scholarly journals D-Galactose Induced Changes in Enzymatic Antioxidant Status in Rats of Different Ages

2015 ◽  
pp. 61-70 ◽  
Author(s):  
N. HADZI-PETRUSHEV ◽  
V. STOJKOVSKI ◽  
D. MITROV ◽  
M. MLADENOV
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Status ◽  
Natural Aging ◽  
Antioxidant Enzyme Activities ◽  
Enzymatic Antioxidant ◽  
The Third ◽  
Old Rats ◽  
Induced Changes ◽  
Gpx Activity

Considering the preexisting influence of the process of natural aging on antioxidant enzymes activity and the level of lipid peroxidation, the age of the rats at which D-galactose (D-gal) treatment is started could strongly impact the development of D-gal induced senescence. To evaluate this, we subjected 1, 3 and 15 months old rats to D-gal treatment in parallel with having appropriate placebos (0.9 % saline). Our results showed elevated glutathione peroxidase (GPx) activity and no significant changes in superoxide dismutase (SOD), catalase (CAT) and glutathione reductase (GR) activity or malondialdehyde (MDA) levels in relation to natural aging. In mature and aged senescent livers we observed positive correlation between increased ratio R=SOD/(GPx+CAT) and increased MDA concentration. MDA levels seemed to correlate positively with the age of the animals at which D-gal treatment had started. In the case of 3 and 15 months old rats there was D-gal induced decrease in SOD and GR activity, but this effect of the treatment was not observed in 1 month old rats. Our results imply that the changes in the antioxidant enzyme activities are not only under the influence of the D-gal overload, but also depend on the developmental stage of the rats. According to our results, with regard to enzymatic antioxidant capacity and the level of lipid peroxidation, the best age for induction of senescence is somewhere after the third month.

scholarly journals PROCESSES OF LIPID PEROXIDATION AND SYSTEM OF ANTIOXIDANT PROTECTION IN THE BLOOD OF THE UMBILICAL CORD OF INFANTS AT CYTOMEGALOVIRUS INFECTION IN PREGNANCY

2018 ◽  
Vol 1 (70) ◽  
pp. 74-77
Author(s):  
Николай Дорофиенко ◽  
Nikolay Dorofienko
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Arachidonic Acid ◽  
Umbilical Cord ◽  
Cytomegalovirus Infection ◽  
Antioxidant Status ◽  
Superoxide Dismutase Activity ◽  
Antioxidant Protection ◽  
Third Trimester ◽  
The Third

The aim of the work is to study the activity of lipoperoxidation processes and antioxidant protection system in the umbilical cord blood of newborns from CMV-seropositive women with exacerbation of infection in the third trimester of pregnancy. Lipid peroxidation activity and antioxidant status in 30 newborns from CMV-seropositive women with exacerbation of infection in the third trimester of pregnancy and in 25 from CMV-seronegative women were studied. Erythrocytes were used to determine the products of oxidative modification of lipids; antioxidant status was evaluated in the blood plasma of the umbilical vein. The content of TBK-active products was determined by fluorometric method, lysophosphatidylcholine by thin-layer chromatography, arachidonic acid by gas-liquid chromatography. Antioxidant status was assessed by α-tocopherol content and superoxide dismutase activity. Registration of optical densities and fluorescence was carried out using a spectrofluorometer. The study found an increase in the content of red blood cells of the umbilical cord of newborns from CMV-seropositive women with an exacerbation of infection in the third trimester of pregnancy of TAC-active products in 2.1 times (p˂0.001), of lysophosphatidylcholine in 1.9 times (p˂0.001) and of arachidonic acid in 1.5 times (p˂0.001). The antioxidant status of umbilical cord blood was characterized by 1.5 times decrease in α-tocopherol (p<0.01) and 1.3 times decrease in superoxide dismutase activity (p˂0.001) compared with similar indices in the group of newborns from CMV-seronegative women. The results of the study show that the exacerbation of cytomegalovirus infection in the third trimester of pregnancy is accompanied by the development of oxidative stress and tension in the system of antioxidant protection in the blood of the umbilical cord of newborns due to increased consumption of α-tocopherol and suppression of superoxide dismutase activity.

The Impact of Concomitant Chronic Pancreatitis on Prooxidant-antioxidant Status and Other Conditions in Osteoarthritis

2016 ◽  
pp. 75-78
Author(s):  
Liliia Babynets ◽  
Tetiana Maevska
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Chronic Pancreatitis ◽  
Functional Capacity ◽  
Antioxidant Status ◽  
Activation Parameters ◽  
Tissue Destruction ◽  
The Impact ◽  
Stress Accumulation

The study proved that patients with combined progress of osteoarthritis and chronic pancreatitis have reliable top-level activation of lipid peroxidation in terms of malonyc aldehyde and tissue destruction in terms of oxyproline, weakening of the antioxidant level (in terms of superoxide dismutase and SH-groups) and activation parameters of catalase and ceruloplasmin (p<0,05). The authentic predictority of patients biological age, duration of combined clinical courses, the functional capacity of the pancreas in terms of fecal α-elastase, structural state by ultrasound criteria for progression effects of oxidative stress, accumulation oxyproline activation parameters catalase and ceruloplasmin, which statistically was reflected by the presence of mainly moderate of significant correlations between these groups of indicators have been identified.

Antioxidant status and lipid peroxidation after short-term maximal exercise in trained and untrained humans

1997 ◽  
Vol 272 (4) ◽  
pp. R1258-R1263 ◽  
Author(s):  
N. Ortenblad ◽  
K. Madsen ◽  
M. S. Djurhuus
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Enzyme ◽  
Antioxidant Status ◽  
Antioxidant Activities ◽  
Antioxidant Enzyme Activities ◽  
Muscle Enzyme ◽  
Jump Exercise ◽  
Jump Test ◽  
Coupled Enzymes ◽  
Resting Muscle

The purpose of this study was to measure resting muscle and blood antioxidant status in untrained (n = 8) and jump-trained (n = 8) humans and to evaluate free radical-mediated muscle damage after a strenuous jump test consisting of six bouts of 30-s continuous jumping separated by 2 min of rest. Resting muscle antioxidant activities [superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione reductase (GR), and manganese SOD] were significantly higher in jump-trained compared with untrained subjects. Blood antioxidant enzyme activities and muscle catalase, however, were not different between the two groups. Creatine kinase activities increased significantly (P < 0.0001) after the jump test in untrained individuals, but remained unchanged in the jump trained. Plasma and muscle malonaldehyde (MDA) after the jump test were not significantly different from rest. These data suggest that jump training is associated with elevated activities of SOD and the coupled enzymes GPX and GR in muscle tissue, but other antioxidants remain unchanged. High-intensity jump exercise induces muscle enzyme leakage in untrained humans, but muscle lipid peroxidation, measured as changes in MDA, was not different in the two groups despite the varied muscle antioxidant enzyme levels.

Patients with Sickle Cell Disease have Reduced Blood Antioxidant Protection

2008 ◽  
Vol 78 (3) ◽  
pp. 139-147 ◽  
Author(s):  
Ren ◽  
Ghebremeskel ◽  
Okpala ◽  
Lee ◽  
Ibegbulam ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Superoxide Dismutase ◽  
Sickle Cell ◽  
Antioxidant Status ◽  
Mononuclear Cells ◽  
Red Cell ◽  
Omega 3 ◽  
Sod Activity ◽  
Hemoglobin C ◽  
Gpx Activity

In previous studies, we found that homozygous sickle cell (HbSS) patients, compared with their healthy (HbAA) counterparts, had reduced levels of the omega-3 fatty acids, eicosapentaenoic (EPA) and docosahexaenoic (DHA) acids, in red cells, platelets, and mononuclear cells. These differences were not due to lower intake of the two fatty acids. We have investigated whether reduced antioxidant status in the patients could help explain the observed phenomenon. Blood specimens previously obtained for fatty acid study from Nigerian (26 HbSS and 30 HbAA) and British (30 HbSS, 9 sickle cell-hemoglobin C/HbSC, and 15 HbAA) subjects were analyzed for antioxidant status. The Nigerian HbSS patients compared with the controls had lower plasma retinol, α-tocopherol, and β-carotene concentrations (p < 0.005) and reduced activity of red cell Cu/Zn-superoxide dismutase (Cu/Zn-SOD) (p < 0.05). Similarly, the British HbSS group had reduced concentrations of plasma α-tocopherol (p < 0.005), and activities of red cell Cu/Zn-superoxide dismutase (p < 0.05) and Se-glutathione peroxidase (Se-GPx) (p < 0.005) than the controls. In addition, the British patients in comparison with those who had HbSC, a mild form of the disease, had lower α-tocopherol than that of the HbAA controls (p < 0.005). In the British sickle cell patients, there was a positive correlation between red cell ethanolamine phosphoglyceride (EPG) DHA and Cu/Zn-SOD activity (r = 0.700, p < 0.05), choline phosphoglyceride (CPG) DHA and Se-GPx activity (r = 0.605, p < 0.05), and CPG EPA and Se-GPx activity (r = 0.558, p > 0.05). Similarly, the percent DHA in red cell EPG was positively related with the activity of Se-GPx in the patients with HbSC (r = 0.674, p < 0.05). These findings suggest that the lower levels of membrane EPA and DHA in blood cells of the HbSS patients could be due to peroxidation resulting from a compromised antioxidant competence.

Effect of Sodium Metabisulfite on Oxidative Stress and Lipid Peroxidation Biomarkers

2020 ◽  
Vol 16 (1) ◽  
pp. 114-117
Author(s):  
Shahnaz Shekarforoush ◽  
Parisa Ebrahimi ◽  
Akbar Afkhami Fathabad ◽  
Elaheh Farzanfar
Keyword(s):  
Lipid Peroxidation ◽  
Antioxidant Status ◽  
Treated Group ◽  
Control Group ◽  
Antioxidant Enzyme Activities ◽  
Sodium Metabisulfite ◽  
Male Wistar Rats ◽  
Pharmaceutical Agents ◽  
Cellular Components ◽  
Dose Dependent

Background: Sulfites are widely used as preservatives in the foods and pharmaceutical agents. It has been demonstrated that sulfites can react with a variety of cellular components and cause toxicity. Objective: The present study was designed to investigate the effects of ingested sodium metabisulfite (SMB) on serum antioxidant status in rats. Methods: Thirty-two male Wistar rats were randomly divided into control and treated groups. Treated groups received 10, 100, and 260 mg/kg body weight of SMB for 28 days. After 28 days, serum was assayed for measuring superoxide dismtase (SOD), glutathione peroxidase (GPx), glutathione reductase (GR), catalase (CAT) activities, glutathion (GSH) level and lipid peroxidation. Results: The results showed that the activities of GPx, GR, CAT and GSH levels were significantly decreased in 100 and 260 mg/kg SMB treated rats, while malondialdehyde (MDA) level was significantly increased in 260 mg/kg treated group when compared with the control group. Conclusion: It is concluded that SMB administration as dose-dependent is associated with decreased serum antioxidant enzyme activities and increased lipid peroxidation.

scholarly journals Comparison between Organic and Conventional Spelt and Wheatgrass juice

2019 ◽  
Vol 64 (4) ◽  
pp. 514-522
Author(s):  
Boris M. Popović ◽  
Dubravka M. Štajner ◽  
Ružica Z. Ždero-Pavlović ◽  
Marija I. Bodroža-Solarov ◽  
Vladan M. Ugrenović ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Status ◽  
Antioxidant Properties ◽  
Antioxidant Power ◽  
Conventional Production ◽  
Ferric Reducing Antioxidant Power ◽  
Capacity Index ◽  
Better Than

This work is aimed to compare antioxidant and scavenger properties of conventionally and organically produced speltgrass. Moreover, the antioxidant status of spelt was compared with wheatgrass. Three spelt varieties were analyzed:Nirvana, Ebners–Rotkorn variety and Eco-10. Antioxidant enzymes, superoxide-dismutase and guiacol-peroxidase, were determined as well as ferric reducing antioxidant power and DPPH-antiradical power. Total soluble proteins and total phenols were determined and also lipid peroxidation as a parameter of oxidative stress. The organically produced spelt was exposed to the higher levels of oxidative stress than conventionally produced spelt, and also DPPH antiradical power was induced by conventional production, which is also demonstrated using the relative antioxidant capacity index. The best antioxidant properties, better than wheat, showed organically produced spelt genotype Ebners–Rotkorn which is determined by the highest phenol content, superoxide-dismutase activity, DPPH antiradical power and ferric reduced antioxidant power and consequently with the lowest lipid peroxidation intensity.

Methylnitrosourea, dimethylbenzanthracene and benzoapyrene differentially affect redox pathways, apoptosis and immunity in zebrafish

2020 ◽  
Vol 39 (7) ◽  
pp. 920-929
Author(s):  
G Eğimezer ◽  
ÜV Üstündağ ◽  
PS Ateş ◽  
İ Ünal ◽  
FD Üstündağ ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Antioxidant Status ◽  
Early Embryogenesis ◽  
Mrna Levels ◽  
Glutathione S Transferase ◽  
Immunity Genes ◽  
Genotoxic Carcinogens ◽  
Different Levels

Cancer continues to be a major cause of mortality globally. Zebrafish present suitable models for studying the mechanisms of genotoxic carcinogens. The aim of this study was to investigate the interaction between oxidant–antioxidant status, apoptosis and immunity in zebrafish that were exposed to three different genotoxic carcinogens methylnitrosourea, dimethylbenzanthracene, benzoapyrene and methylnitrosourea + dimethylbenzanthracene starting from early embryogenesis for 30 days. Lipid peroxidation, nitric oxide levels, superoxide dismutase and glutathione- S-transferase activities and mRNA levels of apoptosis genes p53, bax, casp3a, casp2 and immunity genes fas, tnfα and ifnγ1 were evaluated. The disruption of the oxidant–antioxidant balance accompanied by altered expressions of apoptotic and immunity related genes were observed in different levels according to the carcinogen applied. Noteworthy, ifnγ expressions decreased in all carcinogen-exposed groups. Our results will provide basic data for further carcinogenesis research in zebrafish models.

scholarly journals Chelated micronutrient fertilizers as effective antioxidants applied for foliar plant treatment

2020 ◽  
Vol 17 ◽  
pp. 00057
Author(s):  
Valentina M. Pakhomova ◽  
Anisa I. Daminova
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Trace Elements ◽  
Antioxidant Enzymes ◽  
Antioxidant Status ◽  
Field Experiments ◽  
Antioxidant Effect ◽  
Foliar Treatment ◽  
Iron Manganese ◽  
Plant Treatment

The antioxidant status of spring wheat cells was studied for foliar treatment with a 0.1 % solution of copper, molybdenum-containing chelated micronutrient grade ZhUSS-2 in field experiments when cultivating plants on soil with a low content of these trace elements. The activation of the antioxidant enzymes of superoxide dismutase and peroxidase, as well as a decrease in the activity of lipid peroxidation (according to the content of malondialdehyde) under the action of this drug in all phases of plant vegetation was identified. A similar effect of iron-, manganese- and zinc-containing ZhUSS micronutrient fertilizers is analyzed. The conclusion is made about the antioxidant effect of these chelated micronutrient fertilizers.

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