scholarly journals Experimental study of amniotic membrane or topical propolis on induced cutaneous wound healing in Wistar rats

2021 ◽  
Vol 15 (3) ◽  
pp. 253-260
Author(s):  
Rafaela Eduarda dos Reis ◽  
Letícia da Silva Rueda ◽  
Bruno Roberto Vidal Tuani ◽  
Cláudia Sampaio Fonseca Repetti ◽  
Camila Dias Porto ◽  
...  

The study of propolis and the amniotic membrane as alternatives to promote the acceleration of healing has grown considerably. These products have shown to be easy to use, have bactericidal or bacteriostatic action and are affordable. The objective of the present study was to evaluate the action of the amniotic membrane of bitches and propolis in the healing of surgical wounds. To evaluate the efficacy of these methods the time for conclusion of the healing process of both groups was compared with a control group. In addition, the inflammatory response of each group was also evaluated to identify which alternative method could induce a better response in the healing process compared to the control group. For this purpose, 27 animals, Rattus norvegicus, variety Wistar, were used. Rats were separated into 3 groups with 9 animals each. Group I represented the 25% alcoholic propolis solution; Group II, Amniotic membrane; Group III was Control group. Wounds were assessed macroscopically for the lesion area, and microscopically for the histological quality of each wound layer and the intensity of inflammation and collagen deposition. After the macroscopic analysis, it was observed that there were no statistical differences among the 3 groups (p> 0.05) relevant to accelerate the healing process in terms of presence of inflammatory cells, fibroblasts, collagen, neovascularization, granulation tissue and re-epithelialization.

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Jéssica Borghesi ◽  
Sara Caceres ◽  
Lara Carolina Mario ◽  
Angela Alonso-Diez ◽  
Ana Carolina Silveira Rabelo ◽  
...  

Abstract Background Tumours in mammary glands represent the most common neoplasia in bitches, as in humans. This high incidence results in part from the stimulation of sex hormones on these glands. Among mammary tumours, inflammatory carcinoma is the most aggressive, presenting a poor prognosis to surgical treatment and chemotherapy. One of the most widely used chemotherapy drugs for breast cancer treatment is doxorubicin (DOXO). Alternative therapies have been introduced in order to assist in these treatments; studies on treatments using stem cells have emerged, since they have anti-inflammatory and immunomodulatory properties. The aim of this study was to evaluate the effects of DOXO and canine amniotic membrane stem cells (AMCs) on the triple-negative canine inflammatory mammary carcinoma cell line IPC-366. Methods Four experimental groups were analysed: a control group without treatment; Group I with DOXO, Group II with AMC and Group III with an association of DOXO and AMCs. We performed the MTT assay with DOXO in order to select the best concentration for the experiments. The growth curve was performed with all groups (I-III) in order to verify the potential of treatments to reduce the growth of IPC-366. For the cell cycle, all groups (I-III) were tested using propidium iodide. While in the flow cytometry, antibodies to progesterone receptor (PR), estrogen receptor (ER), PCNA, VEGF, IL-10 and TGF-β1 were used. For steroidogenic pathway hormones, an ELISA assay was performed. Results The results showed that cells treated with 10 µg/mL DOXO showed a 71.64% reduction in cellular growth after 72 h of treatment. Reductions in the expression of VEGF and PCNA-3 were observed by flow cytometry in all treatments when compared to the control. The intracellular levels of ERs were also significantly increased in Group III (4.67% vs. 27.1%). Regarding to the levels of steroid hormones, significant increases in the levels of estradiol (E2) and estrone sulphate (S04E1) were observed in Groups I and III. On the other hand, Group II did not show differences in steroid hormone levels in relation to the control. We conclude that the association of DOXO with AMCs (Group III) promoted a reduction in cell growth and in the expression of proteins related to proliferation and angiogenesis in IPC-366 triple-negative cells. Conclusions This treatment promoted ER positive expression, suggesting that the accumulated oestrogen conducted these cells to a synergistic state, rendering these tumour cells responsive to ERs and susceptible to new hormonal cancer therapies.


Author(s):  
Фаргат Вагапов ◽  
Fargat Vagapov ◽  
Наталья Гизатова ◽  
Natal'y Gizatova

The purpose of research is increase of beef productivity and beef quality of Simmental calves at introduc-tion in a diet of feeding different doses of the drug Vetosporin suspension. Of the half-yearly animals, 40 males were selected and formed into group IV for the experiment. The differences were only in feeding. The young were fed the studied additive Vetosporin suspension. In this case, the supplement was an addi-tion to the basic diet of animals of the experimental II-IV groups. The volume of the additive added was 0.1; 1.0; and 2 ml per 10 kg of live weight, respectively. Control group I, consumed exclusively a diet that does not contain an additive. Based on the results of the experiment at the age of 18 months. The prevalence of bulls of the experimental live weight over peers was observed, which was 4.4-25.3 kg (0.78-4.67%). The study of the studied indicators after control slaughter in the context of groups showed the superiority of bulls of experimental groups in all the periods studied. It is established that at the age of 15 months. The size of the removable live weight of the youngest of the control group was less by 8.0-19.7 kg (1.75-4.29%) than in the animals of the test groups. In this case, the leading position was occupied by the youngest of group III, the prevalence of which was 0.7-11.7 (0.14 -2.51%). It should be noted that after 3 months a simi-lar picture of the distribution of the studied quantities was observed. As for the output of carcass, we can note the following. Outsider among the animals of the experimental groups was the control group. So the bulls of group I were inferior to those who received the additive by 0.7-1.3%. At 18 months, as well as at 15 months, the dominant position was occupied by gobies of experimental groups. It should be noted that among the animals of the experimental groups the leading place was occupied by the bulls receiving the feed additive in a dose of 1.0 ml per 10 kg of live weight, that is, the youngest of the III group.


2019 ◽  
Vol 7 (23) ◽  
pp. 4101-4109
Author(s):  
Asmaa Mahmoud Abdeen ◽  
Tarik Essawy ◽  
Saher Sayed Mohammed

BACKGROUND: Sofosbuvir (SOF) was published in 2013 as a part of first-line treatments for hepatitis C virus (HCV); it has activity against all genotypes with extrahepatic adverse effects have recently arisen. AIM: Investigating sofosbuvir-induced alterations in the rat submandibular salivary gland (SMSG). METHODS: A group of 80 adult albino rats weighing about ± 150 gm were used in the experiment. The rats were divided into 3 groups: Group I (control group) received distilled water, Group II (experimental group) divided into 2 subgroups and received SOF 40 mg/kg/day dissolved in distilled water for 1 and 3 months and Group III (recovery group) allowed for 1 month of recovery after SOF withdrawal. All animals were sacrificed; the SMSG was dissected, and specimens were examined histologically and ultra-structurally. RESULTS: Compared to Group I, Group II subgroup (1) showed acinar and ductal vacuolisation, discontinuity of the epithelial lining associated with retained secretion and congested blood vessels. These changes were found to be exaggerated in the subgroup (2) accompanied by acinar and ductal shrinkage, interstitial oedema, haemorrhage, chronic inflammatory cells infiltration and loss of gland compactness. Amelioration of the histological changes was detected in Group III after SOF withdrawal. The ultrastructural examination confirmed the histological results. CONCLUSION: SOF had induced apparent alterations in the structure and ultrastructure of SMSG. The SOF-induced alterations were time-dependent, attributed mainly to mitochondrial toxicity and partially ameliorated by its withdrawal.  


Author(s):  
Kintu Patel ◽  
Bhagyabhumi Patel ◽  
Alkesh Patel ◽  
Samir Shah

In present study, we evaluated the antiulcer activity of the herbal preparation of Caesalpinia crista in rat models.  Experimental animals were divided into four groups. Rats of group I (disease control) treated with normal saline only, group II (standard group) treated with Omeprazole (2 mg/ kg; p.o.), group III and IV served as test groups and were treated with Caesalpinia crista extract (CE) in the dose of 250 mg/ kg and 500 mg/ kg orally respectively. Peptic ulcer was induced by ligating the pyloric portion of rat stomach and was done 45 min after the respective treatment. After 4 hour of pylorus ligation, rats were sacrificed. Parameters like ulcer index, percent ulcer protection, total and free acidity were estimated for evaluation of anti-ulcer activity. Histopathological evaluation was also performed. The aqueous extract of Caesalpinia crista seeds reduced the volume of gastric juice, free acidity, total acidity and ulcer index. It increased the pH of the gastric acid. Histopathology of the rat stomach revealed the presence of lesions and infiltration of inflammatory cells in control group. Moreover, animals treated with test drug and standard drug did not reveal any microscopic lesions. These findings suggest that Caesalpinia crista seeds may have anti-secretory and anti-ulcer activity and may be helpful for ulcer therapy. 


2020 ◽  
Vol 6 (2) ◽  
pp. 286
Author(s):  
Joni Tandi ◽  
Dela Erni Paerunan ◽  
Nurifa Nurifa ◽  
Yunlis Silintowe Kenta ◽  
Sri Mulyani

This study aims to determine the potential for glucose levels in the blood of ethanol extracts of the begonias (Begonia Sp.), the effect of the extract on the pancreatic histopathology of white rats (Rattus norvegicus) which was induced by streptozotocin, and the effective dose begonias’ extract leaves on the histopathological descriptions of pancreatic male white rats. This study was an experimental laboratory where the research group consisted of group I (the control group) as a normal control was given a standard feed, group II (negative control) was given a 0.5% Na CMC suspension, group III (positive control) was orally given glibenclamide and experimental groups IV, V, and VI (doses 50, 100, and 200 mg/kg BW). Were given ethanol extract of begonias leaves. The results showed that the ethanol extract of the begonias (Begonia Sp.) contained secondary metabolite compounds, namely flavonoids, alkaloids, saponins, polyphenols, and tannins. Ethanol extract of begonias leaves at adose of 50 mg/kg BW was an affective dose in reducing blood glucoselevels by an avarage of 101,8 mg/dL) and a dose of 50 mg/kg BW can affect the histopathological picture of the pancreas in STZ induuced rats with an average damage value of 2 from a maximum of 4.


2018 ◽  
Vol 74 (1) ◽  
pp. 6012-2018
Author(s):  
AMIRABBAS MOUSAVI ◽  
GHOLAMREZA ABEDI ◽  
ALIREZA JAHANDIDEH ◽  
SAEED HESARAKI

This study was conducted to determine the quality of anesthesia by evaluating the clinical effects of midazolam-propofol and metamizole-propofol on domestic pigeons. The aim of the study was to choose an appropriate injectable anesthetic protocol by utilizing midazolam and metamizole as pre-anesthetic drugs in order to reduce the dose of propofol and decrease side effects. Eighteen male pigeons weighing 304.2±32.3g (mean±SD) were randomly allocated to three groups of six pigeons each. Midazolam (6 mg/kg/IM) and metamizole (500 mg/kg/IM) were used five minutes prior to administration of propofol (8 mg/kg/IV) in groups I and II, respectively, while the birds in group III (control group) received metamizole (500 mg/kg/IM). Five minutes after the last injection, clinical parameters of various systems were evaluated. Nervous system parameters declined in groups I and II, while reflexes could still be elicited in group III (P<0.05). There were no significant changes in the heart rate, dysrhythmia, or gastrointestinal parameters in the three groups (P>0.05). The birds in group II showed reduced jaw and limb muscle tone (P<0.05). Corneal and palpebral reflexes were absent in groups I and II (P<0.05). Both respiratory depth and pattern were irregular in group I, while all the parameters were normal in group II (P<0.05). The respiratory rate decreased in all groups after injection, although this reduction was significant only in group I (P<0.05). In conclusion, the combination of metamizole-propofol is safer and more compatible with the physiology of pigeons and can be used for short operations, especially when inhalation agents are contraindicated..


2011 ◽  
Vol 8 (2) ◽  
pp. 99
Author(s):  
Fitri Ardiani ◽  
Wiryatun Lestariana ◽  
Emy Huriyati

Background: Diabetes Mellitus (DM) is a chronic metabolic disorder as indicated by hiperglycemia caused by insulin defciency produced by pancreatic islet ß cell which triggers metabolic disorder of carbohydrate, protein and fat, and tends to cause complications. One of the complications is liver damage caused by DM which is probably due to lipid peroxydation, subsequent to free radical production. The common biochemistry signs of liver damage are increasing activities of some enzym such as transaminase pyruvat glutamate serum in the blood. Ruellia tuberosa L is a traditional plant and used by people as diabetic traditional drug. Flavonoid, saponin, zink as antioxidant can decreased of blood glucose in diabetic.Objective: The study aimed to identify the effect of Ruellia tuberosa L extract on glutamate oxaloacetate and glutamate pyruvat transaminases as well as liver histology of diabetic white mice (Rattus norvegicus).Method: The study was pure experimental that used a pre-post test control group design. The study used 30 male Rattus norvegicus. They were divided into 5 groups, each group consisting of 6 rats. Group I rats served as the normal control. Groups II to V consisted of rats with DM induced with alloxan 170 mg/kgBB subcutaneously. The experiment made was as followed. Group I served as control, group II was DM+aquades, group III was DM+Ruellia tuberosa L extract 1.6 mg/ kgBB/day, group IV was DM+Ruellia tuberosa L extract 3.2 mg/kgBB/day, and group V was DM+Ruellia tuberosa L extract 6.4 mg/kgBB/day. Glutamate oxaloacetate and glutamate pyruvat transaminases was measured before beginning and end of the treatment. The blood was taken from sinus orbitalis. At day 30 (end of the study) the rats were sacrifced for their liver. Data of transaminase oxaloacetate glutamate serum and transaminase pyruvat glutamate serum level were analyzed using one way ANOVA. The result of liver histology was analyzed descriptively.Results: The effect of Ruellia tuberosa L extract within 30 days of study could minimize level of transaminase oxaloacetate glutamate serum and transaminase pyruvat glutamate serum of DM rats. Maximal decreasing in extract 3,2 mg. Histologically there was no damage of the liver.Conclusion: Ruellia tuberose L extract could minimize level of transaminase oxaloacetat glutamate serum and transaminase pyruvat glutamate serum of DM and liver histology is normal.


2021 ◽  
Vol 10 (4) ◽  
pp. 38110414286
Author(s):  
Fernanda Cláudia Miranda Amorim ◽  
Emilia Ângela Loschiavo Arisawa ◽  
Luciana Barros Sant’Anna ◽  
Khetyma Moreira Fonseca ◽  
Davidson Ribeiro Costa ◽  
...  

This preclinical study aimed to evaluate the tissue repair process of burns treated with human amniotic membrane (hAM) patches in rats. Twenty-four rats were subjected to superficial burns of partial thickness, and randomly allocated into two groups: Control and Treated Group, subdivided into two experimental periods of 7th and 14th days. The lesions were evaluated by digitalized images (macroscopy) and by the analysis of histological sections stained in H&E to quantify the number of inflammatory cells and fibroblasts present in the different experimental times (histomorphometry). The histomorphometric analyses were performed blindly. Statistical analysis employed Kolmogorov-Smirnov and Mann Whitney tests, with 95% confidence interval at 5% significance level (p <0.05). Macroscopically, the lesions of Treated group presented a crust formation before Control Group, and there were no signs of infection in both groups. Microscopically, the qualitative analysis showed a faster evolution in the healing process of the Treated groups compared to the Control, with reduction of the inflammatory infiltrate, intense fibroblasts proliferation and better organization of the collagen fibers. The quantitative analysis showed statistically significant results regarding the reduction of inflammatory cells (p<0.0001) at 7th and 14 th day and increased proliferation of fibroblasts at 14th day (p<0.0001) in lesions treated with hAM compared to Control group. The results of this preclinical study demonstrated that the application of hAM patches reduces the inflammatory process and accelerates the onset of the proliferative phase in burn injuries.


2018 ◽  
Vol 56 ◽  
pp. 71-76
Author(s):  
I. I. Murzha ◽  
V. G. Кеbkо ◽  
Yu. P. Polupan ◽  
M. G. Porhun ◽  
L. O. Dedova ◽  
...  

Introduction. In recent years, the production of feed additives of animal origin in Ukraine has declined sharply, and the cost of imported additives is very high. At the same time, in our country, the industry of industrial poultry farming has recently received intensive development, in particular, the cultivation and processing of broiler chickens for meat. At the same time, a significant amount of non-food waste from poultry slaughter in many poultry farms is not processed for feed purposes. Currently, an urgent problem is the organization of processing of non-food waste of poultry for feed purposes at poultry farms of industrial type of cultivation for meat of broiler chickens, primarily from blood and hydrolyzed raw materials. Processing of these wastes for feed purposes will not only give the opportunity to increase the production of high-grade protein feed, but also to significantly improve environmental safety. The purpose of our research is to study the meat productivity, safety, slaughter yield and quality of broiler chicken meat when feeding a combined feed additive from blood and hydrolyzed raw materials. Materials and methods of research. Development of an innovative technological line for the production of a combined feed additive from blood and hydrolyzed raw materials was carried out in LLC "Complex Agromars" (village of Havrylivka, Vyshgorod district of Kyiv region), and research on the effectiveness of feeding a combined feed additive from blood and hydrolyzed raw materials was performed in the production conditions of LLC "Bila Hora" Sniatynskyi district of Ivano-Frankivsk region. Research result. Development of innovative technological line was carried out in cooperation with engineering and technical staff of LLC "Complex Agromars". The innovative technological line is designed and installed using devices and mechanisms of the company "Haarslev Industries". In order to study the effectiveness of feeding a combined blood-feather feed additive for the experiment, four groups of broiler chickens of the Cobb 500 cross were formed, 50 heads in each, according to the following scheme: one group of broiler chickens was taken as a control group, and the next three groups – as research groups. Cultivation of broiler chickens in the experiment was carried out according to the traditional technology of feeding on standard compound feeds adopted in industrial broiler poultry farming, which, depending on the age and growth of chickens, includes 3 phases: "Start" – 14 days, "Growth" – 18 days, "Finish" – 12 days. According to the scheme of experience, the control group of chickens received standard feed without blood-feather feed additive. The difference in feeding of the broiler chickens of the experimental groups was in different levels of feeding with experimental combined feed additive from blood and hydrolyzed feather raw materials, which, depending on the age and growth of chickens, included in the feed of the I group from 4 to 12%, of the II group – from 3 to 10%, of the III group – from 2 to 8%. As a result of the studies it was found that at the end of the experiment at 44-day age the average live weight of broiler chickens of the control group was 2630 g, of the experimental group I – 2885 g, of the group II –2790 g, of the group III – 2680 g. For 44 days of experience the following amounts died: in the control group – 2 chickens, in the experimental group I – 1 chicken, in the group II – no chickens, in the group III – 1 chicken. Slaughter of broiler chickens and meat tasting was carried out in the company "Bilyi Bereh" of Ternopil region at the age of 44 days. It was established that the average slaughter yield of 1 carcass of broiler chicken meat was: in the control group – 1920, in the experimental group I – 2106 g, in the group II – 2037 g, in the group III – 1956 g. The difference in quality of meat of broiler chickens of control and experimental groups by results of tasting was not revealed. Summary: In LLC "Complex Agromars" (village of Havrylivka, Vyshgorod district of Kyiv region) designed and implemented an innovative technological line for production of ecologically safe combined feed additive from blood and hydrolyzed feather raw materials from non-food waste slaughter of broiler chickens when growing for meat. In the course of scientific and economic experiment it was found that the inclusion in the standard traditional feed in different phases of cultivation of broiler chickens for meat of about 4 to 12% of the combined feed additive from blood and hydrolyzed feather raw materials increased their average live weight from 2630g in the control group to 2885 g, and the average slaughter yield of 1 meat carcass – from 1920 g in the control group to 2106 g.


Author(s):  
Bayu Putra ◽  
Rizqi Nur Azizah ◽  
Eka Maryam Nopriyanti

Portulaca oleracea L. is one of the plants empirically used as medicine and contains flavonoid compounds assumed to have an immunomodulatory effect. The research aimed to determine the immunomodulatory effect of ethanol extract of Portulaca oleracea L  by Delayed Type Hypersensitivity parameter. The research used 20 male rats (Rattus norvegicus) divided into 5 groups where each group consisted of 4. Group I as a normal group (without treatment), group II (NaCMC control), group III, IV and V (treatment groups) were given EEHK with doses of 100 mg/kg BW, 200 mg/kg BW, and 400 mg/kg BW doses. The dosage form was orally given for 7 days. On day 3, the rats were induced with SDMD antigen 10% v/v by intraperitonial and intraplantar on day 7. The observations were conducted by measuring the volume change of the rat's feet at the 4th, 24th, and 48th hours using a pletismometer. The research data obtained were statistically processed using Paired Samples T Test, One-Way Anova and Post Hoc LSD tests. The results of the research showed that the ethanol extract of common purslane was effective as an immunomodulator.


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