scholarly journals IN-VITRO SCREENING OF DIFFERENT TOMATO GENOTYPES AGAINST PEG INDUCED WATER STRESS

2019 ◽  
Vol 4 (3) ◽  
pp. 15
Author(s):  
Muhammad Saqib Naveed ◽  
Ayesha Manzoor ◽  
Ahsan Javed ◽  
Muhammad Azeem Tariq
Keyword(s):  
Water Stress ◽  
Growth Parameters ◽  
Germination Percentage ◽  
Shoot Length ◽  
Growth Stages ◽  
Randomized Design ◽  
Seedling Biomass ◽  
Root And Shoot Length ◽  
Tomato Genotypes

Tomato (Lycopersicum esculentum L.) is one of the most economically important plants in the Solanaceae family. Water scarcity is one of the major climatic constraints which is affecting all crops including tomato in the world. Selection of water stress tolerant cultivars is an important strategy to overcome this problem. Therefore the present research was conducted to determine the effect PEG induced water stress on germination percentage and early growth stages of tomato seedlings. Fifteen different tomato genotypes were screened under in-vitro conditions using two different concentrations of PEG (2% and 4%). The experiment was arranged in a completely randomized design (CRD) with three replications. Parameters like germination percentage (%), root length (cm), shoot length (cm) and seedling biomass (g) were recorded during the experiment. Results indicated that PEG induced water stress significantly reduced germination percentage (%) and other growth parameters in drought susceptible tomato genotypes (RUS-1, Rustam, R-2017, Pakit, 17904, Kashmiri, Kiara, Avinash, and K.K.2). Whereas in other genotypes “R. Wonder, Naqeeb, Rio grande, T-4, Patfeeder and Nagina” all parameters increase with an increase in PEG concentration. However among these genotypes, “Patfeeder” exhibited highest germination percentage (100 %) with maximum root and shoot length and seedling biomass at highest PEG concentration 4%. Based on experimental results, “Patfeeder” was considered a drought tolerant genotype due to its better performance in different levels of water stress.

scholarly journals Germination and Biochemical Responses to Alkalinity Stress in Two Sesame Cultivars

2016 ◽  
Vol 73 (1) ◽  
pp. 79-86
Author(s):  
Batool Mahdavi
Keyword(s):  
Germination Rate ◽  
Soluble Sugars ◽  
Dry Weight ◽  
Germination Percentage ◽  
Shoot Length ◽  
Percentage Germination ◽  
Total Soluble Sugars ◽  
Randomized Design ◽  
Root And Shoot Length ◽  
Completely Randomized Design

Abstract In this study, the effect of different alkaline concentrations (0, 10, 20, 30, 40, 50, 60 mM) on germination and biochemical characteristics of the two sesame (Sesamum indicum L.) cultivares (Dashtestan and GL-13) which are registered cultivars of Iran were investigated. The experiment was carried out in a completely randomized design with three replications. Results showed that, germination percentage, germination rate, shoot length and dry weight, root length and dry weight and K+ content decreased, whereas, malondialdehyde (MDA), proline, total soluble sugars and Na+ contents increased with increasing alkalinity stress. GL-13 cultivar had the least root and shoot length, proline and K+ content than Dashtestan.

scholarly journals IDENTIFICATION OF DROUGHT TOLERANT WHEAT GENOTYPES UNDER WATER DEFICIT CONDITIONS

2016 ◽  
Vol 4 (2) ◽  
pp. 206-214 ◽  
Author(s):  
Zaid Chachar ◽  
N. A. Chachar ◽  
Q.I. Chachar ◽  
S.M Mujtaba ◽  
G.A Chachar ◽  
...  
Keyword(s):  
Water Stress ◽  
Seed Germination ◽  
Osmotic Stress ◽  
Root Length ◽  
Dry Weight ◽  
Shoot Length ◽  
Wheat Genotypes ◽  
Maximum Reduction ◽  
Drought Tolerant

Climate change is emerging phenomena and causing frequent drought which lead to scaricity of water, which ultimately nagetively affecting wheat (Triticumaestivum L.) yield all around the world. The aim of this study was to explore the potential deought tolerant wheat genotypes for candidate genes exploration. This study was conducted during the year 2014-2015 at Plant Physiology Division, Nuclear Institute of Agriculture (NIA) Tandojam. The six wheat genotypes (cv. MT-1/13, MT-2/13, MT-3/13, MT-4/13 Chakwal-86 and Khirman) were investigated for their response at germination and seedling stage under different water stress treatments (0, -0.5, -0.75 and -1.0 MPa) in controlled conditions. The results of experiments with reference to genotypes revealed that genotype Chakwal-86 shows maximum seed germination (82.58 %), while the genotype Khirman shows maximum shoot length  (7.23 cm), root length  (15.1 cm), shoot fresh wt. (5.85 g 10-1shoots), root fresh wt.  (3.45 g 10-1roots), shoot dry wt. (1.33 g 10-1shoots), root dry wt. (0.69 g 10-1roots). Among the genotypes tested Khirman and MT-4/13 are the tolerant genotypes had the potential to perform better under drought conditions, whereas  MT-4/13 and Chakwal-86 were moderate tolerant under water stress conditions. Moreover, the genotypes i.e. MT-1/13 and MT-2/13 are the sensitive genotypes under drought environment. It is concluded from present in-vitro studies that osmotic stress significantly reduced the seed germination shoot/root length fresh and dry weight in all six wheat genotypes. The maximum reduction was found at higher osmotic stress induced by PEG-6000 (-1.0 MPa) significantly.

scholarly journals INFLUENCE OF CADMIUM ON GROWTH AND BIOCHEMICAL CONTENTS OF TOMATO PLANTS

1970 ◽  
pp. 04-06
Author(s):  
A. Natarajan, P. Vijayarengan ◽  
M. Vijayaragavan
Keyword(s):  
Root Length ◽  
Growth Parameters ◽  
Sandy Loam ◽  
Shoot Length ◽  
Phenol Content ◽  
Tomato Plants ◽  
Biochemical Constituents ◽  
Root And Shoot Length ◽  
Soil Cadmium ◽  
Loam Soil

The increasing concentrations (10, 25, 50, 75 and 100 mg /kg) of soil cadmium on growth and biochemical contents in tomato plants were analysed on 30th sampling days. Control plants were maintained separately. Plants were grown in pots containing 3 kg of air dried sandy loam soil and treated with different concentrations (mg/kg) of cadmium (0, 10, 25, 50, 75 and 100 ). Treatments decreased the growth parameters such as root and shoot length  and biochemical constituents such as, protein,(except, proline and  phenol content) contents in tomato plants compared to untreated plants. The shoot length of cadmium treated tomato plants was higher than the root length. proline and phenol content of root of  tomato plants was higher than the shoot.

Evaluation of Herbicidal Potential of Eucalyptus spp for Biological Control of Dactyloctenium aegyptium L.

2021 ◽  
Vol 20 (1) ◽  
pp. 186-191
Author(s):  
Amal Ehtaiwesh
Keyword(s):  
Foliar Application ◽  
Growth Traits ◽  
Negative Impact ◽  
Inhibitory Effect ◽  
Germination Percentage ◽  
Shoot Length ◽  
Dactyloctenium Aegyptium ◽  
Vigor Index ◽  
Root And Shoot Length ◽  
Germination Traits

Pots culture experiment was conducted to evaluates the herbicidal potential of Eucalyptus spp. (aceae) on growth of Dactyloctenium aegyptium L. (Poaceae). Leaves extract with concentrations of 10, 20 and 40% of Eucalyptus plant were applied as soil and foliar application to seeds and seedlings of D. aegyptium species.  The result indicated that the application of Eucalyptus leaves extracts caused significant reduction in germination traits, root and shoot length, seedling fresh and dry weights. Also, the result revealed that the Inhibitory effect of Eucalyptus extract on D. aegyptium L was more noticeable in plant growth traits than on germination traits. Results show that among the different concentration of Eucalyptus leave extracts used in this study, 40% was the most toxic and caused the significant effect on germination and growth traits of Dactyloctenium aegyptium. Application of 40% of Eucalyptus leave extract decreased germination percentage by 30%, mean daily germination by 45%, shoot length by 38%. root length by 54%, number of branches by 61%, number of leavesby59%, seedling fresh weights by 23%, seedling dry weights by 62% and seedling vigor index by 60%. The results of this study concluded that the use of Eucalyptus plants have the potential to be developed further as a bio-herbicide system to control weed such as Dactyloctenium aegyptium L. However, more researches are needed to evaluate the negative impact of Eucalyptus on crop growth.

scholarly journals In vitro ROOTING OF TENERA HYBRID OIL PALM (Elaeis guineensis Jacq.) PLANTS1

2018 ◽  
Vol 41 (4) ◽  
Author(s):  
Marlúcia Souza Pádua ◽  
Raíssa Silveira Santos ◽  
Luciano Vilela Paiva ◽  
Vanessa Cristina Stein ◽  
Luciano Coutinho Silva
Keyword(s):  
Somatic Embryogenesis ◽  
Growth Regulators ◽  
Oil Palm ◽  
Somatic Embryos ◽  
Large Scale ◽  
Elaeis Guineensis ◽  
Germination Percentage ◽  
Shoot Length ◽  
Indole Butyric Acid

ABSTRACT Oil palm is a woody monocot of economic importance due to high oil production from its fruits. Currently, the conventional method most used to propagate oil palm is seed germination, but success is limited by long time requirements and low germination percentage. An alternative for large-scale propagation of oil palm is the biotechnological technique of somatic embryogenesis. The rooting of plants germinated from somatic embryos is a difficult step, yet it is of great importance for later acclimatization and success in propagation. The aim of this study was to evaluate the effect of the auxins indole acetic acid (IAA) and indole butyric acid (IBA) on the rooting of somatic embryos of Tenera hybrid oil palm. Plants obtained by somatic embryogenesis were inoculated in modified MS medium with 10% sucrose and 0.6% agar and supplemented with IAA or IBA at concentrations of 5 µM, 10 µM, and 15 µM, and the absence of growth regulators. After 120 days, the presence of roots, root type, length of the longest root, number of roots, number of leaves, and shoot length were analyzed. Growth regulators were favorable to rooting; plants cultivated with IBA growth regulator at 15 µM showed higher rooting percentage (87%) and better results for the parameters of number of roots (1.33) and shoot length (9.83).

scholarly journals In vitro screening of rice genotypes using polyethylene glycol under drought stress

2016 ◽  
Vol 27 (2) ◽  
pp. 128-135 ◽  
Author(s):  
J Akte ◽  
S Yasmin ◽  
MJH Bhuiyan ◽  
F Khatun ◽  
J Roy ◽  
...  
Keyword(s):  
Water Stress ◽  
Drought Stress ◽  
Water Content ◽  
Relative Water Content ◽  
Dry Weight ◽  
Proline Accumulation ◽  
Shoot Length ◽  
Fresh Weight ◽  
Relative Water

Five rice varieties viz. Binadhan-4, Binadhan-5, Binadhan-6, Binadhan-10 and Iratom-24 were evaluated in vitro under different water stress conditions. Several parameters such as germination percentage, shoot length, root length, shoot-root ratio, fresh weight, dry weight, turgid weight, relative water content and proline accumulation were studied. Drought condition was created by MS medium supplemented with five treatments of PEG, with a control such as 0%, 1%, 2%, 3% and 4% of PEG. The highest germination (100%) was found in the variety Binadhan-10 under low water stress conditions induced by 1% PEG. Similarly, the highest percentage of germination was found in all varieties under control condition (0% PEG). The lowest percentage of germination was obtained in the variety Iratom-24. But under severe stress (4% PEG), the highest percentage of germination was found only in the variety Binadhan-10. Moreover, the variety Binadhan-10 was found to be the best at 4% PEG for shoot length, root length, shoot-root ratio, relative water content and also the best at 1% PEG for fresh weight, dry weight, turgid weight. Water stress decreased relative water content and increased proline accumulation in rice. The highest relative water content was recorded in the variety Binadhan-10 and the lowest value recorded in the variety Binadhan-5. The highest proline content was obtained from the binadhan-6 at the highest treatment (4% PEG). Binadhan-10 showed the best performance almost in all the parameters under drought stress because of its own nature of tolerancy.Progressive Agriculture 27 (2): 128-135, 2016

scholarly journals In vitro allelopathic effect of aqueous extracts of sugarcane on germination parameters of wheat

2017 ◽  
Vol 109 (2) ◽  
pp. 349 ◽  
Author(s):  
Abdul Majeed ◽  
Zahir MUHAMMAD ◽  
Manzoor HUSSAIN ◽  
Habib AHMAD
Keyword(s):  
Germination Percentage ◽  
Allelopathic Effect ◽  
Aqueous Extracts ◽  
Wheat Growth ◽  
Plant Parts ◽  
Seedling Biomass ◽  
Mean Germination Time ◽  
Germination Indices ◽  
Germination Parameters

Allelopathy – interactions among plants for resources along with competition – is a composite phenomenon which has spacious potentials of application in agriculture. Understanding of interactions among plants, particularly cultivated crops, may be helpful in modifying crop cultivation pattern with consequent yields increments. In this study, we investigated the allelopathic effects of aqueous extracts of root, stem peels and leaves of sugarcane (<em>Saccharum officinale</em> L.) cultivar <em>51</em> at concentrations 0, 2.5, 5.0, 7.5 and 10.0 g/l on germination indices and seedling biomass of wheat (<em>Triticum aestivum </em>L.) cultivar <em>Pirsabak-2005</em>. Results demonstrated that higher concentration (10.0 g/l) of extracts of root, stem peels and leaves significantly decreased mean germination time (MGT) but increased shoot and seminal root growth and seedling dry biomass; however, germination percentage was affected neither by extract concentration nor by plant parts used in the study. Extract concentrations up to 7.5 g/l had no effect on the studied parameters of wheat. Our result suggests that sugarcane’s allelopathy demonstrates healthy effects on wheat growth and that wheat could be cultivated in sequential rotation in field conditions.

scholarly journals Impact of halopriming on four wheat (Triticum aestivum L.) cultivars of balochistan under saline conditions

2019 ◽  
Vol 48 (4) ◽  
pp. 1091-1097
Author(s):  
Summi Buzdar ◽  
Ayesha Mushtaq ◽  
Sabeena Rizwan ◽  
Uzma Jabeen ◽  
Farrukh Bashir ◽  
...  
Keyword(s):  
Sodium Silicate ◽  
Priming Effect ◽  
Growth Parameters ◽  
Triticum Aestivum L ◽  
Shoot Length ◽  
Culture Solution ◽  
Wheat Cultivars ◽  
Root And Shoot Length ◽  
Mean Germination Time ◽  
Silicon Sources

Priming effect of silicon sources (silica gel and sodium silicate) on the seeds of four wheat cultivars of Balochistan was studied to determine their effectiveness in increasing relative salt tolerance. The study depicts that all priming treatments of silicon sources (except sodium silicate 2%) enhanced germination and reduced mean germination time (MGT) of seeds in comparison to the control i.e. hydropriming. After germination, the young seedlings were grown in hydroponics in Hoagland’s culture solution under controlled conditions in non-saline and saline (100 mM NaCl) environments. All the growth parameters (root and shoot length, fresh and dry weights of plants and chlorophyll content of leaves) severely reduced in hydroprimed seeds under saline environments, although increased by priming seeds with silicon sources. Thus silica compounds have potential effects to break the seed dormancy and improve the growth of wheat under salinity stress.

Brassinolide on In Vitro Growth of Apical Meristem of Bananas

2014 ◽  
Vol 8 (1) ◽  
Author(s):  
FLORENDA C. BALLESTEROS-TEMANEL
Keyword(s):  
Plant Growth ◽  
In Vitro Propagation ◽  
Apical Meristem ◽  
Basal Medium ◽  
Tissue Growth ◽  
Shoot Length ◽  
New Class ◽  
Randomized Design ◽  
Plant Growth Substances

Brassinosteroids (BRs) are new class of hormones noted to perform multiplephysiological functions in plant growth and development and have the potentialof influencing cell and tissue growth in vitro. Many naturally occurring BRs,including brassinolide, have been discovered, their mode of action and their growthpromoting activities on plants. The use of brassinolide in in vitro propagation isnew. The Murashige and Skoog (1962) medium was used as basal medium. Plantgrowth regulators - IAA, BA and BR - were added to the medium. The study usedthe Completely Randomized Design (CRD) in factorial with three replications.The cultivar of banana and plant growth substances affected the number of budsproduced, shoot length, root length, and stem girth. The interaction of thesetwo factors (cultivar x PGR) influenced the number of buds produced in vitroand the shoot length of the meriplants. The study shows that brassinolide has aninfluence on shoot induction, proliferation, and elongation of bananas in in vitro propagation.Keywords: Agriculture, in vitro propagation, induction, proliferation, elongation, apical meristem, plant growth regulators, cultivars, Isabela, Philippines

scholarly journals Impact of dye effluent on growth and chlorophyll content of Alfalfa (Medicago sativa l.)

2016 ◽  
Vol 5 (10) ◽  
pp. 1432
Author(s):  
Pratibha Mahawar* ◽  
Azra Akhtar
Keyword(s):  
Medicago Sativa ◽  
Seedling Growth ◽  
Root Length ◽  
Growth Parameters ◽  
Research Work ◽  
Life Time ◽  
Shoot Length ◽  
Chlorophyll Contents ◽  
Root And Shoot Length ◽  
The Impact

The textile industry plays an important role in the world economy as well as in our daily life time, it consumes large quantity of water and generates huge amount of waste water. The chemical reagents used in the textile sector are diverse in chemical composition ranging from inorganic to organic molecules. The presence of these chemicals will show detrimental effects on the germination process and growth of seedlings. Present research work has been carried out to study the impact of effluent at different concentrations (20%, 40%, 60%, 80%, 100%) on seed germination and seedling growth of Medicago sativa. On the 14th day of seedling growth, maximum root and shoot length were observed at 20% concentration of effluent i.e. 5.4cm (root length) and 5.1cm (shoot length) which increased 3.8% and 4.0% respectively in comparison to control. At high concentrations of treatment levels root length was decreased 2.04%, 19.2%, 26.5%, 51.0% respectively and shoot length was decreased 3.84%, 17.3%, 26.9%, 44.2% respectively at 40%, 60%, 80% and 100% treatment levels in comparison to control (4.9cm and 5.2cm respectively). Same trend was observed during estimation of dry weight and chlorophyll contents.  Inhibition of seedling germination and seedling growth at higher concentrations of effluent may be due to high level of dissolved solids which inhance the salinity. The present study concluded that the dyeing effluent waste significantly influence growth parameters of Medicago sativa.

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