Wood Extractives of Silver Fir and Their Antioxidant and Antifungal Properties

The chemical composition of extractives in the sapwood (SW), heartwood (HW), knotwood (KW), and branchwood (BW of silver fir (Abies alba Mill.) was analyzed, and their antifungal and antioxidant properties were studied. In addition, the variability of extractives content in a centripetal direction, i.e., from the periphery of the stem towards the pith, was investigated. The extracts were analyzed chemically with gravimetry, spectrophotometry, and chromatography. The antifungal and antioxidative properties of the extracts were evaluated by the agar well diffusion method and the diphenyl picrylhydrazyl radical scavenging method. Average amounts of hydrophilic extractives were higher in KW (up to 210.4 mg/g) and BW (148.6 mg/g) than in HW (34.1 mg/g) and SW (14.8 mg/g). Extractives identified included lignans (isolariciresinol, lariciresinol, secoisolariciresinol, pinoresinol, matairesinol) phenolic acids (homovanillic acid, coumaric acid, ferulic acid), and flavonoids epicatechin, taxifolin, quercetin). Secoisolariciresinol was confirmed to be the predominant compound in the KW (29.8 mg/g) and BW (37.6 mg/g) extracts. The largest amount of phenolic compounds was extracted from parts of knots (281.7 mg/g) embedded in the sapwood and from parts of branches (258.9 mg/g) adjacent to the stem. HW contained more lignans in its older sections. Hydrophilic extracts from knots and branches inhibited the growth of wood-decaying fungi and molds. KW and BW extracts were better free radical scavengers than HW extracts. The results of the biological activity tests suggest that the protective function of phenolic extracts in silver fir wood can also be explained by their antioxidative properties. The results of this study describe BW as a potential source of phenolic extractives in silver fir.

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Ultrasound-assisted extraction of protein from Bombay locusts and its impact on functional and antioxidative properties

Scientific Reports ◽

10.1038/s41598-021-96694-w ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Passakorn Kingwascharapong ◽

Manat Chaijan ◽

Supatra Karnjanapratum

Keyword(s):

Antioxidant Properties ◽

Radical Scavenging ◽

Surface Hydrophobicity ◽

Molecular Characteristics ◽

Ultrasound Assisted Extraction ◽

Antioxidative Properties ◽

Assisted Extraction ◽

Ultrasound Assisted ◽

Nonessential Amino Acids ◽

The Impact

AbstractImpact of ultrasound-assisted process (UAP) on yield, functional properties, antioxidant properties and molecular characteristics of protein extracted from Bombay locusts (BL) (Patanga succinta L.) was studied. Different conditions of UAP were implemented for different amplitudes (40–60%) and times (10–30 min) during aqueous extraction. Notably, UAP could enhance yield and protein recovery, compared with those from typical process (TP) (continuously stirred at 100 rpm at room temperature for 1 h). UAP conditions used governed the change of surface hydrophobicity and free α-amino content of BL. UAP could improve solubility of BL, especially at pH levels higher than 2. UAP had no significant (p > 0.05) detrimental effects on foaming capacity and stability of BL. Nevertheless, UAP, particularly at 50–60% amplitudes, affected the emulsion activity and stability of BL. UAP provided BL with high radical scavenging activities and good electron donating ability, especially that from 60% amplitude for 20 min (UAP-60/20). UAP-60/20 showed the impact on change of isoelectric point and molecular characteristic monitored by Fourier transform infrared (FTIR) of BL, compared to those from TP. In addition, BL was also an excellent source of both essential and nonessential amino acids. Therefore, UAP potentially enhanced BL extraction efficiency, resulting the BL with good functional and antioxidative properties.

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Aktywność przeciwutleniająca i przeciwbakteryjna miodu, propolisu oraz pyłku kwiatowego

Postępy Fitoterapii ◽

10.25121/pf.2020.21.3.123 ◽

2020 ◽

Vol 21 (3) ◽

Author(s):

Magdalena Woźniak ◽

Lucyna Mrówczyńska ◽

Anna Sip ◽

Marta Babicka ◽

Tomasz Rogoziński ◽

...

Keyword(s):

Antioxidant Activity ◽

Phenolic Compounds ◽

Pathogenic Bacteria ◽

Radical Scavenging Activity ◽

Colorimetric Method ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Total Content ◽

Diffusion Method ◽

Bacterial Strains

Introduction. Honey, propolis and pollen belong to bee products that have beneficial biological properties. These products exhibit e.g. antibacterial, antifungal and antioxidant properties. Due to biological activity and natural origin, bee products are used, e.g. in the food industry, cosmetology and pharmacy. Aim. The aim of the study was to compare the antioxidant and antibacterial activity of honey, propolis and pollen from an apiary located in Wielkopolska Province. Material and methods. Honey, propolis and pollen used in this study came from the same apiary located in Wielkopolska Province. The antioxidant potential of bee products was evaluated applying DPPH· free radical scavenging activity assay. The antimicrobial activity of the tested bee products was determined by the point-diffusion method against 13 strains of pathogenic and potentially pathogenic bacteria. In addition, the total content of phenolic compounds in honey, propolis and pollen was determined by the colorimetric method. Results. Propolis exhibited higher antioxidant activity, in comparison to honey and pollen. The antiradical activity of propolis was equal to 80% approx. activity of Trolox, the standard antioxidant. Among tested bee products, propolis was characterized by the highest total phenols content. In addition, honey, propolis and pollen showed antagonistic activity against tested bacterial strains. Conclusions. The obtained results indicate that among the tested bee products of native origin, i.e. honey, propolis and pollen, propolis characterized by the highest antioxidant activity and the total content of phenolic compounds. In addition, all bee products showed bactericidal activity against the tested bacterial strains.

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ANTIOXIDANT AND ANTIMICROBIAL PROPERTIES OF Tinospora crispa (putarwali) STEMS METHANOLIC EXTRACT

Jurnal Teknologi ◽

10.11113/jt.v78.9941 ◽

2016 ◽

Vol 78 (11-2) ◽

Cited By ~ 1

Author(s):

Fatimatul Akmal Sulaiman ◽

Nurfarahin Fuad ◽

Farawahida Rahman ◽

Anwar Iqbal ◽

Deny Susanti Darnis

Keyword(s):

Antimicrobial Agents ◽

Methanolic Extract ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Antimicrobial Properties ◽

Diffusion Method ◽

Disc Diffusion ◽

Disc Diffusion Method ◽

Average Percentage ◽

Dried Extract

Apart from being the primary source of food to other living things, plants also have medicinal value to treat various kinds of diseases. In recent years, it has been proposed that the extract from plants may be used as natural antioxidants which can help to prevent the generation of carcinogens in human body. In addition, plants also have antimicrobial agents to inhibit the growth of pathogenic microbes. This study was intended to investigate the antioxidant properties and antimicrobial activity of methanolic extract of Tinospora crispa stems extracted using soxhlet extraction method. The antimicrobial properties of T. crispa stems extract were tested using disc diffusion method against Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778, Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Candida albicans IMR C S23/11 A and Saccharomyces cerevisiae IMR S 617/068. The antioxidant properties of the extract were investigated by using Total Phenolics Content (TPC), Total Flavonoids Content (TFC), DPPH free radical scavenging and b–carotene bleaching assays. The TPC value was 6.12 g GAE/100 g of dried extract while the TFC value was 55.58 g QE/100 g of dried extract. The IC50 of DPPH scavenging assay for the extract and ascorbic acid were 0.21 and 0.04 mg/mL, respectively. The average percentage of b–carotene bleaching assay was 38.3 % as compared to BHT, which was 45.1%. The disc diffusion method showed no inhibition zone against all the strains of microorganisms at all concentrations of the extracts (0.5, 1.0, 2.5 and 5.0 mg/disc).

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PHYTOCHEMICAL ANALYSIS AND ANTIBACTERIAL AND ANTIOXIDANT PROPERTIES OF INDIGOFERA TINCTORIA L.

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2018.v11i6.20060 ◽

2018 ◽

Vol 11 (6) ◽

pp. 136

Author(s):

Chandrasekaran Swaminathan

Keyword(s):

Antioxidant Activity ◽

Free Radical ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Diffusion Method ◽

Phytochemical Analysis ◽

Leaf Extracts ◽

Root Extracts ◽

Wide Range ◽

Indigofera Tinctoria

Objective: The present study was designed to evaluate the phytochemical composition and antibacterial and antioxidant potential of methanolic leaf and root extracts of Indigofera tinctoria L.Methods: Phytochemical analysis was done using standard methods. The methanolic leaf and root extracts of the plant were tested against Staphylococcus aureus, Enterococcus faecalis, Klebsiella pneumoniae, Enterobacter aerogenes, and Salmonella paratyphi B by cup-plate agar diffusion method. The free radical scavenging activities of the methanol extracts of leaves and roots were evaluated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay.Results: Phytochemical screening revealed the presence of carbohydrates, reducing sugars, alkaloids, saponins, phenolic compounds, and flavonoids in methanolic leaf and root extracts. Methanolic leaf extracts of the plant exhibited antibacterial activity against a wide range of bacteria, but the root extracts failed to inhibit the tested bacterial pathogens. The antioxidant activity determination revealed that at 100 μg/ml, methanolic root extracts had the highest antioxidant activity (89.10%) on DPPH free radicals followed by methanolic leaf extracts (46.74%).Conclusion: The results of the present study conclude that the studied plant possesses broad-spectrum antibacterial and antioxidant properties and may act as a potent antioxidant for biological systems susceptible to free radical-mediated reactions.

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ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF RUTA ANGUSTIFOLIA EXTRACT

Jurnal Teknologi ◽

10.11113/jt.v77.6747 ◽

2015 ◽

Vol 77 (25) ◽

Cited By ~ 1

Author(s):

Nur Azfa Shuib ◽

Anwar Iqbal ◽

Fatimatul Akmal Sulaiman ◽

Izzatie Razak ◽

Deny Susanti

Keyword(s):

Antibacterial Activity ◽

Phenolic Content ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Diffusion Method ◽

Total Phenolic ◽

Disc Diffusion ◽

Flavonoid Content ◽

Disc Diffusion Method ◽

Polyphenolic Content

Ruta angustifolia was used in this study in order to evaluate the antimicrobial activity and antioxidant properties and its correlation with the polyphenolic content. Two Gram-positive bacteria (Staphylococcus aureus ATCC 25923, Bacillus cereus ATCC 11778) and two Gram–negative bacteria (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 8739) were used to determine the antibacterial activity. Aqueous maceration extract was used for antioxidant activities and methanolic maceration extract was used for antibacterial activity. The antioxidant properties and activities were evaluated by using total phenolic content (TPC), total flavonoid content (TFC), DPPH free radical scavenging activity and beta-carotene bleaching method. Whereas, the antibacterial activity was examined using disc diffusion method against selected microorganism at concentration 1.0 mg/disc. The results showed the phenolic content of R. angustifolia extract was 18.89 g GAE/100 g extract while the flavonoid content was 14.170 g QE/100 g extract. R. angustifolia exhibited good radical scavenging with IC50 value of 2.04 mg/ml. The result for disc diffusion method showed no inhibition zone against all the strains of bacteria at 1.0 mg/disc concentration of the extract. Based on the results, it can be concluded that the R. angustifolia aqueous extract has the antioxidant properties and there is correlation between polyphenolic content of the extract with its antioxidant activity. However, R. angustifolia methanolic extract did not show any antibacterial activity.

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Polyphenolic Contents and Antioxidant Properties of Different Grape (V. vinifera,V. labrusca, andV. hybrid) Cultivars

BioMed Research International ◽

10.1155/2013/718065 ◽

2013 ◽

Vol 2013 ◽

pp. 1-5 ◽

Cited By ~ 26

Author(s):

Shivraj Hariram Nile ◽

S. H. Kim ◽

Eun Young Ko ◽

Se Won Park

Keyword(s):

Antioxidant Activities ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Total Phenolic ◽

Coumaric Acid ◽

Grape Skin ◽

Phenolic Contents ◽

Grape Skins ◽

Phenolic And Flavonoid

The polyphenolic contents and the antioxidant activity of the skins and pulps of different grape cultivars were estimated using HPLC and DPPH antioxidant assay, respectively. The phenolics and flavonoids identified were quercetin, kaempferol, caffeic acid, p-coumaric acid, cinnamic acid, and (−)-epicatechin. The total phenolic contents were found to be the highest in the grape skin of Flouxa (>400 mg/100 g), followed by Campbell Early and Tamnara (>300 mg/100 g), and then by Red Globe and Ruby Seedless (>250 mg/100 g), and the total phenolic content was the lowest in Italia and Delaware (<60 mg/100 g). The antioxidant activities of the grape extracts varied from 12.5% (Ruby Seedless) to 60.2% (Hongiseul) for skins, whereas the antioxidant activities of the grape extracts varied from 35.4% (Campbell Early) to 84.5% (Hongiseul) for pulps. The grape pulps have stronger antioxidant activities than those of the grape skins. Our results suggest that the phenolic and flavonoid contents in extracts of grape skins and pulps showed statistically significant correlations with the free radical scavenging activity.

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Effect of different extraction solvents on antioxidant properties of water lily (Nymphaeaceae antares) flower petal and stamen

Food Research ◽

10.26656/fr.2017.5(3).653 ◽

2021 ◽

Vol 5 (3) ◽

pp. 359-368

Author(s):

Z. Mohd Zin ◽

A.Z.M. Sanuri ◽

N. Bashah ◽

K. Ibrahim ◽

H. Yahya ◽

...

Keyword(s):

Phenolic Compounds ◽

Ethyl Acetate ◽

Ethyl Acetate Extract ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Total Phenolic ◽

Scavenging Activity ◽

Water Lily ◽

Antioxidative Properties ◽

Extraction Solvents

Water lily (Nymphaeaceae antares) contains a vast array of phenolic compounds with important natural antioxidative properties that could inhibit the adverse effects of the reactive oxygen species produced in living things. These substances are complex in composition which requires a suitable extraction medium to exploit the phenolic compounds. This study was designed to determine the antioxidative properties in petal and stamen of water lily extracted using different extraction solvents namely ethyl acetate and 60% methanol. Antioxidant properties of the samples were determined using 2,2-diphenyl -1-picrylhydrazyl (DPPH) radical scavenging activity, ferric thiocyanate (FTC) method, thiobarbituric acid (TBA) test, total phenolic content (TPC), total flavonoid content (TFC) and total tannin content (TTC) along with individual flavonoids content using High Performance Liquid Chromatography (HPLC). The yield of extract was found to be the highest in petals extracted with 60% methanol (44.55±1.63%) while stamen extracted with ethyl acetate yielded the lowest (24.50±1.09%). In the DPPH inhibition method, petal extracted with ethyl acetate obtained the highest scavenging activity (89.15±3.62%). FTC analysis revealed that ethyl acetate extract of petal experienced the highest percentage inhibition (76.70±6.48%) while in TBA analysis ethyl acetate extract of stamen exhibited the highest percentage inhibition (70.78±1.39%). Flavonoids such as quercetin, kaempferol, rutin, gallic acid, catechin, epigallocatechin, p-coumaric acid and myricetin were found to be present in ethyl acetate and 60% methanol extracts of water lilies stamen and petal. The information from this study indicates that water lily petals and stamens may be integrated as a good source of nutrients in the food industry, the pharmaceutical industry. If the advantages of water lily are not used and integrated into everyday life for health benefits, it will be a massive waste of natural resources.

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Chinese bayberry Jiaosu fermentation – changes of mycobiota composition and antioxidant properties

International Journal of Food Engineering ◽

10.1515/ijfe-2020-0238 ◽

2021 ◽

Vol 0 (0) ◽

Author(s):

Sheng Fang ◽

Zhening Jin ◽

Yisong Xu ◽

Ruyi Sha ◽

Jianwei Mao ◽

...

Keyword(s):

Saccharomyces Cerevisiae ◽

Phase 1 ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Phase 2 ◽

Dynamic Changes ◽

Total Polyphenols ◽

Chinese Bayberry ◽

Antioxidative Properties ◽

Incertae Sedis

Abstract The aim of the study was to investigate the dynamic changes of mycobiota community, and the resultant effects on the antioxidant properties during the Chinese bayberry Jiaosu fermentation. The structure and composition of mycobiota during the Chinese bayberry Jiaosu fermentation were significantly changed (p < 0.001) and clearly clustered into two distinct phases (Phase 1: Day 5–20; Phase 2: Day 30–60, p < 0.001). From Phase 1 to Phase 2, the dominant fungi gradually changed from Saccharomycetales fam Incertae sedis to Saccharomyces cerevisiae. The antioxidative properties (total polyphenols, 1,1-diphenyl-2-picrylhydrazyl [DPPH], superoxide and 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulphonate) [ABTS] radical scavenging) of Chinese bayberry Jiaosu were significantly increased by 250.4, 73.9, 25.3 and 40.0% respectively (p < 0.001). Co-occurrence network analysis revealed that Saccharomyces cerevisiae contributed to the increase of antioxidative properties in the Chinese bayberry Jiaosu fermentation. Our research indicates that fermentation into Chinese bayberry Jiaosu is an effective and new method for high-valued utilization of Chinese bayberry.

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Ultrasonic Extraction of Thai Propolis for Antimicrobial and Antioxidant Properties

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.506.371 ◽

2012 ◽

Vol 506 ◽

pp. 371-374 ◽

Cited By ~ 2

Author(s):

S. Sanpa ◽

Krit Sutjarittangtham ◽

T. Tunkasiri ◽

Sukum Eitssayeam ◽

P. Chantawannakul

Keyword(s):

Antioxidant Activities ◽

Biological Activities ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Extraction Methods ◽

Antimicrobial Activities ◽

Diffusion Method ◽

Inhibitory Effects ◽

Ultrasound Technology ◽

Efficient Extraction

Propolis is the mixture of resins that honeybees collect from plant buds, leaves and exudates. Antimicrobial and antioxidant properties of propolis have been widely studied, however, there have few reports on Thai propolis. To increase the efficient extraction of organic propolis compounds, extraction methods have been developed. We applied the ultrasound technology to reduce extraction time and increase extraction yields of propolis. The propolis was extracted using ultrasound technology for 15-60 minutes and the propolis extracts were examined to compare their biological activities. Antimicrobial activities of the propolis were investigated using the agar well diffusion method. The free radical-scavenging and antioxidant activities of propolis extracts were measured using the DPPH and FolinCiocalteau colorimetric methods. The propolis extracted using ultrasound for 15 and 30 minutes showed inhibitory effects on tested fungi and bacteria as well as antioxidant activities.

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Isolation, Characterization, and Biological Activity of some Fatty Acids and Volatile Oils from Iraqi Eucalyptus microtheca Plant

International Journal of Pharmaceutical Quality Assurance ◽

10.25258/ijpqa.11.1.21 ◽

2020 ◽

Vol 11 (01) ◽

pp. 136-140

Author(s):

Muthanna J. Mohammed ◽

Omar Ali Ali

Keyword(s):

Mass Spectrometry ◽

Gas Chromatography ◽

Vaccenic Acid ◽

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Gas Chromatography Mass Spectrometry ◽

Diffusion Method ◽

Disk Diffusion Method ◽

Eucalyptus Microtheca

This study aims at investigating the chemical composition, antimicrobial and antioxidant activity of some extracted essential and fixed oils from leaves of Eucalyptus microtheca plant grown in Iraq. Analysis of the isolated oils has been achieved by gas chromatography coupled with mass spectrometry gas chromatography–mass spectrometry (GC-MS) Technology. The study reveals existence of sixteen compounds. Camphene (20.60%), 4-carene (18.53%), 1,8-cineole (11.96%), terpin-4-ol (8.70%) and p-cymene (8.39%) were the highest components in these essential oils. While nine compounds were obtained as fixed oils, pentadecanoic acid (36.47%) and cis-vaccenic acid (30.31%) were the major components. The antimicrobial activity of the leaves extracts was evaluated against six different gram-positive and Gram-negative bacteria using disk diffusion method and exhibited good inhibition activity. Moreover, antioxidant assay (free radical scavenging activity) demonstrated good activity for the extracted oils. The results show that the aerial parts (leaves) of the Iraqi E. microtheca plant possess antibacterial and antioxidant properties and may suggest it as a good candidate to use for medicinal purposes.

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