Oxytetracycline versus Doxycycline Collagen Sponges Designed as Potential Carrier Supports in Biomedical Applications

Many research studies are directed toward developing safe and efficient collagen-based biomaterials as carriers for drug delivery systems. This article presents a comparative study of the properties of new collagen sponges prepared and characterized by different methods intended for biomedical applications. The structural integrity is one of the main properties for a biomaterial in order for it to be easily removed from the treated area. Thus, the effect of combining a natural polymer such as collagen with an antimicrobial drug such as oxytetracycline or doxycycline and glutaraldehyde as the chemical cross-linking agent influences the cross-linking degree of the material, which is in direct relation to its resistance to collagenase digestion, the drug kinetic release profile, and in vitro biocompatibility. The enzymatic degradation results identified oxytetracycline as the best inhibitor of collagenase when the collagen sponge was cross-linked with 0.5% glutaraldehyde. The drug release kinetics revealed an extended release of the antibiotic for oxytetracycline-loaded collagen sponges compared with doxycycline-loaded collagen sponges. Considering the behavior of differently prepared sponges, the collagen sponge with oxytetracycline and 0.5% glutaraldehyde could represent a viable polymeric support for the prevention/treatment of infections at the application site, favoring tissue regeneration.

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Synthesis and Evaluation of AlgNa-g-poly(QCL-co-HEMA) Hydrogels as Platform for Chondrocyte Proliferation and Controlled Release of Betamethasone

International Journal of Molecular Sciences ◽

10.3390/ijms22115730 ◽

2021 ◽

Vol 22 (11) ◽

pp. 5730

Author(s):

Jomarien García-Couce ◽

Marioly Vernhes ◽

Nancy Bada ◽

Lissette Agüero ◽

Oscar Valdés ◽

...

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Controlled Release ◽

Biomedical Applications ◽

Release Kinetics ◽

Cell Types ◽

Tissue Engineering Scaffolds ◽

Almost All ◽

Scanning Electron

Hydrogels obtained from combining different polymers are an interesting strategy for developing controlled release system platforms and tissue engineering scaffolds. In this study, the applicability of sodium alginate-g-(QCL-co-HEMA) hydrogels for these biomedical applications was evaluated. Hydrogels were synthesized by free-radical polymerization using a different concentration of the components. The hydrogels were characterized by Fourier transform-infrared spectroscopy, scanning electron microscopy, and a swelling degree. Betamethasone release as well as the in vitro cytocompatibility with chondrocytes and fibroblast cells were also evaluated. Scanning electron microscopy confirmed the porous surface morphology of the hydrogels in all cases. The swelling percent was determined at a different pH and was observed to be pH-sensitive. The controlled release behavior of betamethasone from the matrices was investigated in PBS media (pH = 7.4) and the drug was released in a controlled manner for up to 8 h. Human chondrocytes and fibroblasts were cultured on the hydrogels. The MTS assay showed that almost all hydrogels are cytocompatibles and an increase of proliferation in both cell types after one week of incubation was observed by the Live/Dead® assay. These results demonstrate that these hydrogels are attractive materials for pharmaceutical and biomedical applications due to their characteristics, their release kinetics, and biocompatibility.

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Surface Properties and Biocompatibility of Anodized Titanium with a Potential Pretreatment for Biomedical Applications

Metals ◽

10.3390/met11071090 ◽

2021 ◽

Vol 11 (7) ◽

pp. 1090

Author(s):

Bai-Hung Huang ◽

Yi-Jung Lu ◽

Wen-Chien Lan ◽

Muhammad Ruslin ◽

Hung-Yang Lin ◽

...

Keyword(s):

Porous Structure ◽

Biomedical Applications ◽

Grazing Incidence ◽

Acid Pretreatment ◽

Osteoblast Cells ◽

In Vitro Biocompatibility ◽

Porous Ti ◽

Diphenyltetrazolium Bromide ◽

Anodized Titanium

The effects of anodized titanium (Ti) with a potential hydrogen fluoride (HF) acid pretreatment through cathodization on the formation of nano-porous Ti dioxide (TiO2) layer were characterized using field-emission scanning electron microscopy, grazing incidence X-ray diffractometer, and contact angle goniometer. The biocompatibility was determined by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) test. Analytical results found that a well-aligned nano-porous structure was formed on the anodized Ti surface with HF pretreatment concentration above 0.5%. Microstructure of the nano-porous Ti dioxide surface generated by anodization with HF pretreatment was composed of anatase and rutile phases, while the anodized Ti sample with HF pretreatment concentration of 0.5% presented excellent hydrophilicity surface. An in-vitro biocompatibility also indicated that osteoblast cells grown on the surface of the anodized Ti sample with HF pretreatment increased with the increase of culture time. The filopodia of osteoblast cells not only adhered flat, but also tightly grabbed the nano-porous structure for promoting cell adhesion and proliferation. Therefore, the anodized Ti with HF pretreatment can form a functionalized surface with great biocompatibility for biomedical applications, particularly for dental implants.

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Preparation, Characterization and In Vitro Release Kinetics of Vancomycin Carried β-TCP/Chitosan Composite Microspheres Used as Bone Tissue Engineering Scaffolds

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.512-515.1821 ◽

2012 ◽

Vol 512-515 ◽

pp. 1821-1825

Author(s):

Lin Zhang ◽

Xue Min Cui ◽

Qing Feng Zan ◽

Li Min Dong ◽

Chen Wang ◽

...

Keyword(s):

Tissue Engineering ◽

Release Kinetics ◽

In Vitro Release ◽

Cross Linking ◽

Tissue Engineering Scaffolds ◽

Composite Microspheres ◽

Chitosan Composite ◽

Vitro Release

A novel microsphere scaffolds composed of chitosan and β-TCP containing vancomycin was designed and prepared. The β-TCP/chitosan composite microspheres were prepared by solid-in-water-in-oil (s/w/o) emulsion cross-linking method with or without pre-cross-linking process. The mode of vancomycin maintaining in the β-TCP/chitosan composite microspheres was detected by Fourier transform infrared spectroscopy (FTIR). The in vitro release curve of vancomycin in simulated body fluid (SBF) was estimated. The results revealed that the pre-cross-linking prepared microspheres possessed higher loading efficiency (LE) and encapsulation efficiency (EE) especially decreasing the previous burst mass of vancomycin in incipient release. These composite microspheres got excellent sphere and well surface roughness in morphology. Vancomycin was encapsulated in composite microspheres through absorption and cross-linking. While in-vitro release curves illustrated that vancomycin release depond on diffusing firstly and then on the degradation ratio later. The microspheres loading with vancomycin would be to restore bone defect, meanwhile to inhibit bacterium proliferation. These bioactive, degradable composite microspheres have potential applications in 3D tissue engineering of bone and other tissues in vitro and in vivo.

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3D Printed PLA/PCL/TiO2 Composite for Bone Replacement and Grafting

MRS Advances ◽

10.1557/adv.2018.375 ◽

2018 ◽

Vol 3 (40) ◽

pp. 2373-2378 ◽

Cited By ~ 9

Author(s):

Sandra E. Nájera ◽

Monica Michel ◽

Nam-Soo Kim

Keyword(s):

Biomedical Applications ◽

Fused Deposition Modeling ◽

Fracture Strain ◽

Fused Deposition ◽

In Vitro Biocompatibility ◽

Bone Replacement ◽

Deposition Modeling ◽

3D Printed ◽

The Stability

ABSTRACTPolymer composites of Polylactic acid (PLA) and poly-ε-caprolactone (PCL), containing small amounts of titanium oxide (TiO2) were developed for biomedical applications. These composite materials were prepared, and then printed using Fused Deposition Modeling (FDM). 3D printed structures were characterized to determine their mechanical properties and biocompatibility. DSC analysis yielded useful information regarding the immiscibility of the different polymers, and it was observed that the particles of TiO2 improved the stability of the polymers. The ultimate tensile strength and the fracture strain increased by adding TiO2 as a filler, resulting in values of approximately 45 MPa and 5.5 % elongation. The printed composites show excellent in vitro biocompatibility including cell proliferation and adhesion, and are therefore promising candidates to be used in the biomedical field for bone replacement procedures, due to their properties similar to those of cancellous bone.

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Enhanced Biocompatibility of GPC by Ion Implantation and Deposition

MRS Proceedings ◽

10.1557/proc-0908-oo16-04 ◽

2005 ◽

Vol 908 ◽

Author(s):

Robert Lee Zimmerman ◽

Ismet Gürhan ◽

Claudiu I. Muntele ◽

Daryush Ila ◽

Feyzan Özdal-Kurt ◽

...

Keyword(s):

Ion Implantation ◽

Biomedical Applications ◽

Heart Valves ◽

Cell Attachment ◽

Blood Stream ◽

Silver Deposition ◽

In Vitro Biocompatibility ◽

Artificial Heart Valves ◽

Model Cell

AbstractBiocompatible Glassy Polymeric Carbon (GPC) is used for artificial heart valves and in other biomedical applications. Although it is ideally suited for implants in the blood stream, tissue that normally forms around the moving parts of a GPC heart valve sometimes loses adhesion and creates embolisms downstream. Here we compare silver ion implantation and silver deposition, each of which strongly inhibits cell attachment on GPC. Inhibition of cell adhesion is a desirable improvement to current GPC cardiac implants. In vitro biocompatibility tests have been carried out with model cell lines to demonstrate that traces of silver can favorably influence the surface of GPC for biomedical applications.

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Erratum to: Photocurable O-carboxymethyl chitosan derivatives for biomedical applications: Synthesis, in vitro biocompatibility, and their wound healing effects

Macromolecular Research ◽

10.1007/s13233-012-0204-1 ◽

2012 ◽

Vol 20 (11) ◽

pp. 1209-1209 ◽

Cited By ~ 1

Author(s):

Ha-Na Na ◽

Shin-Hye Park ◽

Kwang-Il Kim ◽

Mi Kyung Kim ◽

Tae-Il Son

Keyword(s):

Wound Healing ◽

Biomedical Applications ◽

Carboxymethyl Chitosan ◽

Chitosan Derivatives ◽

In Vitro Biocompatibility

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DEVELOPMENT AND CHARACTERIZATION OF GASTRO RETENTIVE MUCOADHESIVE MICROBEADS CONTAINING SIMVASTATIN WITH DIFFERENT CROSS LINKING AGENTS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2020v12i6.38913 ◽

2020 ◽

pp. 118-126

Author(s):

VENKATA RAMANA REDDY K. ◽

NAGABHUSHANAM M. V. ◽

PAMULA REDDY B. ◽

RAVINDAR NAIK E.

Keyword(s):

Drug Release ◽

Release Kinetics ◽

Drug Loading ◽

Aluminium Chloride ◽

In Vitro Dissolution ◽

Cross Linking ◽

Stability Studies ◽

Study Results ◽

Curing Agents

Objective: The aim of the present work was to prepare and examine drug release of the oral controlled release microbeads using different curing agents by emulsification internal ionic gelation technique. Methods: Cross-linked alginate microbeads were prepared with different cross linking agents by using mucoadhesive properties. The formation and compatibility of microbeads were confirmed by compatibility studies. Prepared microbeads evaluated for encapsulated efficiency, micromeritic properties, drug loading, in vitro wash off studies, in vitro dissolution studies, drug release kinetics and stability studies Results: The in vitro drug release was influenced by both type of curing agents and type of polymers and no significant changes in characterization parameters was observed after 3 mo stability studies. The sustained release profile of optimized batch was found to be 99.66±0.18% in comparison to pure drug profile of 28.64±0.02% at 12 h release study. Results of both wash-off and in vitro studies suggests that batch (SF2) prepared with aluminium chloride has shown better mucoadhesive property. Drug release of optimized batch follows zero order with non fickian mechanism according to Korsmeyer-Peppas equation. Conclusion: The data suggest the use of simvastatin mucoadhesive cross linked microbeads to offer the potential for oral controlled drug delivery with improved gastric retention and capable to provide sustained drug release by using cross linking agents.

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Chitosan inserts for periodontitis: Influence of drug loading, plasticizer and crosslinking on in vitro metronidazole release

Acta Pharmaceutica ◽

10.2478/v10007-007-0037-1 ◽

2007 ◽

Vol 57 (4) ◽

pp. 469-477 ◽

Cited By ~ 16

Author(s):

Romi Barat ◽

Anegundha Srinatha ◽

Jayanta Pandit ◽

Shampa Anupurba ◽

Neelam Mittal

Keyword(s):

Drug Release ◽

Release Kinetics ◽

Drug Loading ◽

Dissolution Time ◽

Cross Linking ◽

Burst Release ◽

Casting Method ◽

The Mean ◽

Polymer Ratio

Chitosan inserts for periodontitis: Influence of drug loading, plasticizer and crosslinking onin vitrometronidazole releaseChitosan based metronidazole (MZ) inserts were fabricated by the casting method and characterized with respect to mass and thickness uniformity, metronidazole loading andin vitrometronidazole release kinetics. The fabricated inserts exhibited satisfactory physical characteristics. The mass of inserts was in the range of 5.63 ± 0.42 to 6.04 ± 0.89 mg. The thickness ranged from 0.46 ± 0.06 to 0.49 ± 0.08 mm. Metronidazole loading was in the range of 0.98 ± 0.09 to 1.07 ± 0.07 mg except for batch CM3 with MZ loading of 2.01 ± 0.08 mg. The inserts exhibited an initial burst release at the end of 24 h, irrespective of the drug to polymer ratio, plasticizer content or cross-linking. However, further drug release was sustained over the next 6 days. Cross-linking with 10% (m/m) of glutaraldehyde inhibited the burst release by ~30% and increased the mean dissolution time (MDT) from 0.67 to 8.59 days. The decrease in drug release was a result of reduced permeability of chitosan due to cross-linking.

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