scholarly journals Assessment of Brain Functional Activity Using a Miniaturized Head-Mounted Scanning Photoacoustic Imaging System in Awake and Freely Moving Rats

Biosensors ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 429
Author(s):  
Yuhling Wang ◽  
Tsung-Sheng Chu ◽  
Yan-Ren Lin ◽  
Chia-Hui Tsao ◽  
Chia-Hua Tsai ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Photoacoustic Imaging ◽  
T Test ◽  
Natural Behavior ◽  
Freely Moving Rats ◽  
Anesthetized Rats ◽  
Freely Moving ◽  
Paired T Test ◽  
Awake Rats

Understanding the relationship between brain function and natural behavior remains a significant challenge in neuroscience because there are very few convincing imaging/recording tools available for the evaluation of awake and freely moving animals. Here, we employed a miniaturized head-mounted scanning photoacoustic imaging (hmPAI) system to image real-time cortical dynamics. A compact photoacoustic (PA) probe based on four in-house optical fiber pads and a single custom-made 48-MHz focused ultrasound transducer was designed to enable focused dark-field PA imaging, and miniature linear motors were included to enable two-dimensional (2D) scanning. The total dimensions and weight of the proposed hmPAI system are only approximately 50 × 64 × 48 mm and 58.7 g (excluding cables). Our ex vivo phantom experimental tests revealed that a spatial resolution of approximately 0.225 mm could be achieved at a depth of 9 mm. Our in vivo results further revealed that the diameters of cortical vessels draining into the superior sagittal sinus (SSS) could be clearly imaged and continuously observed in both anesthetized rats and awake, freely moving rats. Statistical analysis showed that the full width at half maximum (FWHM) of the PA A-line signals (relative to the blood vessel diameter) was significantly increased in the selected SSS-drained cortical vessels of awake rats (0.58 ± 0.17 mm) compared with those of anesthetized rats (0.31 ± 0.09 mm) (p < 0.01, paired t-test). In addition, the number of pixels in PA B-scan images (relative to the cerebral blood volume (CBV)) was also significantly increased in the selected SSS-drained blood vessels of awake rats (107.66 ± 23.02 pixels) compared with those of anesthetized rats (81.99 ± 21.52 pixels) (p < 0.01, paired t-test). This outcome may result from a more active brain in awake rats than in anesthetized rats, which caused cerebral blood vessels to transport more blood to meet the increased nutrient demand of the tissue, resulting in an obvious increase in blood vessel volume. This hmPAI system was further validated for utility in the brains of awake and freely moving rats, showing that their natural behavior was unimpaired during vascular imaging, thereby providing novel opportunities for studies of behavior, cognition, and preclinical models of brain diseases.

Respiratory sinus arrhythmia in freely moving and anesthetized rats

2004 ◽  
Vol 97 (4) ◽  
pp. 1431-1436 ◽  
Author(s):  
Evguenia Bouairi ◽  
Robert Neff ◽  
Cory Evans ◽  
Allison Gold ◽  
Michael C. Andresen ◽  
...  
Keyword(s):  
Heart Rate ◽  
Respiratory Sinus Arrhythmia ◽  
Vagal Activity ◽  
General Anesthetics ◽  
Sinus Arrhythmia ◽  
Lung Inflation ◽  
Pentobarbital Sodium ◽  
Freely Moving Rats ◽  
Anesthetized Rats ◽  
Freely Moving

Heart rate increases during inspiration and slows during postinspiration; this respiratory sinus arrhythmia helps match pulmonary blood flow to lung inflation and maintain an appropriate diffusion gradient of oxygen in the lungs. This cardiorespiratory pattern is found in neonatal and adult humans, baboons, dogs, rabbits, and seals. Respiratory sinus arrhythmia occurs mainly due to inhibition of cardioinhibitory parasympathetic cardiac vagal neurons during inspiration. Surprisingly, however, a recent study in anesthetized rats paradoxically found an enhancement of cardiac vagal activity during inspiration, suggesting that rats have an inverted respiratory sinus arrhythmia (Rentero N, Cividjian A, Trevaks D, Pequignot JM, Quintin L, and McAllen RM. Am J Physiol Regul Integr Comp Physiol 283: R1327–R1334, 2002). To address this controversy, this study examined respiratory sinus arrhythmia in conscious freely moving rats and tested whether the commonly used experimental anesthetics urethane, pentobarbital sodium, or ketamine-xylazine alter respiratory sinus arrhythmia. Heart rate significantly increased 21 beats/min during inspiration in conscious rats, a pattern similar to the respiratory sinus arrhythmia that occurs in other species. However, anesthetics altered normal respiratory sinus arrhythmia. Ketamine-xylazine (87 mg/kg and 13 mg/kg) depressed and pentobarbital sodium (60 mg/kg) abolished normal respiratory sinus arrhythmia. Urethane (1 g/kg) inverted the cardiorespiratory pattern so that heart rate significantly decreased during inspiration. Our study demonstrates that heart rate normally increases during inspiration in conscious, freely moving rats, similar to the respiratory sinus arrhythmia pattern that occurs in other species but that this pattern is disrupted in the presence of general anesthetics, including inversion in the case of urethane. The presence and consequences of anesthetics need to be considered in studying the parasympathetic control of heart rate.

COMPARISON OF ANTIHYPERTENSIVE EFFECT OF MOXONIDINE VERSUS CLONIDINE IN RENAL FAILURE PATIENTS.

2018 ◽  
Vol 6 (9) ◽  
Author(s):  
DR.MATHEW GEORGE ◽  
DR.LINCY JOSEPH ◽  
MRS.DEEPTHI MATHEW ◽  
ALISHA MARIA SHAJI ◽  
BIJI JOSEPH ◽  
...  
Keyword(s):  
Blood Pressure ◽  
Renal Failure ◽  
Blood Flow ◽  
Blood Vessel ◽  
Blood Vessels ◽  
High Blood Pressure ◽  
Antihypertensive Effect ◽  
The Body ◽  
Ability To Work ◽  
Blood Flows

Blood pressure is the force of blood pushing against blood vessel walls as the heart pumps out blood, and high blood pressure, also called hypertension, is an increase in the amount of force that blood places on blood vessels as it moves through the body. Factors that can increase this force include higher blood volume due to extra fluid in the blood and blood vessels that are narrow, stiff, or clogged(1). High blood pressure can damage blood vessels in the kidneys, reducing their ability to work properly. When the force of blood flow is high, blood vessels stretch so blood flows more easily. Eventually, this stretching scars and weakens blood vessels throughout the body, including those in the kidneys.

Efektivitas Aroma Terapi Lemon untuk Menangani Emesis Gravidarum

2019 ◽  
Vol 11 (4) ◽  
pp. 277-284
Author(s):  
Vitrianingsih Vitrianingsih ◽  
Sitti Khadijah
Keyword(s):  
Pregnant Women ◽  
Sampling Technique ◽  
T Test ◽  
The Body ◽  
Nausea And Vomiting ◽  
P Value ◽  
Purposive Sampling ◽  
Body Tissues ◽  
Quasi Experimental ◽  
Paired T Test

Studi memperkirakan emesis gravidarum terjadi pada 50-90% kehamilan. Mual muntah pada kehamilan memberikan dampak yang signifikan bagi tubuh dimana ibu menjadi lemah, pucat dan cairan tubuh berkurang sehingga darah menjadi kental (hemokonsentrasi). Keadaan ini dapat memperlambat peredaran darah dan berakibat pada kurangnya suplay oksigen serta makanan ke jaringan sehingga dapat membahayakan kesehatan ibu dan janin. Salah satu terapi yang aman dan dapat dilakukan untuk mengurangi keluahan mual muntah pada ibu hamil adalah pemberian aromaterapi lemon. Penelitian bertujuan untuk mengetahui efektifitas aroma terapi lemon untuk menangani emesis gravidarum. Penelitian ini menggunakan rancangan Quasi experiment  dengan  one group pre-post test design. Populasi penelitian adalah ibu hamil yang mengalami emesis gravidarum di Kecamatan Berbah, Sleman. Jumlah sampel 20 ibu hamil trimester pertama yang diambil dengan teknik purposive sampling. Pengukuran mual muntah dilakukan debelum dan setelah  pemberian aromaterapi lemon menggunakan Indeks Rhodes. Analisa data menggunakan uji Paired t-test. Hasil penelitian didapatkan rata-rata skor mual muntah sebelum pemberian aromaterapi lemon berdasarkan Indeks Rhodes pada Ibu Hamil dengan emesis gravidarum yaitu 22,1 dan terjadi penurunan skor setelah pemberian aromaterapi lemon menjadi 19,8. Ada pengaruh pemberian aromaterapi lemon dengan pengurangan mual muntah pada ibu hamil (p-value = 0.017). Berdasarkan hasil penelitian dapat disimpulkan pemberian aromaterapi lemon efektif untuk mengurangi emesis gravidarum pada ibu hamil trimester pertama.  Kata kunci: aromaterapi lemon, emesis gravidarum THE EFFECTIVENESS OF LEMON AROMATHERAPY FOR HANDLING EMESIS GRAVIDARUM   ABSTRACT Studies estimate that nausea and vomiting (emesis gravidarum) occur in 50 – 90% of pregnancies. Nausea and vomiting of pregnancy have a significant impact on the body in which it makes a mother becomes weak, pale, and decreasing body fluid so that the blood becomes thick (hemoconcentration). This situation can slow down blood circulation and inflict the lack of oxygen and food supplies to the body tissues so that it can endanger the health of the mother and fetus. One of the therapies that is safe and can be conducted to reduce nausea and vomiting of pregnancy is by giving the lemon aromatherapy treatment. The research aims to determine the effectiveness of the aroma of lemon therapy to deal with emesis gravidarum. This study applied quasi-experimental research with one group pretest-posttest design. The population of this study was pregnant women who experienced emesis gravidarum. Furthermore, samples were 20 mothers from Berbah, Sleman taken by using a purposive sampling technique. Nausea and vomiting were assessed between before and after giving lemon aromatherapy using the Rhodes Index. The data were analyzed using the paired t-test. The mean score of nausea and vomiting before giving lemon aromatherapy on mother with emesis gravidarum based on the Rhodes Index was 22.1. However, it decreased after given lemon aromatherapy treatment to 19.8. Therefore, there was an effect on giving lemon aromatherapy treatment toward the decrease of nausea and vomiting for pregnant women (p-value = 0.017). Lemon aromatherapy is effective to reduce emesis gravidarum.  Keywords: lemon aromatherapy, emesis gravidarum

PENGARUH PRACTICE BASED SIMULATION MODEL TERHADAP CRITICAL THINKING DI NURSING SKILL LABORATORY

2018 ◽  
Vol 4 (1) ◽  
Author(s):  
Retno Issroviatiningrum ◽  
Shanti Wardaningsih ◽  
Novita Kurnia Sari
Keyword(s):  
Critical Thinking ◽  
Simulation Model ◽  
Random Sampling ◽  
Simple Random Sampling ◽  
T Test ◽  
Control Group ◽  
Control Group Design ◽  
Group Design ◽  
Skill Laboratory ◽  
Paired T Test

ABSTRAK Upaya untuk meningkatkan mutu pendidikan keperawatan khususnya untuk mendukung peserta didik menjadi perawat profesional memerlukan proses pembelajaran dengan menggunakan fasilitas keterampilan klinis. Practice based simulation model didasarkan pada teori belajar konstruktif yang menegaskan bahwa pengetahuan tidak pasif ditransfer dari pendidik kepada peserta didik, tetapi dibangun oleh peserta didik melalui pengolahan pengalaman dan interaksi dengan lingkungan mereka. Dengan metode simulasi di laboratorium dapat mendorong mahasiswa untuk menggunakan critical thinking dalam mengambil keputusan dalam mengatasi masalah tanpa merugikan pasien yang sebenarnya. Penelitian ini bertujuan untuk menganalisis pengaruh practice based simulation model terhadap critical thinking pada mahasiswa semester VI di FIK Unissula Semarang. Penelitian ini menggunakan metode Quasy – Experiment dengan pendekatan pretest-posttest with control group design. Pengambilan sampel dengan teknik  simple random sampling dengan jumlah 21 responden baik kelompok intervensi maupun kelompok kontrol. Analisis data menggunakan uji Paired T-Test dan Independent Samples T-Test. Hasil penelitian selisih peningkatan critical thinking pada kelompok intervensi sebanyak 11.95 poin dan pada kelompok kontrol 2.05. Practice based simulation model berpengaruh terhadap critical thinking dengan nilai p=0.00<0.05. Disimpulkan bahwa practice based simulation model mempengaruhi critical thinking pada mahasiswa semester VI FIK Unissula Semarang. Kata kunci: Practice based simulation model, critical thinking

Pengaruh media teka-teki silang terhadap pengetahuan gizi seimbang pada anak sekolah dasar

2019 ◽  
Vol 2 (2) ◽  
pp. 107
Author(s):  
Umi Mahmudah
Keyword(s):  
T Test ◽  
Control Group ◽  
Paired T Test ◽  
Quasi Experiment

Latar Belakang: Anak-anak sekolah dasar merupakan salah satu kelompok rawan masalah gizi, baik pada gizi kurang maupun gizi lebih. Pendidikan gizi seimbang perlu diterapkan untuk mengatasi masalah gizi tersebut. Metode pendidikan mendorong peran serta dan keterlibatan anak untuk memberikan motivasi dalam belajar. Berbagai metode pendidikan yang menarik bagi anak antara lain permainan, tebak-tebakan, diskusi kelompok, serta peragaan. Tujuan: Untuk mengetahui pengaruh pendidikan gizi menggunakan media teka-teki silang (TTS) terhadap pengetahuan gizi seimbang pada anak sekolah dasar. Metode: Penelitian ini merupakan penelitian quasi experiment dengan rancangan pre-posttest with control group. Penelitian dilakukan pada anak Sekolah Dasar Negeri Donohudan I. Pengukuran pengetahuan gizi seimbang dilakukan sebanyak dua kali, yaitu pretest dan posttest. Penelitian ini dibagi menjadi dua kelompok, yaitu kelompok intervensi yang diberikan pendidikan gizi menggunakan teka-teki silang dan kelompok kontrol yang diberikan pendidikan gizi menggunakan ceramah. Pengukuran pengetahuan pretest dilakukan sebelum diberikan pendidikan gizi dan pengukuran pengetahuan posttest diberikan setelah dilakukan pendidikan gizi. Data dianalisis menggunakan Paired T-test dan Independent Sample T-test. Hasil: Terdapat perbedaan pengetahuan sebelum dan setelah dilakukan pendidikan gizi menggunakan media TTS dan media ceramah (p=0,010). Rerata peningkatan pengetahuan gizi menggunakan media TTS lebih tinggi dibandingkan dengan menggunakan metode ceramah pada anak sekolah dasar. Kesimpulan: Ada pengaruh pengetahuan gizi seimbang sebelum dan setelah diberikan pendidikan gizi, baik menggunakan media teka-teki silang maupun menggunakan media ceramah.

LIFE REVIEW THERAPY MENURUNKAN TINGKAT DEPRESI LANSIA PADA WARGA BINAAN SOSIAL DI PANTI SOSIAL TRESNA WERDHA BUDI MULIA 3 JAKARTA SELATAN

2020 ◽  
Vol 15 (2) ◽  
pp. 114-121
Author(s):  
Maryati . ◽  
Eka Rokhmiati Wahyu Purnamasari
Keyword(s):  
Control Design ◽  
Life Review ◽  
T Test ◽  
Post Test ◽  
Life Review Therapy ◽  
Paired T Test ◽  
Quasi Experiment

Life Review Therapy merupakanterapi psikoterapi untuk menyelesaikan masalah pada lansia yang mengalami depresi, mengekspresikan perasaan yang disupresikan sehingga energy psikis tersebut dilepaskan. Tujuan penelitian ini untuk mengetahui pengaruh Life Review Therapy terhadap tingkat depresi lansia pada warga binaan sosial di PSTW Budi Mulia Jakarta Selatan.Metode yang digunakan adalah quasi experiment, dengan pendekatan Pre Test and Post Test Without Control design. Populasi dalam penelitian ini adalah lansia dengan jumlah sampel 20 orang dengan teknik purposivesampling. Analisis data menggunakan Paired T Test dengan tingkat signifikan (α0,05). Hasil menunjukan bahwa ada pengaruh Life Review Therapy terhadap tingkat depresi lansiadengan ρValue=0,000. Berdasarkan penelitian tersebut, terapi Life Reviewterbukti dapat menurunkan tingkat depresi sehingga terapi ini dapat dijadikan salah satu acuan terapi untuk menangani depresi pada lansia di PSTW

PENGARUH HEALTH EDUCATION TERHADAP KEPATUHAN PERILAKU HIDUP BERSI SEHAT (PHBS) WARGA

2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Tatiana Siregar ◽  
Nelly Febriani
Keyword(s):  
Health Education ◽  
T Test ◽  
P Value ◽  
Purposive Sampling ◽  
Post Test ◽  
Paired T Test ◽  
Quasi Experiment

Latar belakang: Kondisi sasaran pencapaian Indonesia Sehat 2015 dari program MDG’s yang belum tercapai, sehingga dilanjutkan dengan program SDGs menjadikan Indonesia harus banyak berperan dalam semua kegiatan khsusnya di bidang kesehatan. Proses pencapaian cakupan program kesehatan sangat dipengaruhi oleh Health education yang dilakukan petugas kesehatan kepada warga, kesehatan masyarakat. Tujuan: Penelitain ini bertujuan untuk mengetahui perubahan perilaku hidup bersih sehat yang  dilaksanakan warga setelah diberikan intervesi health education.  Metode: Metode penelitian dilaksanakan secara quasi experiment pre dan post test.  Teknik mengambil sampel secara purposive Sampling pada 30 reponden kelompook intervensi dan 26 responden kelompok kontrol. Analisa data dilakukan secara paired t test. Hasil: Hasil di dapat ada perbedaan yang bermakna antara pengetahuan dan perubahan perilaku hidup bersih sehat repsonden sebelum dan sesudah diberi penyuluhan p value = 0,000. Saran: Diharapkan pemerintah setempat menggerakkan petugas kesehatan bersama-sama dengan warga melakukan perilaku hidup bersih sehat dalam kehidupan sehari-hari dengan memfasilitasi  sarana penunjang untuk menjalankan perilaku hidup bersih sehat, dengan maksimal pada warga. Kata kunci: Health eduation,  Perilaku Hidup Bersih Sehat

PENGARUH MEDIA GAMBAR TERHADAP KEMAMPUAN MENULIS PUISI SISWA

2018 ◽  
Vol 2 (1) ◽  
Author(s):  
Sinta Maria Dewi
Keyword(s):  
Design Method ◽  
Writing Skills ◽  
Class Action ◽  
T Test ◽  
Control Group ◽  
Control Group Design ◽  
Media Image ◽  
Group Design ◽  
Random Method ◽  
Paired T Test

Abstract: This research aims to know the Influence of Media Image on the ability of Writing Poetry students. The method used in this study is a random method that does not use posttes control group design method. The findings of this study show the average way of writing poetry on students by using the image media (experimental class) higher average poetry writing skills on students who were taught with conventional learning (control class). The average of pretest experiments obtained by experiment class is 63,75. The average pretest class of control class is 61,05. After the second class action, the average posttest of the experimental class is 79.45 and the control class is 74.95. Hypothesis calculation using t paired t test test and significance of 0.05 significant level indicates probability (significance) is 0,033. Because of the significance of 0.033

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