scholarly journals Cooperativity and Steep Voltage Dependence in a Bacterial Channel

2019 ◽  
Vol 20 (18) ◽  
pp. 4501
Author(s):  
Lin ◽  
Chang ◽  
Cherian ◽  
Wu ◽  
Phee ◽  
...  
Keyword(s):  
Voltage Dependence ◽  
Cooperative Behavior ◽  
Voltage Gating ◽  
The Novel ◽  
Membrane Channel ◽  
The Third ◽  
Novel Structure ◽  
Excitable Membranes ◽  
Direction Opposite ◽  
Channel Unit

This paper reports on the discovery of a novel three-membrane channel unit exhibiting very steep voltage dependence and strong cooperative behavior. It was reconstituted into planar phospholipid membranes formed by the monolayer method and studied under voltage-clamp conditions. The behavior of the novel channel-former, isolated from Escherichia coli, is consistent with a linearly organized three-channel unit displaying steep voltage-gating (a minimum of 14 charges in the voltage sensor) that rivals that of channels in mammalian excitable membranes. The channels also display strong cooperativity in that closure of the first channel permits the second to close and closure of the second channel permits closure of the third. All three have virtually the same conductance and selectivity, and yet the first and third close at positive potentials whereas the second closes at negative potentials. Thus, is it likely that the second channel-former is oriented in the membrane in a direction opposite to that of the other two. This novel structure is named “triplin.” The extraordinary behavior of triplin indicates that it must have important and as yet undefined physiological roles.

Feelings of Figures in Novel Notes Juang Fiersa Besari (Personality Ludwig Klages)

2020 ◽  
Vol 8 (3) ◽  
pp. 13
Author(s):  
Faridatus Soleha
Keyword(s):  
Qualitative Approach ◽  
Personality Structure ◽  
Personality Theory ◽  
The Novel ◽  
Research Focus ◽  
The Third ◽  
Analyze Data

This study aims to describe the feelings of the characters in the novel Juang Notes by Fiersa Bersari and researchers analyze using personality theory originated by Ludwig Klages by focusing his study on the personality structure of feelings. Feeling is a process of someone accepting or rejecting something in life. This study uses a qualitative approach by using library techniques to obtain data that will produce a description of the words or sentences in the observed study. In the analysis of this research using the hermeutics technique, in the hermeutics technique there are several stages, namely reading the research object in this study in the form of a fighting journal, the second gives a mark on the data that has been obtained from the reading results, the third provides code or coding on the data that has been found, and the fourth is to analyze data that has been obtained from the object of research in accordance with the specified research focus. Novel Notes Juang by Fiersa Besari is a novel that can be used as an inspiration for readers in living life. Based on the results of the study it was found that in the Fighting Notes novel there is a feeling that is divided into inner activities and the level of clarity, inner activities in the novel in the form of fear and guilt while viewed from the level of clarity in the form of happiness, sadness and longing.

The Coherence of Mary Barton: Romance, Realism, and Utopia

2015 ◽  
Vol 5 (3) ◽  
pp. 185-200
Author(s):  
Robert Z. Birdwell
Keyword(s):  
Thomas Carlyle ◽  
The Novel ◽  
Second Stage ◽  
The Third ◽  
Third Stage ◽  
Three Stages ◽  
Mode Of Production

Critics have argued that Elizabeth Gaskell's first novel, Mary Barton (1848), is split by a conflict between the modes of realism and romance. But the conflict does not render the novel incoherent, because Gaskell surpasses both modes through a utopian narrative that breaks with the conflict of form and gives coherence to the whole novel. Gaskell not only depicts what Thomas Carlyle called the ‘Condition of England’ in her work but also develops, through three stages, the utopia that will redeem this condition. The first stage is romantic nostalgia, a backward glance at Eden from the countryside surrounding Manchester. The second stage occurs in Manchester, as Gaskell mixes romance with a realistic mode, tracing a utopian drive toward death. The third stage is the utopian break with romantic and realistic accounts of the Condition of England and with the inadequate preceding conceptions of utopia. This third stage transforms narrative modes and figures a new mode of production.

Representasi Sikap Kebangsaan Anak Muda dalam Hegemoni Negara pada Novel Populer

Metahumaniora ◽  
2018 ◽  
Vol 8 (1) ◽  
pp. 100
Author(s):  
Muhamad Adji
Keyword(s):  
New Order ◽  
The Novel ◽  
The Third ◽  
Study Results ◽  
Popular Novel

AbstrakPenelitian ini bertujuan untuk memperoleh gambaran tentang sikap kebangsaananak muda Indonesia pada tiga novel populer yaitu Ali Topan Anak Jalanan karya TeguhEsha, Lupus: Makhluk Manis dalam Bis karya Hilman Hariwijaya, dan Balada Si Roy karya GolaGong. Alasan pemilihan objek penelitian di atas adalah karena ketiga novel tersebut memilikitingkat popularitas yang tinggi pada tiap zamannya sehingga membuat tokoh utamanyamenjadi representasi anak muda Indonesia pada zamannya masing-masing. Pertanyaanpertanyaanyang memandu tulisan ini adalah 1) Bagaimana keindonesiaan digambarkanpada ketiga novel tersebut dan 2) Bagaimana sikap kebangsaan direpresentasikan tokohanak muda pada ketiga novel tersebut. Dari hasil kajian didapatkan simpulan bahwasikap kebangsaan yang ditampilkan pada ketiga novel tersebut memiliki tingkatan yangberbeda-beda. Novel Ali Topan Anak Jalanan menunjukkan sikap kebangsaan dengan lebihkritis. Hegemoni orde Baru pada masa itu belum berhasil karena novel populer masihmenampilkan tokoh anak muda yang kritis dan cenderung memberontak terhadap nilainilaiideologi Orde Baru. Periode ’80-an menampilkan novel populer dengan tokoh anakmuda yang memiliki sikap kebangsaan yang terbelah. Pertama, anak muda yang dapatmenerima realitas yang dikontruksi Orde Baru, dengan menjalani sistem tersebut danmemposisikan dirinya sebagai bagian dari sistem tersebut. Hal itu direpresentasikan olehtokoh Lupus dalam novel Lupus: Makhluk Manis dalam Bis. Kedua, anak muda yang tidakdapat menerima realitas yang dikonstruksi Orde Baru, dengan cara keluar dari bagiansistem tersebut. Hal itu direpresentasikan oleh tokoh Roy dalam Balada Si Roy.Kata kunci: kebangsaan, anak muda, novel populer, Orde BaruAbstractThis study aims to obtain a picture of Indonesian nationalism in the three popularnovels that were Ali Topan Anak Jalanan by Teguh Esha, Lupus: Makhluk Manis dalam Bisby Hilman Hariwijaya, dan Balada Si Roy by Gola Gong. The reason for choosing the object ofresearch above is that all three novels are recognized to have high levels of popularity in eachera so that made the main characters became a representation of Indonesian youth at that time.The questions that guide the writing are 1) How Indonesianness described in the third novel?2) How does nationalism represented by youth in these three novels. From the study results isobtained the conclusion that nationalism is displayed on the third novel has a level different.Ali Topan Anak Jalanan show a nationalism more critically. The New Order’s hegemony atthat time has not been successful because the novel still showing a critical youth and tends torevolt against the ideological values of the New Order. The ‘80s period featured a popular novelwith a youth character who had a split nationalism. First, the youth who can accept the realitythat the New Order has constructed, with the system and position itself as part of the system.It was represented by the Lupus’s character in the Lupus: Makhluk Manis dalam Bis. Second,the youth who can not accept the facts constructed by the New Order, by going out from thatpart of the system. It was represented by Roy’s character in the Balada Si Roy.Keywords: nationalism, youth, popular novel, New Order

The Limit Situation And The Narrative Of War (On The Material Of The Novel “My Lieutenant” By Daniil Granin)

2020 ◽  
Vol 6 (1) ◽  
pp. 90-104
Author(s):  
Anatoly S. Kuprin ◽  
Galina I. Danilina
Keyword(s):  
20Th Century ◽  
Scientific Work ◽  
Point Of View ◽  
Continuous Limit ◽  
The Novel ◽  
Term Limit ◽  
Abstract Author ◽  
The Third ◽  
Definition Of

The purpose of this study is the analysis of limit situation in the narrative of war. The material of the study is the novel of Daniil Granin “My Lieutenant” and related texts. In the first part of the paper, the authors explore existing approaches to the term “limit situation” and similar concepts into scientific and philosophical traditions; limits of its applicability in literary studies and its relation to the categories of “narrative instances” and “event”. Proposed a literary-theoretical definition of the limit situation, which can be used in the analysis of fiction texts. Existing approaches to the examination of the situation of war are analyzed: philosophical-existential, psychoanalytic, sociological, literary. In the second part of the paper, the authors propose their method for analyzing limit situations in texts about war, which basis on existing approaches and preserves the text-centric principle of studying the structure of the story. Two interrelated areas of research have been identified: the study of war as a continuous limit situation in the intertextual aspect (the discourse of war); the study of limit situations (death, suffering, guilt, accident) in the narrative of war as part of a specific text. In the third part of the scientific work,the analysis of war as a continuous limit situation results in the study of the concept of “limit” (border) in a fiction text. The role of “limit” (border) concept in the texts about the war is studied, the possible types of limits in the discourse of war are examined. Limit situations in the narrative of war are analyzed on the basis of the novel “My Lieutenant” by Daniil Granin. A review of journalistic and scientific works about the novel revealed both the continuity and the differences between the novel and the “lieutenant” prose of the 20th century. An analysis of the limit situations in the novel revealed their key position in the narrative. These situations are independent of the fiction time, of the fluctuation of the point of view’; the function of the abstract author is to build the narrative as a “directive” immersion of the hero and narrator in these situations.

The Free Responsible Action of the Individual

2018 ◽  
Author(s):  
Michael P. DeJonge
Keyword(s):  
The Novel ◽  
Extreme Situation ◽  
The Third ◽  
Third Phase ◽  
Responsible Action ◽  
Action Type ◽  
Two Phases ◽  
The Individual ◽  
The Church

If, as Chapter 12 argues, much of Bonhoeffer’s resistance thinking remains stable even as he undertakes the novel conspiratorial resistance, what is new in his resistance thinking in the third phase? What receives new theological elaboration is the resistance activity of the individual, which in the first two phases was overshadowed by the resistance role played by the church. Indeed, as this chapter shows, Bonhoeffer’s conspiratorial activity is associated with what he calls free responsible action (type 6), and this is the action of the individual, not the church, in the exercise of vocation. As such, the conspiratorial activity is most closely related to the previously developed type 1 resistance, which includes individual vocational action in response to state injustice. But the conspiratorial activity differs from type 1 resistance as individual vocational action in the extreme situation.

Design and Analysis of a Novel Structure Form of Electro-Mechanical Transmission

2013 ◽  
Vol 694-697 ◽  
pp. 497-502
Author(s):  
Jiang Tao Gai ◽  
Shou Dao Huang ◽  
Guang Ming Zhou ◽  
Yi Yuan
Keyword(s):  
Mechanical Transmission ◽  
The Novel ◽  
Structure Form ◽  
Tracked Vehicle ◽  
Power Coupling ◽  
Advantages And Disadvantages ◽  
Vehicle Propulsion ◽  
Novel Structure ◽  
Straight Line ◽  
Planetary Mechanism

In order to search after a new way of the propulsion system of tracked vehicle, a novel structure form of electro-mechanical transmission was developed in this paper, through analyzing the advantages and disadvantages of existing projects of electric drive system for tracked vehicle. It could increase the rate of power exertion obviously and synthesize the mechanical and electrical strongpoint. And based on the structure form, an electro-mechanical transmission was designed with double electromotor added planetary mechanism of steering power coupling and gearshift, considering engineering realization. And then straight-line driving and steering performances of the transmission were calculated which proved that the novel electro-mechanical transmission could meet the requirement of tracked vehicle propulsion well.

scholarly journals A Novel 4H-SiC MESFET with a Heavily Doped Region, a Lightly Doped Region and an Insulated Region

Micromachines ◽  
2021 ◽  
Vol 12 (5) ◽  
pp. 488
Author(s):  
Hujun Jia ◽  
Mengyu Dong ◽  
Xiaowei Wang ◽  
Shunwei Zhu ◽  
Yintang Yang
Keyword(s):  
Breakdown Voltage ◽  
Direct Current ◽  
Oscillation Frequency ◽  
The Novel ◽  
Saturation Current ◽  
Excellent Performance ◽  
Maximum Oscillation Frequency ◽  
Power Added Efficiency ◽  
Novel Structure ◽  
Heavily Doped

A novel 4H-SiC MESFET was presented, and its direct current (DC), alternating current (AC) characteristics and power added efficiency (PAE) were studied. The novel structure improves the saturation current (Idsat) and transconductance (gm) by adding a heavily doped region, reduces the gate-source capacitance (Cgs) by adding a lightly doped region and improves the breakdown voltage (Vb) by embedding an insulated region (Si3N4). Compared to the double-recessed (DR) structure, the saturation current, the transconductance, the breakdown voltage, the maximum oscillation frequency (fmax), the maximum power added efficiency and the maximum theoretical output power density (Pmax) of the novel structure is increased by 24%, 21%, 9%, 11%, 14% and 34%, respectively. Therefore, the novel structure has excellent performance and has a broader application prospect than the double recessed structure.

Richardson's Pamela: An Interpretation

PMLA ◽  
1973 ◽  
Vol 88 (1) ◽  
pp. 79-91
Author(s):  
Stuart Wilson
Keyword(s):  
Moral Principle ◽  
The Novel ◽  
The Third ◽  
Narrative Process

The heroine of Richardson's first novel is neither a meretricious young hussy nor a paragon of virtue; she is a complex personality who moves from a naive adolescence to a composed maturity in the course of the narrative. The conflict between her devotion to moral principle and her growing affection for Mr. B., which develops in the first, or Bedfordshire, section of the novel, brings a near-psychic collapse in the second, or Lincolnshire, section. The imagery and symbolism show the nature of her torments, her growing awareness of a love that combines eros and agape, and her need for the reconciliation between conscience and libido which is completed after her return to the Bedfordshire estate in the third section. The formal symmetry of the novel evolves from the narrative process within which Pamela is tested and proved capable of an honest love and a tranquil marriage.

scholarly journals Identification of a translocated gating charge in a voltage-dependent channel. Colicin E1 channels in planar phospholipid bilayer membranes.

1991 ◽  
Vol 98 (1) ◽  
pp. 77-93 ◽  
Author(s):  
C K Abrams ◽  
K S Jakes ◽  
A Finkelstein ◽  
S L Slatin
Keyword(s):  
Voltage Dependence ◽  
Ion Selectivity ◽  
Lipid Vesicles ◽  
Voltage Gating ◽  
Site Directed Mutagenesis ◽  
Phospholipid Bilayer ◽  
Voltage Gated ◽  
Colicin E1 ◽  
Gating Charge ◽  
Ion Conducting

The availability of primary sequences for ion-conducting channels permits the development of testable models for mechanisms of voltage gating. Previous work on planar phospholipid bilayers and lipid vesicles indicates that voltage gating of colicin E1 channels involves translocation of peptide segments of the molecule into and across the membrane. Here we identify histidine residue 440 as a gating charge associated with this translocation. Using site-directed mutagenesis to convert the positively charged His440 to a neutral cysteine, we find that the voltage dependence for turn-off of channels formed by this mutant at position 440 is less steep than that for wild-type channels; the magnitude of the change in voltage dependence is consistent with residue 440 moving from the trans to the cis side of the membrane in association with channel closure. The effect of trans pH changes on the ion selectivity of channels formed by the carboxymethylated derivative of the cysteine 440 mutant independently establishes that in the open channel state, residue 440 lies on the trans side of the membrane. On the basis of these results, we propose that the voltage-gated opening of colicin E1 channels is accompanied by the insertion into the bilayer of a helical hairpin loop extending from residue 420 to residue 459, and that voltage-gated closing is associated with the extrusion of this loop from the interior of the bilayer back to the cis side.

scholarly journals NILAI TANGGUNG JAWAB DALAM NOVEL LASKAR PELANGI KARYA ANDREA HIRATA: TINJAUAN STRUKTURALISME GENETIK

2019 ◽  
Vol 1 (1) ◽  
pp. 43-56
Author(s):  
Siti Rukiyah
Keyword(s):  
World View ◽  
Cultural Background ◽  
Moral Values ◽  
The Novel ◽  
Self Confidence ◽  
Novel Structure ◽  
Livelihood Systems ◽  
Content Analysis Method ◽  
And Behavior ◽  
Attitudes And Behavior

This study discusses in depth about the moral values of responsibility in the novel Laskar Pelangi. Furthermore, the proposed related to the socio-cultural background of the authors in the creation of novel Laskar Pelangi effect on moral values. In addition, also discussed the author's view of the world related to the teachings of the value of responsibility. This research uses a qualitative approach with content analysis method. The results showed that the value of responsibility based on the novel structure consisting of man's relationship to himself, namely in terms of character and characterization. The characters are displayed Andrea Hirata bring the characters responsible for the thoughts, attitudes, and behavior. From a review of genetic structuralism, the value of responsibility based on social and cultural background, knowledge systems also influence the social and cultural life. Belitung people's livelihood systems are revealed also shape the character responsible. The author's world view of morals includes the value of self-existence, self-esteem, self-confidence, fear, longing, and responsibility.

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