scholarly journals Taxonomic and Metabolite Diversities of Moss-Associated Actinobacteria from Thailand

Metabolites ◽  
2021 ◽  
Vol 12 (1) ◽  
pp. 22
Author(s):  
Chadabhorn Insuk ◽  
Pornkanok Pongpamorn ◽  
Adrian Forsythe ◽  
Atsuko Matsumoto ◽  
Satoshi Ōmura ◽  
...  
Keyword(s):  
Tricalcium Phosphate ◽  
Antimicrobial Activities ◽  
Gas Chromatography Mass Spectrometry ◽  
Aureus Strain ◽  
Ecological Niches ◽  
Rrna Gene ◽  
Human Pathogens ◽  
Inhibitory Effects ◽  
Plant Growth Promoting ◽  
Indole 3 Acetic Acid

Actinobacteria are a group of ecologically important bacteria capable of producing diverse bioactive compounds. However, much remains unknown about the taxonomic and metabolic diversities of actinobacteria from many geographic regions and ecological niches. In this study, we report the isolation of actinobacteria from moss and moss-associated rhizosphere soils in Thailand. Among the 89 isolates analyzed for their bioactivities, 86 strains produced indole-3-acetic acid (IAA, ranging from 0.04 to 59.12 mg/L); 42 strains produced hydroxamate type of siderophore; 35 strains produced catecholate type of siderophore; 21 strains solubilized tricalcium phosphate; and many strains exhibited antagonistic activities against one to several of the seven selected plant, animal, and human pathogens. Overall, actinobacteria from the rhizosphere soil of mosses showed greater abilities to produce IAA and siderophores and to solubilize tricalcium phosphate than those from mosses. Among these 89 isolates, 37 were analyzed for their 16S rRNA gene sequences, which revealed their diverse phylogenetic distributions among seven genera, Streptomyces, Micromonospora, Nocardia, Actinoplanes, Saccharothrix, Streptosporangium, and Cryptosporangium. Furthermore, gas chromatography-mass spectrometry analyses of ethyl acetate crude extracts of three selected isolates with inhibitory effects against a methicillin-resistant Staphylococcus aureus strain revealed diverse metabolites with known antimicrobial activities. Together, our results demonstrate that actinobacteria from mosses in Thailand are taxonomically diverse and capable of producing a range of metabolites with plant-growth-promoting and microbial pathogen-inhibiting potentials.

scholarly journals Biotransformation of Indole to 3-Methylindole byLysinibacillus xylanilyticusStrain MA

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Pankaj Kumar Arora ◽  
Kartik Dhar ◽  
Rafael Alejandro Veloz García ◽  
Ashutosh Sharma
Keyword(s):  
Acetic Acid ◽  
Sodium Succinate ◽  
16S Rrna Gene Sequencing ◽  
Transformation Products ◽  
Degradation Pathway ◽  
Gas Chromatography Mass Spectrometry ◽  
Broth Culture ◽  
Rrna Gene ◽  
Monooxygenase Activity ◽  
Indole 3 Acetic Acid

An indole-biotransforming strain MA was identified asLysinibacillus xylanilyticuson the basis of the 16S rRNA gene sequencing. It transforms indole completely from the broth culture in the presence of an additional carbon source (i.e., sodium succinate). Gas-chromatography-mass spectrometry identified indole-3-acetamide, indole-3-acetic acid, and 3-methylindole as transformation products. Tryptophan-2-monooxygenase activity was detected in the crude extracts of indole-induced cells of strain MA, which confirms the formation of indole-3-acetamide from tryptophan in the degradation pathway of indole. On the basis of identified metabolites and enzyme assay, we have proposed a new transformation pathway for indole degradation. Indole was first transformed to indole-3-acetamide via tryptophan. Indole-3-acetamide was then transformed to indole-3-acetic acid that was decarboxylated to 3-methylindole. This is the first report of a 3-methylindole synthesis via the degradation pathway of indole.

scholarly journals Probiotic Potential ofLactobacillusStrains with Antimicrobial Activity against Some Human Pathogenic Strains

2014 ◽  
Vol 2014 ◽  
pp. 1-16 ◽  
Author(s):  
Parisa Shokryazdan ◽  
Chin Chin Sieo ◽  
Ramasamy Kalavathy ◽  
Juan Boo Liang ◽  
Noorjahan Banu Alitheen ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Activities ◽  
Rrna Gene ◽  
Human Pathogens ◽  
Bacterial Strains ◽  
Probiotic Potential ◽  
Good Ability ◽  
Acid And Bile Tolerance ◽  
Infant Feces

The objective of this study was to isolate, identify, and characterize some lactic acid bacterial strains from human milk, infant feces, and fermented grapes and dates, as potential probiotics with antimicrobial activity against some human pathogenic strains. One hundred and forty bacterial strains were isolated and, after initial identification and a preliminary screening for acid and bile tolerance, nine of the best isolates were selected and further identified using 16 S rRNA gene sequences. The nine selected isolates were then characterizedin vitrofor their probiotic characteristics and their antimicrobial activities against some human pathogens. Results showed that all nine isolates belonged to the genusLactobacillus. They were able to tolerate pH 3 for 3 h, 0.3% bile salts for 4 h, and 1.9 mg/mL pancreatic enzymes for 3 h. They exhibited good ability to attach to intestinal epithelial cells and were not resistant to the tested antibiotics. They also showed good antimicrobial activities against the tested pathogenic strains of humans, and most of them exhibited stronger antimicrobial activity than the reference strainL. caseiShirota. Thus, the nineLactobacillusstrains could be considered as potential antimicrobial probiotic strains against human pathogens and should be further studied for their human health benefits.

scholarly journals Isolation and Molecular Characterization of Antibiotic Producing Bacillus licheniformis Strains Isolated from Soil

2020 ◽  
Vol 14 (4) ◽  
pp. 2363-2370
Author(s):  
Anhar Al-Turk ◽  
Nidal Odat ◽  
Muhannad I. Massadeh
Keyword(s):  
Biochemical Characterization ◽  
Octadecenoic Acid ◽  
Salmonella Typhi ◽  
Antimicrobial Activities ◽  
Arid Area ◽  
Gas Chromatography Mass Spectrometry ◽  
Resistant Bacteria ◽  
Human Pathogens ◽  
Antibiotic Resistant

Currently, there is an increase prevalence of antibiotic-resistant bacteria worldwide. Therefore, the need for characterization of naturally occuring antibiotics with less antibiotic resistance is required. Soil resources contains valuable antibiotic producing microorganisms that increasingly being utilized for the production of suitable antibiotics. Therefore, this study aimed at identifying an antibiotic bacteria with ability of producing antibiotic that is isolated from soil samples collected from Al Zarqa provenance, an arid area in Jordan. Morphological and biochemical characterization of the isolates were carried out and found that all of the isolates belong to Bacillus genus. Further confirmation of the characterization of the bacteria was done by ribosomal RNA and PCR. The results reveal that the isolates represent Basilluslicheniformis. These bacilli were further investigated for antimicrobial activities against 6 ATCC human pathogens viz., S. aureus, S. pneumonia, Salmonella typhi., E. coli, P. mirabels and E. cloacae. Additionally, the results of Gas Chromatography Mass Spectrometry (GCMS) of ethyl acetate extracts for B. licheniformis secondary metabolites showed that they contain two main antimicrobial compounds namely Pyrrolo [1, 2-a] pyrazine-1, 4-dione,hexahydro and Trans-13-octadecenoic acid. The present work maybe suggests that soil isolates from the studied arid area include antibiotic producing strains that can be utilized commercially.

scholarly journals Screening of plant growth promoting bacteria associated with barley plants (Hordeum vulgare L.) cultivated in South Brazil

2015 ◽  
Vol 15 (2) ◽  
Author(s):  
Andress P. Pontes ◽  
Rocheli de Souza ◽  
Camille E. Granada ◽  
Luciane M.P. Passaglia
Keyword(s):  
Hordeum Vulgare ◽  
Plant Growth ◽  
Tricalcium Phosphate ◽  
Future Application ◽  
Rrna Gene ◽  
Plant Growth Promoting Bacteria ◽  
Bacterial Strains ◽  
Hordeum Vulgare L ◽  
Plant Growth Promoting ◽  
Growth Promoting

The occurrence of associations between bacteria and plant roots may be beneficial, neutral or detrimental. Plant growth promoting (PGP) bacteria form a heterogeneous group of beneficial microorganisms that can be found in the rhizosphere, the root surfaces or in association with host plant. The aim of this study was to isolate and characterize PGP bacteria associated to barley plants (Hordeum vulgare L.) aiming a future application as agricultural inoculant. One hundred and sixty bacterial strains were isolated from roots or rhizospheric soil of barley based on their growth in nitrogen-free selective media. They were evaluated for their ability to produce indolic compounds (ICs) and siderophores, and to solubilize tricalcium phosphate inin vitro assays. Most of them (74%) were able to synthesize ICs in the presence of the precursor L-tryptophan, while 57% of the isolates produced siderophores in Fe-limited liquid medium, and 17% were able to solubilize tricalcium phosphate. Thirty-two isolates possessing different PGP characteristics were identified by partial sequencing of their 16S rRNA gene. Strains belonging to Cedecea andMicrobacterium genera promoted the growth of barley plants in insoluble phosphate conditions, indicating that these bacteria could be used as bioinoculants contributing to decrease the amount of fertilizers applied in barley crops.

scholarly journals Isolation of potential fluorescent pseudomonads from Kuini (Mangifera odorata) planted soil and their potential as biofertilizer

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Seng Heng Jeffrey Lim ◽  
Nur Samahah Mohd Zainual
Keyword(s):  
Plant Growth Promoting Rhizobacteria ◽  
Antimicrobial Activities ◽  
Dilution Method ◽  
Pseudomonas Sp ◽  
Brassica Chinensis ◽  
Plant Growth Promoting ◽  
Potential Activity ◽  
Seed Germination Test ◽  
Indole 3 Acetic Acid ◽  
Soil Dilution

Pseudomonas sp. are known to be good Plant Growth Promoting Rhizobacteria (PGPR). In this study, Pseudomonas sp. were isolated from soil planted with kuini (Mangifera odorata) using soil dilution method and spread onto King’s B media. Five isolates of Pseudomonas sp. were observed to give promising results in the phytohormone and antimicrobial test conducted. These isolates are Pseudomonas sp. isolate K24pf, K29pf, K32pf, K33pf and K37pf. From the 5 potential isolates, Pseudomonas sp. isolate K29pf was chosen because it showed potential activity in producing marked amounts of Indole-3-Acetic Acid and gibberellic acid. Pseudomonas sp. isolate K29pf also produced antimicrobial activities towards Ralstonia solanacearum, Erwinia caratovora, Erwinia mallotivora and Colletotrichum gloeosporioides. Seed germination test showed that Pseudomonas sp. isolate K29pf was able to promote approximately 90% growth of Brassica chinensis seeds. Pot trial conducted showed that Treatment 3 (+OF+PGPR) was able to increase Brassica chinensis root by 36.5% and 28.4% of its biomass compared to treatment using Treatment 1 (+OF).

scholarly journals Evaluation of the Antimicrobial Efficacy of some Fermented Traditional Turkish Beverages with Probiotic Potentials

2021 ◽  
Author(s):  
Oluwaseun Temitope Aladeboyeje ◽  
Nazmiye Ozlem Sanli ◽  
Umut Buyuk
Keyword(s):  
Fruits And Vegetables ◽  
Health Benefit ◽  
Antimicrobial Activities ◽  
Rrna Gene ◽  
Human Pathogens ◽  
Strain Specificity ◽  
Antimicrobial Effects ◽  
Gene Sequence Analysis ◽  
Good Number ◽  
Microbiota Diversity

Turkey is a home country for a good number of fermented beverages derived from milk, cereals, fruits and vegetables, and several studies have reported the probiotic potentiality of these beverages. Probiotics, otherwise known as beneficial microorganisms possess the ability to exert antimicrobial effects, which is one of the most important selection criteria for their use in commercial products. In the current study, the antimicrobial activities of potential probiotic bacteria isolated from five fermented traditional Turkish beverages (boza, kefir, ayran, shalgam juice and hardaliye) were evaluated. The bacterial isolates were morphologically characterized and genotypically identified by 16S rRNA gene sequence analysis. The antimicrobial effects of the isolates against selected human pathogens were assessed using spot-on-the-lawn and agar well diffusion assays. Eighteen of the twenty-two strains displayed varying degrees of antagonism against the tested pathogens. Amongst the isolates, the strongest antimicrobial effects were exhibited by strains from boza, kefir and shalgam which can be attributed to their greater microbiota diversity. Strain specificity in the activities of the obtained isolates and specificity with the different indicator pathogens tested was observed. The impressive antimicrobial effects exhibited by boza, kefir and shalgam isolates offer a promising health benefit to consumers of these fermented probiotic products.

scholarly journals Tryptophan-Dependent Production of Indole-3-Acetic Acid (IAA) Affects Level of Plant Growth Promotion by Bacillus amyloliquefaciens FZB42

2007 ◽  
Vol 20 (6) ◽  
pp. 619-626 ◽  
Author(s):  
ElSorra E. Idris ◽  
Domingo J. Iglesias ◽  
Manuel Talon ◽  
Rainer Borriss
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Plant Growth Promotion ◽  
Bacillus Amyloliquefaciens ◽  
High Performance ◽  
Growth Promotion ◽  
Gas Chromatography Mass Spectrometry ◽  
Culture Filtrates ◽  
Plant Growth Promoting ◽  
Indole 3 Acetic Acid

Phytohormone-like acting compounds previously have been suggested to be involved in the phytostimulatory action exerted by the plant-beneficial rhizobacterium Bacillus amyloliquefaciens FZB42. Analyses by high-performance liquid chromatography and gas chromatography-mass spectrometry performed with culture filtrates of FZB42 demonstrated the presence of indole-3-acetic acid (IAA), corroborating it as one of the pivotal plant-growth-promoting substances produced by this bacterium. In the presence of 5 mM tryptophan, a fivefold increase in IAA secretion was registered. In addition, in the trp auxotrophic strains E101 (ΔtrpBA) and E102 (ΔtrpED), and in two other strains bearing knockout mutations in genes probably involved in IAA metabolism, E103 (ΔysnE, putative IAA transacetylase) and E105 (ΔyhcX, putative nitrilase), the concentration of IAA in the culture filtrates was diminished. Three of these mutant strains were less efficient in promoting plant growth, indicating that the Trp-dependent synthesis of auxins and plant growth promotion are functionally related in B. amyloliquefaciens.

scholarly journals Paenibacillus riograndensis sp. nov., a nitrogen-fixing species isolated from the rhizosphere of Triticum aestivum

2010 ◽  
Vol 60 (1) ◽  
pp. 128-133 ◽  
Author(s):  
Anelise Beneduzi ◽  
Pedro B. Costa ◽  
Márcia Parma ◽  
Itamar S. Melo ◽  
Maria Helena Bodanese-Zanettini ◽  
...  
Keyword(s):  
Triticum Aestivum ◽  
Type Strain ◽  
Plant Growth Promoting Rhizobacteria ◽  
Major Fatty Acid ◽  
Rrna Gene ◽  
Nitrogen Fixing ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid ◽  
The 16S Rrna Gene

A bacterial strain designated SBR5T was isolated from the rhizosphere of Triticum aestivum. A phylogenetic analysis based on the 16S rRNA gene sequence placed the isolate within the genus Paenibacillus, being most closely related to Paenibacillus graminis RSA19T (98.1 % similarity). The isolate was a Gram-reaction-variable, motile, facultatively anaerobic bacterium, with spores in a terminal position in cells. Starch was utilized and dihydroxyacetone and catalase were produced. Strain SBR5T displayed plant-growth-promoting rhizobacteria characteristics: the ability to fix nitrogen and to produce siderophores and indole-3-acetic acid. The DNA G+C content was 55.1 mol%. Chemotaxonomic analysis of the isolated strain revealed that MK-7 was the predominant menaquinone, while the major fatty acid was anteiso-C15 : 0. DNA–DNA hybridization values between strain SBR5T and P. graminis RSA19T, Paenibacillus odorifer TOD45T and Paenibacillus borealis KK19T were 43, 35 and 28 %, respectively. These DNA relatedness data and the results of phylogenetic and phenotypic analyses showed that strain SBR5T should be considered as the nitrogen-fixing type strain of a novel species of the genus Paenibacillus, for which the name Paenibacillus riograndensis sp. nov. is proposed. The type strain is SBR5T (=CCGB 1313T =CECT 7330T).

Effect of the endophytic plant growth promoting Enterobacter ludwigii EB4B on tomato growth

2020 ◽  
Vol 13 (2) ◽  
pp. 54-65 ◽  
Author(s):  
M.E.A. Bendaha ◽  
H.A. Belaouni
Keyword(s):  
Root Length ◽  
Biocontrol Agent ◽  
Growth Promotion ◽  
Dry Weight ◽  
Antagonistic Activity ◽  
Rrna Gene ◽  
Plant Growth Promoting ◽  
Enterobacter Ludwigii ◽  
Tomato Growth

SummaryThis study aims to develop a biocontrol agent against Fusarium oxysporum f.sp. radicis-lycopersici (FORL) in tomato. For this, a set of 23 bacterial endophytic isolates has been screened for their ability to inhibit in vitro the growth of FORL using the dual plate assay. Three isolates with the most sound antagonistic activity to FORL have been qualitatively screened for siderophore production, phosphates solubilization and indolic acetic acid (IAA) synthesis as growth promotion traits. Antagonistic values of the three candidates against FORL were respectively: 51.51 % (EB4B), 51.18 % (EB22K) and 41.40 % (EB2A). Based on 16S rRNA gene sequence analysis, the isolates EB4B and EB22K were closely related to Enterobacter ludwigii EN-119, while the strain EB2A has been assigned to Leclercia adecarboxylata NBRC 102595. The promotion of tomato growth has been assessed in vitro using the strains EB2A, EB4B and EB22K in presence of the phytopathogen FORL. The treatments with the selected isolates increased significantly the root length and dry weight. Best results were observed in isolate EB4B in terms of growth promotion in the absence of FORL, improving 326.60 % of the root length and 142.70 % of plant dry weight if compared with untreated controls. In the presence of FORL, the strain EB4B improved both root length (180.81 %) and plant dry weight (202.15 %). These results encourage further characterization of the observed beneficial effect of Enterobacter sp. EB4B for a possible use as biofertilizer and biocontrol agent against FORL.

Assessment of Cota altissima (L.) J. Gay for phytochemical composition and antioxidant, anti-inflammatory, antidiabetic and antimicrobial activities

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Gamze Göger ◽  
Muhammed Allak ◽  
Ali Şen ◽  
Fatih Göger ◽  
Mehmet Tekin ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Essential Oil ◽  
Antioxidant Activities ◽  
Antimicrobial Activities ◽  
Antidiabetic Activity ◽  
Gram Negative Bacteria ◽  
Inhibitory Effects ◽  
Dicaffeoylquinic Acid ◽  
Anti Inflammatory ◽  
Phytochemical Profiles

Abstract Phytochemical profiles of essential oil (EO), fatty acids, and n-hexane (CAH), diethyl ether (CAD), ethyl acetate (CAE) and methanol extracts (CAM) of Cota altissima L. J. Gay (syn. Anthemis altissima L.) were investigated as well as their antioxidant, anti-inflammatory, antidiabetic and antimicrobial activites. The essential oil was characterized by the content of acetophenone (35.8%) and β-caryophyllene (10.3%) by GC-MS/FID. Linoleic and oleic acid were found as main fatty acids. The major constituents of the extracts were found to be 5-caffeoylquinic acid, 3,5-dicaffeoylquinic acid, isorhamnetin glucoside, quercetin and quercetin glucoside by LC-MS/MS. Antioxidant activities of the extracts were determined by scavenging of DPPH and ABTS free radicals. Also, the inhibitory effects on lipoxygenase and α-glucosidase enzymes were determined. Antimicrobial activity was evaluated against Gram positive, Gram negative bacteria and yeast pathogens. CAM showed the highest antioxidant activity against DPPH and ABTS radicals with IC50 values of 126.60 and 144.40 μg/mL, respectively. In the anti-inflammatory activity, CAE demonstrated the highest antilipoxygenase activity with an IC50 value of 105.40 μg/mL, whereas, CAD showed the best inhibition of α-glucosidase with an IC50 value of 396.40 μg/mL in the antidiabetic activity. CAH was effective against Staphylococcus aureus at MIC = 312.5 µg/mL. This is the first report on antidiabetic, anti-inflammatory and antimicrobial activities of different extracts of C. altissima.

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