scholarly journals Comparative Pharmacokinetics of Gallic Acid, Protocatechuic Acid, and Quercitrin in Normal and Pyelonephritis Rats after Oral Administration of a Polygonum capitatum Extract

Molecules ◽  
2019 ◽  
Vol 24 (21) ◽  
pp. 3873 ◽  
Author(s):  
Yong Huang ◽  
Zuying Zhou ◽  
Wu Yang ◽  
Zipeng Gong ◽  
Yueting Li ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Protocatechuic Acid ◽  
Ultra Performance Liquid Chromatography ◽  
Pharmacokinetic Parameters ◽  
Bioactive Constituents ◽  
Time Points ◽  
Pharmacokinetic Properties ◽  
Liquid Chromatography Tandem Mass ◽  
Tract Infections ◽  
Pharmacokinetic Behavior

Polygonum capitatum Buch.-Ham. ex D. Don is traditionally used by Hmong for the treatment of urinary tract infections and pyelonephritis. Information regarding the pharmacokinetic behavior of the extract in the condition of pyelonephritis is lacking. In the present study, we aimed to compare the pharmacokinetic properties of gallic acid (GA), protocatechuic acid (PCA), and quercitrin (QR)—the main bioactive constituents in the herb—in normal and pyelonephritis rats. The plasma samples were collected at various time points after administration of a single dose of Polygonum capitatum extract. The plasma level of GA, PCA, and QR at the designed time points was determined by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) and drug concentration versus time plots were constructed to estimate the pharmacokinetic parameters. The AUC(0-t), AUC(0-∞), MRT(0-t), and CL of GA, PCA, and QR in pyelonephritis rats was significantly different from those of the normal rats. The results indicated that the three constituents have higher rate of uptake and slower rate of elimination in the rats with pyelonephritis, suggesting altered rate and extent of drug metabolism.

scholarly journals Determination of chlorogenic and gallic acids by UPLC-MS/MS

2013 ◽  
Vol 59 (1) ◽  
pp. 7-16
Author(s):  
Agnieszka Gryszczyńska ◽  
Bogna Opala ◽  
Zdzisław Łowicki ◽  
Anna Krajewska-Patan ◽  
Waldemar Buchwald ◽  
...  
Keyword(s):  
Gallic Acid ◽  
Chromatographic Method ◽  
Ultra Performance Liquid Chromatography ◽  
Polyphenolic Compounds ◽  
Qualitative And Quantitative ◽  
Chromatography Tandem Mass Spectrometry ◽  
Liquid Chromatography Tandem Mass ◽  
Quantitative Analyses ◽  
Polyphenolic Acids

Summary The aim of our study were qualitative and quantitative analyses of two polyphenolic acids: chlorogenic and gallic acids. These compounds were determined in two species of Rhodiola: R. kirilowii and R. rosea. After collecting plants, aqueous and hydroalcoholic extracts were prepared. In order to identify analysed polyphenolic compounds ultra performance liquid chromatography - tandem mass spectrometry (UPLC-MS/MS, Waters) was used. Gallic acid is commonly found in the roots of these plants. Aqueous extract in both species is a rich source of gallic acid. The UPLC-MS/MS studies allow to use this analytical method for determination of polyphenolic acids accordance with the requirements of ICH. Chromatographic method developed by our team is more precise then previously published.

scholarly journals Cocktail Method: Effect of the Bletilla striata Extracts on CytochromeP450 Activity in Rat

2021 ◽  
Vol 16 (9) ◽  
pp. 1934578X2110324
Author(s):  
Yong Huang ◽  
Zu-Ying Zhou ◽  
Zi-Peng Gong ◽  
Yue-Ting Li ◽  
Si-Ying Chen ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Side Effects ◽  
Ultra Performance Liquid Chromatography ◽  
Pharmacokinetic Parameters ◽  
Tandem Mass ◽  
Control Groups ◽  
Bletilla Striata ◽  
Method Effect ◽  
Liquid Chromatography Tandem Mass ◽  
Different Doses

Bletilla striata is a dried tuber of B striata (Thunb.) Reichb.f. of Orchidaceae plant, which is mainly used for hemoptysis, vomiting blood, trauma bleeding, sore swollen poison, and cracked skin. There have been few research reports on the effect of this herb on cytochrome P450 (CYP), therefore, the study was aimed to investigate the effects of the B striata extracts on the activity of 6 subtypes (CYP2D6, 1A2, 2C19, 2E1, 3A4, and 2C9) using a cocktail method. The B striata extracts were administrated to rats in 0.21 or 0.63 g/kg once a day for 7 or 14 days. The 3 control groups were used to ensure the accuracy of the results. Subsequently, a cocktail of tolbutamide, chlorzoxazone, midazolam, metoprolol, omeprazole, and caffeine was injected. A ultra performance liquid chromatography–tandem mass spectrometer was developed and validated to investigate the concentration of the probes and the pharmacokinetic parameters were calculated to investigate the effects of the extracts on the activity of 6 enzymes under different doses and different dosing periods. The results suggested that the B striata extracts could induce the activities of CYP2D6, 1A2, and 2C19 and could inhibit the activities of CYP2E1, 3A4, and 2C9. When used in combination with drugs that are metabolized by CYP2D6, 1A2, 2C19, 2E1, 3A4, and 2C9, appropriate dose adjustments were needed to avoid toxic side effects caused by drug interactions.

scholarly journals Simultaneous Quantification of Five Flavanone Glycosides in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry: Application to a Comparative Pharmacokinetic Study of Aurantii Fructus Immaturus and Aurantii Fructus Extracts

2019 ◽  
Vol 102 (3) ◽  
pp. 781-787 ◽  
Author(s):  
Pei Li ◽  
Su-Ling Zeng ◽  
Zi-Yuan Wang ◽  
Qiang Yin ◽  
Zhi-Ming Bi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Pharmacokinetic Study ◽  
Plasma Concentrations ◽  
Ultra Performance Liquid Chromatography ◽  
Rat Plasma ◽  
Pharmacokinetic Parameters ◽  
Tandem Mass ◽  
Liquid Chromatography Tandem Mass ◽  
Health Promoting

Abstract Background: Aurantii Fructus Immaturus (AFI) and Aurantii Fructus (AF) are two traditional citrus herbs with health-promoting and nutritive properties. Objective: This paper presents the first attempt to simultaneously investigate the absorption of five major flavanone glycosides, namely narirutin, naringin, hesperidin, neohesperidin, and poncirin, in rat plasma following a single oral administration of AFI and AF extracts to rats. Methods: The plasma concentrations were determined by liquid–liquid extraction followed by a rapid and sensitive ultra-performance LC-tandem mass spectrometry method. Pharmacokinetic parameters were analyzed by noncompartmental modeling using DAS software. Results: The developed method was validated and successfully applied to the pharmacokinetic study of these five flavanone glycosides. Conclusions: The comparison of the pharmacokinetic parameters of flavanone glycosides showed that the absorption of AF extract was lower, while the elimination was relatively rapid, compared with those of AFI extract. Highlights: This study may be useful for further utilization of these two citrus herbs.

scholarly journals Effect of the Phragmitis Rhizoma Aqueous Extract on the Pharmacokinetics of Docetaxel in Rats

2019 ◽  
Vol 22 (5) ◽  
pp. 326-332
Author(s):  
Sarah Shin ◽  
No Soo Kim ◽  
Young Ah Kim ◽  
Hea Ry Oh ◽  
Ok-Sun Bang
Keyword(s):  
Aqueous Extract ◽  
Statistical Significance ◽  
Ultra Performance Liquid Chromatography ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
Sprague Dawley ◽  
Sprague Dawley Rats ◽  
Liquid Chromatography Tandem Mass ◽  
Mass Spectrometry System ◽  
Phragmitis Rhizoma

Background: Traditionally, Phragmitis rhizoma has been prescribed to relive a fever, vomiting, dysuria, and constipation, and to promote secretion of fluids. In addition, recent studies have reported its efficacy as a diuretic and antiemetic. Our previous study demonstrated that the Phragmitis rhizoma aqueous extract (EPR) ameliorates docetaxel (DTX)-induced myelotoxicity. Aim and Objective: This study was aimed to investigate the effects of EPR on the pharmacokinetics of DTX in Sprague–Dawley rats. Materials & Methods: The animals received an intravenous injection of DTX (5 mg/kg) with or without oral EPR (100 mg/kg) pretreatment for 1 or 6 days. The pharmacokinetics of plasma DTX was analyzed using an ultra-performance liquid chromatography-tandem mass spectrometry system, and pharmacokinetic parameters were estimated via noncompartmental analysis. Results: Relative to the control group (DTX alone), EPR pretreatment did not affect significantly the overall profiles of plasma DTX levels. Consecutively pretreated EPR for 6 days slightly altered AUC0-t and Cmax of DTX by 122 and 145.9%, respectively, but these data did not reach the threshold of statistical significance (p > 0.05). Conclusion: These results indicate that DTX exposure may not be affected by EPR treatment at the dose level used in this study, suggesting that oral EPR can be used safely when taken with intravenously injected DTX. However, further studies under the stringent conditions are needed when chronic treatment of EPR and anticancer drug.

scholarly journals The Effect of Posaconazole and Isavuconazole on the Pharmacokinetics of Erdafitinib in Beagle Dogs by UPLC-MS/MS

2021 ◽  
Vol 12 ◽  
Author(s):  
Lan-hong Ruan ◽  
Ling-ling Fan ◽  
Kun Wang ◽  
Wan-qi Zhang ◽  
Xiao-jun Wang ◽  
...  
Keyword(s):  
Ultra Performance Liquid Chromatography ◽  
Self Control ◽  
Pharmacokinetic Parameters ◽  
Beagle Dogs ◽  
Limit Of Quantification ◽  
Dog Plasma ◽  
Liquid Chromatography Tandem Mass ◽  
Group A ◽  
Group B ◽  
Second Period

Objective: A robust, quick, and reliable ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS) method for the quantification of erdafitinib in beagle dog plasma was developed and validated to evaluate the changes of posaconazole and isavuconazole on the pharmacokinetics of erdafitinib in beagle dogs, respectively.Methods: This experiment adopted a three-period self-control experimental design. In the first period (group A), erdafitinib was orally administered to six beagle dogs at a dose of 4 mg/kg. In the second period (group B), the same six beagle dogs were orally given posaconazole at a dose of 7 mg/kg, and after 30 min, erdafitinib was orally given. In the third period (group C), isavuconazole at a dose of 7 mg/kg was given orally, and then, erdafitinib was orally given. At the different time points after erdafitinib was given in the three periods, the blood samples were collected. The concentration of erdafitinib was detected by the developed UPLC-MS/MS method. DAS 2.0 was used to calculate the pharmacokinetic parameters of erdafitinib.Results: Erdafitinib had a good linear relationship in the range of 1–500 ng/ml, and the lower limit of quantification was 1 ng/ml. The precision, accuracy, extraction recovery, matrix effect, and stability meet the requirements of the guiding principles. After erdafitinib was combined with posaconazole, the Cmax and AUC0→t of erdafitinib increased by 27.19% and 47.62%, respectively, and the t1/2 was prolonged to 6.33 h. After erdafitinib was combined with isavuconazole, the Cmax and AUC0→t of erdafitinib increased by 23.13% and 54.46%, respectively, and the t1/2 was prolonged to 6.31 h.Conclusion: A robust and reliable UPLC-MS/MS method was fully optimized and developed to detect the plasma concentration of erdafitinib in beagle dogs. Posaconazole and isaconazole could inhibit the metabolism of erdafitinib in beagle dogs and increase the plasma exposure of erdafitinib.

scholarly journals Influences of Oldenlandia diffusa on the CYP450 Activities in Rats Using a Cocktail Method by UHPLC-MS/MS

2018 ◽  
Vol 2018 ◽  
pp. 1-6 ◽  
Author(s):  
Yiping Lin ◽  
Yanli Wei ◽  
Xiaoxia Hu ◽  
Meilling Wu ◽  
Xiaoqian Ying ◽  
...  
Keyword(s):  
Ultra Performance Liquid Chromatography ◽  
Metabolic Enzyme ◽  
Pharmacokinetic Parameters ◽  
Mass Spectrometry Detection ◽  
Chromatography Tandem Mass Spectrometry ◽  
Significant Difference ◽  
Liquid Chromatography Tandem Mass ◽  
Clinical Drug ◽  
Oldenlandia Diffusa

Oldenlandia diffusa has been used to treat various cancers. Cytochrome P450, a drug metabolic enzyme, might be influenced by herbal medicine. Currently, the problem that remains is the effective treatment in drug-drug interaction situation. Potential influences of Oldenlandia diffusa were elucidated on the CYP450 activities in rats using a cocktail method. Blood samples were precipitated by acetonitrile. Quantitative determination of target test object was done by ultra-performance liquid chromatography tandem mass spectrometry detection. Influences of oldenlandia diffusa on the activities of five CYP450 subtypes in rats were evaluated by five specific probe drugs (phenacetin for CYP1A2, omeprazole for CYP2C19, tolbutamide for CYP2C9, metoprolol for CYP2D6, and midazolam for CYP3A4) according to the pharmacokinetic parameters changes. No statistically significant difference (P>0.05) in pharmacokinetic behaviors can be observed in the five probe drugs. There is a potential guidance on clinical drug combination with Oldenlandia diffusa. Oldenlandia diffusa in compound preparation showed well security.

The Pharmacokinetics of Buserelin after Intramuscular Administration in Pigs and Cows

2021 ◽  
Author(s):  
Zhengrong Gao ◽  
Yu Liu ◽  
Yuxin Yang ◽  
Yuying Cao ◽  
Jicheng Qiu ◽  
...  
Keyword(s):  
Area Under The Curve ◽  
Apparent Volume ◽  
Ultra Performance Liquid Chromatography ◽  
Volume Of Distribution ◽  
Pharmacokinetic Parameters ◽  
Maximum Plasma ◽  
Elimination Half ◽  
Liquid Chromatography Tandem Mass ◽  
Stability Method ◽  
Apparent Volume Of Distribution

Abstract Background: Buserelin is a LHRH agonist used for the treatment of hormone-dependent diseases in males and females. However, the pharmacokinetics of buserelin in pigs and cows are not clearly understood. This study was designed to develop a sensitive method to determine the concentration of buserelin and to investigate the pharmacokinetic parameters after intramuscular (i.m.) administration in pigs and cows. Results: A sensitive and rapid stability method based on ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed. The pharmacokinetic parameters of buserelin after i.m. administration were studies in five pigs and five cows at a single dose of 1 mg per pig and 3 mg per cow. The plasma kinetics were analyzed by WinNonlin 8.1.0 software using a non-compartmental model. The mean concentration area under the curve (AUC0-t) was 25.02 ± 6.93 h·ng/mL for pigs and 5.63 ±1.86 h·ng/mL for cows. The maximum plasma concentration (Cmax) and time to reach the maximum concentration (tmax) were 10.99 ± 2.04 ng/mL and 0.57 ± 0.18 h for pigs and 2.68 ± 0.36 ng/mL and 1.05 ±0.27 h for cows, respectively. The apparent volume of distribution (Vz) in pigs and cows was 80.49 ± 43.88 L and 839.88 ± 174.77 L, respectively. The elimination half-time (t1/2λz), and clearance (CL) were 1.29 ± 0.40 h and 41.15 ± 11.18 L/h for pigs and 1.13 ± 0.3 h and 545.04 ± 166.40 L/h for cows, respectively. No adverse effects were observed in any of the animals. Conclusion: This study extends previous studies describing the pharmacokinetics of buserelin following i.m. administration in pigs and cows. Further studies investigating other factors were needed to establish therapeutic protocol in pigs and cows and to extrapolate these parameters to others economic animals.

scholarly journals Extraction and Identification of Bioactive Compounds from L. pumila

2019 ◽  
Vol 3 (3) ◽  
pp. 1-5
Author(s):  
Keyword(s):  
Gallic Acid ◽  
Bioactive Compounds ◽  
Protocatechuic Acid ◽  
Extraction Methods ◽  
Ultra Performance Liquid Chromatography ◽  
Extraction Yield ◽  
Exact Match ◽  
External Standard ◽  
Ultrasonic Assisted ◽  
Photodiode Array

This paper presents the comparison of extraction methods between maceration extraction (ME) and ultrasonic assisted extarction (UAE) to the bioactive compounds yield (gallic acid, protocatechuic acid, epigallocatechin and rutin) of Labisia pumila. A grinded dried plant material with size ranging 246.58μm to 257.72μm was performed throughout this work. The gallic acid, protoctechuic acid, epigallocatechin and rutin qualification and quantification were performed using an ultra-performance liquid chromatography coupled photodiode array (UPLC-PDA). Exact match between the residence time from the plant extract and external standard was found indicating a presence of these four targeted bioactive compounds. It was found that UAE method has the highest extraction yield; gallic acid (0.0293 mg GA/g DW), protocatechuic acid (0.0081 mg PCA/g DW), epigallocatechin (0.0057 mg EGC/g DW) and rutin (0.0038 mg Rutin/g DW) compared to ME. The findings in this work may serve as a useful guide to obtain a highest extraction yield of these four targeted bioactive compounds from L. pumila.

scholarly journals Improving Bioavailability of Gallic Acid and Quercetin by Designing Polymeric Nanoparticle Formulation

2021 ◽  
Author(s):  
poournima shamrao Patil ◽  
Suresh Ganapati killedar
Keyword(s):  
Gallic Acid ◽  
Polymeric Nanoparticles ◽  
Fold Increase ◽  
Pharmacokinetic Parameters ◽  
Pomegranate Peel ◽  
Polymeric Nanoparticle ◽  
Pharmacokinetic Properties ◽  
Pomegranate Peel Extract ◽  
Pomegranate Fruit ◽  
Work Method

Abstract Objective The pharmacokinetic properties of encapsulated polyherbal nanoparticles (Gallic acid and quercetin nanoparticle) and polyherbal extract (Amla and pomegranate fruit peels) in normal and DMH-induced colorectal cancer in rats was examined in this work. Method Polyherbal nanoparticles such as Gallic acid isolated from amla fruit and quercetin from pomegranate peel extract formulated into polymeric nanoparticle by using probe sonicator and high pressure homogenization. Result In normal and DMH-induced rats, a pharmacokinetic study demonstrated that polyherbal nanoparticles had a typical sustained release profile with a 2-fold increase in bioavailability when compared to polyherbal extract. Based on serum–concentration profiles of polyherbal nanoparticles and polyherbal extract following oral administration, the pharmacokinetic parameters for polyherbal nanoparticles and polyherbal extract were established using a single compartmental approach. Conclusion This research suggests that encapsulating gallic acid and quercetin in polymeric nanoparticles improves oral bioavailability than polyherbal extract of amla and pomegranate peel. Polymeric nanoparticles could be a novel therapeutic possibility for carcinogenesis prevention.

Simultaneous Determination of Three Alkaloids in Wutou Decoction in Rat Plasma Via the UPLC–MS/MS Method and its Application in Pharmacokinetic Study

2020 ◽  
Vol 16 (5) ◽  
pp. 558-563
Author(s):  
WU Jian ◽  
JI Yu-bin ◽  
Liu Ying-jie ◽  
XU Ying ◽  
Zheng Wei ◽  
...  
Keyword(s):  
Electrospray Ionization ◽  
Pharmacokinetic Study ◽  
Multiple Reaction Monitoring ◽  
Ultra Performance Liquid Chromatography ◽  
Rat Plasma ◽  
Pharmacokinetic Parameters ◽  
Intragastric Administration ◽  
Highly Sensitive ◽  
Pharmacokinetic Properties

Introduction: Wutou decoction has been wildly applied for the treatment of RA in China for several thousand years. Methods: This study aims to develop a highly sensitive and specific ultra performance liquid chromatography coupled with tandem mass spectrometry and electrospray ionization (UPLC-ESI-MS/MS) method to explore the pharmacokinetic properties of three representative bioactive alkaloids after intragastric administration of Wutou decoction in rats. Chromatographic separation was performed on a C18 column under the Multiple Reaction Monitoring (MRM) in the positive electrospray ionization (ESI) mode. The pharmacokinetic parameters were evaluated by software DAS 3. 0. Results: The validation of the method was achieved in accordance with the FDA guidelines. The results of pharmacokinetic study showed that the in vivo concentrations of benzoylmesaconine and benzoylhypaconine were significantly higher than benzoylaconine. Our PK results showed that these three compounds were quickly absorbed after the administration of Wutou decoction, and Tmax ranged from 30 min to 45 min. Conclusion: The results of pharmacokinetic study showed that the in vivo concentrations of benzoylmesaconine and benzoylhypaconine were significantly higher than benzoylaconine. There were also similar pharmacokinetic behaviors observed among BAC, BHA, and BMA after oral administration of WTD.

Sign in / Sign up

Export Citation Format

Share Document