Hormonome Dynamics During Microgametogenesis in Different Nicotiana Species

Plant microgametogenesis involves stages leading to the progressive development of unicellular microspores into mature pollen. Despite the active and continuing interest in the study of male reproductive development, little is still known about the hormonomics at each ontogenetic stage. In this work, we characterized the profiles and dynamics of phytohormones during the process of microgametogenesis in four Nicotiana species (Nicotiana tabacum, Nicotiana alata, Nicotiana langsdorffii, and Nicotiana mutabilis). Taking advantage of advanced HPLC-ESI-MS/MS, twenty to thirty endogenous hormone derivatives were identified throughout pollen ontogenesis, including cytokinins, auxins, ABA and its derivatives, jasmonates, and phenolic compounds. The spectra of endogenous phytohormones changed dynamically during tobacco pollen ontogeny, indicating their important role in pollen growth and development. The different dynamics in the accumulation of endogenous phytohormones during pollen ontogenesis between N. tabacum (section Nicotiana) and the other three species (section Alatae) reflects their different phylogenetic positions and origin within the genus Nicotiana. We demonstrated the involvement of certain phytohormone forms, such as cis-zeatin- and methylthiol-type CKs, some derivatives of abscisic acid, phenylacetic and benzoic acids, in pollen development for the first time here. Our results suggest that unequal levels of endogenous hormones and the presence of specific derivatives may be characteristic for pollen development in different phylogenetic plant groups. These results represent the currently most comprehensive study of plant hormones during the process of pollen development.

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Insight into the Secondary Metabolites of Geum urbanum L. and Geum rivale L. Seeds (Rosaceae)

Plants ◽

10.3390/plants10061219 ◽

2021 ◽

Vol 10 (6) ◽

pp. 1219

Author(s):

Marek Bunse ◽

Peter Lorenz ◽

Florian C. Stintzing ◽

Dietmar R. Kammerer

Keyword(s):

Fatty Acids ◽

Phenolic Compounds ◽

Unsaturated Fatty Acids ◽

Asiatic Acid ◽

Cosmetic Products ◽

Nutritional Properties ◽

First Time ◽

Derivatives Of ◽

Geum Urbanum ◽

Insight Into

The present study aimed at the identification and quantitation of phenolic compounds, fatty acids, and further characteristic substances in the seeds of Geum urbanum L. and Geum rivale L. For this purpose, individual components of extracts recovered with MeOH, CH2Cl2, and by cold-pressing, respectively, were characterized by HPLC-DAD/ESI-MSn and GC/MS and compared with reference compounds. For both Geum species, phenolic compounds, such as flavonoids and gallic acid derivatives, and triterpenes, such as saponins and their aglycones, were detected. Surprisingly, both Geum species revealed the presence of derivatives of the triterpenoid aglycons asiatic acid and madecassic acid, which were characterized for the first time in the genus Geum. Furthermore, the fatty acids of both species were characterized by GC–MS after derivatization. Both species showed a promising fatty-acid profile in terms of nutritional properties because of high proportions of unsaturated fatty acids. Linoleic acid and linolenic acid were most abundant, among other compounds such as palmitic acid and stearic acid. In summary, the present study demonstrates the seeds of G. urbanum and G. rivale to be a valuable source of unsaturated fatty acids and bioactive phenolics, which might be exploited for nutritional and cosmetic products and for phytotherapeutic purposes.

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Identification of pigments in artworks by inverse tangent derivative of spectrum and a new filtering method

Heritage Science ◽

10.1186/s40494-020-00438-4 ◽

2020 ◽

Vol 8 (1) ◽

Author(s):

F. Fazlali ◽

S. Gorji Kandi

Keyword(s):

Reflectance Spectrum ◽

Practical Method ◽

Chemical Methods ◽

Munk Function ◽

Absorption Of Light ◽

Spectrophotometric Data ◽

First Time ◽

Non Destructive ◽

Derivatives Of

Abstract Employing an economical and non-destructive method for identifying pigments utilized in artworks is a significant aspect for preserving their antiquity value. One of the non-destructive methods for this purpose is spectrophotometry, which is based on the selected absorption of light. Mathematical descriptive methods such as derivatives of the reflectance spectrum, the Kubelka–Munk function and logarithm have been employed for the characterization of the peak features corresponding to the spectrophotometric data. In the present study, the mentioned mathematical descriptive methods were investigated with the aim to characterize the constituents of an Iranian artwork but were not efficient for the samples. Therefore, inverse tangent derivative equation was developed on spectral data for the first time, providing considerable details in the profile of reflectance curves. In the next part, to have a simpler and more practical method it was suggested to use filters made up of pure pigments. By using these filters and placing them on the samples, imaging was done. Then, images of samples with and without filter were evaluated and pure pigments were distinguished. The mentioned methods were also used to identify pigments in a modern Iranian painting specimen. The results confirmed these methods with reliable answers indicating that physical methods (alongside chemical methods) can also be effective in determining the types of pigments.

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Synthesis of Galactomannan Sulfate-Citrate

Materials Science Forum ◽

10.4028/www.scientific.net/msf.1049.218 ◽

2022 ◽

Vol 1049 ◽

pp. 218-223

Author(s):

Aleksandr S. Kazachenko ◽

Yuriy N. Malyar ◽

Anna S. Kazachenko

Keyword(s):

Biological Activity ◽

Ftir Spectroscopy ◽

X Ray Diffraction ◽

Absorption Bands ◽

X Ray ◽

Mixed Ester ◽

First Time ◽

Derivatives Of

Sulfated derivatives of polysaccharides have anticoagulant, hypolipedimic and other biological activity. In this work, a complex mixed ester of galactomannan, its sulfate-citrate, was obtained for the first time. The introduction of citrate and sulfate groups was proved by FTIR spectroscopy by the appearance of corresponding absorption bands. It was shown by X-ray diffraction that the introduction of the citrate group leads to the amorphization of the galactomannan structure.

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Synthetic Derivatives of Natural Alkaloid Harmine

Eurasian Chemico-Technological Journal ◽

10.18321/ectj281 ◽

2016 ◽

Vol 11 (3) ◽

pp. 199

Author(s):

N.M. Ismagulova ◽

Zh.S. Nurmaganbetov ◽

A.Zh. Turmukhambetov ◽

T.S. Seitembetov ◽

S.M. Adekenov

Keyword(s):

Indole Alkaloid ◽

Quaternary Ammonium Salts ◽

Ammonium Salts ◽

Peganum Harmala ◽

Underground Part ◽

X Ray ◽

Natural Alkaloid ◽

First Time ◽

Synthetic Derivatives ◽

Derivatives Of

The indole alkaloid harmine was extracted from underground part of <em>Peganum harmala L.</em> With the purpose of obtaining the new biological active derivatives on base of alkaloid harmine the chemical modification was carried out. The p-toluolsulfochlorid, p-toluolsulfoacid, hydrochloric, sulfuric, nitric acids, dioxide selenium and phthalic anhydride have been chosen as modifiers. For the first time quaternary ammonium salts, derivatives of N-oxide and N (2)-oxyharminiumphthalate harmine are synthesized. The structure of the synthesized compounds is determined by methods of the spectral analysis and X-ray analysis. Antimicrobic and phagocytosis stimulating activities of isolated alkaloids and their derivatives are investigated.

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Trends and Exceptions in the Interaction of Hydroxamic Acid Derivatives of Common Di- and Tripeptides with Some 3d and 4d Metal Ions in Aqueous Solution

Molecules ◽

10.3390/molecules24213941 ◽

2019 ◽

Vol 24 (21) ◽

pp. 3941 ◽

Cited By ~ 2

Author(s):

Ozsváth ◽

Bíró ◽

Nagy ◽

Buglyó ◽

Sanna ◽

...

Keyword(s):

Metal Ions ◽

Hydroxamic Acid ◽

Hydroxamic Acids ◽

17O Nmr ◽

Equilibrium Study ◽

Sandwich Type ◽

Uv Visible ◽

First Time ◽

Derivatives Of ◽

Half Sandwich

By using various techniques (pH-potentiometry, UV-Visible spectrophotometry, 1H and 17O-NMR, EPR, ESI-MS), first time in the literature, solution equilibrium study has been performed on complexes of dipeptide and tripeptide hydroxamic acids—AlaAlaNHOH, AlaAlaN(Me)OH, AlaGlyGlyNHOH, and AlaGlyGlyN(Me)OH—with 4d metals: the essential Mo(VI) and two half-sandwich type cations, [(η6-p-cym)Ru(H2O)3]2+ as well as [(η5-Cp*)Rh(H2O)3]2+, the latter two having potential importance in cancer therapy. The tripeptide derivatives have also been studied with some biologically important 3d metals, such as Fe(III), Ni(II), Cu(II), and Zn(II), in order to compare these new results with the corresponding previously obtained ones on dipeptide hydroxamic acids. Based on the outcomes, the effects of the type of metal ions, the coordination number, the number and types of donor atoms, and their relative positions to each other on the complexation have been evaluated in the present work. We hope that these collected results might be used when a new peptide-based hydroxamic acid molecule is planned with some purpose, e.g. to develop a potential metalloenzyme inhibitor.

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Suppression of Methane Generation during Methanogenesis by Chemically Modified Humic Compounds

Antioxidants ◽

10.3390/antiox9111140 ◽

2020 ◽

Vol 9 (11) ◽

pp. 1140

Author(s):

Elena Efremenko ◽

Olga Senko ◽

Nikolay Stepanov ◽

Nikita Mareev ◽

Alexander Volikov ◽

...

Keyword(s):

Antioxidant Properties ◽

Nutrient Media ◽

Potassium Humate ◽

Methane Generation ◽

Chemically Modified ◽

Humic Compounds ◽

Negative Effect ◽

The Media ◽

First Time ◽

Derivatives Of

The introduction of various concentrations of chemically modified humic compounds (HC) with different redox characteristics into the media with free and immobilized anaerobic consortia accumulating landfill gases was studied as approach to their functioning management. For this purpose, quinone (hydroquinone, naphthoquinone or methylhydroquinone) derivatives of HC were synthesized, which made it possible to vary the redox and antioxidant properties of HC as terminal electron acceptors in methanogenic systems. The highest acceptor properties were obtained with potassium humate modified by naphthoquinone. To control possible negative effect of HC on the cells of natural methanogenic consortia, different bioluminescent analytical methods were used. The addition of HC derivatives, enriched with quinonones, to nutrient media at concentrations above 1 g/L decreased the energetic status of cells and the efficiency of the methanogenesis. For the first time, the significant decrease in accumulation of biogas was reached as effect of synthetic HC derivatives, whereas both notable change of biogas composition towards increase in the CO2 content and decrease in CH4 were revealed. Thus, modification with quinones makes it possible to obtain low-potential HC derivatives with strongly pronounced acceptor properties, promising for inhibition of biogas synthesis by methanogenic communities.

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Newly onset: sudden & severe headache in a patient who overused analgesic

Bangladesh Journal of Medical Science ◽

10.3329/bjms.v14i4.25806 ◽

2015 ◽

Vol 14 (4) ◽

pp. 399-401 ◽

Cited By ~ 1

Author(s):

Khairi Che Mat ◽

Rohayah Husain ◽

Nasir Mohamad

Keyword(s):

Medical Science ◽

Severe Headache ◽

Increased Intracranial Pressure ◽

Provisional Diagnosis ◽

Early Management ◽

Progressive Development ◽

Acute Headache ◽

First Time ◽

Space Occupying Lesion ◽

New Onset

This case report presented a rare cause of severe headache in a patient who developed episodes of acute severe headache for the first time. The earliest accurate provisional diagnosis is crucial for a new onset severe headache in adult. A good history taking and high index of suspicious are remained the most important clinical component in managing severe first time headache in adult. The differential diagnosis of subarachnoid hemorrhage, space-occupying lesion with increased intracranial pressure, acute intoxication, meningo-encephalitis and others must be ruled out as these conditions are benefited in early management. In this patient, progressive development of headache is masked by severe intense headache, which later will be described as acute headache. The causes of newly onset severe intense headache will be discussed in the discussion.Bangladesh Journal of Medical Science Vol.14(4) 2015 p.399-401

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A β‐galactosidase‐like gene is expressed during tobacco pollen development

Journal of Experimental Botany ◽

10.1093/jexbot/52.354.67 ◽

2001 ◽

Vol 52 (354) ◽

pp. 67-75 ◽

Cited By ~ 18

Author(s):

Hilary Joan Rogers ◽

Sarah Louise Maund ◽

Lisa Helen Johnson

Keyword(s):

Pollen Development ◽

Tobacco Pollen

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Processing and Stability of Inducibly Expressed rpsO mRNA Derivatives in Bacillus subtilis

Journal of Bacteriology ◽

10.1128/jb.00740-09 ◽

2009 ◽

Vol 191 (18) ◽

pp. 5680-5689 ◽

Cited By ~ 8

Author(s):

Shiyi Yao ◽

David H. Bechhofer

Keyword(s):

Bacillus Subtilis ◽

Early Step ◽

Stem Loop ◽

Processing Event ◽

Shine Dalgarno Sequence ◽

Ribosomal Protein S15 ◽

High Level ◽

First Time ◽

Derivatives Of

ABSTRACT The Bacillus subtilis rpsO gene specifies a small (388-nucleotide), monocistronic mRNA that encodes ribosomal protein S15. We showed earlier that rpsO mRNA decay intermediates accumulated to a high level in a strain lacking polynucleotide phosphorylase. Here, we used inducibly expressed derivatives of rpsO, encoding smaller RNAs that had the complex 5′ region deleted, to study aspects of mRNA processing in B. subtilis. An IPTG (isopropyl-β-d-thiogalactopyranoside)-inducible rpsO transcript that contained lac sequences at the 5′ end, called lac-rpsO RNA, was shown to undergo processing to result in an RNA that was 24 nucleotides shorter than full length. Such processing was dependent on the presence of an accessible 5′ terminus; a lac-rpsO RNA that contained a strong stem-loop at the 5′ end was not processed and was extremely stable. Interestingly, this stability depended also on ribosome binding to a nearby Shine-Dalgarno sequence but was independent of downstream translation. Either RNase J1 or RNase J2 was capable of processing lac-rpsO RNA, demonstrating for the first time a particular in vivo processing event that could be catalyzed by both enzymes. Decay intermediates were detected in the pnpA strain only for a lac-rpsO RNA that was untranslated. Analysis of processing of an untranslated lac-rpsO RNA in the pnpA strain shortly after induction of transcription suggested that endonuclease cleavage at 3′-proximal sites was an early step in turnover of mRNA.

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Comprehensive analysis of metabolites in Corynebacterium glutamicum by gas chromatography/mass spectrometry

Biological Chemistry ◽

10.1515/bc.2004.111 ◽

2004 ◽

Vol 385 (9) ◽

pp. 853-861 ◽

Cited By ~ 87

Author(s):

Sergey Strelkov ◽

Mirko von Elstermann ◽

Dietmar Schomburg

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Corynebacterium Glutamicum ◽

Growth Conditions ◽

Gas Chromatography Mass Spectrometry ◽

Fast Method ◽

Chromatography Mass Spectrometry ◽

High Matrix ◽

First Time ◽

Derivatives Of

AbstractAn analytical method based on gas chromatography/mass spectrometry was developed for metabolome investigation ofCorynebacterium glutamicum. For the first time a fast method for metabolic screening that can be automated is described for this organism. More than 1000 compounds could be detected per experiment, ca. 330 of those showed a peak area significantly above background. Out of these 164 compounds were identified so far, representing derivatives of 121 different metabolites, which were quantified in one sample. In spite of the different chemical nature of metabolites and high matrix content, a measurement reproducibility in the range of 6% error was achieved. The application of this method for the analysis of the adaptation ofC. glutamicumto different growth conditions is demonstrated.

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