scholarly journals Influence of Glycyrrhiza glabra Extract on Growth, Gene Expression of Gut Integrity, and Campylobacter jejuni Colonization in Broiler Chickens

2020 ◽  
Vol 7 ◽  
Author(s):  
Doaa Ibrahim ◽  
Alaa H. Sewid ◽  
Ahmed H. Arisha ◽  
Amir H. abd El-fattah ◽  
Adel M. Abdelaziz ◽  
...  
Keyword(s):  
Gene Expression ◽  
Body Weight ◽  
Campylobacter Jejuni ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Control Diet ◽  
Tight Junction Proteins ◽  
Expression Of Genes ◽  
Real Time Qpcr ◽  
Junction Proteins

Phytogenic feed additives have been gaining considerable interest due to their ability to improve gut health and thereby performance of broiler chickens. The impact of Glycyrrhiza glabra (licorice) extract (GE) on expression of genes coding for tight junction proteins and gut protection and Campylobacter jejuni colonization in broilers has not been discussed until now. Thus, the current study assessed the effective dose of GE for maximum growth in broiler chickens, clear-cut molecular mechanisms related to integrity and health of intestine, and controlling C. jejuni colonization. Over a 35-day feeding period, a total of 500 Ross broiler chicks were allocated to five groups; the first group was fed a control diet without GE and the second group to the fifth group were fed a control diet with GE (0.25, 0.5, 1, and 2 g/kg of diet); each group comprised 100 chicks with 10 replicates (10 birds/replicate). Birds fed GE had an improved body weight gain and feed conversion ratio. Furthermore, the highest body weight gain was observed in the group that received 1 g/kg of GE (P < 0.05). The expression of genes coding for tight junction proteins [occludin and junctional adhesion molecules (JAM)] was upregulated in all groups supplemented with GE. Moreover, birds fed 1 g/kg of GE exhibited the maximum gene expression of occludin and JAM [0.2 and 0.3 fold change, respectively (P < 0.05)]. In relation to enterocyte protective genes [glucagon-like peptide (GLP-2) and fatty acid-binding protein (FABP-6)], use of GE significantly upregulated expression of GLP-2 gene with 0.8 fold change in 2 g/kg of the GE supplemented group (P < 0.05) while the expression of FABP-6 gene was not affected by GE supplementation (P > 0.05). After challenge with C. jejuni, the expression of mucin (MUC-2) gene was upregulated and the inflammatory markers such as Toll-like receptors (TLR-4) and interleukin (IL-1β) were downregulated with increasing level of supplemented GE (P < 0.05). The mean log10 count of C. jejuni in cecal samples after 7 days post-infection by culture and real-time qPCR was decreased in groups fed GE in a dose-dependent manner (P < 0.05). In addition, the highest reduction of C. jejuni count in cecal samples by culture and real-time qPCR was observed in the group fed 2 g/kg of GE (2.58 and 2.28 log10 CFU/g, respectively). Results from this study suggested that G. glabra extract (1 g/kg) improved growth performance of broiler chickens, as well as influenced the maintenance of intestinal integrity and reduced C. jejuni shedding from infected birds.

scholarly journals Eicosapentaenoic acid actions on adiposity and insulin resistance in control and high-fat-fed rats: role of apoptosis, adiponectin and tumour necrosis factor-α

2007 ◽  
Vol 97 (2) ◽  
pp. 389-398 ◽  
Author(s):  
Patricia Pérez-Matute ◽  
Nerea Pérez-Echarri ◽  
J. Alfredo Martínez ◽  
Amelia Marti ◽  
María J. Moreno-Aliaga
Keyword(s):  
Gene Expression ◽  
Insulin Resistance ◽  
Body Weight ◽  
Adipose Tissue ◽  
Weight Gain ◽  
Body Weight Gain ◽  
Control Diet ◽  
Cafeteria Diet ◽  
High Fat ◽  
Beneficial Effects

n-3 PUFA have shown potential anti-obesity and insulin-sensitising properties. However, the mechanisms involved are not clearly established. The aim of the present study was to assess the effects of EPA administration, one of the n-3 PUFA, on body-weight gain and adiposity in rats fed on a standard or a high-fat (cafeteria) diet. The actions on white adipose tissue lipolysis, apoptosis and on several genes related to obesity and insulin resistance were also studied. Control and cafeteria-induced overweight male Wistar rats were assigned into two subgroups, one of them daily received EPA ethyl ester (1 g/kg) for 5 weeks by oral administration. The high-fat diet induced a very significant increase in both body weight and fat mass. Rats fed with the cafeteria diet and orally treated with EPA showed a marginally lower body-weight gain (P = 0·09), a decrease in food intake (P < 0·01) and an increase in leptin production (P < 0·05). EPA administration reduced retroperitoneal adipose tissue weight (P < 0·05) which could be secondary to the inhibition of the adipogenic transcription factor PPARγ gene expression (P < 0·001), and also to the increase in apoptosis (P < 0·05) found in rats fed with a control diet. TNFα gene expression was significantly increased (P < 0·05) by the cafeteria diet, while EPA treatment was able to prevent (P < 0·01) the rise in this inflammatory cytokine. Adiposity-corrected adiponectin plasma levels were increased by EPA. These actions on both TNFα and adiponectin could explain the beneficial effects of EPA on insulin resistance induced by the cafeteria diet.

scholarly journals Effects of multi-strain probiotic supplementation on intestinal microbiota, tight junctions, and inflammation in young broiler chickens challenged with Salmonella enterica subsp. enterica

2020 ◽  
Vol 33 (11) ◽  
pp. 1797-1808
Author(s):  
Chi Huan Chang ◽  
Po Yun Teng ◽  
Tzu Tai Lee ◽  
Bi Yu
Keyword(s):  
Gene Expression ◽  
Tight Junction ◽  
Intestinal Microbiota ◽  
Broiler Chickens ◽  
Infected Group ◽  
Tight Junction Proteins ◽  
Probiotic Supplementation ◽  
Mucin 2 ◽  
Ifn Γ ◽  
Junction Proteins

Objective: This study assessed the effects of probiotics on cecal microbiota, gene expression of intestinal tight junction proteins, and immune response in the cecal tonsil of broiler chickens challenged with Salmonella enterica subsp. enterica.Methods: One-day-old broiler chickens (n = 240) were randomly allocated to four treatments: negative control (Cont), multi-strain probiotic-treated group (Pro), Salmonella-infected group (Sal), and multi-strain probiotic-treated and Salmonella-infected group (ProSal). All chickens except those in the Cont and Pro groups were gavaged with 1×10<sup>8</sup> cfu/mL of S. enterica subsp. enterica 4 days after hatching.Results: Our results indicated that body weight, weight gain, and feed conversion ratio of birds were significantly reduced (p<0.05) by Salmonella challenge. Chickens challenged with Salmonella decreased cecal microbial diversity. Chickens in the Sal group exhibited abundant Proteobacteria than those in the Cont, Pro, and ProSal groups. Salmonella infection downregulated gene expression of Occludin, zonula occludens-1 (ZO1), and Mucin 2 in the jejunum and Occludin and Claudin in the ileum. Moreover, the Sal group increased gene expression of interferon-γ (IFN-γ), interleukin-6 (IL-6), IL-1β, and lipopolysaccharide-induced tumor necrosis factor-alpha factor (LITAF) and reduced levels of transforming growth factor-β4 and IL-10 compared with the other groups (p<0.05). However, chickens receiving probiotic diets increased Lactobacillaceae abundance and reduced Enterobacteriaceae abundance in the ceca. Moreover, supplementation with probiotics increased the mRNA expression of Occludin, ZO1, and Mucin 2 in the ileum (p<0.05). In addition, probiotic supplementation downregulated the mRNA levels of IFN-γ (p<0.05) and LITAF (p = 0.075) and upregulated IL-10 (p = 0.084) expression in the cecal tonsil.Conclusion: The administration of multi-strain probiotics modulated intestinal microbiota, gene expression of tight junction proteins, and immunomodulatory activity in broiler chickens.

scholarly journals Growth performance and carcass characteristics of finisher broiler chickens fed diet containing cooked cocoyam tubermeal

2020 ◽  
Vol 46 (4) ◽  
pp. 161-170
Author(s):  
B. C. Anyaegbu ◽  
D. N. Onunkwo ◽  
G. Igwe ◽  
J. Nathaniel ◽  
C. M. Nkwo
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Broiler Chicken ◽  
Body Weight Gain ◽  
Control Diet ◽  
Carcass Characteristics ◽  
Feed Conversion ◽  
Feeding Trial

This study was carried out to evaluate the growth performance and carcass characteristics of finisher broiler chickens fed diet containing cooked cocoyam tubermeal as energy source in place of maize. The proximate composition of cooked cocoyam tubermeal showed that it contained 13.11% moisture, 2.60% ash, 3.90% crude protein, 9.45% crude fiber, 1.85% ether extract and 70.13% NFE. In the finisher feeding trial, the cooked cocoyam tubermeal was used to replace maize at levels of 25%, 50%, 75% and 100% respectively in the control diet. Each finisher diet was fed to a group of 30 finisher broiler chicken for 4 weeks using completely randomized design. Each treatment was divided into 3 replicates of 10 broiler chicks each. The birds were kept on deep litter and were given feed and water ad-libitum. Parameters measured include ifinal body weight, body weight gain feed intake, feed conversion ratio, carcass characteristics and economics of production. In the finisher feeding trial, the feed intake of the finisher broilers on diets 2, 3 and 4 were similar and compared favourably with those on the control diet. The finisher group on 100% CCYM (diet 5) recorded the lowest feed intake possibly because of the dustiness of the feed. The body weight gain of the finisher broilers on diet 2 (25%) cooked cocoyam tubermeal compared favourably with the control group and was significantly (P<0.05) higher than those on other diets. It appeared that the finisher broilers could not tolerate high levels of cooked cocoyam meal due to dustiness of the feed and its anti-nutritional factors. The cost of production per kg finisher broilers was cheapest for diet 5(100% CCYM) (N356.72) and the costliest was for diet 3(50% CCYM) (N588.28). The internal organs expressed as percent of the live weight were not affected by the treatments. In terms of carcass characteristics, the finisher broilers on diet 2(25% CCYM) recorded the highest breast muscle which was significantly different (P<0.05) from other groups. There were no significant different (P>0.05) on percent back cut, drumstick, head, shank, thigh, neck and wings of the finisher broilers on all the treatments. There were no significant differences (P > 0.05) in percent dressed weight of the finisher broilers on cooked cocoyam based diets. The results of the trial have shown that cooking cocoyam corms (Xanthosoma sagittifolium) for 30 minutes was effective for reducing the cyanide oxalate, tannin, phytic acid and saponin content and that cooking did not affect the proximate composition of tannia. Cooked cocoyam tubermeal could be used to replace maize up to 25% in the diet of finisher broiler chicken without affecting body weight gain, feed intake and feed conversion ratio as indicated in this study.

scholarly journals Impacts of increasing challenge with Eimeria maxima on the growth performance and gene expression of biomarkers associated with intestinal integrity and nutrient transporters

2021 ◽  
Vol 52 (1) ◽  
Author(s):  
Po-Yun Teng ◽  
Janghan Choi ◽  
Yuguo Tompkins ◽  
Hyun Lillehoj ◽  
Woo Kim
Keyword(s):  
Gene Expression ◽  
Amino Acid ◽  
Tight Junction ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Nutrient Transporters ◽  
Tight Junction Proteins ◽  
Intestinal Integrity ◽  
Eimeria Maxima ◽  
Junction Proteins

AbstractThis study was conducted to investigate the impacts of graded severity of Eimeria maxima infection on the growth performance and intestine health of broiler chickens. Four different levels of E. maxima-challenged treatments were used, including a non-challenged control group, a low challenge (12 500 oocysts), a medium challenge (25 000 oocysts), and a high challenge dose (50 000 oocysts). There were eight replicate cages per treatment, with 12 birds in each cage, and chickens in the challenged groups orally received sporulated oocysts on day 14. Gastrointestinal permeability was measured by fluorescein isothiocyanate dextran at 5 days post-infection (dpi), whereas intestinal morphology and gene expression of nutrient transporters and tight junction proteins were determined at 6 dpi. The results demonstrate a linear reduction in growth performance, jejunal villus height, and jejunal integrity with graded challenge doses of E. maxima (P < 0.01). Moreover, linear regulation of nutrient transporters and tight junction proteins was a consequence of increasing Eimeria infection levels (P < 0.01). The linear increase of Claudin 1, cationic amino acid transporter, glucose transporter 1, and L-type amino acid transporter genes was associated with increased severity of coccidiosis (P < 0.01). Furthermore, expression of nutrient transporters located at the brush border membrane were down-regulated (P < 0.01) with increasing E. maxima inoculation dose. In conclusion, growth performance and key intestinal integrity biomarkers in broiler chickens were adversely influenced in a dose-dependent manner by E. maxima infection.

scholarly journals Dietary Encapsulated Essential Oils Improve Production Performance of Coccidiosis Vaccine-challenged Broiler Chickens

2020 ◽  
Author(s):  
Jeong-Woo Lee ◽  
Da-Hye Kim ◽  
Yoo Bhin Kim ◽  
Su-Been Jeong ◽  
Sungtaek Oh ◽  
...  
Keyword(s):  
Body Weight ◽  
Weight Gain ◽  
Essential Oils ◽  
Feed Intake ◽  
Broiler Chickens ◽  
Volatile Fatty Acids ◽  
Body Weight Gain ◽  
Control Diet ◽  
Vaccine Dose ◽  
Production Performance

The present study was conducted to evaluate encapsulated essential oils as an alternative anticoccidial in coccidiosis vaccine challenged broiler chickens. A total of 600 day-old male broiler chicks were provided with no-added corn-soybean meal-based control diet or diets that contained either salinomycin or EO at 60 and 120 mg per kg of diet. On day 21, half of the control groups were orally challenged with a coccidiosis vaccine at 25 times higher than the recommended vaccine dose. During 22 to 28 days (i.e., one-week post coccidiosis vaccine challenge), the challenged chickens had decrease (P &lt; 0.05) in body weight gain and feed intake but increase in feed conversion ratio compared with the non-challenged, na&iuml;ve control chickens. However, dietary EO significantly counteracted (P &lt; 0.05) coccidiosis vaccine-induced depression in body weight gain and feed intake. Increasing dietary EO linearly decreased (P &lt; 0.05) the concentrations of the volatile fatty acids. Dietary SAL and EO affected gut morphology in chickens at 20 days posthatch. Increasing dietary EO linearly (P = 0.073) increased serum catalase activity. Collectively, our study shows that dietary EO increased coccidiosis vaccine-induced growth depression and altered gut physiology in broiler chickens.

scholarly journals Intermittent feeding programme and addition of <i>Bacillus subtilis</i> based probiotics to the diet of growing broiler chickens: Influence on growth, hepatic enzymes and serum lipid metabolites profile

2013 ◽  
Vol 56 (1) ◽  
pp. 410-422 ◽  
Author(s):  
H. R. Aliakbarpour ◽  
M. Chamani ◽  
G. Rahimi ◽  
A. A. Sadeghi ◽  
D. Qujeq
Keyword(s):  
Body Weight ◽  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Serum Lipid ◽  
Broiler Chickens ◽  
Body Weight Gain ◽  
Control Diet ◽  
Intermittent Feeding ◽  
Lipid Metabolites ◽  
Ad Libitum

Abstract. This study was conducted to examine the effects of Intermittent feeding programme (IFP) and Bacillus subtilis-based probiotic (BSP) addition in diet on liver malic enzyme and isocitrate dehydrogenase activity, lipid metabolism and performance in broiler chickens. Five hundred and four one-day old male broiler chicks were randomly allocated in four experimental treatments (T1, T2, T3 and T4). Groups T1 (control diet) and T3 (Bacillus subtilis-based probiotic diet) were fed ad libitum, whereas groups T2 (control diet) and T4 (Bacillus subtilis-based probiotic diet) served as IFP from day 8 to the end of the experiment. The data on initial body weight, weekly feed consumption and body weight gain were recorded up to six weeks of age. Carcass composition, blood parameters and hepatic enzyme activity were measured at the end of the experiment. Although body weight gain was not significantly different among any of the treatments, the birds raised under IFP consumed significantly (P<0.05) lower feed (207 g) and utilized their feed more efficiently (1.78) than those of the control group fed ad libitum (1.84). Carcass weight as a percentage of live weight was not affected by probiotic supplementation on the diet, but IFP significantly reduced (P<0.05) broiler carcass weight. However, the liver malic and isocitrate dehydrogenase enzyme activity was not significantly different between IFP and BSP groups. All serum lipid metabolites concentration decreased (P<0.05) with probiotic treatment. It may be concluded that dietary supplementation with BSP may influence the pathway of fat metabolism through promotion and/or suppression of serum lipid metabolites.

Evidence that gene expression of ovarian follicular tight junction proteins is regulated in vivo and in vitro in cattle

2017 ◽  
Vol 95 (3) ◽  
pp. 1313 ◽  
Author(s):  
L. Zhang ◽  
L. F. Schütz ◽  
C. L. Robinson ◽  
M. L. Totty ◽  
L. J. Spicer
Keyword(s):  
Gene Expression ◽  
Tight Junction ◽  
Tight Junction Proteins ◽  
Junction Proteins

scholarly journals Effects of Lactobacillus reuteri and Streptomyces coelicolor on Growth Performance of Broiler Chickens

2021 ◽  
Vol 9 (6) ◽  
pp. 1341
Author(s):  
Sarayu Bhogoju ◽  
Collins N. Khwatenge ◽  
Thyneice Taylor-Bowden ◽  
Gabriel Akerele ◽  
Boniface M. Kimathi ◽  
...  
Keyword(s):  
Broiler Chickens ◽  
Lactobacillus Reuteri ◽  
Body Weight Gain ◽  
Streptomyces Coelicolor ◽  
Control Diet ◽  
Probiotic Bacteria ◽  
Feed Consumption ◽  
Feed Conversion ◽  
Wheat Middlings ◽  
Dietary Treatments

There are well documented complications associated with the continuous use of antibiotics in the poultry industry. Over the past few decades, probiotics have emerged as viable alternatives to antibiotics; however, most of these candidate probiotic microorganisms have not been fully evaluated for their effectiveness as potential probiotics for poultry. Recent evaluation of a metagenome of broiler chickens in our laboratory revealed a prevalence of Lactobacillus reuteri (L. reuteri) and Actinobacteria class of bacteria in their gastrointestinal tract. In this study Lactobacillus reuteri and Streptomyces coelicolor (S. coelicolor) were selected as probiotic bacteria, encapsulated, and added into broiler feed at a concentration of 100 mg/kg of feed. In an 8-week study, 240 one day-old chicks were randomly assigned to four dietary treatments. Three dietary treatments contained two probiotic bacteria in three different proportions (L. reuteri and S. coelicolor individually at 100 ppm, and mixture of L. reuteri and S. coelicolor at 50 ppm each). The fourth treatment had no probiotic bacteria and it functioned as the control diet. L. reuteri and S. coelicolor were added to the feed by using wheat middlings as a carrier at a concentration of 100 ppm (100 mg/kg). Chickens fed diets containing L. reuteri and S. coelicolor mixture showed 2% improvement in body weight gain, 7% decrease in feed consumption, and 6–7% decrease in feed conversion ratios. This research suggests that L. reuteri and S. coelicolor have the potential to constitute probiotics in chickens combined or separately, depending on the desired selection of performance index.

scholarly journals Fishmeal Replacement with Hermetia illucens Meal in Aquafeeds: Effects on Zebrafish Growth Performances, Intestinal Morphometry, and Enzymology

Fishes ◽  
2021 ◽  
Vol 6 (3) ◽  
pp. 28
Author(s):  
Baldassare Fronte ◽  
Rosario Licitra ◽  
Carlo Bibbiani ◽  
Lucia Casini ◽  
Mahanama De Zoysa ◽  
...  
Keyword(s):  
Body Weight ◽  
Body Weight Gain ◽  
Control Diet ◽  
Low Carbon ◽  
Replacement Rate ◽  
Hermetia Illucens ◽  
Fishmeal Replacement ◽  
Fish Intestine ◽  
Growth Performances ◽  
Intestinal Morphometry

Fishmeal (FM) is still the most important protein source in aquafeeds. However, due to the reduction of wild fish stocks used for FM production, its manufacturing it is now unsustainable. Insect meal represents a valid alternative to FM, due to the low carbon footprint of its production and its high nutritional value. The aim of this study was to investigate the potentials of replacing FM with black soldier fly (Hermetia illucens) meal (HIM) in aquafeeds, using zebrafish as the animal model. Four diets were formulated with increasing HIM/FM replacement rate (the control diet contained 20% FM). Mortality rates, growth performances, and feed consumptions were measured, and fish intestine samples were collected for histological and enzymatic analysis. After 49 days, all groups almost tripled their initial body weight (cumulative body weight gain ranged between 143.9 and 155.2 mg), and no statistically significant differences among treatments were observed in relation to growth performances and histological traits. Contrarily, trypsin, alkaline phosphatases, and alpha amylase–glucoamylase activities were significantly reduced when the FM replacement rate was increased. In conclusion, HIM may represent an alternative to FM, since no adverse effects were observed when it was included up to 20% in the zebrafish diet, even when replacing 100% of the FM.

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