Resveratrol Protects against Restraint Stress Effects on Stomach and Spleen in Adult Male Mice

The objectives were to investigate whether restraint stress (which is known as a mixture of psychologic and physical stress) exerts negative effects on the stomach and spleen, and whether the phenolic compound resveratrol (RES) exerts any protective roles. Fifty adult male mice were divided into five groups, with 10 mice per group as follows: control (C), restraint stress (RS), RS with vehicle (RS + V), RS with 2 mg/kg of resveratrol (RS + 2 mg RES), and RS with 20 mg/kg of resveratrol (RS + 20 mg RES). Mice were restrained in conical centrifuge tubes for 4 h daily to establish the RS model. RS + 2 mg RES, RS + 20 mg RES, and RS + V groups were given an oral dose of resveratrol or vehicle for 15 consecutive days, while the control group was not exposed to restraint stress. Herein, we showed that restraint stress decreased body weight and food and water consumption in stressed groups RS and RS + V compared to controls, while the groups treated with resveratrol showed improvements. Moreover, restraint stress caused acute damage to the morphology of gastric cells and reduced the quantitative distribution of parietal cells along with their decreased size and diameter, pointing to gastritis or ulcer. Furthermore, the antibody against the apoptosis-inducing factor (AIF) was highly attached in the RS groups. Splenic size, weight, and length were also greatly augmented in the stressed groups compared to the controls, while these phenomena were not observed in the RS + 2 mg RES group. Our findings proved significant ameliorating effects of resveratrol against restraint stress in adult male mice.

Download Full-text

Acute and subchronic co-administrations to cadmium, diazinon and selenium induce apparent osteoporotic symptoms in adult male rats

Biologia ◽

10.2478/s11756-014-0436-y ◽

2014 ◽

Vol 69 (10) ◽

Author(s):

Hana Ďúranová ◽

Monika Martiniaková ◽

Ivana Boboňová ◽

Radoslav Omelka ◽

Robert Stawarz ◽

...

Keyword(s):

Adult Male ◽

Osteoporotic Fractures ◽

Bone Diseases ◽

Male Rats ◽

Control Group ◽

Femoral Bone ◽

Negative Effects ◽

Young Males ◽

Male Wistar Rats ◽

Group B

AbstractCadmium (Cd) and diazinon (DZN) are known to be environmental risk factors for various bone diseases including osteoporosis. Selenium (Se), an essential constituent of many antioxidant enzymes, has in higher concentrations negative effects on the bone. The present study was aimed to investigate possible changes in femoral bone of adult male rats after their acute and subchronic exposures to Cd, DZN and Se. A total of 30 male Wistar rats were randomized into three experimental groups. The rats in the group A (4-months-old) were injected intraperitoneally with a mixture of 2 mg CdCl2 kg−1, 20 mg DZN kg−1 and 2 mg Na2SeO3 kg−1 body weight and killed 36 h after xenobiotics had been injected. In the group B, young males (1-month-old) were administered with a combination of 30 mg CdCl2 L−1, 40 mg DZN L−1 and 5 mg Na2SeO3 L−1 in their drinking water, for 90 days. Ten 4-months-old males without toxicant supplementation served as a control group (C). After treatment period, detailed histological analysis of femoral bone was performed in each group. Our results revealed apparent osteoporotic symptoms (resorption lacunae, osteoporotic fractures) in rats from groups A and B. Moreover, histomorphometrical evaluation showed reduced bone vascularization (constricted primary osteons’ vascular canals and Haversian canals) and weakness mechanical properties of bones (smaller size of the secondary osteons) in these rats in comparison with those of the control group. Our study demonstrates for the first time that acute and subchronic co-administrations to Cd, DZN and Se induce evident manifestation characteristics of osteoporosis in male rats.

Download Full-text

Correlation between Antistress and Hepatoprotective Effects of Schisandra Lignans Was Related with Its Antioxidative Actions in Liver Cells

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/161062 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 7

Author(s):

Hao-Jie Pu ◽

Yun-Feng Cao ◽

Rong-Rong He ◽

Zhi-Long Zhao ◽

Jin-Hui Song ◽

...

Keyword(s):

Free Radicals ◽

Liver Damage ◽

Restraint Stress ◽

Liver Cells ◽

Protective Effects ◽

Male Mice ◽

Stress Effects ◽

Restraint Tube ◽

Hepatoprotective Effects ◽

The Relationship

The present study was conducted to investigate the relationship between the anti-stress and hepato-protective effects of Schisandra Lignans Extract (SLE) on stress-induced liver damage. Seven weeks old male mice were fixed in a restraint tube for 18 h to induce liver damage. SLE was orally administered to animals for 5 days at dosages of 100 and 200 mg/kg/day before exposed to restraint stress. Oral administration of SLE significantly reduced restraint-induced liver damage in experimental animal. SLE was further found to significantly alleviate the provocation of corticosterone in stressed mice. SLE also significantly decreased oxidative damage and increased anti-oxidative capability of liver cells by preventing the over production and accumulation of free radicals. In conclusion, the protective effects of SLE on stress-induced liver damage were confirmed, and the correlation between hepatoprotective and anti-stress effects of schisandra lignans was possible related to its alleviation on the malignant effects of stressors for bio-homeostasis, such as balance of oxidation and reduction in cells.

Download Full-text

Low semen quality and adverse histological changes in testes of adult male mice treated with bee venom (Apis mellifera)

Open Veterinary Journal ◽

10.4314/ovj.v11i1.11 ◽

2021 ◽

Vol 11 (1) ◽

pp. 70-79

Author(s):

Sassia O. Regeai ◽

Salma A. Abusrer ◽

Naema S. Shibani

Keyword(s):

Male Infertility ◽

Adult Male ◽

Semen Quality ◽

Sperm Count ◽

Bee Venom ◽

Reproductive Status ◽

Seminiferous Tubules ◽

Control Group ◽

Male Mice ◽

Histological Changes

Background: Male infertility has been on the rise since the past seven decades. Recently, in Libya, bee venom therapy (BVT) has become a popular method among alternative healthcare practitioners for treating male infertility. However, a literature search did not find any published studies that investigated the use of BVT for infertility treatment. Aim: To investigate the effect of bee venom on the male reproductive status through measurements of semen quality parameters and testicular histological changes in adult male mice. Methods: A total of 48 male mice were randomly divided into three experimental groups (which were subdivided into two subgroups with eight mice each) as follows: control, bee venom sting (BVS), and bee venom injection (BVI). The normal control subgroup mice were not subjected to any treatment, while the vehicle control subgroup mice were injected (i.p.) with 200 μl of 0.9% saline solution. In the BVS-treated subgroups, each mouse was stung by one live bee for five times (BVS-5) or seven times (BVS-7) every third day for 2 or 3 weeks. While each mouse in the BVI-treated subgroups received 23 μg/kg in a dose volume of 200 μl BVIs (i.p.) for five times (BVI-5) or seven times (BVI-7) every third day for 15 or 21 days. Results: The findings of this study showed that repeated bee venom treatment by sting or injection to adult male mice resulted in a significant decline in testosterone levels, sperm count, sperm motility, and a very significant increase in the percentage of abnormal sperm morphology; also, there were harmful testicular histological changes in the structural organization of seminiferous tubules and degenerative changes in the germinal epithelium compared to control group. Conclusion: The results of this study provide evidence for the low semen quality and adverse testicular histological changes in male mice treated with bee venom. Hence, there is a desperate need for educating alternative healthcare practitioners and infertile couples about the harmful effects of BVT on reproductive status.

Download Full-text

Evaluation of response to restraint stress by salivary corticosterone levels in adult male mice

Journal of Veterinary Medical Science ◽

10.1292/jvms.15-0610 ◽

2016 ◽

Vol 78 (5) ◽

pp. 775-780 ◽

Cited By ~ 13

Author(s):

Masakatsu NOHARA ◽

Atsushi TOHEI ◽

Takumi SATO ◽

Hiromi AMAO

Keyword(s):

Adult Male ◽

Restraint Stress ◽

Male Mice

Download Full-text

Effects of Diabetes Mellitus on the Reproductive System of Adult Male Mice After One Cycle of Spermatogenesis

European Scientific Journal ESJ ◽

10.19044/esj.2021.v17n34p149 ◽

2021 ◽

Vol 17 (34) ◽

pp. 149

Author(s):

Kouassi Emile Bégbin ◽

N’Guessan Ernest Zougrou ◽

Georges Abizi ◽

Koffi Kouakou ◽

N’Takpé Emmanuel Jaurès Mangué

Keyword(s):

Diabetes Mellitus ◽

Adult Male ◽

Reproductive System ◽

Male Reproductive System ◽

Diabetic Group ◽

Seminiferous Tubules ◽

Control Group ◽

Male Mice ◽

Chronic Hyperglycemia ◽

Study Results

Background: Diabetes mellitus is a disease characterized by chronic hyperglycemia. Poor management of hyperglycemia leads to complications that can reduce quality of life. Diabetes mellitus has been associated with damage to the reproductive system. The present study examines effects of diabetes mellitus on the reproductive system in alloxan-induced diabetic mice after one cycle of spermatogenesis. Materials and Methods: Twelve (12) mice divided into "control" and "diabetic" groups of six (6) animals each were used. Diabetes was induced in adult male mice by intraperitoneal injection with a single dose of 220 mg/kg body weight of alloxan. After 40 days, sperm density, morphology, and motility were assessed by standard methods. Serum levels of testosterone, FSH, and LH were measured. In addition, testes and epididymis were removed for histological study. Results: Highly significant (P < 0.001) reductions in serum testosterone, FSH, and LH levels, as well as normal and motile sperm counts of 21% and 34% respectively were observed in the diabetic group. The control group had three times more sperm than the diabetic group. The histological increased inter-tubular spaces, loss of interstitial tissue, degeneration of seminiferous tubules, and almost complete reduction of sperm count in the epididymal duct. Conclusion: Chronic hyperglycemia is therefore deleterious to the male reproductive system of mice. It leads to hypogonadism, which causes dysfunction of the male reproductive system, and can lead to infertility in men with diabetes mellitus.

Download Full-text

Effect of Cadmium Chloride on the Histological Structure of Lung in Adult Male Mice with and without Parsley Oil

Open Access Macedonian Journal of Medical Sciences ◽

10.3889/oamjms.2021.6207 ◽

2021 ◽

Vol 9 (A) ◽

pp. 676-679

Author(s):

Maha Alsammak

Keyword(s):

Body Weight ◽

Adult Male ◽

Cadmium Chloride ◽

Single Daily Dose ◽

Control Group ◽

Histological Structure ◽

Microscopical Examination ◽

Male Mice ◽

Daily Dose ◽

Intragastric Tube

This study is to detect the toxic effect of cadmium chloride on the histological structure of the lung and the effect of parsley oil to amilorate these changes In this experiement 40 adult male mice were divided into four groups. Goup A (control group) in this group animals were injected with the normal saline intraperitoneally single daily dose for 30 days. Group B injected intrapertioneally with cadmium chloride single daily dose 3.5 mg/kg body weight for 30 days. Group C injected intraperetonially with cadmium chloride in a dose of 3.5 mg/kg body weight. Intragastric tube was put to recieve parsley oil in a dose of 0.5 ml/kg body weight prior to cadmium injection. The two drugs were given for 30 days. Group D recieved 0.5 ml/kg body weight by intragastric tube of parsley oil for 30 days. At the end of this experiement, the animals were sacrified the lungs were collected from all groups and prepared for light microscopical examination. Histological changes were detected in cadmium chloride treated group in comparison with the control group including congestion, inflammatory cell infiltration, interstial pneumonia (decreased alveolar space), thickening of interalveolar septum and damge to the alveolar cells. All these changes were eliminated by giving parsley oil.

Download Full-text

Stress effects on food handler attention in a public hospital in Recife-PE, Brazil

Dementia & Neuropsychologia ◽

10.1590/s1980-57642010dn40400012 ◽

2010 ◽

Vol 4 (4) ◽

pp. 325-331 ◽

Cited By ~ 2

Author(s):

Renata de Melo Guerra Ribas ◽

Valdenilson Ribeiro Ribas ◽

Hugo André de Lima Martins ◽

Valéria Ribeiro Ribas ◽

Severino Marcos de Oliveira Carneiro ◽

...

Keyword(s):

Psychological Symptoms ◽

Digit Span ◽

Physical Symptoms ◽

Food Handler ◽

Control Group ◽

Negative Effects ◽

Chi Square ◽

Stress Effects ◽

Human Labor ◽

Serial Addition

Abstract Production of meals in Food and Nutrition Units (FNU) requires human labor performance in order to attend demand. Stress is one of the factors responsible for alterations in the health of these professionals. Reduced attention is one of the negative effects caused by stress. Objective: The objective of this study was to evaluate attention in Food Handlers (FH). Methods: 67 professionals were evaluated, subdivided into FH with less than 5 years in the profession, who were included as the control group (Control, n=29), and FH with more than 5 years in the profession (FH>5, n=38). Lipp's Inventory of Stress Symptoms for adults (LISS), the digit symbol, d2, forward digit span, backward digit span and paced auditory serial addition tests were applied. The data found on the LISS were analysed by Fisher's Exact and Chi-Square tests and individual attention tests were analyzed by the Mann-Whitney test, with data expressed as Median (Minimum and Maximum) with p<0.05. A total of 73.68% of FH>5 presented stress versus 13.79% of Control. Also, 57.89% of FH>5 with stress presented physical symptoms and 15.79% psychological symptoms. In the Control, 9.68% presented physical symptoms. Results: 73.68% of FH>5 with stress and 17.24% of the Control were in the resistance phase. Regarding attention, the FH>5 presented reduced focused attention (32.5±2.9), auditory attention (7.25±0.4) and resistance to interference (4.67±1.1), compared to their respective controls (43.88±1.39), (8.63±0.38), (9.25±1.16), p<0.05. Discussion and Conclusions: The activity of food handling can cause stress and attention level reduction after 5 years.

Download Full-text

Histological Effect of Aspirin on the Stomach of Male Albino Swiss Mice (Mus musculus)

Iraqi Journal of Science ◽

10.24996/ijs.2020.61.6.4 ◽

2020 ◽

pp. 1275-1281

Author(s):

Zainab Karim Al-Timimi

Keyword(s):

Body Weight ◽

Control Group ◽

Male Mice ◽

Histopathological Changes ◽

Negative Effects ◽

Successive Treatment ◽

Once Daily ◽

Muscular Layer ◽

Swiss Mice ◽

Histological Effect

The present study aimed to determine the impacts of Aspirin drug on the stomach of albino Swiss mice. The study sample included 10 male mice divided into 2 groups. The first group was orally administered with 0.1 mL of 0.75 mg/kg aspirin once daily, whereas the second group, the control, was treated with similar doses of distilled water. Following 60 days of successive treatment, a number of parameters was studied including difference in body weight and histopathological changes in the stomach as diagnosed after histological preparation. The results showed a significant decrease (p<0.05) in body weight average of the treated mice compared with the control group. The results also revealed the occurrence of several histopathological changes in the stomach of treated animals, including raptures in the epithelium lining, congestion in the muscular layer, and vasodilation. In addition, alterations such as hemorrhage, pyknosis in muscle cells, sloughing of the lining of the muscular layer, and external bleeding were observed. It can be concluded from these results that orally administered aspirin exerts negative effects on the stomach in mice.

Download Full-text