scholarly journals Evaluation of Multiple Impacts of Furfural Acetone on Nematodes In Vitro and Control Efficiency against Root-Knot Nematodes in Pots and Fields

Antibiotics ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 605
Author(s):  
Wanli Cheng ◽  
Xue Yang ◽  
Li Zeng ◽  
Dian Huang ◽  
Minmin Cai ◽  
...  
Keyword(s):  
Field Experiments ◽  
Plant Root ◽  
Reduction Rate ◽  
Disease Index ◽  
Economic Losses ◽  
Egg Hatching ◽  
Root Knot Nematodes ◽  
Control Efficiency ◽  
The Impact

Root-knot nematodes (RKNs) seriously endanger agricultural development and cause great economic losses worldwide. Natural product furfural acetone (FAc) is a promising nematicide with strong attractant and nematicidal activities, but baseline information about the impact of FAc on the reproduction, egg hatching, feeding, and growth of nematodes and its pest control efficiency in field are lacking. Here, the inhibition effects of FAc on nematodes in vitro and its RKN control efficiency in pot and field were investigated. FAc inhibited the egg hatching of Meloidogyne incognita by 91.7% at 200 mg/L after 2 days and suppressed the reproduction, feeding, and growth of Caenorhabditis elegans in vitro. In pot experiments, FAc in various dosages reduced the disease index of plant root significantly. In field experiments, FAc exhibited control effect on RKNs equivalent to commercial nematicides avermectin and metam sodium, with a reduction in disease index by 36.9% at a dose of 50 mg/plant. FAc also reduced the population density of RKNs in soil, with a reduction rate of 75.3% at the dose of 750 mg/m2. No adverse effect was detected on plant growth after FAc application. These results provide compelling evidence for development of FAc as an appropriate alternative for current nematicides.

scholarly journals Alternative Control of Phragmidium rubi-idaei Infecting Two Rubus Species

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1452
Author(s):  
Raluca-Maria Pârlici ◽  
Aurel Maxim ◽  
Stefania Mirela Mang ◽  
Ippolito Camele ◽  
Lucia Mihalescu ◽  
...  
Keyword(s):  
Organic Farming ◽  
Plant Pathogens ◽  
Field Experiments ◽  
Control Sample ◽  
Field Tests ◽  
In Vitro Tests ◽  
Rust Disease ◽  
Conidia Germination ◽  
The Impact

Organic berry plantations have been gaining popularity among farmers during recent years. Even so, farmers experience serious challenges in disease control management, which is a concern in organic farming. Phragmidiumrubi-idaei (DC) P. Karst is the pathogen responsible for blackberry and raspberry rust disease, one of the most present and active diseases in plantations. The antifungal certified products found on the organic farming market offer the opportunity for an efficient control strategy over plant pathogens in fruit shrub plantations. In this study, 5 natural based products—namely Altosan, Mimox, Canelys, Zitron, and Zeolite—were tested for their fungistatic effect over P. rubi-idaei. The experiments were carried out under laboratory conditions, performing observations over the impact of organic products, used at different concentration levels, on rust conidia germination. Moreover, field experiments were conducted in order to evaluate the efficiency of different treatments for rust control on raspberry (‘Polka’, ‘Veten’ and ‘Heritage’) and blackberry (‘Thorn Free’, ‘Chester’ and ‘Loch Ness’) varieties. Data analysis based on ANOVA tests showed significant differences between the tested variants and the control sample at p < 0.001. Furthermore, LSD test confirmed differences between all substances tested (p < 0.005). The natural products Canelys (formulated with cinnamon) and Zytron (based on citrus extract) have proven the highest inhibitory capacity for conidia germination during in vitro tests registering values of 80.42% and 78.34%, respectively. The same high inhibitory rates against rust pathogen were kept also in the field tests using the same two natural-based products mentioned earlier. In addition, outcomes from this study demonstrated that Zeolite is not recommended for raspberry or blackberry rust control.

scholarly journals Pathogenicity and Volatile Nematicidal Metabolites from Duddingtonia flagrans against Meloidogyne incognita

2021 ◽  
Vol 9 (11) ◽  
pp. 2268
Author(s):  
Xiaoyu Mei ◽  
Xin Wang ◽  
Guohong Li
Keyword(s):  
Meloidogyne Incognita ◽  
Plant Pathogens ◽  
Plant Root ◽  
Nematicidal Activity ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Egg Mass ◽  
Duddingtonia Flagrans ◽  
Egg Hatching ◽  
Root Knot Nematodes

Plant parasitic nematodes, especially parasitic root-knot nematodes, are one of the most destructive plant pathogens worldwide. The control of plant root-knot nematodes is extremely challenging. Duddingtonia flagrans is a type of nematode-trapping fungi (NTF), which produces three-dimensional adhesive networks to trap nematodes. In this study, the pathogenicity and volatile organic compounds (VOCs) of the NTF D. flagrans against the plant root-knot nematode, Meloidogyne incognita, were investigated. The predatory process of D. flagrans trapping M. incognita was observed using scanning electron microscopy. Gas chromatography-mass spectrometry analysis of the VOCs from D. flagrans led to the identification of 52 metabolites, of which 11 main compounds were tested individually for their activity against M. incognita. Three compounds, cyclohexanamine, cyclohexanone, and cyclohexanol, were toxic to M. incognita. Furthermore, these three VOCs inhibited egg hatching of M. incognita. Cyclohexanamine showed the highest nematicidal activity, which can cause 97.93% mortality of M. incognita at 8.71 µM within 12 h. The number of hatched juveniles per egg mass after 3 days was just 8.44 when treated with 26.14 µM cyclohexanamine. This study is the first to demonstrate the nematicidal activity of VOCs produced by D. flagrans against M. incognita, which indicates that D. flagrans has the potential to biocontrol plant root-knot nematodes.

scholarly journals In vitro and in vivo anthelmintic activity of extracts from Artemisia parviflora and A. sieversiana

2017 ◽  
Vol 54 (3) ◽  
pp. 218-224 ◽  
Author(s):  
S. Irum ◽  
H. Ahmed ◽  
B. Mirza ◽  
K. Donskow-Łysoniewska ◽  
A. Muhammad ◽  
...  
Keyword(s):  
Adult Worm ◽  
Plant Extracts ◽  
Small Ruminants ◽  
Anthelmintic Activity ◽  
Gastrointestinal Nematodes ◽  
Economic Losses ◽  
Egg Hatching ◽  
Infective Larvae

SummaryIn the northern areas of Pakistan, the use of Artemisia based therapeutics is a common practice. Plants of genus Artemisia are known to possess anthelmintic and therapeutic effect. Infections caused by gastrointestinal nematodes are major threat to livestock industry across the world resulting in loss of production and indirect economic losses due to high cost of anthelmintic drugs. Present study was carried out to evaluate in vitro and in vivo effect of Artemisia sieversiana and Artemisia parviflora on Haemonchus contortus, a parasitic nematode of small ruminants. Methanolic plant extract was tested against three different developmental stages using an egg hatch assay, infective larvae and adult worm motility assay. Different concentrations were used for the bioassays and post exposure mortality was recorded after 8 hr for adult worms and infective larvae, while egg inhibition percentage was observed after 27 hr. A highly significant ability to inhibit the egg hatching (100 %) was recorded for both plant extracts while, the highest activity for adult worm assay and larvicidal assay was 90 % for A. sieversiana. The highest activity for adult motility and larvicidal assay for A. parviflora was 89 % and 86.6 % respectively. For in vivo trials maximum parentage reduction was 77.0 % for A. sieversiana and 73.6 % for A. parviflora. It is concluded that selected plant extracts were effective in reducing worm burden in animals.

scholarly journals Evaluation of Preventive Fungicide Applications for Fairy Ring Control in Golf Putting Greens and In Vitro Sensitivity of Fairy Ring Species to Fungicides

Plant Disease ◽  
2012 ◽  
Vol 96 (7) ◽  
pp. 1001-1007 ◽  
Author(s):  
G. L. Miller ◽  
M. D. Soika ◽  
L. P. Tredway
Keyword(s):  
Soil Temperature ◽  
Field Experiments ◽  
Chemical Properties ◽  
Creeping Bentgrass ◽  
Fungicide Sensitivity ◽  
Putting Greens ◽  
Growth Assay ◽  
Ring Species ◽  
The Impact

Fairy ring species induce symptoms on putting greens mostly indirectly, by modifying the soil physical or chemical properties. Therefore, preventive rather than curative fungicide applications may be more effective in managing fairy ring. Two field experiments were conducted on a creeping bentgrass research green to evaluate fairy ring control from preventive fungicide applications. A 3-year study investigated the optimal rate and soil temperature-based timing of a preventive application of triadimefon and tebuconazole. A 2-year study evaluated the impact of irrigation timing and fungicide + surfactant tank mixtures on the efficacy of preventive applications of triadimefon and triticonazole. Fungicide-treated plots in both studies exhibited less fairy ring severity than untreated plots. Data suggest that a 5-day average soil temperature range of 13 to 16°C may be suitable for initiating preventive applications. Symptoms occurred earlier in plots treated with a surfactant tank mix than in those treated with fungicide alone. Irrigation timing had no effect on fungicide performance. The sensitivity of 16 isolates representing major fairy ring species to flutolanil, propiconazole, tebuconazole, triadimefon, and triticonazole was determined with a mycelial growth assay. No significant differences in fungicide sensitivity were detected among species. Isolates had significantly higher 50% effective concentration values for triadimefon than for the other fungicides tested.

scholarly journals HoBi-Like Pestivirus and Reproductive Disorders

2020 ◽  
Vol 7 ◽  
Author(s):  
Nicola Decaro
Keyword(s):  
Reproductive Tract ◽  
Diagnostic Methods ◽  
Economic Losses ◽  
Reproductive Disorders ◽  
Fatal Disease ◽  
Viral Diarrhea ◽  
Asymptomatic Infections ◽  
Cattle Reproduction ◽  
The Impact

HoBi-like pestivirus (HoBiPeV) is an emerging group of pestiviruses that has been detected in cattle and other ruminants in South America, Europe, and Asia. Analogous to other bovine pestiviruses, namely bovine viral diarrhea (BVDV) 1 and 2, HoBiPeV is able to cause a variety of clinical forms that range from asymptomatic infections to fatal disease, having a great impact on cattle productions and causing substantial economic losses, mainly as a consequence of the occurrence of reproductive failures. The manuscript aims to provide an updated review of the currently available literature about the impact of HoBiPeV infection on cattle reproduction. The reproductive disorders observed in cattle due to natural and experimental infections caused by this virus are reported along with the few available in-vitro studies involving the reproductive tract. HoBiPeV should be considered among the bovine pathogens that impact on reproduction, but there is a need for more specific and sensitive diagnostic methods, while the cross-protection elicited by commercially available BVDV vaccines should be better investigated.

scholarly journals Impact of Trichoderma sp. on the Development of Heterobasidion Annosum in Decayed Understory Picea Abies Stumps

2017 ◽  
Vol 71 (1-2) ◽  
pp. 52-56
Author(s):  
Kristīne Kenigsvalde ◽  
Dina Nitiša ◽  
Dace Saulīte ◽  
Kari Korhonen ◽  
Līvija Vulfa ◽  
...  
Keyword(s):  
Biological Control ◽  
Field Studies ◽  
Antagonistic Effect ◽  
Heterobasidion Annosum ◽  
Economic Losses ◽  
Biological Control Agents ◽  
Trichoderma Sp ◽  
The Impact ◽  
Trichoderma Isolates

Abstract Heterobasidion annosum (Fr.) Bref. s.l. causes significant economic losses in conifer forests. Therefore, to reduce the spread of the infection surfaces of freshly cut conifer, stumps are commonly treated with biological control agents. Trichoderma sp. shows very strong antagonistic effect against H. annosum in vitro, but relatively few field studies have been conducted. Moreover, most of previous studies examined the impact of Trichoderma sp. on H. annosum in healthy conifer stumps. The aim of our work was to evaluate the effect of Trichoderma sp. against H. annosum in already decayed understory spruce stumps. In total, 75 decayed spruce stumps were surveyed. Part of the spruce stumps were left as a control, and the others were treated with one of two Trichoderma isolates (T472 and T945) belonging to two different species: T. viridescens and T. viride. The occurrence of H. annosum was evaluated 3 and 12 months after treatment. The main results were that the area of previously healthy wood occupied by H. annosum was larger in control stumps in comparison with treated stumps, but the differences were not statistically significant.

Biocontrol efficacy of Bacillus cereus strain Bc-cm103 against Meloidogyne incognita

Plant Disease ◽  
2021 ◽  
Author(s):  
Nan Yin ◽  
JianLong zhao ◽  
Rui Liu ◽  
Yan Li ◽  
Jian Ling ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Meloidogyne Incognita ◽  
Laser Scanning ◽  
Fermentation Broth ◽  
Laser Scanning Microscopy ◽  
Economic Losses ◽  
Confocal Laser ◽  
Hatching Rate ◽  
Egg Hatching ◽  
Root Knot Nematodes

Root-knot nematodes (Meloidogyne spp.) are soil-borne pathogens that infect vegetable crops and cause major economic losses worldwide annually. Therefore, there is an urgent need for novel nematicides or biological control agents to reduce the damage caused by root-knot nematodes. In this study, we tested efficacy of the Bacillus cereus strain Bc-cm103, isolated from the rhizoplane of Cucumis metuliferus, against Meloidogyne incognita. The strain Bc-cm103 fermentation broth caused 100% mortality of the nematode second-stage juveniles (J2s) within 12 h and decreased the egg hatching rate by 40.06% within 72 h compared with sterile water. Confocal laser scanning microscopy revealed that strain Bc-cm103 formed a biofilm on cucumber (Cucumis sativus) roots, which protected the roots from the infection of M. incognita. Additionally, strain Bc-cm103 activated the defense-responsive genes PR1, PR2, LOX1, and CTR1 in cucumber. Furthermore, strain Bc-cm103 significantly reduced the appearance of root galls in pot, split-root and field tests. These results indicated that the B. cereus strain Bc-cm103 had a strong suppressive effect on M. incognita and therefore could be used as a potential biocontrol agent against this pathogen.

scholarly journals Wasted Children and Wasted Time: A Challenge to Meeting the Nutrition Sustainable Development Goals with a High Economic Impact to Ethiopia

Nutrients ◽  
2020 ◽  
Vol 12 (12) ◽  
pp. 3698
Author(s):  
Arnaud Laillou ◽  
Kaleab Baye ◽  
Zelalem Meseret ◽  
Hiwot Darsene ◽  
Abdulai Rashid ◽  
...  
Keyword(s):  
Sustainable Development ◽  
Reduction Rate ◽  
Therapeutic Interventions ◽  
Economic Losses ◽  
The Sustainable Development ◽  
Weather Shocks ◽  
Prevention Interventions ◽  
Slow Progress ◽  
Development Goals ◽  
The Impact

Despite some progress in the reduction of the prevalence of child wasting in Ethiopia, the pace of progress has been slow. Despite millions of dollars being spent on the treatment of wasting every year, the increased frequency and magnitude of environmental and anthropogenic shocks has halted progress. This study aimed to present the trends of child wasting in Ethiopia and estimate the economic losses related to the slow progress towards meeting the sustainable development goal (SDG) targets. Weather shocks and civil unrest between 2015 and 2018 have halted progress. We used a “consequence model” to apply the coefficient risk–deficit on economic losses established in the global scientific literature to the Ethiopian health, demographic, and economic data to estimate economic losses related to child wasting. The impact of wasting on the national economy of Ethiopia is estimated to be 157.8–230.2 million United States dollars (USD), annually. The greatest contributor to the economic burden (43.5–63.5% of the burden depending on the discount rate) is the cost of supplies and human resources to treat wasting. To reach the 2030 SDGs, Ethiopia should increase its annual average reduction rate (AARR) in the numbers of child (<59 months) wasting from 0.1% to 5.4%. This will avert the wasting in 7.9 million cases and prevent additional economic costs of up to 803.7 million USD over the next decade. Increasing the reach of therapeutic interventions, but also identifying and implementing wasting prevention interventions, will be critical if the SDG targets are to be met and the opportunity of the children to thrive is not to be wasted.

scholarly journals Weaning-associated feed deprivation stress causes microbiota disruptions in a novel mucin-containing in vitro model of the piglet colon (MPigut-IVM)

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Raphaële Gresse ◽  
Frédérique Chaucheyras-Durand ◽  
Sylvain Denis ◽  
Martin Beaumont ◽  
Tom Van de Wiele ◽  
...  
Keyword(s):  
Animal Experiments ◽  
Taxonomic Composition ◽  
Economic Losses ◽  
Strong Increase ◽  
Swine Industry ◽  
Feed Deprivation ◽  
And Function ◽  
The Impact

Abstract Background Risk factors for the etiology of post-weaning diarrhea, a major problem in swine industry associated with enormous economic losses, remain to be fully elucidated. In concordance with the ethical concerns raised by animal experiments, we developed a new in vitro model of the weaning piglet colon (MPigut-IVM) including a mucin bead compartment to reproduce the mucus surface from the gut to which gut microbes can adhere. Results Our results indicated that the MPigut-IVM is able to establish a representative piglet archaeal and bacterial colon microbiota in terms of taxonomic composition and function. The MPigut-IVM was consequently used to investigate the potential effects of feed deprivation, a common consequence of weaning in piglets, on the microbiota. The lack of nutrients in the MPigut-IVM led to an increased abundance of Prevotellaceae and Escherichia-Shigella and a decrease in Bacteroidiaceae and confirms previous in vivo findings. On top of a strong increase in redox potential, the feed deprivation stress induced modifications of microbial metabolite production such as a decrease in acetate and an increase in proportional valerate, isovalerate and isobutyrate production. Conclusions The MPigut-IVM is able to simulate luminal and mucosal piglet microbiota and represent an innovative tool for comparative studies to investigate the impact of weaning stressors on piglet microbiota. Besides, weaning-associated feed deprivation in piglets provokes disruptions of MPigut-IVM microbiota composition and functionality and could be implicated in the onset of post-weaning dysbiosis in piglets.

scholarly journals In vitro evaluation of some isolated bacteria against the plant parasite nematode Meloidogyne incognita

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Gaziea Mohamed Soliman ◽  
Hoda Hussein Ameen ◽  
Shadia Mohamed Abdel-Aziz ◽  
Ghada M. El-Sayed
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Bacillus Subtilis ◽  
Bacillus Cereus ◽  
Meloidogyne Incognita ◽  
Paenibacillus Polymyxa ◽  
Achromobacter Xylosoxidans ◽  
Egg Hatching ◽  
Root Knot Nematodes ◽  
Lysinibacillus Sphaericus

Abstract Background Microbial cultures are extensively used as environment friendly ways for biological control of parasitic pests, including the root-knot nematodes, alternative to the use of chemical nematicides. The present study was conducted to isolate some lytic rhizobacteria and examine their nematicidal activity against Meloidogyne incognita J2 mortality and egg hatching in in vitro test. Results Lytic Rhizobacteria were isolated from soil samples adhering to tomato plant roots from different localities at Giza governorate, Egypt. Six bacterial isolates, exhibited high efficacy against root-knot nematodes, were identified based on the analysis of the 16S rRNA gene sequence as Pseudomonas aeruginosa, Paenibacillus polymyxa, Lysinibacillus sphaericus, Bacillus cereus, Bacillus subtilis, and Achromobacter xylosoxidans. These strains showed high production of chitinase, chitosanase, and protease using colloidal chitin and soluble chitosan as carbon sources. Two strains, Paenibacillus polymyxa and Bacillus subtilis, produced the highest levels of chitinase in the media. Except for Pseudomonas aeruginosa, all the bacterial strains produced high levels of chitosanase. Conclusion The results revealed that high amounts of protease were, however, secreted by Pseudomonas aeruginosa as compared to the other strains. In in vitro tests, all the bacterial culture filtrates potentially displayed nematicidal effect in M. incognita egg hatching and an obvious increase in J2 mortality as compared to control. Paenibacillus polymyxa caused 100% juvenile mortality followed by Bacillus subtilis 97.25%, Bacillus cereus 94%, Achromobacter xylosoxidans 93%, Lysinibacillus sphaericus 92%, and Pseudomonas aeruginosa 84.29% after 48 h of exposure, as compared to control.

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