditions ◽  
Bovine Semen ◽  
Required Quality ◽  
Daily Relative Humidity ◽  
Artificial Vagina ◽  
Qualitative And Quantitative Characteristics

The present study assessed the effects of climatic conditions and year‑period (month) on both qualitative and quantitative characteristics of bovine semen in Czech Republic. The semen was collected twice a week using artificial vagina at artificial insemination center Hradištko in period 2011 – 2013. The effects of collection month, average daily ambient temperature and average daily relative humidity on volume, sperm concentration and progressive motility above head of collected semen and also on number of produced artificial insemination straws of required quality were analyzed using statistical analysis (procedures: UNIVARIATE, STEPWISE, GLM) in software SAS 9.3 (SAS/STAT® 9.3, 2011). Collection month, average daily ambient temperature, average daily relative humidity, and breed had a significant effect (P < 0.05) on all evaluated characteristics. The maximum values of motility and sperm concentration were found in March and in April. The highest volume of collected semen was found in July and in September and the lowest in November, when also the lowest number of straws was produced. Motility and sperm concentration were significantly influenced by average daily ambient temperature and average daily relative humidity, where the maximum values were recorded in average daily ambient temperature over 14.85 °C and average daily relative humidity over 72.05 %. The results are important to the management of artificial insemination center responsible for planning of semen collection, processing and artificial insemination straws production.

scholarly journals The effect of freezing on cryosurvival of yak sperm

2018 ◽  
Vol 15 (2) ◽  
pp. 215-218
Author(s):  
S Deori
Keyword(s):  
Liquid Nitrogen ◽  
Sperm Motility ◽  
Artificial Insemination ◽  
Sperm Concentration ◽  
Egg Yolk ◽  
Mean Values ◽  
Frozen Semen ◽  
Sperm Abnormality ◽  
Semen Characteristics ◽  
Live Sperm

A study was carried out to study the effect of freezing on cryosurvival of yak semen. Artificial insemination in yak is still in infancy. Semen cryopreservation and use of artificial insemination can be applied in yak husbandry for conservation and rapid multiplication of superior germplasm. Semen was collected from four adult yak bulls using artificial vagina method managed under uniform conditions. A total of 40 ejaculates comprising of 10 ejaculates each bull were collected following twice a week schedule and evaluated for fresh semen characteristics. The fresh yak semen characteristics viz. ejaculate volume (ml), mass activity (0-4), initial sperm motility (%), sperm concentration (x 106/ml), live sperm (%), sperm abnormality (%) and intact acrosome (%) were 3.10 ± 0.18, 3.53 ± 0.96, 83.89 ± 2.87, 1180.22 ± 42.32, 77.63 ± 4.23, 8.45 ± 3.33 and 93.61 ± 3.78 respectively. The ejaculates were diluted (1:10) with Tris extender consisting of 6.4 ml glycerol and 20 ml of fresh egg yolk. Straws were equilibrated at 5°C for 4 hours followed by exposure to liquid nitrogen vapour for 10 minutes and finally transferred to liquid nitrogen container for storage. The cryosurvival rate was studied after 7 days of storage in liquid nitrogen. The frozen semen was thawed in warm water (37°C) for 30 seconds for evaluation. Mean values of postthaw sperm motility (%), live sperm (%) and intact acrosome (%) in yaks were 55.67 ± 4.67, 65.62 ± 3.23 and 89.26 ± 3.67 respectively. In conclusion, yak semen has a better cryosurvival while freezing in tris extender with 6.4 per cent glycerol and 20 per cent egg yolk following an equilibration period of 4h.SAARC J. Agri., 15(2): 215-218 (2017)

Semen characteristics of Awassi rams

1969 ◽  
Vol 73 (2) ◽  
pp. 311-314 ◽  
Author(s):  
K. H. Juma ◽  
F. Dessouky
Keyword(s):  
Methylene Blue ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Sperm Number ◽  
Sperm Production ◽  
College Of Agriculture ◽  
Sperm Abnormality ◽  
Methylene Blue Reduction ◽  
Semen Characteristics ◽  
Mass Activity

SUMMARYOn the College of Agriculture farm in Abu-Ghraib 147 ejaculates were collected from three docked and four normal Awassi rams born during November 1962. The work covered a period from 1 April 1967 to 31 March 1968.Nine semen characters, namely, volume, mass activity, individual motility, sperm concentration, sperm number, pH, methylene blue reduction time and the percentages of abnormal and dead sperms, were studied. The effect of season and docking on these characters was investigated.Seasonal variation was observed in all traits studied. On the whole, semen quality was poorest during winter and best during summer. The effect of docking was more pronounced on sperm concentration, sperm number and percentage of abnormal sperms. Docking appeared to increase sperm production and reduce sperm abnormality.

scholarly journals Influence of microclimatic conditions on the daily production of dairy cows

2013 ◽  
Vol 29 (1) ◽  
pp. 45-51 ◽  
Author(s):  
D. Kucevic ◽  
M. Plavsic ◽  
S. Trivunovic ◽  
M. Radinovic ◽  
V. Bogdanovic
Keyword(s):  
Relative Humidity ◽  
Ambient Temperature ◽  
Dairy Cows ◽  
Milk Yield ◽  
Dairy Farm ◽  
Comfort Level ◽  
Daily Production ◽  
Microclimatic Conditions ◽  
Daily Milk Yield ◽  
Daily Milk

The aim of this paper was to determine the microclimatic conditions (ambient temperature and relative humidity) in dairy farm, as well as to evaluate the effect and significance of temperature - humidity index (THI) values on the daily milk yield. The observation of microclimatic parameters was conducted in the period from 9.03.2012 to 6.05.2012. The study included 136 Holstein Friesian cows. The estimation of the effect of THI on daily production of dairy cows was defined by applying fixed-effect statistical model. Average ambient temperature during measuring months amounted to 15.6?C (ranging from 7.2? C to 24.6?C), while the average value of relative humidity was 56.33% (ranging from 40.30% to 81.80%). During the study, the mean value of THI was 58.93 (ranging from 47.08 to 70.13) and didn?t exceed the critical comfort level of 72. All tested fixed-factors were statistically affected the daily milk yield (p <0.01). For each unit of increase in the value of the THI, the amount of milk decreased by 0.05344 kg. This confirmed the importance of regular recording of THI values and microclimatic conditions as a unique indicator of thermal stress in dairy farm.

scholarly journals Individual Variation in Semen Characteristics of Murrah Buffalo Bulls

2020 ◽  
Vol 9 (3S) ◽  
pp. 58-60
Keyword(s):  
Sperm Motility ◽  
Individual Variation ◽  
Artificial Insemination ◽  
Recovery Rate ◽  
Sperm Concentration ◽  
Murrah Buffalo ◽  
Frozen Semen ◽  
Semen Characteristics ◽  
The Individual ◽  
Fresh Semen

This study evaluated the variation in semen characteristics between two individual Murrah buffalo bulls. Two hundred and ninety semen samples were involved in this study. The results showed that most of the semen had a milky white color with moderate consistency. The Bull B1 tended to have higher proportion of creamy color and moderate consistency than the Bull B2. The ejaculatory volume is varied (P<0.01) between Bull B1 and Bull B2. However, semen pH and sperm concentration of Bull B1 and B2 were equal (P>0.05). The individual bull had a highly significant effect (P<0.01) on sperm motility of fresh semen. The individual variation (P<0.05) also found on sperm motility of before freezing. Whereas, sperm motility of post-thawing semen and recovery rate value were not significantly influenced (P>0.05). Moreover, it was found that the frozen semen production differed (P<0.01) between two individual bulls, with bull B1 had higher production than the bull B2. This study clearly demonstrates that the frozen semen production of Murrah buffalo is highly dependent on the individual bull. However, the variation in frozen semen production between two bulls studied here is still qualified to be used for artificial insemination.

PSIII-1 Influence of prenatal or postnatal exposure to gossypol from cottonseed meal on semen quality in commercial boars: a preliminary study on feral hog control

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 284-285
Author(s):  
Randi F Benefield ◽  
Richard A Mudarra ◽  
Tsung-Cheng Cheng Tsai ◽  
Christopher R Hansen ◽  
Charles V Maxwell ◽  
...  
Keyword(s):  
Cottonseed Meal ◽  
Semen Quality ◽  
Sperm Concentration ◽  
Sperm Cells ◽  
Motile Sperm ◽  
Postnatal Exposure ◽  
Treatment Groups ◽  
Progressive Motility ◽  
Feral Hog ◽  
Chi Square Analysis

Abstract The objective was to examine the influence of prenatal (Experiment 1) or postnatal (Experiment 2) exposure to gossypol from cottonseed meal (CSM) on semen quality in commercial boars. In Experiment 1, pregnant sows (n = 5) were fed a diet containing 0% (n = 1), 0.04% (n = 2), or 0.08% (n = 2) gossypol between d 56 and 86 of gestation. Boars (n = 11) born to sows in each treatment group (0% gossypol n = 3; 0.04% gossypol n = 4; 0.08% gossypol n = 4) were fed a common diet without CSM, and semen was collected at 269±2 d of age using a live sow in estrus. In Experiment 2, boars (n = 21) were fed a diet containing 0%, 0.02%, or 0.04% gossypol between 63±1 and 105±1 d of age (Initial BW: 19.85±0.43 kg). After the treatment period, boars were fed a common diet without CSM, and semen was collected at 238±7 d of age using a breeding dummy. Sperm cell concentration, percentage of motile sperm cells, and percentage of progressively motile sperm cells were analyzed using the MIXED procedure of SAS with treatment as a fixed effect in Experiment 1 and 2 and dam as a random effect in Experiment 1. In Experiment 2, semen was not successfully collected from every boar; therefore, chi-square analysis was used to assess semen collection status between treatment groups using the FREQ procedure of SAS. In Experiment 1, there was no difference in sperm concentration (P = 0.45), percent motility (P = 0.71), or percent progressive motility (P = 0.27) between treatment groups. In Experiment 2, there was no difference in sperm concentration (P = 0.72), percent motility (P = 0.17), or percent progressive motility (P = 0.87) between treatment groups. No difference was observed in boar collection status between treatment groups (P = 0.77). In conclusion, prenatal or postnatal exposure to gossypol from CSM did not influence semen quality in commercial boars.

scholarly journals Effects of the carbonated drink as an extender on semen characteristics, fertility and hatchability in Nigerian indigenous chicken

2021 ◽  
Vol 66 (2) ◽  
pp. 155-163
Author(s):  
Adeyina Oluwatoba ◽  
Akanbi Samuel ◽  
Okukpe Mathias ◽  
Alli Ibidapo
Keyword(s):  
Egg Yolk ◽  
Test Tube ◽  
Average Weight ◽  
Sperm Cells ◽  
Indigenous Chicken ◽  
Semen Characteristics ◽  
Semen Preservation ◽  
Motility Of Sperm ◽  
And Control ◽  
Carbonated Drink

Semen extenders are liquid diluents that buffer sperm cells and preserve their fertilizing potentials. The commercial carbonated drink (CD) as an extender was evaluated on semen characteristics, fertility and hatchability in Yoruba ecotype chickens (YECs). The fructose of the CD was 1.52?0.05 mg/ml. Under the conditions of 370Celsius, 5% and 10% of CD were added to the egg yolk citrate solution to make 100%. Semen was obtained from ten matured Yoruba ecotype chicken cocks with an average weight of 1.8?0.2 kg. The semen was pooled in a test tube and added to the extenders for preservation at 0, 30 and 60 minutes, respectively, in a factorial design layout. Percentage motility of sperm cells was significantly (p<0.05) higher in 5% CD inclusion compared with 10% CD inclusion and control. Motility decreased with an increase in preservation time across the treatments. The percentage of dead sperm cells decreased (p<0.05) in 5% CD inclusion when compared with 10% CD inclusion and control. The sluggish sperm percentage increased significantly (p<0.05) with semen preservation time. Fertility and hatchability of eggs were significantly (p<0.05) higher in 5% CD inclusion. It was concluded that carbonated drinks at 5% inclusion in an extender could preserve cock sperm cells for 60 minutes with improved fertility and hatchability of eggs.

scholarly journals Assessment of Semen Characteristics Among Three Phenotypes of Chicken Raised in Akko, Gombe State of Nigeria

2021 ◽  
Vol 12 (1) ◽  
pp. 40-48
Author(s):  
Tijjani Haruna Usman ◽  
Saleh Mohammed Sir ◽  
Sadiq Haladu ◽  
Asmad Kari
Keyword(s):  
Artificial Insemination ◽  
Sperm Concentration ◽  
Progressive Motility ◽  
Sperm Abnormalities ◽  
Normal Sperm ◽  
Poultry Breeding ◽  
Semen Volume ◽  
Semen Characteristics ◽  
Abdominal Massage

In poultry breeding program the success of artificial insemination (AI) is highly influenced by  the quality of semen. The aim of the present research was to evaluate the variations in semen characteristics of three phenotypes of chicken of the same specie raised in Akko, Nigeria. A total of 9 cocks from threedifferent phenotypes; Red feathered (n = 3), White feathered (n = 3) and Black feathered (n =3). The semen was evaluated for macroscopic (i.e. semen volume and colour) and microscopic (i.e. sperm concentration, motility and morphology) criteria after being collected by abdominal massage method. There were phenotypes variation (P > 0.05)effects on semen motility, number of live/dead sperm and sperm abnormalities. No differences(P>0.05) were observed on volume, colour, concentration, mass motility and pH of semen. The observed sperm progressive motility was ranged from 90.5 ±1.21% to 95.09±0.82%. The White feathered strain had the highest sperm progressive motility (P<0.05)and highest value for live and normal sperm (93.5±0.63% and 87.90±0.25%), while the black feathered had the least and (92.4±0.73% and 85.5±0.50%) respectively. The Red feathered strain had the highest value for both dead and sperm head defect (9.2±0.33%and 7.0±0.52%). Whilst, the Black feathered strain scored the highest percentage of both sperm tail and neck defects with values ranged from 11.4±0.43%to 14.2±1.08% and 19.4±0.53% to 23.6±0.69%, respectively. This study suggests that there are large variations present in semen characteristics of different phenotypes of cocks; White feathered strain is likely have better semen characteristics compared to Red and Black feathered strains. Therefore, White feathered strain can potentially be used in artificial insemination (AI) for chicken production and improvement.

scholarly journals The impact of adding different levels of egg yolk on the motility and morphology pre and post thaw cryopreservation of goat semen

2019 ◽  
Vol 12 (1) ◽  
pp. 107-115
Author(s):  
Nawfal Mutlak
Keyword(s):  
Liquid Nitrogen ◽  
Breeding Season ◽  
Sperm Motility ◽  
Egg Yolk ◽  
Sperm Cells ◽  
Mass Activity ◽  
Semen Extender ◽  
Motility Of Sperm ◽  
The Impact ◽  
Different Levels

The aim of this study was to examine the effect of different concentrations of egg yolk EY (0%, 10%, and 20%) in the semen extender during the cryopreservation process of goat semen out of the breeding season. A total of 12 ejaculates were collected from six Anglo Nubain dairy bucks as two ejaculates for each buck aged between (1-5) years over a two week period by using Electro-ejaculation (EEJ) during the non-breeding season. Post collection, the semen samples were evaluated for motility and mass activity. Subsequently, the semen samples were initially diluted in Tris solution (without Egg yolk or Glycerol) in order to preserve the motility of sperm cells. The semen samples from each buck were evaluated for pre-freezing motility and morphology then divided into three sub-samples and diluted in Tris extender with T1 (control) 0% EY, T2 10% EY, and T3 20% EY. The semen samples were frozen in liquid nitrogen (-196 C). After thawing, the semen samples were evaluated for sperm motility and morphology. The morphology of sperm did not differ among treatments nor between pre-freezing and post-thawing evaluations. However, the motility of semen diluted with 10% EY was (P<0.05) numerically but not statistically higher than semen diluted with 0% and 20% EY. According to the obtained results of this study, it is recommended that a 10% EY level or less be included in the Tris extender during cryopreservation of goat semen for superior motility and morphology results.

scholarly journals Effect of Selected Factors on Qualitative and Quantitative Semen Parameters of Czech Fleckvieh Bulls

2016 ◽  
Vol 64 (5) ◽  
pp. 1635-1643 ◽  
Author(s):  
Michaela Paldusová ◽  
Tomáš Kopec ◽  
Radek Filipčík ◽  
Martin Hošek ◽  
Ladislav Máchal
Keyword(s):  
Genetic Correlation ◽  
Artificial Insemination ◽  
Sperm Count ◽  
Sperm Concentration ◽  
Semen Parameters ◽  
Reproductive Parameters ◽  
Genetic Characteristics ◽  
Qualitative And Quantitative ◽  
Significant Difference ◽  
Semen Characteristics

The aim of the study was to estimate genetic characteristics for important qualitative and quantitative parameters of Czech Fleckvieh bulls’ ejaculate and to define factors which influence these parameters. a total number of 2,929 entries about samples from 163 bulls of a selected artificial insemination centre were used for calculation. the analysed ejaculate characteristics were: volume of ejaculate, sperm concentration, total sperm count, native sperm activity and activity after thawing. In all evaluated semen characteristics, the age of individual, coefficient of inbreeding, interval between colection and year of colection were statistically significant (P < 0.05). In the case of season of colection, statistically significance (P < 0.01) was proved in all monitored semen parameters, except concetration of spermatozoa where statistically significant difference was not found (P > 0.05). Estimated coefficients of heritability correspond to the values standardly specified for reproductive parameters (h2= 0.003 – 0.14), except for the volume of ejaculate (h2= 0.31). the highest value of genetic correlation was recorded between the volume of ejaculate and the total sperm count (rG= 0.9), on the contrary, the lowest value was between the volume of ejaculate and the sperm concentration (rG= 0.32).

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