scholarly journals Adipose Stromal Cell-Secretome Counteracts Profibrotic Signals From IPF Lung Matrices

2021 ◽  
Vol 12 ◽  
Author(s):  
Gwenda F. Vasse ◽  
Lisette Van Os ◽  
Marina De Jager ◽  
Marnix R. Jonker ◽  
Theo Borghuis ◽  
...  
Keyword(s):  
Lung Tissue ◽  
Animal Studies ◽  
Stromal Cell ◽  
Structural Integrity ◽  
Specific Protein ◽  
Lung Fibroblasts ◽  
Control Treatment ◽  
Fibrotic Lung Disease ◽  
And Control ◽  
Total Tissue

Introduction: Idiopathic pulmonary fibrosis (IPF) is a fibrotic lung disease characterized by excess deposition and altered structure of extracellular matrix (ECM) in the lungs. The fibrotic ECM is paramount in directing resident cells toward a profibrotic phenotype. Collagens, an important part of the fibrotic ECM, have been shown to be structurally different in IPF. To further understand the disease to develop better treatments, the signals from the ECM that drive fibrosis need to be identified. Adipose tissue-derived stromal cell conditioned medium (ASC-CM) has demonstrated antifibrotic effects in animal studies but has not been tested in human samples yet. In this study, the collagen structural integrity in (fibrotic) lung tissue, its interactions with fibroblasts and effects of ASC-CM treatment hereon were studied.Methods: Native and decellularized lung tissue from patients with IPF and controls were stained for denatured collagen using a collagen hybridizing peptide. Primary lung fibroblasts were seeded into decellularized matrices from IPF and control subjects and cultured for 7 days in the presence or absence of ASC-CM. Reseeded matrices were fixed, stained and analyzed for total tissue deposition and specific protein expression.Results: In both native and decellularized lung tissue, more denatured collagen was observed in IPF tissue compared to control tissue. Upon recellularization with fibroblasts, the presence of denatured collagen was equalized in IPF and control matrices, whereas total ECM was higher in IPF matrices than in the control. Treatment with ASC-CM resulted in less ECM deposition, but did not alter the levels of denatured collagen.Discussion: Our data showed that ASC-CM can inhibit fibrotic ECM-induced profibrotic behavior of fibroblasts. This process was independent of collagen structural integrity. Our findings open up new avenues for ASC-CM to be explored as treatment for IPF.

Appropriate sera for calibration and control of specific protein assays

1993 ◽  
Vol 53 ◽  
pp. 13-15 ◽  
Author(s):  
O. Blaabjerg ◽  
M. Blom ◽  
H. Gry ◽  
P. Hyltoft Petersen ◽  
A. Uldall
Keyword(s):  
Specific Protein ◽  
And Control ◽  
Protein Assays

scholarly journals The effect of mineral fertilisers and farmyard manure on grain and straw yield, quality and economical parameters of winter wheat

2020 ◽  
Vol 66 (No. 6) ◽  
pp. 249-256
Author(s):  
Lukáš Hlisnikovský ◽  
Milan Vach ◽  
Zdeněk Abrhám ◽  
Ladislav Mensik ◽  
Eva Kunzová
Keyword(s):  
Winter Wheat ◽  
Farmyard Manure ◽  
Market Price ◽  
Economic Benefits ◽  
Quality Parameters ◽  
Control Treatment ◽  
Yield Quality ◽  
Technology And Economy ◽  
And Control ◽  
Technological Methods

In the years 2011–2014, winter wheat grain yield, qualitative and economic parameters were evaluated according to different fertiliser treatments: (1) control: unfertilised treatment; (2) farmyard manure (FYM) and (3) FYM + NPK (farmyard manure applied together with mineral NPK). The highest yields (8.10 t/ha) were recorded in the FYM + NPK treatment, while significantly lower yields (6.20 t/ha and 5.73 t/ha) were recorded in FYM and control treatments, respectively. Similarly, statistically significantly higher values of the quality parameters were found in the FYM + NPK treatment (13.55% of crude protein content and 43.56 mL of Zeleny’s sedimentation test), compared to control (10% and 22.44 mL, respectively). The modelling expert system (AGROTEKIS-Crop Technology and Economy) was used for the evaluation of economy. This software is based on technological methods of cultivation and norms of material input costs and costs of individual mechanised works. The economic benefits and profitability were evaluated for three different levels of grain market price. The highest gross profit per ha was recorded in the FYM + NPK treatment. According to the gross profit, the control treatment provided better results than the FYM treatment.

Effect of Ethephon and Packaging Practices On Ripening of Mango cv. Maldah

2021 ◽  
Vol 19 (1) ◽  
pp. 155-163
Author(s):  
S Sapkota ◽  
D Kc ◽  
H Giri ◽  
M Saud ◽  
M Basnet ◽  
...  
Keyword(s):  
Chemical Properties ◽  
Control Treatment ◽  
Quality Performance ◽  
Plastic Packaging ◽  
Randomized Design ◽  
Acid Ratio ◽  
Ethephon Treatment ◽  
Completely Randomized Design ◽  
And Control ◽  
Juice Content

The present research was conducted on two factor Completely Randomized Design (CRD) with eight treatments and three replications. A set of experiments were carried out to evaluate the effect of postharvest ethephon treatment and packaging on ripening of mango cv. Maldah. The treatments consisted of ripening agent i.e., ethephon and control treatment under different packaging condition i.e., fiber with hole, fiber without hole, plastic with hole and plastic without hole. The result revealed that different packaging condition and ripening agents influenced the ripening behavior of mango. The highest TSS (15.26), sugar-acid ratio (23.66) and juice content (126.05) were recorded with fiber (without hole) and the lowest TSS (12.60), sugar-acid ratio (9.01) and juice content (116.05) with plastic (without hole). The highest TA (1.44) was recorded with plastic (without hole) and the lowest (0.66) with fiber (without hole). Similarly, the highest BT (2.83) was recorded with fiber (with hole) and the lowest (1.66) with plastic (without hole). Firmness, sweetness, TSS and juice content were the highest with the interaction effect of fiber bag (without hole) and ethephon treatment. In conclusion, mango fruits with ethephon treatment packed in fiber bag (without hole) enhances quality and ripening of mango whereas under controlled condition and without hole plastic packaging mangoes had low quality performance in terms of physio-chemical properties. SAARC J. Agric., 19(1): 155-163 (2021)

scholarly journals Integrity Management of Marine Structures; With Emphasis on Design for Structural Robustness

2018 ◽  
Author(s):  
Torgeir Moan
Keyword(s):  
Structural Damage ◽  
Structural Integrity ◽  
Oil And Gas ◽  
Offshore Structures ◽  
Design Standards ◽  
Limit States ◽  
Fabrication Errors ◽  
Integrity Management ◽  
Operational Errors ◽  
And Control

Based on relevant accident experiences with oil and gas platforms, a brief overview of structural integrity management of offshore structures is given; including an account of adequate design criteria, inspection, repair and maintenance as well as quality assurance and control of the engineering processes. The focus is on developing research based design standards for Accidental Collapse Limit States to ensure robustness or damage tolerance in view damage caused by accidental loads due to operational errors and to some extent abnormal structural damage due to fabrication errors. Moreover, it is suggested to provide robustness in cases where the structural performance is sensitive to uncertain parameters. The use of risk assessment to aid decisions in lieu of uncertainties affecting the performance of novel and existing offshore structures, is briefly addressed.

VEGF synthesis is induced by prostacyclin and TGF-β in distal lung fibroblasts from COPD patients and control subjects: Implications for pulmonary vascular remodelling

Respirology ◽  
2017 ◽  
Vol 23 (1) ◽  
pp. 68-75 ◽  
Author(s):  
Gunilla Westergren-Thorsson ◽  
Mariam Bagher ◽  
Annika Andersson-Sjöland ◽  
Lena Thiman ◽  
Claes-Göran Löfdahl ◽  
...  
Keyword(s):  
Lung Fibroblasts ◽  
Vascular Remodelling ◽  
Control Subjects ◽  
Copd Patients ◽  
Distal Lung ◽  
And Control ◽  
Pulmonary Vascular Remodelling

scholarly journals Frequency in the supply of Lactobacillus influence its probiotic effect for yellow tail lambari

2018 ◽  
Vol 48 (10) ◽  
Author(s):  
Adolfo Jatobá ◽  
Klayton Natan Moraes ◽  
Emanuel Fernandes Rodrigues ◽  
Leandro Marcolino Vieira ◽  
Marina Oliveira Pereira
Keyword(s):  
Intestinal Tract ◽  
Leukocyte Count ◽  
Hematological Parameters ◽  
Control Treatment ◽  
Productive Performance ◽  
Probiotic Lactobacillus ◽  
Final Weight ◽  
And Control ◽  
Lactobacillus Spp ◽  
Probiotic Effect

ABSTRACT: The aim of this research was to evaluate the changes in intestinal tract microbiota, hematological parameters and zootechnical indexes of yellowtail lambaris (Astyanax bimaculatus) fed with different probiotic supply frequencies (Lactobacillus spp.). Lambaris (1,200) were distributed in twelve boxes, divided in three treatments, 100% supply; 25% and 0% (control) of feeds supplemented with probiotic. After 87 days, fish fed with supplemented diet, regardless of frequency (100% and 25%), showed an increase in the total lactic acid bacteria count of 7.99±0.15 log CFU mL-1 and 7.04±1, 29 log UFC mL-¹ respectively, in the intestinal tract and decrease of Pseudomonas spp. in both 100% and 25% (4.09±0.84log UFC mL-1 and 4.00±1.12log UFC mL-1, respectively) in relation to the control treatment (6.74±1.04log UFC mL-1). Fish fed with 100% of diets supplemented with probiotics had a higher leukocyte count (47.7±6.7x103μL-1), lymphocytes (36.0±2.6x103μL1), monocytes (12.0±4.2x103μl-1), as well as higher mean final weight (10.60±0.89g) and weekly growth rate (0.80±0.08g.week-1) than the other treatments (25% of probiotic offered and control). Probiotic (Lactobacillus spp.) offered in 25% of feeds (or more) alters the yellowtail lambari microbiota (A. bimaculatus); however, must be offer in 100% of feeds to improve the hematology profile and productive performance.

scholarly journals Foliar silicate application improves the tolerance of celery grown under heat stress conditions

2021 ◽  
Vol 117 (3) ◽  
pp. 1
Author(s):  
Fadl Abdelhamid HASHEM ◽  
Rasha M. EL-MORSHEDY ◽  
Tarek M. YOUNIS ◽  
Mohamed A. A. ABDRABBO
Keyword(s):  
Heat Stress ◽  
Vegetative Growth ◽  
Foliar Application ◽  
Climate Change Impacts ◽  
Potassium Silicate ◽  
Growth And Yield ◽  
Apium Graveolens ◽  
Control Treatment ◽  
F1 Hybrid ◽  
And Control

<p>Temperature rise is one of the most challenging climate change impacts that increase the intensity of heat stress. In this investigated the production of celery (<em>Apium graveolens</em> var. <em>rapaceum </em>F1 hybrid)) was tested during the late season. The experiment was carried out during the two successive summer seasons of 2019 and 2020 in Giza Governorate, Egypt. The experimental design is a split-plot, the main plots consist of three low tunnel cover treatments, and three spray treatments with three replicates in sub-main plots. Results showed that the use of white net cover gave the highest vegetative growth and yield followed by the black net. Values of plant yield were 951, 765, and 660 g/plant for white, black and without cover, respectively, in the first season. The foliar application of 3 mM of potassium silicate produced the highest vegetative growth and yield compared to the control treatment. Referring to the effect of spray foliar application of potassium silicate on yield 1.5 mM (S1), 3 mM (S2), and control were 892, 795, and 689 g/plant in the first season, respectively. The best combination that delivered the highest vegetative growth and yield was a cover low tunnel with a white net combined with S2 foliar application.</p>

scholarly journals Chronic Effects of Silver Nanoparticles on Micro-Crustacean Daphnia lumholtzi

2020 ◽  
Vol 36 (2) ◽  
Author(s):  
Tran Thanh Thai ◽  
Pham Thanh Luu ◽  
Ngo Xuan Quang ◽  
Dao Thanh Son
Keyword(s):  
Silver Nanoparticles ◽  
Chronic Toxicity ◽  
Exposure Period ◽  
Control Treatment ◽  
Reproduction Rate ◽  
Daphnia Lumholtzi ◽  
Multiple Generations ◽  
Toxicological Effects ◽  
Chronic Effects ◽  
And Control

This study aimed to enhance our insight on the potential toxicological effects of silver nanoparticles (AgNPs)  into the aquatic environment. To investigate the chronic toxicity of nanoparticles, freshwater micro-crustacean Daphnia lumholtzi was exposed to different concentrations of 0.2, 0.5 µg/l AgNPs, and control, for 21 days. Toxicological endpoints at different growing stages such as the maturation and reproduction were recorded. The reproduction rate of D. lumholtzi exposed to both AgNPs concentrations (0.2 and 0.5 µg/l ) was significantly lower than that of control. In turn, the maturation exposed to both AgNPs concentrations was not significantly different from the control treatment. This result indicates that AgNPs (with a concentration lower than 0.5 µg/l) did not have an adverse effect on the maturation of D. lumholtzi, but AgNPs with a concentration higher than 0.2 caused a toxic effect on the reproduction rate of D. lumholtzi during 21 days of the exposure period. In conclusion, the present results showed that AgNPs have toxic effects on D. lumholtzi and it has the potential to use as good freshwater aquatic zooplankton for assessment on the toxicity of nanomaterials in tropics. The future study should pay more attention to the effect of AgNPs on survival, growth rate, and multiple generations of daphnids to better understand the effects of nanoparticles in general and AgNPs in particular.

scholarly journals Phytoremediation as a Prospective Method for Rehabilitation of Areas Contaminated by Long-Term Sewage Sludge Storage: The Greek Experience

2017 ◽  
pp. 836-853
Author(s):  
N. Suchkova ◽  
E. Darakas ◽  
J. Ganoulis ◽  
Y. Vergeles
Keyword(s):  
Sewage Sludge ◽  
Plant Species ◽  
Shoot Growth ◽  
The Other ◽  
Control Treatment ◽  
Distilled Water ◽  
Series Of Experiments ◽  
And Control ◽  
Control Samples

In this work the suitability of several plant species for phytoremediation under natural and artificially installed conditions was studied. Brassica napus, Medicago sativa, Zea mays, Triticum aestivum, and Hordeum vulgare were grown in pots with sewage sludge from Sindos Main WWTP in Thessaloniki and from Sindos Industrial WWTP in Thessaloniki, Greece. The first series of experiments included comparing measurements of various parameters for the above mentioned plants and the sludge to those for control samples (the same plants growing in compost). It was shown that shoot growth was less reduced in T. aestivum and H. vulgare than in the other plant species studied. B. napus had lower germination tax, followed by M. sativa with the lowest germination tax. Generally B. napus, giving less biomass production than Z. mays and T. aestivum, is characterised by a higher ability to accumulate heavy metals like Cd, Cu, Ni, Pb, Zn, Cr, As and Hg. The second series of experiments included comparing measurements of various parameters of B. napus grown in sludge and treated each 7 days with metal solutions of Ni, Zn and Pb containing 10-2mg/l, 10-4mg/l, 10-6mg/l of each metal, to those for control plants treated with double distilled water. Results showed that shoot growth of B. napus were increased at treatments with lower concentrations of metals (10-6 mg/l) and control (treatment with d-distilled water). At the same time uptake of metal ions was increased with the concentration of the solution, i.e. at higher concentrations (10-2 mg/l). It is truly for Pb and Zn, transfer coefficient TC of which (indicates a plant’s potential to concentrate a metal) was quite high 15 % and 7 % correspondingly. It was noticed that B. napus has high ability to accumulate Cr, from the other hand it did not accumulate Ni (at present case).

scholarly journals KINETICS OF PHOSPHORUS RELEASE FROM ADDED ROCK PHOSPHATE WITH SOME ORGANIC FERTILIZERS ON CALCAREOUS SOIL.

2016 ◽  
Vol 47 (5) ◽  
Author(s):  
K.M. Naser
Keyword(s):  
Rock Phosphate ◽  
Kinetic Equations ◽  
Phosphorus Release ◽  
Organic Fertilizers ◽  
Chicken Manure ◽  
Control Treatment ◽  
Peat Moss ◽  
Silty Clay ◽  
Sheep Manure ◽  
And Control

Laboratory experiment was conducted using silty clay loam soil collected from Agriculture fields in Abu Ghraib to study the release of phosphorus from rock phosphate (10.22 P%) on  a soil treated with three types of organic fertilizers namely; peat moss , sheep manure  , poultry manure and  control treatment (soil only). Ten grams of air dried soil  mixed with 100 mg of ground rock phosphate  and  organic fertilizers added at 2.5% level (w:w) were  incubated at a laboratory temperature (298 K ) after adding  water  up  to two-thirds of the field capacity for 40, 90, 120, 150 and 180 days. Citric  acide  soluble  available phosphorous  was estimated after each incubation periods and kinetic equations used to test its release from treated samples. The additions of chicken manure was the highest in the  amount  of  dissolved phosphorus values followed by sheep manure, peat moss, rock phosphate and control treatment, reaching levels of citric  acide  extractable  phosphorus to  4.2 , 3.8, 3.3, 3.1 and 2.5 mg P kg-1 soil, respectively. The results also showed superiority of first  order equation in the description of phosphorus release from rock phosphate with  release  rate   coefficient  of 3.801 , 3.865 , 4.328 and 4.366 mg P kg -1 soil h-1 for the treatments: soil and rock phosphate only , peat moss, sheep waste and chicken manure, respectively.

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