scholarly journals Mechanistic Insight of Allantoin in Protecting Tomato Plants Against Ultraviolet C Stress

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 11
Author(s):  
Mona F. A. Dawood ◽  
Md. Tahjib-Ul-Arif ◽  
Abdullah Al Mamun Sohag ◽  
Arafat Abdel Hamed Abdel Latef ◽  
Marwa M. Ragaey
Keyword(s):  
Plant Growth ◽  
Oxidative Damage ◽  
Principal Component ◽  
Soluble Carbohydrate ◽  
Enzymatic Antioxidants ◽  
Stress Markers ◽  
Solanum Lycopersicum L ◽  
Ultraviolet C ◽  
Purine Metabolite ◽  
Pre Treatment

Allantoin ((AT) a purine metabolite)-mediated ultraviolet C (UVC) stress mitigation has not been studied to date. Here, we reported the physicochemical mechanisms of UVC-induced stress in tomato (Solanum lycopersicum L.) plants, including an AT-directed mitigation strategy. UVC stress reduced plant growth and photosynthetic pigments. Heatmap and principal component analysis (PCA) revealed that these toxic impacts were triggered by the greater oxidative damage and disruption of osmolyte homeostasis. However, pre-treatment of AT noticeably ameliorated the stress-induced toxicity as evident by enhanced chlorophyll, soluble protein, and soluble carbohydrate contents in AT-pretreated UVC-stressed plants relative to only stressed plants leading to the improvement of the plant growth and biomass. Moreover, AT pre-treatment enhanced endogenous AT and allantoate content, phenylalanine ammonia-lyase, non-enzymatic antioxidants, and the enzymatic antioxidants leading to reduced oxidative stress markers compared with only stressed plants, indicating the protective effect of AT against oxidative damage. Moreover, PCA displayed that the protective roles of AT strongly associate with the improved antioxidants. On the other hand, post-treatment of AT showed less efficacy in UVC stress mitigation relative to pre-treatment of AT. Overall, this finding illustrated that AT pre-treatment could be an effective way to counteract the UVC stress in tomato, and perhaps in other crop plants.

scholarly journals Silicon Alleviates Copper Toxicity in Flax Plants by Up-Regulating Antioxidant Defense and Secondary Metabolites and Decreasing Oxidative Damage

2020 ◽  
Vol 12 (11) ◽  
pp. 4732 ◽  
Author(s):  
Hossam S. El-Beltagi ◽  
Mahmoud R. Sofy ◽  
Mohammed I. Aldaej ◽  
Heba I. Mohamed
Keyword(s):  
Lipid Peroxidation ◽  
Plant Growth ◽  
Oxidative Damage ◽  
Antioxidant Defense ◽  
Growth Yield ◽  
Enzymatic Antioxidants ◽  
Oxygen Species ◽  
Total Chlorophyll ◽  
Yield Attributes ◽  
Cu Toxicity

In recent years, nutrient management has gained much attention as a way to mitigate heavy metal stress. Silicon (Si) promotes plant defense responses against toxic metal stresses. In this study, we evaluated the effects of silicon (Si) on copper (Cu) toxicity in two flax genotypes (Sakha 1 and Sakha 2) as it relates to plant growth, yield attributes, total chlorophyll, nucleic acid content, enzymatic and non-enzymatic antioxidants, oxidative damage, lipid peroxidation, copper and silicon content, and fatty acid composition. The results showed that Cu (100 and 200 µM) inhibited plant growth and increased Cu accumulation in soil, roots, and shoots. Cu significantly decreased the yield attributes, total chlorophyll by 9.5% and 22% in Sakha 1 and by 22.5% and 29% in Sakha 2, and enhanced the accumulation of non-enzymatic (tocopherol), enzymatic antioxidants such as superoxide dismnutase, peroxidase, ascorbate peroxidase and catalase) and secondary metabolites (phenol and flavonoids). The DNA content significantly decreased in stressed plants with 100 and 200 µM Cu about 22% and 44%, respectively, in Sakha 1 and about 21.6% and 34.7% in Sakha 2, and RNA content also decreased by about 20% and 29%, respectively, in Sakha 1 and by about 2% and 13% in Sakha 2 compared to the control plant. Furthermore, Cu stress accelerated the generation of reactive oxygen species (ROS), such as hydrogen peroxide (H2O2) and induced cellular oxidative injury caused by lipid peroxidation. In parallel, Cu induced a change in the composition of fatty acids, resulting in lower unsaturated fatty acid levels and increased saturated fatty acids (increased saturation/unsaturation ratio for both genotypes). Treating the flax plants with irrigation three times with Si protected the plants from Cu toxicity. Si treatment decreased the uptake and the transport of Cu to the shoots and harvested seeds and promoted plant growth, yield attributes, and antioxidant defense systems by reducing Cu accumulation, lipid peroxidation, and the generation of H2O2. In addition, the alleviation of Cu toxicity correlated with increased Si accumulation in the roots and shoots. In conclusion, Si can be used to improve the resistance of flax plants to Cu toxicity by up-regulating the antioxidant defense system such as superoxide dismutase (SOD), peroxidase (POD), ascorbate peroxidase (APX) and catalase (CAT) and decreasing the oxidative damage caused by reactive oxygen species (ROS).

scholarly journals Assessment of Morpho-Physiological, Biochemical and Antioxidant Responses of Tomato Landraces to Salinity Stress

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 696
Author(s):  
Reem H. Alzahib ◽  
Hussein M. Migdadi ◽  
Abdullah A. Al Ghamdi ◽  
Mona S. Alwahibi ◽  
Abdullah A. Ibrahim ◽  
...  
Keyword(s):  
Salt Stress ◽  
Salinity Stress ◽  
Dry Weight ◽  
Proline Content ◽  
Enzymatic Antioxidants ◽  
Sod Activity ◽  
Carotene Content ◽  
Antioxidants Activity ◽  
Solanum Lycopersicum L ◽  
Salinity Levels

Understanding salt tolerance in tomato (Solanum lycopersicum L.) landraces will facilitate their use in genetic improvement. The study assessed the morpho-physiological variability of Hail tomato landraces in response to different salinity levels at seedling stages and recommended a tomato salt-tolerant landrace for future breeding programs. Three tomato landraces, Hail 548, Hail 747, and Hail 1072 were tested under three salinity levels: 75, 150, and 300 mM NaCl. Salinity stress reduced shoots’ fresh and dry weight by 71% and 72%, and roots were 86.5% and 78.6%, respectively. There was 22% reduced chlorophyll content, carotene content by 18.6%, and anthocyanin by 41.1%. Proline content increased for stressed treatments. The 300 mM NaCl treatment recorded the most proline content increases (67.37 mg/g fresh weight), with a percent increase in proline reaching 61.67% in Hail 747. Superoxide dismutase (SOD) activity decreased by 65% in Hail 548, while it relatively increased in Hail 747 and Hail 1072 treated with 300 mM NaCl. Catalase (CAT) activity was enhanced by salt stress in Hail 548 and recorded 7.6%, increasing at 75 and 5.1% at 300 mM NaCl. It revealed a reduction in malondialdehyde (MDA) at the 300 mM NaCl concentration in both Hail 548 and Hail 1072 landraces. Increasing salt concentrations showed a reduction in transpiration rate of 70.55%, 7.13% in stomatal conductance, and 72.34% in photosynthetic rate. K+/Na+ ratios decreased from 56% for 75 mM NaCl to 85% for 300 mM NaCl treatments in all genotypes. The response to salt stress in landraces involved some modifications in morphology, physiology, and metabolism. The landrace Hail 548 may have better protection against salt stress and observed protection against reactive oxygen species (ROS) by increasing enzymatic “antioxidants” activity under salt stress.

scholarly journals Exogenous Putrescine Enhances Salt Tolerance and Ginsenosides Content in Korean Ginseng (Panax ginseng Meyer) Sprouts

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1313
Author(s):  
Md. Jahirul Islam ◽  
Byeong Ryeol Ryu ◽  
Md. Obyedul Kalam Azad ◽  
Md. Hafizur Rahman ◽  
Md. Soyel Rana ◽  
...  
Keyword(s):  
Panax Ginseng ◽  
Salinity Stress ◽  
Soluble Sugar ◽  
Principal Component ◽  
Total Soluble Protein ◽  
Soluble Carbohydrate ◽  
Guaiacol Peroxidase ◽  
Salinity Treatment ◽  
Total Soluble Sugar ◽  
Biochemical Processes

The effect of exogenously applied putrescine (Put) on salt stress tolerance was investigated in Panax ginseng. Thirty-day-old ginseng sprouts were grown in salinized nutrient solution (150 mM NaCl) for five days, while the control sprouts were grown in nutrients solution. Putrescine (0.3, 0.6, and 0.9 mM) was sprayed on the plants once at the onset of salinity treatment, whereas control plants were sprayed with water only. Ginseng seedlings tested under salinity exhibited reduced plant growth and biomass production, which was directly interlinked with reduced chlorophyll and chlorophyll fluorescence due to higher reactive oxygen species (hydrogen peroxide; H2O2) and lipid peroxidation (malondialdehyde; MDA) production. Application of Put enhanced accumulation of proline, total soluble carbohydrate, total soluble sugar and total soluble protein. At the same time, activities of antioxidant enzymes like superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase in leaves, stems, and roots of ginseng seedlings were increased. Such modulation of physio-biochemical processes reduced the level of H2O2 and MDA, which indicates a successful adaptation of ginseng seedlings to salinity stress. Moreover, protopanaxadiol (PPD) ginsenosides enhanced by both salinity stress and exogenous Put treatment. On the other hand, protopanaxatriol (PPT) ginsenosides enhanced in roots and reduced in leaves and stems under salinity stress condition. In contrast, they enhanced by exogenous Put application in all parts of the plants for most cases, also evidenced by principal component analysis. Collectively, our findings provide an important prospect for the use of Put in modulating salinity tolerance and ginsenosides content in ginseng sprouts.

Relationship between atherogenic index and oxidative stress among patients presented with coronary artery disease in a tertiary care hospital

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 2807-2813
Author(s):  
Resmi C R ◽  
Kedari G S R ◽  
Deepa P K
Keyword(s):  
Oxidative Stress ◽  
Vitamin E ◽  
Vitamin C ◽  
Density Lipoprotein ◽  
Atherogenic Index ◽  
Enzymatic Antioxidants ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Lipid Parameters ◽  
And Oxidative Stress

CAD is recognized as a multifactorial disease that is influenced by environmental and genetic factors. This study aimed to evaluate the levels of lipid parameters, oxidative stress and antioxidant markers in subjects with CAD compared to their age & sex matched controls and to analyze the relationship between atherogenic Index and oxidative stress among them 62 clinically proved CAD patients and 62 healthy age and sex matched subjects without CAD were selected for this study. 5 ml of fasting venous blood was collected from all the subjects and investigations such as FPG, lipid profile, oxidative markers Malondialdehyde (MDA), F2 isoprostanes (F2iso) and antioxidants glutathione S-transferase (GST), superoxide dismutase (SOD), vitamin-C, vitamin-E were performed. This study showed that levels of lipid parameters total cholesterol (TC), triglycerides (TG), low density lipoprotein cholesterol (LDL-c) and AI were significantly higher whereas high density lipoprotein cholesterol (HDL-c) were significantly low in CAD patients compared to normal controls. Oxidative stress markers MDA and F2 Isoprostanes level were significantly high, whereas enzymatic antioxidants GST and SOD and non-enzymatic antioxidants Vitamin-C and Vitamin-E levels were significantly low in CAD patients. Oxidative stress markers were found to significantly influence the AI. Results of this study showed that oxidative stress markers F2iso and MDA and antioxidants GST, VIT-C and VIT-E are found to influence the atherogenic index significantly.

scholarly journals Role of Ferrous Sulfate (FeSO4) in Resistance to Cadmium Stress in Two Rice (Oryza sativa L.) Genotypes

Biomolecules ◽  
2020 ◽  
Vol 10 (12) ◽  
pp. 1693
Author(s):  
Javaria Afzal ◽  
Muhammad Hamzah Saleem ◽  
Fatima Batool ◽  
Ali Mohamed Elyamine ◽  
Muhammad Shoaib Rana ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gas Exchange ◽  
Plant Growth ◽  
Photosynthetic Pigments ◽  
Ferrous Sulfate ◽  
Oryza Sativa L ◽  
Enzymatic Antioxidants ◽  
Cd Stress ◽  
Ultra Structure ◽  
Gas Exchange Characteristics

The impact of heavy metal, i.e., cadmium (Cd), on the growth, photosynthetic pigments, gas exchange characteristics, oxidative stress biomarkers, and antioxidants machinery (enzymatic and non-enzymatic antioxidants), ions uptake, organic acids exudation, and ultra-structure of membranous bounded organelles of two rice (Oryza sativa L.) genotypes (Shan 63 and Lu 9803) were investigated with and without the exogenous application of ferrous sulfate (FeSO4). Two O. sativa genotypes were grown under different levels of CdCl2 [0 (no Cd), 50 and 100 µM] and then treated with exogenously supplemented ferrous sulfate (FeSO4) [0 (no Fe), 50 and 100 µM] for 21 days. The results revealed that Cd stress significantly (p < 0.05) affected plant growth and biomass, photosynthetic pigments, gas exchange characteristics, affected antioxidant machinery, sugar contents, and ions uptake/accumulation, and destroy the ultra-structure of many membranous bounded organelles. The findings also showed that Cd toxicity induces oxidative stress biomarkers, i.e., malondialdehyde (MDA) contents, hydrogen peroxide (H2O2) initiation, and electrolyte leakage (%), which was also manifested by increasing the enzymatic antioxidants, i.e., superoxidase dismutase (SOD), peroxidase (POD), catalase (CAT) and ascorbate peroxidase (APX) and non-enzymatic antioxidant compounds (phenolics, flavonoids, ascorbic acid, and anthocyanin) and organic acids exudation pattern in both O. sativa genotypes. At the same time, the results also elucidated that the O. sativa genotypes Lu 9803 are more tolerant to Cd stress than Shan 63. Although, results also illustrated that the exogenous application of ferrous sulfate (FeSO4) also decreased Cd toxicity in both O. sativa genotypes by increasing antioxidant capacity and thus improved the plant growth and biomass, photosynthetic pigments, gas exchange characteristics, and decrease oxidative stress in the roots and shoots of O. sativa genotypes. Here, we conclude that the exogenous supplementation of FeSO4 under short-term exposure of Cd stress significantly improved plant growth and biomass, photosynthetic pigments, gas exchange characteristics, regulate antioxidant defense system, and essential nutrients uptake and maintained the ultra-structure of membranous bounded organelles in O. sativa genotypes.

scholarly journals Social Hierarchy Effects on Stress Responses of Subordinate Specimens in Sea Bream (Sparus aurata)

2021 ◽  
Author(s):  
Maria Dioguardi ◽  
Mirella Vazzana ◽  
Mariano Dara ◽  
Irene Vazzana ◽  
Davide Accardi ◽  
...  
Keyword(s):  
Stress Responses ◽  
Phagocytic Activity ◽  
Social Stress ◽  
Physiological Stress ◽  
Sparus Aurata ◽  
Principal Component ◽  
Social Hierarchy ◽  
Sea Bream ◽  
Stress Markers ◽  
Environmental Variations

Abstract: Social stress can affect the ability of the fish to respond to various stressors, such as pathogens or environmental variations. In this paper, the effects of social stress on gilt-head bream (Sparus aurata) were investigated. To study the effects of physiological stress, we evaluated biochemical and cellular parameters as cortisol, glucose, lactate, osmolarity and phagocytosis 24 hours after the establishment of social hierarchy. Social hierarchy was determined and characterised by behavioural observation (aggressive acts and feeding order) of the specimens (dominant &ldquo;&alpha;&rdquo;, subordinate &ldquo;&beta;&rdquo; and &ldquo;&gamma;&rdquo;). After the establishment of the social hierarchy, we observed that the levels of plasma cortisol and other biochemical stress markers (glucose and lactate) were higher in subordinate individuals than in dominant ones. In addition, the modulation of phagocytic activity of the peritoneal cavity cells (PEC) demonstrated that social stress appeared to affect the immune response. At last, principal component analysis clearly separated the subordinate fish groups from the dominant groups based on stress markers and phagocytic activity of the peritoneal exudates cells.

scholarly journals An assessment of atmospheric deposition of metals and the physico -chemical parameters of a rainwater harvesting system in Rio de Janeiro Brazil, by means of statistical multivariate analysis

2020 ◽  
Vol 15 (4) ◽  
pp. 1
Author(s):  
Gabrielle Nunes da Silva ◽  
Letícia Delduque Alves ◽  
Isabella Escobar dos Santos ◽  
Daniele Maia Bila ◽  
Alfredo Akira Ohnuma Júnior ◽  
...  
Keyword(s):  
Rio De Janeiro ◽  
Fine Particles ◽  
Rainwater Harvesting ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
High Concentration ◽  
Physico Chemical ◽  
Pearson Coefficient ◽  
Coefficient Corrélation ◽  
Pre Treatment

This study evaluates the rainwater harvesting system to determine the chemical quality of the water and allocate it for use. Samples were collected in the city of Rio de Janeiro at the points of Direct Precipitation (DP), First Flush (FF) and Reservoir (RR). The methodology involved carrying out an analysis of Ca, Cd, Cu, Na, Ni, Fe, K, Mg, Pb, Zn, conductivity, turbidity, pH and chloride. The R language was used for the principal component analysis, Pearson coefficient correlation, hierarchical cluster analysis, Tukey test and boxplot. The results show that when there is no interference from the catchment surface (DP), the rainwater has a low concentration of metals, although the first millimeters of rain have a concentration above what is permissible in the guidelines issued by the Ministry of Health (Ordinance n° 05/2017). The disposal system (FF) reduced the concentration of pollutants in the RR, but not enough to comply with legislation. It was noted that Ca, K and Na are the main metals found in rainwater. During the dry period, there were significant correlations between the physico-chemical and meteorological parameters. The high concentration of metals in the wet period suggests that rain assists the cleaning of the atmosphere and that most metals are present in the form of aerosols or fine particles suspended in the air. The findings reveal significant indications of acid rain and pre-treatment is recommended to ensure rainwater can be used safely for more mundane purposes.

scholarly journals Assessment of wild tomato accessions for fruit yield, physicochemical and nutritional properties under a rain forest agro-ecology

2020 ◽  
Vol 1 (2) ◽  
pp. 1-11
Author(s):  
Dorcas Ibitoye ◽  
Adesike Kolawole ◽  
Roseline Feyisola
Keyword(s):  
Rain Forest ◽  
Agronomic Traits ◽  
Block Design ◽  
Principal Component ◽  
Fruit Yield ◽  
Soluble Solids ◽  
Forest Zone ◽  
Breeding Lines ◽  
Wild Tomato ◽  
Solanum Lycopersicum L

Tomato (Solanum lycopersicum L.) is a broadly consumed fruit vegetable globally. It is one of the research mandate vegetable of the National Horticultural Research Institute (NIHORT), Ibadan, Nigeria. The institute’s contains diverse collections of tomato accessions and wild relatives, without utilization information for the African continent. With the decline in diversity and potential of cultivars, a robust tomato breeding pipeline with broad genetic base that eliminates redundancy in the development of lines with desired horticultural traits is paramount. This study evaluated the mean performance and variations of thirteen wild tomato accessions obtained from the C.M. Rick Tomato Genetic Resource Center, University of California, Davis, USA, evaluated for agronomic, nutritional and physicochemical traits under a rain forest zone in Nigeria. The accessions were planted and grown in three replications with randomized complete block design. Agronomic traits, physicochemical and nutritional parameters were measured and analyzed. There was significant (P < 0.001) variation among accessions for all traits measured. Accession LA0130 was separated from others by cluster analysis and was outstanding for its unique attributes which include: fruit yield parameters, total soluble solids, acidity and content. The principal component analysis suggests fruit yield related traits, acidity and contributed most to the variation among the 13 accessions. The results obtained can be used to breed materials adapted to a rain forest . These wild tomato accessions have genes with desirable agronomic, nutritional and physicochemical traits that could be into breeding lines to improve commercial tomato varieties.

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