scholarly journals Primary, Secondary Metabolites and Molecular Characterization of Hawthorn (Crataegus spp.) Genotypes

Agronomy ◽  
2020 ◽  
Vol 10 (11) ◽  
pp. 1731
Author(s):  
Aysen Gurlen ◽  
Muttalip Gundogdu ◽  
Goksel Ozer ◽  
Sezai Ercisli ◽  
Boris Duralija
Keyword(s):  
Citric Acid ◽  
Binding Sites ◽  
Morphological Characterization ◽  
Resolving Power ◽  
Crataegus Monogyna ◽  
The World ◽  
Citric Acid Content ◽  
Polymorphic Bands ◽  
First Time

In this study, the molecular, biochemical and agro-morphological characterization of genotypes belonging to hawthorn species collected from Bolu province of Turkey was performed. Inter-priming binding sites (iPBS) markers based on retrotransposons were used for the first time in the molecular properties of hawthorn genotypes in the world. The marker system provided very useful information for revealing the genetic variation of the genotypes. Six iPBS markers amplified 68 fragments, of which 65 were polymorphic (95.59%) with an average of 10.83 polymorphic bands per primer. The polymorphism and resolving power per primers ranged from 0.12 to 0.42 and from 0.78 to 8.11 with the average being 0.32 and 5.95, respectively. Pomological properties of Crataegus tanacetifolia, such as fruit pomology and core weight were determined to higher than those of Crataegus monogyna. Citric acid was determined as the most predominant organic acid, followed by malic and succinic acid in the genotypes of both species. The highest citric acid content (26.745 mg 100 g−1) was noted for 14BL09 genotype. The vit. C content was recorded ranging from 2.681 to 9.621 mg 100 g−1. Catechin, chlorogenic, caffeic and rutin contents were varied between 4.140–51.393 mg, 2.254–42.361 mg, 0.624–4.407 mg, and 1.241–10.029 mg per 100 g of fruits, respectively. As a result, it has been determined that twenty-five genotypes belonging to different hawthorn species are important genetic resources to be evaluated in horticultural breeding studies in terms of their physical and biochemical contents.

scholarly journals The crisis of content verbality in Internet discourse

2021 ◽  
pp. 224-228
Author(s):  
Марина Михайловна Молчанова ◽  
Патимат Абдулаевна Лекова
Keyword(s):  
Social Networks ◽  
Computer Technology ◽  
Linguistic Competence ◽  
The Internet ◽  
The World ◽  
Younger Generation ◽  
Media Texts ◽  
Active Visualization ◽  
First Time

Исследуется кризис вербальности контента в интернет-дискурсе, его активная визуализация, связанная с новыми открытиями в области компьютерных технологий. Цель работы - дать полиаспектную характеристику этому явлению и обозначить перспективы его влияния на речемыслительную деятельность подрастающего поколения, составляющего большую часть пользователей социальных сетей. Актуальность данной проблематики обусловлена обеспокоенностью филологической общественностью тем, что мир становится все менее лингвистическим. Новизна заключается в том, что впервые интернет-дискурс рассматривается в контексте кризиса вербальности контента. При таких темпах визуализации медиатекстов вполне вероятно обострение кризиса вербальности, последствия которого могут негативно сказаться на мыслетворческой деятельности у подрастающего поколения, которая возможна только при высокой языковой компетенции. The paper examines the crisis of content verbality in the Internet discourse, its active visualization associated with new discoveries in the field of computer technology. The aim of the work is to give a multi-aspect characterization of this phenomenon and outline the prospects of its influence on the speech-thinking activity of the younger generation, which makes up the majority of users of social networks. The relevance of this issue is due to the concern of the philological community that the world is becoming less and less linguistic. The novelty lies in the fact that for the first time Internet discourse is considered in the context of the crisis of content verbality. At such a rate of visualization of media texts, it is likely that the crisis of verbality will aggravate, the consequences of which can negatively affect the thinking activity of the younger generation, which is possible only with high linguistic competence.

scholarly journals Characterization of a profilin-like protein from Fasciola hepatica

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e10503
Author(s):  
Jessica Wilkie ◽  
Timothy C. Cameron ◽  
Travis Beddoe
Keyword(s):  
Fasciola Hepatica ◽  
Recombinant Expression ◽  
Tegument Proteins ◽  
Novel Treatments ◽  
The World ◽  
Eukaryotic Organisms ◽  
Novel Drug ◽  
First Time ◽  
Important Disease

Fasciola hepatica is the causative agent of fasciolosis, an important disease of humans and livestock around the world. There is an urgent requirement for novel treatments for F. hepatica due to increasing reports of drug resistance appearing around the world. The outer body covering of F. hepatica is referred to as the tegument membrane which is of crucial importance for the modulation of the host response and parasite survival; therefore, tegument proteins may represent novel drug or vaccine targets. Previous studies have identified a profilin-like protein in the tegument of F. hepatica. Profilin is a regulatory component of the actin cytoskeleton in all eukaryotic cells, and in some protozoan parasites, profilin has been shown to drive a potent IL-12 response. This study characterized the identified profilin form F. hepatica (termed FhProfilin) for the first time. Recombinant expression of FhProfilin resulted in a protein approximately 14 kDa in size which was determined to be dimeric like other profilins isolated from a range of eukaryotic organisms. FhProfilin was shown to bind poly-L-proline (pLp) and sequester actin monomers which is characteristic of the profilin family; however, there was no binding of FhProfilin to phosphatidylinositol lipids. Despite FhProfilin being a component of the tegument, it was shown not to generate an immune response in experimentally infected sheep or cattle.

scholarly journals A Novel Genotype and First Record of Trypanosoma lainsoni in Argentina

Pathogens ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 731
Author(s):  
Anahí G. Díaz ◽  
Paula G. Ragone ◽  
Fanny Rusman ◽  
Noelia Floridia-Yapur ◽  
Rubén M. Barquez ◽  
...  
Keyword(s):  
Host Range ◽  
First Record ◽  
Morphological Characterization ◽  
Pairwise Identity ◽  
Wild Mammals ◽  
Transmission Electron ◽  
Chaco Region ◽  
Veterinary Importance ◽  
First Time

Trypanosomes are a group of parasitic flagellates with medical and veterinary importance. Despite many species having been described in this genus, little is known about many of them. Here, we report a genetic and morphological characterization of trypanosomatids isolated from wild mammals from the Argentine Chaco region. Parasites were morphologically and ultrastructurally characterized by light microscopy and transmission electron microscopy. Additionally, 18s rRNA and gGAPDH genes were sequenced and analyzed using maximum likelihood and Bayesian inference. Morphological characterization showed clear characteristics associated with the Trypanosoma genus. The genetic characterization demonstrates that the studied isolates have identical sequences and a pairwise identity of 99% with Trypanosoma lainsoni, which belongs to the clade of lizards and snakes/rodents and marsupials. To date, this species had only been found in the Amazon region. Our finding represents the second report of T. lainsoni and the first record for the Chaco region. Furthermore, we ultrastructurally described for the first time the species. Finally, the host range of T. lainsoni was expanded (Leopardus geoffroyi, Carenivora, Felidae; and Calomys sp., Rodentia, Cricetidae), showing a wide host range for this species.

Morphological and molecular characterization of cucullanid nematodes including Cucullanus opisthoporus n. sp. in freshwater fish from the Brazilian Amazon

2016 ◽  
Vol 91 (6) ◽  
pp. 739-751 ◽  
Author(s):  
F.B. Pereira ◽  
J.L. Luque
Keyword(s):  
New Species ◽  
Freshwater Fish ◽  
Original Description ◽  
Morphological Characterization ◽  
Rrna Genes ◽  
28S Rrna ◽  
Type Specimens ◽  
Tail Tip ◽  
First Time

AbstractMolecular and morphological characterization of two species of Cucullanidae from freshwater fish in Brazil are provided, one of which represented an undescribed taxon. Cucullanus opisthoporus n. sp. was collected in Cichla melaniae from River Xingu, State of Pará, and in C. pinima from River Jamarí, State of Rondônia. Nematodes referable to Cucullanus grandistomis were collected in Oxydoras niger from River Xingu. The new species has an appendage in the tail tip, ventrally covered by small spines, which is an exclusive feature of Cucullanus tucunarensis. However, C. tucunarensis differs from C. opisthoporus n. sp. based upon the relative position of deirids and the excretory pore, which are more posterior from the oesophageal end in the new species. Observations of C. tucunarensis type specimens also revealed features that were wrongly or not reported in the original description. Type specimens of C. grandistomis were observed, although they were poorly preserved. After evaluation of newly collected specimens of C. grandistomis, features unreported in the original description were observed for the first time, including the presence of an intestinal caecum. Thus, C. grandistomis was transferred to Dichelyne. Sequences of the 18S and 28S rRNA genes revealed high genetic similarity between the specimens of C. opisthoporus n. sp. from the two different hosts as well as their genetic distance from Dichelyne grandistomis n. comb. Phylogenetic reconstructions using representatives of Cucullanidae suggested the artificiality of the current morphological system adopted to separate the genera, since most genera were not monophyletic, although the availability of genetic data is still fragmented.

An extraordinary new genus and three new species of Acostemmini (Hemiptera: Cicadellidae: Deltocephalinae) from Madagascar with comments on the morphology and classification of the tribe

Zootaxa ◽  
2012 ◽  
Vol 3209 (1) ◽  
pp. 28 ◽  
Author(s):  
J. N. ZAHNISER ◽  
M. W. NIELSON
Keyword(s):  
New Species ◽  
New Genus ◽  
Strong Support ◽  
Molecular Data ◽  
Morphological Characterization ◽  
New Combination ◽  
Speci Mens ◽  
Three New Species ◽  
First Time

The circumscription and morphological characterization of the deltocephaline tribe Acostemmini is revised based on dis-coveries of new and poorly known taxa from recent collections in Madagascar and from examination of the type speci-mens of Alocoelidia fulva Evans, Iturnoria insulana Evans, and Protonesis delegorguei Spinola. Alocoelidia Evans 1954,Caelidioides Signoret 1880, Iturnoria Evans 1954, and Protonesis Spinola 1850 were previously placed in Coelidiinaebut were later removed from the subfamily and until now have been unplaced to subfamily are placed here in Deltocepha-linae: Acostemmini, new placement. A new genus and species, Ikelibeloha cristata gen. n., sp. n., are described that havean unusually modified structure of the head and a large, crested pronotum. Molecular data (28S, Histone H3 genes) wereobtained for Ikelibeloha and Iturnoria, and analyzed with other members of Deltocephalinae. Results of the phylogeneticanalyses show strong support for the monophyletic clade ((Acostemma, Eryapus), (Ikelibeloha, Iturnoria)) and thus a re-lationship between previously described Acostemmini, Ikelibeloha, and Iturnoria has strong statistical support, and aclose relationship is inferred between these and the morphologically similar Alocoelidia, Caelidioides, and Protonesis.Two new species of Alocoelidia, A. maurae sp. n. and A. chasei sp. n. are described, and the genus is redescribed. Themale genitalia of Caelidioides tristis (Signoret), Iturnoria insulana, Alocoelidia fulva, and Acostemella rubra Evans areillustrated and described for the first time. The female genitalia of A. fulva, C. tristis and I. insulana are described and thefirst and second valvulae of C. tristis are illustrated. The habitus and face of Protonesis delegorguei are illustrated and thegenus is redescribed. The characters traditionally used to define Acostemmini are reviewed with notes on their known de-grees of variation, and characters that differentiate Acostemmini from the closely related tribe Stegelytrini are discussed.The type of Malagasiella minima Evans was also examined, and Malagasiella Evans, 1954 is considered a junior syn-onym of Doratulina Melichar, 1903 (Deltocephalinae: Stenometopiini), syn. n. giving the new combination Doratulina minima (Evans) comb. n.

scholarly journals Structure and distribution of glandular trichomes in three species of Bignoniaceae

2015 ◽  
Vol 45 (4) ◽  
pp. 347-354 ◽  
Author(s):  
Fabíola Fernandes Paiva de Castro FRÓES ◽  
Thália do Socorro Serra GAMA ◽  
Ana Carla FEIO ◽  
Diego DEMARCO ◽  
Ana Cristina Andrade de AGUIAR-DIAS
Keyword(s):  
Uniform Distribution ◽  
Glandular Trichomes ◽  
Morphological Characterization ◽  
Electron Microscopies ◽  
Nodal Region ◽  
Peltate Trichomes ◽  
First Time ◽  
Scanning Electron

Glandular trichomes play a major role in the morphological characterization of the Bignoniaceae. Due to their great diversity of forms and functions, this study aimed to inventory the glandular trichomes present in the aerial vegetative axis of Amphilophium magnoliifolium, Martinella obovata and Stizophyllum riparium, analyze their structure and register the participation of ants in these plants. Fresh samples from the nodal region, petiole and from medium to apical regions of the leaflet blade were fixed and processed according to usual methods in light and scanning electron microscopies. The glandular trichomes found were: peltate, capitate, stipitate, and patelliform/cupular. Peltate trichomes are the most abundant ones and present the most uniform distribution. Patelliform/cupular trichomes occur at specific regions, such as prophylls, leaflet blade and nodal regions. Martinella obovata is the only species that presents capitate and stipitate trichomes, which are widely distributed along the entire aerial vegetative axis. Ants were found in all species, mainly at nodal regions. The occurrence of the capitate-type trichome is reported for the first time to the genus.

scholarly journals Molecular and morphological characterization of the grapevine cultivars “Italia” in the Ica and Cañete valleys (Peru)

2019 ◽  
Vol 12 ◽  
pp. 01017
Author(s):  
K. Mendoza ◽  
M.R. Torres ◽  
G.A. Aliquo ◽  
J.A. Prieto ◽  
M. Grados ◽  
...  
Keyword(s):  
Simple Sequence Repeat ◽  
Parentage Analysis ◽  
Morphological Characterization ◽  
Variety Identification ◽  
Morphological Descriptors ◽  
Greenish Yellow ◽  
The World ◽  
Simple Sequence ◽  
Made In

The denomination of “Uva de Italia” or “Italia” is commonly used by growers to refer to a group of varieties used from the colonial times to making a brandy of Pisco with muscat aroma. Previous work have demonstrated that Uva de Italia corresponds in fact to the variety Muscat of Alexandria, a widely spread variety around the world and in South America. However, the distinction between “Italia Dorada” and “Italia Rosada” is usually made, in allusion to the color variations observed in the berries. Our aim was to characterize 5 samples collected in vineyards from the valleys of Ica and Cañete. The genotypes were identified using 13 molecular markers of nuclear simple sequence repeat, and 23 morphological descriptors according to OIV. Our results showed that four genotypes were identified as Muscat of Alexandria while the other corresponded to variety well-known in Argentina as Moscatel Rosado or Uva Pastilla in Chile respectively. Moscatel Rosado showed functionally female flowers, with variable berries size and color in the range from greenish yellow through pink. This is the first identification of Moscatel Rosado as a variety present in the Peruvian vineyards and would allow its use in the pisco industry with distinctive aromatic characteristics. Keywords: Italia, Muscat of Alexandria, Moscatel Rosado, variety identification, parentage analysis, microsatellites, Pisco.

Prevalence of Cryptosporidiosis in Ostriches from Central and South Parts of Iraq

2020 ◽  
Vol 44 (1) ◽  
pp. 63-67
Author(s):  
Al- Zubaidei H. Hamza
Keyword(s):  
Infection Rate ◽  
Morphological Characterization ◽  
Wide Spread ◽  
Traditional Methods ◽  
Fecal Samples ◽  
Cryptosporidium Spp ◽  
Significant Difference ◽  
June July ◽  
First Time

The prevalence of Ostriches cryptosporidiosis was determined for the first time in central and south parts of Iraq to study the effects of age, sex and months on the infection rate and to record the morphological characterization of Cryptosporidium spp in Ostriches. A total of 200 Ostriches fecal samples were examined by traditional methods for detection of the parasite. The total infection rate was 11% (22/200), and the highest infection rate was 12.26% (13/106) in chicks ( 9 months), while the lowest infection rate was 9.57% (9/94) recorded in the adult (> 9 Months). The study included seven provinces in the central and south parts of Iraq: Wasit, Baghdad, Babylon, Diyala, Karbala, Al-Najaf and Al-Qadisiyah. The highest infection rate was reported in Al-Najaf (23.53%) compared with the lowest rate (0%) in Wasit province. March reported an infection rate of 50%, while the lowest rate (0%) was registered during the summer months: June, July and August with a significant difference (P0.05) among months of the study. Morphologically, the study indicated the wide spread of Cryptosporidium parasites in Ostriches in both central and south area of Iraq

First molecular detection and characterization of Sarcocystis infection in naturally infected buffaloes, Brazil

2020 ◽  
Author(s):  
Luiza Pires Portella ◽  
Fagner D'ambroso Fernandes ◽  
Camila Encarnação Minuzzi ◽  
Juliana Felipetto Cargnelutti ◽  
Luis Fernando Vilani de Pelegrini ◽  
...  
Keyword(s):  
Microscopic Examination ◽  
Genomic Dna ◽  
Molecular Detection ◽  
Natural Infection ◽  
Sarcocystis Species ◽  
Sarcocystis Spp ◽  
The World ◽  
First Time ◽  
Pathogenic Effects

Sarcocystosis is a disease caused by varying Sarcocystis species infecting humans and animals. It is commonly found in ruminants causing pathogenic effects. Although the distribution of Sarcocystis can be found all over the world, the species infecting buffaloes in Brazil is still unknown. Through this study, we aim to estimate the molecular prevalence of natural infection with Sarcocystis spp. in buffaloes using molecular identification. In addition, phylogenetic analyzes were used for the first time to identify the different species of this protozoan infecting buffalo in the south of the country. Heart samples from 80 buffaloes were subjected to microscopic examination, followed by molecular analysis. Microcysts were present in 19/80 (23,75%) of the samples. Genomic DNA was extracted from the 19 isolates, all there were amplified DNA in the primer used in the study. Six readable sequences were obtained after sequencing of the samples in both the directions. In the present study all the sequenced samples indicated were of Sarcocystis levinei.

scholarly journals Purification of Hollow Sporopollenin Microcapsules from Sunflower and Chamomile Pollen Grains

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2094
Author(s):  
Jose Manuel Ageitos ◽  
Sandra Robla ◽  
Lorena Valverde-Fraga ◽  
Marcos Garcia-Fuentes ◽  
Noemi Csaba
Keyword(s):  
Drug Delivery ◽  
Pollen Grains ◽  
Morphological Characterization ◽  
Product Density ◽  
Low Protein ◽  
Pollen Types ◽  
Delivery Platform ◽  
Nanoporous Surface ◽  
First Time

Pollen grains are natural microcapsules comprised of the biopolymer sporopollenin. The uniformity and special tridimensional architecture of these sporopollenin structures confer them attractive properties such as high resistance and improved bioadhesion. However, natural pollen can be a source of allergens, hindering its biomedical applicability. Several methods have been developed to remove internal components and allergenic compounds, usually involving long and laborious processes, which often cannot be extended to other pollen types. In this work, we propose an abridged protocol to produce stable and pristine hollow pollen microcapsules, together with a complete physicochemical and morphological characterization of the intermediate and final products. The optimized procedure has been validated for different pollen samples, also producing sporopollenin microcapsules from Matricaria species for the first time. Pollen microcapsules obtained through this protocol presented low protein content (4.4%), preserved ornamented morphology with a nanoporous surface, and low product density (0.14 g/cm3). These features make them interesting candidates from a pharmaceutical perspective due to the versatility of this biomaterial as a drug delivery platform.

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