Antimicrobial Activity and Prevention of Bacterial Biofilm Formation of Silver and Zinc Oxide Nanoparticle-Containing Polyester Surfaces at Various Concentrations for Use

Food contact surfaces are primary sources of bacterial contamination in food industry processes. With the objective of preventing bacterial adhesion and biofilm formation on surfaces, this study evaluated the antimicrobial activity of silver (Ag-NPs) and zinc oxide (ZnO-NPs) nanoparticle-containing polyester surfaces (concentration range from 400 ppm to 850 ppm) using two kinds of bacteria, Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli), and the prevention of bacterial biofilm formation using the pathogen Listeria monocytogenes. The results of antimicrobial efficacy (reductions ≥ 2 log CFU/cm2) showed that at a concentration of 850 ppm, ZnO-NPs were effective against only E. coli (2.07 log CFU/cm2). However, a concentration of 400 ppm of Ag-NPs was effective against E. coli (4.90 log CFU/cm2) and S. aureus (3.84 log CFU/cm2). Furthermore, a combined concentration of 850 ppm Ag-NPs and 400 ppm ZnO-NPs showed high antimicrobial efficacy against E. coli (5.80 log CFU/cm2) and S. aureus (4.11 log CFU/cm2). The results also showed a high correlation between concentration levels and the bacterial activity of Ag–ZnO-NPs (R2 = 0.97 for S. aureus, and R2 = 0.99 for E. coli). They also showed that unlike individual action, the joint action of Ag-NPs and ZnO-NPs has high antimicrobial efficacy for both types of microorganisms. Moreover, Ag-NPs prevent the biofilm formation of L. monocytogenes in humid conditions of growth at concentrations of 500 ppm. Additional studies under different conditions are needed to test the durability of nanoparticle containing polyester surfaces with antimicrobial properties to optimize their use.

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Synthesis and Characterization Silver, Zinc Oxide and Hybrid Silver/Zinc Oxide Nanoparticles for Antimicrobial Applications

Nano LIFE ◽

10.1142/s1793984414400030 ◽

2014 ◽

Vol 04 (01) ◽

pp. 1440003 ◽

Cited By ~ 16

Author(s):

Myisha Roberson ◽

Vijaya Rangari ◽

Shaik Jeelani ◽

Temesgen Samuel ◽

Clayton Yates

Keyword(s):

Zinc Oxide ◽

Zno Nanoparticles ◽

Biomedical Application ◽

Fungal Growth ◽

X Ray Diffraction ◽

Ag Nps ◽

Zno Nps ◽

E Coli ◽

Transmission Electron ◽

20 Nm

Silver ( Ag ) and zinc oxide ( ZnO ) are well known for both antimicrobial and pro-healing properties. Here, we present a novel method to synthesize Ag and ZnO nanoparticles (NPs), as well as hybrid Ag / ZnO NPs using a custom, temperature controlled microwave assisted technique. Microwave synthesis has been shown not only to enhance the rate of chemical reactions, but also in some cases to give higher product yields over thermal heating. The as-synthesized NPs were characterized by X-ray diffraction (XRD) to study the crystalline structure, composition and purity. Transmission electron microscopy (TEM) and energy dispersive spectroscopy (EDS) was used to study particle size, shape, composition and morphology. These results indicated that the as-prepared Ag NPs are spherical in shape and ~ 20 nm in sizes. The ZnO NPs are typically rod shaped and the particle sizes are ~ 20 nm in width and 100 nm in length. These NPs were tested for antibacterial and/or antifungal properties using disc diffusion assays. Results show microwave synthesized NPs inhibit growth of S. aureus, E. coli and C. albicans at 50 μ g/mL treatment concentration. Ag NPs were most effective in inhibiting bacterial and fungal growth at the concentrations tested followed by hybrid Ag / ZnO and ZnO nanoparticles. These results also suggest that the hybridization of ZnO to Ag NPs may reduce the toxicity of Ag NPs. Further studies are needed to understand the functional interaction between the two types of NPs and to improve their ability for biological or biomedical application.

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Research of antimicrobial efficacy of modern antiseptic agents based on decamethoxine and povidone-iodine

Perioperaciina Medicina ◽

10.31636/prmd.v2i1.1 ◽

2019 ◽

Vol 2 (1) ◽

pp. 6-10 ◽

Cited By ~ 1

Author(s):

Oleksandr Nazarchuk

Keyword(s):

Antimicrobial Activity ◽

Povidone Iodine ◽

Antimicrobial Properties ◽

Infectious Complications ◽

Antimicrobial Efficacy ◽

Gram Positive ◽

Gram Negative ◽

Clinical Strains ◽

E Coli ◽

Enterococcus Spp

Under conditions of wide increased resistance of pathogens of infectious complications to antimicrobial agents, a considerable attention is paid to the use of antiseptic drugs. The research of their antimicrobial efficacy remains valid for substantiation of the rational use. The purpose was to carry out a comparative study of the antimicrobial efficacy of medicinal antiseptic agents based on decamethoxine and povidone-iodine. In the study there was examined antimicrobial activity of mentioned antiseptics against 682 clinical strains of microorganisms (A. baumannii, S. aureus, P. aeruginosa, Enterococcus spp., E. coli, Enterobacter spp., K. pneumoniae, Proteus spp.), isolated from patients with infectious complications. Minimum inhibitory and bactericidal concentrations of 1–2– 10 % iodine, 0.02 % decamethoxine (decasan) were determined. Antimicrobial efficacy of medicines, based on studied antiseptics was evaluated with the use of an index of antiseptic activity, calculated by means of commonly used methods. The study revealed high bactericidal properties of decasan against clinical strains of S. aureus, Enterococcus spp., E. coli, K. pneumoniae and Enterobacter spp. The advantages of antimicrobial activity of decamethoxine-based antseptis (decasan, p < 0.001) were proved. Iodine has expressed antimicrobial properties against Enterococcus spp., S. aureus, A. baumannii, bacteria of the Enterobacteriаcеae family and P. aeruginosa. It is proven that the dissolution of povidone-iodine leads to the reduction of the antimicrobial efficacy of 2 % antiseptic solution. There was found inefficacy of 1 % povidone-iodine against infectious agents (p < 0.001). Thus, the leading Gram-positive (S. aureus, Enterococci) and Gram-negative pathogens (Escherichia, Klebsiella, Enterobacteria, Acinetobacteria, Pseudomonas) have a sensitivity to iodine and to domestic preparation based on decamethoxin 0.02 % (decasan), with a definite advantage of the antimicrobial properties of the latter to Gram-positive and some Gram-negative microorganisms (p < 0.001).

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Antimicrobial effects of airborne acoustic ultrasound and plasma activated water from cold and thermal plasma systems on biofilms

Scientific Reports ◽

10.1038/s41598-020-74504-z ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

Clémentine M. G. Charoux ◽

Apurva D. Patange ◽

Laura M. Hinds ◽

Jeremy C. Simpson ◽

Colm P. O’Donnell ◽

...

Keyword(s):

Thermal Plasma ◽

Biofilm Formation ◽

Synergistic Effects ◽

Antimicrobial Properties ◽

Bacterial Count ◽

Bacterial Biofilm ◽

Antimicrobial Effects ◽

E Coli ◽

And Control ◽

Plasma Activated Water

Abstract Bacterial biofilms are difficult to inactivate due to their high antimicrobial resistance. Therefore, new approaches are required for more effective bacterial biofilm inactivation. Airborne acoustic ultrasound improves bactericidal or bacteriostatic activity which is safe and environmentally friendly. While, plasma activated water (PAW) is attracting increasing attention due to its strong antimicrobial properties. This study determined efficacy of combined airborne acoustic ultrasound and plasma activated water from both cold and thermal plasma systems in inactivating Escherichia coli K12 biofilms. The application of airborne acoustic ultrasound (15 min) alone was significantly more effective in reducing E. coli counts in 48 and 72 h biofilms compared to 30 min treatment with PAW. The effect of airborne acoustic ultrasound was more pronounced when used in combination with PAW. Airborne acoustic ultrasound treatment for 15 min of the E. coli biofilm followed by treatment with PAW significantly reduced the bacterial count by 2.2—2.62 Log10 CFU/mL when compared to control biofilm treated with distilled water. This study demonstrates that the synergistic effects of airborne acoustic ultrasound and PAW for enhanced antimicrobial effects. These technologies have the potential to prevent and control biofilm formation in food and bio-medical applications.

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Disinfection of Fruits with Activated Water

JOURNAL OF ADVANCES IN AGRICULTURE ◽

10.24297/jaa.v10i0.8462 ◽

2019 ◽

Vol 10 ◽

pp. 1864-1872

Author(s):

Prof. Teodora P. Popova

Keyword(s):

Antimicrobial Activity ◽

Aqueous Solutions ◽

Antimicrobial Properties ◽

The Other ◽

Gram Negative Bacteria ◽

High Antimicrobial Activity ◽

Gram Negative ◽

E Coli ◽

Number Of Microorganisms ◽

Lower Effect

The effect of ionized aqueous solutions (anolytes and catholyte) in the processing of fruits (cherries, morellos, and strawberries) for decontamination has been tested. Freshly prepared analytes and catholyte without the addition of salts were used, as well as stored for 7 months anolytes, prepared with 0.5% NaCl and a combination of 0.5% NaCl and 0.5% Na2CO3. The anolyte prepared with a combination of 0.5% NaCl and 0.5% Na2CO3, as well as the anolyte obtained with 0.5% NaCl, exhibit high antimicrobial activity against the surface microflora of strawberries, cherries, and sour cherries. They inactivate E. coli for 15 minutes. The other species of the fam. Enterobacteriaceae were also affected to the maximum extent, as is the total number of microorganisms, especially in cherries and sour cherries. Even stored for 7 months, they largely retain their antimicrobial properties. Anolyte and catholyte, obtained without the addition of salts, showed a lower effect on the total number of microorganisms, but had a significant effect on Gram-negative bacteria, and especially with regard to the sanitary indicative E. coli.

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Zinc Oxide and Silver Nanoparticle Effects on Intestinal Bacteria

Materials ◽

10.3390/ma14102489 ◽

2021 ◽

Vol 14 (10) ◽

pp. 2489

Author(s):

Ami Yoo ◽

Mengshi Lin ◽

Azlin Mustapha

Keyword(s):

Electron Microscopy ◽

Zinc Oxide ◽

Morphological Changes ◽

Intestinal Bacteria ◽

Bacterial Cells ◽

Ag Nps ◽

Zno Nps ◽

Bifidobacterium Animalis ◽

Transmission Electron

The application of nanoparticles (NPs) for food safety is increasingly being explored. Zinc oxide (ZnO) and silver (Ag) NPs are inorganic chemicals with antimicrobial and bioactive characteristics and have been widely used in the food industry. However, not much is known about the behavior of these NPs upon ingestion and whether they inhibit natural gut microflora. The objective of this study was to investigate the effects of ZnO and Ag NPs on the intestinal bacteria, namely Escherichia coli, Lactobacillus acidophilus, and Bifidobacterium animalis. Cells were inoculated into tryptic soy broth or Lactobacilli MRS broth containing 1% of NP-free solution, 0, 12, 16, 20 mM of ZnO NPs or 0, 1.8, 2.7, 4.6 mM Ag NPs, and incubated at 37 °C for 24 h. The presence and characterization of the NPs on bacterial cells were investigated by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and energy-dispersive X-ray spectroscopy (EDS). Membrane leakage and cell viability were assessed using a UV-visible spectrophotometer and confocal electron microscope, respectively. Numbers of treated cells were within 1 log CFU/mL less than those of the controls for up to 12 h of incubation. Cellular morphological changes were observed, but many cells remained in normal shapes. Only a small amount of internal cellular contents was leaked due to the NP treatments, and more live than dead cells were observed after exposure to the NPs. Based on these results, we conclude that ZnO and Ag NPs have mild inhibitory effects on intestinal bacteria.

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Emerging investigator series: Chemical transformation of silver and zinc oxide nanoparticles in the simulated human tear fluids: Influence of biocorona

Environmental Science Nano ◽

10.1039/d1en00566a ◽

2021 ◽

Author(s):

Lingxiangyu Li ◽

Ashfeen Ubaid Khan ◽

Xiang Zhang ◽

Xiaoting Qian ◽

Yawei Wang

Keyword(s):

Silver Nanoparticles ◽

Zinc Oxide ◽

Chemical Transformation ◽

Zinc Oxide Nanoparticles ◽

Biological Fluids ◽

Rapid Development ◽

Oxide Nanoparticles ◽

Personal Care ◽

Ag Nps ◽

Zno Nps

With the rapid development of nanotechnology, personal care products with silver nanoparticles (Ag-NPs) or zinc oxide nanoparticles (ZnO-NPs) are being widely used because of their superior antibacterial efficacies. Biological fluids...

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Comparative Study on the Antimicrobial Efficacy of Methanolic Extract of Root, Callus and Fruit Extracts of Myxopyrum smilacifolium Blume

International Journal of Applied Sciences and Biotechnology ◽

10.3126/ijasbt.v2i4.11362 ◽

2014 ◽

Vol 2 (4) ◽

pp. 521-524

Author(s):

RP Praveen ◽

Ashalantha Nair

Keyword(s):

Antimicrobial Activity ◽

Methanolic Extract ◽

Fungal Strain ◽

Antimicrobial Efficacy ◽

Bacterial Strains ◽

E Coli ◽

Root Extracts ◽

Fruit Extracts ◽

Root Callus ◽

And Staphylococcus Aureus

The aim of the present study was to compare the antimicrobial efficacy of methanolic extract of root, callus and fruit of Myxopyrum smilacifolium Blume. Antimicrobial activity was tested using agar well diffusion with four bacterial strains viz: Escherechia coli, Enterococcus faecalis, Bacillus subtilis and Staphylococcus aureus of which E. coli alone was gram negative. The fungal strain employed was Candida albicans. Root extracts shown to be effective only against B. subtilis. Fruit extracts showed the maximum antimicrobial activity against all the microbial species considered for the current study except against S. aureus. Highlight of the present study was the antimicrobial activity of callus extracts. DOI: http://dx.doi.org/10.3126/ijasbt.v2i4.11362 Int J Appl Sci Biotechnol, Vol. 2(4): 521-524

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Green synthesis and antimicrobial activity of ZnO nanostructures Punica granatum shell extract

Green Processing and Synthesis ◽

10.1515/gps-2016-0190 ◽

2017 ◽

Vol 6 (3) ◽

Cited By ~ 2

Author(s):

Ercan Karaköse ◽

Hakan Çolak ◽

Fatih Duman

Keyword(s):

Antimicrobial Activity ◽

Zinc Oxide ◽

Green Synthesis ◽

Aqueous Extract ◽

New Technologies ◽

Punica Granatum ◽

Zno Nanostructures ◽

Zno Nps ◽

Potential Applications ◽

Environmentally Safe

AbstractThe manufacture of nanoparticles (NPs) is a new area of investigation due to potential applications related to the improvement of new technologies; in particular, environmentally safe manufactured nanomaterials have become a growing area within nanoscience. In this research, we synthesized zinc oxide (ZnO)-NPs using an aqueous extract of

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More than Enzymes That Make or Break Cyclic Di-GMP—Local Signaling in the Interactome of GGDEF/EAL Domain Proteins of Escherichia coli

mBio ◽

10.1128/mbio.01639-17 ◽

2017 ◽

Vol 8 (5) ◽

Cited By ~ 60

Author(s):

Olga Sarenko ◽

Gisela Klauck ◽

Franziska M. Wilke ◽

Vanessa Pfiffer ◽

Anja M. Richter ◽

...

Keyword(s):

Escherichia Coli ◽

Biofilm Formation ◽

Second Messenger ◽

Bacterial Biofilm ◽

Interaction Patterns ◽

Systematic Analysis ◽

E Coli ◽

Diguanylate Cyclases ◽

Hub Proteins ◽

Effector Target

ABSTRACT The bacterial second messenger bis-(3′-5′)-cyclic diguanosine monophosphate (c-di-GMP) ubiquitously promotes bacterial biofilm formation. Intracellular pools of c-di-GMP seem to be dynamically negotiated by diguanylate cyclases (DGCs, with GGDEF domains) and specific phosphodiesterases (PDEs, with EAL or HD-GYP domains). Most bacterial species possess multiple DGCs and PDEs, often with surprisingly distinct and specific output functions. One explanation for such specificity is “local” c-di-GMP signaling, which is believed to involve direct interactions between specific DGC/PDE pairs and c-di-GMP-binding effector/target systems. Here we present a systematic analysis of direct protein interactions among all 29 GGDEF/EAL domain proteins of Escherichia coli . Since the effects of interactions depend on coexpression and stoichiometries, cellular levels of all GGDEF/EAL domain proteins were also quantified and found to vary dynamically along the growth cycle. Instead of detecting specific pairs of interacting DGCs and PDEs, we discovered a tightly interconnected protein network of a specific subset or “supermodule” of DGCs and PDEs with a coregulated core of five hyperconnected hub proteins. These include the DGC/PDE proteins representing the c-di-GMP switch that turns on biofilm matrix production in E. coli . Mutants lacking these core hub proteins show drastic biofilm-related phenotypes but no changes in cellular c-di-GMP levels. Overall, our results provide the basis for a novel model of local c-di-GMP signaling in which a single strongly expressed master PDE, PdeH, dynamically eradicates global effects of several DGCs by strongly draining the global c-di-GMP pool and thereby restricting these DGCs to serving as local c-di-GMP sources that activate specific colocalized effector/target systems. IMPORTANCE c-di-GMP signaling in bacteria is believed to occur via changes in cellular c-di-GMP levels controlled by antagonistic and potentially interacting pairs of diguanylate cyclases (DGCs) and c-di-GMP phosphodiesterases (PDEs). Our systematic analysis of protein-protein interaction patterns of all 29 GGDEF/EAL domain proteins of E. coli , together with our measurements of cellular c-di-GMP levels, challenges both aspects of this current concept. Knocking out distinct DGCs and PDEs has drastic effects on E. coli biofilm formation without changing the cellular c-di-GMP level. In addition, rather than generally coming in interacting DGC/PDE pairs, a subset of DGCs and PDEs operates as central interaction hubs in a larger "supermodule," with other DGCs and PDEs behaving as “lonely players” without contacts to other c-di-GMP-related enzymes. On the basis of these data, we propose a novel concept of “local” c-di-GMP signaling in bacteria with multiple enzymes that make or break the second messenger c-di-GMP.

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Chaperones mainly suppress primary nucleation during formation of functional amyloid required for bacterial biofilm formation

Chemical Science ◽

10.1039/d1sc05790a ◽

2022 ◽

Author(s):

Madhu Nagaraj ◽

Zahra Najarzadeh ◽

Jonathan Pansieri ◽

Ludmilla A. Morozova-Roche ◽

Henrik Biverstål ◽

...

Keyword(s):

Neurodegenerative Diseases ◽

Biofilm Formation ◽

Bacterial Biofilm ◽

Functional Amyloid ◽

E Coli ◽

Primary Nucleation ◽

Functional Amyloids

Unlike misfolding in neurodegenerative diseases, aggregation of functional amyloids involved in bacterial biofilm, e.g. CsgA (E. coli) and FapC (Pseudomonas), is carefully regulated. However, it is unclear whether functional aggregation...

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