scholarly journals Limosilactobacillus fermentum SWP-AFFS02 Improves the Growth and Survival Rate of White Shrimp via Regulating Immunity and Intestinal Microbiota

Fermentation ◽  
2021 ◽  
Vol 7 (3) ◽  
pp. 179
Author(s):  
Bao-Hong Lee ◽  
Wei-Hsuan Hsu ◽  
You-Zuo Chen ◽  
Kung-Ting Hsu ◽  
Tzu-Ming Pan
Keyword(s):  
Survival Rate ◽  
Intestinal Microbiota ◽  
Pathogenic Bacteria ◽  
Cell Number ◽  
Health Condition ◽  
White Shrimp ◽  
Control Group ◽  
Granular Cells ◽  
Feed Conversion ◽  
Important Species

White shrimp Litopenaeus vannamei is an important species of farmed shrimp. Intestinal bacterial composition and immune activity play important roles in regulating the health condition of shrimp. Lactic acid bacteria Limosilactobacillus fermentum SWP-AFFS02 was isolated from the intestine of sea fish Rachycentron canadum, and the potential of its effect on growth, immunity, and intestinal microbiota of L. vannamei shrimp was investigated. Shrimps received feed with or without the addition of 8 log CFU/g L. fermentum SWP-AFFS02 thrice a day for 8 weeks. After 8-week treatment, weight gain, feed conversion rate, and survival rate of shrimp were greater in the L. fermentum SWP-AFFS02-feed group than in the control group. L. fermentum SWP-AFFS02 treatment increased the number of granular cells and semi-granular cells and decreased hyaline cell number when compared to the control group. L. fermentum SWP-AFFS02 promoted prophenoloxidase (PO) activity through increasing immune-associated gene expression in the hepatopancreas of shrimp. In addition, administration of feed containing L. fermentum SWP-AFFS02 regulated intestinal microbiota via decreasing the ratio of pathogenic bacteria, such as Vibrionaceae and Enterobacteriaceae, in the intestine of shrimp. This study demonstrated that administration of L. fermentum SWP-AFFS02 effectively prevented infection of L. vannamei shrimp by regulating intestinal microbiota and enhancing immunity in shrimp to increase the growth and improve their health status, which acted as a probiotic and provided beneficial effects on shrimp.

scholarly journals Dietary Cold Pressed Watercress and Coconut Oil Mixture Enhances Growth Performance, Intestinal Microbiota, Antioxidant Status, and Immunity of Growing Rabbits

Animals ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 212 ◽  
Author(s):  
Mahmoud Alagawany ◽  
Mohamed Abd El-Hack ◽  
Adham Al-Sagheer ◽  
Mohammed Naiel ◽  
Islam Saadeldin ◽  
...  
Keyword(s):  
Intestinal Microbiota ◽  
Antioxidant Status ◽  
Pathogenic Bacteria ◽  
Coconut Oil ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Control Group ◽  
Feed Conversion ◽  
Live Body Weight ◽  
Cold Pressed

The present study assessed the effect of dietary supplementation with coconut oil (CNO), watercress oil (WCO), and their mixture as promoters of growth, antioxidant status, immunity, and intestinal microbiota in growing rabbits. A total of 120 rabbits were distributed into six groups (20 rabbits/group) receiving a basal diet without supplementation (G1) or diet supplemented with 2 g CNO (G2), 2 g WCO (G3), 0.5 g CNO plus 1.5 g WCO (G4), 1 g CNO plus 1 g WCO (G5), or 1.5 g CNO plus 0.5 g WCO/kg (G6). Live body weight and feed conversion ratio were significantly higher in the G4 and G5 groups than in the other groups. Superoxide dismutase activity and reduced glutathione concentration were significantly improved in the CNO or WCO diets. Supplemental CNO plus WCO at all tested levels produced the best lysozyme and complement 3 activities. Cecal lactobacilli, coliform, Enterobacteriaceae, and Clostridium spp. populations were lower in the group who received the 1 g CNO + 1 g WCO/kg diet than that in the control group. Dietary supplementation of 1 g CNO + 1 g WCO or 0.5 g CNO + 1.5 g WCO/kg had the potential to improve growth, feed utilization, antioxidant status, and immunity, and reduce cecal pathogenic bacteria in rabbits.

scholarly journals UTILIZATION OF MARINE FUNGAL Nodulisporium sp. KT29 METABOLITES TO IMPROVE THE PRODUCTION PERFORMANCE OF MARINE CULTURE OF WHITE SHRIMP

2017 ◽  
Vol 8 (2) ◽  
pp. 747
Author(s):  
Fazril Saputra ◽  
Dinamella Wahjuningrum ◽  
Kustiariyah Tarman ◽  
Irzal Effendi
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Production Performance ◽  
White Shrimp ◽  
Feed Conversion ◽  
Daily Growth Rate ◽  
Daily Growth ◽  
Marine Culture

<em>This study aimed to determine the best dose of administration of marine fungal <span style="text-decoration: underline;">Nodulisporium</span> sp. KT29 metabolites to improve the production performance of marine culture of white shrimp. The experimental animals used were white shrimp <span style="text-decoration: underline;">Litopenaeus</span> <span style="text-decoration: underline;">vannamei</span> post larvae 12, which were reared in the sea using floating net cages with a stocking density of 700 post larvae/net. Experimental design used was a completely randomized design consisting of 3 treatments with 3 replications; control without the administration of <span style="text-decoration: underline;">Nodulisporium</span> sp. KT29 metabolites, dietary of feed containing <span style="text-decoration: underline;">Nodulisporium</span> sp. KT29 metabolites at doses of 20 mL/kg of feed and 40 mL/kg of feed. The results showed that survival rate, absolute length growth, weight gain, daily growth rate and feed conversion ratio in treatment groups were better than the control (P&lt;0.05). The administration of marine fungal <span style="text-decoration: underline;">Nodulisporium</span> sp. KT29 metabolites at a dose of 20 mL/kg of feed could improve the production performance of white shrimp cultivated in the sea with survival rate, daily growth rate and feed conversion ratio: 66.61±6.94%, 20.18±0.39 %/day and 3.20±0.22, respectively.</em>

scholarly journals Alternate livelihood development for ‘Aila’ affected tribal people through aquaculture in Bali Island of the Sunderban, West Bengal, India

2017 ◽  
Vol 64 ◽  
Author(s):  
P. P. Chakrabarti ◽  
A. Ghosh ◽  
B. C. Mohapatra ◽  
N. K. Barik ◽  
A. Das ◽  
...  
Keyword(s):  
Heterotrophic Bacteria ◽  
C:N Ratio ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Control Group ◽  
Natural Food ◽  
Feed Conversion ◽  
Percentage Mortality ◽  
Cumulative Percentage

The concept of “biofloc technology” is changing the facet of intensive aquaculture with scope to attain high productivity in a sustainable manner. In biofloc, dense heterotrophic bacterial community is developed through C:N ratio manipulation, where the system becomes bacterial dominated rather than algae dominated and takes care of the wastes generated through in situ bioremediation. Protein is utilised in two ways; as feed for the shrimp and as microbial floc when the heterotrophic microbes convert the nitrogenous wastes into protein. It also promises a healthy rearing system, which is increasingly identified as one possible solution for disease problems especially those striking at early stages. The purpose of this study was to evaluate the effect of biofloc and periphyton technology (BPT) on the growth and immunomodulatory performance of Pacific white shrimp Penaeus vannamei during nursery and grow-out culture. The experimental BPT treatments with three tier substrate system with molasses as carbohydrate (CHO) source were compared with the conventional autotrophic system. The immunomodulation and cumulative percentage mortality upon challenge with pathogenic strain of Vibrio parahaemolyticus were assessed in the reared animals. We have successfully demonstrated the BPT based nursery and growout systems for P. vannamei with the advantage of providing significantly (p<0.05) better growth (27.6% improvement in average body weight, ABW) and feed utilisation (31% improvement in feed conversion ratio, FCR). A production level of 4-4.5 kg m-3 of water was achieved through this BPT system registering a significant improvement over the conventional system (p<0.05). The cumulative percentage mortality following pathogen challenge was significantly lower (p<0.05) in the biofloc grown shrimps compared to that of the control group, thus showing better resistance to pathogenic challenge. Furthermore, the biofloc reared shrimp did exhibit significant improvement in non-specific immune response in terms of serum phenoloxidase activity and total haemocyte counts possibly suggesting potential immunostimulatory role of the biofloc associated heterotrophic bacteria. This eco-based technology as revealed through our studies brings substantial improvement in productivity, minimising water requirement, recycling in situ nutrients and organic matter in turn improving farm biosecurity, augmentation of natural food, improvement of FCR and better health of the cultured shrimp.

scholarly journals UTILIZATION OF MARINE FUNGAL Nodulisporium sp. KT29 METABOLITES TO IMPROVE THE PRODUCTION PERFORMANCE OF MARINE CULTURE OF WHITE SHRIMP

2017 ◽  
Vol 8 (2) ◽  
pp. 747-755 ◽  
Author(s):  
Fazril Saputra ◽  
Dinamella Wahjuningrum ◽  
Kustiariyah Tarman ◽  
Irzal Effendi
Keyword(s):  
Growth Rate ◽  
Survival Rate ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Production Performance ◽  
White Shrimp ◽  
Feed Conversion ◽  
Daily Growth Rate ◽  
Daily Growth ◽  
Marine Culture

This study aimed to determine the best dose of administration of marine fungal Nodulisporium sp. KT29 metabolites to improve the production performance of marine culture of white shrimp. The experimental animals used were white shrimp Litopenaeus vannamei post larvae 12, which were reared in the sea using floating net cages with a stocking density of 700 post larvae/net. Experimental design used was a completely randomized design consisting of 3 treatments with 3 replications; control without the administration of Nodulisporium sp. KT29 metabolites, dietary of feed containing Nodulisporium sp. KT29 metabolites at doses of 20 mL/kg of feed and 40 mL/kg of feed. The results showed that survival rate, absolute length growth, weight gain, daily growth rate and feed conversion ratio in treatment groups were better than the control (P<0.05). The administration of marine fungal Nodulisporium sp. KT29 metabolites at a dose of 20 mL/kg of feed could improve the production performance of white shrimp cultivated in the sea with survival rate, daily growth rate and feed conversion ratio: 66.61±6.94%, 20.18±0.39 %/day and 3.20±0.22, respectively.

scholarly journals Replacing Fish Meal with Defatted Insect Meal (Yellow Mealworm Tenebrio molitor) Improves the Growth and Immunity of Pacific White Shrimp (Litopenaeus vannamei)

Animals ◽  
2019 ◽  
Vol 9 (5) ◽  
pp. 258 ◽  
Author(s):  
Constant Motte ◽  
Alfredo Rios ◽  
Thomas Lefebvre ◽  
Hong Do ◽  
Morgane Henry ◽  
...  
Keyword(s):  
Pathogenic Bacteria ◽  
Fish Meal ◽  
Control Diet ◽  
Group Versus ◽  
Tenebrio Molitor ◽  
Pacific White Shrimp ◽  
White Shrimp ◽  
Feed Conversion ◽  
Feeding Trial ◽  
Yellow Mealworm

Recently, ecological and economic issues have affected fish meal (FM) supply, the main source of protein for shrimp. This triggered a search for alternative dietary protein sources for shrimp production. We studied the consequences of replacing FM with a defatted insect meal, ŸnMealTM (YM), comprised of yellow mealworm (Tenebrio molitor). Growth and immune parameters of juvenile Pacific white shrimp (Litopenaeus vannanmei) were compared after an eight-week feeding trial. Shrimp were kept in aquaria with densities of 60 and 40 shrimp/m2 and fed one of five diets in which a proportion of FM was replaced by YM. All diets were isoproteic, isoenergetic, and balanced in lysine and methionine. After the feeding trial, shrimp were challenged with pathogenic bacteria (Vibrio parahaemolyticus). Growth and feed conversion parameters improved when YM was included in shrimp diets; with the highest weight gain and best food conversion ratio (FCR) achieved when 50% of FM was replaced by YM versus the control diet that contained no YM (initial weight: 1.60 g/shrimp; growth: 5.27 vs. 3.94 g/shrimp; FCR 1.20 vs. 1.59). In challenged shrimp, mortality rates were significantly less among groups that received YM, with a 76.9% lower mortality rate in the 50% FM replacement group versus the control.

scholarly journals Metabolome and Microbiota Analysis Reveals the Conducive Effect of Pediococcus acidilactici BCC-1 and Xylan Oligosaccharides on Broiler Chickens

2021 ◽  
Vol 12 ◽  
Author(s):  
Yuqin Wu ◽  
Zhao Lei ◽  
Youli Wang ◽  
Dafei Yin ◽  
Samuel E. Aggrey ◽  
...  
Keyword(s):  
Propionic Acid ◽  
Broiler Chickens ◽  
Pathogenic Bacteria ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Control Group ◽  
Gut Health ◽  
Feed Conversion ◽  
Metabolome Analysis ◽  
Treatment Groups

Xylan oligosaccharides (XOS) can promote proliferation of Pediococcus acidilactic BCC-1, which benefits gut health and growth performance of broilers. The study aimed to investigate the effect of Pediococcus acidilactic BCC-1 (referred to BBC) and XOS on the gut metabolome and microbiota of broilers. The feed conversion ratio of BBC group, XOS group and combined XOS and BBC groups was lower than the control group (P &lt; 0.05). Combined XOS and BBC supplementation (MIX group) elevated butyrate content of the cecum (P &lt; 0.05) and improved ileum morphology by enhancing the ratio of the villus to crypt depth (P &lt; 0.05). The 16S rDNA results indicated that both XOS and BBC induced high abundance of butyric acid bacteria. XOS treatment elevated Clostridium XIVa and the BBC group enriched Anaerotruncus and Faecalibacterium. In contrast, MIX group induced higher relative abundance of Clostridiaceae XIVa, Clostridiaceae XIVb and Lachnospiraceae. Besides, MIX group showed lower abundance of pathogenic bacteria such as Campylobacter. Metabolome analysis showed that all the 3 treatment groups (XOS, BBC and MIX) showed lower concentrations of sorbitol and both XOS and BBC group had higher concentrations of pyridoxine levels than CT group. Besides, XOS and BBC groups enhanced the content of hydroxyphenyl derivatives 4-hydroxyphenylpyruvate 1 and 3-(3-hydroxyphenyl) propionic acid, respectively (P &lt; 0.05). Notably, MIX group enhanced both 4-hydroxyphenylpyruvate 1 and 3-(3-hydroxyphenyl) propionic acid (P &lt; 0.05). Thus, XOS and BBC may have a synergistic role to improve the performance of broilers by modulating gut microbiota and metabolome.

scholarly journals P0256RESEARCH OF GUT MACROBIOTA IN PATIENTS WITH CHRONIC KIDNEY DISEASE

2020 ◽  
Vol 35 (Supplement_3) ◽  
Author(s):  
Kamila Olimxanova
Keyword(s):  
Chronic Kidney Disease ◽  
Kidney Disease ◽  
Intestinal Microbiota ◽  
Inflammatory Markers ◽  
Pathogenic Bacteria ◽  
Laboratory Data ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Markers Of Inflammation ◽  
The Relationship

Abstract Background and Aims Currently, it is estimated that the intestinal microbiota has hundreds of species of bacteria. It is known that intestinal dysbiosis contributes to the development of many diseases, such as obesity, diabetes mellitus, cardiovascular diseases, including CKD. The question of the relationship between bacterial contamination and the process of endogenous inflammation in patients with chronic kidney disease of the pre-dialysis stages is not completely clear. The aim of the study the composition of the intestinal microbiota in fecal samples and the level of inflammatory markers (CRP, IL-6, leukocytes) in patients with pre-dialysis stages of CKD. Method 60 patients with CKD of the pre-dialysis stages (C2-C4) were examined. The diagnosis of CKD was made on the basis of clinical, instrumental, and laboratory data. To study the relationship between the composition of the intestinal microbiota and inflammation in patients with CKD, we studied markers of inflammation such as CRP and IL-6. CRP was determined in blood serum by a highly sensitive method on a Humareader Single analyzer, IL-6 by enzyme-linked immunosorbent assay on a solid-phase analyzer. eGFR was calculated using the formula CKD-EPI-2011. The control group consisted of 30 healthy subjects. Statistical processing was carried out using methods of variation statistics. The results were processed using Microsoft Exel 2002 & Statistica 6.0. Results The average age of patients was 59 years. Of these, 25 women (40%) and 35 (60%) men. The estimated glomerular filtration rate varied from 30 to 60 ml / min / 1.73 m2, which corresponded to the indicators of the pre-dialysis stages of CKD. Analysis of intestinal microbiota showed a deficiency of Bifidobacterium bacteria (&lt;108 CFU), and an increase in the number of Escherichia (&gt; 108 CFU) in patients with CKD of the pre-dialysis stages. According to the results of the study, in the group of patients with CKD, the level of inflammatory markers was higher (CRP-55%, IL-6-60%, leukocytes 62%) than in the control group (CRP-45%, IL-6-40%, leukocytes 38% ) However, in men, the IL-6 index was higher than in women. In patients with CKD with obesity, the titer of pathogenic bacteria and their spectrum showed the worst results, although unreliable. Conclusion All examined patients had dysbiosis of the 2nd degree. At the same time, an imbalance of the intestinal microbiota is combined with an increased level of CRP, IL-6, and white blood cells.

scholarly journals Allium Extract Implements Weaned Piglet’s Productive Parameters by Modulating Distal Gut Microbiota

Antibiotics ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 269
Author(s):  
Miguel Rabelo-Ruiz ◽  
Claudia Teso-Pérez ◽  
Juan Manuel Peralta-Sánchez ◽  
Juan José Ariza ◽  
Antonio Manuel Martín-Platero ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Pathogenic Bacteria ◽  
Alpha Diversity ◽  
Diversity Indices ◽  
Daily Weight Gain ◽  
Control Group ◽  
Growth Promoter ◽  
Feed Conversion ◽  
Negative Effects ◽  
Global Threat

Antimicrobial resistance (AMR) has risen as a global threat for human health. One of the leading factors for this emergence has been the massive use of antibiotics growth-promoter (AGPs) in livestock, enhancing the spread of AMR among human pathogenic bacteria. Thus, several alternatives such as probiotics, prebiotics, or phytobiotics have been proposed for using in animal feeding to maintain or improve productive levels while diminishing the negative effects of AGPs. Reducing the use of antibiotics is a key aspect in the pig rearing for production reasons, as well as for the production of high-quality pork, acceptable to consumers. Here we analyze the potential use of Allium extract as an alternative. In this study, weaned piglets were fed with Allium extract supplementation and compared with control and antibiotic (colistin and zinc oxide) treated piglets. The effects of Allium extract were tested by analyzing the gut microbiome and measuring different productive parameters. Alpha diversity indices decreased significantly in Allium extract group in caecum and colon. Regarding beta diversity, significant differences between treatments appeared only in caecum and colon. Allium extract and antibiotic piglets showed better values of body weight (BW), average daily weight gain (ADG), and feed conversion ratio (FCR) than control group. These results indicate that productive parameters can be implemented by modifying the gut microbiota through phytobiotics such as Allium extract, which will drive to drop the use of antibiotics in piglet diet.

scholarly journals Effects of Dietary Tributyrin on Growth Performance, Biochemical Indices, and Intestinal Microbiota of Yellow-Feathered Broilers

Animals ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 3425
Author(s):  
Li Gong ◽  
Gengsheng Xiao ◽  
Liwei Zheng ◽  
Xia Yan ◽  
Qien Qi ◽  
...  
Keyword(s):  
Growth Performance ◽  
Intestinal Microbiota ◽  
Relative Abundance ◽  
Size Analysis ◽  
Control Group ◽  
Feed Conversion ◽  
Principal Coordinates Analysis ◽  
Linear Discriminant ◽  
Principal Coordinates ◽  
Biochemical Indices

This study aimed to evaluate the effects of tributyrin on growth performance, biochemical indices and intestinal microbiota of yellow-feathered broilers. 360 one-day-old chicks were randomly allocated to three treatments with six replicates of 20 chicks each, including a normal control group (NC), an antibiotic group (PC), and a tributyrin (250 mg/kg) group (TB) for 63 days. The results showed that compared with the control, the feed conversion ratio (FCR) in the TB group decreased during the d22 to d42 (p < 0.05) and overall, the final weight and FCR of broilers tended to increase and decrease, respectively. Moreover, the TB group showed the highest creatine concentrations at the entire period (p < 0.05). TB treatment increased the Bacteroidetes relative abundance and decreased Firmicutes. Principal coordinates analysis yielded clear clustering of the three groups. Linear discriminant analysis effect size analysis found seven differentially abundant taxa in the TB group, including several members of Bacteroidedetes. The relative abundance of Eisenbergiella, Phascolarctobacterium, Megasphaera and Intestinimonas increased in tributyrin-treated broilers. Spearman correlation analysis identified a correlation between Eisenbergiella abundance and overall feed efficiency. These results demonstrated that tributyrin could improve the growth performance by modulating blood biochemical indices and the cecal microflora composition of broilers.

scholarly journals The Effects of Fish Feed Supplemented with Azolla Meal on the Growth Performance, Digestive Enzyme Activity, and Health Condition of Genetically-Improved Farmed Tilapia (Oreochromis niloticus)

2020 ◽  
Vol 20 (3) ◽  
pp. 1029-1045 ◽  
Author(s):  
Fawzy I. Magouz ◽  
Mahmoud A.O. Dawood ◽  
Mahmoud F.I. Salem ◽  
Ayman A.I. Mohamed
Keyword(s):  
Digestive Enzyme ◽  
Goblet Cells ◽  
Health Condition ◽  
The Body ◽  
Phagocytic Index ◽  
Control Group ◽  
Fish Feed ◽  
Feed Conversion ◽  
Final Body Weight ◽  
Genetically Improved

AbstractAzolla meal was included in fish feed at different levels (10%, 20%, and 30%) and was fed to genetically-improved farmed tilapia (GIFT) for 90 days. The obtained results demonstrated that the final body weight, weight gain, and specific growth rate decreased significantly in fish fed 30% Azolla (P<0.05), while tilapia fed 10% and 20% did not differ significantly from those of the control (P>0.05). However, the feed conversion ratio increased significantly in fish fed 30% Azolla (P<0.05), while tilapia fed 10% and 20% did not differ significantly from those of the control (P>0.05). The body proximate analysis, amylase, lipase, protease, blood phagocytic index, and phagocytic and lysozyme activity were not affected by the inclusion of Azolla in tilapia diets and remained similar to those of the control group (P>0.05). The villus length of the foregut was not significantly affected by Azolla inclusion in tilapia diets (P>0.05). In the midgut, the villus length significantly (P<0.05) increased in fish fed Azolla at 20% and 30% compared to the control, with no differences from those fed at 10% (P>0.05). The villus length significantly (P<0.05) increased in the hind gut in fish fed Azolla at 30 % compared to the control, with no differences from those fed at 10% and 20% (P>0.05). The mucosal length of the tilapia foregut significantly (P<0.05) increased in fish fed Azolla at 10% compared to the control, with no differences from those fed at 20% and 30% (P>0.05). In the foregut and hindgut, the number of goblet cells significantly increased in fish fed Azolla at 3% compared to the control, with no differences from those fed at 10% and 20% (P>0.05), while in the midgut, the number of goblet cells significantly (P<0.05) increased in fish fed Azolla at 20% and 30% compared to the control, with no differences from those fed at 10% (P>0.05). Feeding tilapia with Azolla resulted in normal hematological and biochemical functions, with insignificant differences for the measured parameters except for the red blood cell count, which significantly (P<0.05) increased in fish fed Azolla at 20% compared to the control, with no differences from those fed at 20% and 30%.

Sign in / Sign up

Export Citation Format

Share Document