scholarly journals Chitosan/Poly(vinylpyrrolidone) Matrices Obtained by Gamma-Irradiation for Skin Scaffolds: Characterization and Preliminary Cell Response Studies

Materials ◽  
2018 ◽  
Vol 11 (12) ◽  
pp. 2535 ◽  
Author(s):  
Maria Casimiro ◽  
Susana Gomes ◽  
Gabriela Rodrigues ◽  
João Leal ◽  
Luís Ferreira
Keyword(s):  
Radiation Dose ◽  
Gamma Irradiation ◽  
Fibroblast Cell ◽  
Cellular Viability ◽  
Biological Assays ◽  
Angle Measurements ◽  
Early Results ◽  
Contact Angle Measurements ◽  
Synthesis Procedure

Several studies have shown that chitosan possesses characteristics favorable for promoting dermal regeneration and accelerated wound healing. In this work we have reported the work that has been done on the development and characterization of biocompatible and biodegradable chitosan based matrices to be used as skin scaffolds. Poly(vinylpyrrrolidone) (PVP) was used as copolymer and a two steps methodology of freeze-drying and gamma irradiation was used to obtain the porous matrices. The influence of PVP content, synthesis procedure and absorbed radiation dose on matrices’ physical, chemical and structural properties was evaluated by ATR-FTIR, TGA, SEM, contact angle measurements and degradation behavior. The in vitro cellular viability and proliferation of HFFF2 fibroblast cell line was analyzed as a measure of matrices’ biocompatibility and ability to assist skin regeneration. Results show that over the studied range values, gamma-radiation dose, copolymer concentration and synthesis procedure can be used to tailor the matrices’ morphology in terms of porosity and surface roughness. Early results from biological assays evidence the biocompatibility of the prepared chitosan/PVP matrices since cells adhered to the surface of all matrices (chitosan/PVP (5%) γ-irradiated at 10 kGy presents the higher cellular viability). These features show that the resultant matrices could be a potential suitable scaffold for skin tissue regeneration.

Surface Wettability Enhances Osteoblast Adhesion on Silicon-Substituted Hydroxyapatite Thin Films

2007 ◽  
Vol 330-332 ◽  
pp. 877-880 ◽  
Author(s):  
E.S. Thian ◽  
J. Huang ◽  
Serena Best ◽  
Zoe H. Barber ◽  
William Bonfield
Keyword(s):  
Thin Films ◽  
Contact Angle ◽  
Actin Filaments ◽  
Culture Study ◽  
Cell Culture Study ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Osteoblast Adhesion ◽  
Lower Contact

Crystalline hydroxyapatite (HA) and 0.8 wt.% silicon-substituted HA (SiHA) thin films were produced using magnetron co-sputtering. These films were subjected to contact angle measurements and in vitro cell culture study using human osteoblast-like (HOB) cells. A wettability study showed that SiHA has a lower contact angle, and thus is more hydrophilic in nature, as compared to HA. Consequently, enhanced cell growth was observed on SiHA at all time-points. Furthermore, distinct and well-developed actin filaments could be seen within HOB cells on SiHA. Thus, this work demonstrated that the surface properties of the coating may be modified by the substitution of Si into the HA structure.

Surface Modification of Copolyether-Urethane Catheters with Poly(Ethylene Oxide)

1989 ◽  
Vol 12 (6) ◽  
pp. 390-394 ◽  
Author(s):  
E. Brinkman ◽  
A. Poot ◽  
T. Beugeling ◽  
L. Van Der Does ◽  
A. Bantjes
Keyword(s):  
Surface Modification ◽  
Ethylene Oxide ◽  
Blood Compatibility ◽  
Angle Measurements ◽  
Fold Reduction ◽  
Contact Angle Measurements ◽  
Platelet Deposition ◽  
Poly Ethylene Oxide ◽  
Poly Ethylene

Pellethane 2363 80A catheters were modified with poly(ethylene oxide) in order to improve their blood compatibility. Contact angle measurements showed that Pellethane 2363 80A surfaces had increased wettability after this modification. The results of in vitro blood compatibility tests showed that surface modification with poly(ethylene oxide) resulted in a five-fold reduction of platelet deposition. Activation of coagulation was not affected.

The Synthesis and Initial Studying Anticoagulant Property of O-Doped DLC Films by DC-MFCVAD

2007 ◽  
Vol 330-332 ◽  
pp. 873-876
Author(s):  
Feng Wen ◽  
Nan Huang ◽  
Hong Sun ◽  
Feng Juan Jing ◽  
An Sha Zhao
Keyword(s):  
Band Gap ◽  
Gas Flow ◽  
Blood Platelet ◽  
Vacuum Arc ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Vacuum Arc Deposition ◽  
Filtered Cathodic Vacuum Arc ◽  
Anticoagulant Property

O-doped Diamond-Like carbon (O-DLC) films were prepared by direct magnetic filtered cathodic vacuum arc deposition (DC-MFCVAD) under different O2 gas flow at room temperature (R.T.). Raman spectra were used to characterize structure of films. The wettabilities of the O-DLC films also were investigated by contact angle measurements using four common liquids. For studying films’ interaction with blood, platelet adhesion experiment in vitro was done to characterize anticoagulant property of the O- DLC films from an aspect. Furthermore, ultraviolet spectrophotometer was employed to measure the optical band gap of the synthesized films. All of the results showed that O-DLC films have good anticoagulant property, oxygen doping can prevent graphite-like tendency of DLC films and increase band gap in a way, this kind of film materials may be become a new candidated biomaterials.

Action des rayons gamma du Cobalt 60 sur la teneur en acides nucléiques et l'histogenèse des tissus de tubercule de topinambour cultivés in vitro

1983 ◽  
Vol 61 (5) ◽  
pp. 1448-1455 ◽  
Author(s):  
Janine Schaeverbeke-Sacré ◽  
Béatrice Matheron
Keyword(s):  
Radiation Dose ◽  
Gamma Irradiation ◽  
Gamma Rays ◽  
Cytological Study ◽  
Jerusalem Artichoke ◽  
Dna And Rna ◽  
Jerusalem Artichoke Tuber ◽  
Outer Area

DNA and RNA contents are studied in Jerusalem artichoke tuber explants cultured in vitro after gamma irradiation (0–5 × 105 rads (1 rad = 10 mGy)). The lower part of the explants is stimulated as soon as in contact with the medium. This stimulated area is still able to synthesize DNA and RNA up to 104 rads. An histological and cytological study shows that tissue neoformations can be observed up to 6000 rads in this outer area and that gamma rays seem to keep the cells in a "premitotic" state for a longer or shorter period according to the applied radiation dose.

Preparation of N, O-Carboxymethyl Chitosan with Different Substitutional Degree and its Application for Hemostasis

2013 ◽  
Vol 798-799 ◽  
pp. 1061-1066 ◽  
Author(s):  
Yan Wei Zhao ◽  
Lu Liu ◽  
Xiang Han ◽  
Jing Guan
Keyword(s):  
Carboxymethyl Chitosan ◽  
X Ray Diffraction ◽  
Clotting Time ◽  
X Ray ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Ft Ir ◽  
Ubbelohde Viscometer

We prepared N, O-carboxymethyl chitosans (CMCSs) with different substitutional degrees (SDs) to evaluate their effects of hemostasis, and provided experimental basis on biomedical materials. Chloroethanoic acid was used to synthesize CMCSs. The structure were characterized by Fourier transform infrared (FT-IR) and wide-angle X-ray diffraction (WXRD). Potentiometric titration and Ubbelohde viscometer were adopted to determine the SD and intrinsic viscosity of CMCSs. Contact angle measurements were investigated to determine surface wettability. Method of dynamic clotting time and coagulation test in vivo were used to evaluate their effects of hemostasis. SDs of CMCSs were from 50% to 110%. As the SD increased, molecular weight decreased. CMCS powder with SD 63% possessed excellent hemostasis both in vitro and in vivo. CMCS powder owned hemostatic capability prior to CS. CMCS powder with SD 63% (neither too high, nor too low) possessed excellent hemostasis both in vitro and in vivo.

scholarly journals Designing of Collagen Based Poly(3-hydroxybutyrate-co-4-hydroxybutyrate) Scaffolds for Tissue Engineering

2015 ◽  
Vol 2015 ◽  
pp. 1-10 ◽  
Author(s):  
S. Vigneswari ◽  
H. P. S. Abdul Khalil ◽  
A. A. Amirul
Keyword(s):  
Molar Fraction ◽  
Fibroblast Cell ◽  
Solvent System ◽  
Optimal Level ◽  
Collagen Concentration ◽  
Water Contact ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Organic Elemental Analysis ◽  
Scanning Electron

P(3HB-co-4HB) copolymer was modified using collagen by adapting dual solvent system. The surface properties of samples were characterized by Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), organic elemental analysis (CHN analysis), and water contact angle measurements. The effects of collagen concentration, scaffold thickness, and 4HB molar fraction on the hydrophilicity were optimized by the Taguchi method. The orthogonal array experiment was conducted to obtain the response for a hydrophilic scaffold. Analysis of variance (ANOVA) was used to determine the significant parameters and determine the optimal level for each parameter. The results also showed that the hydrophilicity of P(3HB-co-4HB)/collagen blend scaffolds increased as the collagen concentration increased up to 15 wt% with a molar fraction of 50 mol% at 0.1 mm scaffold thickness. The biocompatibility of the P(3HB-co-4HB)/collagen blend surface was evaluated by fibroblast cell (L929) culture. The collagen blend scaffold surfaces showed significant cell adhesion and growth as compared to P(3HB-co-4HB) copolymer scaffolds.

Bioactive Glass-Biopolymer Multilayer Coatings Fabricated by Electrophoretic Deposition Combined with Layer-by-Layer Assembly

2015 ◽  
Vol 654 ◽  
pp. 170-175 ◽  
Author(s):  
Qiang Chen ◽  
Sandra Cabanas-Polo ◽  
Ya Ping Ding ◽  
Aldo Roberto Boccaccini
Keyword(s):  
Bioactive Glass ◽  
Electrophoretic Deposition ◽  
Multilayer Coating ◽  
Multilayer Coatings ◽  
Layer By Layer ◽  
Water Contact ◽  
Characterization Methods ◽  
Angle Measurements ◽  
Contact Angle Measurements

45S5 bioactive glass (BG) based multilayer coatings on stainless steel were produced by a combination of electrophoretic deposition (EPD) and layer-by-layer (LbL) deposition. The properties of the multilayer coating were tested with different characterization methods including SEM, FTIR, XRD, laser profilometer and water contact angle measurements. Degradation and in-vitro bioactivity behaviors were tested in simulated body fluid (SBF) over different time periods. The decomposition of the coating was inhibited and the hydroxyapatite (HA) formation after short immersion period (0.5d) was confirmed. At the same time, levofloxacin, used as a model antibiotic, was incorporated into the multilayer structure for antibacterial purpose.

scholarly journals Engineered Pullulan-Collagen-Gold Nano Composite Improves Mesenchymal Stem Cells Neural Differentiation and Inflammatory Regulation

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3276
Author(s):  
Meng-Yin Yang ◽  
Bai-Shuan Liu ◽  
Hsiu-Yuan Huang ◽  
Yi-Chin Yang ◽  
Kai-Bo Chang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Physicochemical Properties ◽  
Neuronal Differentiation ◽  
Nano Composite ◽  
Angle Measurements ◽  
Contact Angle Measurements ◽  
Anti Inflammatory ◽  
Inflammatory Regulation

Tissue repair engineering supported by nanoparticles and stem cells has been demonstrated as being an efficient strategy for promoting the healing potential during the regeneration of damaged tissues. In the current study, we prepared various nanomaterials including pure Pul, pure Col, Pul–Col, Pul–Au, Pul–Col–Au, and Col–Au to investigate their physicochemical properties, biocompatibility, biological functions, differentiation capacities, and anti-inflammatory abilities through in vitro and in vivo assessments. The physicochemical properties were characterized by SEM, DLS assay, contact angle measurements, UV-Vis spectra, FTIR spectra, SERS, and XPS analysis. The biocompatibility results demonstrated Pul–Col–Au enhanced cell viability, promoted anti-oxidative ability for MSCs and HSFs, and inhibited monocyte and platelet activation. Pul–Col–Au also induced the lowest cell apoptosis and facilitated the MMP activities. Moreover, we evaluated the efficacy of Pul–Col–Au in the enhancement of neuronal differentiation capacities for MSCs. Our animal models elucidated better biocompatibility, as well as the promotion of endothelialization after implanting Pul–Col–Au for a period of one month. The above evidence indicates the excellent biocompatibility, enhancement of neuronal differentiation, and anti-inflammatory capacities, suggesting that the combination of pullulan, collagen, and Au nanoparticles can be potential nanocomposites for neuronal repair, as well as skin tissue regeneration in any further clinical treatments.

scholarly journals Synthesis and Characterization of Electrospun Composite Scaffolds Based on Chitosan-Carboxylated Graphene Oxide with Potential Biomedical Applications

Materials ◽  
2021 ◽  
Vol 14 (10) ◽  
pp. 2535
Author(s):  
Elena Cojocaru ◽  
Jana Ghitman ◽  
Elena Iuliana Biru ◽  
Gratiela Gradisteanu Pircalabioru ◽  
Eugeniu Vasile ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Cellular Proliferation ◽  
Biological Properties ◽  
Synthesis Process ◽  
Composite Scaffolds ◽  
Angle Measurements ◽  
Nanofibrous Scaffolds ◽  
Contact Angle Measurements ◽  
Poly Ethylene

This research study reports the development of chitosan/carboxylated graphene oxide (CS/GO-COOH) composite scaffolds with nanofibrous architecture using the electrospinning method. The concept of designed composite fibrous material is based on bringing together the biological properties of CS, mechanical, electrical, and biological characteristics of GO-COOH with the versatility and efficiency of ultra-modern electrospinning techniques. Three different concentrations of GO-COOH were added into a chitosan (CS)-poly(ethylene oxide) (PEO) solution (the ratio between CS/PEO was 3/7 (w/w)) and were used in the synthesis process of composite scaffolds. The effect of GO-COOH concentration on the spinnability, morphological and mechanical features, wettability, and biological properties of engineered fibrous scaffolds was thoroughly investigated. FTIR results revealed the non-covalent and covalent interactions that could take place between the system’s components. The SEM micrographs highlighted the nanofibrous architecture of scaffolds, and the presence of GO-COOH sheets along the composite CS/GO-COOH nanofibers. The size distribution graphs showed a decreasing trend in the mean diameter of composite nanofibers with the increase in GO-COOH content, from 141.40 nm for CS/PG 0.1% to 119.88 nm for CS/PG 0.5%. The dispersion of GO-COOH led to composite scaffolds with increased elasticity; the Young’s modulus of CS/PG 0.5% (84 ± 4.71 MPa) was 7.5-fold lower as compared to CS/PEO (662 ± 15.18 MPa, p < 0.0001). Contact angle measurements showed that both GO-COOH content and crosslinking step influenced the surface wettability of scaffolds, leading to materials with ~1.25-fold higher hydrophobicity. The in vitro cytocompatibility assessment showed that the designed nanofibrous scaffolds showed a reasonable cellular proliferation level after 72 h of contact with the fibroblast cells.

Functionalized Waveguides for Biological Assays

2006 ◽  
Vol 950 ◽  
Author(s):  
Aaron S. Anderson ◽  
Andrew M. Dattelbaum ◽  
Gabriel A. Montaño ◽  
Jurgen G. Schmidt ◽  
Jennifer S. Martinez ◽  
...  
Keyword(s):  
Self Assembly ◽  
Protective Antigen ◽  
Specific Binding ◽  
Biological Assays ◽  
Force Microscopy ◽  
Sensing Applications ◽  
Angle Measurements ◽  
Atomic Force ◽  
Functionalized Surfaces ◽  
Contact Angle Measurements

ABSTRACTWe report here a procedure for the functioalization of SiO2-coated, SiONx waveguides for biological assays. Surface functionalization occurs by self-assembly of an amine-terminated silane monolayer on the waveguide, followed by partial chemical modification with functionalized polyethylene glycol (PEG) groups. Functionalized surfaces were characterized by atomic force microscopy and contact angle measurements. When combined with a BSA blocking step, these functional PEG surfaces significantly reduced non-specific binding and allowed for specific binding to occur. An antibody sandwich assay for detection of Bacillus anthracis protective antigen was used to validate these surfaces for sensing applications.

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