scholarly journals The Effect of Encapsulated Powder of Goji Berry (Lycium barbarum) on Growth and Survival of Probiotic Bacteria

2019 ◽  
Vol 8 (1) ◽  
pp. 57 ◽  
Author(s):  
Prodromos Skenderidis ◽  
Chrysanthi Mitsagga ◽  
Dimitrios Lampakis ◽  
Konstantinos Petrotos ◽  
Ioannis Giavasis
Keyword(s):  
Stress Tolerance ◽  
Lactobacillus Casei ◽  
Bifidobacterium Longum ◽  
Probiotic Bacteria ◽  
Lycium Barbarum ◽  
Growth And Survival ◽  
Bifidobacterium Animalis ◽  
Goji Berry ◽  
Probiotic Strains ◽  
Berry Extracts

The aim of the present work was to investigate the potential prebiotic action of Goji berry powder on selected probiotic bacteria grown in a nutritive synthetic substrate and in simulated gastric and intestinal juices. Different probiotic strains of Bifidobacterium and Lactobacillus were grown in these substrates with or without the addition of encapsulated goji berry extracts of different polysaccharide and polyphenol contents. The results proved that the addition of the extracts promoted the proliferation of probiotic strains and, in particular, increased the number of bacterial colonies of Bifidobacterium animalis subsp. lactis (Bb12), Bifidobacterium longum (Bb46), and Lactobacillus casei by 2, 0.26, and 1.34 (log cfu/mL), respectively. Furthermore, the prebiotic effect seems to be correlated to Goji berry polysaccharides and/or polyphenols, higher contents of which (under the tested concentrations) could increase the stress tolerance of B. lactis and B. longum in a simulated gastrointestinal environment. According to the findings of the present research, it can be suggested that the Goji berry encapsulated extracts could be used as prebiotic additives in food or nutraceuticals, in order to stimulate growth or protect the viability of probiotic strains of Bifidobacterium and Lactobacillus.

scholarly journals Effect of Three Polysaccharides (Inulin, and Mucilage from Chia and Flax Seeds) on the Survival of Probiotic Bacteria Encapsulated by Spray Drying

2020 ◽  
Vol 10 (13) ◽  
pp. 4623
Author(s):  
Mariela Bustamante ◽  
Loreto Laurie-Martínez ◽  
Daniela Vergara ◽  
Rocio Campos-Vega ◽  
Mónica Rubilar ◽  
...  
Keyword(s):  
Spray Drying ◽  
Lactobacillus Casei ◽  
Bifidobacterium Longum ◽  
Storage Period ◽  
Probiotic Bacteria ◽  
Second Stage ◽  
Chia Seed ◽  
Flax Seeds ◽  
Face Centered ◽  
Central Composite

Chia seed mucilage (CM), flaxseed mucilage (FM), and inulin (INL) were used as encapsulating agents to evaluate the possibility of increasing the survival of Lactobacillus casei var. rhamnosus, renamed recently to Lacticaseibacillus rhamnosus, after spray drying. Moreover, the viability of encapsulated L. rhamnosus was determined during the 250 day storage period at 4 °C. In a second stage, the conditions that maximized the survival of L. rhamnosus were evaluated on other probiotic bacteria (Lactiplantibacillus plantarum, Bifidobacterium infantis, and Bifidobacterium longum). Additionally, the viability of encapsulated probiotics during the 60 day storage period at 4 and 25 °C was evaluated. The conditions that maximize the survival of L. rhamnosus (90%) predicted by a face-centered central composite design were 14.4% w/v of maltodextrin, 0.6% w/v of CM, and 90 °C of inlet air temperature. Additionally, under these encapsulating conditions, the survival of L. plantarum, B. infantis, and B. longum was 95%, 97%, and 96%, respectively. The probiotic viability improved during storage at 4 °C but decreased at 25 °C. The highest viability values obtained for probiotics during spray drying and during storage suggest a thermoprotector effect of CM, which would ensure an optimal probiotic efficacy in the product, thus promoting its utilization in the food industry.

scholarly journals Exopolysaccharides Produced by Probiotic Strains Modify the Adhesion of Probiotics and Enteropathogens to Human Intestinal Mucus

2006 ◽  
Vol 69 (8) ◽  
pp. 2011-2015 ◽  
Author(s):  
PATRICIA RUAS-MADIEDO ◽  
MIGUEL GUEIMONDE ◽  
ABELARDO MARGOLLES ◽  
CLARA G. de los REYES-GAVILÁN ◽  
SEPPO SALMINEN
Keyword(s):  
Salmonella Enterica Serovar Typhimurium ◽  
Bifidobacterium Longum ◽  
Lactobacillus Rhamnosus ◽  
Physiological Role ◽  
Adhesion Properties ◽  
Bifidobacterium Animalis ◽  
Intestinal Mucus ◽  
Serovar Typhimurium ◽  
Probiotic Strains ◽  
Encoding Genes

Exopolysaccharides (EPSs) are exocellular polymers present in the surface of many bacteria, including Lactobacillus and Bifidobacterium. The genome sequence of several strains revealed the presence of EPS-encoding genes. However, the physiological role that EPSs play in the bacterial ecology still remains uncertain. In this study, we have assessed the effect of EPSs produced by Lactobacillus rhamnosus GG, Bifidobacterium longum NB667, and Bifidobacterium animalis IPLA-R1 on the adhesion of probiotic and enteropathogen strains to human intestinal mucus. The EPS fraction GG had no significant effect on the adhesion of L. rhamnosus GG and B. animalis IPLA-R1. However, the EPS fractions NB667 and IPLA-R1 significantly reduced the adherence of both probiotic strains. In contrast, the three EPS fractions increased the adhesion of Enterobacter sakazakii ATCC 29544 and Escherichia coli NCTC 8603. Higher adherence of Salmonella enterica serovar Typhimurium ATCC 29631 and Clostridium difficile ATCC 9689 was detected in the presence of the EPS fractions GG and NB667. In general, these effects were obtained at EPS concentrations of up to 5 mg/ml, and they were EPS dose dependent. The competitive exclusion of probiotics in the presence of EPS could suggest the involvement of these biopolymers in the adhesion to mucus. The increase in the adherence of enteropathogens could be explained if components of the pathogen surface are able to bind to specific EPSs and the bound EPSs are able to adhere to mucus. To the best of our knowledge, this is the first work reporting the effect of EPSs from probiotics on bacterial adhesion properties.

scholarly journals Exploring the bone sparing effects of postbiotics in the postmenopausal rat model

2021 ◽  
Author(s):  
Nima Montazeri-Najafabady ◽  
Younes Ghasemi ◽  
Mohammad Hossein Dabbaghmanesh ◽  
Yousef Aashoori ◽  
Pedram Talezadeh ◽  
...  
Keyword(s):  
Bone Loss ◽  
Lactobacillus Acidophilus ◽  
Lactobacillus Casei ◽  
Lactobacillus Reuteri ◽  
Bifidobacterium Longum ◽  
Bacillus Coagulans ◽  
Attractive Alternative ◽  
Sprague Dawley ◽  
Ovx Rats ◽  
Probiotic Strains

Abstract Background Postmenopausal osteoporosis is a concern of health organizations, and current treatments do not seem enough. Postbiotics as bioactive compounds produced by probiotics may be an attractive alternative for bone health. In this study, we prepared, formulated, and compared the effects of cell lysate and supernatant of five native probiotic strains (Lactobacillus acidophilus, Lactobacillus reuteri, Lactobacillus casei, Bifidobacterium longum, and Bacillus coagulans) in ovariectomized (OVX) rats. Methods The probiotic strains were isolated, and their cell-free supernatants and biomasses as postbiotics were extracted and formulated using standard microbial processes. The Sprague-Dawley rats were fed by 1 × 109 CFU/ml/day postbiotic preparations and ovariectomized. Dual-energy X-ray absorptiometry (DEXA) scans were accomplished to evaluate femur, spine, and tibia BMD. The serum biochemical markers [calcium, phosphorus, and alkaline phosphatase] were assessed. Results Postbiotics could considerably improve the global and femur area in OVX rats. In the case of global BMC, Lactobacillus casei lysate and supernatant, Bacillus coagulans lysate and supernatant, lysate of Bifidobacterium longum and Lactobacillus acidophilus, and Lactobacillus reuteri supernatant significantly increased BMC. We found Bacillus coagulans supernatant meaningfully enriched tibia BMC. Conclusion Postbiotic could ameliorate bone loss resulting from estrogen deficiency. Also, the effects of postbiotics on different bone sites are strain-dependent. More clinical studies need to explore the optimal administrative dose and duration of the specific postbiotics in protecting bone loss.

scholarly journals Сarbohydrate metabolism parameters under the glutamate-induced obesity in rats and concominant correction with probiotic strains of lactobacilli and bifidobacteria

2015 ◽  
Vol 70 (2) ◽  
pp. 20-23
Author(s):  
I. Leshchenko ◽  
O. Virchenko ◽  
O. Gadiliya ◽  
T. Falalyeyeva ◽  
L. Lazarenko
Keyword(s):  
Insulin Resistance ◽  
Lactobacillus Casei ◽  
Monosodium Glutamate ◽  
Resistance Index ◽  
Insulin Levels ◽  
Bifidobacterium Animalis ◽  
Insulin Resistance Index ◽  
Probiotic Strains ◽  
Neonatal Administration

To find out the effect of probiotic strains on insulin resistance induced by glutamate-induced obesity in rats. Obesity was induced by neonatal administration of monosodium glutamate (4 mg/g,s.c.) at 2, 4, 6, 8, 10-day life. The introduction of probiotics started at the end of 4 week after birth and continued intermittently for 2-week courses. After 4 months, the rats of all groups glucose and insulin levels and insulin resistance index HOMA were analyzed. Neonatal administration of monosodium glutamate leads to the development of insulin resistance in rats 4 months of age. The periodic introduction of the combined probiotics prevented the development insulin resistance in rats. The most significant effect was revealed in the group of animals treated with the combined probiotic strains Lactobacillus casei IMVB-7280, Bifidobacterium animalis VKL, Bifidobacterium animalis VKB. The results suggest the effectiveness of the use of probiotic strains of bifidobacteria and to prevent the development of insulin resistance.

scholarly journals Prophylactic effect of lactobacilli and bifidobacteria probiotic strains on experimental bacterial vaginitis

2019 ◽  
Vol 27 (2) ◽  
pp. 170-176
Author(s):  
L. P. Babenko ◽  
L. M. Lazarenko ◽  
R. V. Bubnov ◽  
M. J. Spivak
Keyword(s):  
Lactobacillus Casei ◽  
Inflammatory Diseases ◽  
Coliform Bacteria ◽  
Prophylactic Effect ◽  
Microscopic Fungi ◽  
Preventive Effect ◽  
Genitourinary System ◽  
Bifidobacterium Animalis ◽  
Probiotic Strains ◽  
Spread Of Infection

The objective of the study was determining the prophylactic effect of Lactobacillus casei IMV B-7280, L. acidophilus IMV B-7279, L. delbrueckii subsp. bulgaricus IMV B-7281, Bifidobacterium animalis VKL and B. animalis VKB probiotic strains on experimental vaginitis in BALB/c mice induced by Staphylococcus aureus 8325-4. The infection with S. aureus 8325-4 caused an imbalance of microbiota in the vagina and intestine, as evidenced by an increase in the number of opportunistic microorganisms and a decrease in the amount of lactobacilli and bifidobacteria. L. casei IMV B-7280, B. animalis VKL and B. animalis VKB probiotic strains altered the microbiota spectrum of the vagina and intestine of Staphylococcus-infected mice: the amount of Lactobacillus and bifidobacteria increased with the reduction of the number of opportunistic microorganisms. Also under the influence of these strains, the normalization of the microbiota spectrum typical for vagina and intestine was observed in different periods of observation – in the intestines of mice the number of coliform bacteria increased, the number of microscopic fungi, streptococci and staphylococci decreased; in the vagina, the number of coliform bacteria and microscopic fungi decreased, the number of streptococci normalized. Rapid elimination of S. aureus 8325-4 from the vagina and prevention of the spread of infection to the intestine were observed after use of probiotics. Preventive effect of L. acidophilus IMV B-7279 and L. delbrueckii subsp. bulgaricus IMV B-7281 for bacterial vaginitis in mice was less effective. So, the target probiotic strains L. casei IMV B-7280, B. animalis VKL and B. animalis VKB are promising for the creation of highly effective novel probiotic drugs that can be used for directed prevention of infectious and inflammatory diseases of the genitourinary system caused by pathogenic and opportunistic microorganisms.

scholarly journals Exploring the bone sparing effects of postbiotics in the post-menopausal rat model

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Nima Montazeri-Najafabady ◽  
Younes Ghasemi ◽  
Mohammad Hossein Dabbaghmanesh ◽  
Yousef Ashoori ◽  
Pedram Talezadeh ◽  
...  
Keyword(s):  
Bone Loss ◽  
Lactobacillus Acidophilus ◽  
Lactobacillus Casei ◽  
Lactobacillus Reuteri ◽  
Bifidobacterium Longum ◽  
Bacillus Coagulans ◽  
Mineral Density ◽  
Ovx Rats ◽  
Probiotic Strains ◽  
Post Menopausal

Abstract Background Post-menopausal osteoporosis is a concern of health organizations, and current treatments do not seem enough. Postbiotics as bioactive compounds produced by probiotics may be an attractive alternative for bone health. In this study, we prepared, formulated, and compared the effects of cell lysate and supernatant of five native probiotic strains (Lactobacillus acidophilus, Lactobacillus reuteri, Lactobacillus casei, Bifidobacterium longum, and Bacillus coagulans) in ovariectomized (OVX) rats. Methods The probiotic strains were isolated, and their cell-free supernatants and biomasses as postbiotics were extracted and formulated using standard microbial processes. The Sprague-Dawley rats were fed by 1 × 109 CFU/ml/day postbiotic preparations for 4 weeks immediately after ovariectomy. Dual-energy X-ray absorptiometry (DEXA) scans were accomplished to evaluate femur, spine, and tibia BMD. The serum biochemical markers [calcium, phosphorus, and alkaline phosphatase] were assessed. Results Postbiotics could considerably improve the global and femur area in OVX rats. In the case of global bone mineral density (BMD), Lactobacillus casei lysate and supernatant, Bacillus coagulans lysate and supernatant, lysate of Bifidobacterium longum and Lactobacillus acidophilus, and Lactobacillus reuteri supernatant significantly increased BMD. We found Bacillus coagulans supernatant meaningfully enriched tibia BMD. Conclusion Postbiotic could ameliorate bone loss resulting from estrogen deficiency. Also, the effects of postbiotics on different bone sites are strain-dependent. More clinical studies need to explore the optimal administrative dose and duration of the specific postbiotics in protecting bone loss.

scholarly journals Viable and Heat-Killed Probiotic Strains Improve Oral Immunity by Elevating the IgA Concentration in the Oral Mucosa

2021 ◽  
Author(s):  
Wen-Yang Lin ◽  
Yi-Wei Kuo ◽  
Ching-Wei Chen ◽  
Yu-Fen Huang ◽  
Chen-Hung Hsu ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
The Body ◽  
Effective Dosage ◽  
Tgf Beta ◽  
Oral Tablet ◽  
Beneficial Effects ◽  
Bifidobacterium Animalis ◽  
Probiotic Strains ◽  
Oral Pathogens

AbstractOral-nasal mucosal immunity plays a crucial role in protecting the body against bacterial and viral invasion. Safe probiotic products have been used to enhance human immunity and oral health. In this study, we verified the beneficial effects of mixed viable probiotic tablets, consisting of Lactobacillus salivarius subsp. salicinius AP-32, Bifidobacterium animalis subsp. lactis CP-9, and Lactobacillus paracasei ET-66, and heat-killed probiotic tablets, consisting of L. salivarius subsp. salicinius AP-32 and L. paracasei ET-66, on oral immunity among 45 healthy participants. Participants were randomly divided into viable probiotic, heat-killed probiotic, and placebo groups. The administration of treatment lasted for 4 weeks. Saliva samples were collected at Weeks 0, 2, 4, and 6, and Lactobacillus, Bifidobacterium and Streptococcus mutans populations and IgA concentration were measured. IgA concentrations, levels of TGF-beta and IL-10 in PBMCs cells were quantified by ELISA method. Results showed that salivary IgA levels were significantly increased on administration of both the viable (119.30 ± 12.63%, ***P < 0.001) and heat-killed (116.78 ± 12.28%, ***P < 0.001) probiotics for 4 weeks. Among three probiotic strains, AP-32 would effectively increase the levels of TGF-beta and IL-10 in PBMCs. The oral pathogen Streptococcus mutans was significantly reduced on viable probiotic tablet administration (49.60 ± 31.01%, ***P < 0.001). The in vitro antibacterial test confirmed that viable probiotics effectively limited the survival rate of oral pathogens. Thus, this clinical pilot study demonstrated that oral probiotic tablets both in viable form or heat-killed form could exert beneficial effects on oral immunity via IL-10, TGB-beta mediated IgA secretion. The effective dosage of viable probiotic content in the oral tablet was 109 CFUs/g and the heat-killed oral tablet was 1 × 1010 cells/g.

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