scholarly journals Small RNAs: The Essential Regulators in Plant Thermotolerance

2021 ◽  
Vol 12 ◽  
Author(s):  
Zhi-Fang Zuo ◽  
Wenbo He ◽  
Jing Li ◽  
Beixin Mo ◽  
Lin Liu
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Stress Responses ◽  
Small Rnas ◽  
High Throughput Sequencing ◽  
High Temperature Stress ◽  
Temperature Acclimation ◽  
Regulatory Mechanisms ◽  
Small Interfering Rnas ◽  
Heat Stress Response

Small RNAs (sRNAs) are a class of non-coding RNAs that consist of 21–24 nucleotides. They have been extensively investigated as critical regulators in a variety of biological processes in plants. sRNAs include two major classes: microRNAs (miRNAs) and small interfering RNAs (siRNAs), which differ in their biogenesis and functional pathways. Due to global warming, high-temperature stress has become one of the primary causes for crop loss worldwide. Recent studies have shown that sRNAs are involved in heat stress responses in plants and play essential roles in high-temperature acclimation. Genome-wide studies for heat-responsive sRNAs have been conducted in many plant species using high-throughput sequencing. The roles for these sRNAs in heat stress response were also unraveled subsequently in model plants and crops. Exploring how sRNAs regulate gene expression and their regulatory mechanisms will broaden our understanding of sRNAs in thermal stress responses of plant. Here, we highlight the roles of currently known miRNAs and siRNAs in heat stress responses and acclimation of plants. We also discuss the regulatory mechanisms of sRNAs and their targets that are responsive to heat stress, which will provide powerful molecular biological resources for engineering crops with improved thermotolerance.

Identification and Characterization of Heat-Responsive miRNAs and their Regulatory Network in Maize

2020 ◽  
Author(s):  
Yang Zhao ◽  
Qiye Wei ◽  
Tianci Chen ◽  
Lijuan Xu ◽  
Jing Liu ◽  
...  
Keyword(s):  
Heat Stress ◽  
Regulatory Network ◽  
High Throughput Sequencing ◽  
Target Genes ◽  
Regulatory Mechanisms ◽  
Heat Stress Response ◽  
Yield And Quality ◽  
And Control ◽  
Identification And Characterization

Abstract Background: MicroRNAs (miRNAs) are a class of small non-coding RNAs, which have been demonstrated to play essential roles in plant growth and development, and in responses to abiotic stress. Heat stress is one of the most serious stresses that affecting crop yield and quality, however, the related regulatory mechanisms of miRNAs remains poorly understanding in maize. Results: In this study, a total of 340 miRNAs, including 215 known and 125 novel members, were identified from maize seedlings under heat stress (MH) and control conditions (MC) using high-throughput sequencing approach. The 215 known miRNAs can be further divided into 40 different families, and 21-nt miRNAs were found to be most abundant among the known miRNAs. Thirty-five miRNAs, including 26 known and 9 novel members, were significantly different expressed between MC and MH libraries. Furthermore, 174 target genes were predicted to be cleaved by 115 miRNAs using degradome sequencing. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses were performed for these targets to explore the biological function and pathways involved. Based on the relationships of miRNAs, target genes and the enriched results, a regulatory network was constructed for the miRNAs and their respective target genes, and 16 significantly differently expressed miRNAs (DEMs) were involved in the network. Conclusions: The results revealed novel insights into the roles of miRNAs in heat stress response and provided a useful foundation for understanding the regulatory mechanisms of heat-responsive miRNAs in maize.

scholarly journals Heat Stress Related Physiological and Metabolic Traits in Peanut Seedlings

2016 ◽  
Vol 43 (1) ◽  
pp. 24-35 ◽  
Author(s):  
D. Singh ◽  
M. Balota ◽  
E. Collakova ◽  
T.G. Isleib ◽  
G.E. Welbaum ◽  
...  
Keyword(s):  
Heat Stress ◽  
Fatty Acid ◽  
High Temperature ◽  
Unsaturated Fatty Acid ◽  
Stress Responses ◽  
High Temperature Stress ◽  
Growth Stages ◽  
Membrane Injury ◽  
Acid Ratio ◽  
High Yields

ABSTRACT To maintain high yields under an increasingly hotter climate, high temperature resilient peanut cultivars would have to be developed. Therefore, the mechanisms of plant response to heat need to be understood. The objective of this study was to explore the physiological and metabolic mechanisms developed by virginia-type peanut at early growth stages in response to high temperature stress. Peanut seedlings were exposed to 40/35 C (heat) and 30/25 C (optimum temperature) in a growth chamber. Membrane injury (MI), the Fv/Fm ratio, and several metabolites were evaluated in eight genotypes at four time-points (day 1, 2, 4, and 7) after the heat stress treatment initiation. Even though we were able to highlight some metabolites, e.g., hydroxyproline, galactinol, and unsaturated fatty acid, explaining specific differential physiological (MI) responses in peanut seedlings, overall our data suggested general stress responses rather than adaptive mechanisms to heat. Rather than individual metabolites, a combination of several metabolites better explained (41 to 61%) the MI variation in heat stressed peanut seedlings. The genotype SPT 06-07 exhibited lower MI, increased galactinol, reduced hydroxyproline, and higher saturated vs. unsaturated fatty acid ratio under heat stress compared to other genotypes. SPT 06-07 was also separated from the other genotypes during hierarchical clustering and, based on this and previous fieldwork, SPT 06-07 is proposed as a potential source for heat tolerance improvement of virginia-type peanut.

scholarly journals bZIP17 regulates heat stress tolerance at reproductive stage in Arabidopsis

aBIOTECH ◽  
2021 ◽  
Author(s):  
Juan Gao ◽  
Mei-Jing Wang ◽  
Jing-Jing Wang ◽  
Hai-Ping Lu ◽  
Jian-Xiang Liu
Keyword(s):  
Heat Stress ◽  
Stress Responses ◽  
High Throughput Sequencing ◽  
Reproductive Stage ◽  
Heat Stress Response ◽  
Unfolded Protein ◽  
Heat Stress Tolerance ◽  
The Unfolded Protein Response ◽  
Protein Response

AbstractHigh temperature elicits a well-conserved response called the unfolded protein response (UPR) to bring protein homeostasis in the endoplasmic reticulum (ER). Two key UPR regulators bZIP28 and bZIP60 have been shown to be essential for maintaining fertility under heat stress conditions in Arabidopsis, however, the function of transcriptional activator bZIP17, a paralog of bZIP28, in heat stress response at reproductive stage is not reported. Here we found that bzip17 mutant plants were sensitive to heat stress in terms of silique length and fertility comparing to that of wildtype (WT) Arabidopsis plants, and transcriptomic analysis showed that 1380 genes were specifically up-regulated and 493 genes were specifically down-regulated by heat stress in the flowers of WT plants comparing to that in bzip17 mutant plants. These bZIP17-dependent up-regulated genes were enriched in responses to abiotic stresses such as water deprivation and salt stress. Further chromatin immuno-precipitation coupled with high-throughput sequencing (ChIP-Seq) uncovered 1645 genes that were direct targets of bZIP17 in MYC-bZIP17 expressing seedlings subjected to heat stress. Among these 1645 genes, ERSE-II cis-element was enriched in the binding peaks of their promoters, and the up-regulation of 113 genes by heat stress in flowers was dependent on bZIP17. Our results revealed direct targets of bZIP17 in flowers during heat stress responses and demonstrated the important role of bZIP17 in maintaining fertility upon heat stress in plants.

scholarly journals Identification of Potential Genes Responsible for Thermotolerance in Wheat under High Temperature Stress

Genes ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 174 ◽  
Author(s):  
Peipei Su ◽  
Cai Jiang ◽  
Hao Qin ◽  
Rui Hu ◽  
Jialu Feng ◽  
...  
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Expression Profiles ◽  
Flag Leaf ◽  
Gene Expression Profiles ◽  
High Temperature Stress ◽  
Flavonoid Biosynthesis ◽  
Wheat Grain ◽  
Heat Stress Response ◽  
Differential Expressed Genes

Wheat, a major worldwide staple food crop, is relatively sensitive to a changing environment, including high temperature. The comprehensive mechanism of heat stress response at the molecular level and exploitation of candidate tolerant genes are far from enough. Using transcriptome data, we analyzed the gene expression profiles of wheat under heat stress. A total of 1705 and 17 commonly differential expressed genes (DEGs) were identified in wheat grain and flag leaf, respectively, through transcriptome analysis. Gene Ontology (GO) and pathway enrichment were also applied to illustrate the functions and metabolic pathways of DEGs involved in thermotolerance of wheat grain and flag leaf. Furthermore, our data suggest that there may be a more complex molecular mechanism or tighter regulatory network in flag leaf than in grain under heat stress over time, as less commonly DEGs, more discrete expression profiles of genes (principle component analysis) and less similar pathway response were observed in flag leaf. In addition, we found that transcriptional regulation of zeatin, brassinosteroid and flavonoid biosynthesis pathways may play an important role in wheat’s heat tolerance. The expression changes of some genes were validated using quantitative real-time polymerase chain reaction and three potential genes involved in the flavonoid biosynthesis process were identified.

scholarly journals Wheat (Triticum aestivum L.) TaHMW1D Transcript Variants Are Highly Expressed in Response to Heat Stress and in Grains Located in Distal Part of the Spike

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 687
Author(s):  
Chan Seop Ko ◽  
Jin-Baek Kim ◽  
Min Jeong Hong ◽  
Yong Weon Seo
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Temperature Stress ◽  
Storage Proteins ◽  
Seed Storage ◽  
Seed Storage Proteins ◽  
Grain Filling ◽  
High Temperature Stress ◽  
Two Dimensional Gel Electrophoresis ◽  
Transcript Variants

High-temperature stress during the grain filling stage has a deleterious effect on grain yield and end-use quality. Plants undergo various transcriptional events of protein complexity as defensive responses to various stressors. The “Keumgang” wheat cultivar was subjected to high-temperature stress for 6 and 10 days beginning 9 days after anthesis, then two-dimensional gel electrophoresis (2DE) and peptide analyses were performed. Spots showing decreased contents in stressed plants were shown to have strong similarities with a high-molecular glutenin gene, TraesCS1D02G317301 (TaHMW1D). QRT-PCR results confirmed that TaHMW1D was expressed in its full form and in the form of four different transcript variants. These events always occurred between repetitive regions at specific deletion sites (5′-CAA (Glutamine) GG/TG (Glycine) or (Valine)-3′, 5′-GGG (Glycine) CAA (Glutamine) -3′) in an exonic region. Heat stress led to a significant increase in the expression of the transcript variants. This was most evident in the distal parts of the spike. Considering the importance of high-molecular weight glutenin subunits of seed storage proteins, stressed plants might choose shorter polypeptides while retaining glutenin function, thus maintaining the expression of glutenin motifs and conserved sites.

scholarly journals Widespread occurrence of microRNA-mediated target cleavage on membrane-bound polysomes

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Xiaoyu Yang ◽  
Chenjiang You ◽  
Xufeng Wang ◽  
Lei Gao ◽  
Beixin Mo ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Gene Silencing ◽  
High Frequency ◽  
Small Rnas ◽  
High Throughput Sequencing ◽  
Noncoding Rnas ◽  
Small Interfering Rnas ◽  
Widespread Occurrence ◽  
Membrane Bound ◽  
Different Tissues

Abstract Background Small RNAs (sRNAs) including microRNAs (miRNAs) and small interfering RNAs (siRNAs) serve as core players in gene silencing at transcriptional and post-transcriptional levels in plants, but their subcellular localization has not yet been well studied, thus limiting our mechanistic understanding of sRNA action. Results We investigate the cytoplasmic partitioning of sRNAs and their targets globally in maize (Zea mays, inbred line “B73”) and rice (Oryza sativa, cv. “Nipponbare”) by high-throughput sequencing of polysome-associated sRNAs and 3′ cleavage fragments, and find that both miRNAs and a subset of 21-nucleotide (nt)/22-nt siRNAs are enriched on membrane-bound polysomes (MBPs) relative to total polysomes (TPs) across different tissues. Most of the siRNAs are generated from transposable elements (TEs), and retrotransposons positively contributed to MBP overaccumulation of 22-nt TE-derived siRNAs (TE-siRNAs) as opposed to DNA transposons. Widespread occurrence of miRNA-mediated target cleavage is observed on MBPs, and a large proportion of these cleavage events are MBP-unique. Reproductive 21PHAS (21-nt phasiRNA-generating) and 24PHAS (24-nt phasiRNA-generating) precursors, which were commonly considered as noncoding RNAs, are bound by polysomes, and high-frequency cleavage of 21PHAS precursors by miR2118 and 24PHAS precursors by miR2275 is further detected on MBPs. Reproductive 21-nt phasiRNAs are enriched on MBPs as opposed to TPs, whereas 24-nt phasiRNAs are nearly completely devoid of polysome occupancy. Conclusions MBP overaccumulation is a conserved pattern for cytoplasmic partitioning of sRNAs, and endoplasmic reticulum (ER)-bound ribosomes function as an independent regulatory layer for miRNA-induced gene silencing and reproductive phasiRNA biosynthesis in maize and rice.

Pre-anthesis high-temperature acclimation alleviates damage to the flag leaf caused by post-anthesis heat stress in wheat

2011 ◽  
Vol 168 (6) ◽  
pp. 585-593 ◽  
Author(s):  
Xiao Wang ◽  
Jian Cai ◽  
Dong Jiang ◽  
Fulai Liu ◽  
Tingbo Dai ◽  
...  
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Flag Leaf ◽  
Temperature Acclimation

Emergence of seedlings from different depths following high temperature stress

1975 ◽  
Vol 84 (3) ◽  
pp. 525-528 ◽  
Author(s):  
I. C. Onwueme ◽  
S. A. Adegoroye
Keyword(s):  
Heat Stress ◽  
High Temperature ◽  
Temperature Stress ◽  
Seedling Emergence ◽  
High Temperature Stress ◽  
Moist Soil ◽  
Different Depths

SUMMARYSeeds of Amaranthus, melon, cowpea and tomato were planted in moist soil at 1, 4 or 7·5 cm depth and subjected to a heat stress of 45 °C for 10 h on the day of sowing (day 0), 1 day after sowing or 2 days after sowing. Seedling emergence was retarded by heat stress, the most drastic retardation being due to heat stress on day 1 for cowpea and tomato, day 2 for melon, and day 0 for Amaranthus. Emergence also decreased with increasing depth of sowing. The interaction of depth and heat stress was also significant in all cases, such that the delay in emergence due to heat stress tended to be greater with increasing depth of sowing. The agronomic significance of the results is discussed.

scholarly journals An atypical class of non-coding small RNAs produced in rice leaves upon bacterial infection

2021 ◽  
Author(s):  
Ganna Reshetnyak ◽  
Jonathan M. Jacobs ◽  
Florence Auguy ◽  
Coline Sciallano ◽  
Lisa Claude ◽  
...  
Keyword(s):  
Gene Silencing ◽  
Stress Responses ◽  
Small Rnas ◽  
Early Stage ◽  
Kinase Domain ◽  
Transcriptional Gene Silencing ◽  
Small Interfering Rnas ◽  
Post Transcriptional Gene Silencing ◽  
Virulent Strains ◽  
Microbe Interactions

ABSTRACTNon-coding small RNAs (sRNA) act as mediators of gene silencing and regulate plant growth, development and stress responses. Early insights into plant sRNAs established a role in antiviral defense and they are now extensively studied across plant-microbe interactions. Here, sRNA sequencing discovered a class of sRNA in rice (Oryza sativa) specifically associated with foliar diseases caused by Xanthomonas oryzae bacteria. Xanthomonas-induced small RNAs (xisRNAs) loci were distinctively upregulated in response to diverse virulent strains at an early stage of infection producing a single duplex of 20-22nt sRNAs. xisRNAs production was dependent on the Type III secretion system, a major bacterial virulence factor for host colonization. xisRNA loci overlap with annotated transcripts sequences often encoding protein kinase domain proteins. A number of the corresponding rice cis-genes have documented functions in immune signaling and some xisRNA loci coincide with the coding sequence of a conserved kinase motif. xisRNAs exhibit features of small interfering RNAs and their biosynthesis depend on canonical components OsDCL1 and OsHEN1. xisRNA induction possibly mediates post-transcriptional gene silencing but they do not broadly suppress cis-genes expression on the basis of mRNA-seq data. Overall, our results identify a group of unusual sRNAs with a potential role in plant-microbe interactions.

scholarly journals Heat Stress Alters the Intestinal Microbiota and Metabolomic Profiles in Mice

2021 ◽  
Vol 12 ◽  
Author(s):  
Chaoyue Wen ◽  
Siyu Li ◽  
Jiaojiao Wang ◽  
Yimin Zhu ◽  
Xin Zong ◽  
...  
Keyword(s):  
Heat Stress ◽  
Fatty Acid ◽  
High Temperature ◽  
Intestinal Microbiota ◽  
High Throughput Sequencing ◽  
Acid Metabolism ◽  
Core Body Temperature ◽  
Control Group ◽  
Serum Biochemical ◽  
The Impact

BackgroundHeat stress has negative effects on the intestinal health of humans and animals. However, the impact of heat stress on intestinal microbial and metabolic changes remains elusive. Here, we investigated the cecal microbial and metabolic profiles in mice in response to heat stress.MethodsThe mouse heat stress model was constructed by simulating a high-temperature environment. Twenty mice were randomly assigned to two groups, the control group (CON, 25°C) and the heat treatment group (HS, 40°C from 13:00 to 15:00 every day for 7 days). Serum and cecal contents were collected from the mice for serum biochemical analysis, 16S rRNA high-throughput sequencing, and non-targeted metabolomics.ResultsBoth core body temperature and water intake were significantly increased in the HS group. Serum biochemical indicators were also affected, including significantly increased triglyceride and decreased low-density lipoprotein in the heat stress group. The composition and structure of intestinal microbiota were remarkably altered in the HS group. At the species level, the relative abundance of Candidatus Arthromitus sp. SFB-mouse-Japan and Lactobacillus murinus significantly reduced, while that of Lachnospiraceae bacterium 3-1 obviously increased after HS. Metabolomic analysis of the cecal contents clearly distinguished metabolite changes between the groups. The significantly different metabolites identified were mainly involved in the fatty acid synthesis, purine metabolism, fatty acid metabolism, cyanoamino acid metabolism, glyceride metabolism, and plasmalogen synthesis.ConclusionIn summary, high temperature disrupted the homeostatic balance of the intestinal microbiota in mice and also induced significant alterations in intestinal metabolites. This study provides a basis for treating intestinal disorders caused by elevated temperature in humans and animals and can further formulate nutritional countermeasures to reduce heat stress-induced damage.

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