Projectome: Set up and testing of a High Performance Computational Infrastructure for processing and visualizing neuro-anatomical information obtained using confocal ultra-microscopy techniques

AbstractAsbestos bodies are the histological hallmarks of asbestos exposure. Both conventional and advanced techniques are used to evaluate abundance and composition in histological samples. We previously reported the possibility of using synchrotron X-ray fluorescence microscopy (XFM) for analyzing the chemical composition of asbestos bodies directly in lung tissue samples. Here we applied a high-performance synchrotron X-ray fluorescence (XRF) set-up that could allow new protocols for fast monitoring of the occurrence of asbestos bodies in large histological sections, improving investigation of the related chemical changes. A combination of synchrotron X-ray transmission and fluorescence microscopy techniques at different energies at three distinct synchrotrons was used to characterize asbestos in paraffinated lung tissues. The fast chemical imaging of the XFM beamline (Australian Synchrotron) demonstrates that asbestos bodies can be rapidly and efficiently identified as co-localization of high calcium and iron, the most abundant elements of these formations inside tissues (Fe up to 10% w/w; Ca up to 1%). By following iron presence, we were also able to hint at small asbestos fibers in pleural spaces. XRF at lower energy and at higher spatial resolution was afterwards performed to better define small fibers. These analyses may predispose for future protocols to be set with laboratory instruments.

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Histochemical and Phytochemical Analysis of Lamium album subsp. album L. Corolla: Essential Oil, Triterpenes, and Iridoids

Molecules ◽

10.3390/molecules26144166 ◽

2021 ◽

Vol 26 (14) ◽

pp. 4166

Author(s):

Agata Konarska ◽

Elżbieta Weryszko-Chmielewska ◽

Anna Matysik-Woźniak ◽

Aneta Sulborska ◽

Beata Polak ◽

...

Keyword(s):

Essential Oil ◽

High Performance ◽

Glandular Trichomes ◽

Gas Chromatography Mass Spectrometry ◽

Phytochemical Analysis ◽

Lipophilic Compounds ◽

Histochemical Tests ◽

Microscopy Techniques ◽

Trichome Types ◽

First Time

The aim of this study was to conduct a histochemical analysis to localize lipids, terpenes, essential oil, and iridoids in the trichomes of the L. album subsp. album corolla. Morphometric examinations of individual trichome types were performed. Light and scanning electron microscopy techniques were used to show the micromorphology and localization of lipophilic compounds and iridoids in secretory trichomes with the use of histochemical tests. Additionally, the content of essential oil and its components were determined using gas chromatography-mass spectrometry (GC-MS). Qualitative analyses of triterpenes carried out using high-performance thin-layer chromatography (HPTLC) coupled with densitometric detection, and the iridoid content expressed as aucubin was examined with spectrophotometric techniques. We showed the presence of iridoids and different lipophilic compounds in papillae and glandular and non-glandular trichomes. On average, the flowers of L. album subsp. album yielded 0.04 mL/kg of essential oil, which was dominated by aldehydes, sesquiterpenes, and alkanes. The extract of the L. album subsp. album corolla contained 1.5 × 10−3 ± 4.3 × 10−4 mg/mL of iridoid aucubin and three triterpenes: oleanolic acid, β-amyrin, and β-amyrin acetate. Aucubin and β-amyrin acetate were detected for the first time. We suggest the use of L. album subsp. album flowers as supplements in human nutrition.

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Optimization of Dynamic Task Location within a Manipulator’s Workspace for the Utilization of the Minimum Required Joint Torques

Electronics ◽

10.3390/electronics10030288 ◽

2021 ◽

Vol 10 (3) ◽

pp. 288

Author(s):

Adam Wolniakowski ◽

Charalampos Valsamos ◽

Kanstantsin Miatliuk ◽

Vassilis Moulianitis ◽

Nikos Aspragathos

Keyword(s):

High Performance ◽

Human Robot Interaction ◽

Optimal Position ◽

End Effector ◽

Joint Torques ◽

Dynamic Task ◽

Arbitrary Position ◽

Simulation Results ◽

Set Up ◽

Task Placement

The determination of the optimal position of a robotic task within a manipulator’s workspace is crucial for the manipulator to achieve high performance regarding selected aspects of its operation. In this paper, a method for determining the optimal task placement for a serial manipulator is presented, so that the required joint torques are minimized. The task considered comprises the exercise of a given force in a given direction along a 3D path followed by the end effector. Given that many such tasks are usually conducted by human workers and as such the utilized trajectories are quite complex to model, a Human Robot Interaction (HRI) approach was chosen to define the task, where the robot is taught the task trajectory by a human operator. Furthermore, the presented method considers the singular free paths of the manipulator’s end-effector motion in the configuration space. Simulation results are utilized to set up a physical execution of the task in the optimal derived position within a UR-3 manipulator’s workspace. For reference the task is also placed at an arbitrary “bad” location in order to validate the simulation results. Experimental results verify that the positioning of the task at the optimal location derived by the presented method allows for the task execution with minimum joint torques as opposed to the arbitrary position.

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Thermal properties of polybenzoxazine exhibiting improved toughness: Blending with cyclodextrin and its derivatives

High Performance Polymers ◽

10.1177/09540083211013091 ◽

2021 ◽

pp. 095400832110130

Author(s):

Hailong Li ◽

Sipei Zhao ◽

Li Pei ◽

Zihe Qiao ◽

Ding Han ◽

...

Keyword(s):

Thermal Properties ◽

Hydrogen Bonds ◽

High Performance ◽

Practical Application ◽

Scanning Calorimetry ◽

Thermoset Resins ◽

Chemical Structures ◽

Microscopy Techniques ◽

Fractured Surface ◽

Scanning Electron

Polybenzoxazines are emerging as a class of high-performance thermoset polymers that can find their applications in various fields. However, its practical application is limited by its low toughness. The cyclic β-cyclodextrin and a newly synthesized derivative (β-cyclodextrin-MAH) were separately blended with benzoxazine to improve the toughness of polybenzoxazine. The results revealed that the maximum impact strength of the blend was 12.24 kJ·m−2 and 14.29 kJ·m−2 when 1 wt.% of β-Cyclodextrin and β-Cyclodextrin-MAH, respectively, were used. The strengths were 53% and 86% higher than that of pure polybenzoxazine. The curing reaction, possible chemical structures, and fractured surface were examined using differential scanning calorimetry, Fourier transform infrared spectroscopy, and scanning electron microscopy techniques to understand the mechanism of generation of toughness. The results revealed that the sea-island structure and the presence of hydrogen bonds between polybenzoxazine and β-cyclodextrin and β-cyclodextrin-MAH resulted in the generation of toughness. Furthermore, the curves generated during thermogravimetric analysis did not significantly change, revealing the good thermal properties of the system. The phase-separated structure and the hydrogen bonds present in the system can be exploited to prepare synergistically tough polybenzoxazine exhibiting excellent thermal properties. This can be a potential way of modifying the thermoset resins.

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Differential-Geometric Methods in Multibody Dynamics and Control

Volume 6: 5th International Conference on Multibody Systems, Nonlinear Dynamics, and Control, Parts A, B, and C ◽

10.1115/detc2005-84860 ◽

2005 ◽

Author(s):

P. Maißer

Keyword(s):

Configuration Space ◽

High Performance ◽

Multibody System ◽

Equations Of Motion ◽

Geometric Approach ◽

Motion Equations ◽

Constrained Mechanical Systems ◽

Dynamics And Control ◽

Set Up ◽

Lagrangian Motion

This paper presents a differential-geometric approach to the multibody system dynamics regarded as a point dynamics in a n-dimensional configuration space Rn. This configuration space becomes a Riemannian space Vn the metric of which is defined by the kinetic energy of the multibody system (MBS). Hence, all concepts and statements of the Riemannian geometry can be used to study the dynamics of MBS. One of the key points is to set up the non-linear Lagrangian motion equations of tree-like MBS as well as of constrained mechanical systems, the perturbed equations of motion, and the motion equations of hybrid MBS in a derivative-free manner. Based on this approach transformation properties can be investigated for application in real-time simulation, control theory, Hamilton mechanics, the construction of first integrals, stability etc. Finally, a general Lyapunov-stable force control law for underactuated systems is given that demonstrates the power of the approach in high-performance sports applications.

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LPC Blade and Non-Axisymmetric Hub Profiling Optimization Using Multi-Fidelity Non-Intrusive POD Surrogates

Volume 2C: Turbomachinery ◽

10.1115/gt2017-65106 ◽

2017 ◽

Cited By ~ 2

Author(s):

Tariq Benamara ◽

Piotr Breitkopf ◽

Ingrid Lepot ◽

Caroline Sainvitu

Keyword(s):

High Performance ◽

High Efficiency ◽

High Fidelity ◽

Present Contribution ◽

Compressor Blades ◽

High Fidelity Simulations ◽

Optimization Methodology ◽

Set Up ◽

Automated Optimization ◽

Proper Orthogonal

The present contribution proposes a Reduced Order Model based multi-fidelity optimization methodology for the design of highly loaded blades in low pressure compressors. Environmental, as well as, economical limitations applied to engine manufacturers make the design of modern turbofans an extremely complex task. A smart compromise has to be found to guarantee both a high efficiency and a high average stage loading imposed for mass reduction constraints, while satisfying stability requirements. The design of compressor blades, usually involves at the same time a dedicated parametrization set-up in highdimensional space and high-fidelity simulations capturing, at least, efficiency and stability as most impacting phenomena. Despite recent advances in the high-performance computing area, introducing high-fidelity simulations into automated optimization, or even surrogate assisted optimization, loops still stands as a endeavor for engineers. In this framework, the proposed methodology is based on multi-fidelity surrogate models capable of representing the physics at hand in reduced spaces inferred from both precise, albeit costly, high-fidelity simulations and abundant, yet less accurate lower-fidelity data. Finally, we investigate the coupling of the proposed hierarchised multi-fidelity non-intrusive Proper Orthogonal Decomposition based surrogates with an evolutionary algorithm to reduce the number of high-fidelity simulation calls towards the targeted optimum.

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An Intelligent Spam Detection Model Based on Artificial Immune System

Information ◽

10.3390/info10060209 ◽

2019 ◽

Vol 10 (6) ◽

pp. 209 ◽

Cited By ~ 8

Author(s):

Abdul Jabbar Saleh ◽

Asif Karim ◽

Bharanidharan Shanmugam ◽

Sami Azam ◽

Krishnan Kannoorpatti ◽

...

Keyword(s):

Negative Selection ◽

High Performance ◽

Detection Rate ◽

Single Individual ◽

Spam Filtering ◽

True Negative ◽

Negative Selection Algorithm ◽

Detection Model ◽

Set Up ◽

Negative Detection

Spam emails, also known as non-self, are unsolicited commercial or malicious emails, sent to affect either a single individual or a corporation or a group of people. Besides advertising, these may contain links to phishing or malware hosting websites set up to steal confidential information. In this paper, a study of the effectiveness of using a Negative Selection Algorithm (NSA) for anomaly detection applied to spam filtering is presented. NSA has a high performance and a low false detection rate. The designed framework intelligently works through three detection phases to finally determine an email’s legitimacy based on the knowledge gathered in the training phase. The system operates by elimination through Negative Selection similar to the functionality of T-cells’ in biological systems. It has been observed that with the inclusion of more datasets, the performance continues to improve, resulting in a 6% increase of True Positive and True Negative detection rate while achieving an actual detection rate of spam and ham of 98.5%. The model has been further compared against similar studies, and the result shows that the proposed system results in an increase of 2 to 15% in the correct detection rate of spam and ham.

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High Performance Organic Field-Effect Transistors and Integrated Inverters

MRS Proceedings ◽

10.1557/proc-665-c7.5 ◽

2001 ◽

Vol 665 ◽

Cited By ~ 16

Author(s):

A. Ullmann ◽

J. Ficker ◽

W. Fix ◽

H. Rost ◽

W. Clemens ◽

...

Keyword(s):

Field Effect ◽

High Performance ◽

Field Effect Transistors ◽

Low Cost ◽

Drain Current ◽

Organic Field Effect Transistors ◽

Metal Contacts ◽

Gate Electrodes ◽

Polymer Semiconductors ◽

Set Up

ABSTRACTIntegrated plastic circuits (IPCs) will become an integral component of future low cost electronics. For low cost processes IPCs have to be made of all-polymer Transistors. We present our recent results on fabrication of Organic Field-Effect Transistors (OFETs) and integrated inverters. Top-gate transistors were fabricated using polymer semiconductors and insulators. The source-drain structures were defined by standard lithography of Au on a flexible plastic film, and on top of these electrodes, poly(3-alkylthiophene) (P3AT) as semiconductor, and poly(4-hydroxystyrene) (PHS) as insulator were homogeneously deposited by spin-coating. The gate electrodes consist of metal contacts. With this simple set-up, the transistors exhibit excellent electric performance with a high source-drain current at source - drain and gate voltages below 30V. The characteristics show very good saturation behaviour for low biases and are comparable to results published for precursor pentacene. With this setup we obtain a mobility of 0.2cm2/Vs for P3AT. Furthermore, we discuss organic integrated inverters exhibiting logic capability. All devices show shelf-lives of several months without encapsulation.

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Quantification of Unknown Nanoscale Biomolecules Using the Average-Weight-Difference Method

Applied Sciences ◽

10.3390/app9010130 ◽

2019 ◽

Vol 9 (1) ◽

pp. 130 ◽

Cited By ~ 1

Author(s):

Kyungwoo Lee ◽

Yonghyun Choi ◽

Kyobum Kim ◽

Hyung-Jun Koo ◽

Jonghoon Choi

Keyword(s):

Difference Method ◽

High Performance ◽

Drug Delivery Systems ◽

Reliable Method ◽

Analytical Techniques ◽

Drug Analysis ◽

Average Weight ◽

Weight Difference ◽

Drug Molecules ◽

Set Up

In order to quantify the amount of drug molecules in drug delivery systems, analytical techniques such as high-performance liquid chromatography are used due to their accuracy and reliability. However, the instruments required for such techniques are expensive and not available in all laboratories. Therefore, in this study, we introduce a method that can be a relatively inexpensive and easy to perform drug analysis in almost any laboratory set-up. We have devised the “average-weight-difference method” within the limits of existing spectral analyses. By employing this method, we quantitatively analyzed the amount of isoniazid or doxorubicin molecules loaded onto β-glucan nanoparticles. This proved to be a relatively simple and reliable method and can be used to estimate the amount of nanoscale biomolecules before their analysis through expensive equipment in an environment where the instruments are not readily available.

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The Studying Fabrication and Application of the Multifunctional Frozen Triaxial Test Apparatus

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.353-356.388 ◽

2013 ◽

Vol 353-356 ◽

pp. 388-391

Author(s):

Shi Yin Zhang

Keyword(s):

Triaxial Test ◽

High Performance ◽

Frozen Soil ◽

Load Test ◽

Test Apparatus ◽

Test Parameter ◽

New Type ◽

Triaxial Creep ◽

Set Up ◽

Triaxial Creep Test

The frozen soil triaxial test apparatus is the basic equipment to study the mechanics property of the frozen soil. According to the norm of frozen soil test and the construction character of the present mine, the high-performance frozen soil triaxial test apparatus is made which could be used for many type tests, such as the static triaxial shear test, the dynamic triaxial test, the triaxial creep test, the routine triaxial strain route test, the routine triaxial proportion coordination test, the routine triaxial recycle load test, the routine triaxial concretion test and so on. The apparatus can realize the high surround pressure. During the test progress, the test parameter can be set up and the test date can be collected by the computer. The frozen soil triaxial test apparatus is a new type triaxial test apparatus.

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