Evaluation of Antioxidant Activities, Oxidation Enzymes, and Quality of Nano-Coated Button Mushrooms (Agaricus Bisporus) during Storage

Agaricus Bisporus is an edible button mushroom that is highly perishable with an extremely short shelf-life at ambient temperature. This work aims to evaluate some antioxidant activities, oxidation enzymes, and determine postharvest qualities of nano-coated mushrooms with the combination of chitosan (CHN) component during storage at 4 °C for (0, 3, 6, 9, and 12 days). Silica/CHN Film strongly delayed the mushroom respiratory spike onset and blocked carbon dioxide passage from inside to the outside, while Titanium/CHN Film (0.035 mmol s−1 kg−1) established the lowest O2 production rate and thiobarbituric acid reactive substances production (6.21 nmol g−1). Silica/CHN Film was mainly effectual for the polyphenol contents (0.39 g kg−1) and antioxidant activities (78.14% and 71.09%) for DPPH and ABTS+ radical scavenging activities, respectively. The results reported that Silica/CHN Film induced the highest (catalase and ascorbate peroxidase) activities, while Titanium/CHN Film recorded the highest (peroxidase and superoxide dismutase) activities of antioxidant enzymes. Besides, Titanium/CHN Film preserved relatively lower contents of hydrogen peroxide (22.40 µmol g−1) and hydroxyl radical (0.16 µmol g−1). In a word, nano-materials used in coating films such as titanium or even silica with the combination of CHN can directly reduce the cell degradation, oxidation processes and enhance the harvested horticultural crops.

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Aplikasi aktivitas antioksidan tepung gathot (singkong terfermentasi) dalam edible film sosis ayam di suhu ruang

Livestock and Animal Research ◽

10.20961/lar.v19i2.51837 ◽

2021 ◽

Vol 19 (2) ◽

pp. 227

Author(s):

Astari Ratnaduhita ◽

Adi Magna Patriadi Nuhriawangsa ◽

Lilik Retna Kartikasari

Keyword(s):

Room Temperature ◽

Ph Value ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Thiobarbituric Acid ◽

Acid Value ◽

Edible Film ◽

Thiobarbituric Acid Value ◽

Materials Used

Objective: The objective of this research is to examine the antioxidant properties of gathot and its potential as edible film.Methods: The materials used were dry gathot, carrageenan, and glycerol. The method was conducted experimentally, antioxidant activities test of gathot flour were analyzed descriptively. Physicochemical test of the chicken sausage was analyzed quantitatively by RAL with a 2x5 factorial pattern. The first factor was the types of edible film (0.00 and 0.75%), and the second factor was storage time (0; 2; 4; 6; 8 days) with seven repetitions each variables. The quantitative data were processed by Minitab 19.0.Results: The results of the antioxidant test of gathot flour were antioxidant activities of 26.174 ppm; scavenging ability of 49.37%; phenol of 4,852.84µg/g; flavonoids of 4,520.30µg/g. The chicken sausage for eight days at room temperature has a decrease in pH value, a* and b*, and an increase in TBA (Thiobarbituric Acid) value and water content.Conclusions: The antioxidant level of gathot flour is classified as a weak antioxidant, the results in the application of gathot flour edible film with a concentration of 0.75% were able to maintain the quality of chicken sausage until day six at room temperature storage (28 ºC).

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Review on in vitro antioxidant activities of Curcuma species commonly used as herbal components in Indonesia

Food Research ◽

10.26656/fr.2017.4(2).163 ◽

2019 ◽

Vol 4 (2) ◽

pp. 286-293 ◽

Cited By ~ 1

Author(s):

Abdul Rohman ◽

H. Widodo ◽

E. Lukitaningsih ◽

M. Rafi ◽

Nurrulhidayah A.F. ◽

...

Keyword(s):

Antioxidant Activities ◽

Curcuma Longa ◽

Radical Scavenging ◽

Thiobarbituric Acid ◽

Natural Antioxidants ◽

In Vitro Tests ◽

Antioxidant Power ◽

Ferric Thiocyanate ◽

Herbal Components

Free radicals, reactive nitrogen species (RNS) and reactive oxygen species (ROS) have been known to contribute several degenerative diseases such as cardiovascular diseases, aging, certain types of cancers, rheumatoid arthritis, neurodegenerative, and diabetes mellitus. In order to overcome the negative effects of these radicals, some scientists have explored some natural antioxidants from plants and it's by-products. The antioxidant can be defined as any substances or samples capable of inhibiting free radical reactions in the oxidation reaction. Due to curcuminoids contained, Curcuma species such as Curcuma longa, Curcuma heyneana, Curcuma mangga, and Curcuma xanthorriza were commonly used for herbal components in some traditional medicine. Several in vitro tests been introduced and used to measure antioxidant activities, namely radical scavenging assay using 2,2’-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis-(3-ethylbenzothiazoline-6 -sulfonic acid) (ABTS•+), ferric reducing antioxidant power (FRAP), ferric-thiocyanate, phosphomolybdenum method, cupric ion reducing antioxidant capacity, metal chelating power, beta-carotene bleaching linoleic-ferric-thiocyanate, and thiobarbituric acid methods. This review highlighted the antioxidant activities in vitro of C. longa, C. heyneana, C. mangga, and C. xanthorriza through several tests. To perform this review, several repute databases were analyzed and used. From this review, it can be stated that Curcuma species have powerful antioxidant activities, therefore they could be potential sources of natural antioxidants and can be used as food supplements.

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Lipid Peroxidation and Antioxidant Activities of the Aqueous Rhizome Extract of Rheum officinale Baillon

Journal of Food Quality ◽

10.1155/2018/5258276 ◽

2018 ◽

Vol 2018 ◽

pp. 1-7 ◽

Cited By ~ 1

Author(s):

Eugene Chang ◽

Choon Young Kim

Keyword(s):

Lipid Oxidation ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Thiobarbituric Acid ◽

Natural Antioxidant ◽

Total Phenolic ◽

Ros Generation ◽

Thiobarbituric Acid Reactive Substance ◽

Superoxide Anion Production

Generation of reactive oxygen species (ROS) is associated with dysregulation of antioxidant defense mechanisms and incidence of human diseases. The specific aim of this study was to investigate the lipid oxidation and antioxidant activity of aqueous extract of Rheum officinale Baillon rhizome in order to evaluate its potential as a future novel natural antioxidant resource and a functional ingredient in food and pharmaceutical formations. Total phenolic and flavonoid contents of Rheum rhizome extract were dose dependently increased. Consistent with this, radical scavenging activities of Rheum rhizome extract as determined by 2,2-diphenyl-1-picrylhydrazyl assay and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) radical scavenging activity were significantly elevated as the concentration increased. In addition, the treatment of aqueous Rheum rhizome extract significantly increased ferric reducing and copper chelating activities. According to results of thiobarbituric acid reactive substance analysis, Rheum rhizome extract significantly delayed lipid oxidation. Preincubation with Rheum rhizome extract significantly inhibited tert-butyl hydroperoxide- (t-BHP-) induced ROS generation. Moreover, superoxide anion production was significantly lower in Rheum rhizome extract-treated RAW264.7 macrophage cells than t-BHP-incubated cells (p<0.05). These findings suggest that Rheum officinale Baillon rhizome extract has a potential as an excellent natural antioxidant agent.

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Antibacterial and antioxidant activities of Agaricus bisporus (J.E. Lange) imbach from Pakistan

Bangladesh Journal of Botany ◽

10.3329/bjb.v48i4.49055 ◽

2019 ◽

Vol 48 (4) ◽

pp. 1075-1081

Author(s):

Hafiz Muhammad Waqas ◽

Muhammad Akbar ◽

Muhammad Sajjad Iqbal

Keyword(s):

Free Radical ◽

Ethyl Acetate ◽

Pseudomonas Syringae ◽

Agaricus Bisporus ◽

Pathogenic Bacteria ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Gas Chromatography Mass Spectrometry ◽

Test Plant

In the present study, antibacterial and antioxidant (DPPH free radical scavenging activity) activity of an edible mushroom, Agaricus bisporus collected from Pakistan was investigated. In antibacterial assays, nhexane showed 4.83, 3.33, 16 and 3.33 mm inhibition zone diameter (IZD), chloroform showed 6.33, 16.17, 17 and 1.67 mm (IZD) while, ethyl acetate exhibited 30, 20, 19 and 28.67 mm (IZD) against four test plant pathogenic bacteria viz., Pseudomonas syringae Van Hall, Ralstonia solanacearum Yabuuchi et al. Xanthomonas axonopodis Dowson and Erwinia carotovora (Jones) Waldee, at 100 mg/ml concentration. Optical density (OD) was recorded at 517 nm. n-hexane extract showed 60.73, 47.6 and 33.05%, chloroform showed 89.97, 82.91 and 77.68% whereas, ethyl acetate exhibited 89.83, 65.82 and 23.59% free radical effects as compared to control. Gas chromatography mass spectrometry (GCMS) analysis showed the highest concentration of mono(2-ethylhexyl) phthalate.

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Antioxidant Activities and Total Phenolic Content of Malaysian Herbs as Components of Active Packaging Film in Beef Patties

Antioxidants ◽

10.3390/antiox8070204 ◽

2019 ◽

Vol 8 (7) ◽

pp. 204 ◽

Cited By ~ 5

Author(s):

Wan Amnin Wan Yahaya ◽

Noraziah Abu Yazid ◽

Nurul Aini Mohd Azman ◽

María Pilar Almajano

Keyword(s):

Total Phenolic Content ◽

Phenolic Content ◽

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Thiobarbituric Acid ◽

Active Packaging ◽

Total Phenolic ◽

Thiobarbituric Acid Reactive Substance ◽

Packaging Film

Active packaging containing natural extracts is a promising innovation to prolong the shelf life of perishable food. The objective of this work was to develop a bioactive edible film from semi-refined carrageenan (SRC) and glycerol (G) as plasticizer incorporated with natural extract. Five Malaysian herbs were evaluated to determine their total phenolic content (TPC) and antioxidant activities. The Persicaria minor (PM) extract demonstrated the highest TPC (1.629 mg GAE/L sample) and radical scavenging activity evaluated by the radicals 2,2’-azinobis [3-ethylbenzothiazoline-6-sulfonic acid] (27.166 mg TE/L sample), 2,2-diphenyl-1-picrylhydrazyl (719.89 mg eq. Trolox/L sample) and α,α′-Azodiisobutyramidine dihydrochloride (5.81 mg TE/L sample). Thus, PM extract was selected for active packaging film at concentrations of 0.4, 1.0 and 2.0% and compared with 0.4% Butylatedhydroxianisole in 2% SRC and 0.9% G film formulation. The meat patties were wrapped in the films and stored under refrigeration (4 ± 2 °C) for 14 days. The film with 2% PM exhibited significantly lower lipid deterioration analysed by the thiobarbituric acid reactive substance assay (p < 0.05) and small changes in % metmyoglobin value which indicated the minimum development of brown colour (p < 0.05). Hence, this film can be used as a packaging material to improve meat quality characteristics.

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Antioxidant and antibacterial activity of extracts, fractions and isolated substances from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don

Brazilian Journal of Pharmaceutical Sciences ◽

10.1590/s1984-82502010000400013 ◽

2010 ◽

Vol 46 (4) ◽

pp. 715-722 ◽

Cited By ~ 12

Author(s):

Cláudia Alexandra de Andrade ◽

João Luiz de Souza Carvalho ◽

Miriam Machado Cunico ◽

Ana Luísa Lacava Lordello ◽

Carmen Etsuko Kataoka Higaskino ◽

...

Keyword(s):

Antibacterial Activity ◽

Ethyl Acetate ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Ethanol Extract ◽

Thiobarbituric Acid ◽

Ethyl Acetate Fraction ◽

Total Phenol Content

The extracts and fractions from the flowers of A. podalyriifolia were analyzed previously for antibacterial activity using diffusion in disk, Antioxidant properties were evaluated by determining radical scavenging power (DPPH test) and total phenol content was measured (Folin method). The present study describes the in vitro antibacterial (determining minimum inhibitory concentration) and antioxidant activities (by thiobarbituric acid reactive species - TBARS method) for the ethanol extract, dichloromethane and ethyl acetate fractions and two flavanones (naringenin and 5-β-D-glycosyl-naringenin) isolated from the flowers of Acacia podalyriifolia A. Cunn. ex G. Don. The flavanones naringenin and 5-β-D-glycosyl-naringenin had not previously been obtained from this species. The most effective antibacterial activity was observed in the ethyl acetate fraction (MIC=0.25 mg mL-1 against Staphylococcus aureus ATCC 6538, MIC = 0.125 mg mL-1 against Staphylococcus epidermidis ATCC 12229, MIC=0.5 mg mL-1 against Streptococcus pyogenes ATCC 19615, Klebsiella pneumoniae ATCC 13883 and Proteus mirabilis ATCC 43071). The evaluated samples showed antioxidant activity on the TBARS test, especially for ethanol extract (1000 ppm), which was the most active (29.43% ± 0.65) followed by ethyl acetate fraction (1000 ppm, 24.84% ± 1,28), both demonstrating higher activity than that presented by ascorbic acid (1000 ppm, 21.73% ± 1.77), although lower than the BHT (1000 ppm 35.15% ± 3.42), both reference compounds. Naringenin and 5-β-D-glycosyl-naringenin demonstrated antioxidant action, but only naringenin inhibited the growth of gram-positive and gram-negative bacteria.

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Properties of Banana (Cavendish spp.) Starch Film Incorporated with Banana Peel Extract and Its Application

Molecules ◽

10.3390/molecules26051406 ◽

2021 ◽

Vol 26 (5) ◽

pp. 1406

Author(s):

Chanitda Taweechat ◽

Tipapon Wongsooka ◽

Saroat Rawdkuen

Keyword(s):

Uv Light ◽

Radical Scavenging Activity ◽

Radical Scavenging ◽

Water Vapor Permeability ◽

Thiobarbituric Acid ◽

Barrier Properties ◽

Banana Peel ◽

Vapor Permeability ◽

Peel Extract

The objective of this study was to develop an active banana starch film (BSF) incorporated with banana peel extract. We compared the film’s properties with commercial wrap film (polyvinyl chloride; PVC). Moreover, a comparison of the quality of minced pork wrapped during refrigerated storage (7 days at ±4 °C) was also performed. The BSF with different concentrations of banana peel extract (0, 1, 3, and 5 (%, w/v)) showed low mechanical properties (tensile strength (TS): 4.43–31.20 MPa and elongation at break (EAB): 9.66–15.63%) and water vapor permeability (3.74–11.0 × 10−10 g mm/sm2 Pa). The BSF showed low film solubility (26–41%), but excellent barrier properties to UV light. The BSF had a thickness range of 0.030–0.047 mm, and color attributes were: L* = 49.6–51.1, a* = 0.21–0.43, b* = 1.26–1.49. The BSF incorporated with banana peel extracts 5 (%, w/v) showed the highest radical scavenging activity (97.9%) and inhibitory activity of E. coli O157: H7. The BSF showed some properties comparable to the commercial PVC wrap film. Changes in qualities of minced pork were determined for 7 days during storage at ±4 °C. It was found that thiobarbituric acid reactive substances (TBARS) of the sample wrapped with the BSF decreased compared to that wrapped with the PVC. The successful inhibition of lipid oxidation in the minced pork was possible with the BSF. The BSF incorporated with banana peel extract could maintain the quality of minced pork in terms of oxidation retardation.

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Study of irradiated black pepper antioxidant activity changes

Czech Journal of Food Sciences ◽

10.17221/10656-cjfs ◽

2004 ◽

Vol 22 (SI - Chem. Reactions in Foods V) ◽

pp. S187-S190

Author(s):

M. Suhaj ◽

J. Rácová

Keyword(s):

Antioxidant Activity ◽

Antioxidant Activities ◽

Electron Paramagnetic Resonance Spectroscopy ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Thiobarbituric Acid ◽

Black Pepper ◽

Piper Nigrum ◽

Resonance Spectroscopy

Some antioxidant activities of extracts of irradiated black pepper (Piper nigrum) were evaluated. The ground black pepper was exposed to gamma-irradiation at doses from 5 to 30 kGy. The effect of irradiation on antioxidant properties of black pepper extracts was investigated by these methods: radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, determination of reducing power, thiobarbituric acid reactive substances assay and electron paramagnetic resonance spectroscopy (EPR). Irradiation resulted in a decrease of DPPH antiradical activity of black pepper extracts according to dose of irradiation. Reducing power was not changed by action of irradiation. Very important and sensitive differences in antioxidant activity of irradiated black pepper were investigated by EPR spectrometry and thiobarbituric acid method.

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Effect of Novel Synthesised Policosanyl Phenolates on Lipid Oxidation

Czech Journal of Food Sciences ◽

10.17221/530/2015-cjfs ◽

2016 ◽

Vol 34 (No. 5) ◽

pp. 414-421

Author(s):

Wang Zhiqiang ◽

Hwang Seung Hwan ◽

Lim Soon Sung

Keyword(s):

Linoleic Acid ◽

Lipid Oxidation ◽

Antioxidant Activities ◽

Radical Scavenging ◽

Thiobarbituric Acid ◽

Model System ◽

Total Oxidation ◽

High Lipid ◽

Inhibitory Activities ◽

High Lipid Peroxidation

Lipophilic derivatisation of phenolic acids could greatly improve their antioxidant activities and solubility in hydrophobic environments, broadening their applications in food, pharmaceutical, and cosmetic industries. In this study, we conducted enzymatic lipophilisation of eight phenolates with policosanols. Vinyl phenolates were used as intermediates to improve the efficiency of enzymatic lipophilisation; and the yields of policosanyl phenolates were in the range of 1.32–20.58%. The antioxidant activities of the resulting phenolipids were compared using 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) assay and linoleic acid peroxidation ferric thiocyanate assay. The synthesised policosanyl phenolates showed lower ABTS radical scavenging capacities (IC50s > 15 mM); whereas they showed high lipid peroxidation inhibitory activities (IC50s of peroxidation value < 0.25 mM). The lipid oxidation inhibitory activities of policosanol phenolates were further evaluated using the total oxidation value in a linoleic acid model system and the thiobarbituric acid reactive substances value in a cooked pork model system. Finally, policosanyl 4-hydroxybenzoate, policosanyl syringate, and policosanyl 4-hydroxyphenylacetate showed the highest inhibition effects on lipid oxidation and a potential for use as lipid antioxidants.

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Anti-fatigue Activity of Hovenia dulcis on a Swimming Mouse Model through the Inhibition of Stress Hormone Expression and Antioxidation

The American Journal of Chinese Medicine ◽

10.1142/s0192415x13500638 ◽

2013 ◽

Vol 41 (04) ◽

pp. 945-955 ◽

Cited By ~ 13

Author(s):

Chun-Soo Na ◽

Sun Young Yoon ◽

Jin Beom Kim ◽

Dae-Seung Na ◽

Mi-Sook Dong ◽

...

Keyword(s):

Physical Activity ◽

Antioxidant Activities ◽

Swimming Performance ◽

Stress Hormones ◽

Radical Scavenging ◽

Water Extract ◽

Thiobarbituric Acid ◽

Serum Levels ◽

Hot Water ◽

Hovenia Dulcis

Hovenia dulcis (H. dulcis) Thunb., which is distributed in Korea, China, and Japan, has been known to show hepatoprotective and free radical scavenging effects and enhance physical activity. Therefore, the objectives of this present study were to determine the anti-fatigue activity of hot-water extract from H. dulcis peduncle, and to find the reason why H. dulcis extract (HDE)-ingested mice had enhanced physical activity against swimming performance. The mice orally administrated with HDE (HDE-mice) dramatically enhanced their swimming time compared to the control mice. HDE significantly decreased serum levels of stress hormones, such as cortisol and adrenocorticotropic hormone (ACTH) in mice. The levels of thiobarbituric acid reactive substances (TBARS) were dramatically decreased in gastrocnemius muscle from both 100 mg/kg of HDE (LHDE) and 200 mg/kg of HDE (HHDE)-ingested mice compared to the control mice. The liver activities of superoxide dismutase (SOD) were significantly increased in HHDE-mice with increasing tendency in LHDE-mice. In addition, HHDE-mice significantly decreased the levels of blood glucose, total cholesterol (T-Chol), and triglyceride (TG). These results suggest that HDE had a significant anti-fatigue effect via its anti-stress and antioxidant activities, and thereby enhanced physical activity in swimming performance.

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