scholarly journals The Dairy Cow Slurry Composition Used as Organic Fertilizer Is Influenced by the Level and Origin of the Dietary Protein

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2812
Author(s):  
Fernando Vicente ◽  
Douâa Elouadaf ◽  
Alejandra Sánchez-Vera ◽  
Ana Soldado ◽  
Senén De La Torre-Santos ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Faba Bean ◽  
Organic Fertilizer ◽  
Latin Square ◽  
Nitrogen Excretion ◽  
Protein Levels ◽  
Lactating Cows ◽  
Silage Process ◽  
Slurry Composition ◽  
Low Nitrogen

Less than 30% of dairy cattle’s nitrogen ingested is retained in milk. Therefore, large amounts of nitrogen can be excreted in manure and urine with a potential environmental impact. In addition, some legume forages can be more susceptible to proteolysis during the silage process than grasses, and dairy cows fed these legume silages would excrete a larger quantity of nitrogen in slurry. The objectives of this work were to evaluate the amount of nitrogen excretion in dairy cows fed different protein levels and legume silages with a view to improve the slurry quality as a co-product that can be used as fertilizer. Two double 3 × 3 Latin square trials were carried out in order to study three different protein levels (high, medium, and low) and three different silages (grass, faba bean, and field pea). Dry matter intake, milk production, and composition were not affected by treatments. The excretion of ammonia-N in the urine was almost four times lower in the diet with the lowest protein level. The ammonia-N in the urine was twice as high with the pea silage than faba bean and grass silages. In conclusion, the diet containing 13% of protein meets the protein requirement for lactating cows producing 31 kg daily, with low nitrogen excretion in the urine, and the main pathway for the excretion of surplus nitrogen from legume silages is through urine and the metabolization of pea silage protein goes toward ammonia-N.

The effects of feeding frequency of concentrates and feeding sequence of hay on eating behavior, ruminal environment and milk production in dairy cows

1994 ◽  
Vol 74 (1) ◽  
pp. 103-113 ◽  
Author(s):  
G. K. Macleod ◽  
P. E. Colucci ◽  
A. D. Moore ◽  
D. G. Grieve ◽  
N. Lewis
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Eating Behavior ◽  
Milk Fat ◽  
Latin Square ◽  
Feeding Frequency ◽  
Daily Feeding ◽  
Lactating Cows ◽  
Rumen Metabolism

Two lactation trials were conducted with Holstein cows to assess the effects of varying the feeding frequency of concentrates, addition of long hay, and the sequence of hay and grain feeding on performance, rumen fermentation and eating behavior. Numbers of primiparous and multiparous cows assigned to repeated Latin square designs (4 × 4) were 8 and 12 for exp. 1 and 12 and 20 for exp. 2. Each experiment utilized four extra rumen-fistulated lactating cows to examine aspects of rumen metabolism. The four treatments applied in exp. 1 were (a) twice-daily feeding of concentrate, no hay; (b) thrice-daily feeding of concentrate, no hay; (c) hay offered 1 h before concentrate; and (d) hay offered 1 h after concentrate. Treatments in exp. 2 were similar, with the following exceptions: (b) concentrate six times daily; (c) hay 2 h before concentrate; and (d) hay 0.5 h after concentrate. In each treatment in both experiments, alfalfa silage was offered ad libitum. The average concentrate/forage ratio of the diets was 74:26 and 60:40 for exp. 1 and 2, respectively. In exp. 1, except for time spent eating and concentration of rumen propionate (increased with addition of 2 kg hay d−1, P < 0.05), treatments had no effect on dry matter intake, milk production and rumen metabolism. In exp. 2, inclusion of hay in the diet (3 kg d−1) increased DM intake (P < 0.001) and milk (P < 0.05), protein and lactose (P < 0.01) yields but decreased milk-fat test (P < 0.01). Hay also increased the ruminal molar proportions of propionate, butyrate (P < 0.05) and valerate (P < 0.001) and decreased acetate (P < 0.05), isobutyrate and isovalerate (P < 0.001). As in exp. 1, rumen ammonia N and pH were not affected by treatments. The effects of hay inclusion in the diet in exp. 2 are consistent with the high quality of hay used, which had a lower fiber content than the silage. Key words: Feeding frequency, feeding sequence, lactating dairy cows

Abomasally infused SFA with varying chain length differently affect milk production and composition and alter hepatic and mammary gene expression in lactating cows

2020 ◽  
Vol 124 (4) ◽  
pp. 386-395
Author(s):  
D. E. Rico ◽  
J. E. Parales ◽  
B. A. Corl ◽  
A. Lengi ◽  
P. Y. Chouinard ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Chain Length ◽  
Milk Production ◽  
Milk Fat ◽  
Latin Square ◽  
Adenosine Monophosphate ◽  
Lactating Cows ◽  
Stearoyl Coa Desaturase ◽  
Fat Yield ◽  
Fat Supplements

AbstractThe aim of the present study was to compare the effects of post-ruminally infused fat supplements, varying in fatty acid (FA) chain length, on animal performance, metabolism and milk FA. Eleven multiparous Holstein dairy cows were used in a replicated incomplete 3 × 3 Latin square design with 7-d periods, separated by 7-d washouts. Treatments were administered as abomasal infusions of enrichments providing 280 g/d of FA: (1) palmitic acid (98·4 % 16 : 0; PA), (2) caprylic and capric acids (56·2 % 8 : 0, 43·8 % 10 : 0; medium-chain TAG (MCT)) and (3) stearic acid (99·0 % 18 : 0; SA). Relative to PA, SA decreased the efficiency of fat-corrected milk production, which was associated with a tendency for higher DM intake and lower FA absorption with SA, whereas MCT was not different from PA for these variables. Milk fat concentration and yield were increased by PA relative to SA, but only fat yield tended to be greater relative to MCT. Relative to PA, MCT increased milk fat concentration of FA < 16 C, whereas SA increased FA > 16 C. Expression of mammary stearoyl-coA desaturase 1 was lower with SA than with PA. Relative to PA, liver expression of adenosine monophosphate-activated protein kinase-1 and pyruvate kinase was increased with MCT, whereas expression of these genes tended to be increased by SA. The mechanism of increased fat secretion with PA does not seem to be related to a modulation of the expression of lipogenesis-related genes, but rather to increased substrate availability as reflected by milk FA profile.

scholarly journals Biomarker of Aflatoxin Ingestion: 1H NMR-Based Plasma Metabolomics of Dairy Cows Fed Aflatoxin B1 with or without Sequestering Agents

Toxins ◽  
2018 ◽  
Vol 10 (12) ◽  
pp. 545 ◽  
Author(s):  
Ibukun Ogunade ◽  
Yun Jiang ◽  
James Adeyemi ◽  
Andre Oliveira ◽  
Diwakar Vyas ◽  
...  
Keyword(s):  
Dairy Cows ◽  
1H Nmr ◽  
Aflatoxin B1 ◽  
Operating Characteristic ◽  
Plasma Concentrations ◽  
Area Under The Curve ◽  
Latin Square ◽  
Control Treatment ◽  
Lactating Cows ◽  
Saccharomyces Cerevisiae Fermentation Product

The study applied 1H NMR-based plasma metabolomics to identify candidate biomarkers of aflatoxin B1 (AFB1) ingestion in dairy cows fed no sequestering agents and evaluate the effect of supplementing clay and/or a Saccharomyces cerevisiae fermentation product (SCFP) on such biomarkers. Eight lactating cows were randomly assigned to 1 of 4 treatments in a balanced 4 × 4 Latin square design with 2 squares. Treatments were: control, toxin (T; 1725 µg AFB1/head/day), T with clay (CL; 200 g/head/day), and CL with SCFP (CL + SCFP; 35 g of SCFP/head/day). Cows in T, CL, and CL + SCFP were dosed with AFB1 from d 26 to 30. The sequestering agents were top-dressed from d 1 to 33. On d 30 of each period, 15 mL of blood was taken from the coccygeal vessels and plasma samples were prepared by centrifugation. Compared to the control, T decreased plasma concentrations of alanine, acetic acid, leucine, arginine and valine. In contrast, T increased plasma ethanol concentration 3.56-fold compared to control. Treatment with CL tended to reduce sarcosine concentration, whereas treatment with CL + SCFP increased concentrations of mannose and 12 amino acids. Based on size of the area under the curve (AUC) of receiver operating characteristic and fold change (FC) analyses, ethanol was the most significantly altered metabolite in T (AUC = 0.88; FC = 3.56); hence, it was chosen as the candidate biomarker of aflatoxin ingestion in dairy cows fed no sequestering agent.

scholarly journals Replacement of soybean meal by cottonseed meal in diets based on spineless cactus for lactating cows

2009 ◽  
Vol 38 (10) ◽  
pp. 1995-2000 ◽  
Author(s):  
Fabiana Maria da Silva ◽  
Marcelo de Andrade Ferreira ◽  
Adriana Guim ◽  
Ricardo Alexandre Silva Pessoa ◽  
Luiz Henrique dos Santos Gomes ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Cottonseed Meal ◽  
Milk Yield ◽  
Soybean Meal ◽  
Latin Square ◽  
Live Weight ◽  
Experimental Period ◽  
Neutral Detergent Fiber ◽  
Lactating Cows ◽  
Sorghum Silage

The objective of this study was to evaluate the replacement of soybean meal by cottonseed meal in the diet of dairy cows fed diets based on spineless cactus. Five Girolando lactating cows were used, with average live weight of 490 kg and average production of 11.5 kg of milk/day, distributed in a 5 × 5 Latin square design (5 animals, 5 treatments and 5 experimental periods). Each experimental period lasted 15 days, 10 days being for the adaptation of the animals to the diet and 5 days for data collection. The experimental diet consisted of spineless cactus (53%), sorghum silage (32%) and concentrate (15%). The cottonseed meal replaced 0, 25, 50, 75 and 100% of soybean meal in the concentrate. The intake, milk yield and composition were evaluated. The nutrients intake and digestibility were not affected by the treatments, with an average of 15.55 and 56.05; 13.8 and 59.31, 0.37 and 49.40, 5.32 and 30.95, 1.79 and 48.14; 9.94 and 54.31, 4.43 kg/day and 80.99%, for the dry matter, organic matter, ether extract, neutral detergent fiber, crude protein, total carbohydrates and non-fibrous carbohydrates, respectively. The total digestible nutrients were not affected (average of 8.30 kg/day). Similarly, the milk yield and composition, fat corrected milk yield (4%), lactose, total solids, fat and protein were not affected by replacement (11.56, 11.41 kg milk/day and 4.45, 12.75, 3.95 and 3.42%, respectively). Recommended the replacement of soybean meal by cottonseed meal for low production dairy cows.

scholarly journals Metabolic and Productive Response and Grazing Behavior of Lactating Dairy Cows Supplemented with High Moisture Maize or Cracked Wheat Grazing at Two Herbage Allowances in Spring

Animals ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 919
Author(s):  
Verónica M. Merino ◽  
Lorena Leichtle ◽  
Oscar A. Balocchi ◽  
Francisco Lanuza ◽  
Julián Parga ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Milk Production ◽  
Milk Fat ◽  
Dry Matter ◽  
Body Condition Score ◽  
Latin Square ◽  
Ruminal Fermentation ◽  
High Moisture ◽  
Plasma Urea ◽  
Grazing Behavior

The aim was to determine the effect of the herbage allowance (HA) and supplement type (ST) on dry matter intake (DMI), milk production and composition, grazing behavior, rumen function, and blood metabolites of grazing dairy cows in the spring season. Experiment I: 64 Holstein Friesian dairy cows were distributed in a factorial design that tested two levels of daily HA (20 and 30 kg of dry matter (DM) per cow) and two ST (high moisture maize (HMM) and cracked wheat (CW)) distributed in two daily rations (3.5 kg DM/cow/day). Experiment II: four mid-lactation rumen cannulated cows, supplemented with either HMM or CW and managed with the two HAs, were distributed in a Latin square design of 4 × 4, for four 14-d periods to assess ruminal fermentation parameters. HA had no effect on milk production (averaging 23.6 kg/day) or milk fat and protein production (823 g/day and 800 g/day, respectively). Cows supplemented with CW had greater protein concentration (+1.2 g/kg). Herbage DMI averaged 14.17 kg DM/cow.day and total DMI averaged 17.67 kg DM/cow.day and did not differ between treatments. Grazing behavior activities (grazing, rumination, and idling times) and body condition score (BCS) were not affected by HA or ST. Milk and plasma urea concentration increased under the high HA (+0.68 mmol/L and +0.90 mmol/L, respectively). Cows supplemented with HMM had lower milk and plasma urea concentrations (0.72 mmol/L and 0.76 mmol/L less, respectively) and tended (p = 0.054) to have higher plasma β-hydroxybutyrate. Ruminal parameters did not differ between treatments.

scholarly journals PSII-13 Effect of corn stover silages inoculated with effective microorganisms (EM®) on digestibility of sheeps

2020 ◽  
Vol 98 (Supplement_4) ◽  
pp. 408-410
Author(s):  
Manuel Pérez- ◽  
Claudia C Márquez-Mota ◽  
Atmir Romero-Pérez ◽  
Jonathan Avilés-Nieto ◽  
Luis Corona
Keyword(s):  
Corn Stover ◽  
Latin Square ◽  
Ruminal Fermentation ◽  
Effective Microorganisms ◽  
Cane Molasses ◽  
Milk Whey ◽  
Corn Stover Silage ◽  
Silage Process ◽  
Male Sheep

Abstract The objective of the study was to evaluate the effect of corn stover silages with two sources of energy (cane molasses or milk whey) and EM (effective microorganisms, 1 mL/kg DM) on the digestibility of nutrients and rumen fermentation. We hypothesized that inoculation with EM (lactobacillus spp and Rhodopseudomona palustris and Saccharomyces cerevisiae) in the silage process of corn stover coud improve the in vivo digestibility and ruminal fermentation in sheep. Six male sheep with cannula in the rumen were used in a 6 x 6 Latin square design. Experimental periods consisted of 11 d for diet adjustment followed by 5 d for collection. The animals were housed in metabolic cages, with a harness to collect faeces individually. Diets consisted of concentrate (corn grain, soybean meal, mineral salt) 55% and corn stover 45% dry basis. The treatments were: CS-AMW= Corn stover with acid milk whey, CS-CM= corn stover with sugar cane molasses, SIL-AMW= corn stover silage with acid milk whey, SIL-CM= corn stover silage with cane molasses, SIL-AMW-EM= corn stover silage with acid milk whey and EMand SIL-CM-EM= Corn stover silage with cane molasses and EM. The results were analyzed with PROC MIXED procedures of SAS. The inoculation of corn stover with EM increased (P &lt; 0.05) the content of CP in SIL-AMW-EM and SIL-CM-EM respect CS-CM (Table 1). SIL-CM-EM increase (P &lt; 0.05) the ruminal N-NH3 and pH (Table 2) but no effect was observed for digestibility (Table 3). However, the SIL-AMW improved (5.8%, P &lt; 0.05) the OM digestion compared with CS-AMW. A diet with 45% corn stover silage with EM was not sufficient to improve in vivo digestibility of nutrients in sheep. This project was supported by UNAM,DGAPA, PAPIIT (IT202120)

scholarly journals Characterization of Short-Term Heat Stress in Holstein Dairy Cows Using Altered Indicators of Metabolomics, Blood Parameters, Milk MicroRNA-216 and Characteristics

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 722
Author(s):  
Jang-Hoon Jo ◽  
Jalil Ghassemi Nejad ◽  
Dong-Qiao Peng ◽  
Hye-Ran Kim ◽  
Sang-Ho Kim ◽  
...  
Keyword(s):  
Heat Stress ◽  
Dairy Cows ◽  
Milk Yield ◽  
Milk Fat ◽  
Blood Parameters ◽  
Negative Effects ◽  
Short Term ◽  
Lactating Cows ◽  
Humidity Index ◽  
Holstein Dairy Cows

This study aims to characterize the influence of short-term heat stress (HS; 4 day) in early lactating Holstein dairy cows, in terms of triggering blood metabolomics and parameters, milk yield and composition, and milk microRNA expression. Eight cows (milk yield = 30 ± 1.5 kg/day, parity = 1.09 ± 0.05) were homogeneously housed in environmentally controlled chambers, assigned into two groups with respect to the temperature humidity index (THI) at two distinct levels: approximately ~71 (low-temperature, low-humidity; LTLH) and ~86 (high-temperature, high-humidity; HTHH). Average feed intake (FI) dropped about 10 kg in the HTHH group, compared with the LTLH group (p = 0.001), whereas water intake was only numerically higher (p = 0.183) in the HTHH group than in the LTLH group. Physiological parameters, including rectal temperature (p = 0.001) and heart rate (p = 0.038), were significantly higher in the HTHH group than in the LTLH group. Plasma cortisol and haptoglobin were higher (p < 0.05) in the HTHH group, compared to the LTLH group. Milk yield, milk fat yield, 3.5% fat-corrected milk (FCM), and energy-corrected milk (ECM) were lower (p < 0.05) in the HTHH group than in the LTLH group. Higher relative expression of milk miRNA-216 was observed in the HTHH group (p < 0.05). Valine, isoleucine, methionine, phenylalanine, tyrosine, tryptophan, lactic acid, 3-phenylpropionic acid, 1,5-anhydro-D-sorbitol, myo-inositol, and urea were decreased (p < 0.05). These results suggest that early lactating cows are more vulnerable to short-term (4 day) high THI levels—that is, HTHH conditions—compared with LTLH, considering the enormous negative effects observed in measured blood metabolomics and parameters, milk yield and compositions, and milk miRNA-216 expression.

scholarly journals Short-Term Variations of C18:1 Trans Fatty Acids in Plasma Lipoproteins and Ruminal Fermentation Parameters of Non-Lactating Cows Subjected to Ruminal Pulses of Oils

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 788
Author(s):  
Einar Vargas-Bello-Pérez ◽  
Juan J. Loor ◽  
Philip C. Garnsworthy
Keyword(s):  
Fatty Acids ◽  
Trans Fatty Acids ◽  
Latin Square ◽  
Density Lipoprotein ◽  
Plasma Lipoproteins ◽  
Ruminal Fermentation ◽  
Short Term ◽  
Lactating Cows ◽  
Fermentation Parameters ◽  
Partially Hydrogenated Vegetable Oil

The objective of this study was to evaluate short-term variations of trans fatty acids (TFA) in plasma lipoproteins and ruminal fermentation parameters of non-lactating cows subjected to ruminal pulses of vegetable oils. Three non-lactating, non-pregnant Holstein cows, each with a ruminal cannula, were arranged in a 3 × 3 Latin square design with three-day pulsing periods and four-day washout intervals between treatments. Cows were treated with single ruminal pulses of: (1) control (skimmed milk (SM); 500 mL); (2) soybean oil (SO; 250 g/d in 500 mL of SM) and (3) partially-hydrogenated vegetable oil (PHVO; 250 g/d in 500 mL of SM). Time changes after infusion in TFA contents were only observed for plasma C18:1 trans-4, trans-5 and trans-12, and high-density lipoprotein fraction C18:1 trans-9. After ruminal pulses, concentration of acetate decreased linearly; molar concentrations of propionate and valerate increased linearly; molar concentrations of butyrate and isovalerate changed quadratically and were greater at 1 h than at other times. There was an accumulation of several C18:1 TFA in plasma and lipoproteins, especially on the third day of pulsing. Overall, naturally occurring C18:1 TFA isomers (produced during ruminal biohydrogenation of SO) and preformed TFA (supplied by PHVO) elicited differential TFA partitioning and transport in plasma and lipoproteins.

319 Predicting Urinary Nitrogen Excretion from Milk Urea Nitrogen of Fresh Forage or Total Mixed Ration Fed Dairy Cows: A Meta-analysis

2021 ◽  
Vol 99 (Supplement_3) ◽  
pp. 171-171
Author(s):  
Matthew R Beck ◽  
Cameron Marshall ◽  
Konagh Garrett ◽  
Andrew P Foote ◽  
Ronaldo Vibart ◽  
...  
Keyword(s):  
Dairy Cows ◽  
Meta Analysis ◽  
Live Weight ◽  
Predictive Ability ◽  
Nitrogen Excretion ◽  
Good Precision ◽  
Urea Nitrogen ◽  
Total Mixed Ration ◽  
Milk Urea ◽  
Milk Urea Nitrogen

Abstract Urine nitrogen excretion (g/d; UN) represent a significant environmental impact for both confinement feeding and pastoral based dairy systems. It is difficult to measure UN directly due to animal handling and labor requirements, especially in forage based production systems. The currently available milk urea nitrogen (MUN) equations have been shown to overestimate UN excretion of grazing dairy cows compared with an equation using urinary creatinine and UN concentration, indicating that diet may alter the relationship between MUN and UN. This potential was explored using data retrieved (treatment means: n = 69 and 27 for fresh forage [FF] and total mixed ration [TMR] fed cattle, respectively) from the literature and new data obtained from dairy cows fed FF (n = 15) in metabolism crates was used to test the new equations. The TMR data from literature was further split into a training set (to develop the model; n = 53) and a test set (to validate the model; n = 16). There was an interaction for diet type (P &lt; 0.01) where UN (g/d) = 0.023 × MUN (mg/dL) × live-weight (kg, LW) for TMR fed cattle, (similar to a pre-established equation); however, UN (g/d) = 0.015 × MUN × LW for FF fed cattle. For FF based equations, the New MUN equation and the creatinine equation showed good precision and accuracy (Lin’s CCC = 0.79 and 0.74, respectively) and adequate predictive ability (RMSEP = 29.8 and 35.9, respectively). The new MUN equation for TMR fed cattle showed excellent accuracy and precision (Lin’s CCC = 0.87) with good predictive ability (RMSEP = 24.3) for UN excretion (observed mean = 216.5 g/d). The new equations generated during this meta-analysis provide promising predictive ability of UN excretion, which can be used for management considerations, future research, and policy making.

Supplementary feeding of dairy cows on continuously stocked pasture

1984 ◽  
Vol 103 (1) ◽  
pp. 161-170 ◽  
Author(s):  
P. G. Jennings ◽  
W. Holmes
Keyword(s):  
Dairy Cows ◽  
Latin Square ◽  
Live Weight ◽  
Metabolizable Energy ◽  
Total Output ◽  
Control Group ◽  
Stocking Rate ◽  
High Quality ◽  
Treatment Groups ◽  
Stocking Rates

SummaryTwo experiments were conducted with milking cows on continuously stocked perennial ryegrass pastures. In each a control group, T0, received 1 kg/day of a concentrate supplement and treatment groups T1 and T2 received 4 kg (Expt 1) or 5 kg/day (Expt 2) of a low quality T1 or a high quality T2 concentrate. In Expt 1 treatments were applied continuously for 14 weeks to a total of 30 cows. In Expt 2 a Latin square design for 9 weeks was conducted with 18 cows. The stocking rate of the pasture declined from 9·6 to 5·1 cows per ha (mean 6·7 cows/ha) from May to August (Expt 1) and was maintained at 3 cows/ha in August-October (Expt 2).Supplements increased total intakes by 0·92 and 0·77 kg organic matter (OM)/kg OM supplied in the concentrates respectively for Expts 1 and 2. Milk yields increased by 0·6 and 0·5 kg/kg concentrate supplied and supplemented cows showed small increases in live weight. Differences in lactation milk yield just approached significance. Grazing times were only slightly reduced by supplements and bite sizes were lower than normal. There was no important difference in animal performance between the two concentrates. The total output from the pasture was 19·6t milk and 115 GJ of utilized metabolizable energy per hectare.Reasons for the high supplementary effect of the concentrates and its implications for stocking rates are discussed.

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