scholarly journals Accumulation of Anthocyanins and Other Phytochemicals in American Elderberry Cultivars during Fruit Ripening and its Impact on Color Expression

Plants ◽  
2020 ◽  
Vol 9 (12) ◽  
pp. 1721
Author(s):  
Yucheng Zhou ◽  
Yu Gary Gao ◽  
M. Monica Giusti
Keyword(s):  
Phenolic Content ◽  
Differential Method ◽  
Total Phenolic ◽  
Coumaric Acid ◽  
Native Plant ◽  
Ripe Fruit ◽  
Fruit Extract ◽  
Natural Colorant ◽  
Plate Reader ◽  
Acylated Anthocyanins

American elderberry (Sambucus canadensis) is a plant native to North America with anthocyanin-rich fruits. Our objective was to investigate the effects of cultivar and ripeness on the phytochemical characteristics of its fruits and the corresponding color performance. Cultivars ‘Adams’, ‘Johns’, ‘Nova’, ‘Wyldewood’, and ‘York’ were examined for their °Brix, pH, anthocyanin (pH-differential method), and phenolic content (Folin-Ciocalteau method). Extract composition were analyzed by uHPLC-PDA-MS/MS. Color and spectra were determined using a plate reader. All characteristics evaluated were significantly affected by ripeness and cultivar, except for °Brix and total phenolic content, which did not vary significantly among cultivars. Most anthocyanins (63–72%) were acylated with p-coumaric acid, with cyanidin-3-(trans)-coumaroylsambubioside-5-glucoside the most predominant. The proportion of acylated anthocyanins was the only characteristic evaluated that decreased during ripening (from 80 to 70%). Extract from fully-ripened fruits exhibited red (lvis-max ~520 nm) and blue hues (lvis-max ~600 nm) at acidic and alkaline pH, respectively. Extracts from half-ripe fruit rendered yellowish tones and overall dull color. C-18 semi-purified extracts displayed higher color saturation (smaller L* and larger C*ab) than crude extracts. The vibrant and broad color expression of fully-ripened fruit extract, especially after C-18 purification, suggests this North American native plant as a promising natural colorant source.

scholarly journals New insights of phenolic compounds from optimized fruit extract of Ficus auriculata

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Shahinuzzaman ◽  
Parul Akhtar ◽  
N. Amin ◽  
Yunus Ahmed ◽  
Farah Hannan Anuar ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Bioactive Compounds ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Optimum Extraction

AbstractIn this study, the extraction conditions extracted maximize amounts of phenolic and bioactive compounds from the fruit extract of Ficus auriculata by using optimized response surface methodology. The antioxidant capacity was evaluated through the assay of radical scavenging ability on DPPH and ABTS as well as reducing power assays on total phenolic content (TPC). For the extraction purpose, the ultrasonic assisted extraction technique was employed. A second-order polynomial model satisfactorily fitted to the experimental findings concerning antioxidant activity (R2 = 0.968, P < 0.0001) and total phenolic content (R2 = 0.961, P < 0.0001), indicating a significant correlation between the experimental and expected value. The highest DPPH radical scavenging activity was achieved 85.20 ± 0.96% at the optimum extraction parameters of 52.5% ethanol (v/v), 40.0 °C temperature, and 22 min extraction time. Alternatively, the highest yield of total phenolic content was found 31.65 ± 0.94 mg GAE/g DF at the optimum extraction conditions. From the LC–ESI–MS profiling of the optimized extract, 18 bioactive compounds were tentatively identified, which may regulate the antioxidant activity of fruits of F. auriculata.

scholarly journals The Effect of High Pressure Processing on Polyphenol Oxidase Activity, Phytochemicals and Proximate Composition of Irish Potato Cultivars

Foods ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 517
Author(s):  
Konstantina Tsikrika ◽  
Nora O’Brien ◽  
Dilip K. Rai
Keyword(s):  
High Pressure ◽  
Polyphenol Oxidase ◽  
Proximate Composition ◽  
Phenolic Content ◽  
High Pressure Processing ◽  
Irish Potato ◽  
Potato Cultivars ◽  
Simultaneous Increase ◽  
Total Phenolic ◽  
Coumaric Acid

Polyphenol oxidase (PPO) activity, proximate composition, and phytochemicals were determined in four common Irish potato cultivars following a high pressure processing (HPP) at 600 MPa for 3 min. PPO activity was significantly (p < 0.05) lower in all HPP treated samples, while the overall proximate composition was not affected. The total phenolic content was significantly higher in the HPP treated samples. Chlorogenic acid levels significantly decreased with simultaneous increase of caffeic acid and p-coumaric acid levels upon HPP treatment. No significant changes were observed in rutin and ferulic acid levels, although their levels varied, depending on the potato cultivars, while the levels of cytotoxic glycoalkaloids (α-solanine and α-chaconine) remained unaltered.

scholarly journals Maclura pomifera (Osage Orange) Meyve Özütünden Elektroeğirme Yöntemiyle Üretilen Membranların Karakterizasyonu

2018 ◽  
Vol 6 (4) ◽  
pp. 447
Author(s):  
Rifat Battaloğlu ◽  
Emine Müge Pekacar
Keyword(s):  
Total Phenolic Content ◽  
Phenolic Content ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Binding Properties ◽  
Maclura Pomifera ◽  
Electrospinning Method ◽  
Phenolic Substances ◽  
Ft Ir ◽  
Resistant Structure

In this study, electrospinning method which is a common method of obtaining nanofibers is used. Nanolif was synthesized with chitosan-ethylenediamine tetraacetic acid / polyvinylalcohol (CS-EDTA / PVA) polymers using macula pomifera fruit extract. To obtain the nanofibers, 3% macula pomifera extract was added to the CS-EDTA / PVA solution. The structure and diameters of the resulting nanofibers were visualized using a scanning electron microscope (SEM). Chemical binding properties were determined by Fourier Transform Infrared Spectroscopy (FT-IR). Mass change due to nanofiber temperature was performed by Thermogravimetric analysis (TGA). At the end of the study, the total phenolic content of the extract and nanofiber was determined. The time dependent changes in the total phenolic content of nanolife have been investigated. The results obtained show that the nanofiber is loaded with the extract. It turns out that nanolipine is a temperature resistant structure. In addition, the phenolic compounds transfer to the nanofiber structure in a small amount of the extract. However, it has been determined that there is no significant change in the total amount of phenolic substances after nanofiber formation.

scholarly journals Chemical Standardization and Anti-Proliferative Activity of Ardisia elliptica Fruit against the HCT116 Human Colon Cancer Cell Line

Molecules ◽  
2020 ◽  
Vol 25 (5) ◽  
pp. 1023
Author(s):  
Suchanuch Ondee ◽  
Pongtip Sithisarn ◽  
Supachoke Mangmool ◽  
Piyanuch Rojsanga
Keyword(s):  
Proliferative Activity ◽  
Phenolic Content ◽  
Mrna Level ◽  
Total Phenolic ◽  
Caspase 8 ◽  
Fruit Extract ◽  
Human Colon Cancer ◽  
Hct 116 ◽  
Apoptotic Genes ◽  
Hct 116 Cells

The present study is intended to carry out the chemical standardization and evaluation of the anti-proliferative activity of A. elliptica fruit extract. A. elliptica fruit powder was extracted with ethanol. The obtained extract was assessed for total phenolic content using the Folin–Ciocalteu method. Moreover, a simple, accurate, and precise reversed phase high-performance liquid chromatographic method was developed and validated to determine the embelin content of A. elliptica fruit extract. Then, the extract and embelin were investigated for their anti-proliferative effect against HCT-116 cells. Finally, the mechanisms of inhibition of the extract and embelin on the mRNA expression of pro-apoptotic genes Bad, Bax, and Caspase-8 and anti-apoptotic genes c-IAP1, Mcl-1, and XIAP were determined by real-time qRT-PCR. The phenolic content and embelin content of the extract were 5.20 ± 0.01 g of gallic acid equivalent per 100 g of dried fruit (g% GAE) and 5.57 ± 0.56 mg/g of extract, respectively. The extract and embelin showed strong anti-proliferative effects on HCT-116 cells with 50% inhibition concentration (IC50) values of 19.16 ± 1.09 µg/mL and 25.93 ± 1.75 µg/mL, respectively. The A. elliptica extract exhibited a significant increase in the mRNA level of Bad, Bax, and Caspase-8 and a significant decrease in c-IAP1, Mcl-1, and XIAP. Embelin showed a significant decrease in Mcl-1 and XIAP.

scholarly journals Phytochemical Analysis and Antimicrobial Activity of Origanum dictamnus Traditional Herbal Tea (decoction)

2017 ◽  
Vol 12 (11) ◽  
pp. 1934578X1701201
Author(s):  
Myrto Varsani ◽  
Konstantia Graikou ◽  
Aristea Velegraki ◽  
Ioanna Chinou
Keyword(s):  
Antimicrobial Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Biological Properties ◽  
Total Phenolic ◽  
Phytochemical Analysis ◽  
Coumaric Acid ◽  
Herbal Tea ◽  
Three Samples ◽  
First Time

In the framework of the re-evaluation of chemistry and biological properties of Greek herbal teas (decoctions), the objective of this study was the estimation of the total phenolic content and the antimicrobial activity as well as the chemical analysis of the water extracts (decoction) of three samples of Cretan dittany ( Origanum dictamnus L) prepared in the traditional way to be used (drunk). Ten secondary metabolites were isolated and elucidated on the basis of spectral evidence for the first time from O. dictamnus drinkable decoction: carvacrol (1), β-sitosterol (2), uvaol (3), ursolic acid (4), rosmarinic acid (5), lithospermic acid (6), ferulic acid (7), cafeic acid (8), p-coumaric acid (9) and apigenin-7- O-glucoside (10). The samples showed a high total phenolic content (185.5-139.7 mg CAE/g) and an interesting antibacterial profile against Gram positive and negative human pathogenic microorganisms. Furthermore, the bioactivity against a panel of Malassezia strains was estimated.

Comparison of berry composition of selected Vaccinium species (Ericaceae) with Gaylussacia dumosa1This article is part of a Special Issue entitled “A tribute to Sam Vander Kloet FLS: Pure and applied research from blueberries to heathland ecology”.

Botany ◽  
2012 ◽  
Vol 90 (5) ◽  
pp. 355-363 ◽  
Author(s):  
Charles F. Forney ◽  
Wilhelmina Kalt ◽  
Sam P. Vander Kloet
Keyword(s):  
Volatile Compounds ◽  
Applied Research ◽  
Phenolic Content ◽  
Vaccinium Corymbosum ◽  
Total Phenolic ◽  
Ripe Fruit ◽  
Fruit Composition ◽  
Commercial Species ◽  
Berry Composition ◽  
The Tropics

The genus Vaccinium contains a wide array of plants distributed throughout the world including several commercial species as well as epiphytic shrubs and lianas in the tropics. Vaccinium dumosum Andrews was reclassified as Gaylussacia dumosa (Andrews) Torr. & A. Gray in 1843 owing to its single seeded 10-locular ovary. Since then, evidence suggests G. dumosa be returned to Vaccinium. In this study, fruit composition of G. dumosa was compared with that of Vaccinium corymbosum L., Vaccinium angustifolium Aiton, Vaccinium gaulteriifolium (Griff.) Hook. f., Vaccinium acrobracteatum K. Sch., and Vaccinium hirsutum Buckley. Ripe fruit from each species were collected and analyzed. In each of the species, primary sugars were glucose and fructose, and acids were quinic, malic, and citric. Total phenolic content correlated with antioxidant capacity. Anthocyanin profiles showed that fruit from each of the species studied contained no pelargonidin glucosides, which are common in fruit of other genera. A total of 27 volatile compounds, dominated by terpenes and several esters, were detected. These volatiles were all found in four of the species including G. dumosa. No volatile compounds were detected in G. dumosa that were not found in the Vaccinium species. Gaylussacia dumosa showed no chemical composition that was significantly different than that of the Vaccinium species surveyed, suggesting that G. dumosa is aligned with Vaccinium.

scholarly journals Comparative antioxidant studies of methanol pericarp, mesocarp, seed and whole-fruit extracts and fractions of Citrullus lanatus

2021 ◽  
Vol 7 (1) ◽  
pp. 36-40
Author(s):  
EO Ettebong ◽  
◽  
GB Inyang ◽  
PS Thomas ◽  
AIL Bassey ◽  
...  
Keyword(s):  
Ascorbic Acid ◽  
Ethyl Acetate ◽  
Phenolic Content ◽  
Radical Scavenging ◽  
Seed Extract ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Aqueous Fraction ◽  
Antioxidant Power ◽  
Fruit Extracts

Aim: This study evaluated the antioxidant potentials of methanol seed, pericarp, mesocarp, and whole-fruit extracts and fractions of Citrillus lanatus. Methods: Various extracts and fractions (dichloromethane, ethyl acetate, n-butanol and aqueous) of Citrillus lanatus whole-fruit were investigated for 1, 1-diphenyl-2-picrylhydrazyl (DPPH) scavenging activities, total phenolic content (TPC) and ferric reducing antioxidant power (FRAP). Results: In the DPPH assay, the seed extract showed the highest activity (48%) followed by the whole-fruit extract (27%), pericarp (26%) and the mesocarp (22%) at 100 ug/ml. The whole-fruit fractions also showed improved activity with DCM fraction (64%), ethyl acetate (62%), aqueous fraction (46%) and n-butanol (24%) at 100 ug/ml but the activity observed with ascorbic acid, was much higher (84%). The seed extract had the highest phenolic content (14.30 mg) gallic acid equivalents per gram followed by the pericarp (9.58 mg/g), whole-fruit (6.94 mg/g) and mesocarp (2.78 mg/g). The DCM fraction exhibited the highest TPC (78.19 mg/g) followed by ethyl acetate (61.11 mg/g), aqueous (26.66 mg/g) and n-butanol (10.69 mg/g). FRAP assay showed strongest activity with the whole-fruit extract (0.640nm) followed by seed, mesocarp and pericarp extracts. The DCM fraction showed the highest antioxidant potential (0.735nm) followed by ethyl acetate, aqueous and n-butanol fractions but not comparable to ascorbic acid. Conclusion: The results revealed that C. lanatus seeds, pericarp, mesocarp and whole-fruit extracts and fractions contain varying amounts of flavonoids, tannins and phenolic compounds which exhibit potent antioxidant and free radical scavenging activities.

scholarly journals Bee Bread: Physicochemical Characterization and Phenolic Content Extraction Optimization

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1358 ◽  
Author(s):  
Florina Dranca ◽  
Florin Ursachi ◽  
Mircea Oroian
Keyword(s):  
Fatty Acids ◽  
Phenolic Content ◽  
Physicochemical Characterization ◽  
Maximum Level ◽  
Dienoic Acid ◽  
Extraction Yield ◽  
Total Phenolic ◽  
Coumaric Acid ◽  
Content Extraction ◽  
Bee Bread

Beebread or ambrosia is a unique product for humans and bees, which is the result of lactic fermentation on pollen in honeycombs. Bee bread is a rich source of nutrients (proteins, vitamins) and polyphenols (such as flavonoids, flavonols, phenolic acids). This study aimed to characterize bee bread in terms of physicochemical properties: pH, free acidity, glucose, fructose, sucrose, raffinose and melesitose content, total phenolic content (TPC), total flavones content (TFC), fatty acids and individual phenolics (gallic acid, protocatechiuc acid, p-hydroxybenzoic acid, caffeic acid, vanillic acid, chlorogenic acid, p-coumaric acid, rosmarinic acid, myricetin, luteolin, quercetin and kaempferol). The main phenolic compound identified in the bee bread was kaempferol, followed by myricetin and luteolin. The TPC, TFC and extraction yield were optimized in function of ultrasonic amplitude, temperature and time and the suitable conditions for achieving the maximum level were 87.20% amplitude of ultrasonic treatment, 64.70 °C and 23.10 min, respectively for reaching 146.2 mg GAE/L of TPC, 1231.5 mg QE/g of TFC and a 5.72% extraction yield. The most abundant fatty acids were C18:3 (all-cis-9,12,15) octadeca-6,9,15-trienoic acid, followed by C16:1 (9Z)-hexadec-9-enoic acid, C21:0 heneicosanoic acid and C18:2 (all-cis-9,12) (9Z,12Z)-octadeca-9,12-dienoic acid, respectively.

scholarly journals Comparative Polyphenol Composition, Antioxidant and Anticorrosion Properties in Various Parts of Panax ginseng Extracted in Different Solvents

2020 ◽  
Vol 11 (1) ◽  
pp. 93
Author(s):  
Ramalingam Malathy ◽  
Mayakrishnan Prabakaran ◽  
Kathirvel Kalaiselvi ◽  
Ill-Min Chung ◽  
Seung-Hyun Kim
Keyword(s):  
Panax Ginseng ◽  
Corrosion Inhibition ◽  
Animal Health ◽  
Vital Role ◽  
Total Phenolic ◽  
Coumaric Acid ◽  
Fruit Extract ◽  
Corrosive Media ◽  
Phenolic Components ◽  
Ultrahigh Performance Liquid Chromatography

Panax ginseng C.A. (P. ginseng) Meyer has been in use since ancient times for its therapeutic activities. Although several studies have investigated the roles of phytoconstituents in human and animal health, no comparative studies have been conducted to test the efficacy of P.ginseng leaf, fruit and root. Therefore, this study aimed to identify the antioxidant and anticorrosion activities of ginseng prepared using five different solvents. The methanolic fruit extract showed comparatively good activity in all assays. The total phenolic content (TPC) was higher in fruit (95.21 mg/g), followed by leaf (39.21 mg/g) extracted in methanol solvent compared other solvents. The total flavonoid content (TFC) of fruit methanolic extract was 50.21 mg/g, which was followed by fruit extracted in ethanol (41.33 mg/g). The same phenomenon was observed in all antioxidant studies. Through Ultrahigh Performance Liquid Chromatography (UHPLC), the presence of 23 phenolic components categorized as hydroxycinnamic acids, hydroxybenzoic acids and a few other groups that play a vital role in antioxidation was identified. Phenols such as chlorogenic acid (1002.2 μg/g), gentisic acid (854.21 μg/g) and rutin (165.32 μg/g) were found in higher amounts in fruit whereas leaf showed significant amounts of m-coumaric acid (185.32 μg/g) and p-coumaric acid (125.24 μg/g). The anti-corrosive property of the fruit extract of the ginseng with methanol as a solvent was analyzed for a copper specimen exposed to 1 M HCl medium and found to have 96% corrosion inhibition efficiency at a 1000-ppm concentration. The smooth surface of the specimen exposed to corrosive media shown in a field emission scanning electron microscope (FESEM) image confirms that the specimen was protected from corrosion, and energy-dispersive X-ray spectroscopy (EDX) spectra show that the loss of Cu is reduced in inhibited metal surface. Atomic force microscopy (AFM) images and surface roughness factor also validate the corrosion inhibition characteristic of ginseng plant extract.

scholarly journals Determination of total phenolic content and antioxidant activity of fruit mix from Malino, Gowa Regency, South Sulawesi

2021 ◽  
Vol 911 (1) ◽  
pp. 012085
Author(s):  
Risma Eva Cahyanti ◽  
Yusminah Hala ◽  
A. Mu’nisa
Keyword(s):  
Antioxidant Activity ◽  
Total Phenolic Content ◽  
Phenolic Content ◽  
Passion Fruit ◽  
Total Phenolic ◽  
Fruit Extract ◽  
Fruit Extracts ◽  
Dpph Method ◽  
Purple Passion Fruit

Abstract This study aimed to determine the total phenolic content and antioxidant activity of single fruit extracts and mixtures of tomatoes (Solanum lycopersicum), purple passion fruit (Passiflora edulis var. Sims), and strawberries (Fragaria sp.). Tomatoes, purple passion fruit and strawberries were extracted using 96% ethanol as solvent using the maceration method. Determination of total phenolic content using the Folin-ciocalteu method, measurement of antioxidant activity using the DPPH method (1.1 diphenyl-2-picrylhydrazil) spectrophotometrically and measuring the degree of acidity (pH) using a pH meter. Data were analyzed using ANOVA with Tukey’s further test. The results showed that the total phenolic content of tomatoes, purple passion fruit and strawberries before and after mixing were tomato extract (1,731 mg GAE/g), purple passion fruit extract (1,577 mg GAE/g), strawberry extract (1,917 mg GAE)./g), tomato and purple passion fruit extract (1,758 mg GAE/g), tomato and strawberry extract (2,020 mg GAE/g), strawberry and purple passion fruit extract (1,924 mg GAE/g) and tomato, purple passion fruit extract and strawberries (2.107 mg GAE/g). The antioxidant activity showed that there was a significant difference between the purple passion fruit treatments (78.695%), tomatoes and strawberries (86.160%) and tomatoes, purple passion fruit and strawberries (88.328%), but not significantly different from the tomatoes (80.683 %), tomatoes and purple passion fruit (82,059 %) as well as the treatment of strawberries (83.690 %), strawberries and purple passion fruit (84.097 %), but significantly different from the BHA control (93.526 %). It can be concluded that the total phenolic content and antioxidant activity of the mixed extracts of the three fruits, namely tomatoes, purple passion fruit and strawberries, were higher than those of the single fruit extracts without mixing.

Sign in / Sign up

Export Citation Format

Share Document