Foodborne TiO2 Nanoparticles Induced More Severe Hepatotoxicity in Fructose-Induced Metabolic Syndrome Mice via Exacerbating Oxidative Stress-Mediated Intestinal Barrier Damage

The hazard of titanium dioxide nanoparticles (TiO2 NPs) in diseased population should be given focus due to the huge number of these NPs in foods and medicine. This study aimed to evaluate the stronger biological adverse effect of oral exposure to TiO2 NPs in a fructose-induced metabolic syndrome mouse model. Compared to the normal mice, low-dose (2 mg/kg) TiO2 NPs did not cause severe hepatotoxicity. However, high-dose (20 mg/kg) TiO2 NPs induced aggravated hepatic inflammation, fibrosis, and apoptosis, with substantial alteration of related biochemical parameters in the mouse model. Moreover, significantly increased Ti and lipopolysaccharide burden were observed in metabolic syndrome murine liver and serum, which possibly worsened the portend intestinal leakage. The expression of tight junction-related protein showed that TiO2 NPs induced further increase in serious intestinal permeability. The intestinal inflammatory and oxidative stress response in the model were also assessed. Results showed that TiO2 NPs caused more severe intestinal inflammatory injury by intensifying the oxidative stress in metabolic syndrome mice and then induced further liver injury. This work provides information on the insights into the toxic effect of TiO2 NPs in sub-healthy population.

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Internalization of Titanium Dioxide Nanoparticles Is Cytotoxic for H9c2 Rat Cardiomyoblasts

Molecules ◽

10.3390/molecules23081955 ◽

2018 ◽

Vol 23 (8) ◽

pp. 1955 ◽

Cited By ~ 13

Author(s):

Elizabeth Huerta-García ◽

Iván Zepeda-Quiroz ◽

Helen Sánchez-Barrera ◽

Zaira Colín-Val ◽

Ernesto Alfaro-Moreno ◽

...

Keyword(s):

Oxidative Stress ◽

Cell Cycle ◽

Cell Proliferation ◽

Cell Death ◽

Titanium Dioxide ◽

Titanium Dioxide Nanoparticles ◽

Membrane Integrity ◽

The Body ◽

Potential Health ◽

Tio2 Nps

Titanium dioxide nanoparticles (TiO2 NPs) are widely used in industry and daily life. TiO2 NPs can penetrate into the body, translocate from the lungs into the circulation and come into contact with cardiac cells. In this work, we evaluated the toxicity of TiO2 NPs on H9c2 rat cardiomyoblasts. Internalization of TiO2 NPs and their effect on cell proliferation, viability, oxidative stress and cell death were assessed, as well as cell cycle alterations. Cellular uptake of TiO2 NPs reduced metabolic activity and cell proliferation and increased oxidative stress by 19-fold measured as H2DCFDA oxidation. TiO2 NPs disrupted the plasmatic membrane integrity and decreased the mitochondrial membrane potential. These cytotoxic effects were related with changes in the distribution of cell cycle phases resulting in necrotic death and autophagy. These findings suggest that TiO2 NPs exposure represents a potential health risk, particularly in the development of cardiovascular diseases via oxidative stress and cell death.

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Food Allergy Adjuvant and Early Immune Effects of Oral Exposure to Known and Suspected Gut Barrier Disruptors

10.21203/rs.3.rs-109618/v1 ◽

2020 ◽

Author(s):

Elena Drønen ◽

Unni Nygaard ◽

Ellen Namork ◽

Hubert Dirven

Keyword(s):

Food Allergy ◽

Mouse Model ◽

Peanut Allergy ◽

Intestinal Barrier ◽

Oral Exposure ◽

Gut Barrier ◽

Interleukin 33 ◽

Early Markers ◽

Peanut Extract ◽

Early Effects

Abstract Background: Exposure to adjuvants with a food allergen has been shown to promote sensitization and development of food allergy in animal models. Barrier disrupting capacities have been suggested to be one mechanism of adjuvant action. In this study, we investigated how gut barrier disrupting compounds affected food allergy development in a mouse model of peanut allergy. Sensitization and clinical peanut allergy in C3H/HEOuJ mice were assessed after repeated oral exposure to peanut extract together with cholera toxin (CT), the mycotoxin deoxynivalenol (DON), house dust mite (HDM) or the pesticide glyphosate (GLY). In addition, we investigated early effects 4 to 48 hours after a single exposure to the compounds, by assessing markers of intestinal barrier permeability, alarmin production, intestinal epithelial responses, and local immune responses.Results: CT and DON exerted adjuvant effects on peanut allergy development assessed as clinical anaphylaxis in mice. The early markers were consistently only affected by DON, observed as increased IL-33 (interleukin 33) and thymic stromal lymphopoietin (TSLP) alarmin production in intestines and Interleukin 33 receptor ST2 in serum. DON also induced an inflammatory immune response in lymph node cells stimulated with lipopolysaccharide (LPS). HDM and GLY did not promote clinical food allergy and affected few (or none) of the early markers at the present doses.Conclusions: Oral exposure to CT and DON promoted development of clinical anaphylaxis in the peanut allergy mouse model. DON, but not CT, affected the early markers measured in the present study, indicating that DON and CT have different modes of action at the early stages of peanut sensitization.

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Comparison of Fibrate, Ezetimibe, Low- and High-Dose Statin Therapy for the Dyslipidemia of the Metabolic Syndrome in a Mouse Model

Angiology ◽

10.1177/0003319710387919 ◽

2011 ◽

Vol 62 (2) ◽

pp. 144-154 ◽

Cited By ~ 8

Author(s):

Kosmas I. Paraskevas ◽

Alkistis Pantopoulou ◽

Ioannis S. Vlachos ◽

George Agrogiannis ◽

Dimitrios G. Iliopoulos ◽

...

Keyword(s):

Metabolic Syndrome ◽

Mouse Model ◽

Statin Therapy ◽

High Dose ◽

The Metabolic Syndrome

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Pre-fibrotic Status in the Lung of a Mouse Model of Metabolic Syndrome: Implication of Oxidative Stress and Inflammation

Free Radical Biology and Medicine ◽

10.1016/j.freeradbiomed.2017.10.238 ◽

2017 ◽

Vol 112 ◽

pp. 155-156

Author(s):

Maria C Della Vedova ◽

Sandra E Gomez Mejiba ◽

Miguel W Fornes ◽

Nidia N Gomez ◽

Dario C Ramirez

Keyword(s):

Oxidative Stress ◽

Metabolic Syndrome ◽

Mouse Model

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Study of some toxicological aspects of titanium dioxide nanoparticles through oxidative stress, genotoxicity, and histopathology in tilapia, Oreochromis mossambicus

10.21203/rs.3.rs-388947/v1 ◽

2021 ◽

Author(s):

Khurram Shahzad ◽

Muhammad Naeem Khan ◽

Farhat Jabeen ◽

Abdul Shakoor Chaudhry ◽

Chaman Ara ◽

...

Keyword(s):

Oxidative Stress ◽

Titanium Dioxide ◽

Soft Tissues ◽

Titanium Dioxide Nanoparticles ◽

Oreochromis Mossambicus ◽

Nuclear Bodies ◽

Tio2 Nps ◽

Tail Movement ◽

Water Tanks ◽

Different Doses

Abstract Extensive use of nanotechnology in multiple commodities is raising concern about nanotoxicity and particularly. Particularly, many studies reported the health hazardous effects of titanium dioxide nanoparticles (TiO2-NPs). Study focuses on toxicity and accumulation of TiO2-NPs in tilapia (Oreochromis mossambicus). For this purpose, Tilapia were kept in water tanks, acclimatized for fourteen days, and treated with different doses of TiO2 nanoparticles 0, 0.5, 1.0, and 1.5 mg TiO2-NPs /L. Results revealed an increase in accumulation of TiO2-NPs with an increase in doses. Moreover, with higher dose (1.5 mg /L) gills had maximum levels compared to muscles and liver tissues whereas other doses showed different accumulation patterns. A significantly higher concentration of catalase, glutathione, and lipid peroxidation was recorded in gills (p < 0.05) and superoxide dismutase in the liver. Characteristics like thickening and fusion in lamellae, rupturing of filaments and hyperplasia of gills were also recorded. The phenomenon of increased necrosis and apoptosis in the liver was also noticed with increasing concentration of TiO2-NPs along with formation of sinusoid spaces and condensed nuclear bodies. Elevated values of olive tail movement and % tail DNA were also noticed with increased concentration of TiO2-NPs. This study concluded that TiO2-NPs produced oxidative stress by accumulation in soft tissues and induced pathology and genotoxicity.

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Tissue-specific oxidative stress and element distribution after oral exposure to titanium dioxide nanoparticles in rats

Nanoscale ◽

10.1039/d0nr05591c ◽

2020 ◽

Vol 12 (38) ◽

pp. 20033-20046

Author(s):

Zhangjian Chen ◽

Pai Zheng ◽

Shuo Han ◽

Jiahe Zhang ◽

Zejun Li ◽

...

Keyword(s):

Oxidative Stress ◽

Titanium Dioxide ◽

Titanium Dioxide Nanoparticles ◽

Element Distribution ◽

Oral Exposure ◽

Tissue Specific

The present study found that orally ingested TiO2 NPs induced tissue specific oxidative stress and imbalance of elements.

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Different Physiological and Biochemical Responses of Bamboo to the Addition of TiO2 NPs under Heavy Metal Toxicity

Forests ◽

10.3390/f12060759 ◽

2021 ◽

Vol 12 (6) ◽

pp. 759

Author(s):

Abolghassem Emamverdian ◽

Yulong Ding ◽

Farzad Mokhberdoran ◽

Muthusamy Ramakrishnan ◽

Zishan Ahmad ◽

...

Keyword(s):

Oxidative Stress ◽

Heavy Metal ◽

Antioxidant Capacity ◽

Plant Biomass ◽

Plant Protection ◽

Titanium Dioxide Nanoparticles ◽

High Concentration ◽

Positive Role ◽

Tio2 Nps ◽

The Impact

Bamboo forests cover a remarkable area of Chinese forestland. Recently, titanium dioxide nanoparticles (TiO2 NPs) have been used for plant protection against abiotic stress. In this study, an in vitro tissue culture experiment was conducted to determine the impact of titanium on plant tolerance to two different heavy metals (Cu and Pb). Bamboo plants (Arundinaria pygmaea L.) were grown using five concentrations of TiO2 NPS (0, 50, 80, 100, and 150 µM) without or with 100 µM Cu and 100 µM Pb for 30 days. The results showed that while Cu and Pb increased the generation of Reactive oxygen species (ROS) compounds in plants, TiO2 NP treatments played a positive role in reducing oxidative stress, as indicated by the decrease in ROS compounds, the extent of lipoperoxidation, and soluble proteins. On the other hand, the use of TiO2 NPs increased the total antioxidant capacity, chlorophyll content and general plant biomass. Moreover, the addition of TiO2 NPs significantly reduced Cu, and Pb accumulation in roots, stems, and shoots. We concluded that TiO2 NPs have the ability to reduce oxidative stress in plants by increasing the antioxidant capacity, improving the level of injury, and protecting cell membranes via reducing lipoperoxidation (reduction of Malondialdehyde (MDA) content). However, the results indicated that the efficiency of TiO2 NPs was related to the type and concentration of heavy metal, as TiO2 NPs were more effective for Cu than Pb. Additionally, a high concentration of TiO2 NPs resulted in the greatest enhancement in plant growth and development under heavy metal stress.

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Elimination of Oxidative Stress and Genotoxicity of Biosynthesized Titanium Dioxide Nanoparticles in Rats via Supplementation with Whey Protein-Coated Thyme Essential Oil

10.21203/rs.3.rs-398050/v1 ◽

2021 ◽

Author(s):

Mosaad Attia Abdel-Wahhab ◽

Aziza A. El-Nekeety ◽

Hagar E Mohammed ◽

Ola I. Elshafey ◽

Sekena H. Abdel-Aziem ◽

...

Keyword(s):

Oxidative Stress ◽

Essential Oil ◽

Zeta Potential ◽

Titanium Dioxide Nanoparticles ◽

Control Group ◽

High Dose ◽

Dependent Manner ◽

Bioactive Constituents ◽

Thyme Essential Oil ◽

Liver And Kidney

Abstract The green synthesis of metal nanoparticles is growing dramatically; however, the toxicity of these biosynthesized particles against living organisms is not fully explored. Therefore, this study was designed to synthesize and characterize TiO2-NPs, encapsulation and characterization thyme essential oil (ETEO), determination of the bioactive constituents of ETEO using GC-MS and evaluate their protective role against TiO2-NPs-induced the oxidative damage and genotoxicity in rats. Six groups of rats were treated orally for 30 days including the control group, TiO2-NPs (300 mg/kg b.w)-treated group, ETEO at low (50 mg/kg b.w) or high dose (100 mg/kg b.w)-treated groups TiO2-NPs plus ETEO at the two doses-treated groups. Blood and tissues were collected for different assays. The GC-MS results indicated the presence of 21 compounds belongs to phenols, terpene derivatives, and heterocyclic compounds. The synthesized TiO2-NPs were 45 nm tetragonal particles with a zeta potential of -27.34 mV; however, ETEO were 119 nm round particles with a zeta potential of -28.33 mV. TiO2-NPs administration disturbs the liver and kidney markers, lipid profile, cytokines, oxidative stress parameters, the apoptotic and antioxidant hepatic mRNA expression and induced histological alterations in the liver and kidney tissues. ETEO could improve all these parameters in a dose-dependent manner. It could be concluded that ETEO is a promising candidate for the protection against TiO2-NPs and can be applied safely in food applications.

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Protective Effect of Lactobacillus rhamnosus GG on TiO2 Nanoparticles-Induced Oxidative Stress Damage in the Liver of Young Rats

Nanomaterials ◽

10.3390/nano11030803 ◽

2021 ◽

Vol 11 (3) ◽

pp. 803

Author(s):

Penghui Nie ◽

Mengqi Wang ◽

Yu Zhao ◽

Shanji Liu ◽

Ling Chen ◽

...

Keyword(s):

Oxidative Stress ◽

Liver Toxicity ◽

Titanium Dioxide Nanoparticles ◽

Lactobacillus Rhamnosus ◽

Oral Intake ◽

Protective Effects ◽

Lactobacillus Rhamnosus Gg ◽

Tio2 Nps ◽

Young Rats ◽

Hematological Indicators

The potential toxicity of titanium dioxide nanoparticles (TiO2 NPs) to mammals has become a widespread concern. Young individuals exposed to TiO2 NPs have a higher risk than adults. In this study, the protective effects of Lactobacillus rhamnosus GG (LGG) on liver toxicity in young rats induced by TiO2 NPs were explored. Results show that the four-week-old rats that underwent LGG after the oral intake of TiO2 NPs could prevent weight loss, reduce hematological indicators (WBC and NEUT) and serum biochemical indicators (AST, ALT, AST/ALT, and ALP). Moreover, it alleviated the pathological damage of the liver (as indicated by the disordered hepatocytes, more eosinophilic, ballooning degeneration, and accompany with blood cells), but it did not reduce the Ti contents in the liver. In addition, RT-qPCR results indicated that LGG restored the expression of anti-oxidative stress-related genes, such as SOD1, SOD2, CAT, HO-1, GSH, GCLC, and GCLM in the liver. In summary, the hepatotoxicity of TiO2 NPs in young rats is closely related to oxidative stress, and the antioxidant effect of LGG might protect the harmful effects caused by TiO2 NPs.

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