scholarly journals Efficacy of Plasma-Polymerized Allylamine Coating of Zirconia after Five Years

2020 ◽  
Vol 9 (9) ◽  
pp. 2776 ◽  
Author(s):  
Nadja Rohr ◽  
Katja Fricke ◽  
Claudia Bergemann ◽  
J Barbara Nebe ◽  
Jens Fischer
Keyword(s):  
Room Temperature ◽  
Storage Period ◽  
Cell Spreading ◽  
Osteoblastic Cells ◽  
Cell Behavior ◽  
Amino Groups ◽  
Differentiation Markers ◽  
Oxidation Processes ◽  
Human Osteoblastic Cells ◽  
Zirconia Implant

Plasma-polymerized allylamine (PPAAm) coatings of titanium enhance the cell behavior of osteoblasts. The purpose of the present study was to evaluate a PPAAm nanolayer on zirconia after a storage period of 5 years. Zirconia specimens were directly coated with PPAAm (ZA0) or stored in aseptic packages at room temperature for 5 years (ZA5). Uncoated zirconia specimens (Zmt) and the micro-structured endosseous surface of a zirconia implant (Z14) served as controls. The elemental compositions of the PPAAm coatings were characterized and the viability, spreading and gene expression of human osteoblastic cells (MG-63) were assessed. The presence of amino groups in the PPAAm layer was significantly decreased after 5 years due to oxidation processes. Cell viability after 24 h was significantly higher on uncoated specimens (Zmt) than on all other surfaces. Cell spreading after 20 min was significantly higher for Zmt = ZA0 > ZA5 > Z14, while, after 24 h, spreading also varied significantly between Zmt > ZA0 > ZA5 > Z14. The expression of the mRNA differentiation markers collagen I and osteocalcin was upregulated on untreated surfaces Z14 and Zmt when compared to the PPAAm specimens. Due to the high biocompatibility of zirconia itself, a PPAAm coating may not additionally improve cell behavior.

The Effect of Si(IV) Species Derived from Chitosan-Silicate Hydrogels on Osteoblast Behavior

2011 ◽  
Vol 493-494 ◽  
pp. 698-702 ◽  
Author(s):  
Yuki Shirosaki ◽  
Satoshi Hayakawa ◽  
Akiyoshi Osaka
Keyword(s):  
Cell Adhesion ◽  
Room Temperature ◽  
Gelation Time ◽  
Osteoblastic Cells ◽  
Amino Groups ◽  
Hybrid Hydrogels ◽  
Methoxy Groups ◽  
Gelation Behavior ◽  
Epoxy Groups ◽  
Phosphate Groups

Chitosan-GPTMS (γ-Glycidoxypropyltrimethoxysilane) hybrid hydrogels were synthesized with β-glycerophosphate (β-GP) as the additive agent. Chitosan-GPTMS sols were fluid at room temperature and transformed to hydrogel at 36.5°C in several min. The gelation time of the hydrogels was shortened by the addition of GPTMS. From NMR experiments, this gelation behavior depended on some factors, namely, electrostatic interaction between the phosphate groups of β-GP and the amino groups of chitosan chains, crosslinking between the epoxy groups of GPTMS and the amino groups of chitosan, and polycondensation of the methoxy groups of GPTMS. The Si(IV) released from the hydrogels promoted the cell adhesion and ALP activity of osteoblastic cells MG63.

EFEKTIVITAS ANTIBAKTERI BAWANG PUTIH (Allium sativum L) SEBAGAI PENGAWET ALAMI PADA IKAN LELE DUMBO (Clarias gariephinus) SEGAR

2019 ◽  
Vol 14 (1) ◽  
pp. 26
Author(s):  
Dyah Anggraeni ◽  
Nurlela Nurlela
Keyword(s):  
Room Temperature ◽  
Allium Sativum ◽  
Research Result ◽  
Clarias Gariepinus ◽  
Storage Period ◽  
The Body ◽  
Garlic Extract ◽  
African Catfish ◽  
Natural Preservatives ◽  
Colony Counter

Background: Natural preservatives are compounds produced by natural ingredients that can suppress bacterial growth and development. Natural preservatives are carried out because most of the preservatives circulating are chemicals and unsafe for the body. One of the natural preservatives is by using garlic extract (Allium sativum L).  Objective: This study is aimed to determine the effectiveness of the antibacterial garlic (Allium sativum L) as a natural preservative in fresh African catfish (Clarias gariepinus).  Method: This research used the Pour Plate iroculation method. African catfish (Clarias gariepinus) which is soaked with garlic (Allium sativum L) with a concentration of 7%, 14% and 21% for 30 minutes, then the fish will be kept at room temperature with a storage period of 24 hours and 48 hours and calculated growth in bacterial numbers with the Colony counter.  Result: Based on the research result, it was found that garlic extract (Allium sativum L) can obstruct the effectiveness of antibacterial in African catfish (Clarias gariepinus) at a concentration of 14%.

Gene expression in human osteoblastic cells from normal and heterotopic ossification

2004 ◽  
Vol 76 (1) ◽  
pp. 37-43 ◽  
Author(s):  
Christophe Chauveau ◽  
Jean-Christophe Devedjian ◽  
Marie-Claude Blary ◽  
Christophe Delecourt ◽  
Pierre Hardouin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Heterotopic Ossification ◽  
Osteoblastic Cells ◽  
Human Osteoblastic Cells

scholarly journals Studies on the Preservation of Raw Cow’s Milk by Chemical Method

1970 ◽  
Vol 42 (3) ◽  
pp. 317-326 ◽  
Author(s):  
F Rokhsana ◽  
UK Das ◽  
R Yeasmin ◽  
A Nahar ◽  
S Parveen
Keyword(s):  
Hydrogen Peroxide ◽  
Chemical Method ◽  
Room Temperature ◽  
Storage Period ◽  
Total Coliform ◽  
Human Consumption ◽  
Milk Products ◽  
E Coli ◽  
Keeping Quality ◽  
Control Milk

Studies carried out to develop a technique for the preservation of cow's milk in raw condition using hydrogen peroxide (H2O2) as a preservative. Fresh cow’s milk was collected and experiments were conducted by four treatments in order to achieve the optimum condition of storage. The treatments were with various concentration of H2O2 starting from 0.05 %, 0.1 %, 0.2 %, 0.3 %, 0.4 %, & 0.5 %. Treated milk with 0.05 % concentration of H2O2 had storage period of 20 days compared to that of the control one (5 days only) in refrigerated temperature (±8°C). On the other hand hydrogen peroxide treated milk (0.05 %) had a storage period of 8 hours at room temperature (±28°C). Results also showed that the higher concentration of H2O2 had no effect on storage period than that of control. Milk products like kheer and halawa prepared by treated milk and stored for 20 days showed almost nil growth of total coliform and E. coli which means that food products prepared from hydrogen peroxide treated milk is safe for human consumption. Key words: Raw, Storage, Hydrogen peroxide, Preservative, keeping quality, Pasteurization, deteriorated, MPN. Bangladesh J. Sci. Ind. Res. 42(3), 317-326, 2007

scholarly journals The Impact of Package Type and Temperature on the Changes in Quality and Fatty Acids Profile of Table Eggs during Their Storage

Foods ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 2047
Author(s):  
Kamil Drabik ◽  
Tomasz Próchniak ◽  
Damian Spustek ◽  
Karolina Wengerska ◽  
Justyna Batkowska
Keyword(s):  
Fatty Acids ◽  
Room Temperature ◽  
Research Work ◽  
Storage Period ◽  
Specific Weight ◽  
Water Conductivity ◽  
Shell Weight ◽  
Fatty Acids Profile ◽  
Lower Permeability ◽  
The Impact

The aim of the study was to evaluate the possibility of reducing changes in the quality of consumer hen eggs by storing them in various package type and under various temperature conditions (room and refrigeration). The material consisted of 960 chicken eggs packed in cardboard or plastic boxes, 10 pcs in each. Half of the packages were stored at room temperature (21 °C), the rest in the refrigerator (5 °C). The eggs were stored for 28 days qualitatively evaluated at 14-day intervals. The characteristics of whole egg (weight, specific weight, proportion of morphological elements, air cell depth) as well as of shell (weight, color, crushing strength, thickness, density, water conductivity), albumen (height, Haugh units, weight, pH) and yolk (weight, color, pH) were analyzed. The fatty acids profile of yolks was also evaluated as a freshness indicator. Packaging types available on the market, apart from its marketing and eggs protection function, can also influence the quality and stability of the product during storage. The use of plastic boxes can help to maintain higher eggs quality during the storage period, even after a significant extension of the storage time. Eggs stored in plastic boxes at room temperature had very similar results to those stored under refrigeration using conventional cardboard boxes. This effect is probably related to the lower permeability of plastic boxes in comparison to cardboard ones, but detailed research work in this direction is necessary to verify this relation.

Enhanced growth and matrix production by human osteoblastic cells cultured on biomaterials with negative zeta potential

Bone ◽  
2009 ◽  
Vol 44 ◽  
pp. S259 ◽  
Author(s):  
J.P. Dillon ◽  
P.J.M. Wilson ◽  
W.D. Fraser ◽  
B.K. Mwaura ◽  
M.J. Hayton ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Osteoblastic Cells ◽  
Matrix Production ◽  
Human Osteoblastic Cells ◽  
Negative Zeta Potential ◽  
Cells Cultured

Cultures of human osteoblastic cells from dialysis patients: Influence of bone turnover rate on in vitro secretion of interleukin-6 and osteoblastic cell markers

2001 ◽  
Vol 37 (1) ◽  
pp. 30-37 ◽  
Author(s):  
M. Carmen Sánchez ◽  
M. Auxiliadora Bajo ◽  
Rafael Selgas ◽  
Alberto Mate ◽  
M. Jesús Sánchez-Cabezudo ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Interleukin 6 ◽  
Turnover Rate ◽  
Osteoblastic Cells ◽  
Osteoblastic Cell ◽  
Dialysis Patients ◽  
Cell Markers ◽  
Human Osteoblastic Cells

ADRENALINE AMPOULES QUALITY ASSESSMENT–THE ISRAELI EXPERIENCE

2020 ◽  
pp. 144-146
Author(s):  
MEITAL ZUR ◽  
DAVID STEPENSKY ◽  
PAVEL GORENBEIN
Keyword(s):  
High Performance ◽  
Room Temperature ◽  
Storage Period ◽  
Clinical Effectiveness ◽  
Storage Conditions ◽  
Chiral Hplc ◽  
Drug Products ◽  
Variable Content ◽  
Israel Defense Forces ◽  
Israeli Experience

Objective: To characterize the differences in stability of L-adrenaline in adrenaline ampoules from different manufacturers that are used by the Israel Defense Forces (IDF). Methods: Adrenaline ampoules from three different vendors (Products A, B and C; 52, 13, and 19 batches, respectively) were purchased by the IDF and were stored under the recommended storage conditions (room temperature) for different time periods. The content of L-adrenaline in these samples was determined using a chiral high-performance liquid chromatography (HPLC) assay with UV detection. Results: The three analyzed drug products showed very dissimilar patterns of L-adrenaline degradation. The content of L-adrenaline in Product C was variable and declined below the 85% threshold much earlier than at the end of the 24-months storage period. Products A and B had less variable content of L-adrenaline and were more stable. Conclusion: L-adrenaline is prone to degradation in solution. Its content in adrenaline ampoules from certain vendors can decline rapidly, below the stipulated threshold, and compromise their clinical effectiveness (e. g., during resuscitation). Stability of adrenaline ampoules from individual vendors should be analyzed at different storage conditions, using a chiral HPLC-based assay, to define the shelf-life period that can differ substantially between the vendors.

scholarly journals EFFECT OF STERILIZATION BY HEATING IN THE PRESENCE OF BACTERICIDE AND BACTERIAL FILTERED MEMBRANE ON THE STABILITY OF EYE DROPS CONTAINING 0.5% CHLORAMPHENICOL AT VARIOUS pH

2019 ◽  
pp. 103-109
Author(s):  
SRI AGUNG FITRI KUSUMA ◽  
MARLINE ABDASSAH
Keyword(s):  
Room Temperature ◽  
Membrane Filter ◽  
Storage Period ◽  
Eye Drops ◽  
Percentage Reduction ◽  
Ph Change ◽  
Sterilization Method ◽  
Filter Membrane ◽  
The Stability ◽  
Antibacterial Potential

Objective: The purpose of this study was to determine a sterile 0.5% chloramphenicol eye drop formula with the best potency of antibacterial by determining the appropriate sterilization method and the supporting pH. Methods: 0.5% chloramphenicol was formulated with 0.01% thimerosal, which act as a bactericide and combines with borate buffer to produce eye drop formulas with variations in pH (6.8, 7.0 and 7.4). All formulas were stored at room temperature for 28 d and were evaluated, including: organoleptic of the preparations, sterility, pH stability, and the antibacterial potency of chloramphenicol in eye drops. Results: All dosage formulas did not undergo photodegradation reactions which were marked by no change in color until the end of the storage period. However, the formula with pH 6.8 which was sterilized by heating in a presence of bactericide, showed the presence of more particulate precipitates than in the pH 6.8 formula which was sterilized using membrane filter bacteria. However, both methods of sterilization produced sterile chloramphenicol eye drops. The preparation using a method of heat sterilization with bactericide decreased the pH greater than the preparation using a sterile bacterial filter sterilization method. C2 preparations at pH 7.0 and sterilized using the bacterial filter membrane sterilization method were more stable because they had the smallest pH change of 0.05 and the percentage reduction in antibacterial potential was smaller at 1.15%. Conclusion: The best treatment for the chloramphenicol eye drop was kept the pH formula at pH 7 and sterilized using bacterial filter membrane sterilization method.

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