Surface Permeability of Membrane and Catalytic Performance Based on Redox-Responsive of Hybrid Hollow Polymeric Microcapsules

“Smart” polymeric microcapsules with excellent permeability of membranes have drawn considerable attention in scientific and industrial research such as drug delivery carriers, microreactors, and artificial organelles. In this work, hybrid hollow polymeric microcapsules (HPs) containing redox-active gold-sulfide bond were prepared with bovine serum albumin, inorganic metal cluster (AuNCs), and poly(N-isopropylacrylamide) conjugates by using Pickering emulsion method. HPs were transferred from water-in-oil to water-in-water by adding PEGbis(N-succinimidylsuccinate). To achieve redox-responsive membrane, the Au-S bond units incorporated into the microcapsules’ membranes, allowed us to explore the effects of a new stimuli, that is, the redox Au-S bond breaking on the microcapsules’ membranes. The permeability of these hybrid hollow polymeric microcapsules could be sensitively tuned via adding environment-friendly hydrogen peroxide (H2O2), resulting from a fast fracture of Au-S bond. Meanwhile, AuNCs and conjugates could depart from the microcapsules, and enhance the permeability of the membrane. Based on the excellent permeability of the membrane, phosphatase was encapsuled into HPs and p-nitrophenyl phosphate as a substrate. After adding 1 × 10−2 and 1 × 10−4 M H2O2, the catalytic efficiency was nearly 4.06 and 2.22 times higher than that of HPs in the absence of H2O2, respectively. Hence, the unique redox-responsive HPs have potential applications in biocatalytic reaction, drug delivery, and materials as well as in bioscience.

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Mesoporous bimetallic Fe/Co as highly active heterogeneous Fenton catalyst for the degradation of tetracycline hydrochlorides

Scientific Reports ◽

10.1038/s41598-019-52013-y ◽

2019 ◽

Vol 9 (1) ◽

Cited By ~ 1

Author(s):

Junchao Li ◽

Xuhua Li ◽

Jindong Han ◽

Fansheng Meng ◽

Jinyuan Jiang ◽

...

Keyword(s):

Bimetallic Catalyst ◽

Removal Rate ◽

Catalytic Efficiency ◽

Nitrogen Adsorption ◽

Catalytic Performance ◽

Operating Parameters ◽

Co Catalyst ◽

Highly Active ◽

Fenton Catalyst ◽

Potential Applications

Abstract Mesoporous bimetallic Fe/Co was prepared as a Fenton-like catalyst to degrade the tetracycline hydrochlorides (TC). The nanocasting strategy with KIT-6 as a hard template was carried out to synthesize the mesoporous bimetallic catalyst. The mesoporous bimetallic Fe/Co catalyst was characterized by X-ray diffraction (XRD), transmission electron microscopy (TEM), nitrogen adsorption-desorption isotherms, and Brunauer-Emmett-Teller (BET) method. The results showed that the catalyst has significant nanofeatures; the surface area, pore size, and particle size were 113.8 m2g−1, 4 nm, and 10 nm, respectively. In addition, the effects of the operating parameters, such as the iron-to-cobalt ratio, pH, H2O2, and initial TC concentrations on its catalytic performance were investigated. The best operating parameters were as follows: iron-to-cobalt ratio = 2:1 to 1:1, pH = 5–9, H2O2: 30 mmol, initial TC less than 30 mg/L. Furthermore, the mesoporous bimetallic Fe/Co showed a good performance for degrading TC, achieving a removal rate of 86% of TC after 3 h under the reaction conditions of H2O2 = 30 mmol, mesoporous bimetallic Fe/Co = 0.6 g/L, TC = 30 mg/L, pH = 7.0, and temperature = 25.5 °C. The mesoporous bimetallic Fe/Co catalyst shows good stability and reusability. This work demonstrated that mesoporous bimetallic Fe/Co has excellent catalytic efficiency, smaller amounts of leached ions, and wider pH range, which enhance its potential applications.

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Tailoring Pullulanase PulAR from Anoxybacillus sp. AR-29 for Enhanced Catalytic Performance by A Structure-Guided Consensus Approach

10.21203/rs.3.rs-1189094/v1 ◽

2022 ◽

Author(s):

Shu-Fang Li ◽

Shen-Yuan Xu ◽

Ya-Jun Wang ◽

Yu-Guo Zheng

Keyword(s):

Catalytic Efficiency ◽

Catalytic Performance ◽

Site Directed Mutagenesis ◽

Wild Type ◽

Consensus Approach ◽

Debranching Enzyme ◽

Rational Engineering ◽

Potential Applications ◽

Quadruple Mutant ◽

Starch Industry

Abstract Pullulanase is a well-known debranching enzyme that can specially hydrolyze α-1,6-glycosidic linkages in starch and oligosaccharides, however, it suffers from low stability and catalytic efficiency under industrial conditions. In the present study, four sites (A365, V401, H499, and T504) lining the catalytic pocket of Anoxybacillus sp. AR-29 pullulanase PulAR were selected for site-directed mutagenesis (SDM) by using a structure-guided consensus approach. Four beneficial mutants (PulAR-A365V, PulAR-V401C, PulAR-A365/V401C, PulAR-A365V/V401C/T504V, and PulAR-A365V/V401C/T504V/H499A) were created, which showed enhanced thermostability, pH stability, and catalytic efficiency. Among them, the quadruple mutant PulAR-A365V/V401C/T504V/H499A displayed 6.6- and 9.6-fold higher catalytic efficiency toward pullulan at 60 ℃, pH 5.0 and 6.0, respectively. In addition, its thermostabilities at 60 ℃ and 65 ℃ were improved by 2.6- and 3.1-fold, respectively, compared to those of the wild-type (WT). Meanwhile, its pH stabilities at pH 4.5 and 5.0 were 1.6- and 1.8-fold higher than those of WT, respectively. In summary, the catalytic performance of PulAR was significantly enhanced via rational engineering by a structure-guided consensus approach. The resultant quadruple mutant PulAR-A365V/V401C/T504V/H499A demonstrated potential applications in the starch industry.

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2,2′:6′,2′′-Terpyridine-functionalized redox-responsive hydrogels as a platform for multi responsive amphiphilic polymer membranes

RSC Advances ◽

10.1039/c6ra23677d ◽

2016 ◽

Vol 6 (100) ◽

pp. 97921-97930 ◽

Cited By ~ 6

Author(s):

Katrin Schöller ◽

Claudio Toncelli ◽

Juliette Experton ◽

Susanne Widmer ◽

Daniel Rentsch ◽

...

Keyword(s):

Drug Delivery ◽

Polymer Membranes ◽

Amphiphilic Polymer ◽

Redox Responsive ◽

Medical Sensors ◽

Responsive Membranes ◽

Potential Applications ◽

Responsive Hydrogels

Amphiphilic polymer co-networks were functionalized with spyropiran and terpyridine yielding multi-responsive membranes with switchable properties and potential applications in drug delivery and medical sensors.

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An Update on Recent Advances in Cubosome: A Novel Drug Delivery System

Current Drug Metabolism ◽

10.2174/1389200221666210105121532 ◽

2021 ◽

Vol 21 ◽

Author(s):

Madhukar Garg ◽

Anju Goyal ◽

Sapna Kumari

Keyword(s):

Drug Delivery ◽

Drug Delivery System ◽

Lipid Bilayers ◽

Delivery System ◽

Liquid Crystalline ◽

Three Dimensional ◽

Biologically Active ◽

Potential Applications ◽

Health Related ◽

Novel Drug

: Cubosomes are highly stable nanostructured liquid crystalline dosage delivery form derived from amphiphilic lipids and polymer-based stabilizers converting it in a form of effective biocompatible carrier for the drug delivery. The delivery form comprised of bicontinuous lipid bilayers arranged in three dimensional honeycombs like structure provided with two internal aqueous channels for incorporation of number of biologically active ingredients. In contrast liposomes they provide large surface area for incorporation of different types of ingredients. Due to the distinct advantages of biocompatibility and thermodynamic stability, cubosomes have remained the first preference as method of choice in the sustained release, controlled release and targeted release dosage forms as new drug delivery system for the better release of the drugs. As lot of advancement in the new form of dosage form has bring the novel avenues in drug delivery mechanisms so it was matter of worth to compile the latest updates on the various aspects of mentioned therapeutic delivery system including its structure, routes of applications along with the potential applications to encapsulate variety drugs to serve health related benefits.

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Focused ultrasound in neuro-oncology: the role of the Focused Ultrasound Foundation in driving adoption and innovation

Journal of Neuro-Oncology ◽

10.1007/s11060-021-03808-5 ◽

2021 ◽

Author(s):

Emily C. Whipple ◽

Camille A. Favero ◽

Neal F. Kassell

Keyword(s):

Drug Delivery ◽

Brain Tumors ◽

Focused Ultrasound ◽

Clinical Evidence ◽

Neurological Injury ◽

High Concentration ◽

Potential Applications ◽

Tumor Agent

Abstract Introduction Intra-arterial (lA) delivery of therapeutic agents across the blood-brain barrier (BBB) is an evolving strategy which enables the distribution of high concentration therapeutics through a targeted vascular territory, while potentially limiting systemic toxicity. Studies have demonstrated lA methods to be safe and efficacious for a variety of therapeutics. However, further characterization of the clinical efficacy of lA therapy for the treatment of brain tumors and refinement of its potential applications are necessary. Methods We have reviewed the preclinical and clinical evidence supporting superselective intraarterial cerebral infusion (SSJACI) with BBB disruption for the treatment of brain tumors. In addition, we review ongoing clinical trials expanding the applicability and investigating the efficacy of lA therapy for the treatment of brain tumors. Results Trends in recent studies have embraced the use of SSIACI and less neurotoxic chemotherapies. The majority of trials continue to use mannitol as the preferred method of hyperosmolar BBB disruption. Recent preclinical and preliminary human investigations into the lA delivery of Bevacizumab have demonstrated its safety and efficacy as an anti-tumor agent both alone and in combination with chemotherapy. Conclusion lA drug delivery may significantly affect the way treatment are delivered to patients with brain tumors, and in particular GBM. With refinement and standardization of the techniques of lA drug delivery, improved drug selection and formulations, and the development of methods to minimize treatment-related neurological injury, lA therapy may offer significant benefits for the treatment of brain tumors.

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In vivo interactome profiling by enzyme‐catalyzed proximity labeling

Cell & Bioscience ◽

10.1186/s13578-021-00542-3 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Yangfan Xu ◽

Xianqun Fan ◽

Yang Hu

Keyword(s):

State Of The Art ◽

Catalytic Efficiency ◽

Biological Processes ◽

Protein Protein Interaction ◽

Current State ◽

Protein Interactome ◽

Potential Applications ◽

Protein Protein Interaction Networks ◽

Temporal And Spatial

AbstractEnzyme-catalyzed proximity labeling (PL) combined with mass spectrometry (MS) has emerged as a revolutionary approach to reveal the protein-protein interaction networks, dissect complex biological processes, and characterize the subcellular proteome in a more physiological setting than before. The enzymatic tags are being upgraded to improve temporal and spatial resolution and obtain faster catalytic dynamics and higher catalytic efficiency. In vivo application of PL integrated with other state of the art techniques has recently been adapted in live animals and plants, allowing questions to be addressed that were previously inaccessible. It is timely to summarize the current state of PL-dependent interactome studies and their potential applications. We will focus on in vivo uses of newer versions of PL and highlight critical considerations for successful in vivo PL experiments that will provide novel insights into the protein interactome in the context of human diseases.

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Metal-Organic Framework-Based Engineered Materials—Fundamentals and Applications

Molecules ◽

10.3390/molecules25071598 ◽

2020 ◽

Vol 25 (7) ◽

pp. 1598 ◽

Cited By ~ 5

Author(s):

Tahir Rasheed ◽

Komal Rizwan ◽

Muhammad Bilal ◽

Hafiz M. N. Iqbal

Keyword(s):

Drug Delivery ◽

Metal Organic Framework ◽

High Surface ◽

High Surface Area ◽

Co2 Reduction ◽

Biological Species ◽

Crystalline Materials ◽

Potential Applications ◽

Metal Organic ◽

Catalytic Chemistry

Metal-organic frameworks (MOFs) are a fascinating class of porous crystalline materials constructed by organic ligands and inorganic connectors. Owing to their noteworthy catalytic chemistry, and matching or compatible coordination with numerous materials, MOFs offer potential applications in diverse fields such as catalysis, proton conduction, gas storage, drug delivery, sensing, separation and other related biotechnological and biomedical applications. Moreover, their designable structural topologies, high surface area, ultrahigh porosity, and tunable functionalities all make them excellent materials of interests for nanoscale applications. Herein, an effort has been to summarize the current advancement of MOF-based materials (i.e., pristine MOFs, MOF derivatives, or MOF composites) for electrocatalysis, photocatalysis, and biocatalysis. In the first part, we discussed the electrocatalytic behavior of various MOFs, such as oxidation and reduction candidates for different types of chemical reactions. The second section emphasizes on the photocatalytic performance of various MOFs as potential candidates for light-driven reactions, including photocatalytic degradation of various contaminants, CO2 reduction, and water splitting. Applications of MOFs-based porous materials in the biomedical sector, such as drug delivery, sensing and biosensing, antibacterial agents, and biomimetic systems for various biological species is discussed in the third part. Finally, the concluding points, challenges, and future prospects regarding MOFs or MOF-based materials for catalytic applications are also highlighted.

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Mesoporous Silica Nanoparticles: Their Projection in Nanomedicine

ISRN Materials Science ◽

10.5402/2012/608548 ◽

2012 ◽

Vol 2012 ◽

pp. 1-20 ◽

Cited By ~ 26

Author(s):

María Vallet-Regí

Keyword(s):

Drug Delivery ◽

Mesoporous Silica ◽

Silica Nanoparticles ◽

Mesoporous Silica Nanoparticles ◽

Surrounding Medium ◽

Stimuli Responsive ◽

Guest Molecules ◽

Different Types ◽

Potential Applications ◽

Inorganic Nanomaterials

Mesoporous silica nanoparticles are receiving growing attention by the scientific biomedical community. Among the different types of inorganic nanomaterials, mesoporous silica nanoparticles have emerged as promising multifunctional platforms for nanomedicine. Since their introduction in the drug delivery landscape in 2001, mesoporous materials for drug delivery are receiving growing scientific interest for their potential applications in the biotechnology and nanomedicine fields. The ceramic matrix efficiently protects entrapped guest molecules against enzymatic degradation or denaturation induced by pH and temperature as no swelling or porosity changes take place as a response to variations in the surrounding medium. It is possible to load huge amounts of cargo into the mesopore voids and capping the pore entrances with different nanogates. The application of a stimulus provokes the nanocap removal and triggers the departure of the cargo. This strategy permits the design of stimuli-responsive drug delivery nanodevices.

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