scholarly journals Serological and Molecular Detection of Bovine Brucellosis at Institutional Livestock Farms in Punjab, Pakistan

2020 ◽  
Vol 17 (4) ◽  
pp. 1412 ◽  
Author(s):  
Tariq Jamil ◽  
Falk Melzer ◽  
Muhammad Saqib ◽  
Asim Shahzad ◽  
Khushal Khan Kasi ◽  
...  
Keyword(s):  
Odds Ratio ◽  
Multivariable Analysis ◽  
Cattle Breed ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Brucellosis ◽  
History Of ◽  
Polymerase Chain ◽  
Livestock Farms ◽  
Etiological Agents ◽  
First Time

Bovine brucellosis remains a persistent infection in ruminants in Pakistan. A total of 828 (409 buffaloes and 419 cattle) sera were collected from 11 institutional-owned livestock farms in Punjab, Pakistan. The samples were tested by rose bengal plate agglutination test (RBPT) and indirect enzyme-linked immunosorbent assay (iELISA). The seroprevalence along with 95% confidence interval (CI) was determined. Univariable and multivariable analysis of the epidemiological background data was conducted and odds ratio (OR) was calculated to understand any association between the risk factors and the seroprevalence. An overall seroprevalence of 3.9% (Positive/Tested = 32/828) and 3.3% (27/828) was detected by RBPT and iELISA, respectively. The seroprevalence of 5.6% (CI 3.6–8.3) and 4.7%, (CI 2.8–7.2) and the odds ratio of 2.63 (CI 1.20–5.77) and 2.50 (CI 1.08–5.78) for testing positive by RBPT and iELISA, respectively were significantly higher (p < 0.05) in buffaloes than in cattle. Breed, sex, history of abortion and retention of fetal membranes (RFM) in the animals were not found statistically significantly associated with the infection. RBPT and iELISA based results agreed almost perfect (k = 0.877). In total, Brucella abortus-DNA (9/27) was amplified from seropositive samples by real-time polymerase chain reaction. This study identified for the first time the etiological agents of brucellosis at a molecular level at institutional-owned livestock farms in Pakistan.

DETECTION OF SPECIFIC ANTIBODIES TO THE NUCLEOCAPSID PROTEIN FRAGMENTS OF SEVERE ACUTE RESPIRATORY SYNDROME-CORONAVIRUS AND SCOTOPHILUS BAT CORONAVIRUS-512 IN THREE INSECTIVOROUS BAT SPECIES

2018 ◽  
Vol 44 (04) ◽  
pp. 179-188 ◽  
Author(s):  
Yi-Ning Chen ◽  
Bo-Gang Su ◽  
Hung-Chang Chen ◽  
Cheng-Han Chou ◽  
Hsi-Chi Cheng
Keyword(s):  
Severe Acute Respiratory Syndrome ◽  
Nucleocapsid Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Protein Fragments ◽  
Specific Antibodies ◽  
History Of ◽  
Polymerase Chain ◽  
Carboxyl Terminal ◽  
Bat Coronavirus

Bats are the natural reservoirs of severe acute respiratory syndrome-coronavirus (SARS-CoV). Six Alphacoronavirus and five Betacoronavirus have been detected in many bat species, including SARS-related CoV and Middle East respiratory syndrome (MERS)-related CoV. In Taiwan, SARS-related CoV, belonging to Betacoronavirus, has been detected in Rhinolophus monoceros. Scotophilus bat CoV-512, belonging to Alphacoronavirus, has been detected in Scotophilus kuhlii, Miniopterus fuliginosus, and Rhinolophus monoceros by using reverse transcription polymerase chain reaction (RT-PCR). To understand the infection history of CoV in these three insectivorous bat populations, CoV-specific antibodies were surveyed by using western blot (WB) analysis and indirect enzyme-linked immunosorbent assay (ELISA). The carboxyl terminal fragment of nucleocapsid protein (N3) of SARS-CoV and Scotophilus bat CoV-512 were used as the antigen in the assays. Of the 52 serum samples obtained from Scotophilus kuhlii, 29 samples (56%) were tested positive for Scotophilus bat CoV-512-specific antibodies through ELISA. Of the 63 serum samples obtained from Rhinolophus monoceros, 9 samples were tested positive for only SARS-CoV-specific antibodies, 7 samples were tested positive for only Scotophilus bat CoV-512-specific antibodies, and 16 samples (25.4%) were tested positive for both antibodies through WB analysis. Only 1 of 18 Miniopterus bat serum samples tested positive for Scotophilus bat CoV-512-specific antibodies through ELISA. Lactating female bats had higher positive rates of CoV-specific antibodies than non-lactating female and male bats did. Our findings were crucial for understanding CoV infection history in three insectivorous bat species and important for the control of bat-borne zoonosis diseases.

Serum based polymerase chain reaction and enzyme linked immunosorbent assays for diagnosis of bovine brucellosis

2018 ◽  
Author(s):  
V. Naveen Kumar ◽  
M. Vijaya Bharathi ◽  
G. Selvaraju ◽  
K. Porteen ◽  
K. Vijayarani
Keyword(s):  
Polymerase Chain Reaction ◽  
Clinical Specimen ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Brucellosis ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Molecular Assays ◽  
Specific Pcr ◽  
Polymerase Chain ◽  
Sera Samples

Brucellosis is one of the economically important diseases in India and diagnosis of brucellosis using single test is cumbersome due to variation in sensitivity and specificity among the different test. The present study was aimed to assess the suitability of serum as clinical specimen in molecular diagnosis and evaluate the serology and molecular assays as in diagnosis of bovine brucellosis. A total of 821 bovine sera samples were subjected to indirect Enzyme Linked Immunosorbent Assay (i-ELISA) and serum based Polymerase Chain Reaction (PCR) assay. On serology 6.70 per cent positivity of brucellosis were reported and on PCR assay, 47 and 29 sera samples were positive for bcsp 31 genus specific and IS711 species specific PCR assay respectively with per cent positivity of 5.72 and 3.53. In comparison between serology and molecular test, 44 samples were positive for both assays and 11 and 3 samples were positive for serology and molecular assays individually. This study suggests that serum sample can be utilised as the choice of clinical specimen for both PCR assay and i-ELISA will be a future choice for the diagnosis of bovine brucellosis.

scholarly journals Seroprevalencia de brucelosis bovina y su relación con el aborto, en edad reproductiva en el cantón El Carmen, provincia Manabí, Ecuador.

2019 ◽  
pp. 87
Author(s):  
Juan Carlos Calderón Menéndez ◽  
Carlos Aníbal Bulnes Goicochea ◽  
Marina Dalila Zambrano Aguayo ◽  
María Hipatia Delgado Demera ◽  
Laura Monserrate De La Cruz Veliz ◽  
...  
Keyword(s):  
Reproductive Age ◽  
Confirmatory Test ◽  
Bovine Brucellosis ◽  
Rapid Technique ◽  
Test Elisa ◽  
Palabras Clave ◽  
History Of ◽  
Livestock Farms ◽  
Cattle Farms ◽  
The Relationship

Seroprevalence of bovine brucellosis and its relationship with abortion, in reproductive age in the canton of El Carmen, Manabí province, Ecuador Resumen                         El objetivo del presente estudio fue determinar la seroprevalencia de brucelosis bovina y su relación con el aborto en edad reproductiva en el cantón El Carmen, provincia Manabí, Ecuador, el cual se trabajó con 183 vacas en edad reproductiva en 20 ganaderías en el cantón El Carmen, provincia Manabí,  El Diagnóstico se realizó mediante la técnica rápida de Rosa de Bengala, y como prueba confirmatoria Elisa Competitiva, para hacer la relación entre la edad de las hembras y el historial de abortos se establecieron tres grupos: de 24 a 48, de 49 a 72 y mayores a 73 meses, considerando intervalos de 24 meses, como resultado se encontró una seroprevalencia de 5,46% de hembras en edad reproductiva, se estableció que el 20% de las fincas ganaderas estaban afectadas por la enfermedad en el cantón estudiado, se determinó que un 80% de las hembras enfermos presentaban antecedentes de abortos y se pudo apreciar con exactitud que las hembras con edades comprendidas entre los 49 a 72 meses presentaban el mayor porcentaje de abortos. Se concluye que el Cantón El Carmen presenta una seroprevalencia de 5,46%, que el 20% de las fincas ganaderas estaban afectadas por la enfermedad, un 80% de las hembras enfermos presentaban antecedentes de abortos, se determinó que las hembras con edades comprendidas entre los 49 a 72 meses presentaban el mayor porcentaje de abortos, en el cantón El Carmen Provincia Manabí, Ecuador. Palabras clave: Seroprevalencia; brucelosis; aborto; edad reproductiva; El Carmen. Abstract The objective of the present study was to determine the seroprevalence of bovine brucellosis and its relationship with abortion in reproductive age in the canton El Carmen, Manabí province, Ecuador, which worked with 183 cows of reproductive age in 20 farms in the canton of El Carmen , Manabí province, The diagnosis was made using the rapid technique of Rosa de Bengala, and as confirmatory test Elisa Competitiva, to make the relationship between the age of the females and the history of abortions were established three groups: from 24 to 48, 49 to 72 and greater than 73 months, considering intervals of 24 months, as a result a seroprevalence of 5.46% of females of reproductive age was found, it was established that 20% of the cattle farms were affected by the disease in the canton studied, it was determined that 80% of the sick females had antecedents of abortions and it was possible to appreciate with accuracy that the females with ages between 49 and 72 months had the highest percentage of abortions. It is concluded that Canton El Carmen presents a seroprevalence of 5.46%, that 20% of livestock farms were affected by the disease, 80% of sick females had antecedents of abortions, it was determined that females aged between 49 and 72 months they had the highest percentage of abortions, in the canton of El Carmen, Province of Manabi, Ecuador.Keywords: Seroprevalence; brucellosis; abortion; reproductive age; The Carmen Keywords: seroprevalence; brucellosis; abortion; reproductive age; El Carmen.

scholarly journals A novel adipose hormone asprosin as a potential breast cancer marker

2021 ◽  
Author(s):  
Sibel OZCAN ◽  
Nilgun YILDIRIM ◽  
Mesut YUR ◽  
Mehmet Ridvan OZDEDE ◽  
Mete OZCAN
Keyword(s):  
Breast Cancer ◽  
Odds Ratio ◽  
Roc Analysis ◽  
Gynecological Cancer ◽  
Multivariable Analysis ◽  
Serum Levels ◽  
Regression Analyses ◽  
Obese Adults ◽  
Potential Association ◽  
History Of

Abstract PurposeAsprosin is a recently discovered hormone released by white adipose tissue (WAT) that is typically significantly elevated in obese adults. Consequently, the adverse effects of increasing WAT in obesity during breast cancer (BC) development and progression have attracted interest of researchers and clinical practitioners. Thus, the aim of the present study was to determine whether the asprosin levels are associated with the probability of women having BC. MethodsThe study sample comprised of 45 female patients diagnosed with invasive BC and 42 healthy women that served as controls. Asprosin serum level was quantified in all subjects by ELISA, whereas serum levels of CEA and CA 15–3 were measured using an immunology analyzer. The potential association between asprosin and BC was examined through logistic regression analyses, while samples provided by BC patients were further subjected to ROC analysis to assess the diagnostic accuracy of asprosin. ResultsAsprosin levels were significantly higher in BC patients compared to healthy controls (2.38 ± 0.54 vs. 1.39 ± 0.53 ng/mL, p < 0.001). Multivariable analysis showed that the increased asprosin levels were associated with a significantly higher risk of breast cancer after adjustments for family history of breast and/or gynecological cancer, dyslipidemia, and BMI (odds ratio = 157.92; 95% confidence interval = 17.22−1447.96). When 1.78 ng/mL was adopted as the cut-off value, AUC, sensitivity, and specificity of asprosin for BC were 0.943, 91.1%, and 88.1%, respectively. ConclusionsOur findings demonstrate that asprosin is elevated in BC and can thus be an appropriate candidate for breast cancer diagnosis.

scholarly journals Seroprevalence of brucellosis in patients with prolonged fever in Bangladesh

2016 ◽  
Vol 10 (09) ◽  
pp. 939-946 ◽  
Author(s):  
AKM Anisur Rahman ◽  
Dirk Berkvens ◽  
Claude Saegerman ◽  
David Fretin ◽  
Noor Muhammad ◽  
...  
Keyword(s):  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Brucellosis ◽  
Chain Reaction ◽  
Serological Tests ◽  
Human Sera ◽  
Polymerase Chain ◽  
Species Specific ◽  
First Time ◽  
Sera Samples

Introduction: This study describes the seroprevalence of human brucellosis among pyretic patients and detection of Brucella abortus DNA from seropositive pyretic patients using real-time polymerase chain reaction (rtPCR) for the first time in Bangladesh. Methodology: Blood samples were collected from 300 pyretic patients from October 2007 to May 2008 and subjected to three serological tests: Rose-Bengal plate test (RBT), standard tube agglutination test (STAT), and indirect enzyme-linked immunosorbent assay (iELISA). Risk factors were identified by multivariate Firth’s logistic regression analysis. Brucella genus (BCSP31) and species-specific (IS711) rtPCR were applied to six human sera samples. Results: The seroprevalence of brucellosis among pyretic patients was estimated to be 2.0% (95% confidence interval [CI]: 0.74–4.30). The odds of brucellosis seropositivity were 8.9 (95% CI: 1.26–63.0) times higher in pyretic patients who handled goats than those who handled only cattle, whereas the odds of brucellosis seropositivity were 9.7 (95% CI: 1.28–73.68) times higher in pyretic patients who had backache compared to those without backache. B. abortus DNA was amplified from all six human sera that tested positive by RBT, STAT, and iELISA. As the agreement between the tests was very strong, RBT is recommended as a screening test for the diagnosis of human brucellosis in Bangladesh because it is easier to use, cheaper, and faster. Conclusions: Brucellosis among pyretic patients is common, and B. abortus is responsible for brucellosis in such patients. Pyretic patients who handle goats and those with backaches should be screened for brucellosis.

scholarly journals Contribution of Viral Respiratory Infections to Dengue-Like Illness Presentation at a Community Clinic in Southern Malaysia

2021 ◽  
Author(s):  
Amreeta Dhanoa ◽  
Chin Fang Ngim ◽  
Nor’azim Mohd Yunos ◽  
Syed M. Tupur Husain ◽  
Lian Yih Pong ◽  
...  
Keyword(s):  
Odds Ratio ◽  
Respiratory Infections ◽  
Respiratory Viruses ◽  
Multiple Logistic Regression ◽  
Chain Reaction ◽  
Community Clinic ◽  
History Of ◽  
Polymerase Chain ◽  
Viral Respiratory Infections ◽  
Viral Respiratory

This study explored the contribution of viral respiratory infections (VRIs) in dengue-like illness (DLI) patients and their distinguishing clinicolaboratory parameters. Two hundred DLI patients were prospectively recruited (1 July–1 October 2019) from a community clinic in Southern Malaysia. Patients ≥18 years with acute fever and fulfilling the WHO criteria of probable dengue were recruited. They underwent blood testing: blood counts, rapid dengue tests (nonstructural antigen-1/IgM) and polymerase chain reaction (PCR) for dengue, Zika, chikungunya, and Leptospira. Nasopharyngeal swabs (NPSs) were collected for FilmArray®RP2plus testing. From the 200 NPSs, 58 respiratory viruses (RVs) were detected in 54 patients. Of the 96 dengue-confirmed cases, 86 had dengue mono-infection, and 10 were coinfected with RVs. Of the 104 nondengue, 44 were RV positive and 4 Leptospira positive. Zika and chikungunya virus were not detected. Overall, the etiological diagnosis was confirmed for 72% of patients. Clinicolaboratory parameters were compared between dengue mono-infection and VRI mono-infection. Patients with coinfections were excluded. Multiple logistic regression showed that recent household/neighborhood history of dengue (adjusted odds ratio [aOR]: 5.9, 95% CI = 1.7–20.7), leukopenia (aOR: 12.5, 95% CI = 2.6–61.4) and thrombocytopenia (aOR: 5.5, 95% CI = 1.3–23.0) predicted dengue. Inversely, rhinorrhoea (aOR: 0.1, 95% CI = 0.01–0.3) and cough (aOR: 0.3, 95% CI = 0.1–0.9) favored VRI. Thus, VRIs comprise many infections diagnosed initially as DLIs. Early clinicolaboratory parameters can guide physicians screen patients for further testing.

scholarly journals A Common Polymorphism G-50T in Cytochrome P450 2J2 Gene Is Associated with Increased Risk of Essential Hypertension in a Russian Population

2008 ◽  
Vol 24 (2) ◽  
pp. 119-126 ◽  
Author(s):  
Alexey V. Polonikov ◽  
Vladimir P. Ivanov ◽  
Maria A. Solodilova ◽  
Irina V. Khoroshaya ◽  
Mikhail A. Kozhuhov ◽  
...  
Keyword(s):  
Essential Hypertension ◽  
Family History ◽  
Arachidonic Acid Metabolism ◽  
Russian Population ◽  
Common Polymorphism ◽  
Increased Risk ◽  
History Of ◽  
Family History Of Hypertension ◽  
Polymerase Chain ◽  
First Time

The present study was designed to test whether common polymorphism G-50T within the promoter of humanCYP2J2gene is associated with increased risk of essential hypertension in a Russian population. We studied 576 unrelated subjects, including 295 patients with hypertension and 281 healthy subjects. Genotyping for polymorphism G-50T of theCYP2J2gene was performed by polymerase chain reaction and restriction fragment length polymorphism techniques. The frequency of a −50T variant allele ofCYP2J2gene was significantly higher in patients with hypertension versus healthy controls (OR 4.03 95%CI 1.80–9.04p=0.0004). The association of a −50GT genotype with hypertension remained significant after adjustment for age, gender and family history of hypertension by multivariate logistic regression (OR 4.78 95%CI 1.87–12.27p=0.001). It has been found that OR for −50GT genotype × gender interaction (OR 4.48 95%CI 1.93–10.39p=0.00048) was slightly higher than OR for −50GT genotype (OR 4.43 95%CI 1.91–10.29p=0.00052), suggesting a weak effect of gender on the risk of hypertension in the heterozygous carriers of −50GT genotype. A family history of hypertension has no effect on the association between a −50GT genotype and hypertension. In present study we demonstrate for the first time that aCYP2J2*7 allele of theCYP2J2gene is clearly associated with an increased risk of essential hypertension. Furthermore, this study highlights the importance of P-450 epoxygenase pathway of arachidonic acid metabolism in the pathogenesis of hypertensive disease.

scholarly journals Seroprevalence and Associated Risk Factors of Bovine Brucellosis in District Gujranwala, Punjab, Pakistan

Animals ◽  
2021 ◽  
Vol 11 (6) ◽  
pp. 1744
Author(s):  
Muhammad Rashid Khan ◽  
Abdul Rehman ◽  
Salman Khalid ◽  
Mansur Ud Din Ahmad ◽  
Muhammad Avais ◽  
...  
Keyword(s):  
Risk Factors ◽  
Herd Size ◽  
Cross Sectional Study ◽  
Economic Losses ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bovine Brucellosis ◽  
Cross Sectional ◽  
History Of ◽  
Awareness Programs ◽  
Associated Risk Factors

Bovine brucellosis is a contagious zoonotic disease that causes economic losses through abortion and infertility. A cross-sectional study was designed to determine the seroprevalence and associated risk factors of bovine brucellosis in district Gujranwala of Punjab, Pakistan. A total of 220 bovine sera (112 from buffaloes, 108 from cattle) from 46 unvaccinated herds were collected. Parallel testing by the Rose Bengal Plate Test (RBPT) and Indirect Enzyme-linked Immunosorbent Assay (I-ELISA) showed a 58.7% (27/46) herd-level and 22.7% (50/220) animal-level seroprevalence. Seroprevalence was higher (p < 0.001, OR = 7.62) in adult animals (37.2%) compared to younger animals (4.9%). A herd size of >10 animals (p = 0.021, OR = 7.83), less housing space (p = 0.037, OR = 6.39) and history of abortion at the farm (p = 0.023, OR = 5.6) were found as risk factors associated with the seropositivity of brucellosis. There was a substantial agreement between the RBPT and I-ELISA results (Cohen’s kappa coefficient (κ) = 64.16, percent agreement = 89.5%). In conclusion, a relatively higher seroprevalence was found compared to the previous reports from the country. Standardization and validation of the advanced diagnostic tests would be needed. Biosecurity, personal protection, quarantine measures and routine screening of animals at the farm level and disease awareness programs and consumption of pasteurized milk in the human population will be helpful in preventing the transmission/zoonosis of the disease.

scholarly journals Unusual Presentation of Kawasaki Disease with Multisystem Inflammation and Antibodies Against Severe Acute Respiratory Syndrome Coronavirus 2: A Case Report

2020 ◽  
Author(s):  
Haena Kim ◽  
Jung Yeon Shim ◽  
Jae-Hoon Ko ◽  
Aram Yang ◽  
Jae Won Shim ◽  
...  
Keyword(s):  
Kawasaki Disease ◽  
Severe Acute Respiratory Syndrome ◽  
Signs And Symptoms ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibodies ◽  
Case Presentation ◽  
History Of ◽  
Polymerase Chain ◽  
High Index Of Suspicion ◽  
Inflammatory Syndrome

Abstract Background: Since mid-April 2020, cases of multisystem inflammatory syndrome in children (MIS-C) associated with coronavirus disease (COVID-19) that mimic Kawasaki disease (KD) have been reported in Europe and North America. However, no cases have been in East Asia, where KD is more prevalent.Case presentation: A previously healthy 11-year-old boy was admitted with a 4-day history of fever and abdominal pain. He had no contact history to any patient with COVID-19. Blood acute inflammatory markers were highly elevated. He was treated with antibiotics for suspected bacterial enteritis, but he suddenly developed hypotension. Inotropics and intravenous immunoglobulin were administered to manage septic shock. On hospitalization day 6, he developed signs and symptoms of KD (conjunctival injection, strawberry tongue, cracked lip, and coronary artery dilatation) in addition to pleural/pericardial effusion and mesenteric lymphadenitis. The results of microbiologic tests, including reverse-transcription polymerase chain reaction for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), were negative. Fluorescent immunoassay and enzyme-linked immunosorbent assay revealed abundant IgG antibodies against SARS-CoV-2 in his serum, but no IgM antibodies. He was discharged successfully on day 13.Conclusion: MIS-C may occur in children with a previously asymptomatic COVID-19 infection. A high index of suspicion is required for this novel syndrome in unusual cases of KD or KD shock syndrome with multisystem inflammation, even when there is no clear history of contact or symptoms of COVID-19.

scholarly journals Transplacental transmission of Anaplasma marginale in beef cattle chronically infected in southern Brazil

2013 ◽  
Vol 22 (2) ◽  
pp. 189-193 ◽  
Author(s):  
Hermann Eduardo Gonzalez Grau ◽  
Nilton Azevedo da Cunha Filho ◽  
Felipe Geraldo Pappen ◽  
Nara Amélia da Rosa Farias
Keyword(s):  
Transmission Rate ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Anaplasma Marginale ◽  
Enzyme Linked Immunosorbent Assay ◽  
Transplacental Transmission ◽  
Epidemiological Surveys ◽  
History Of ◽  
Newborn Calves ◽  
Polymerase Chain

In this study, we have investigated the incidence of transplacental transmission of Anaplasma marginale in chronically infected cows with no history of acute anaplasmosis during gestation. In addition, we evaluated various techniques for detection of transplacental transmission ofA. marginale. Blood samples were collected from 30 cows at three different periods: at the time of artificial insemination, at gestational diagnosis, and after calving. Also, blood was collected from the newborn calves, including one sample before colostrum intake, and another three days after birth. A. marginale-specific antibodies were detected in 100% of the cows with an indirect fluorescent antibody test (IFAT), and in 97% of them, using an indirect enzyme-linked immunosorbent assay (ELISA). Also, we observed that all of the three-day-old newborn calves were seropositive by IFAT. According to polymerase chain reaction, 63.3% of the cows were carriers of A. marginale, as well as 6.7% of the newborn calves. This represented a transplacental transmission rate of 10.5%. Furthermore, a correlation of 93.3% was observed between the two serodiagnostic techniques, demonstrating that both ELISA and IFAT can be used in epidemiological surveys of A. marginale. These results confirm the occurrence of transplacental transmission of A. marginale in chronically infected cows and suggest the importance of this transmission route in areas of enzootic instability.

Sign in / Sign up

Export Citation Format

Share Document