scholarly journals Polyphenolic QTOF-ESI MS Characterization and the Antioxidant and Cytotoxic Activities of Prunus domestica Commercial Cultivars from Costa Rica

Molecules ◽  
2021 ◽  
Vol 26 (21) ◽  
pp. 6493
Author(s):  
Mirtha Navarro-Hoyos ◽  
Elizabeth Arnáez-Serrano ◽  
Silvia Quesada-Mora ◽  
Gabriela Azofeifa-Cordero ◽  
Krissia Wilhelm-Romero ◽  
...  
Keyword(s):  
Costa Rica ◽  
Cell Lines ◽  
Negative Correlation ◽  
Significant Negative Correlation ◽  
Total Phenolic ◽  
Prunus Domestica ◽  
Potential Health ◽  
Sw620 Cell ◽  
Significant Difference ◽  
Commercial Cultivars

There is an increased interest in plum research because of their metabolites’ potential bioactivities. In this study, the phenolic profiles of Prunus domestica commercial cultivars (Methley, Pisardii and Satsuma) in Costa Rica were determined by Ultra Performance Liquid Chromatography coupled with High Resolution Mass Spectrometry using a quadrupole-time-of-flight analyzer (UPLC-ESI-QTOF MS) on enriched phenolic extracts obtained through Pressurized Liquid Extraction (PLE) under acidic and neutral extraction conditions. In total, 41 different phenolic compounds were identified in the skin and flesh extracts, comprising 11 flavan-3-ols, 14 flavonoids and 16 hydroxycinnamic acids and derivatives. Neutral extractions for the skins and flesh from all of the cultivars yielded a larger number of compounds, and were particularly rich in the number of procyanidin trimers and tetramers when compared to the acid extractions. The total phenolic content (TPC) and antioxidant potential using the DPPH and ORAC methods exhibited better results for neutral extracts with Satsuma skins and Methley flesh, which showed the best values (685.0 and 801.6 mg GAE/g extract; IC50 = 4.85 and 4.39 µg/mL; and 12.55 and 12.22 mmol TE/g extract, respectively). A Two-Way ANOVA for cytotoxicity towards AGS gastric adenocarcinoma and SW620 colon adenocarcinoma indicated a significant difference (p < 0.05) for PLE conditions, with better results for neutral extractions, with Satsuma skin delivering the best results (IC50 = 60.7 and 46.7 µg/mL respectively) along with Methley flesh (IC50 = 76.3 and 60.9 µg/mL, respectively). In addition, a significant positive correlation was found between TPC and ORAC (r = 0.929, p < 0.05), as well as a significant negative correlation (p < 0.05) between TPC and cytotoxicity towards AGS and SW620 cell lines (r = −0.776, and −0.751, respectively). A particularly high, significant, negative correlation (p < 0.05) was found between the number of procyanidins and cytotoxicity against the AGS (r = −0.868) and SW620 (r = −0.855) cell lines. Finally, the PCA clearly corroborated that neutral extracts are a more homogenous group exhibiting higher antioxidant and cytotoxic results regardless of the part or cultivar; therefore, our findings suggest that PLE extracts under neutral conditions would be of interest for further studies on their potential health benefits.

scholarly journals HRMS Characterization, Antioxidant and Cytotoxic Activities of Polyphenols in Malus domestica Cultivars from Costa Rica

Molecules ◽  
2021 ◽  
Vol 26 (23) ◽  
pp. 7367
Author(s):  
Mirtha Navarro-Hoyos ◽  
Elizabeth Arnáez-Serrano ◽  
Silvia Quesada-Mora ◽  
Gabriela Azofeifa-Cordero ◽  
Krissia Wilhelm-Romero ◽  
...  
Keyword(s):  
Costa Rica ◽  
Cell Lines ◽  
Cancer Cell ◽  
Negative Correlation ◽  
Malus Domestica ◽  
Antioxidant Activities ◽  
Significant Negative Correlation ◽  
Cancer Cell Lines ◽  
Total Phenolic ◽  
Cytotoxic Activities

There is increasing interest in research into fruits as sources of secondary metabolites because of their potential bioactivities. In this study, the phenolic profiles of Malus domestica Anna and Jonagold cultivars from Costa Rica were determined by Ultra Performance Liquid Chromatography coupled with High Resolution Mass Spectrometry (HRMS) using a quadrupole-time-of-flight analyzer (UPLC-QTOF-ESI MS), on enriched-phenolic extracts from skins and flesh, obtained through Pressurized Liquid Extraction (PLE). In total, 48 different phenolic compounds were identified in the skin and flesh extracts, comprising 17 flavan-3-ols, 12 flavonoids, 4 chalcones, 1 glycosylated isoprenoid and 14 hydroxycinnamic acids and derivatives. Among extracts, the flesh of Jonagold exhibits a larger number of polyphenols and is especially rich in procyanidin trimers, tetramers and pentamers. Evaluating total phenolic content (TPC) and antioxidant activities using ORAC and DPPH procedures yields higher values for this extract (608.8 mg GAE/g extract; 14.80 mmol TE/g extract and IC50 = 3.96 µg/mL, respectively). In addition, cytotoxicity evaluated against SW620 colon cancer cell lines and AGS gastric cancer cell lines also delivered better effects for Jonagold flesh (IC50 = 62.4 and 60.0 µg/mL, respectively). In addition, a significant negative correlation (p < 0.05) was found between TPC and cytotoxicity values against SW620 and AGS adenocarcinoma (r = −0.908, and −0.902, respectively). Furthermore, a significant negative correlation (p < 0.05) was also found between the number of procyanidins and both antioxidant activities and cytotoxicity towards SW620 (r = −0.978) and AGS (r = −0.894) cell lines. These results align with Jonagold flesh exhibiting the highest abundance in procyanidin oligomers and yielding better cytotoxic and antioxidant results. In sum, our findings suggest the need for further studies on these Costa Rican apple extracts—and particularly on the extracts from Jonagold flesh—to increase the knowledge on their potential benefits for health.

scholarly journals Polyphenolic Characterization, Antioxidant, and Cytotoxic Activities of Mangifera indica Cultivars from Costa Rica

Foods ◽  
2019 ◽  
Vol 8 (9) ◽  
pp. 384 ◽  
Author(s):  
Mirtha Navarro ◽  
Elizabeth Arnaez ◽  
Ileana Moreira ◽  
Silvia Quesada ◽  
Gabriela Azofeifa ◽  
...  
Keyword(s):  
Costa Rica ◽  
Negative Correlation ◽  
High Resolution Mass Spectrometry ◽  
Mangifera Indica ◽  
Colon Adenocarcinoma ◽  
Significant Negative Correlation ◽  
Ultra Performance Liquid Chromatography ◽  
Total Phenolic ◽  
Cytotoxic Activities ◽  
Quercetin Derivatives

The phenolic profile of skin and flesh from Manifera indica main commercial cultivars (Keitt and Tommy Atkins) in Costa Rica was studied using ultra performance liquid chromatography coupled with high resolution mass spectrometry (UPLC-ESI-MS) on enriched phenolic extracts. A total of 71 different compounds were identified, including 32 gallates and gallotannins (of different polymerization degree, from galloyl hexose monomer up to decagalloyl hexoses and undecagalloyl hexoses); seven hydroxybenzophenone (maclurin and iriflophenone) derivatives, six xanthonoids (including isomangiferin and mangiferin derivatives); 11 phenolic acids (hydroxybenzoic and hydroxycinnamic acid derivatives); and eight flavonoids (rhamnetin and quercetin derivatives). The findings for T. Atkins skin constitute the first report of such a high number and diversity of compounds. Also, it is the first time that the presence of gallotannin decamers and undecamers are reported in the skin and flesh of Keitt cultivar and in T. Atkins skins. In addition, total phenolic content (TPC) was measured with high values especially for fruits’ skins, with a TPC of 698.65 and 644.17 mg gallic acid equivalents/g extract, respectively, for Keitt and T. Atkins cultivars. Antioxidant potential using 2,2-diphenyl-1-picrylhidrazyl (DPPH) and oxygen radical absorbance capacity (ORAC) methods were evaluated, with T. Atkins skin showing the best values for both DPPH (IC50 = 9.97 µg/mL) and ORAC (11.02 mmol TE/g extract). A significant negative correlation was found for samples between TPC and DPPH antioxidant values (r = −0.960, p < 0.05), as well as a significant positive correlation between TPC and ORAC (r = 0.910, p < 0.05) and between DPPH and ORAC antioxidant methods (r = 0.989, p < 0.05). Also, cytotoxicity was evaluated in gastric adenocarcinoma (AGS), hepatocarcinoma (HepG2), and colon adenocarcinoma (SW620), with T. Atkins skin showing the best results (IC50 = 138–175 µg/mL). Finally, for AGS and SW 620 cell lines particularly, a high significant negative correlation was found between cytotoxic activity and gallotannins (r = −0.977 and r = −0.940, respectively) while for the HepG2 cell line, the highest significant negative correlation was found with xanthonoids compounds (r = −0.921).

scholarly journals Characterization of Anthocyanins in Sweet Potato Leaves Grown in Various Stages and Conditions

2019 ◽  
pp. 253-262
Author(s):  
Xiaoyu Su ◽  
Zhenbao Jia ◽  
Fei Tao ◽  
Jiamin Shen ◽  
Jingwen Xu ◽  
...  
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Anthocyanin Biosynthesis ◽  
Total Phenolic ◽  
Growth Stages ◽  
Anthocyanin Content ◽  
Potential Health ◽  
Significant Difference ◽  
Young Leaves ◽  
Sweet Potato Leaves

Phytochemical-enriched edible greens, sweet potato leaves (Ipomoea batatas L.), have become popular due to potential health benefits. However, the phytochemical contents in sweet potato leaves and their subsequent change over harvest stages and growth condition are mostly unknown. In this study, the anthocyanin profile and content in leaves of four sweet potato cultivars, i.e., white-skinned and white-fleshed Bonita, red-skinned and orange-fleshed Beauregard, red-skinned and white-fleshed Murasaki and purple-skinned and purple-fleshed P40, were evaluated. Fourteen anthocyanins were isolated and identified by HPLC-MSI/MS. The most abundant was cyanidin 3-caffeoyl-p-hydroxybenzoyl sophoroside-5-glucoside, which comprised up to 20% of the total anthocyanins. Of the young leaves (1st and 2nd slip cuttings), Bonita contained the highest anthocyanin content followed by P40. Of the mature leaves (vine stage), Beauregard had the greatest anthocyanin (592.5 ± 86.4 mg/kg DW) and total phenolic (52.2 ± 3 mg GAE/g DW). It should be noted that the lowest anthocyanin and total phenolic content of shoots were found in P40, while tubers of P40 contain the highest content of each. Furthermore, the increase in leaf anthocyanin content over the growth stages that was observed in three of the cultivars but not in P40. No significant difference of anthocyanin content was found in Beauregard leaves grown in the high tunnels when compared with that in the open field. This study demonstrated for the first time that anthocyanin levels were significantly changed in response to various growth stages but not high tunnel condition, indicating that the effect of anthocyanin biosynthesis in sweet potato leaves is highly variable and genotype specific.

scholarly journals Yield and Safety of Transjugular Versus Percutaneous Liver Biopsies in Suspected Cases of Diffuse Liver Disease and Correlation of Yield of Transjugular Liver Biopsy with Hepatic Venous Pressure Gradient

2020 ◽  
Author(s):  
Mousam Dey ◽  
Simi Das ◽  
Argha Chatterjee ◽  
Agnibha Dutta ◽  
Ranajoy Ghosh ◽  
...  
Keyword(s):  
Liver Biopsy ◽  
Pressure Gradient ◽  
Negative Correlation ◽  
Diagnostic Yield ◽  
Venous Pressure ◽  
Major Complication ◽  
Significant Negative Correlation ◽  
Hepatic Venous Pressure Gradient ◽  
Transjugular Liver Biopsy ◽  
Significant Difference

Abstract Background Liver biopsy is indicated in both diagnosis and prognosis of diffuse liver diseases. Conventionally, percutaneous liver biopsy (PLB) is used, as it is easily available, affordable and has a shorter procedure time, whereas transjugular liver biopsy (TJLB) is used in the setting of ascites and coagulopathy. Our aim is to evaluate the diagnostic yield of TJLB in comparison to PLB with tract embolization. Our secondary aims were to evaluate whether there is any difference in rate of major and minor complications between the two procedures and evaluate whether there is any correlation between diagnostic yield of TJLB and hepatic venous pressure gradient (HVPG). Methods In this retrospective study, we included a total of consecutive 123 patients who underwent liver biopsy through percutaneous (n = 97) and transjugular route (n = 26). We compared the yield of the specimen based on the number of complete portal tracts (CPT). Results There was no significant difference between mean CPT in TJLB and PLB specimens (mean CPT of TJLB and PLB were 10.9 ± 2.7 and 11.6 ±2.5, respectively [p = 0.566]). There was a moderate but significant negative correlation between the total number of CPT and HVPG in the TJLB group (Spearman’s rho − 0.58) (p = 0.002). There was no statistically significant difference in minor complication between the two procedures. Only one patient who underwent PLB developed major complication and none of TLJB procedure had any major complication. Conclusion Yield of tissue and complication rates are comparable in TJLB and PLB groups. Yield of tissue in TJLB have intermediate but significant negative correlation with HVPG.

scholarly journals Calcitriol Elevation Is Associated with a Higher Risk of Refractory Hypercalcemia of Malignancy in Solid Tumors

2019 ◽  
Vol 105 (4) ◽  
pp. e1115-e1123 ◽  
Author(s):  
Tariq Chukir ◽  
Yi Liu ◽  
Katherine Hoffman ◽  
John P Bilezikian ◽  
Azeez Farooki
Keyword(s):  
Solid Tumors ◽  
Negative Correlation ◽  
Response Rate ◽  
Retrospective Chart Review ◽  
Significant Negative Correlation ◽  
Hematologic Malignancies ◽  
Antiresorptive Therapy ◽  
Hypercalcemia Of Malignancy ◽  
Metastatic Lesions ◽  
Significant Difference

Abstract Background Hypercalcemia of malignancy (HCM) is a common complication of advanced cancer. PTH-independent HCM may be mediated through different mechanisms: (1) humoral HCM, caused by the secretion of PTH-related peptide (PTHrP), (2) local osteolysis resulting from metastatic lesions, and (3) calcitriol-mediated hypercalcemia. Calcitriol-mediated HCM in patients with nonlymphomatous solid tumors is thought to be rare. Methods We performed a retrospective chart review from 2008 to 2017 to characterize further patients at our institution with solid tumors who had HCM with concomitant elevations in calcitriol. Patients with PTH-dependent hypercalcemia and patients with evidence of granulomatous disease were excluded, as were patients with hematologic malignancies. We hypothesized that patients with HCM and elevated calcitriol levels would respond less favorably to treatment with antiresorptive therapy compared with patients with HCM but without calcitriol elevation. We also aimed to assess mortality and determine if PTHrP and phosphorus levels correlate with calcitriol because both factors may alter calcitriol levels. Results Of 101 eligible patients, calcitriol was elevated in 45 (45%). PTHrP was elevated in 76% of patients with elevated calcitriol compared with 52% of patients without calcitriol elevation. The mean PTHrP value did not differ between patients with HCM and elevated calcitriol (36.3 ± 22 pg/mL) and those without calcitriol elevation (37.4 ± 19 pg/mL). Those with elevated calcitriol levels generally did not respond completely to antiresorptive treatment (80% incomplete response rate), whereas most patients without an elevation in calcitriol responded well to antiresorptive treatment (78% response rate: P &lt; .001). There was no significant difference in the percentage of patients with metastatic bone disease among the 2 groups (49% vs. 55%, respectively). There was no difference in mortality between the 2 groups (P = .14). A weak but significant negative correlation was found between phosphorus and calcitriol (Pearson r = -0.261, P = .016). This correlation was only significant in patients without calcitriol elevation (Pearson r = -0.4, P = .0082). Also, a significant negative correlation was found between PTHrP and phosphorus, again only in patients without calcitriol elevation. Discussion In the setting of HCM, patients with calcitriol elevation are much less likely to respond to antiresorptive therapy than patients without calcitriol elevation. Because calcitriol elevation did not appear to be correlated with hypophosphatemia or elevated PTHrP, it would appear that calcitriol production under these conditions is autonomous, and not subject to normal physiological controls. These observations indicate that calcitriol elevations in patients with HCM have clinical significance.

Eye-Head Coordination during Optokinetic Stimulation in Squirrel Monkeys

1981 ◽  
Vol 90 (1) ◽  
pp. 85-88 ◽  
Author(s):  
Takeshi Kubo ◽  
David W. Jensen ◽  
Makoto Igarashi ◽  
Jerry L. Homick
Keyword(s):  
Statistical Analysis ◽  
Eye Movements ◽  
Negative Correlation ◽  
Guinea Pigs ◽  
Significant Negative Correlation ◽  
Squirrel Monkeys ◽  
Slow Phase ◽  
Optokinetic Stimulation ◽  
The Gaze ◽  
Significant Difference

Head and eye movements in the yaw plane were recorded during and after optokinetic stimulation in squirrel monkeys. 1) Phasic or tonic head deviations to the side of the ocular quick phase occurred in 94% of total recordings (n = 50) during the perstimulus period, and in 75% of recordings (n = 49) during the poststimulus period. Magnitude of mean head deviation was significantly different between perstimulus and poststimulus periods. 2) Head nystagmus associated with eye nystagmus was consistently observed in seven of nine squirrel monkeys during optokinetic stimulation. Squirrel monkeys are thereby less prone to display head nystagmus than either guinea pigs, pigeons or chickens. 3) Slow phase speeds of coupled head and eye nystagmus were subjected to statistical analysis. A highly significant negative correlation was found between slow phase head and eye speeds. The correlation coefficient was −0.81 at 60°/sec stimulus (n = 119) and −0.72 at 100°/sec stimulus (n = 131). The gaze speed, calculated by summing the head and eye speeds, was 59.1 ± 6.8/sec at 60°/sec and 92.2 ± 11.4 at 100°/sec stimulus. There was no significant difference between the gaze speed in a free head condition and the eye speed when the head was fixed.

scholarly journals HEAVY METALS POLLUTION OF WHEAT FIELDS (SOIL AND LEAVES) SAMPLED FROM BASRAH AND MAYSAN PROVINCES

2020 ◽  
Vol 17 (35) ◽  
pp. 315-326
Author(s):  
Qusai Hattab MADHI ◽  
Mohammed Hamza ABASS ◽  
Abdulnabi Abdul Ameer MATROOD
Keyword(s):  
Heavy Metals ◽  
Soil Ph ◽  
Negative Correlation ◽  
Agricultural Soils ◽  
Significant Negative Correlation ◽  
Soil Characteristics ◽  
Lead Content ◽  
Significant Difference ◽  
Heavy Metals Pollution ◽  
Wheat Leaves

This study was conducted to estimate the level of some heavy metals, mainly Lead (Pb), cadmium (Cd), chromium (Cr) and cobalt (Co) in the soil and wheat leaves of some wheat fields in Basra and Maysan province; the sampling areas were Al-Qurna, Al-Madinah, Al-Amara, Kumit, Ali Al-Sharqi, and Ali Al-Gharbi. It was performed the analysis using the Flame Atomic Spectrophotometer. The results indicated an increase in the concentrations of above mentioned these heavy metals in all examined areas; the levels in agricultural soils were found to be exceeding the internationally permissible limits according to EU-2000 standards, the highest levels of pollution were observed at Al- Qurna site with significant differences than other sites for both available and total HMs concentrations in soils. Indicating that the arrangement of the metals according to their available concentrations in the analyzed soil was as follows: lead (21.32 )˃ cobalt (14.63) ˃ chromium (11.06) ˃ cadmium (1.15) as mg/Kg of soil. Additionally, results showed that the highest lead content in the wheat leaves was examined in the Qurna fields (0.175 mg/kg), followed by Amara with a concentration of 0.136 mg/kg. The lowest concentration of lead was observed in wheat leaves in the Kumit fields (0.007 mg/kg). In terms of Cd concentration in wheat leaves, the highest level was observed in Al-Qurna, with a significant difference from other fields, reaching 0.009 mg/kg. The lowest concentration of this HM was recorded in Ali Al-Gharbi, which reached 0.002 mg/kg. The results of the correlation between the available concentration of heavy metals elements and soil characteristics revealed a significant correlation between the soil pH and lead, cadmium, and chromium available concentrations, whereas no correlation with cobalt and a significant correlation between soil EC and lead was observed and a highly significant negative correlation with the cobalt. Results proved the high levels of pollution in all examined areas in Basra and Maysan provinces.

scholarly journals A Sense of Harassment and its Relationship to the Level of Professional Educational Adjustment among a Sample of Employees in the Directorate of Education in Damascus

2017 ◽  
Vol 11 (2) ◽  
pp. 278
Author(s):  
Raghdaa A. Naiseh
Keyword(s):  
Negative Correlation ◽  
Analytical Approach ◽  
Significant Negative Correlation ◽  
Female Workers ◽  
Significant Difference ◽  
Government Education ◽  
Institutional Sector ◽  
Males And Females ◽  
Level Of Significance ◽  
The Relationship

The study aimed to identify the level of a sense of harassment among a sample of workers and institutional sector of government education. It defines the professional level of compatibility among a sample of workers in farming Damascus Directorate. And the study of the relationship between the sense of harassment and professional compatibility among a sample of workers in farming Damascus Directorate. And to identify the level of significance of differences in the answers to the study sample the sense of harassment and scale professional compatibility scale according to the variables of the study: (sex, and marital status), and has been relying on the descriptive and analytical approach, and used two tools of the study: a sense of harassment measure, and the measure of professional compatibility, and amounted to a sample study (144) factor and running in working in the farming Damascus Directorate. Among the most important findings of the study were: The level of the sense of harassment among workers in the Directorate of Education in Damascus was moderate; the professional level of compatibility among workers in the Directorate of Education in Damascus was moderate; a statistically significant negative correlation between the sense of harassment and the professional compatibility; the presence of statistically significant difference between males and females on the sense of harassment and in favor of male workers; a statistically significant difference between the average of married and non-married on harassment and in favor of non-married employees; a statistically significant difference between the average of males and females on the compatibility and in favor of female workers. There was no statistically significant difference between the average married and non-married on the compatibility scal

Calling on Compensation in Australian Call Centres

2003 ◽  
Vol 9 (3) ◽  
pp. 62-76 ◽  
Author(s):  
Gary Crone ◽  
Lorraine Carey ◽  
Peter Dowling
Keyword(s):  
Customer Service ◽  
Negative Correlation ◽  
Employee Performance ◽  
Empirical Studies ◽  
Significant Negative Correlation ◽  
Telephone Call ◽  
Call Centre ◽  
Full Time ◽  
Call Centres ◽  
Significant Difference

ABSTRACTWhile there is a growing body of research on telephone call centre management in the U.K. and the U.S.A., empirical studies in Australia are at an embryonic stage. To date, most of the studies have focussed on the management of employee performance. The principal aim of this study was to provide data on current compensation practices in Australian call centres and to determine the extent of their strategic and best-practice orientation. A second aim was to explore whether the strategic management of compensation can help to balance the tension between commitment to customer service and commitment to employee motivation.Using data collected through a mail questionnaire survey of telephone call centres operating in a range of industries in Australia, the paper explores the effect of compensation practices on employee performance, absenteeism and turnover. Following a review of the literature on call centre management and the literature on compensation strategies, the findings are presented. Key findings include: a) a significant negative correlation between annual salary and the number of calls handled by full-time customer service representatives (CSRs); b) a significant positive correlation between casual CSRs' pay rates and turnover; c) a significant negative correlation between full-time CSRs' pay and absenteeism; d) a highly significant difference between the compensation strategies currently practiced in Australian call centres and the strategies call centre managers think should be practiced and e) Australian call centre managers report their compensation strategies are not very effective in increasing performance or employee satisfaction.

Interleukin 37 Levels in Relation to Serum Vitamin D Levels Among Ain Shams University Medical Students

QJM ◽  
2021 ◽  
Vol 114 (Supplement_1) ◽  
Author(s):  
Randa Reda Mabrouk ◽  
Afaf Abdelalim Mostafa ◽  
Dina Aly Mohamed Aly Ragab ◽  
Fouad Mohamed Fouad zaki
Keyword(s):  
Vitamin D ◽  
Medical Students ◽  
Vitamin D Deficiency ◽  
Negative Correlation ◽  
Serum Levels ◽  
Significant Negative Correlation ◽  
Group I ◽  
Vitamin D Levels ◽  
Significant Difference ◽  
Group Ii

Abstract Background The extraskeletal role of vitamin D is being increasingly recognized. This has important clinical implications, as vitamin D deficiency has reached epidemic proportions worldwide. Vitamin D has proposed anti-inflammatory properties as recent data suggests that low vitamin D concentrations are associated with increased levels of inflammatory markers. Interleukin-37(IL-37) is an IL1 family cytokine discovered in recent years and has 5 different isoforms. As an immunosuppressive factor, IL-37 can suppress excessive immune response .IL37 plays a role in protecting the body against endotoxin shock, ischemia reperfusion injury, autoimmune diseases, and cardiovascular diseases. In addition, IL-37 has a potential antitumor effect. IL-37 and its receptors may serve as novel targets for the study, diagnosis, and treatment of immune-related diseases and tumors. Aim of the Work The aim of this study is to determine the relation between the level of interleukin-37 and 25-hydroxy Vitamin D among Ain Shams University medical students. Subjects and Methods The study was conducted at Clinical Pathology Department, Ain Shams University Hospitals. Ninety individuals, from medical students of Ain Shams University who participated in the Nutritional Assessment of Ain Shams University Medical Students (NAMESASU) Project, were selected to be in the study. The study included 2 groups: Group I: included 45 subjects selected from the NAMES-ASU project with deficient vitamin D serum levels. Group II: included 45 subjects selected from the NAMES-ASU project with sufficient vitamin D serum levels. Results There was a highly significant difference between the two groups regarding vitamin D levels. Serum IL-37 levels were significantly higher in group I subjects compared to group II subjects. No significant difference was observed between group I and group II regarding BMI, BFM, PBF and hsCRP. No significant difference was observed between the two subgroups regarding IL-37 levels and hsCRP levels. A highly significant negative correlation was observed between vitamin D levels and IL-37. A significant negative correlation was observed between hsCRP and vitamin D levels. However, no correlation was observed between hsCRP and IL-37 levels. Conclusion Data from our study showed that present study denote that in case of vitamin D deficiency, irrespective of BMI, a subclinical state of inflammation may be present as reflected by the increased hsCRP levels and this state of inflammation might induce an increase in IL-37, an anti-inflammatory cytokine, in an attempt to reduce the inflammation.

Sign in / Sign up

Export Citation Format

Share Document