scholarly journals Technological Parameters, Anti-Listeria Activity, Biogenic Amines Formation and Degradation Ability of L. plantarum Strains Isolated from Sheep-Fermented Sausage

2021 ◽  
Vol 9 (9) ◽  
pp. 1895
Author(s):  
Nicoletta P. Mangia ◽  
Michele Cottu ◽  
Maria E. Mura ◽  
Marco A. Murgia ◽  
Giuseppe Blaiotta
Keyword(s):  
Biogenic Amines ◽  
Lipolytic Activity ◽  
Inhibition Zone ◽  
Point Of View ◽  
Technological Parameters ◽  
Fermented Sausage ◽  
Oxidase Gene ◽  
Almost All ◽  
Copper Oxidase ◽  
Degradation Ability

The aim of this work was to identify and characterize, from a technological and safety point of view, the lactic acid bacteria (LAB) isolated from traditional sheep-fermented sausage. First, LABs were identified then were screened for some technological parameters such as acidifying and growth ability, proteolytic and lipolytic activity and for antimicrobial activity. Finally, biogenic amine production and degradation abilities were also evaluated. This research reveals the predominance of Lactiplantibacillus (L.) plantarum on LAB community. Almost all L. plantarum strains were active against Listeria monocytogenes strains (inhibition zone diameters > 1 cm). None of the tested strains were positive in histidine (hdcA), lysine (ldc) and tyrosine (tyrdc) decarboxylase genes and only one (L. plantarum PT9-2) was positive to the agmatine deiminase (agdi) gene. Furthermore, given the positive results of the sufl (multi-copper oxidase) gene detection, all strains showed a potential degradation ability of biogenic amines.

scholarly journals How ethical challenges of covert observations can be met in practice

2021 ◽  
pp. 174701612110082
Author(s):  
Nicole Podschuweit
Keyword(s):  
Social Research ◽  
Role Playing ◽  
Great Distance ◽  
Point Of View ◽  
Ethical Challenges ◽  
Research Subjects ◽  
Written Information ◽  
Ethical Debate ◽  
The People ◽  
Almost All

This paper aims to bring into the ethical debate on covert research two aspects that are neglected to date: the perspective of the research subjects and the special responsibility of investigators towards their observers. Both aspects are falling behind, especially in quantitative social research. From a methodological point of view, quantitative forms of covert observation involve a great distance between the researcher and the research subjects. When human observers are involved, the focus is usually on the reliable application of the measuring instrument. Therefore, herein, a quantitative study is used as an example to show how the protection needs of both the observed persons and the observers can be met in practice. The study involved 40 student observers who covertly captured everyday conversations in real-world settings (e.g. in cafés or trains) by a highly standardised observation scheme. The study suggests that the anonymity of the research subjects and their trust in the observers are crucial for their subsequent consent. However, many participants showed only little or even no interest in the written information they were provided. Further, this study strongly emphasises how mentally stressful covert observations are to the observers. Almost all observers were worried in advance that the people they were observing would prematurely blow their cover and confront them. Role-playing and in-depth discussions in teams are good strategies to alleviate such and other fears and to prepare student assistants well for their demanding work in the field.

Detection of Biogenic Amine Producer Bacteria in a Typical Italian Goat Cheese

2008 ◽  
Vol 71 (1) ◽  
pp. 205-209 ◽  
Author(s):  
SILVIA BONETTA ◽  
SARA BONETTA ◽  
ELISABETTA CARRARO ◽  
JEAN DANIEL COÏSSON ◽  
FABIANO TRAVAGLIA ◽  
...  
Keyword(s):  
Biogenic Amines ◽  
Enterococcus Faecalis ◽  
Biogenic Amine ◽  
Pediococcus Acidilactici ◽  
Bacterial Strains ◽  
Goat Cheese ◽  
Low Concentrations ◽  
Amine Content ◽  
High Concentrations ◽  
Almost All

The aim of this study was to research decarboxylating bacterial strains and biogenic amine content in a typical Italian goat cheese (Robiola di Roccaverano). The study was performed on fresh and ripened samples of goat cheese manufactured from industrial and artisanal producers. Sixty-seven bacterial strains isolated showed decarboxylating activity, and Enterococcus faecalis was the most widespread decarboxylating species in all artisanal and industrial products. Pediococcus acidilactici and Enterococcus malodoratus were also identified as biogenic amine producers in Robiola di Roccaverano cheese. All the E. faecalis strains isolated in this study were able to decarboxylate tyrosine. Tyramine was the most abundant biogenic amine in cheese samples, while histamine was the most widespread. High amounts of these two biogenic amines were found in ripened samples (up to 2,067 mg/kg for tyramine and 1,786 mg/kg for histamine), whereas 2-phenylethylamine and tryptamine were present in almost all ripened cheeses at low concentrations. The detection of strains producing biogenic amines and the high concentrations of tyramine and histamine found in ripened Robiola di Roccaverano could represent a potential risk to the consumer.

Biogenic Amine Index for Freshness Evaluation in Iced Mediterranean Hake (Merluccius merluccius)

2005 ◽  
Vol 68 (11) ◽  
pp. 2433-2438 ◽  
Author(s):  
S. BAIXAS-NOGUERAS ◽  
S. BOVER-CID ◽  
M. T. VECIANA-NOGUÉS ◽  
A. MARINÉ-FONT ◽  
M. C. VIDAL-CAROU
Keyword(s):  
Biogenic Amines ◽  
Biogenic Amine ◽  
Sensory Analysis ◽  
Pelagic Fish ◽  
Point Of View ◽  
Ice Storage ◽  
Complementary Information ◽  
Merluccius Merluccius ◽  
Sensory Data ◽  
Chilling Temperatures

Biogenic amine accumulation was studied during the ice storage of Mediterranean hake. Sensory analysis and counts of Shewanella, Pseudomonas, enterobacteria, psychrotrophic, and mesophilic bacteria provided complementary information on hake spoilage. Putrescine and cadaverine were the main amines accumulated, whereas histamine and tyramine were minor amines but had qualitative interest from the hygienic point of view. Although all biogenic amines were less abundant than in pelagic fish, they may also be used as indicators of freshness and/or spoilage in hake. Cadaverine was the amine best correlated with Shewanella, which was the specific spoilage organism. Therefore, cadaverine may be regarded as the specific spoilage biogenic amine for hake stored at chilling temperatures. However, the biogenic amine index, which considers cadaverine, putrescine, histamine, and tyramine, has several advantages as an indicator of hake quality. Taking into account sensory data, an acceptability limit of the biogenic amine index could be established in 15 to 20 μg/g.

Applicability of Bacteriocinogenic Lactobacillus pentosus 31-1 as a Novel Functional Starter Culture or Coculture for Fermented Sausage Manufacture

2010 ◽  
Vol 73 (2) ◽  
pp. 292-298 ◽  
Author(s):  
GUORONG LIU ◽  
MANSEL W. GRIFFITHS ◽  
NAN SHANG ◽  
SHANGWU CHEN ◽  
PINGLAN LI
Keyword(s):  
Sensory Quality ◽  
Lipolytic Activity ◽  
Starter Culture ◽  
Meat Product ◽  
Fatty Acid Analysis ◽  
Fermented Sausage ◽  
Lactobacillus Pentosus ◽  
Fermented Sausages ◽  
Challenge Tests ◽  
Sensory Profiles

The technological feasibility of producing fermented sausages using the bacteriocin-producing Lactobacillus pentosus 31-1, isolated from a traditional Chinese fermented meat product (Xuanwei ham), was evaluated. Strain 31-1 was used both as a single starter and in coculture for manufacture of fermented sausages. The microbiological and physicochemical properties (color, texture, and sensory quality) and the production of bacteriocin during ripening of these products were compared with those characteristics of sausages produced with a commercial meat starter. Challenge tests were performed using Listeria innocua or Staphylococcus aureus as target strains. The addition of L. pentosus 31-1 can significantly reduce L. innocua and S. aureus populations during all ripening phases. Free amino acid and free fatty acid analysis suggested that strain 31-1 might have proteolytic and lipolytic activity. The use of this strain resulted in a final product with a brighter surface and better texture and sensory profiles. A maximum bacteriocin (pentocin 31-1) concentration of 640 AU/g was detected in homogenized sausages with added L. pentosus 31-1. The bacteriocin-producing strain L. pentosus 31-1 could be used as a novel functional starter culture or coculture for sausage fermentation.

Bernhard Riemann’s legacy of 1859

2009 ◽  
Vol 93 (528) ◽  
pp. 468-475
Author(s):  
Graham Hoare
Keyword(s):  
Number Theory ◽  
Mathematical Physics ◽  
Point Of View ◽  
German Version ◽  
Profound Impact ◽  
Almost All ◽  
University Of Göttingen ◽  
The University ◽  
Modern Mathematics ◽  
Mathematics And Physics

The German version of Riemann’s Collected Works is confined to a single volume of 690 pages. Even so, this volume has had an abiding and profound impact on modern mathematics and physics, as we shall see. In fifteen years of activity, from 1851, when he gained his doctorate at the University of Göttingen, to his death in 1866, two months short of his fortieth birthday, Riemann contributed to almost all areas of mathematics. He perceived mathematics from the analytic point of view and used analysis to illuminate subjects as diverse as number theory and geometry. Although regarded principally as a mathematician Riemann had an abiding interest in physics and researched significantly in the methods of mathematical physics, particularly in the area of partial differential equations.

PHYTOCHEMICAL INVESTIGATION AND ANTIMICROBIAL PROPERTIES OF DIOSCOREA BULBIFERA TUBER

2017 ◽  
Vol 10 (12) ◽  
pp. 317
Author(s):  
Dahiya P
Keyword(s):  
Antimicrobial Properties ◽  
Inhibition Zone ◽  
Multidrug Resistant ◽  
Diffusion Method ◽  
Phytochemical Analysis ◽  
Phytochemical Investigation ◽  
Dioscorea Bulbifera ◽  
Acinetobacter Sp ◽  
Almost All

  Objective: The inhibitory properties of successive extracts from Dioscorea bulbifera (Dioscoreaceae) tubers have been evaluated for the presence of phytochemical constituents and antimicrobial efficacy against multidrug-resistant (MDR) clinical isolates was evaluated.Methods: The tuber of D. bulbifera was oven dried and extracted successively with n-hexane, chloroform, methanol, ethanol, and water. The antimicrobial potential of successive extracts against MDR isolates was studied by agar well-diffusion method. Qualitative phytochemical analysis was performed.Results: Qualitative phytochemical analysis demonstrated the presence of steroids, flavonoids, cardiac glycosides, saponins, and reducing sugars in almost all the extracts tested. Anthraquinones, phlobatanins, and tannins were not reported in any extracts tested. The in vitro antimicrobial activity of various solvents and water extracts of D. bulbifera was further investigated against ten MDR bacteria and three fungi, respectively. Aqueous and chloroform extracts were found to be more potent being capable of exerting significant inhibitory activities against the majority of the isolates such as Escherichia coli, Acinetobacter sp., Salmonella paratyphi, Klebsiella pneumoniae, and Candida albicans. The highest inhibitory activity was observed for K. pneumoniae with wide inhibition zone diameters (17 ± 0.15 mm), followed by E. coli 1(13 ± 0.11) mm, and Acinetobacter sp. (11 ± 0.12).Conclusion: Based on the present study, the extracts of D. bulbifera tubers have shown excellent activity against MDR microbial cultures tested. Further study is recommended for clinical evaluation, of the efficacy of crude extract in herbal medicine that can serve as a base for the development of novel potent drugs and phytomedicines.

scholarly journals Social Order and Practical Wisdom of Walking in a Crowd

2018 ◽  
Vol 24 (4) ◽  
pp. 8-29
Author(s):  
Andrei M. Korbut
Keyword(s):  
Social Order ◽  
Practical Wisdom ◽  
Point Of View ◽  
Joint Production ◽  
Social Phenomena ◽  
Social Transformations ◽  
Sociological Approach ◽  
Everyday Practices ◽  
Phenomenal Field ◽  
Almost All

The article suggests returning to the “crowd” as an object of sociological analysis. Crowds have attracted early sociologists because crowds were visual embodiments of social forces that surpass individuals and also served as a symbol of the profound social transformations which were taking place in the late 19th and early 20th centuries. Analyzing crowds allowed for the first sociologists (G. Simmel, R. Park, M. Weber, E. Durkheim) to oppose the psychological interpretation of mass social phenomena with a purely sociological approach. However, in the second half of the 20th century sociologists had lost almost all interest in the crowd, as it did not meet the interests of researchers of “large” social structures, nor the interests of the proponents of interactionist approaches. This article shows that the crowd can again be made interesting for sociology if we were to consider it from the point of view of the everyday practices of the participants. In these everyday practices a specific form of phronesis, i.e. practical wisdom, technical skill coupled with moral judgment about which action is good and which is not, is implemented. It is shown here that the study of the practical wisdom of walking in a crowd requires special concepts and methods that can be found in phenomenology and ethnomethodology. The article suggests using three such concepts for the analysis of crowds: phenomenal field, oriented object, and figuration of details. With the help of these concepts, the methods of the crowd’s situated social order production are analyzed in relation to the management of speed and trajectories of movement, following one another, walkers’ stopping and slowing down, and joining the crowd. This analysis shows that the joint production of the crowd’s social order by its participants is a situated practice, i.e. it consists of making the local scenes of everyday life familiar and accountable, and of assessing the local adequacy of the actions performed.

scholarly journals Influence of Technological Parameters on the Emision on DLP 3D Printing Process

2020 ◽  
Vol 70 (12) ◽  
pp. 4387-4392
Keyword(s):  
3D Printing ◽  
Point Of View ◽  
Particulate Emissions ◽  
Technological Parameters ◽  
Printing Process ◽  
Laboratory Activity ◽  
Emission Point ◽  
Measurement Methodology ◽  
Made In

The present work addresses the issue of emissions requires it made in resin polymerization processes at 3D digital light process (DLP) printing. From an emission point of view, both particulate and chemical emissions are analysed in the form of gases during the DLP printing process. In the paper, we present first the element, which are study. In second part of the paper, we presented the printer, material for printing, measuring apparatus for emission and measurement methodology. In the three part of paper, we made the determinations for gas emissions. Will follow the determinations for particulate emissions. In the final chapter, the data generated by the printing emissions related to the problems specific to the laboratory activity and it has made the specific conclusion in rapport with the printing process. Keywords: 3D printing; emission particles; air pollution, resin material, DLP printing

scholarly journals SISI NEGATIF GAME ONLINE PERSPEKTIF ISLAM DAN PSIKOLOGI ISLAM

2021 ◽  
Vol 5 (2) ◽  
pp. 153
Author(s):  
Khotibul Umam ◽  
Abdul Muhid
Keyword(s):  
Literature Review ◽  
Life Style ◽  
Negative Impact ◽  
Technological Development ◽  
Human Life ◽  
Online Games ◽  
Point Of View ◽  
Technological Advances ◽  
Islamic Psychology ◽  
Almost All

Technological development has changed human life style. Child's world that was once filled with traditional games has now been eroded by technological advances. Nowadays online games that replace traditional games are not only favored by children, almost all levels of human age like playing online games. But behind it all there is a negative impact that haunts its users. This study aims to reveal the negative side of using online games from the point of view of Islam and Islamic Psychology. The technique used is literature review by compiling some previous articles. The results showed that excessive use of online games will give a bad impact on the physical and psychological users, such as visual impairment, sleep disturbance, addiction, violence and stress. The use of online games is also considered more disadvantage than the benefits and it’s seen as an activity that wastes time, and it is prohibited in Islam.

scholarly journals Complexes for continuous monitoring of drilling fluid parameters during drilling

2021 ◽  
Vol 6 (3) ◽  
pp. 144-151
Author(s):  
Sergey V. Lakhtionov ◽  
Ivan S. Chumakov ◽  
Sergey G. Filinkov ◽  
Dmitry M. Chukin ◽  
Evgeny N. Ishmetyev
Keyword(s):  
Continuous Monitoring ◽  
Drilling Fluid ◽  
Point Of View ◽  
Complete Analysis ◽  
Drilling Fluids ◽  
Automatic Mode ◽  
Design Values ◽  
Drilling Rigs ◽  
Almost All ◽  
Fluid Parameters

Background. The article provides an overview of existing complexes (units) for continuous monitoring of drilling fluid parameters in automatic mode. Aim. To justify the need to develop a complex (module) that will allow combining existing technologies and making a step forward in the field of process automation in terms of monitoring the parameters of drilling fluids. Materials and methods. In the current realities of well construction, the control of drilling fluid parameters on almost all drilling rigs operating on the territory of Russia (possibly with the exception of a few off shore projects) is carried out by the work of a solution engineer, usually a representative of a service company. The analysis of the parameters, depending on the number of personnel, the speed of penetration, the complexity or importance of the well, can be carried out from 2 to 6 times a day [1, 2]. This means a complete analysis, rather than monitoring the density and conditional viscosity, which can be measured by a representative of the drilling crew, for rapid response, and with greater frequency. Due to such a low measurement discreteness, there is a high probability of a significant deviation of the drilling fluid parameters from the design values. As a result, the probability of various complications, both geological and technological, increase significantly. Results. During the analysis of information from open sources, the most promising complexes (modules) from the point of view of application in the current conditions were identified, their positive and negative sides were evaluated. As a result of the conducted review of open sources, the most promising complexes (modules) in terms of application in the current conditions are identified, the positive and negative sides of the systems under consideration are displayed, and the need to develop a complex (module) that will combine all the best that is available today and make a qualitative step forward in the field of “peopleless” technologies used during drilling wells in terms of monitoring the parameters of drilling fluids is justified. Conclusions. The necessity of developing a complex (module) for automating processes in terms of monitoring the parameters of drilling fluids is justified.

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