scholarly journals Modulation of Intestinal Histology by Probiotics, Prebiotics and Synbiotics Delivered In Ovo in Distinct Chicken Genotypes

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3293
Author(s):  
Monika Bogusławska-Tryk ◽  
Ewa Ziółkowska ◽  
Anna Sławińska ◽  
Maria Siwek ◽  
Joanna Bogucka
Keyword(s):  
Small Intestine ◽  
Lactococcus Lactis ◽  
Broiler Chickens ◽  
Intestinal Morphology ◽  
Intestinal Histology ◽  
In Ovo ◽  
Final Body Weight ◽  
Sterile Saline ◽  
Lactococcus Lactis Subsp ◽  
Bioactive Substance

The aim of the study was to determine the effect of probiotics, prebiotics and synbiotics administered in ovo on selected morphological parameters of the small intestine (duodenum, jejunum, ileum) in broiler chickens (Ross 308) and native chickens (Green-legged Partridge, GP). On the 12th day of embryonic development (the incubation period), an aqueous solution of a suitable bioactive substance was supplied in ovo to the egg’s air cell: probiotic—Lactococcus lactis subsp. cremoris (PRO), prebiotic—GOS, galacto-oligosaccharides (PRE) or symbiotic—GOS + Lactococcus lactis subsp. cremoris (SYN). Sterile saline was injected into control (CON) eggs. After hatching, the chicks were placed in pens (8 birds/pen, 4 replicates/group) and housed for 42 days. On the last day of the experiment, all birds were individually weighed and slaughtered. Samples for histological analysis were taken directly after slaughter from three sections of the small intestine. In samples from the duodenum, jejunum and ileum, the height and width of the intestinal villi (VH) were measured and their area (VA) was calculated, the depth of the intestinal crypts (CD) was determined, the thickness of the muscularis was measured and the ratio of the villus height to the crypt depth (V/C) was calculated. On the basis of the obtained data, it can be concluded that the applied substances administered in ovo affect the production parameters and intestinal morphology in broiler chickens and GP. The experiment showed a beneficial effect of in ovo stimulation with a prebiotic on the final body weight of Ross 308 compared to CON, while the effect of the administered substances on the intestinal microstructure is not unequivocal. In GP, the best effect in terms of villi height and V/C ratio was found in the in ovo synbiotic group. Taking into account the obtained results, it can be concluded that chickens of different genotypes react differently to a given substance; therefore, the substances should be adapted to the genotype.

Effect of in ovo injected prebiotics and synbiotics on the caecal fermentation and intestinal morphology of broiler chickens

2017 ◽  
Vol 57 (9) ◽  
pp. 1884 ◽  
Author(s):  
D. Miśta ◽  
B. Króliczewska ◽  
E. Pecka-Kiełb ◽  
V. Kapuśniak ◽  
W. Zawadzki ◽  
...  
Keyword(s):  
Lactococcus Lactis ◽  
Broiler Chickens ◽  
Intestinal Morphology ◽  
Jejunal Mucosa ◽  
In Ovo ◽  
Lactococcus Lactis Subsp ◽  
Crypt Depth ◽  
Molar Proportion ◽  
Depth Ratio ◽  
And Performance

Manipulations of the intestinal microbiota composition may improve the health and performance of chickens. In ovo technology allows the administration of a bioactive substance to enter directly into the incubating egg. The objective of the present study was to investigate the effect of in ovo administered prebiotics or synbiotics on microbial activity products in the caeca and the development of the small intestine of broiler chickens. Ross 308 male chickens hatched from eggs injected in ovo with prebiotics or synbiotics were used in this study. Five experimental groups were formed: C (Control) – injected with 0.9% NaCl, the Pre-1 and Pre-2 groups – injected with prebiotics: inulin or Bi2tos, respectively, and the Syn-1 and Syn-2 groups – injected with synbiotics: inulin with Lactococcus lactis subsp. lactis IBB SL1 or Bi2tos with Lactococcus lactis subsp. cremoris IBB SC1, respectively. At the age of 7, 21 and 35 days, 10 chicks of each group were randomly selected, weighed and slaughtered, and the jejunal samples were collected for histological examinations, whereas caecal samples were collected to analyse the end products of microbial fermentation. Synbiotic treatment increased bodyweight, as observed in the Syn-1 group (P < 0.05). The propionate molar proportion was highest in the groups treated with synbiotics, especially in the Syn-1 group (P < 0.01). Furthermore, the molar proportion of acetate was also lowest in the Syn-1 group (P < 0.05). In ovo synbiotics treatment increased the villus length : crypt depth ratio in the jejunal mucosa, which might improve nutrient absorption and contribute to the increased weight of chickens. These effects suggest that the in ovo administration of synbiotics may be an effective method to increase bodyweight, improve the short-chain fatty acid caecal profile and increase the villus length : crypt depth ratio in the jejunal mucosa. These effects were more pronounced in the Syn-1 group than the Syn-2 group.

scholarly journals Effects of Selected Prebiotics or Synbiotics Administered in ovo on Lymphocyte Subsets in Bursa of the Fabricius, Thymus, and Spleen in Non-Immunized and Immunized Chicken Broilers

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 476
Author(s):  
Marianna Szczypka ◽  
Agnieszka Suszko-Pawłowska ◽  
Maciej Kuczkowski ◽  
Michał Gorczykowski ◽  
Magdalena Lis ◽  
...  
Keyword(s):  
Immune System ◽  
Lactococcus Lactis ◽  
Blood Cells ◽  
Broiler Chickens ◽  
Lymphocyte Subsets ◽  
Double Positive ◽  
In Ovo ◽  
Lactococcus Lactis Subsp ◽  
Egg Incubation ◽  
Stimulation Of

The effects of in ovo-delivered prebiotics and synbiotics on the lymphocyte subsets of the lymphoid organs in non-immunized 7-day-old broiler chickens and in non-immunized, sheep red blood cells (SRBC)-immunized, and dextran (DEX)-immunized 21- and 35-day-old birds were studied. The substances were injected on the 12th day of egg incubation: Prebiotic1 group (Pre1) with a solution of inulin, Prebiotic2 group (Pre2) with a solution of Bi2tos (non-digestive transgalacto-oligosaccharides), Synbiotic1 group (Syn1) with inulin and Lactococcus lactis subsp. lactis IBB SL1, and Synbiotic2 group (Syn2) with Bi2tos and Lactococcus lactis subsp. cremoris IBB SC1. In 7-day-old chicks, a decrease in T splenocytes was noticed in all groups. The most pronounced effect in 21- and 35-day-old birds was an increase in TCRγδ+ cells in Syn1 and Syn2 groups. A decrease in bursal B cells was observed in DEX-immunized Pre1 group (21-day-old birds), and in the Syn1 group in non-immunized and SRBC-immunized 35-day-old birds. An increase in double-positive lymphocytes was observed in Pre1 (35-day-old birds) and Pre2 (immunized 21-day-old birds) groups. In Pre1 and Syn1 groups (21- and 35-day-old), an increase in B splenocytes and a decrease in T splenocytes were observed. We concluded that Syn1 was the most effective in the stimulation of the chicken immune system.

scholarly journals Adding wheat and rapeseed meal to corn-soy diets affects intestinal morphology and nutrient digestibility in broilers

2021 ◽  
Vol 50 (6) ◽  
Author(s):  
E. Amirahmadi ◽  
A.R. Safamehr ◽  
A. Nobakht ◽  
Y. Mehmannavaz
Keyword(s):  
Small Intestine ◽  
Essential Oil ◽  
Broiler Chickens ◽  
Protein Digestibility ◽  
Rapeseed Meal ◽  
Nutrient Digestibility ◽  
Intestinal Morphology ◽  
Thyme Essential Oil ◽  
Randomized Design ◽  
Enzyme Supplementation

This study was conducted to investigate the effects of thyme essential oil and enzyme supplementation on rations with two different energy (wheat or corn) and protein resources (soy or rapeseed meals) on gut morphology and protein digestibility of broiler chickens. Three hundred eighty-four male Ross-308 broiler chickens were used with a 23 factorial arrangement with treatments according to a completely randomized design. The morphological examinations of the intestine were carried out on 1-cm long excised segments from duodenum, jejunum and ileum. Adding wheat to the diet increased small intestine length in birds compared to other feedstuffs (P <0.05). Duodenum crypt depth and jejunum villi length were also higher in groups fed with rations containing wheat (P <0.05). Groups that received thyme essential oil and enzyme supplementation had increased length of the duodenum (P <0.05). However, the two- and three-factor interaction effects were not significant. Protein digestibility was not affected by treatment (P >0.05). Feeding wheat to broiler chickens may increase the surface area for nutrient absorption in the small intestine as compensation for the anti-nutritive activity of non-starch polysaccharides in wheat-based diets. Wheat based diets are expected to be acceptable for broilers when supplemented with appropriate enzymes or thyme essential oil.

scholarly journals PSXIII-23 Dietary glutamine, glutamate, and aspartate supplementation improves morphology and intercellular junction of small intestine in piglets

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 472-474
Author(s):  
Jing Wang ◽  
Bi E Tan ◽  
Jianjun Li ◽  
Ming Qi ◽  
Wenkai Ren ◽  
...  
Keyword(s):  
Small Intestine ◽  
Basal Diet ◽  
Low Energy ◽  
Intestinal Morphology ◽  
Control Group ◽  
Mrna Levels ◽  
Villus Height ◽  
Final Body Weight ◽  
Weaning Stress ◽  
Low Energy Diet

Abstract Weaning-stress decreases the digestive and absorptive capacity of small intestine in piglets, resulting in reduction in energy intake for intestine cells and defects in epithelial structure. As glutamine (Gln), glutamate (Glu), and aspartate (Asp) are major energy sources for small intestine; thus, this study was conducted to test the hypotheses that supplementation with Gln, Glu, and Asp in diets will improve the intestinal morphology and tight junction in weaning piglets. 198 weaned piglets were assigned to the following treatments: i) Control (Basal diet + 1.59% L-Alanine); ii) T1 (Basal diet + 1% L-Glutamine + 0.5% L-Glutamate + 0.1% L-Aspartate); iii) T2 (Low energy diet + 1% L-Glutamine + 0.5% L-Glutamate + 0.1% L-Aspartate). The small intestinal samples were obtained on 5 or 21-day-post-weaning. The results showed that basal dietary supplementation with Gln, Glu, and Asp in basal diet improved the final body weight (BW), average daily gain (ADG) of piglets on 21-day of post-weaning. Supplementation with Gln, Glu, and Asp in diet with low energy decreased the villus height and crypt depth in ileum of piglets on 5-day-post-weaning, but increased villus height and goblet cell number in jejunum on 21-day post-weaning compared with those in control group. On 5-day-post-weaning, high mRNA levels of voltage-gated potassium (Kv) 1.1 in ileum and Kv 1.5 in jejunum were observed in T 1 and T 2 groups, respectively. Other indicators were higher than those in control piglets on day 5 or 21 post-weaning, including protein abundances of claudin-1, laudin-3 and occluding, and the percentage of proliferating cell nuclear antigen (PCNA)-positive cells in jejunum and ileum in T 1 or T 2 groups. Collectively, these findings indicated that Gln, Glu, and Asp can alleviate the intestinal barrier injury in piglets induced by weaning stress even under low energy diet.

Essential fatty acid deficiency in mice impairs lactose digestion

2008 ◽  
Vol 295 (3) ◽  
pp. G605-G613 ◽  
Author(s):  
S. Lukovac ◽  
E. L. Los ◽  
F. Stellaard ◽  
E. H. H. M. Rings ◽  
H. J. Verkade
Keyword(s):  
Small Intestine ◽  
Fatty Acid ◽  
Mrna Expression ◽  
Essential Fatty Acid ◽  
Intestinal Mucosa ◽  
Intestinal Morphology ◽  
Lactase Activity ◽  
Intestinal Histology ◽  
Fat Malabsorption ◽  
Small Intestinal

Essential fatty acid (EFA) deficiency in mice induces fat malabsorption. We previously reported indications that the underlying mechanism is located at the level of the intestinal mucosa. We have investigated the effects of EFA deficiency on small intestinal morphology and function. Mice were fed an EFA-deficient or control diet for 8 wk. A 72-h fat balance, the EFA status, and small intestinal histology were determined. Carbohydrate absorptive and digestive capacities were assessed by stable isotope methodology after administration of [U-13C]glucose and [1-13C]lactose. The mRNA expression and enzyme activity of lactase, and concentrations of the EFA linoleic acid (LA) were measured in small intestinal mucosa. Mice fed the EFA-deficient diet were markedly EFA-deficient with a profound fat malabsorption. EFA deficiency did not affect the histology or proliferative capacity of the small intestine. Blood [13C6]glucose appearance and disappearance were similar in both groups, indicating unaffected monosaccharide absorption. In contrast, blood appearance of [13C]glucose, originating from [1-13C]lactose, was delayed in EFA-deficient mice. EFA deficiency profoundly reduced lactase activity (−58%, P < 0.01) and mRNA expression (−55%, P < 0.01) in mid-small intestine. Both lactase activity and its mRNA expression strongly correlated with mucosal LA concentrations ( r = 0.77 and 0.79, respectively, P < 0.01). EFA deficiency in mice inhibits the capacity to digest lactose but does not affect small intestinal histology. These data underscore the observation that EFA deficiency functionally impairs the small intestine, which in part may be mediated by low LA levels in the enterocytes.

scholarly journals Effects of Probiotics, Prebiotics and Synbiotics Injected in Ovo on the Microstructure of the Breast Muscle in Different Chicken Genotypes

Animals ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2944
Author(s):  
Karolina Stasiak ◽  
Anna Slawinska ◽  
Joanna Bogucka
Keyword(s):  
Broiler Chickens ◽  
Fibre Diameter ◽  
Pectoral Muscle ◽  
General Purpose ◽  
Muscle Fibres ◽  
Histopathological Changes ◽  
In Ovo ◽  
Fibre Density ◽  
Genotype Group ◽  
Bioactive Substance

The aim of the study was to analyse the effect of probiotics, prebiotics and synbiotics injected in ovo on day 12 of embryonic development on the microstructure of the superficial pectoral muscle (musculus pectoralis superficialis) from 42-day-old chickens of different genotypes: broilers (Ross 308) and general-purpose type (green-legged partridge (GP) chickens Zk-11, native chickens). Incubated eggs were divided into four groups (each genotype separately) depending on the substance injected in ovo: normal saline (C, control); Lactococcus lactis subsp. cremoris (PRO); galactooligosaccharides, GOS (PRE) or GOS + L. lactis (SYN). After hatching, chicks were placed in eight replicated pens (four pens/genotype group). There were eight birds per pen. In total, 64 birds were used in the experiment. Birds were slaughtered at the age of 42 days, and samples of superficial pectoral muscles were taken for analysis. The microstructure of the pectoral muscles was evaluated using the cryosectioning (frozen tissue sectioning) technique and staining with haematoxylin and eosin. Statistical analysis revealed that the in ovo injection of probiotics, prebiotics and synbiotics had no significant effect on the diameter of muscle fibres from chickens of the two genotypes. The number of fibres in the muscles from green-legged partridge chickens was about three-fold higher than the fibre density in the muscles from broiler chickens, with the fibre diameter being two-fold smaller. This fact may indicate a greater tenderness of meat from GP chickens compared to the meat from Ross 308 broilers. In the case of broilers, a prebiotic (GOS) was the most effective bioactive substance in reducing the number of histopathological changes. Considering muscles from GP chickens, the number of normal fibres was highest in birds treated with the probiotic. These findings indicate that the microstructural features of pectoral muscles depend not only on the type of the injected bioactive substance but also on the genotype of chickens.

scholarly journals In ovo Administration of Nucleosides Improved the Performance, Apparent Metabolizable Energy and Gut Development in Broiler Chickens

2020 ◽  
Vol 7 ◽  
Author(s):  
Marappan Gopi ◽  
Villavan Manojkumar ◽  
Ashok Kumar Verma ◽  
Putan Singh ◽  
Jaydip Jaywant Rokade ◽  
...  
Keyword(s):  
Growth Performance ◽  
Plasma Protein ◽  
Broiler Chickens ◽  
Metabolizable Energy ◽  
Intestinal Morphology ◽  
Gut Development ◽  
In Ovo ◽  
Plasma Protein Concentration ◽  
Apparent Metabolizable Energy ◽  
Phosphate Buffered Saline

An in ovo study on the effect of the administration of a combination of nucleosides (25, 50, and 100 mg/egg) on hatchability, growth performance, energy metabolizability, and intestinal morphology in broilers was carried out. Four hundred eighty (480) fertile eggs were divided into four groups (in four replicates each having 30 eggs). On the 18th days of incubation of the eggs, candling was carried out and the fertile eggs were selected and given one of the four in ovo administrations. Group one served as control and was injected with phosphate-buffered saline (PBS). The other groups were given in ovo administration of nucleosides (25, 50, and 100 mg/egg) at 100 μl through the yolk sac route, and chicks of respective groups were hatched out. Among the experimental groups, the hatchability was comparable; however, the hatchability was affected in the group injected with a higher level of nucleosides at 100 mg/egg. The hatched out chicks from higher doses of nucleosides (50 and 100 mg) had higher body weight (BW) (P &lt; 0.05) than the control. Higher energy metabolizability (%) was observed in nucleoside-injected groups. Plasma protein concentration was higher in groups administered with nucleosides (50 and 100 mg). Histologically, the intestinal villi length was maximum in 100 mg-injected group followed by 50 and 25 mg. Relative expression of homeobox (Cdx) in the jejunum was significantly (P &lt; 0.05) upregulated in all the injected groups at 3, 7, and 14 days of age. Nucleoside-administered groups had better performance, energy metabolizability, and intestinal morphology. Among the experimental groups, the administration of nucleosides at 50 mg/egg resulted in higher growth performance, plasma protein, intestinal surface, and villi development in broiler chickens.

scholarly journals Effects of Dietary Ginsenoside Rg1 Supplementation on Growth Performance, Gut Health, and Serum Immunity in Broiler Chickens

2021 ◽  
Vol 8 ◽  
Author(s):  
Zehe Song ◽  
Kaihuan Xie ◽  
Yunlu Zhang ◽  
Qian Xie ◽  
Xi He ◽  
...  
Keyword(s):  
Body Weight ◽  
Growth Performance ◽  
Broiler Chickens ◽  
Basal Diet ◽  
Intestinal Morphology ◽  
Ginsenoside Rg1 ◽  
Gut Health ◽  
Villus Height ◽  
Final Body Weight ◽  
Crypt Depth

The restriction and banning of antibiotics in farm animal feed has led to a search for promising substitutes for antibiotics to promote growth and maintain health for livestock and poultry. Ginsenoside Rg1, which is one of the most effective bioactive components in ginseng, has been reported to have great potential to improve the anti-inflammatory and anti-oxidative status of animals. In this study, 360 Chinese indigenous broiler chickens with close initial body weight were divided into 5 groups. Each group contained 6 replicates and each replicate had 12 birds. The experimental groups were: the control group, fed with the basal diet; the antibiotic group, fed basal diet + 300 mg/kg 15% chlortetracycline; and three Rg1 supplementation groups, fed with basal diet + 100, 200, and 300 mg/kg ginsenoside Rg1, respectively. The growth performance, immune function, and intestinal health of birds were examined at early (day 1–28) and late (day 29–51) stages. Our results showed that dietary supplementation of 300 mg/kg ginsenoside Rg1 significantly improved the growth performance for broilers, particularly at the late stage, including an increase in final body weight and decrease of feed conversion ratio (P &lt; 0.05). Additionally, the integrity of intestinal morphology (Villus height, Crypt depth, and Villus height/Crypt depth) and tight junction (ZO-1 and Occludin), and the secretion of sIgA in the intestine were enhanced by the supplementation of Rg1 in chicken diet (P &lt; 0.05). The immune organ index showed that the weight of the thymus, spleen, and bursa was significantly increased at the early stage in ginsenoside Rg1 supplementation groups (P &lt; 0.05). Our findings might demonstrate that ginsenoside Rg1 could serve as a promising antibiotic alternative to improve the growth performance and gut health for broiler chickens mainly through its amelioration of inflammatory and oxidative activities.

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