scholarly journals Whitefly Endosymbionts: Biology, Evolution, and Plant Virus Interactions

Insects ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 775
Author(s):  
Sharon A. Andreason ◽  
Emily A. Shelby ◽  
Jeanette B. Moss ◽  
Patricia J. Moore ◽  
Allen J. Moore ◽  
...  
Keyword(s):  
Essential Amino Acids ◽  
Plant Viruses ◽  
Variable Number ◽  
Agricultural Pests ◽  
Complex Interactions ◽  
Endosymbiotic Bacteria ◽  
Sap Feeding ◽  
Virus Interactions ◽  
Plant Sap ◽  
Secondary Endosymbiont

Whiteflies (Hemiptera: Aleyrodidae) are sap-feeding global agricultural pests. These piercing-sucking insects have coevolved with intracellular endosymbiotic bacteria that help to supplement their nutrient-poor plant sap diets with essential amino acids and carotenoids. These obligate, primary endosymbionts have been incorporated into specialized organs called bacteriomes where they sometimes coexist with facultative, secondary endosymbionts. All whitefly species harbor the primary endosymbiont Candidatus Portiera aleyrodidarum and have a variable number of secondary endosymbionts. The secondary endosymbiont complement harbored by the cryptic whitefly species Bemisia tabaci is particularly complex with various assemblages of seven different genera identified to date. In this review, we discuss whitefly associated primary and secondary endosymbionts. We focus on those associated with the notorious B. tabaci species complex with emphasis on their biological characteristics and diversity. We also discuss their interactions with phytopathogenic begomoviruses (family Geminiviridae), which are transmitted exclusively by B. tabaci in a persistent-circulative manner. Unraveling the complex interactions of these endosymbionts with their insect hosts and plant viruses could lead to advancements in whitefly and whitefly transmitted virus management.

scholarly journals Mitogenomic analysis of diversity of key whitefly pests in Kenya and its implication to their sustainable management

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fathiya M. Khamis ◽  
Fidelis L. O. Ombura ◽  
Inusa J. Ajene ◽  
Komivi S. Akutse ◽  
Sevgan Subramanian ◽  
...  
Keyword(s):  
Sustainable Management ◽  
Management Strategies ◽  
Spatial Models ◽  
Abundant Species ◽  
Plant Viruses ◽  
Pest Species ◽  
Agricultural Pests ◽  
Protein Coding ◽  
Aleyrodes Proletella ◽  
Aleurodicus Dispersus

AbstractWhiteflies (Hemiptera: Aleyrodidae) are devastating agricultural pests of economic importance vectoring pathogenic plant viruses. Knowledge on their diversity and distribution in Kenya is scanty, limiting development of effective sustainable management strategies. The present study is aimed at identifying whitefly pest species present in Kenya across different agroecological zones and establish predictive models for the most abundant species in Africa. Whiteflies were sampled in Kenya from key crops known to be severely infested and identified using 16S rRNA markers and complete mitochondrial genomes. Four whitefly species were identified: Aleyrodes proletella, Aleurodicus dispersus, Bemisia afer and Trialeurodesvaporariorum, the latter being the most dominant species across all the agroecology. The assembly of complete mitogenomes and comparative analysis of all 13 protein coding genes confirmed the identities of the four species. Furthermore, prediction spatial models indicated high climatic suitability of T. vaporariorum in Africa, Europe, Central America, parts of Southern America, parts of Australia, New Zealand and Asia. Consequently, our findings provide information to guide biosecurity agencies on protocols to be adopted for precise identification of pest whitefly species in Kenya to serve as an early warning tool against T. vaporariorum invasion into unaffected areas and guide appropriate decision-making on their management.

scholarly journals Double strand RNA delivery system for plant-sap-feeding insects

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0171861 ◽  
Author(s):  
Saikat Kumar B. Ghosh ◽  
Wayne B. Hunter ◽  
Alexis L. Park ◽  
Dawn E. Gundersen-Rindal
Keyword(s):  
Delivery System ◽  
Sap Feeding ◽  
Plant Sap

The Role of Viruses in the Phytobiome

2018 ◽  
Vol 5 (1) ◽  
pp. 93-111 ◽  
Author(s):  
James E. Schoelz ◽  
Lucy R. Stewart
Keyword(s):  
Soil Bacteria ◽  
Plant Viruses ◽  
Plant Health ◽  
Plant Interactions ◽  
Current Information ◽  
Virus Interactions ◽  
The Impact ◽  
Review Current

Viruses are an important but sequence-diverse and often understudied component of the phytobiome. We succinctly review current information on how plant viruses directly affect plant health and physiology and consequently have the capacity to modulate plant interactions with their biotic and abiotic environments. Virus interactions with other biota in the phytobiome, including arthropods, fungi, and nematodes, may also impact plant health. For example, viruses interact with and modulate the interface between plants and insects. This has been extensively studied for insect-vectored plant viruses, some of which also infect their vectors. Other viruses have been shown to alter the impacts of plant-interacting phytopathogenic and nonpathogenic fungi and bacteria. Viruses that infect nematodes have also recently been discovered, but the impact of these and phage infecting soil bacteria on plant health remain largely unexplored.

scholarly journals Transitional genomes and nutritional role reversals identified for dual symbionts of adelgids (Aphidoidea: Adelgidae)

2021 ◽  
Author(s):  
Dustin T. Dial ◽  
Kathryn M. Weglarz ◽  
Akintunde O. Aremu ◽  
Nathan P. Havill ◽  
Taylor A. Pearson ◽  
...  
Keyword(s):  
Evolutionary History ◽  
Life Cycles ◽  
Fluctuating Selection ◽  
Complex Life Cycles ◽  
History Of ◽  
Sap Feeding ◽  
Selection For ◽  
Gains And Losses ◽  
Conifer Trees ◽  
Plant Sap

AbstractMany plant-sap-feeding insects have maintained a single, obligate, nutritional symbiont over the long history of their lineage. This senior symbiont may be joined by one or more junior symbionts that compensate for gaps in function incurred through genome-degradative forces. Adelgids are sap-sucking insects that feed solely on conifer trees and follow complex life cycles in which the diet fluctuates in nutrient levels. Adelgids are unusual in that both senior and junior symbionts appear to have been replaced repeatedly over their evolutionary history. Genomes can provide clues to understanding symbiont replacements, but only the dual symbionts of hemlock adelgids have been examined thus far. Here, we sequence and compare genomes of four additional dual-symbiont pairs in adelgids. We show that these symbionts are nutritional partners originating from diverse bacterial lineages and exhibiting wide variation in general genome characteristics. Although dual symbionts cooperate to produce nutrients, the balance of contributions varies widely across pairs, and total genome contents reflect a range of ages and degrees of degradation. Most symbionts appear to be in transitional states of genome reduction. Our findings support a hypothesis of periodic symbiont turnover driven by fluctuating selection for nutritional provisioning related to gains and losses of complex life cycles in their hosts.

scholarly journals Interactions between a luteovirus and the GroEL chaperonin protein of the symbiotic bacterium Buchnera aphidicola of aphids

2011 ◽  
Vol 92 (6) ◽  
pp. 1467-1474 ◽  
Author(s):  
Sophie Bouvaine ◽  
Neil Boonham ◽  
Angela E. Douglas
Keyword(s):  
Binding Site ◽  
Barley Yellow Dwarf Virus ◽  
Fat Body ◽  
Symbiotic Bacterium ◽  
Plant Viruses ◽  
Virus Binding ◽  
Buchnera Aphidicola ◽  
Virus Particles ◽  
Groel Protein ◽  
Virus Interactions

Luteoviruses and poleroviruses are important plant viruses transmitted exclusively by aphids in a circulative manner via the aphid haemolymph. A chaperonin protein, GroEL, synthesized in aphids by a symbiotic bacterium, Buchnera aphidicola, is hypothesized to bind to virus particles in the haemolymph, thereby promoting transmission. To investigate this hypothesis, the GroEL-binding site for barley yellow dwarf virus (BYDV) was determined in vitro, and the abundance of GroEL protein in different aphid tissues was investigated. Virus binding to a peptide library representing the full GroEL molecule revealed a single binding site that coincides with the site that anchors two GroEL rings to form the native GroEL tetradecamer. In the functional form of the GroEL protein, virus binding would compete with the formation of the two GroEL rings. Using a mAb raised against a Buchnera-specific GroEL epitope, GroEL was detected in Buchnera cells by immunoblotting and immunocytochemistry, but not in the aphid haemolymph, fat body or gut. From the prediction here that GroEL–virus interactions are probably severely limited by competition with other GroEL molecules, and the evidence that GroEL is not available to interact with virus particles in vivo, it is concluded that GroEL–virus interactions are unlikely to contribute to virus transmission by aphids.

scholarly journals Viruses Infecting Trees and Herbs That Produce Edible Fleshy Fruits with a Prominent Value in the Global Market: An Evolutionary Perspective

Plants ◽  
2022 ◽  
Vol 11 (2) ◽  
pp. 203
Author(s):  
Lizette Liliana Rodríguez-Verástegui ◽  
Candy Yuriria Ramírez-Zavaleta ◽  
María Fernanda Capilla-Hernández ◽  
Josefat Gregorio-Jorge
Keyword(s):  
Plant Pathogens ◽  
Current Knowledge ◽  
Plant Viruses ◽  
Global Market ◽  
Viral Diseases ◽  
Plant Growth And Development ◽  
The World ◽  
Food Commodities ◽  
Virus Interactions ◽  
Agricultural Food

Trees and herbs that produce fruits represent the most valuable agricultural food commodities in the world. However, the yield of these crops is not fully achieved due to biotic factors such as bacteria, fungi, and viruses. Viruses are capable of causing alterations in plant growth and development, thereby impacting the yield of their hosts significantly. In this work, we first compiled the world′s most comprehensive list of known edible fruits that fits our definition. Then, plant viruses infecting those trees and herbs that produce fruits with commercial importance in the global market were identified. The identified plant viruses belong to 30 families, most of them containing single-stranded RNA genomes. Importantly, we show the overall picture of the host range for some virus families following an evolutionary approach. Further, the current knowledge about plant-virus interactions, focusing on the main disorders they cause, as well as yield losses, is summarized. Additionally, since accurate diagnosis methods are of pivotal importance for viral diseases control, the current and emerging technologies for the detection of these plant pathogens are described. Finally, the most promising strategies employed to control viral diseases in the field are presented, focusing on solutions that are long-lasting.

scholarly journals Methylation at CpG sites in genomes of aphid Acyrtosiphon pisum and its endosymbiont Buchnera

2021 ◽  
Author(s):  
Mathilde Clement ◽  
Martine Da Rocha ◽  
Sandra Agnel ◽  
Guenter Raddatz ◽  
Alain Robichon ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Buchnera Aphidicola ◽  
Low Level ◽  
Endosymbiotic Bacteria ◽  
Whole Genomes ◽  
Dna Methylase ◽  
Mutualistic Relationship ◽  
Sap Feeding ◽  
Methyl Cytosine ◽  
Methylation Patterns

Pea aphid Acyrtosiphon pisum, a sap-feeding insect, has established a mutualistic relationship with an endosymbiotic bacteria (Buchnera aphidicola) that constitutes an evolutionary successful symbiosis to synthetize complex chemical compounds from a nutrient deprived diet. In this study, led by the presence of DNMT1 and a putative DNMT3 methylase in the aphid genome, we investigated the distribution of the methyl groups on 5'cytosine in CpG motifs on the whole genomes of host and endosymbiont, and looked into their correlation with gene expression. The DNA methylation turned to be present at low level in aphid (around 3% of total genomic cytosine) compared to mammals and plants, but increased to ~9% in genes. Interestingly, the reduced genome of the endosymbiont Buchnera also shows global low level of methyl cytosine despite the fact that its genome does not shelter any DNA methylase. This finding argues for the import of DNA methylase from the host to the endosymbiont. The observed differences in methylation patterns between two clonal variants (host plus endosymbiont) are reported along with the differences in their transcriptome profiles. Our data allowed to decipher a dynamic combinatorial DNA methylation and epigenetic cross talk between host and symbiont in a clonality context that might count for the aphid adaptation to environment.

scholarly journals Metabolic profile discriminates and predicts Arabidopsis susceptibility to virus under field conditions

2020 ◽  
Author(s):  
Bernadette Rubio ◽  
Olivier Fernandez ◽  
Patrick Cosson ◽  
Thierry Berton ◽  
Mélodie Caballero ◽  
...  
Keyword(s):  
Plant Virus ◽  
Field Experiments ◽  
Plant Viruses ◽  
Training Data ◽  
Natural Setting ◽  
Data Set ◽  
Primary And Secondary Metabolites ◽  
Lack Of Information ◽  
Virus Interactions ◽  
Viral Accumulation

SummaryAs obligatory parasites, plant viruses alter host cellular metabolism. There is a lack of information on the variability of virus-induced metabolic responses among genetically diverse plants in a natural context with daily changing conditions. To decipher the metabolic landscape of plant-virus interactions in a natural setting, one hundred and thirty-two and twenty-six accessions of Arabidopsis thaliana were inoculated with Turnip mosaic virus (TuMV), in two field experiments over 2 years. The accessions were phenotyped for viral accumulation, above-ground biomass, targeted and untargeted metabolic profiles. The accessions revealed quantitative response to the virus, from susceptibility to resistance. Susceptible accessions accumulate primary and secondary metabolites upon infection, at the cost of hindered growth. Orthogonal Partial Least Squares-Discriminant Analysis (OPLS-DA) revealed that the primary metabolites sucrose, glucose and glutamate discriminate susceptible and resistant accessions. Twenty-one metabolic signatures were found to significantly accumulate in resistant accessions whereas they maintained their growth at the same level as mock-inoculated plants without biomass penalty.Metabolic content was demonstrated to discriminate and to be highly predictive of the susceptibility of inoculated Arabidopsis. The PLS coefficient estimated in the training data set reveals, after cross-validation, a correlation of 0.61 between predicted and true viral accumulation. This study is the first to describe the metabolic landscape of plant-virus interactions in a natural setting and its predictive link to susceptibility. It reveals that, in this undomesticated species and in ecologically realistic conditions, growth and resistance are in a permanent conversation and provides new insights on plant-virus interactions.

scholarly journals The Effects of Different Diets and Transgenerational Stress on Acyrthosiphon pisum Development

Insects ◽  
2019 ◽  
Vol 10 (9) ◽  
pp. 260 ◽  
Author(s):  
Daniel Pers ◽  
Allison Hansen
Keyword(s):  
Host Plants ◽  
Acyrthosiphon Pisum ◽  
Life Stage ◽  
Mixed Population ◽  
Agricultural Pests ◽  
Nymphal Instar ◽  
Significant Delay ◽  
Artificial Diets ◽  
Nymphal Development ◽  
Sap Feeding

Despite the fact that sap-feeding hemipterans are major agricultural pests, little is known about the pea aphid’s (Acyrthosiphon pisum) nymphal development, compared to other insect models. Given our limited understanding of A. pisum nymphal development and variability in the naming/timing of its developmental events between different environmental conditions and studies, here, we address developmental knowledge gaps by elucidating how diet impacts A. pisum nymphal development for the LSR1 strain when it develops on its universal host plant (Vicia faba), isolated leaves, and artificial diet. Moreover, we test how plant age and transgenerational stressors, such as overcrowding and low plant vigor, can affect nymphal development. We also validate a morphological method to quickly confirm the life stage of each nymphal instar within a mixed population. Overall, we found extremely high variation in the timing of developmental events and a significant delay in nymphal (~5–25-h/instar) and pre-reproductive adult (~40-h) development when reared on isolated leaves and artificial diets, compared to intact host plants. Also, delays in development were observed when reared on older host plants (~9–17-h/event, post 2nd instar) or when previous generations were exposed to overcrowding on host plants (~20-h delay in nymph laying) compared to controls.

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