scholarly journals Hair EDX Analysis—A Promising Tool for Micronutrient Status Evaluation of Patients with IBD?

Nutrients ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 2572
Author(s):  
Georgiana-Emmanuela Gîlcă-Blanariu ◽  
Adina Coroabă ◽  
Manuela Ciocoiu ◽  
Anca Trifan ◽  
Gabriel Dimofte ◽  
...  
Keyword(s):  
Trace Elements ◽  
Disease Activity ◽  
Clinical Signs ◽  
Control Group ◽  
Healthy Controls ◽  
Micronutrient Deficiencies ◽  
Promising Tool ◽  
Micronutrient Status ◽  
Bowel Diseases ◽  
The Status

Micronutrient deficiencies can arise in various conditions, including inflammatory bowel diseases (IBD), and diagnosing these deficiencies can be challenging in the absence of specific clinical signs. The aim of this study was to evaluate the status of various trace elements hair concentration in IBD patients compared to a healthy control group and to identify potential correlations between the micronutrient status and relevant parameters related to disease activity. The concentrations of iron, magnesium, calcium, zinc, copper, manganese, selenium and sulfur in the hair of 37 IBD patients with prior diagnosed IBD (12 Crohn’s disease and 25 ulcerative colitis) and 31 healthy controls were evaluated by Energy Dispersive X-Ray spectroscopy (EDX). Significant differences in hair concentration profile of studied trace elements were identified for IBD patients compared to healthy controls. A significantly decreased hair concentration of iron, magnesium, calcium and selenium and a significantly increased sulfur hair concentration were observed in IBD patients at the time of evaluation. A decreased hair calcium concentration (r = −0.772, p = 0.003) and an increased sulfur concentration (r = 0.585, p = 0.046) were significantly correlated with disease activity. Conclusion: Hair mineral and trace elements evaluation may contribute to a proper evaluation of their status in IBD patients and improving the management of nutritional status of IBD patients.

scholarly journals Trace elements and rat pouchitis.

2012 ◽  
Vol 59 (4) ◽  
Author(s):  
Sławomira Drzymała-Czyż ◽  
Tomasz Banasiewicz ◽  
Stanisław Walas ◽  
Tomasz Kościński ◽  
Ewa Wenska-Chyży ◽  
...  
Keyword(s):  
Trace Elements ◽  
Restorative Proctocolectomy ◽  
Inductively Coupled Plasma ◽  
Complete Removal ◽  
Control Group ◽  
Slight Reduction ◽  
Inductively Coupled ◽  
The Status ◽  
Plasma Mass Spectrometry ◽  
Inflammatory Changes

The procedure of restorative proctocolectomy is associated with a complete removal of the colon and slight reduction of ileum length, which together can lead to systemic shortages of trace elements. Inflammatory changes in the pouch mucosa may also have some impact. However, there is no data on trace elements in pouchitis. Therefore, in the present study we aimed to assess the effect of acute pouchitis on the status of selected trace elements in rats. Restorative proctocolectomy with the construction of intestinal J-pouch was performed in twenty-four Wistar rats. Three weeks after the surgery, pouchitis was induced. Eight untreated rats created the control group. Liver concentrations of selected micronutrients (Zn, Cu, Co, Mn, Se) were measured in both groups six weeks later, using inductively coupled plasma mass spectrometry. Liver concentrations of trace elements did not differ between the study and the control groups. However, copper, cobalt and selenium concentrations [μg/g] were statistically lower (p

scholarly journals Evaluation of selected trace elements in the Moroccan type 2 diabetic patients

2019 ◽  
Vol 8 (1) ◽  
pp. 78
Author(s):  
Naoual El Omri ◽  
Fadwa Mekouar ◽  
Naoufal Assoufi ◽  
Abdelkhalek Maaroufi ◽  
Jihane Smaali ◽  
...  
Keyword(s):  
Trace Elements ◽  
Hdl Cholesterol ◽  
Control Group ◽  
Diabetic Patients ◽  
Type 2 Diabetic Patients ◽  
Type 2 Diabetics ◽  
The Status ◽  
Normal Metabolism ◽  
Serum Trace Elements

Background: Several trace elements have been implicated in the etiology of type 2 diabetes, they can influence the onset or pathogenesis of diabetes in various ways including disruption of normal metabolism of glucose and insulin. The purpose of this work is to evaluate the serum status of copper, zinc, selenium, chromium and manganese in Moroccan type 2 diabetics compared to control group.Methods: Blood samples from 100 type 2 diabetics and 90 control subjects were analyzed for blood glucose, glycated hemoglobin (only for diabetics), lipid status and serum trace elements concentrations.Results: Glucose and triglyceride values were statistically higher in diabetics; while those of HDL-cholesterol were lower. Concentrations of zinc, selenium, chromium, and manganese were lower in diabetics compared to controls. In contrast, copper concentrations were higher.Conclusions: The status of trace elements is impaired in type 2 diabetics compared to a control group.

Trace Element Status (Zinc, Copper, Selenium, Iron, Manganese) in Patients with Long-Term Home Parenteral Nutrition

2016 ◽  
Vol 69 (2) ◽  
pp. 120-124 ◽  
Author(s):  
Milan Dastych Jr. ◽  
Michal Šenkyřík ◽  
Milan Dastych ◽  
František Novák ◽  
Petr Wohl ◽  
...  
Keyword(s):  
Trace Elements ◽  
Parenteral Nutrition ◽  
Blood Plasma ◽  
Whole Blood ◽  
Statistical Significance ◽  
Home Parenteral Nutrition ◽  
Control Group ◽  
The Status ◽  
In Blood Plasma

Background: The objective of the present study was to determine concentrations of zinc (Zn), copper (Cu), iron (Fe), selenium (Se) in blood plasma and manganese (Mn) in the whole blood in patients with long-term home parenteral nutrition (HPN) in comparison to the control group. Patients and Methods: We examined 68 patients (16 men and 52 women) aged from 28 to 68 years on a long-term HPN lasting from 4 to 96 months. The short bowel syndrome was an indication for HPN. The daily doses of Zn, Cu, Fe, Se and Mn in the last 3 months were determined. Results: No significant differences in blood plasma were found for Zn, Cu and Fe in patients with HPN and in the control group (p > 0.05). The concentration of Mn in whole blood was significantly increased in HPN patients (p < 0.0001), while Se concentration in these patients was significantly decreased (p < 0.005). The concentration of Mn in the whole blood of 16 patients with cholestasis was significantly increased compared to the patients without cholestasis (p < 0.001). The Cu concentration was increased with no statistical significance. Conclusion: In long-term HPN, the status of trace elements in the patients has to be continually monitored and the daily substitution doses of these elements have to be flexibly adjusted. Dosing schedule needs to be adjusted especially in cases of cholestatic hepatopathy. A discussion about the optimal daily dose of Mn in patients on HPN is appropriate. For clinical practice, the availability of a substitution mixture of trace elements lacking Mn would be advantageous.

scholarly journals Homocysteine, trace elements and oxidant/antioxidant status in mild cognitively impaired elderly persons: a cross-sectional study

2015 ◽  
Vol 53 (4) ◽  
pp. 336-342 ◽  
Author(s):  
Hajar Negahdar ◽  
Seyed Reza Hosseini ◽  
Hadi Parsian ◽  
Farzan Kheirkhah ◽  
Abbas Mosapour ◽  
...  
Keyword(s):  
Trace Elements ◽  
Cognitive Impairment ◽  
Antioxidant Status ◽  
Clinical Signs ◽  
Cross Sectional Study ◽  
Control Group ◽  
Elderly Persons ◽  
Effective Parameters ◽  
Cross Sectional ◽  
Significant Difference

Abstract Introduction. Mild cognitive impairment (MCI) is the prodromal stage of Alzheimer’s disease (AD), so identification of the related risk factors can be helpful. There are controversial data regarding the serum oxidant/antioxidant status, trace elements and homocysteine (Hcy) as effective parameters in this disease, therefore the status of these factors was determined in this study. Methods. One hundred-twenty elderly persons with cognitive impairment and 120 elderly healthy persons who were differentiated using Mini-mental state examination (MMSE) participated in this study. The patients were divided into mild and moderate-to-severe cognitive impairment group. Serum antioxidant/oxidant, copper (Cu), manganese (Mn), zinc (Zn) and Hcy concentrations were measured using routine methods. Results. Oxidant and antioxidant levels increased and decreased based on the severity of the disease and were higher and lower in patients than in control group, respectively (p<0.001). With adjusting for age, gender and education, significant difference in Hcy levels was not observed. There was no significant difference in trace elements levels among groups. Conclusions. Results confirmed the association between oxidative damage with increasing the severity of cognitive impairment. These factors may be involved in the etiology of cognitive impairment and AD. Identification of such biomarkers is important to select appropriate treatment goals before the onset of irreversible clinical signs.

scholarly journals Cytokine Profile in Juvenile Systemic Lupus Erythematosus

2021 ◽  
pp. 72-78
Author(s):  
A. Elfar ◽  
Amal El-Bendary ◽  
M. A. Abdel-Hafez ◽  
N. Abo El Hana
Keyword(s):  
Lupus Nephritis ◽  
Disease Activity ◽  
Urinary Protein ◽  
Cytokine Profile ◽  
Control Group ◽  
Healthy Children ◽  
Healthy Controls ◽  
Sledai Score ◽  
Significant Difference ◽  
Active Patients

Background: Cytokines have an important role in immune system dysregulation in SLE because they act on the differentiation, maturation, and activation of several effector cells, culminating in inflammation and subsequent tissue damage.The aim of the work was to evaluate cytokine profile (IL2, IL10 and IL13) in children with SLE and their possible role in the pathogenesis of lupus nephritis. Methods: This is a cross sectional case-control study conducted on 60 children with SLE and 30 healthy children of matched age and sex served as a control group. The presence of lupus nephritis was confirmed by renal biopsy and histopathological examination. The SLE Disease Activity Index (SLEDAI) score for each patient was used to evaluate disease activity. Serum IL2, IL-10 & IL-13levels were measured using ELISA. Results: There was a significant increase in serum IL-10 levels in SLE patients compared to healthy controls and in patients with lupus nephritis compared to patients without lupus nephritis. Also, there were significant positive correlation between IL-10 and SLEDAI Score and between IL-10 and 24-hour urinary protein collection. There was no statistically significant difference in IL-2 levels in SLE patients compared to healthy controls. However, IL2 levels were significantly lower in active patients without lupus nephritis compared to active patients with lupus nephritis. There was no correlation between IL-2 and 24-hour urinary protein collection. The levels of IL13 were significantly higher in SLE patients compared to healthy controls and in patients with lupus nephritis compared to patients without lupus nephritis. There were significant positive correlations between Il 13 and SLEDAI Score and between IL-13and 24-hour urinary protein collection. Conclusions: Soluble IL10 and IL-13 could be used as a measure of disease activity. Further studies are needed to evaluate SLE pathophysiology including measurement of cytokine profile.

Correction of bleeding in the early stages of gestation depending on the status of the embryo and extraembryonic structures

2017 ◽  
pp. 22-24
Author(s):  
E.V. Kravchenko ◽  
Keyword(s):  
Pregnant Women ◽  
Ultrasonic Inspection ◽  
Clinical Signs ◽  
First Trimester ◽  
Fibrin Clot ◽  
Main Group ◽  
Control Group ◽  
Early Stages ◽  
The Status ◽  
Hemostatic Therapy

The objective: the study of the status of the embryo and extraembryonic structures for bleeding that is not associated with the detachment of the chorion in the first trimester of gestation. Patients and methods. Were examined 34 patients in the gestational age 6-9 weeks (main group) with bleeding from the genital tract, in which, at the primary ultrasonic inspection revealed no detachment of the chorion. The control group included 30 women with uncomplicated course And trimester of gestation. The average age of the pregnant women of the main group was 25.3±3.1 years in the control group 26.4±4.3 years. All women underwent standard clinical and laboratory examination. Results. Studies have shown that prognostic markers of unfavorable development of the embryo in the early stages of pregnancy bleeding not related to chorion detachment was not detected. For the purpose of hemostasis in pregnant of the main group of prescribed drugs traneksamic acid 0.25 mg 3-4 times a day for 3 days. Traneksam – antifbrinolytic tool is able to block not only plasminogen, and plasmin that has already formed. It also stimulates the synthesis of collagen, through which increases the elasticity of the fibrin clot. Pregnant women in both groups received vitamin E 1 capsule per day magnesium products, Magnicum 1 tablet 3 times a day, folic acid 400 micrograms per day. Assessing clinical signs of disease in dynamics of treatment, it should be noted that the hemostasis in the main group was achieved in an average of 2.3±0.7 days. Conclusion. Bleeding in early gestation, not connected with the detachment of the chorion, the use of Traneksam provides fast absolute hemostasis, which in turn contributes to the normalization of tone of the uterus and the physiological course of placentation. Key words: bleeding in early pregnancy, the chorion detachment, hemostasis, hemostatic therapy, Traneksam.

scholarly journals Immunoglobulin subtype-coated bacteria are correlated with the disease activity of inflammatory bowel disease

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yutaro Masu ◽  
Yoshitake Kanazawa ◽  
Yoichi Kakuta ◽  
Yusuke Shimoyama ◽  
Motoyuki Onodera ◽  
...  
Keyword(s):  
Ulcerative Colitis ◽  
Flow Cytometry ◽  
Disease Activity ◽  
Ribosomal Rna ◽  
Active Phase ◽  
Healthy Controls ◽  
Novel Treatment ◽  
Bowel Diseases ◽  
Inflammatory Bowel ◽  
Bacteroides Ovatus

AbstractImmune response involving various immunoglobulin (Ig) isotypes and subtypes to microbiome is involved in the pathogenesis and disease activity of inflammatory bowel diseases (IBDs). To clarify the presence of Ig-coated bacteria in the intestine and its association with disease activity in ulcerative colitis (UC) and Crohn’s disease (CD), we extracted and classified Ig-coated bacteria from fecal samples of 42 patients with IBD and 12 healthy controls (HCs) using flow cytometry and 16S ribosomal RNA sequence analysis. The percentage of bacteria coated with IgA and IgM was higher in patients with IBD than in HCs, and IgG-coated bacteria were found only in patients with IBD. Moreover, the percentages of bacteria coated with IgG1, IgG2, IgG3, and IgM in UC samples and IgG3, IgG4, and IgM in CD samples were correlated with disease activities. The proportions of Bacteroides ovatus and Streptococcus increased during the active phase of CD. Hence, the detailed analysis of Ig-coated bacteria and Ig subtypes using flow cytometry could aid in developing useful indicators of disease activity and identifying more disease-related bacteria, which could become novel treatment targets for IBDs.

scholarly journals Altered lipid peroxidation and antioxidative plasma activity in patients with severe diffuse toxic goiter

2003 ◽  
Vol 49 (5) ◽  
pp. 42-45
Author(s):  
T. I. Rodionova ◽  
M. A. Kostenko
Keyword(s):  
Lipid Peroxidation ◽  
Clinical Signs ◽  
Control Group ◽  
Healthy Individuals ◽  
The Status ◽  
Group 3 ◽  
Toxic Goiter ◽  
Altered Lipid ◽  
Group 1 ◽  
Plasma Activity

Lipid peroxidation (LPO) and antioxidative plasma activity (APA) were examined in 96 female patients with severe diffuse toxic goiter (DTG, Graves "disease) whose age was 20 to 40years and in a control group of healthy individuals, matched by age. The patients with DTG were found to have enhanced LPO and APA (p < 0.05). There was a correlation between the degree of impaired APA and the incidence of some symptoms of DTG, primarily the signs of cardiovascular lesion. Clear relationships were found between the duration of DTG and the status of LPO and APA. All the patients were divided into 3 groups. In addition to the conventional therapy, trimethasidine, 60 mg/day, was given to Group 1 patients (n = 32) for a month. Group 2patients (n = 34) took a-tocopherol for a month. Group 3 patients (n - 30) received the routine thyrostatic therapy with mercaptoimidazole drugs. The use of drugs with antioxidative mechanism of action was found to result in the normalization of the levels of LPO products and APA in patients with DTG and to ensure earlier disappearance of the clinical signs of cardiovascular lesion than in the controls. Trimethasidine showed an earlier effect (on the average, by 3-4 weeks of therapy) than did a-tocopherol.

Low Urine Secretion of Semaphorin3A In Lupus Patients With Proteinuria

2021 ◽  
Author(s):  
Doron Rimar ◽  
Merav Lidar ◽  
Nasrin Eiza ◽  
Adi D Sabag ◽  
Elias Toubi ◽  
...  
Keyword(s):  
Immune Responses ◽  
Disease Activity ◽  
Lupus Erythematosus ◽  
Renal Involvement ◽  
Control Group ◽  
Healthy Controls ◽  
Adaptive Immune ◽  
Urine Concentrations ◽  
Urine Levels

Abstract Background: Immune semaphorins are important players in controlling both innate and adaptive immune responses. The regulatory role of semaphorin3A (sema3A) in systemic lupus erythematosus (SLE), rheumatoid arthritis (RA) and other autoimmune diseases is widely reported. Decreased levels of serum sema3A were shown to be associated with SLE disease activity. Objectives: To assess urine concentrations of sema3A in SLE patients and its correlation with renal involvement and disease activity. Methods: Urine levels of sema3A were analyzed in 38 SLE patients of whom 13 had renal involvement and were compared to 10 healthy controls and 8 RA patients (disease control group). Results: The secretion of urine sema3A was found to be significantly lower in SLE patients compared to healthy controls and RA patients (4.9±3.9 ng/ml, 8.5±2.7 ng/ml, 9.85±1.7 ng/ml, respectively, p = 0.0006). Urine sema3A was significantly lower in SLE patients with lupus nephritis than in patients without nephritis (4.0±3.4 ng/ml vs 6.5±3.8 ng/ml, p=0.03). Urine sema3A was inversely correlated with proteinuria and SLE disease activity. Conclusion: Urine sema3A is decreased in lupus patients and should be further evaluated as a possible biomarker for disease activity and renal involvement.

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