scholarly journals Uji Toksisitas Subkronik 28 Hari Kombinasi Ekstrak Metanolik Benalu Teh dan Benalu Mangga terhadap Profil Protein pada Tikus Wistar (Rattus norvegicus) Betina

2021 ◽  
Vol 7 (1) ◽  
pp. 9-15
Author(s):  
Umu Intan Kinasih ◽  
Nour Athiroh Abdoes Sjakoer ◽  
Nurul Jadid Mubarakati
Keyword(s):  
Body Weight ◽  
Data Analysis ◽  
Total Protein ◽  
Rattus Norvegicus ◽  
Wistar Rat ◽  
Control Treatment ◽  
Protein Breakdown ◽  
Total Proteins ◽  
Animal Test ◽  
And Control

There are 3 indicators for indicating a protein breakdown in the blood through a total protein, albumin, and globulin stability. A compound capable of acting as antioxidants is needed to stabilize the protein's condition. This research is aimed at identifying the toxic effects of extract’s combination from tea parasite and mango parasite on the conditions of blood’s protein by giving EMBTBM constantly for 28 days. The data analysis used ANOVA one-way by SPSS version 17.0. As for the animal test used was a female Wistar rat of 20 with four treatments are control, treatment 1 with a dose of 250mg/Kg body weight (BW). Treatment 2 with a dose of 500mg/Kg body weight (BW) and treatment 3 with a dose of 1000mg/Kg body weight (BW). The result of this research shows that there is no difference between treatment and control over the results of a total protein and albumin. But, at the result of globulin, there is a difference between treatment and control. Therefore, EMBTBM is safe for profile protein and does not cause toxicity because protein is stable and not over-reducing.   Keywords:Subchronic,Total Proteins, Albumine, Globuline, Toxicity

scholarly journals Kidney Function Test of Female Wistar Rat (Rattus norvegicus Berkenhout, 1769) of Subchronic Toxicity Test of Arthrospira maxima sp. and Chlorella vulgaris sp.

2019 ◽  
Vol 4 (3) ◽  
pp. 119
Author(s):  
Mulyati Mulyati ◽  
Anita Yuliana ◽  
Slamet Widiyanto
Keyword(s):  
Body Weight ◽  
Kidney Function ◽  
Chlorella Vulgaris ◽  
Rattus Norvegicus ◽  
Toxicity Test ◽  
Wistar Rat ◽  
Control Group ◽  
Histological Structure ◽  
Subchronic Toxicity ◽  
Arthrospira Maxima

Arthrospira maxima and Chlorella vulgaris are contained a high protein and antioxidant levels that can be used as functional foods or supplements to improve health. Furthermore, this material needs to be monitored for safety. The aims of this research were determined the subchronic toxicity test of microalgae A. maxima and C. vulgaris on kidney function. Parameters of this research were creatinine levels, urea levels, kidney index, and histological structure of the kidney glomerular. Besides that, the progression of body weight was observed. Five teen female Wistar rats (Rattus norvegicus Berkenhout,1769) divided into three groups randomly were one control group and two treatment groups using A. maxima and C. vulgaris in the same dose were 2500 mg/kg of body weight. Subchronic toxicity test was conducted by oral gavage every day during 90 days — the measurement of creatinine levels and urea levels on the 30th, 60th, and 90th day. Kidney index and glomerular histology of rat’s kidney was prepared after necropsy at the end of this research. Base on the results, it can be concluded that consume of Arthrospira maxima, and Chlorella vulgaris at 2500 mg/kg of body weight increased creatinine and urea levels. Bodyweight, kidney index, and pathological glomerular cells of histological kidney were still in normal value.

scholarly journals Comparing difference in mean total protein, albumin and globulin based on severity of rhesus isoimmunization: a prospective study

2020 ◽  
Vol 9 (7) ◽  
pp. 3042
Author(s):  
Vikas Yadav ◽  
Dipika Deka ◽  
Aparna Sharma ◽  
Vatsla Dadhwal
Keyword(s):  
Total Protein ◽  
Fetal Outcome ◽  
Control Group ◽  
Fetal Blood ◽  
Total Proteins ◽  
Intrauterine Transfusion ◽  
Rh Incompatibility ◽  
The Difference ◽  
A Prospective Study ◽  
And Control

Background: Maternal RBC alloimmunization results from exposure and response to a foreign RBC antigen. Transplacental fetal to maternal hemorrhage is the most common cause of alloimmunization. Rh incompatibility can lead to either fetuses with hydropic features or non-hydropic. The precise mechanism leading to the development of hydrops is uncertain. Biochemical markers have the potential to be used to assess the severity of problem. But of the mechanisms proposed none have been able to totally explain the phenomenon or predict the prognosis. Objective of this study wads to compare the difference in mean total protein, albumin and globulin bases on severity of isoimmunization and comparing it with normal controls.Methods: A Total of 40 pregnant patients were enrolled which included 10 hydropic fetuses of Rh isoimmunised mothers, 10 non hydropic fetuses of Rh isoimmunized mothers. Control group included 18 Rh positive women without any fetal complication and 2 fetuses in women undergoing cordocentesis. Blood sampling was done at time of intrauterine transfusion and sent for estimation of total proteins, albumin, globulin in fetal blood. Pregnancies were followed up till delivery and fetal outcome noted.Results: Mean total protein, albumin and globulin between hydropic, non hydropic group and control group (3.25, 2.17 and 1.18 g/dl) in hydropic, (4.14, 2.70 and 1.44 g/dl) in non hydropic and (4.42, 2.95 and 1.47 g/dl) in control group respectively. Mean total protein, albumin and globulin between mild hydropic (3.43, 2.30 and 2.10 g/dl) and severe hydropic group (2.59, 1.6 and 1.3 g/dl) respectively.Conclusions: There was significantly lower levels of serum total proteins, albumin and globulin in hydropic fetuses as compared to non hydropic fetuses. Thus, hypoproteinemia can be considered a strong marker for development of hydrops in Rh isoimmunized fetuses.

scholarly journals Muscular strength decrease in Rattus norvegicus experimentally infected by Toxocara canis

2009 ◽  
Vol 51 (2) ◽  
pp. 73-75 ◽  
Author(s):  
Pedro P. Chieffi ◽  
Renata T.R. Aquino ◽  
Maria A. Paschoalotti ◽  
Manoel Carlos S.A. Ribeiro ◽  
Antonia G. Nasello
Keyword(s):  
Body Weight ◽  
Rattus Norvegicus ◽  
Wistar Rats ◽  
Muscular Strength ◽  
Toxocara Canis ◽  
The Body ◽  
And Control

The muscular strength of experimental infected Rattus norvegicus with 3rd. stage Toxocara canis larvae was investigated. Fifty Wistar rats, divided in three groups (G1 - 20 rats infected by 300 eggs of T. canis; G2 - 20 rats infected by 2,000 eggs of T. canis and G3 - 10 rats without infection) had been used. Ten and 30 days after infection the muscular strength in the fore-feet of the rats was checked; at the same time, the body weight was determined. No significative differences in the body weight were noted among the infected and control rats in both occasions. Otherwise, an impairment on the muscular strength was observed in rats infected with T. canis 30 days after inoculation.

scholarly journals Pengaruh Pemberian Teh Kombucha Konsentrasi 50% dengan Waktu Fermentasi yang Berbeda pada Struktur Histologi Hepar Tikus Wistar (Rattus norvegicus) Jantan

2021 ◽  
Vol 23 (1) ◽  
pp. 51-56
Author(s):  
Sri Isdadiyanto ◽  
Silvana Tana
Keyword(s):  
Data Analysis ◽  
Rattus Norvegicus ◽  
Cell Damage ◽  
Control Treatment ◽  
Histological Structure ◽  
White Rats ◽  
Randomized Design ◽  
Kombucha Tea ◽  
Significant Difference ◽  
Completely Randomized Design

This study was conducted to determine the effect of 6 kombucha fermented tea (P1), 9 (P2) and 12 (P3) fermentation days at a concentration of 50% on the liver histological structure and hepatocyte diameter. This study used 16 male white rats consisting of 4 treatments and 4 replications. The treatment of this study consisted of P0: control treatment; P1, P2 and P3 respectively were given 6, 9 and 12 days fermented kombucha tea at a concentration of 50%. This research uses a completely randomized design. The variable measured in this study was the diameter of hepatocytes in test animals. The data obtained were analyzed using Analysis of Variance (ANOVA) at a 95% confidence level and if there were real differences, followed by Duncan's test. The results of data analysis showed that kombucha tea fermented 6, 9 and 12 days with a 50% concentration did not have a significant difference in hepatocyte diameter variables. The histological structure of the liver looks normal and shows no sign of cell damage. It can be concluded that kombucha tea at a concentration of 50% is safe for consumption by mammals because it does not cause liver damage.

scholarly journals Pembasmi Obligat Hama Tikus Dari Ampas Teh Terhadap Pertumbuhan Dan Tingkat Mortalitas Rattus norvegicus

Elkawnie ◽  
2019 ◽  
Vol 5 (2) ◽  
pp. 121
Author(s):  
Hadita Amila ◽  
Nurul Afifah ◽  
Ramaidhani Ramaidhani ◽  
Fitriani Fitriani
Keyword(s):  
Body Weight ◽  
Data Analysis ◽  
Rattus Norvegicus ◽  
Crop Yields ◽  
Food Crops ◽  
Considerable Decrease ◽  
Significance Level ◽  
Rat Growth

Rat as one of the important pests that attackcrops in Indonesia. This pest attacks food crops in all phases of growth so that it can cause a considerable decrease in crop yields. So it is necessary to eradicate rat pests by using politicians from tea pulp. The purpose of this study was to determine how the influence of political use on rat growth and mortality. The design used is RAL which consists of 5 treatments, namely 0 ml/L, 3 ml/L, 6 ml/L, 9 ml/L, and 12 ml/L. The parameters observed were: mortality, weight, and appetite for mice. Data analysis using ANOVA at 5% significance level and continued with DMRT. The results showed that there was an influence of obligat rat pest use on body weight, appetite, and mortality of mice, obligat rat pest who effectively increased mortality reduced rat body weight, rat appetite and rat mortality in the treatment of 12 ml/L.

scholarly journals The Metabolic Effects of Consumption of Yellow Cassava (Manihot esculenta Crantz) on Some Biochemical Parameters in Experimental Rats

2015 ◽  
Vol 34 (6) ◽  
pp. 559-564
Author(s):  
Nelson Udeme ◽  
Polycarp Okafor ◽  
Chinedum Eleazu
Keyword(s):  
Body Weight ◽  
Serum Glucose ◽  
Metabolic Effects ◽  
Control Group ◽  
Albino Rats ◽  
Free Cyanide ◽  
Control Groups ◽  
Total Proteins ◽  
Manihot Esculenta Crantz ◽  
And Control

The metabolism of yellow cassava (variety TMS 01/1368) was investigated in male albino rats fed a diet containing yellow cassava for 7 to 28 days. There were significant increases ( P < 0.05) in total and free cyanide and thiocyanate in the sera and urine samples of the experimental rats compared with the control, significant increases ( P < 0.05) in serum glucose, alanine aminotransaminase, aspartate aminotransaminase, and alkaline phosphatase levels of the experimental rats compared with the control, significant decreases ( P < 0.05) in serum albumin of the experimental rats compared with the control, but no significant differences ( P > 0.05) in the serum total proteins of the experimental rats compared with the control. The experimental rats treated for 7, 14, 21, or 28 days exhibited body weight decreases of 5.11%, 11.10%, 19.16%, and 24.18%, respectively, whereas the control group showed 9.17% gain in body weight. Total and free cyanide concentrations were detected in the liver, kidney, and heart of most of the rats in both the experimental and control groups, except for free cyanide in the control group that was not detected. Metabolism of the yellow cassava variety in experimental rats was capable of exposing the animals to cyanide, underscoring the need for its proper processing before consumption by humans.

Measurements, Data Analysis and Control Methods in Wastewater Treatment Plants–State of the Art and Future Trends

1989 ◽  
Vol 21 (10-11) ◽  
pp. 1333-1345 ◽  
Author(s):  
Gustaf Olsson ◽  
Bengt Andersson ◽  
Bengt Göran Hellstrom ◽  
Hans Holmström ◽  
Lars Gunnar Reinius ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Data Analysis ◽  
Dynamic Models ◽  
Model Building ◽  
Measurement Data ◽  
Treatment Plant ◽  
Relevant Information ◽  
Control Treatment ◽  
Control Methods ◽  
And Control

This paper is a summary of a committee working for the Swedish Water and Wastewater Works Association (VAV). The purpose of the report is to present the possibilities today to measure, present and analyze data and control treatment plants. The typical audience is the operator, the process engineer, or the consulting engineer. The methods presented are all known from different disciplines, but are here presented in a form that connects the methods to wastewater treatment operation. Unlike any manual of practice the report is not a concensus report of current practice-Rather it is an attempt to show the potential of modern methods for data analysis and control. This will help the potential equipment or computer buyer to specify relevant demands for the system. The fact that any wastewater treatment plant is highly dynamic has to be reflected both in measurements and in control. The report discusses relevant sampling times for different measurements, both from the inherent dynamics and from the variability of the disturbances. Current design practice is almost always based on steady state analysis, and disturbances are too often controlled by larger tank volumes rather than relevant control actions. In order to obtain relevant data analysis the purpose of the measurement has to be clearly stated. Interesting and relevant measurement variables are listed. Moreover, a short survey of existing instrumentation and its status is presented. The transfer of data from the primary sensor to the computer has to be carefully designed. Once the data is in the computer, the data structure must be specified. The different compromizes between storage capacity, data formats and other relevant information are discussed. Simple measurement handling is described before statistical analysis is discussed. Numerous examples demonstrate the results. Some methods for parameter estimation and model building from measurement data are discussed, particularly with the purpose to make the methods available for on-line use. It is shown how estimated models can be used for the operation of plants. Different control methods are discussed. The basic kind of local control to keep the plant running is first mentioned, but more emphasis is laid on plant quality control, like dissolved oxygen, return sludge and waste sludge control. Dynamic models offer interesting possibilities for plant simulation, and simulators are being developed, that can support the operator with further predictive information. Some future possibilities of knowledge-based systems for process diagnosis are further discussed. They offer new possibilities to use natural language for systematic error analysis and diagnostic searches.

Overtraining of aerobic physical exercise reduce rats (Rattus norvegicus) memory

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
Ni Made Ridla Parwata
Keyword(s):  
Body Weight ◽  
Physical Exercise ◽  
Rattus Norvegicus ◽  
Research Method ◽  
Male Rat ◽  
Control Group ◽  
Adult Male Rat ◽  
The Subject ◽  
Heavy Training

Overtraining syndrome is a decrease in physical capacity, emotions and immunity due to training that is too often without adequate periods of rest. Overtraining is often experienced by athletes who daily undergo heavy training with short break periods. This research aims to look at the effect of overtraining aerobic physical exercise on memory in mice. The research method was experimental in vivo with the subject of adult male rat (Rattus Norvegicus) Winstar strain aged 8-10 weeks, body weight 200-250 gr. Divided into three groups, namely the control group, aerobic group and overtraining group. The results of memory tests with water E Maze showed an increase in the duration of travel time and the number of animal errors made by the overtraining group (p = 0.003). This study concludes that overtraining aerobic physical exercise can reduce memory in rat hippocampus.

Drought stress and osmo-priming effects on physiological and biochemical characteristics of Ocimum basilicum

2016 ◽  
Vol 6 (1) ◽  
pp. 905-913
Author(s):  
Bahram Majd Nassiry ◽  
Neda Mohammadi
Keyword(s):  
Drought Stress ◽  
Total Protein ◽  
Catalase Activity ◽  
Seedling Emergence ◽  
Seed Priming ◽  
Ocimum Basilicum ◽  
Field Development ◽  
Physiological And Biochemical Characteristics ◽  
Germination Characteristics ◽  
And Control

    One of the effects of reducing water content on soil is reduction of growth and development of seedlings and variation of field development. Seed priming technique has been known as a challenge to improve germination and seedling emergence under different environmental stresses. The objectives of this research were to evaluate the effects of osmo-priming on germination characteristics and changes of proline, protein and catalase activity of Ocimum basilicum seeds. Results showed that drought stress reduced the germination characteristics and drought stress in -8 bar was the critical stress.  Priming treatments were include KNO3, PEG and NaCl by 0, -4 and -8 bar concentrations. The seeds were primed with those materials for 8 and 16 hours. The highest germination characteristics were obtained from nitrate potassium in -8 bar for 16 hours priming. Therefore the best seed treatment under drought stress during germination was obtained from the osmo-primed with -8 bar nitrate potassium for 16 hours. The drought stress increased proline and catalase activity but reduced total protein. Priming treatment increases proline, total protein and catalase activity under drought and control conditions. It is concluded that priming results in improvement in germination components of Ocimum basilicum in drought stress conditions and increases the resistance to drought stress with improvement of proline, protein and catalase activity in germination phase.

scholarly journals Multivariate statistical data analysis of cell‐free protein synthesis toward monitoring and control

AIChE Journal ◽  
2021 ◽  
Author(s):  
Carlos A. Duran‐Villalobos ◽  
Olotu Ogonah ◽  
Beatrice Melinek ◽  
Daniel G. Bracewell ◽  
Trevor Hallam ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Data Analysis ◽  
Statistical Data ◽  
Monitoring And Control ◽  
Statistical Data Analysis ◽  
Multivariate Statistical ◽  
Free Protein ◽  
And Control ◽  
Cell Free Protein Synthesis
Sign in / Sign up

Export Citation Format

Share Document