scholarly journals Classification of Vertebral Osteomyelitis and Associated Judgment Applied during Post-Mortem Inspection of Swine Carcasses in Portugal

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1502
Author(s):  
Madalena Vieira-Pinto ◽  
Joana Azevedo ◽  
Patrícia Poeta ◽  
Isabel Pires ◽  
Lüppo Ellebroek ◽  
...  
Keyword(s):  
Classification Scheme ◽  
Vertebral Osteomyelitis ◽  
Meat Inspection ◽  
Economic Losses ◽  
Human Consumption ◽  
Histopathological Analysis ◽  
Microbiological Analysis ◽  
Finishing Pigs ◽  
The Eu

Vertebral osteomyelitis (VO) it is often a suppurative lesion that, in Portugal, represents the main cause of total condemnation of slaughtered finishing pigs. Based on the EU Meat Inspection legislation, meat from generalized VO cases presenting signs of pyemia should be declared unfit for human consumption. For that reason, the main objective of this study is to establish a classification scheme to differentiate between localized and generalized VO cases using macroscopic findings and validate it based on the presence of pyemia. To assist in, a combination of macroscopic characteristics of gross lesions (e.g., presence of pyaemia-related lesions (PRL), acute/chronic characteristics of VO) was used to create a classification scheme to differentiate between localized and generalized VO cases. The scheme was applied to 40 VO cases that had been totally condemned in an undifferentiated way. In those 40 cases, histopathological analysis was used to validate acute/chronic macro-criteria, and microbiological analysis was performed to identify the pyemia cases. From the 40 selected VO cases, 20 were macroscopically classified as chronic and 20 as acute. Cohen’s kappa coefficient (κ = 0.80; p < 0.001), revealed a substantial agreement between macroscopic and histopathology classification. Microbiological analyses identified 13 pyemia cases (13/40; 32.5%). Among those, 12 were macroscopically classified as acute, this association being highly significant (p < 0.001). By using the proposed VO classification scheme, 14 possible cases out of 40 could have been spared from total condemnation. This scheme can be used to harmonize the classification of VO and meat inspection decisions in Portuguese abattoirs. The output would lead to avoidance of unnecessary carcasses condemnation (food waste/economic losses), under an evidence-based approach, without compromising food safety and public health as demanded by the EU Meat Inspection legislation.

scholarly journals Veterinary meat inspection of bovine carcasses in theCzechRepublic during the period of 1995–2002

2012 ◽  
Vol 48 (No. 7) ◽  
pp. 183-189 ◽  
Author(s):  
V. Vecerek ◽  
A. Kozak ◽  
M. Malena ◽  
B. Tremlova ◽  
P. Chloupek
Keyword(s):  
Infectious Diseases ◽  
Respiratory Infections ◽  
Meat Inspection ◽  
Human Consumption ◽  
Safety Hazards ◽  
Food Borne ◽  
Cattle Herds

The results of veterinary meat inspection classification of 4&nbsp;000&nbsp;372 bovine carcasses reflect long-term aspects of health status in cattle herds and the quality of transport and handling of animals at slaughterhouses. Veterinary inspectors recorded the data obtained from meat inspection classification of bovine carcasses at slaughterhouses in the CzechRepublicduring the period of 1995&ndash;2002 together with the reasons for classification. The trends were evaluated by a comparison of two periods (Period I, 1995&ndash;1998, and Period II, 1999&ndash;2002) by means of calculating the indexes of values from Period II compared to those of Period I. Bovine carcasses classified as capable for human consumption (edible) were found in 87.87% of cases (88.83% during Period I and 86.58% during Period&nbsp;II, index 0.97), while those classified as capable for processing (conditionally edible) were found in 7.53% of cases (7.38% during Period I and 7.71% during Period II, index 1.04), and those condemned in 4.60% of cases (3.79% during Period I and 5.71% during Period II, index 1.51). The most important reason for classifying the carcasses as condemned was the finding of sensorial changes in meat, which occurred in 2.56% of cases (2.23% during Period I and 3.00% during Period II, index 1.35), followed by lesions due to non-infectious diseases &ndash; 1.00% (0.81% during Period I and 1.25% during Period II, index 1.53), added deleterious substances &ndash; 0.88% (0.60% during Period I and 1.27% during Period II, index 2.11), lesions due to respiratory infections &ndash; 0.03% (0.02% during Period I and 0.04% during Period II, index 1.74), and lesions due to miscellaneous infectious diseases &ndash; 0.10% (0.10% during Period I and 0.10% during Period II, index 1.05). Other reasons to condemn the carcasses included improper identification, lesions due to digestive infections, lesions due to tuberculosis, lesions due to paratuberculosis, lesions due to salmonellosis, leucosis and parasitic diseases. The occurrence of these conditions was on the level of mere hundredths of per cent. According to the results of meat inspection classification, the risk of food-borne diseases originating from bovine carcasses tends to be greater in the lesions due to non-infectious conditions with a long-term increasing trend. A&nbsp;considerable increase in the numbers of bovine carcasses condemned because of lesions due to paratuberculosis (index 4.62) represents an alarming finding with regard to potential food safety hazards.

scholarly journals Occurrence of Bovine Cysticercosis in Two Regions of the State of Tocantins-Brazil and the Importance of Pathogen Identification

Pathogens ◽  
2019 ◽  
Vol 8 (2) ◽  
pp. 66 ◽  
Author(s):  
Benta Natânia Silva FIGUEIREDO ◽  
Ricardo Alencar LIBóRIO ◽  
Megumi SATO ◽  
Camila Figueira da SILVA ◽  
Ronaldo Alves PEREIRA-JUNIOR ◽  
...  
Keyword(s):  
Meat Inspection ◽  
Diagnostic Methods ◽  
Economic Losses ◽  
Public Health Issue ◽  
Meat Production ◽  
Histopathological Analysis ◽  
Production Chain ◽  
Specific Pcr ◽  
Bovine Cysticercosis ◽  
Species Specific

Bovine cysticercosis, caused by Taenia saginata metacestodes, is the cause of significant economic losses to the meat production chain by condemnation and downgrading of infected carcasses. It is also a public health issue causing human taeniasis. This study evaluated the occurrence of bovine cysticercosis at the meat inspection procedures in slaughterhouses of south and north regions of the Tocantins State in Brazil. Specimens identified as cysts of T. saginata were collected and analyzed by molecular (PCR) and histopathological techniques. The cysts were collected from March to December of 2010 in slaughterhouses located in the cities of Alvorada (South) and Araguaína (North). The frequency of cystic lesions during the study was 0.033% (53/164,091) with 69.81% of calcified lesions and 30.9% of live cysts at meat inspection. From 14 samples submitted to molecular analysis, 28.57% (4/14) were positive for T. saginata. The histopathological analysis of the non-T. saginata samples showed lesions suggestive of granuloma and hydatid disease. The results indicated that the identification of the etiological agent is difficult by macroscopic inspection, emphasizing the need to associate specific diagnostic methods at meat inspection in abattoirs. In addition, species-specific PCR would be an effective tool for diagnosis, monitoring, and identifying cysticercosis, assisting the conventional tests.

Trimming and production losses associated with bacterial arthritis in lambs presented to an abattoir in southern Australia

2019 ◽  
Vol 59 (5) ◽  
pp. 933 ◽  
Author(s):  
Joan Lloyd ◽  
Johann Schröder ◽  
David Rutley
Keyword(s):  
Meat Products ◽  
Economic Cost ◽  
Meat Inspection ◽  
Carcass Weight ◽  
Economic Losses ◽  
Human Consumption ◽  
Bacterial Arthritis ◽  
Climatic Zones ◽  
Australian Standard ◽  
Production Losses

Bacterial arthritis in sheep is a painful and debilitating condition that is widespread across all sheep-raising regions and climatic zones of Australia. At slaughter, bacterial arthritis in sheep causes economic losses through the condemnation of all or parts of the carcass, as specified in the Australian Standard for the Hygienic Production and Transportation of Meat and Meat Products for Human Consumption. In this study, we investigated the economic cost of arthritis in lambs presenting to an abattoir in southern Australia using a combination of the prevalence of arthritis detected during meat inspection, condemnation rates, trim weight and carcass weight, and fat measurements. Data were collected on 354 lines of lambs representing 63287 carcasses. One hundred and sixty nine consignments, or approximately one-half of the consignments, had at least one carcass with arthritis/polyarthritis detected by meat inspection personnel. Four hundred and twenty-two, or 0.7%, of the carcasses had arthritis/polyarthritis in at least one joint. When arthritis was present, on average 2.0% of the line was affected. Three carcasses with arthritis were condemned and the remainder trimmed, with an average trim weight of 0.7 kg. In addition, arthritis reduced the growth of lambs by 1.2 kg hot standard carcass weight, approximately 2.7 kg liveweight (assuming 45% dressing percentage) and reduced fat cover by 1.8 mm.

scholarly journals Detection of Rhodococcus fascians, the Causative Agent of Lily Fasciation in South Korea

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 241
Author(s):  
Joon Moh Park ◽  
Jachoon Koo ◽  
Se Won Kang ◽  
Sung Hee Jo ◽  
Jeong Mee Park
Keyword(s):  
Phylogenetic Analysis ◽  
South Korea ◽  
Nicotiana Benthamiana ◽  
Economic Value ◽  
Virulence Gene ◽  
Economic Losses ◽  
Herbaceous Perennials ◽  
Rhodococcus Fascians ◽  
Different Strains

Rhodococcus fascians is an important pathogen that infects various herbaceous perennials and reduces their economic value. In this study, we examined R. fascians isolates carrying a virulence gene from symptomatic lily plants grown in South Korea. Phylogenetic analysis using the nucleotide sequences of 16S rRNA, vicA, and fasD led to the classification of the isolates into four different strains of R. fascians. Inoculation of Nicotiana benthamiana with these isolates slowed root growth and resulted in symptoms of leafy gall. These findings elucidate the diversification of domestic pathogenic R. fascians and may lead to an accurate causal diagnosis to help reduce economic losses in the bulb market.

scholarly journals Salmonella Infection in Nursery Piglets and Its Role in the Spread of Salmonellosis to Further Production Periods

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 123
Author(s):  
María Bernad-Roche ◽  
Alejandro Casanova-Higes ◽  
Clara M. Marín-Alcalá ◽  
Alberto Cebollada-Solanas ◽  
Raúl C. Mainar-Jaime
Keyword(s):  
Antimicrobial Resistance ◽  
Intestinal Content ◽  
Human Consumption ◽  
Finishing Pigs ◽  
Salmonella Infection ◽  
Mesenteric Lymph Nodes ◽  
Meat Juice ◽  
Infection Resistance ◽  
Common Serotypes ◽  
Breeding Farms

Few studies have focused on assessing Salmonella infection in the nursery and its role in further pig production periods. Mesenteric lymph nodes, intestinal content, and meat juice from 389 6-week-old male piglets intended for human consumption from five breeding farms and 191 pooled floor fecal samples from gilt development units (GDU) from the same farms were analyzed to estimate and characterize (by pulsed-field gel electrophoresis and antimicrobial resistance analyses) Salmonella infection. The prevalence of infection and shedding among piglets was 36.5% and 37.3%, respectively, shedding being significantly associated with infection (Odds Ratio = 12.7; CI 7.3–22.0). Salmonella Rissen; S. 4,[5],12:i:-; and S. Derby were the most common serotypes. A low level of Salmonella-specific maternal antibodies at the beginning of the nursery period suggested it was a period of high risk of infection. Resistance to 3rd- and 4th-generation cephalosporins was detected in piglet isolates although the piglets never received antibiotics, indicating they could be vectors of antimicrobial resistance. The same Salmonella clones were detected in piglet and GDU isolates, suggesting that infected piglets play a significant role in the infection of gilts and consequently of finishing pigs in the case of production farms. The control of Salmonella infection in nursery piglets may decrease the risk of abattoir and carcass contamination.

scholarly journals Is podocytopathy another image of renal affection in p-SLE?

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Hend H. Abdelnabi
Keyword(s):  
Lupus Erythematosus ◽  
Renal Involvement ◽  
Histopathological Analysis ◽  
Immune Deposits ◽  
Foot Process ◽  
Lupus Podocytopathy ◽  
Focal Segmental Sclerosis ◽  
Nephrotic Range Proteinuria

Abstract Background Lupus podocytopathy (LP) is a renal affection described in systemic lupus erythematosus (SLE) patients with nephrotic range proteinuria, characterized by diffuse foot process effacement without immune deposits and glomerular proliferation. This study describes LP, its pathological features and outcomes of pediatric (p-SLE) patients in comparison to the usual lupus nephritis (LN) cases. Methodology A retrospective cohort study conducted on a 10-year registration (2010–2019) of 140 p-SLE patients at the Pediatric Department, Tanta University. Histopathological analysis with light microscopy (LM) and immunofluorescence (IF) of all renal biopsies were evaluated according to the International Society of Nephrology Renal Pathology Society (ISN/RPS) grading system. In addition, some biopsies were examined with electron microscopy (EM). Results Eighty-six p-SLE cases (61.4%) had renal involvement; seventy-nine biopsies (91.86%) of them met the classification criteria of LN as defined by ISN/RPS system. Five biopsies were normal (MCD) and two showed focal segmental sclerosis (FSGN) that did not meet any known classification of LN. Hence, they were reevaluated using EM that revealed diffuse effaced podocytes without glomerular sub-epithelial, endocapillary or basement membrane immune deposits, and were classified as having lupus podocytopathy, representing (8.14%) of all LN biopsies. Those seven cases showed good response to steroids with a complete remission duration of 3.40 ± 1.95 weeks. However, some case had 1–3 relapses during the duration of follow up. Conclusions LP is a spectrum of p-SLE, not an association as it is related to disease activity and its initial presentation.

scholarly journals Experimental infection of broiler chicks with Salmonella Typhimurium from pigeon (Columba livia)

2016 ◽  
Vol 37 (4) ◽  
pp. 1919
Author(s):  
Átilla Holanda de Albuquerque ◽  
Régis Siqueira de Castro Teixeira ◽  
Débora Nishi Machado ◽  
Elisângela De Souza Lopes ◽  
Ruben Horn Vasconcelos ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Clinical Signs ◽  
Columba Livia ◽  
Poultry Feed ◽  
Economic Losses ◽  
Control Group ◽  
Broiler Chicks ◽  
Post Inoculation ◽  
Microbiological Analysis ◽  
Fecal Samples

Several cases of animal and human salmonellosis caused by the Salmonella serotype Typhimurium have been reported. In animals, subclinical infection favors pathogen dissemination through feces. In this context, the domestic pigeon (Columba livia) with an asymptomatic condition may play an important role in the transmission of salmonellosis, through the elimination of contaminated feces in commercial aviaries or in poultry feed facilities, causing economic losses to the poultry industry and presenting a risk to public health. This study aimed to evaluate the mortality, clinical signs and the presence of Salmonella Typhimurium in the feces and organs of chicks previously inoculated with bacteria isolated from a pigeon. One-day-old chicks were distributed in two experimental groups (G1 and G2) of 32 birds each, and a control group of six birds. Two inocula of 0.4 and 0.7 mL with 105 and 106 colony forming units were used in G1 and G2 birds, respectively. At 1, 4, 7 and 14 days post-inoculation (dpi) fecal samples were pooled from each cage and individual cloacal swabs were collected. At 14 dpi, all chicks were euthanized and samples were collected from the liver, spleen, lung, cecum and intestine for microbiological analysis. Mortality was only observed among G2 birds (6.25%). Most birds presented clinical signs of diarrhea at 4 dpi and no symptom as observed at 14 dpi. The results from cloacal swabs demonstrated bacterial elimination in 68.8% and 53.1% of G2 and G1 birds, respectively at 1 dpi. Additionally, fecal samples had elevated bacterial shedding in all four periods of observation , with a higher excretion at 4 dpi (62.5%) for both groups. Among G2 birds, 74.2% were positive for the pathogen in the intestine; G1 birds presented the lowest rate of lung infection (29%), and both groups had more than 50% positivity for liver and caeca. The results revealed that infected chicks with a Salmonella Typhimurium strains isolated from pigeons may host the pathogen in several organs, and simultaneously present diarrheic disorders with significant levels of bacterial excretion in feces.

scholarly journals The Use of UV Spectroscopy and SIMCA for the Authentication of Indonesian Honeys According to Botanical, Entomological and Geographical Origins

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 915
Author(s):  
Diding Suhandy ◽  
Meinilwita Yulia
Keyword(s):  
Spectral Data ◽  
Uv Spectroscopy ◽  
Moving Average ◽  
Food Product ◽  
Classification Method ◽  
Economic Losses ◽  
Food Fraud ◽  
Authentication System ◽  
Uv Visible

As a functional food, honey is a food product that is exposed to the risk of food fraud. To mitigate this, the establishment of an authentication system for honey is very important in order to protect both producers and consumers from possible economic losses. This research presents a simple analytical method for the authentication and classification of Indonesian honeys according to their botanical, entomological, and geographical origins using ultraviolet (UV) spectroscopy and SIMCA (soft independent modeling of class analogy). The spectral data of a total of 1040 samples, representing six types of Indonesian honey of different botanical, entomological, and geographical origins, were acquired using a benchtop UV-visible spectrometer (190–400 nm). Three different pre-processing algorithms were simultaneously evaluated; namely an 11-point moving average smoothing, mean normalization, and Savitzky–Golay first derivative with 11 points and second-order polynomial fitting (ordo 2), in order to improve the original spectral data. Chemometrics methods, including exploratory analysis of PCA and SIMCA classification method, was used to classify the honey samples. A clear separation of the six different Indonesian honeys, based on botanical, entomological, and geographical origins, was obtained using PCA calculated from pre-processed spectra from 250–400 nm. The SIMCA classification method provided satisfactory results in classifying honey samples according to their botanical, entomological, and geographical origins and achieved 100% accuracy, sensitivity, and specificity. Several wavelengths were identified (266, 270, 280, 290, 300, 335, and 360 nm) as the most sensitive for discriminating between the different Indonesian honey samples.

Geometric Characteristics of the Center-Point Curve Based on the Kinematics of the Compatibility Linkage

1992 ◽  
Author(s):  
J. A. Schaaf ◽  
J. A. Lammers
Keyword(s):  
Complex Number ◽  
Change Point ◽  
Double Point ◽  
Classification Scheme ◽  
Center Point ◽  
Degenerate Form ◽  
Point Curve ◽  
Position Synthesis ◽  
Point Form

Abstract In this paper we develop a method of characterizing the center-point curves for planar four-position synthesis. We predict the five characteristic shapes of the center-point curve using the kinematic classification of the compatibility linkage obtained from a complex number formulation for planar four-position synthesis. This classification scheme is more extensive than the conventional Grashof and non-Grashof classifications in that the separate classes of change point compatibility linkages are also included. A non-Grashof compatibility linkage generates a unicursal form of the center-point curve; a Grashof compatibility linkage generates a bicursal form; a single change point compatibility linkage generates a double point form; and a double or triple change point compatibility linkage generates a circular-degenerate or a hyperbolic-degenerate form.

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