scholarly journals Citrinin in Foods and Supplements: A Review of Occurrence and Analytical Methodologies

Foods ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 14
Author(s):  
Liliana J. G. Silva ◽  
André M. P. T. Pereira ◽  
Angelina Pena ◽  
Celeste M. Lino
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Mammalian Species ◽  
High Sensitivity ◽  
Extraction Methods ◽  
Animal Origin ◽  
Red Rice ◽  
Food Contaminants ◽  
Detection Techniques ◽  
Wide Range

Citrinin (CIT) deserves attention due to its known toxic effects in mammalian species and its widespread occurrence in food commodities, often along with ochratoxin A, another nephrotoxic mycotoxin. Human exposure, a key element in assessing risk related to food contaminants, depends upon mycotoxin contamination levels in food and on food consumption. Commercial supplements, commonly designated as red rice, usually used in daily diets in Asiatic countries due to their medicinal properties, may pose a health problem as a result of high CIT levels. In addition to the worldwide occurrence of CIT in foods and supplements, a wide range of several analytical and detection techniques with high sensitivity, used for evaluation of CIT, are reviewed and discussed in this manuscript. This review addresses the scientific literature regarding the presence of CIT in foods of either vegetable or animal origin, as well as in supplements. On what concerns analytical methodologies, sample extraction methods, such as shaking extraction and ultrasonic assisted extraction (UAE), clean-up methods, such as liquid-liquid extraction (LLE), solid phase extraction (SPE) and Quick, Easy, Cheap, Effective, Rugged and Safe (QuECHERS), and detection and quantification methods, such as thin layer chromatography (TLC), high performance liquid chromatography (HPLC), capillary electrophoresis (CE), biosensors, and ELISA, are also reviewed.

scholarly journals Pulsed Electromagnetic Cross-Well Exploration for Monitoring Permafrost and Examining the Processes of Its Geocryological Changes

Geosciences ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 60
Author(s):  
Viacheslav Glinskikh ◽  
Oleg Nechaev ◽  
Igor Mikhaylov ◽  
Kirill Danilovskiy ◽  
Vladimir Olenchenko
Keyword(s):  
High Performance ◽  
Geophysical Methods ◽  
Three Dimensional ◽  
High Sensitivity ◽  
Data Interpretation ◽  
Industrial Facilities ◽  
Wide Range ◽  
Vector Finite Element ◽  
A New Technique ◽  
Pulsed Electromagnetic

This paper is dedicated to the topical problem of examining permafrost’s state and the processes of its geocryological changes by means of geophysical methods. To monitor the cryolithozone, we proposed and scientifically substantiated a new technique of pulsed electromagnetic cross-well sounding. Based on the vector finite-element method, we created a mathematical model of the cross-well sounding process with a pulsed source in a three-dimensional spatially heterogeneous medium. A high-performance parallel computing algorithm was developed and verified. Through realistic geoelectric models of permafrost with a talik under a highway, constructed following the results of electrotomography field data interpretation, we numerically simulated the pulsed sounding on the computing resources of the Siberian Supercomputer Center of SB RAS. The simulation results suggest the proposed system of pulsed electromagnetic cross-well monitoring to be characterized by a high sensitivity to the presence and dimensions of the talik. The devised approach can be oriented to addressing a wide range of issues related to monitoring permafrost rocks under civil and industrial facilities, buildings, and constructions.

scholarly journals Optimization of Plant Extract Purification Procedure for Rapid Screening Analysis of Sixteen Phenolics by Liquid Chromatography

Separations ◽  
2021 ◽  
Vol 8 (2) ◽  
pp. 13
Author(s):  
Petra Ranušová ◽  
Ildikó Matušíková ◽  
Peter Nemeček
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Solid Phase ◽  
Low Cost ◽  
Sinapic Acid ◽  
Rapid Screening ◽  
Coumaric Acid ◽  
Laboratory Equipment ◽  
Wide Range ◽  
Methoxycinnamic Acid

A solid-phase extraction (SPE) procedure was developed for simultaneous monitoring of sixteen different phenolics of various polarity, quantified by high-performance liquid chromatography (HPLC). The procedure allowed screening the accumulation of intermediates in different metabolic pathways that play a crucial role in plant physiology and/or are beneficial for human health. Metabolites mostly involved in phenylpropanoid, shikimate, and polyketide pathways comprise chlorogenic acid, gentisic acid, vanillic acid, caffeic acid, protocatechuic acid, ferulic acid, rutin, quercetin, epicatechin, gallic acid, sinapic acid, p-coumaric acid, o-coumaric acid, vanillin; two rarely quantified metabolites, 2,5-dimethoxybenzoic acid and 4-methoxycinnamic acid, were included as well. The procedure offered low cost, good overall efficiency, and applicability in laboratories with standard laboratory equipment. SPE recoveries were up to 99.8% at various concentration levels. The method allowed for routine analysis of compounds with a wide range of polarity within a single run, while its applicability was demonstrated for various model plant species (tobacco, wheat, and soybean), as well as different tissue types (shoots and roots).

scholarly journals COMPARISON OF THREE EXTRACTION METHODS OF ALLOPURINOL IN URIC ACID HERBAL MEDICINE WITH HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY QUANTIFICATION

2021 ◽  
pp. 141-147
Author(s):  
RIMADANI PRATIWI ◽  
RASPATI D. MULYANINGSIH ◽  
NYI M. SAPTARINI
Keyword(s):  
Herbal Medicine ◽  
High Performance ◽  
Solid Phase ◽  
Extraction Methods ◽  
Liquid Extraction ◽  
Filtering Method ◽  
Liquid Liquid Extraction ◽  
Accuracy And Precision ◽  
Relative Standard ◽  
Parameters Of Accuracy

Objective: This study was aimed to understand and determine the effectiveness of allopurinol extraction in herbal medicine from three extraction methods based on parameters of accuracy and precision. Methods: The study consisted of three methods including dissolving and filtering, liquid-liquid extraction, and solid-phase extraction with mixed-mode cation exchanger (SPE-MCX). The procedures were carried out using NaOH and HCl in dissolving and filtering method; methanol, HCl, and ethyl acetate in liquid-liquid extraction; and NH4OH elution solvent in SPE-MCX. Results: The results showed that extraction effectiveness based on accuracy level was the dissolving and filtering method>SPE-MCX>liquid-liquid extraction with % recovery+SD of 91.314+2.903%, 87.533+4.950%, and 54.549+3.517%, respectively. The precision level was the dissolution and filtering method>SPE-MCX>liquid-liquid extraction based on % relative standard deviations (RSD) of 3.18%, 5.226%, and 6.446%, respectively. Conclusion: It can be concluded that the allopurinol extraction method with the highest effectiveness based on accuracy and precision parameters in herbal medicine is the dissolving and filtering method.

scholarly journals Design and Evaluation of LYSO/SiPM LIGHTENING PET Detector with DTI Sampling Method

Sensors ◽  
2020 ◽  
Vol 20 (20) ◽  
pp. 5820
Author(s):  
Zhenzhou Deng ◽  
Yushan Deng ◽  
Guandong Chen
Keyword(s):  
High Performance ◽  
Sampling Method ◽  
Average Energy ◽  
High Sensitivity ◽  
Operating Conditions ◽  
Optimum Operating ◽  
Time Interval ◽  
Silicon Photomultiplier ◽  
Experimental Conditions ◽  
Wide Range

Positron emission tomography (PET) has a wide range of applications in the treatment and prevention of major diseases owing to its high sensitivity and excellent resolution. However, there is still much room for optimization in the readout circuit and fast pulse sampling to further improve the performance of the PET scanner. In this work, a LIGHTENING® PET detector using a 13 × 13 lutetium-yttrium oxyorthosilicate (LYSO) crystal array read out by a 6 × 6 silicon photomultiplier (SiPM) array was developed. A novel sampling method, referred to as the dual time interval (DTI) method, is therefore proposed to realize digital acquisition of fast scintillation pulse. A semi-cut light guide was designed, which greatly improves the resolution of the edge region of the crystal array. The obtained flood histogram shown that all the 13 × 13 crystal pixels can be clearly discriminated. The optimum operating conditions for the detector were obtained by comparing the flood histogram quality under different experimental conditions. An average energy resolution (FWHM) of 14.3% and coincidence timing resolution (FWHM) of 972 ps were measured. The experimental results demonstrated that the LIGHTENING® PET detector achieves extremely high resolution which is suitable for the development of a high performance time-of-flight PET scanner.

scholarly journals Collagen Induced Arthritis in DBA/1J Mice Associates with Oxylipin Changes in Plasma

2015 ◽  
Vol 2015 ◽  
pp. 1-11 ◽  
Author(s):  
Min He ◽  
Eduard van Wijk ◽  
Ruud Berger ◽  
Mei Wang ◽  
Katrin Strassburg ◽  
...  
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Multiple Reaction Monitoring ◽  
Mice Model ◽  
Collagen Induced Arthritis ◽  
Multivariate Statistical ◽  
Mediators Of Inflammation ◽  
Wide Range ◽  
Liquid Chromatography Tandem Mass ◽  
Healthy Control

Oxylipins play important roles in various biological processes and are considered as mediators of inflammation for a wide range of diseases such as rheumatoid arthritis (RA). The purpose of this research was to study differences in oxylipin levels between a widely used collagen induced arthritis (CIA) mice model and healthy control (Ctrl) mice. DBA/1J male mice (age: 6-7 weeks) were selected and randomly divided into two groups, namely, a CIA and a Ctrl group. The CIA mice were injected intraperitoneally (i.p.) with the joint cartilage component collagen type II (CII) and an adjuvant injection of lipopolysaccharide (LPS). Oxylipin metabolites were extracted from plasma for each individual sample using solid phase extraction (SPE) and were detected with high performance liquid chromatography/tandem mass spectrometry (HPLC-ESI-MS/MS), using dynamic multiple reaction monitoring (dMRM). Both univariate and multivariate statistical analyses were applied. The results in univariate Student’st-test revealed 10 significantly up- or downregulated oxylipins in CIA mice, which were supplemented by another 6 additional oxylipins, contributing to group clustering upon multivariate analysis. The dysregulation of these oxylipins revealed the presence of ROS-generated oxylipins and an increase of inflammation in CIA mice. The results also suggested that the collagen induced arthritis might associate with dysregulation of apoptosis, possibly inhibited by activated NF-κB because of insufficient PPAR-γligands.

Serotonin and its N-Acetylated and Acidic Derivatives in Insect Brain as Determined by High-Performance Liquid Chromatography with Electrochemical Detection

1993 ◽  
Vol 28 (1) ◽  
pp. 16-24 ◽  
Author(s):  
R. Vieira ◽  
M. Aldegunde
Keyword(s):  
Analytical Method ◽  
High Performance ◽  
Amperometric Detection ◽  
High Sensitivity ◽  
Hplc Method ◽  
Insect Brain ◽  
Oxidative Deamination ◽  
Wide Range ◽  
Hydrodynamic Voltammetry

The determination of serotonin (5-HT), N-acetylserotonin (NAS) and 5-hydroxy-3-indoleacetic acid (5-HIAA) in single brains of two acridids (Paracinema tricolor and Oedipoda caerulescens) was accomplished using a HPLC method combined with amperometric detection. A hydrodynamic voltammetry approach was used to assess the identity of each peak by comparing the voltammograms of standards and those of samples. The analytical method gave satisfactory reproducibility and sensitivity, and detected levels of 5-HT, NAS and 5-HIAA as low as 29, 55 and 10 fmol, respectively. This high sensitivity together with the simplicity of sample processing make the present analytical method suitable for a wide range of studies concerning indoleamine analyses in the insect nervous system. In both acridids, 5-HT showed the largest quantities, while its derivatives occurred in extremely low amounts. The results suggest that N-acetylation of 5-HT is quantitatively preferred to oxidative deamination in both species (NAS levels were 4-fold those of 5-HIAA). The relative importance of each catabolic pathway is discussed as related to physiological and genetic aspects.

QUANTIFICATION OF XYLOPIC ACID AND CHROMATOGRAPHIC FINGERPRINT EVALUATIONS OF THE DRIED FRUITS OF XYLOPIA AETHIOPICA FROM FOUR AFRICAN COUNTRIES

2018 ◽  
Vol 15 (3) ◽  
pp. 18
Author(s):  
Raphael N. Alolga ◽  
Assogba G. Assanhou ◽  
Vitus Onoja
Keyword(s):  
High Performance ◽  
Solid Phase ◽  
Fungal Infections ◽  
Active Principle ◽  
Average Content ◽  
African Countries ◽  
Chromatographic Fingerprint ◽  
Dried Fruits ◽  
Detection Techniques ◽  
Xylopia Aethiopica

Dunal) A. Rich, (herein called XYA), family Annonaceae, commonly known as “Guinea pepper”, “Ethiopian pepper” or “Negro pepper”, are widely used in traditional African medicines to treat a wide array of diseases including malaria, fungal infections, rheumatism, arthritis, etc. Scientific investigations have ascribed the following activities to the fruits of XYA; anti-diabetic, anti-inflammatory, antimicrobial, antiplasmodial, analgesic, anti-nociceptive, anti-proliferative, spermatogenic and neuropharmacological effects. The main active principle reported is xylopic acid (XA), a kaurene diterpene. This study aimed to develop and validate a simple HPLC/UV (high performance liquid chromatography – ultraviolet detection) analytical method for the quantification of XA that can be reproduced in poor-resource settings where advanced analytical detection techniques such as HPLC-MS are unavailable. Materials and Methods: Thus in this study, a simple C18 solid-phase extraction (SPE) column-pretreatment ─ HPLC/UV analytical procedure was developed for the quantification of XA in the dried fruits of XYA from four African countries, Benin, Cameroon, Ghana and Nigeria. The samples of XYA from the four countries were assessed for similarities using chromatographic fingerprinting. Results: The HPLC method was validated for linearity, limits of detection and quantification, precision and accuracy. The samples of XYA from Cameroon were found to have the highest average content of XA while those from Benin had the lowest average content of XA. Conclusion: Using the chromatographic fingerprint evaluation, the similarities of the samples from the four countries to the reference chromatogram was in the order: Benin > Cameroon > Nigeria > Ghana. Key words: Xylopia aethiopica, xylopic acid, C18

scholarly journals High-Sensitivity Flexible Pressure Sensor-Based 3D CNTs Sponge for Human–Computer Interaction

Polymers ◽  
2021 ◽  
Vol 13 (20) ◽  
pp. 3465
Author(s):  
Jianli Cui ◽  
Xueli Nan ◽  
Guirong Shao ◽  
Huixia Sun
Keyword(s):  
Pressure Sensor ◽  
High Performance ◽  
Large Scale ◽  
Low Cost ◽  
Pressure Sensors ◽  
Chemical Vapor ◽  
High Sensitivity ◽  
Wearable Electronics ◽  
Wide Range ◽  
Flexible Pressure Sensors

Researchers are showing an increasing interest in high-performance flexible pressure sensors owing to their potential uses in wearable electronics, bionic skin, and human–machine interactions, etc. However, the vast majority of these flexible pressure sensors require extensive nano-architectural design, which both complicates their manufacturing and is time-consuming. Thus, a low-cost technology which can be applied on a large scale is highly desirable for the manufacture of flexible pressure-sensitive materials that have a high sensitivity over a wide range of pressures. This work is based on the use of a three-dimensional elastic porous carbon nanotubes (CNTs) sponge as the conductive layer to fabricate a novel flexible piezoresistive sensor. The synthesis of a CNTs sponge was achieved by chemical vapor deposition, the basic underlying principle governing the sensing behavior of the CNTs sponge-based pressure sensor and was illustrated by employing in situ scanning electron microscopy. The CNTs sponge-based sensor has a quick response time of ~105 ms, a high sensitivity extending across a broad pressure range (less than 10 kPa for 809 kPa−1) and possesses an outstanding permanence over 4,000 cycles. Furthermore, a 16-pixel wireless sensor system was designed and a series of applications have been demonstrated. Its potential applications in the visualizing pressure distribution and an example of human–machine communication were also demonstrated.

scholarly journals A Review of the Analytical Methods for the Determination of 4(5)-Methylimidazole in Food Matrices

Chemosensors ◽  
2021 ◽  
Vol 9 (11) ◽  
pp. 322
Author(s):  
Panagiota-Kyriaki Revelou ◽  
Marinos Xagoraris ◽  
Eleftherios Alissandrakis ◽  
Christos S. Pappas ◽  
Petros A. Tarantilis
Keyword(s):  
Mass Spectrometry ◽  
Analytical Methods ◽  
High Performance ◽  
Water Solubility ◽  
Solid Phase ◽  
Sample Pretreatment ◽  
Extraction Methods ◽  
Exchange Chromatography ◽  
Food Matrices

4(5)-Methylimidazole (4(5)MEI) is a product of the Maillard reaction between sugars and amino acids, which occurs during the thermal processing of foods. This compound is also found in foods with caramel colorants additives. Due to its prevalence in foods and beverages and its potent carcinogenicity, 4(5)MEI has received federal and state regulatory agency attention. The aim of this review is to present the extraction procedures of 4(5)MEI from food matrices and the analytical methods for its determination. Liquid and gas chromatography coupled with mass spectrometry are the techniques most commonly employed to detect 4(5)MEI in food matrices. However, the analysis of 4(5)MEI is challenging due to the high polarity, water solubility, and the absence of chromophores. To overcome this, specialized sample pretreatment and extraction methods have been developed, such as solid-phase extraction and derivatization procedures, increasing the cost and the preparation time of samples. Other analytical methods for the determination of 4(5)MEI, include capillary electrophoresis, paper spray mass spectrometry, micellar electrokinetic chromatography, high-performance cation exchange chromatography, fluorescence-based immunochromatographic assay, and a fluorescent probe.

scholarly journals A miniaturized sensing device with ionically imprinted nanostructured films for the ultrasensitive detection of ions

2021 ◽  
Author(s):  
Antonio Ruiz ◽  
Xun Cao ◽  
Yizhong Huang ◽  
Kwang-Leong Choy
Keyword(s):  
High Performance ◽  
Bulk Diffusion ◽  
High Sensitivity ◽  
Open Circuit ◽  
Chloride Ions ◽  
The Novel ◽  
Ion Sensors ◽  
Ion Imprinted ◽  
Wide Range ◽  
Biomedical Diagnosis

Abstract The detection of ions is essential for a wide range of applications including biomedical diagnosis, and environmental monitoring among others. However, current ion sensors are based on thick sensing films (typically 100 µm), requiring time-consuming preparations, and have a thermodynamic limit to their sensitivity of 59 mV.Log[C]-1. Such configuration hinders the development of high-performance ion sensors due to the inherent limitations of the bulk diffusion of ions inside sensors. Consequently, they cannot be applied for high-precision applications that require high sensitivity. Furthermore, the research of anion monitoring is hampered due to the limited availability of molecular receptors with acceptable performances. We overcome such limitations by using a 300 nm nanostructured sensing film based on a novel nanoporous ion imprinted core-shell silica/gold nanoparticulate sensing film. The novel sensing film was highly selective towards chloride ions when compared to other anions such as nitrate, sulphate and carbonate. Moreover, this nanostructured sensing film exhibited above 3-fold higher sensitivity (-186.4 mV.Log[C]-1) towards chloride ions when compared to commercial devices. Such breakthrough has led to the fabrication of the smallest and most sensitive reported anion sensor working on open circuit potentiometry, with an exceptional selectivity towards chloride ions.

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