scholarly journals Study on the Effects of Microstructural Surfaces on the Attachment of Moving Microbes

Energies ◽  
2020 ◽  
Vol 13 (17) ◽  
pp. 4421
Author(s):  
Hongyue Yang ◽  
Ji Qian ◽  
Ming Yang ◽  
Chunxi Li ◽  
Hengfan Li ◽  
...  
Keyword(s):  
Shear Stress ◽  
Flow Direction ◽  
Width Ratio ◽  
Vortex Center ◽  
Low Velocity ◽  
Movement Model ◽  
Wall Area ◽  
Computational Fluid Dynamics Cfd ◽  
Microstructural Surface ◽  
Microbial Attachment

The research of marine antifouling is mainly conducted from the aspects of chemistry, physics, and biology. In the present work, the movement model of microorganisms along or against the flow direction on the microstructural surface was established. The model of globose algae with a diameter of 5 μm in the near-wall area was simulated by computational fluid dynamics (CFD), and the fluid kinematic characteristics and shear stress distribution over different-sized microstructures and in micropits were compared. Simulation results revealed that the increase of the β value (height to width ratio) was prone to cause vortexes in micropits. In addition, the closer the low-velocity region of the vortex center to the microstructural surface, the more easily the upper fluid of the microstructure slipped in the vortex flow and reduced the microbial attachment. Moreover, the shear stress in the micropit with a height and width of 2 μm was significantly higher than those in others; thus, microbes in this micropit easily fell off.

Abstract 572: Inhibition of MicroRNA-199a-5p Enhances Perfusion Recovery and Arteriogenesis Following Femoral Arterial Ligation

2016 ◽  
Vol 36 (suppl_1) ◽  
Author(s):  
Joshua L Heuslein ◽  
Richard J Price
Keyword(s):  
Shear Stress ◽  
Flow Direction ◽  
Arterial Occlusion ◽  
Locked Nucleic Acid ◽  
Reversed Flow ◽  
Arterial Ligation ◽  
Cross Sectional ◽  
Wall Area ◽  
Collateral Growth

We have recently reported that a subset of collateral artery segments in the mouse hindlimb are exposed to both a 2-fold increase in shear stress magnitude and reversed flow direction (“reversed” flow) following femoral arterial ligation (FAL). These segments experiencing a reversed flow subsequently undergo a disproportionate amplification of collateral growth (i.e. arteriogenesis) after FAL. Interestingly, a subset of microRNAs, including miR-199a-5p, are significantly down-regulated in endothelial cells exposed to a shear stress waveform biomimetic of this pro-arteriogenic “reversed flow” condition in-vitro. Here, we hypothesized that the inhibition of miR-199a-5p would lead to enhanced arteriogenesis and/or perfusion recovery following arterial occlusion. To test this hypothesis, Balb/c mice were treated with either anti-miR-199a-5p or non-targeting control locked-nucleic acid (LNA) immediately following FAL. Treatment efficacy was determined via RT-PCR for expression of miR-199a-5p and its downstream targets’ expression. Laser Doppler perfusion imaging and both whole mount and cross-sectional analysis of vascular casted gracilis muscles were used to assess vascular remodeling. We found that anti-miR-199a-5p LNA markedly reduced miR-199a-5p expression in the gracilis muscle by over 80%, 7 days post-FAL (n=4). By day 21 post-FAL, perfusion recovery was significantly improved in anti-miR-199a-5p treated mice (101.5±1.8% vs. 87.8±1.8%, p≤0.05, n=6). Moreover, both lumenal diameter (106.5±7.2μm vs. 78.4±2.9 μm, p≤0.0001, n=6) and wall area (1770±147μm 2 vs. 1163±41μm 2 , p≤0.0001, n=6) were significantly enhanced in anti-miR-199a-5p treated mice compared to controls. We therefore conclude that the inhibition of miR-199a-5p leads to enhanced perfusion recovery and arteriogenesis following arterial occlusion and could represent a novel strategy for therapeutic arteriogenesis.

scholarly journals Three-Dimensional Numerical Simulations and Antifouling Mechanism of Microorganisms on Microstructured Surfaces

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 319
Author(s):  
Hongyue Yang ◽  
Songling Wang ◽  
Chunxi Li ◽  
Hengfan Li
Keyword(s):  
Shear Stress ◽  
Flow Velocity ◽  
Vortex Flow ◽  
Flow Direction ◽  
Stress Gradient ◽  
Three Dimensional ◽  
Low Flow ◽  
Shear Stresses ◽  
Wall Area ◽  
Microstructured Surface

As marine biofouling seriously affects the development and utilization of oceans, the antifouling technology of microstructured surface has become a research hotspot due to its green and environmentally friendly advantages. In the present research, the motion models of microorganisms on the surfaces of five rectangular micropits, in co-current and counter-current flow direction, were established. Dynamic mesh technology was used to simulate the movements of microorganisms with different radii in the near-wall area, and the fluid kinematics and shear stress distributions in different-sized micropits were compared. Furthermore, moving microorganisms were included in the three-dimensional microstructure model to achieve the real situation of biofouling. Simulation results revealed that the vortex flow velocity in the micropits increased with the increase of the inlet flow velocity and the existence of the vortex flow effectively reduced the formation of conditioning layers in the micropits. In the downstream and countercurrent directions, the average shear stresses on the wall decreased with the increase of the micropit depth and width, and the shear stress on the inner wall of the Mp1 micropit (a patterned surface arranged with cubes of 2 µm × 2 µm × 2 µm) was found to be the largest. A low shear stress region with a low flow velocity was formed around microorganisms in the process of approaching the microstructured surface. The shear stress gradient of micro-ridge steps increased with the approach of microorganisms, indicating that microridge edges had a better effect on reducing microbial attachment.

Efficiency prediction for storage chambers using computational fluid dynamics

1996 ◽  
Vol 33 (9) ◽  
pp. 163-170 ◽  
Author(s):  
Virginia R. Stovin ◽  
Adrian J. Saul
Keyword(s):  
Fluid Dynamics ◽  
Computational Fluid Dynamics ◽  
Shear Stress ◽  
Particle Tracking ◽  
Critical Value ◽  
Complex Geometries ◽  
Bed Shear Stress ◽  
Breadth Ratio ◽  
Computational Fluid Dynamics Cfd ◽  
Simulated Flow

Research was undertaken in order to identify possible methodologies for the prediction of sedimentation in storage chambers based on computational fluid dynamics (CFD). The Fluent CFD software was used to establish a numerical model of the flow field, on which further analysis was undertaken. Sedimentation was estimated from the simulated flow fields by two different methods. The first approach used the simulation to predict the bed shear stress distribution, with deposition being assumed for areas where the bed shear stress fell below a critical value (τcd). The value of τcd had previously been determined in the laboratory. Efficiency was then calculated as a function of the proportion of the chamber bed for which deposition had been predicted. The second method used the particle tracking facility in Fluent and efficiency was calculated from the proportion of particles that remained within the chamber. The results from the two techniques for efficiency are compared to data collected in a laboratory chamber. Three further simulations were then undertaken in order to investigate the influence of length to breadth ratio on chamber performance. The methodology presented here could be applied to complex geometries and full scale installations.

scholarly journals Significance of Slippage and Electric Field in Mucociliary Transport of Biomagnetic Fluid

Lubricants ◽  
2021 ◽  
Vol 9 (5) ◽  
pp. 48
Author(s):  
Sufian Munawar
Keyword(s):  
Shear Stress ◽  
Flow Direction ◽  
Pressure Rise ◽  
Physical Parameters ◽  
Slippage Effect ◽  
Channel Surface ◽  
Electro Osmosis ◽  
Biomagnetic Fluid ◽  
Velocity Pressure ◽  
Metachronal Waves

Shear stress at the cilia wall is considered as an imperative factor that affects the efficiency of cilia beatings as it describes the momentum transfer between the fluid and the cilia. We consider a visco-inelastic Prandtl fluid in a ciliated channel under electro-osmotic pumping and the slippage effect at cilia surface. Cilia beating is responsible for the stimulation of the flow in the channel. Evenly distributed cilia tend to move in a coordinated rhythm to mobilize propulsive metachronal waves along the channel surface by achieving elliptic trajectory movements in the flow direction. After using lubrication approximations, the governing equations are solved by the perturbation method. The pressure rise per metachronal wavelength is obtained by numerically integrating the expression. The effects of the physical parameters of interest on various flow quantities, such as velocity, pressure gradient, pressure rise, stream function, and shear stress at the ciliated wall, are discussed through graphs. The analysis reveals that the axial velocity is enhanced by escalating the Helmholtz–Smoluchowski velocity and the electro-osmosis effects near the elastic wall. The shear stress at the ciliated boundary elevates with an increase in the cilia length and the eccentricity of the cilia structure.

Exploring wall shear stress spatiotemporal heterogeneity in coronary arteries combining correlation-based analysis and complex networks with computational hemodynamics

2020 ◽  
Vol 234 (11) ◽  
pp. 1209-1222 ◽  
Author(s):  
Karol Calò ◽  
Giuseppe De Nisco ◽  
Diego Gallo ◽  
Claudio Chiastra ◽  
Ayla Hoogendoorn ◽  
...  
Keyword(s):  
Shear Stress ◽  
Wall Shear Stress ◽  
Complex Networks ◽  
Coronary Arteries ◽  
Early Stage ◽  
Flow Direction ◽  
Computational Hemodynamics ◽  
Time Histories ◽  
Wall Shear

Atherosclerosis at the early stage in coronary arteries has been associated with low cycle-average wall shear stress magnitude. However, parallel to the identification of an established active role for low wall shear stress in the onset/progression of the atherosclerotic disease, a weak association between lesions localization and low/oscillatory wall shear stress has been observed. In the attempt to fully identify the wall shear stress phenotype triggering early atherosclerosis in coronary arteries, this exploratory study aims at enriching the characterization of wall shear stress emerging features combining correlation-based analysis and complex networks theory with computational hemodynamics. The final goal is the characterization of the spatiotemporal and topological heterogeneity of wall shear stress waveforms along the cardiac cycle. In detail, here time-histories of wall shear stress magnitude and wall shear stress projection along the main flow direction and orthogonal to it (a measure of wall shear stress multidirectionality) are analyzed in a representative dataset of 10 left anterior descending pig coronary artery computational hemodynamics models. Among the main findings, we report that the proposed analysis quantitatively demonstrates that the model-specific inlet flow-rate shapes wall shear stress time-histories. Moreover, it emerges that a combined effect of low wall shear stress magnitude and of the shape of the wall shear stress–based descriptors time-histories could trigger atherosclerosis at its earliest stage. The findings of this work suggest for new experiments to provide a clearer determination of the wall shear stress phenotype which is at the basis of the so-called arterial hemodynamic risk hypothesis in coronary arteries.

The Effect of Bench Arrangements on the Natural Ventilation of a Multispan Greenhouse

2013 ◽  
Author(s):  
Sunita Kruger ◽  
Leon Pretorius
Keyword(s):  
Fluid Dynamics ◽  
Natural Ventilation ◽  
Three Dimensional ◽  
Two Dimensional ◽  
Low Velocity ◽  
Lower Velocity ◽  
Cfd Models ◽  
Temperature Distributions ◽  
Computational Fluid Dynamics Cfd ◽  
Plant Level

In this paper, the influence of various bench arrangements on the microclimate inside a two-span greenhouse is numerically investigated using three-dimensional Computational Fluid Dynamics (CFD) models. Longitudinal and peninsular arrangements are investigated for both leeward and windward opened roof ventilators. The velocity and temperature distributions at plant level (1m) were of particular interest. The research in this paper is an extension of two-dimensional work conducted previously [1]. Results indicate that bench layouts inside the greenhouse have a significant effect on the microclimate at plant level. It was found that vent opening direction (leeward or windward) influences the velocity and temperature distributions at plant level noticeably. Results also indicated that in general, the leeward facing greenhouses containing either type of bench arrangement exhibit a lower velocity distribution at plant level compared to windward facing greenhouses. The latter type of greenhouses has regions with relatively high velocities at plant level which could cause some concern. The scalar plots indicate that more stagnant areas of low velocity appear for the leeward facing greenhouses. The windward facing greenhouses also display more heterogeneity at plant level as far as temperature is concerned.

CFD SIMULATION OF SHEAR STRESS AND SECONDARY FLOWS IN URETHRA

2007 ◽  
Vol 19 (02) ◽  
pp. 117-127 ◽  
Author(s):  
Yang-Yao Niu ◽  
Ding-Yu Chang
Keyword(s):  
Shear Stress ◽  
Cfd Simulation ◽  
Three Dimensional ◽  
Secondary Flows ◽  
Normal Female ◽  
Urinary System ◽  
Stress Distributions ◽  
Computational Fluid Dynamics Cfd ◽  
Peak Value ◽  
Lower Urinary

In this work, a preliminary numerical simulation of the lower urinary system using Computational Fluid Dynamics (CFD) is performed. Very few studies have been done on the simulation of three-dimensional urine through the lower urinary system. In this study, a simplified lower urinary model with rigid body assumption is proposed. The distributions of urine flow velocity, wall pressure and shear stress along the urethra are simulated based on MRI scanned uroflowmetry of a normal female. Numerical results show that violent secondary flows appear on the cross surface near the end of the urethra when the inflow rate is increased. The oscillative variation of pressure and shear stress distributions are found around the beginning section of the urethra when flow rate is at the peak value.

scholarly journals Numerical Analysis of Unsteady Laminar Flow past a Pentagonal Obstacle

2021 ◽  
Vol 10 (2) ◽  
pp. 968-974
Keyword(s):  
Laminar Flow ◽  
Flow Direction ◽  
Rectangular Channel ◽  
Time Frame ◽  
Two Dimensional ◽  
Fluid Inertia ◽  
Time Step ◽  
The Face ◽  
Computational Fluid Dynamics Cfd ◽  
Current Article

The current article dispenses the numerical investigation of a two dimensional unsteady laminar flow of incompressible fluid passing a regular pentagonal obstacle in an open rectangular channel. The centre of attention of this work is the comparison of drag coefficients estimated for two distinct cases based on the orientation of face and corner of an obstacle against the flow direction. The numerical results shows that the corner – oriented obstacle bring about 42% larger value of drag coefficient at Re = 500 than face – oriented obstacle. The substantial growth in the expanse of vortex behind obstacle (presented as a function of fluid inertia 25 < Re < 500) is analyzed through the contours and streamline patterns of velocity field. The two eddies in the downstream become entirely unsymmetrical at Re = 500 for both the cases, whereas; the flow separation phenomena occurs a bit earlier in the face – oriented case at Re = 250. Two dimensional Pressure – Based – Segregated solver is employed to model the governing equations written in velocity and pressure fields. The numerical simulations of unsteady flow are presented for 50 seconds time frame with time step 0.01 by using one of the best available commercial based Computational Fluid Dynamics (CFD) software, ANSYS 15.0.

Effect of shear stress upon localization of the Golgi apparatus and microtubule organizing center in isolated cultured endothelial cells

1993 ◽  
Vol 104 (4) ◽  
pp. 1145-1153 ◽  
Author(s):  
D.E. Coan ◽  
A.R. Wechezak ◽  
R.F. Viggers ◽  
L.R. Sauvage
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Cell Migration ◽  
Golgi Apparatus ◽  
Flow Direction ◽  
Microtubule Organizing Center ◽  
Directed Cell Migration ◽  
Time Period ◽  
Significant Difference ◽  
Organizing Center

Despite substantial evidence to suggest that directed cell migration is dependent upon positioning of the Golgi apparatus (GA) and the microtubule organizing center (MTOC), some controversy exists about whether such a relationship is relevant to endothelial cells under flow. The present study was undertaken to provide an indepth investigation of the relationship between shear stress, GA/MTOC localization, cell migration and nuclear position. Bovine carotid endothelial cells were exposed to 22 or 88 dynes/cm2 for 0.5, 2, 8 or 24 h, and localization of their GA/MTOCs was determined relative to the direction of flow. In no-flow control specimens, (0, 0.5, 2, 8 and 24 h) there was no change in the equally distributed GA/MTOCs. In contrast, during the first 8 h at 88 dynes/cm2 and by 2 h at 22 dynes/cm2 there was a significant increase in the number of cells with GA/MTOCs localized upstream to flow direction. The effect was temporary, however, and by 24 h there was no significant difference between the no-flow, 22 and 88 dynes/cm2 specimens. Analysis of GA/MTOC localization with respect to the direction of cell migration determined that 72.5% of no-flow cells possessed GA/MTOCs localized to the sides of nuclei nearest the direction of migration. In contrast, 64% of the specimens shear stressed over the same time period had GA/MTOCs localized to the sides of nuclei opposite the direction of migration. These results suggest that positioning of the GA/MTOC in endothelial cells is not dependent completely upon the direction of migration.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Optimization of Velocity Flap Structures in High Sensitivity Macrofluidic Airflow Sensor

2018 ◽  
Vol 7 (4.27) ◽  
pp. 11
Author(s):  
Mohamad Dzulhelmy bin Amari ◽  
Muhamad Saifuddin b. Abdull Shukor ◽  
Sukarnur Che Abdullah
Keyword(s):  
High Sensitivity ◽  
Low Velocity Impact ◽  
Low Velocity ◽  
Velocity Impact ◽  
Fast Detection ◽  
Intelligent Control System ◽  
Highly Sensitive ◽  
Computational Fluid Dynamics Cfd ◽  
Airflow Sensor ◽  
Program Software

Automated reaction from the system is most important in fulfilling the requirement of the intelligent control system. Hence, many related studies regarding in developing the hardware of the system such as high sensitivity of the airflow sensor in detecting the changes either in user or the environment. The effect of the fast detection of the sensor through the high sensitivity of the airflow sensor have enable the system to identify and analyze the behavior of the user in higher accuracy compared to conventional system. Within the scope of airflow sensitivity, separation between two parts in the airflow sensor in altering the velocity impact have been inquired in purpose, while a few investigations in relations to determine the pressure contour of the main parts have been explored by application of using Computational Fluid Dynamics (CFD. This simulation is performed in the ANSYS program software. Thus, this study consequently intends to be focus on detection the high sensitivity of the airflow movement by distinguishing the high and low velocity impact. The optimization the airflow sensor in this study based on design parameter also done in order to design and develop a highly sensitive airflow sensor   

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