scholarly journals Anti-Inflammatory Effect of Licochalcone A via Regulation of ORAI1 and K+ Channels in T-Lymphocytes

2021 ◽  
Vol 22 (19) ◽  
pp. 10847
Author(s):  
Hong T. L. Phan ◽  
Hyun J. Kim ◽  
Sungwoo Jo ◽  
Woo K. Kim ◽  
Wan Namkung ◽  
...  
Keyword(s):  
T Lymphocytes ◽  
Vital Role ◽  
K Channels ◽  
Licochalcone A ◽  
Beneficial Effects ◽  
Cellular Processes ◽  
Proliferation And Differentiation ◽  
Ic50 Values ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Calcium signaling plays a vital role in the regulation of various cellular processes, including activation, proliferation, and differentiation of T-lymphocytes, which is mediated by ORAI1 and potassium (K+) channels. These channels have also been identified as highly attractive therapeutic targets for immune-related diseases. Licochalcone A is a licorice-derived chalconoid known for its multifaceted beneficial effects in pharmacological treatments, including its anti-inflammatory, anti-asthmatic, antioxidant, antimicrobial, and antitumorigenic properties. However, its anti-inflammatory effects involving ion channels in lymphocytes remain unclear. Thus, the present study aimed to investigate whether licochalcone A inhibits ORAI1 and K+ channels in T-lymphocytes. Our results indicated that licochalcone A suppressed all three channels (ORAI1, Kv1.3, and KCa3.1) in a concentration-dependent matter, with IC50 values of 2.97 ± 1.217 µM, 0.83 ± 1.222 µM, and 11.21 ± 1.07 µM, respectively. Of note, licochalcone A exerted its suppressive effects on the IL-2 secretion and proliferation in CD3 and CD28 antibody-induced T-cells. These results indicate that the use of licochalcone A may provide an effective treatment strategy for inflammation-related immune diseases.

scholarly journals In vitro evidence for the involvement of H2S pathway in the effect of clodronate during inflammatory response

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Rosangela Montanaro ◽  
Alessio D’Addona ◽  
Andrea Izzo ◽  
Carlo Ruosi ◽  
Vincenzo Brancaleone
Keyword(s):  
Inflammatory Markers ◽  
In Vitro Study ◽  
Inos Expression ◽  
Beneficial Effects ◽  
Tnf Α ◽  
Inflammatory State ◽  
Anti Inflammatory ◽  
Underlying Mechanisms ◽  
Inflammatory Effect

AbstractClodronate is a bisphosphonate agent commonly used as anti-osteoporotic drug. Throughout its use, additional anti-inflammatory and analgesic properties have been reported, although the benefits described in the literature could not solely relate to their inhibition of bone resorption. Thus, the purpose of our in vitro study is to investigate whether there are underlying mechanisms explaining the anti-inflammatory effect of clodronate and possibly involving hydrogen sulphide (H2S). Immortalised fibroblast-like synoviocyte cells (K4IM) were cultured and treated with clodronate in presence of TNF-α. Clodronate significantly modulated iNOS expression elicited by TNF-α. Inflammatory markers induced by TNF-α, including IL-1, IL-6, MCP-1 and RANTES, were also suppressed following administration of clodronate. Furthermore, the reduction in enzymatic biosynthesis of CSE-derived H2S, together with the reduction in CSE expression associated with TNF-α treatment, was reverted by clodronate, thus rescuing endogenous H2S pathway activity. Clodronate displays antinflammatory properties through the modulation of H2S pathway and cytokines levels, thus assuring the control of the inflammatory state. Although further investigation is needed to stress out how clodronate exerts its control on H2S pathway, here we showed for the first the involvement of H2S in the additive beneficial effects observed following clodronate therapy.

scholarly journals The Beneficial Biological Properties of Salicylic Acid

2015 ◽  
Vol 32 (4) ◽  
pp. 259-265 ◽  
Author(s):  
Pavle Randjelović ◽  
Slavimir Veljković ◽  
Nenad Stojiljković ◽  
Dušan Sokolović ◽  
Ivan Ilić ◽  
...  
Keyword(s):  
Salicylic Acid ◽  
Well Being ◽  
Biological Properties ◽  
Vital Role ◽  
Prostaglandin Synthesis ◽  
Beneficial Effects ◽  
Inhibition Of Prostaglandin Synthesis ◽  
Anti Inflammatory ◽  
Antiinflammatory Effects

Summary Salicylic acid is a phytochemical with beneficial effects on human well-being. Salicylic acid is a phenolic compound and is present in various plants where it has a vital role in protection against pathogenic agents. Natural sources include fruits, vegetables and spices. The most famous and defined effect of salicylic acid is prostaglandin synthesis inhibition. Salicylic acid has antiinflammatory effects through suppression of transcription of genes for cyclooxygenase. Most of the pharmacological properties of salicylic acid can be contributed to the inhibition of prostaglandin synthesis. Also, it was discovered that salicylic acid has other in vivo cyclooxygenase-independent pathways. Since salicylic acid does not inhibit cyclooxygenase considerably, the anti-inflammatory effect is not a consequence of direct inhibition of cyclooxygenase activity. Because of its fundamental role, it was suggested that inhibition of nuclear factor kappa B by salicylic acid is one of the key anti-inflammatory mechanisms of action for salicylates. One of the most studied properties of salicylic acid is its antioxidative activity. Salicylic acid is a confirmed inhibitor of oxidative stress. Salicylic acid is capable of binding iron. This fact is significant for antioxidative effect of salicylic acid because iron has an important function in the course of lipid peroxidation.

scholarly journals A Review on the Anti-Inflammatory Activity of Pomegranate in the Gastrointestinal Tract

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Elisa Colombo ◽  
Enrico Sangiovanni ◽  
Mario Dell'Agli
Keyword(s):  
Gastrointestinal Tract ◽  
Clinical Studies ◽  
Intestinal Tract ◽  
Biological Activities ◽  
Inflammatory Activity ◽  
Beneficial Effects ◽  
Anti Inflammatory ◽  
Gastro Intestinal Tract ◽  
Inflammatory Effect

Several biological activities of pomegranate have been widely described in the literature, but the anti-inflammatory effect in the gastrointestinal tract has not been reviewed till now. The aim of the present paper is to summarize the evidence for or against the efficacy of pomegranate for coping with inflammatory conditions of the gastro-intestinal tract. The paper has been organized in three parts: (1) the first one is devoted to the modifications of pomegranate active compounds in the gastro-intestinal tract; (2) the second one considering the literature regarding the anti-inflammatory effect of pomegranate at gastric level; (3) the third part considers the anti-inflammatory effect of pomegranate in the gut.In vivostudies performed on the whole fruit or juice, peel, and flowers demonstrate antiulcer effect in a variety of animal models. Ellagic acid was the main responsible for this effect, although other individual ellagitannins could contribute to the biological activity of the mixture. Different preparations of pomegranate, including extracts from peels, flowers, seeds, and juice, show a significant anti-inflammatory activity in the gut. No clinical studies have been found, thus suggesting that future clinical studies are necessary to clarify the beneficial effects of pomegranate in the gastrointestinal tract.

scholarly journals Efficient and Divergent Enantioselective Syntheses of DHPVs and Anti-Inflammatory Effect on IEC-6 Cells

Molecules ◽  
2020 ◽  
Vol 25 (9) ◽  
pp. 2215
Author(s):  
Hyun Su Kim ◽  
Sungkyun Chung ◽  
Moon-Young Song ◽  
Changjin Lim ◽  
Hyeyoung Shin ◽  
...  
Keyword(s):  
Beneficial Effects ◽  
Asymmetric Dihydroxylation ◽  
Cross Metathesis ◽  
Molecular Therapeutics ◽  
Inflammatory Bowel ◽  
Anti Inflammatory ◽  
Enantioselective Syntheses ◽  
Precise Molecular Mechanism ◽  
Significant Attention ◽  
Inflammatory Effect

Despite numerous reports on the beneficial effects of catechin or epicatechin contained in tea and cacao extract on human health, a conclusive and precise molecular mechanism has not been elucidated. Metabolism of chemical compounds in gut microbiota recently gained significant attention, and extensive studies have been devoted in this field. In conjunction with these results, our group focused on the anti-inflammatory effects of both enantiomers of DHPV (5-(3′,4′-dihydroxyphenyl)-γ-valerolactone), produced in the intestine by microbiota metabolism, on IEC-6 cells. Divergent and efficient enantioselective synthesis of (S)- and (R)-DHPV was efficiently achieved by cross-metathesis and Sharpless asymmetric dihydroxylation as a key reaction for four steps in 16% and 14% overall yields, respectively. The anti-inflammatory effects of two enantiomers were tested on IEC-6 cells, and we found that (S)-DHPV was more active than (R)-DHPV. This result implicates that the metabolite produced in the gut has beneficial effects on IEC-6 cells of rat intestines, and the chirality of the metabolite is important for its anti-inflammatory activity. This also provided information for the future discovery of novel small molecular therapeutics for the treatment of inflammatory bowel disease.

scholarly journals Anti-Inflammatory, Antioxidant and Anti-Diarrheal Effects of Ethanol Extract of Stephania Japonica

1970 ◽  
Vol 46 (4) ◽  
pp. 437-442
Author(s):  
NU Ahmed ◽  
R Akter ◽  
Mohammed A Satter ◽  
MS Khan ◽  
F Islam ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Dosage Level ◽  
Scavenging Effect ◽  
Ic50 Values ◽  
Radical Scavenging Effect ◽  
Anti Inflammatory ◽  
Free Radical Scavenging Effect ◽  
Inflammatory Effect

The present study was carried out to investigate anti-inflammatory, antioxidant and anti-diarrheal effect of ethanol extract of Stephania japonica. This study showed that the plant extract has significant (p<0.05) anti-inflammatory effect at all phases of carrageenan induced inflammation at a dose level 2g/kg. The DPPH free radical scavenging effect of the extract was compared with standard antioxidant ascorbic acid. IC50 values were found 33.57 μg/ml for the extract and 15.57 μg/ml for ascorbic acid. S. japonica extract at dosage level 2g/kg and 1g/kg decreased the gastrointestinal motility 36.56 and 21.53 %, respectively, in rats. The ethanol extract of the plant also reduced the total number of feces as well as wet feces of rats in castor oil-induced diarrheal model. The results revealed that the extract possesses promising anti-inflammatory, antioxidant and antidiarrheal activity. Keywords: Anti-inflammatory; Antioxidant; Anti-diarrheal; Stephania japonica DOI: http://dx.doi.org/10.3329/bjsir.v46i4.9587 BJSIR 2011; 46(4): 437-442

Anti-Inflammatory Effect of the Kampo Japanese Traditional Medicine daikenchuto After Colorectal Resection

2015 ◽  
Vol 100 (11-12) ◽  
pp. 1360-1363
Author(s):  
Takaaki Fujii ◽  
Hiroki Morita ◽  
Toshinaga Sutoh ◽  
Reina Yajima ◽  
Soichi Tsutsumi ◽  
...  
Keyword(s):  
Colorectal Resection ◽  
Control Group ◽  
Impaired Liver Function ◽  
Elective Colorectal Surgery ◽  
Inflammatory Reactions ◽  
Beneficial Effects ◽  
Anti Inflammatory ◽  
Japanese Traditional Medicine ◽  
Wbc Count ◽  
Inflammatory Effect

Here we analyzed whether Daikenchuto (DKT) suppresses postoperative inflammatory reactions in patients who have undergone elective colorectal surgery. DKT is one of the most frequently prescribed traditional (Kampo) medicines in Japan. DKT is reported to have various beneficial effects on bowel disorders. It was recently reported that DKT also has an anti-inflammatory effect. Consecutive patients with colorectal cancer who underwent elective colorectal resection were separated into two groups. A total of 67 patients began a DKT regimen after surgery (DKT group) and 53 patients did not begin the DKT regimen after surgery (control group). We observed a steady postsurgery increase in the white blood cell (WBC) count of all patients on postoperative day 1 (POD 1), whereas the WBC count decreased at PODs 3 and 7. When we compared the WBC values of the DKT group (6147.2 ± 2217.3 per microliter) and control (7071.1 ± 2828.0 per microliter) groups on POD 7, we found significant differences (P = 0.038). There was no significant side effect due to DKT, except for one case of mildly impaired liver function. Our results suggest that DKT administration may have an anti-inflammatory effect during the postoperative period. Further studies are warranted to investigate the possibility of using DKT as a therapeutic agent based on its anti-inflammatory effect.

scholarly journals The Anti-Inflammatory Effect of Taurine on Cardiovascular Disease

Nutrients ◽  
2020 ◽  
Vol 12 (9) ◽  
pp. 2847 ◽  
Author(s):  
Tawar Qaradakhi ◽  
Laura Kate Gadanec ◽  
Kristen Renee McSweeney ◽  
Jemma Rose Abraham ◽  
Vasso Apostolopoulos ◽  
...  
Keyword(s):  
Sulfonic Acid ◽  
Renin Angiotensin System ◽  
Animal Tissues ◽  
Cellular Functions ◽  
Protein Amino Acid ◽  
Angiotensin System ◽  
Beneficial Effects ◽  
Ion Movement ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Taurine is a non-protein amino acid that is expressed in the majority of animal tissues. With its unique sulfonic acid makeup, taurine influences cellular functions, including osmoregulation, antioxidation, ion movement modulation, and conjugation of bile acids. Taurine exerts anti-inflammatory effects that improve diabetes and has shown benefits to the cardiovascular system, possibly by inhibition of the renin angiotensin system. The beneficial effects of taurine are reviewed.

Naringin and 5-fluorouracil suppress inflammatory Cytokines in human skin cancer cell line

2021 ◽  
Vol 12 (1) ◽  
pp. 729-733
Author(s):  
Deepa Suruli ◽  
Fathima Bushra Sheriff Mirza ◽  
Gloria Jemmi Christobel R ◽  
Amuthavalli Kottaiswamy ◽  
Shila Samuel ◽  
...  
Keyword(s):  
Skin Cancer ◽  
Cell Line ◽  
Human Skin ◽  
Cell Lines ◽  
Cancer Cell ◽  
A375 Cell ◽  
Ic50 Values ◽  
Anti Inflammatory ◽  
A375 Cells ◽  
Inflammatory Effect

Naringin is a citrus flavonoid recently studied for anti-inflammatory activity in numerous cancer cells. In this study, the anti-inflammatory properties of naringin along with 5-fluorouracil in human skin cancer cell lines A375 was analyzed. A375 cells were treated with naringin, 5-fluorouracil alone, and combination. MTT assay and cell viability assays was demonstrated to detect the inhibitory effects of naringin or 5-fluorouracil on cell proliferation. mRNA expression of TNFα, IL-6, IL-1β, and NFκB were determined using quantitative RT-PCR.  The effect of naringin and 5-fluorouracil combination significantly inhibited the growth and proliferation of the A375 cells in a concentration dependent manner with the IC50 values of naringin (24.75 μM) 5-fluorouracil (2.5 μM). The combination of naringin+5-fluorouracil on A375 cell lines at a concentration of half IC50 values (12µM+1 μM). Naringin and 5-fluorouracil combination also decreased the level of TNFα, IL-6, IL-1β, and NFκB mRNA in the A375 cell line. Naringin and 5-fluorouracil exerted anti-inflammatory effect through the suppression of NF-kB, IL-1β, TNFα, IL-6 in A375 cells. Taken together, our results suggested that treating A375 with naringin and 5-fluorouracil combination may have future applications in treating skin cancers through its anti-inflammatory effect.

scholarly journals Restoration of Chloride Efflux by Azithromycin in Airway Epithelial Cells of Cystic Fibrosis Patients

2011 ◽  
Vol 55 (4) ◽  
pp. 1792-1793 ◽  
Author(s):  
Vinciane Saint-Criq ◽  
Carine Rebeyrol ◽  
Manon Ruffin ◽  
Telma Roque ◽  
Loïc Guillot ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Airway Epithelial Cells ◽  
Chronic Obstructive ◽  
Airway Epithelial ◽  
Respiratory Condition ◽  
Beneficial Effects ◽  
Chronic Obstructive Pulmonary Diseases ◽  
Anti Inflammatory ◽  
Inflammatory Effect ◽  
Airway Cells

ABSTRACTAzithromycin (AZM) has shown promising anti-inflammatory properties in chronic obstructive pulmonary diseases, and clinical studies have presented an improvement in the respiratory condition of cystic fibrosis (CF) patients. The aim of this study was to investigate, in human airway cells, the mechanism by which AZM has beneficial effects in CF. We demonstrated that AZM did not have any anti-inflammatory effect on CF airway cells but restored Cl−efflux.

Anti-Inflammatory Effect of White Wine in CKD Patients and Healthy Volunteers

2015 ◽  
Vol 39 (1-3) ◽  
pp. 218-223 ◽  
Author(s):  
Massimiliano Migliori ◽  
Vincenzo Panichi ◽  
Rafael de la Torre ◽  
Montserrat Fitó ◽  
Maribel Covas ◽  
...  
Keyword(s):  
Healthy Volunteers ◽  
Olive Oil ◽  
Plasma Levels ◽  
Inflammatory Markers ◽  
Virgin Olive Oil ◽  
Extra Virgin Olive Oil ◽  
White Wine ◽  
Beneficial Effects ◽  
Anti Inflammatory ◽  
Inflammatory Effect

Background: Mediterranean-style diet has been considered for its important beneficial effects on the progression of CV disease. Wine is an important component of the Mediterranean diet, and moderate wine drinkers have lower mortality rates than nondrinkers and heavy drinkers in epidemiologic studies. The beneficial effects of red wine are thought to be dependent on the polyphenol compounds such as resveratrol that exhibit potent antioxidant activity. However, white wine, although lacking polyphenols, contains simple phenols, such as tyrosol (Tyr) and hydroxytyrosol (OH-Tyr), characteristic also of extra-virgin olive oil, which may share similar antioxidant and inflammatory properties. Patients and Methods: The effect of white wine and extra-virgin olive oil on inflammatory markers was evaluated in 10 healthy volunteers and in 10 patients with CKD (chronic kidney disease) K-DOQI stage III-IV in a prospective, single blind, randomized, cross-over trial. After two weeks of wash-out from alcoholic beverages, subjects were randomized to a cross-over design A-B or B-A of a 2-week treatment with white wine (4 ml/kg body weight, 0.48 g/kg of alcohol 12%, corresponding to 2-3 glasses/daily) and extra-virgin olive oil (treatment A) or extra-virgin olive oil alone (treatment B). The two study periods were separated by a two-week wash-out period. At baseline and at the end of each treatment, plasma levels of inflammatory markers C-reactive protein (CRP), interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-alpha), and interleukin-8 (IL-8) concentration were determined. Urinary levels of Tyr, OH-Tyr, and their metabolites were measured at the same time. Results: During combined consumption of white wine and extra-virgin olive oil (treatment A), plasma levels of CRP and IL-6 decreased from 4.1 ± 1.8 to 2.4 ± 1.9 mg/l (p < 0.05) and from 5.3 ± 3.2 to 3.4 ± 2.3 mg/l (p < 0.05) in CKD patients. CRP decreased from 2.6 ± 1.2 to 1.9 ± 0.9 mg/l (p < 0.05), and IL-6 decreased from 2.2 ± 1.8 to 1.7 ± 1.3 mg/l (p = ns) in healthy volunteers. No significant variation versus baseline was observed during treatment B. A significant increase in urinary Tyr and OH-Tyr was observed during treatment A (white wine and extra-virgin olive oil). Conclusions: Plasma markers of chronic inflammation were significantly reduced in CKD patients during the combined consumption of white wine and olive oil, suggesting a possible anti-inflammatory effect of this nutritional intervention.

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