scholarly journals Herd-Level and Individual Differences in Fecal Lactobacilli Dynamics of Growing Pigs

Animals ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 113
Author(s):  
Emilia König ◽  
Virpi Sali ◽  
Paulina Heponiemi ◽  
Seppo Salminen ◽  
Anna Valros ◽  
...  

We studied the fecal lactobacilli count and species diversity of growing pigs along with immune parameters associated with intestinal lactobacilli. Thirty pigs categorized as small (S, n = 12) or large (L, n = 18) at birth were followed from birth to slaughter in two commercial herds, H1 and H2. Herds differed in terms of their general management. We determined sow colostrum quality, colostrum intake, piglet serum immunoglobulins, and pig growth. We took individual fecal samples from pigs in the weaning and finishing units. We studied lactobacilli count and identified their diversity with 16S PCR. Total lactobacilli count increased in H1 and decreased in H2 between samplings. Lactobacilli species diversity was higher in H1 in both fecal sampling points, whereas diversity decreased over time in both herds. We identified altogether seven lactobacilli species with a maximum of five (one to five) species in one herd. However, a relatively large proportion of lactobacilli remained unidentified with the used sequencing technique. Small pigs had higher lactobacilli counts in both herds but the difference was significant only in H2 (p = 0.01). Colostrum quality was numerically better in H1 than in H2, where colostrum intake tended to be associated with total lactobacilli count (p = 0.05).

Author(s):  
Galen Strawson

This chapter examines the difference between John Locke's definition of a person [P], considered as a kind of thing, and his definition of a subject of experience of a certain sophisticated sort [S]. It first discusses the equation [P] = [S], where [S] is assumed to be a continuing thing that is able to survive radical change of substantial realization, as well as Locke's position about consciousness in relation to [P]'s identity or existence over time as [S]. It argues that Locke is not guilty of circularity because he is not proposing consciousness as the determinant of [S]'s identity over time, but only of [S]'s moral and legal responsibility over time. Finally, it suggests that the terms “Person” and “Personal identity” pull apart, in Locke's scheme of things, but in a perfectly coherent way.


2020 ◽  
Author(s):  
HARZIKO

Language is a tool used to convey ideas, ideas, feelings, and thoughts to others. Language, which is an arbiter sound symbol system that is used by members of one community to work together, interact, and identify themselves. In other words, language can be a means to convey intent or purpose so that the desired thing is achieved. As a communication tool, language develops over time. Therefore, language will never die if its speakers still care and love for the language comparative linguistics as a study or study of language which includes the comparison of cognate languages or the historical development of a language. This research aims to elucidate the use of Indonesian language at students of Iqra Buru University with Comparative Analysis. The method used in this research is descriptive with a qualitative approach. Data collected by observation, interview, and documentation techniques. Sources of data in this study were 5 speakers 5 speakers language Analyzing the data by means of data selection, data classification, and data presentation. The results of the study stated that there were phonological differences, namely the Jamee vowel system contained / ɛ / and / ɔ /, while the vocal system was / a / and the consonant system was Jamee / ɣ / or / R /, while the language / r /, then the difference pronunciations include single vowels, for example makɛn, bɔRa in Jamee and eating, bara in Language used by iqra Rush university students. Keywords


2020 ◽  
Vol 7 (Supplement_1) ◽  
pp. S86-S86
Author(s):  
Ann F Chou ◽  
Yue Zhang ◽  
Makoto M Jones ◽  
Christopher J Graber ◽  
Matthew B Goetz ◽  
...  

Abstract Background About 30–50% of inpatient antimicrobial therapy is sub-optimal. Health care facilities have utilized various antimicrobial stewardship (AS) strategies to optimize appropriate antimicrobial use, improve health outcomes, and promote patient safety. However, little evidence exists to assess relationships between AS strategies and antimicrobial use. This study examined the impact of changes in AS strategies on antimicrobial use over time. Methods This study used data from the Veterans Affairs (VA) Healthcare Analysis & Informatics Group (HAIG) AS survey, administered at 130 VA facilities in 2012 and 2015, and antimicrobial utilization from VA Corporate Data Warehouse. Four AS strategies were examined: having an AS team, feedback mechanism on antimicrobial use, infectious diseases (ID) attending physicians, and clinical pharmacist on wards. Change in AS strategies were computed by taking the difference in the presence of a given strategy in a facility between 2012–2015. The outcome was the difference between antimicrobial use per 1000 patient days in 2012–2013 and 2015–2016. Employing multiple regression analysis, changes in antimicrobial use was estimated as a function of changes in AS strategies, controlling for ID human resources in and organizational complexity. Results Of the 4 strategies, only change in availability of AS teams had an impact on antimicrobial use. Compared to facilities with no AS teams at both time points, antibiotic use decreased by 63.9 uses per 1000 patient days in facilities that did not have a AS team in 2012 but implemented one in 2015 (p=0.0183). Facilities that had an AS team at both time points decreased use by 62.2 per 1000 patient days (p=0.0324). Conclusion The findings showed that AS teams reduced inpatient antibiotic use over time. While changes in having feedback on antimicrobial use and clinical pharmacist on wards showed reduced antimicrobial use between 2012–2015, the differences were not statistically significant. These strategies may already be a part of a comprehensive AS program and employed by AS teams. In further development of stewardship programs within healthcare organizations, the association between AS teams and antibiotic use should inform program design and implementation. Disclosures All Authors: No reported disclosures


1982 ◽  
Vol 72 (4) ◽  
pp. 709-716 ◽  
Author(s):  
Tan Keng-Hong ◽  
Lee Soo-Lam

AbstractDacus dorsalis Hend. infested eleven, D. cucurbitae Coq. five and D. umbrosus F. two of the eighteen common fruits and vegetables grown in Penang, West Malaysia. D. tau (Wlk.) infested bacang (Mangifera foetida), D. caudatus F. chilli (Capsicum annuum) and D. frauenfeldi Schin. water guava (Eugenia javanica), together with D. dorsalis. Pomelo (Citrus grandis) was found infested for the first time by D. cucurbitae. No flies were trapped using Capilure and trimedlure as baits. Cue-lure attracted D. caudatus, D. cucurbitae, D. frauenfeldi, D. occipitalis (Bez.) and D. tau. Methyl eugenol attracted D. dorsalis and D. umbrosus. Dorsalure was less attractive to D. caudatus and D. dorsalis than cue-lure and methyl eugenol, respectively, but it was equally attractive to D. frauenfeldi as cue-lure. Using traps baited with cue-lure or methyl eugenol in five ecosystems, the highest numbers of males of D. dorsalis, D. umbrosus, D. frauenfeldi and D. caudatus trapped were from a village, on a vegetable farm for D. cucurbitae, and D. occipitalis was only caught in a forest. Analysis showed that for each species of Dacus the difference between ecosystems was highly significant. The few examples caught in grassland were probably migrants.


2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Nicholas Chesnaye ◽  
Yvette Meuleman ◽  
Esther De Rooij ◽  
Friedo W Dekker ◽  
Marie Evans ◽  
...  

Abstract Background and Aims Differences between the sexes are apparent in the epidemiology of CKD. Cross-sectional studies show that women consistently report a poorer health-related quality of life (QoL) than men, however, longitudinal studies are lacking. Here we investigate the sex-specific evolution of QoL over time in advanced CKD. As a secondary aim, we explore the sex-specific determinants of QoL. Method EQUAL is an observational prospective cohort study in stages 4 and 5 CKD patients ≥65 years not on dialysis with an incident estimated glomerular filtration rate (eGFR) < 20 ml/min/1.73m². Data on QoL (measured using the RAND-36), clinical and demographic patient characteristics were collected between April 2012 and September 2020. QoL trajectories were modelled by sex using linear mixed models, and joint models were applied to deal with informative censoring. We followed patients until death or dialysis initiation. Results We included 5151 QoL measurements in 1416 patients over a total of 1986 person years of follow-up. Overall, the physical component summary (PCS) declined with 2.0 (95% CI 1.4-2.6) points and the mental component summary (MCS) by 2.4 (95% CI 1.8-3.0) points per year. Although women had overall lower QoL scores, figure 1 demonstrates that PCS and MCS declined more than twice as fast in men (PCS: 2.4 per year, 95% CI 1.7 – 3.1, MCS: 2.9 per year, 95% CI 2.2 – 3.6) compared with women (PCS: 1.1 per year, 95% CI -0.2 – 2.0, MCS: 1.5 per year, 95% CI 0.5 – 2.4). We identified a non-linear interaction effect between sex and eGFR levels on QoL, demonstrating a stronger negative effect of decreased eGFR on both PCS (p=0.02) and MCS (p=0.04) in men compared with women. Subsequent adjustment for renal decline attenuated the difference in rate of QoL decline between men and women (difference after adjustment; PCS: 1.1, 95% CI -0.1 – 2.2, MCS: 1.2, 95% 0.0 – 2.3). In univariable analyses, higher serum haemoglobin was more beneficial to QoL in men compared to women (p-value for interaction; PCS: p=0.03, MCS: p=0.01). Higher serum phosphate had a strong harmful effect on both PCS and MCS in men, but not in women (PCS & MCS: p<0.001). The presence of pre-existing diabetes had a negative effect on PCS and MCS in men, but to a lesser extent in women (PCS: p=0.02, MCS: p=0.01). Conclusion Despite the higher overall QoL reported by men, both their physical and mental QoL declined approximately twice as fast compared with women. The faster decline in men was mediated in part by their lower levels of renal function, which had a stronger impact on their QoL as compared with women. Furthermore, in exploratory analyses we identified that high levels of phosphate, low levels of haemoglobin, and pre-existing diabetes were more detrimental to QoL in men than in women.


2013 ◽  
Vol 48 (9) ◽  
pp. 1269-1274 ◽  
Author(s):  
Marcos Speroni Ceron ◽  
Vladimir de Oliveira ◽  
Paulo Alberto Lovatto ◽  
Marcos Martinez do Vale

The objective of this work was to determine the maintenance requirement and the deposition efficiency of lysine in growing pigs. It was used the incomplete changeover experimental design, with replicates over time. Twelve castrated pigs with average body weight (BW) of 52±2 kg were kept in metabolism crates with a controlled temperature of 22ºC. The diets were formulated to supply 30, 50, 60, and 70% of the expected requirements of standardized lysine, and provided at 2.6 times the energy requirements for maintenance. The trial lasted 24 days and was divided into two periods of 12 days: seven days for animal adaptation to the diet and five days for sample collection. The increasing content of lysine in the diet did not affect dry matter intake of the pigs. The amount of nitrogen excreted was 47% of the nitrogen intake, of which 35% was excreted through feces and 65% through urine. The estimated endogenous losses of lysine were 36.4 mg kg-1 BW0.75. The maintenance requirement of lysine for pigs weighing around 50 kg is 40.4 mg kg-1 BW0.75, and the deposition efficiency of lysine is 90%.


2011 ◽  
Vol 26 (4) ◽  
pp. 317-327 ◽  
Author(s):  
Valentín D. Picasso ◽  
E. Charles Brummer ◽  
Matt Liebman ◽  
Philip M. Dixon ◽  
Brian J. Wilsey

AbstractCropping systems that rely on renewable energy and resources and are based on ecological principles could be more stable and productive into the future than current monoculture systems with serious unintended environmental consequences such as soil erosion and water pollution. In nonagricultural systems, communities with higher species diversity have higher productivity and provide other ecosystem services. However, communities of well-adapted crop species selected for biomass production may respond differently to increasing diversity. Diversity effects may be due to complementarity among species (complementary resource use and facilitative interactions) or positive selection effects (e.g., species with higher productivity dominate the mixture), and these effects may change over time or across environments. Our goal was to identify the ecological mechanisms causing diversity effects in a biodiversity experiment using agriculturally relevant species, and evaluate the implications for the design of sustainable cropping systems. We seeded seven perennial forage species in a replicated field experiment at two locations in Iowa, USA, and evaluated biomass productivity of monocultures and two- to six-species mixtures over 3 years after the establishment year under management systems of contrasting intensity: one or three harvests per year. Productivity increased with seeded species richness in all environments, and the positive relationship did not change over time. Polyculture overyielding was due to complementarity among species in the community rather than to selection effects of individual species. Complementarity increased as a log-linear function of species richness in all environments, and this trend was consistent across years. Legume–grass facilitation may explain much of this complementarity effect. Although individual species with high biomass production had a major effect on productivity of mixtures, the species producing the highest biomass in monoculture changed over the years in most environments. Furthermore, transgressive overyielding was observed and was more prevalent in later years, in some environments. We conclude that choosing a single well-adapted species for maximizing productivity may not be the best alternative over the long term and that high levels of species diversity should be included in the design of productive and ecologically sound agricultural systems.


2004 ◽  
Vol 61 (1) ◽  
pp. 140-147 ◽  
Author(s):  
Alf Harbitz ◽  
Michael Pennington

Abstract The shortest sailing distance through n sampling points is calculated for simple theoretical sampling domains (square and circle) as well as for a rather irregular and concavely shaped real sampling domain in the Barents Sea. The sampling sites are either located at the nodes of a square grid (regular sampling) or they are randomly distributed. For n less than ten, the exact shortest sailing distance is derived. For larger n, a traveling salesman algorithm (simulated annealing) was applied, and its bias (distance from true minimum) was estimated based on a case where the true minimum distance was known. In general, the average minimum sailing distance based on random sampling was considerably shorter than for regular sampling, and the difference increased with sample size until an asymptotic value was reached at about n=60 for a square domain. For the sampling domain in the Barents Sea used for shrimp (Pandalus borealis) abundance surveys (n=118 stations), the cruise-track lengths based on random sampling were approximately normally distributed. The mean sailing distance was 18% shorter than the cruise track for regular sampling and the standard deviation equalled 2.6%.


Author(s):  
Mbarga Manga Joseph Arsene ◽  
Smolyakova Larisa Andreevna ◽  
Podoprigora Irina Viktorovna

Background: Antibiotic resistance is increasingly an issue in both human health and veterinary medicine. It is important to regularly assess the resistance of strains which have high potential to transmit this resistance. Numerous researches have shown so far that coli forms are part of these bacteria and coliforms from breeding environments (in particular poultry farming) are the most concerned because of their permanent exposure to antibiotics and adaptation that could result. Aim: This work was carried out to investigate the sensitivity to antibiotics of coliforms isolated from the shell of poultry eggs. Methods: A total of 191 egg samples (149 chicken eggs and 42 quail eggs) were collected in supermarket of the city of Moscow, coliforms bacteria were isolated the shells of these eggs and their sensitivity to antibiotics was achieved by employing modified Kirby-Bauer’s disc method. The results have been analysed and interpreted using the Guidelines of Ministry of health of Russian Federation. Results and Discussion: It turns out that, among the 191 samples studied only 16.2% (31) of the chicken eggs contained coli forms, 7.3% (14) contained mold and 76.4% (146) were sterile while 81% (34) of quail eggs were sterile, 19% (8) contained mold and none contained coliforms. Of the 60 bacteria isolated, 39 (65%) were resistant to at least one antibiotic and the highest MAR Index observed was 0.58 while the lowest was 0.08. Of the 720 anti biograms performed, we observed 557 (77.4%) cases of sensitivity, 72 (10.0%) intermediate cases and 91 (12.6%) cases of resistance. The highest resistances were observed on tetracycline (50%), amoxycillin (40%) and ampicillin (30%) while the weakest resistances were observed on trimethoprim (11.7%), cefazolin (10%), ciprofloxacin (5%) and 1.7% for ceftriazone, fosfomycin and nitrofurantoin. A comparison between the sensitivity of the isolates to Cefazolin and their sensitivity to Cefazolin + Clavulanic acid showed that the difference between these two antibiotics was highly significant (P = 0.00). Conclusion: Antibiotic resistance of coliform bacteria isolated was high, with the highest MAR Index observed equal to 0.58. The bio security relating to the use of antibiotics must be reinforced, better use of current known antibiotics is necessary to prolong their effectiveness over time.


2014 ◽  
Vol 31 (04) ◽  
pp. 1450030 ◽  
Author(s):  
CHENGWEN JIAO ◽  
WENHUA LI ◽  
JINJIANG YUAN

We consider online scheduling of unit length jobs on m identical parallel-batch machines. Jobs arrive over time. The objective is to minimize maximum flow-time, with the flow-time of a job being the difference of its completion time and its release time. A parallel-batch machine can handle up to b jobs simultaneously as a batch. Here, the batch capacity is bounded, that is b < ∞. In this paper, we provide a best possible online algorithm for the problem with a competitive ratio of [Formula: see text].


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