scholarly journals Cyanidin-3-glucoside Lipophilic Conjugates for Topical Application: Tuning the Antimicrobial Activities with Fatty Acid Chain Length

Processes ◽  
2021 ◽  
Vol 9 (2) ◽  
pp. 340
Author(s):  
Hélder Oliveira ◽  
Patrícia Correia ◽  
Lucinda J. Bessa ◽  
Marta Guimarães ◽  
Paula Gameiro ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Fatty Acid ◽  
Antioxidant Capacity ◽  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Aliphatic Chain ◽  
Fatty Acid Chain ◽  
The Stability ◽  
Application Tuning ◽  
Chain Lengths

Background: Natural anthocyanins present a low solubility in lipophilic media, which compromises their effective application in lipophilic systems. In this work, cyanidin-3-O-glucoside (Cy3glc) was esterified by the addition of fatty acids with increasing chain-lengths and a structure-activity relationship was performed towards the description of the best analog for skin-care applications. Methods: By enzymatic hemi-synthesis, it was possible to obtain 5 structurally related derivatives of cyanidin-3-O-glucoside with successive C2 increments in the aliphatic chain. The stability in hanks buffer and DMEM with or without FBS was followed by HPLC. The cytotoxicity against keratinocytes was evaluated by MTT assay. The antioxidant capacity was determined by using the fluorescent probe DCF-DA. The effect on enzyme activity was evaluated towards tyrosinase, collagenase, and elastase enzymes by colorimetric assays. MIC and MBC values were obtained against reference strains and against multidrug-resistant isolates. Results: In physiological conditions, cy3glc−fatty acid derivatives are more stable and may be converted to the native anthocyanin. The 5 conjugates showed lower antioxidant capacity and enzymatic inhibitory activities in comparison to the anthocyanin precursor. However, concerning the antibacterial activity, the insertion of a fatty acid chain sprouted the antibacterial activity, showing a clear biphasic effect and a more effective effect on Gram-positive bacteria. Conclusions: Cy3glc-C10 was the most effective compound considering the antimicrobial activity, although a general reduction was observed among the other activities evaluated. This work prompt further assays with a different panoply of derivatives ranging other features including saturation vs. unsaturation, even vs. odd carbon content and linear vs. branched.

scholarly journals Antibacterial Activity and Physicochemical Properties of Florally Diversified Bangladeshi Honeys

2018 ◽  
Vol 33 (1-2) ◽  
pp. 5-9 ◽  
Author(s):  
Roushney Fatima Mukti ◽  
Md Miraj Kobad Chowdhury ◽  
Md Ausrafuggaman Nahid ◽  
Md Mizanur Rahaman ◽  
M Aftab Uddin
Keyword(s):  
Ascorbic Acid ◽  
Antibacterial Activity ◽  
Physicochemical Properties ◽  
Antioxidant Capacity ◽  
Total Antioxidant Capacity ◽  
Antibacterial Activities ◽  
Multidrug Resistant ◽  
Bacterial Strains ◽  
Total Antioxidant ◽  
Phenolics Content

Seven florally diversified Bangladeshi honey samples were assessed for their physicochemical properties as well as their antibiogram profile on different human pathogenic bacterial strains. The average density, total protein content, ascorbic acid content, total phenolics content and the total antioxidant capacity of these honeys were determined as 1.50±0.09 g/ml, 5.63±1.56 mg/g, 91.87±22.16 mg/g, 571.04±289.02 mg gallic acid equivalent/gram, and 320.74±55.06 mg ascorbic acid equivalent/gram of samples, respectively. A significant correlation between the phenolics content and the total antioxidant capacity was observed in commercially available honey samples. However, the natural honey samples showed the best antibacterial activity against different pathogenic multidrug-resistant bacterial strains. Significant antibacterial activities were observed against Klebsiella pneumoniae, Salmonella typhi, Micrococcus luteus, Pseudomonas spp. and Enterobacter spp. and moderate antibacterial activities was observed on Shigella boydii, Bacillus cereus, Bacillus megaterium, and Bacillus subtilis. The antibacterial activity was correlated with the total antioxidant capacity. This study suggested that the Bangladeshi multifloral honeys have clinical potential against multidrug-resistant pathogenic bacterial strains. Bangladesh J Microbiol, Volume 33, Number 1-2, June-Dec 2016, pp 5-9

scholarly journals Chitosan-Stabilized Ag Nanoparticles with Superior Biocompatibility and Their Synergistic Antibacterial Effect in Mixtures with Essential Oils

Nanomaterials ◽  
2018 ◽  
Vol 8 (10) ◽  
pp. 826 ◽  
Author(s):  
Ludmila Cinteza ◽  
Cristina Scomoroscenco ◽  
Sorina Voicu ◽  
Cristina Nistor ◽  
Sabina Nitu ◽  
...  
Keyword(s):  
Antibacterial Activity ◽  
Essential Oils ◽  
Metallic Nanoparticles ◽  
Synergistic Effects ◽  
Medical Application ◽  
Colloidal Dispersion ◽  
Multidrug Resistant ◽  
Antimicrobial Activities ◽  
Promising Alternative

Silver nanoparticles (AgNPs) are considered a promising alternative to the use of antibiotics in fighting multidrug-resistant pathogens. However, their use in medical application is hindered by the public concern regarding the toxicity of metallic nanoparticles. In this study, rationally designed AgNP were produced, in order to balance the antibacterial activity and toxicity. A facile, environmentally friendly synthesis was used for the electrochemical fabrication of AgNPs. Chitosan was employed as the capping agent, both for the stabilization and to improve the biocompatibility. Size, morphology, composition, capping layer, and stability of the synthesized nanoparticles were characterized. The in vitro biocompatibility and antimicrobial activities of AgNPs against common Gram-negative and Gram-positive bacteria were evaluated. The results revealed that chitosan-stabilized AgNPs were nontoxic to normal fibroblasts, even at high concentrations, compared to bare nanoparticles, while significant antibacterial activity was recorded. The silver colloidal dispersion was further mixed with essential oils (EO) to increase the biological activity. Synergistic effects at some AgNP–EO ratios were observed, as demonstrated by the fractionary inhibitory concentration values. Our results reveal that the synergistic action of both polymer-stabilized AgNPs and essential oils could provide a significant efficiency against a large variety of microorganisms, with minimal side effects.

The Effect by Sodium Sulfite on the Enzyme Activity and Stabilization of Race Bran

2013 ◽  
Vol 634-638 ◽  
pp. 1219-1224 ◽  
Author(s):  
Guo Ping Yu ◽  
Zhu Gong ◽  
Man Xu Xu ◽  
Chao Ran Dou
Keyword(s):  
Enzyme Activity ◽  
Fatty Acid ◽  
Antioxidant Capacity ◽  
Optimal Condition ◽  
Rice Bran ◽  
Peroxide Value ◽  
Total Antioxidant Capacity ◽  
Sodium Sulfite ◽  
Total Antioxidant ◽  
The Stability

This paper focuses on the effect by sodium sulfite on the enzyme activity and stabilization of rice bran. First, enzymes are studied by detecting the activity of lipase and peroxidase. Then the total antioxidant capacity is tested in order to clarify the effect of sodium sulfite on antioxidant substances. Finally, processed rice bran is storaged in constant temperature to observe the change of fatty acid and peroxide value. The optimal condition is 4% sodium sulfite for 150min. The results show that the sodium sulfite can reduce the enzyme activity to improve the stability of rice bran.

scholarly journals Quantitative live-cell PALM reveals nanoscopic Faa4 redistributions and dynamics on lipid droplets during metabolic transitions of yeast

2021 ◽  
pp. mbc.E20-11-0695
Author(s):  
Santosh Adhikari ◽  
Joe Moscatelli ◽  
Elias M. Puchner
Keyword(s):  
Fatty Acid ◽  
Single Molecule ◽  
Lipid Droplets ◽  
Fold Increase ◽  
Yeast Cells ◽  
Exogenous Fatty Acid ◽  
Localization Microscopy ◽  
Fatty Acid Chain ◽  
Chain Lengths ◽  
Live Yeast

Lipid droplets (LDs) are dynamic organelles for lipid storage and homeostasis. Cells respond to metabolic changes by regulating the spatial distribution of LDs and enzymes required for LD growth and turnover. The small size of LDs precludes the observation of their associated enzyme densities and dynamics with conventional fluorescence microscopy. Here, we employ quantitative photo-activated localization microscopy to study the density of the fatty acid activating enzyme Faa4 on LDs in live yeast cells with single-molecule sensitivity and 30 nm resolution. During the log phase LDs co-localize with the Endoplasmic Reticulum (ER) where their emergence and expansion is mediated by the highest observed Faa4 densities. During transition to the stationary phase LDs with a ∼2-fold increased surface area translocate to the vacuolar surface and lumen and exhibit a ∼2.5-fold increase in Faa4 density. The increased Faa4 density on LDs further suggests its role in LD expansion, is caused by its ∼5-fold increased expression level and is specific to exogenous fatty acid chain-lengths. When lipolysis is induced by refreshed medium, Faa4 shuttles through ER- and lipophagy to the vacuole, where it may activate fatty acids for membrane expansion and degrade to reset cellular Faa4 abundance to levels in the log phase. [Media: see text] [Media: see text] [Media: see text] [Media: see text]

scholarly journals Evidence that the Essential Response Regulator YycF in Streptococcus pneumoniae Modulates Expression of Fatty Acid Biosynthesis Genes and Alters Membrane Composition

2005 ◽  
Vol 187 (7) ◽  
pp. 2357-2367 ◽  
Author(s):  
M. Luz Mohedano ◽  
Karin Overweg ◽  
Alicia de la Fuente ◽  
Mark Reuter ◽  
Silvia Altabe ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Streptococcus Pneumoniae ◽  
Fatty Acid Biosynthesis ◽  
Membrane Lipids ◽  
Response Regulator ◽  
Membrane Composition ◽  
Acid Biosynthesis ◽  
Fatty Acid Chain ◽  
Genome Wide ◽  
Chain Lengths

ABSTRACT The YycFG two-component system, originally identified in Bacillus subtilis, is highly conserved among gram-positive bacteria with low G+C contents. In Streptococcus pneumoniae, the YycF response regulator has been reported to be essential for cell growth, but the signal to which it responds and the gene members of the regulon remain unclear. In order to investigate the role of YycFG in S. pneumoniae, we increased the expression of yycF by using a maltose-inducible vector and analyzed the genome-wide effects on transcription and protein expression during the course of yycF expression. The induction of yycF expression increased histidine kinase yycG transcript levels, suggesting an autoregulation of the yycFG operon. Evidence from both proteomic and microarray transcriptome studies as well as analyses of membrane fatty acid composition indicated that YycFG is involved in the regulation of fatty acid biosynthesis pathways and in determining fatty acid chain lengths in membrane lipids. In agreement with recent transcriptome data on pneumococcal cells depleted of YycFG, we also identified several other potential members of the YycFG regulon that are required for virulence and cell wall biosynthesis and metabolism.

The Arabidopsis acyl-CoA oxidase gene family

2000 ◽  
Vol 28 (6) ◽  
pp. 755-757 ◽  
Author(s):  
P. J. Eastmond ◽  
M. Hooks ◽  
I. A. Graham
Keyword(s):  
Fatty Acid ◽  
Full Range ◽  
Biochemical Properties ◽  
Oxidase Gene ◽  
Substrate Specificities ◽  
Fatty Acid Chain ◽  
Acyl Coa Oxidase ◽  
Chain Lengths ◽  
Peroxisomal Fatty Acid

A family of acyl-CoA oxidase isozymes catalyse the first step in the peroxisomal fatty acid β-oxidation spiral. Our group and others have recently characterized four genes from this family in the model oilseed Arabidopsis. These genes encode isozymes with different acyl-CoA substrate specificities, which together encompass the full range of fatty acid chain lengths that exist in vivo. Here we review the biochemical properties and physiological roles of the acyl-CoA oxidase isozymes.

Satiation in humans is modulated by fatty acid chain length

2001 ◽  
Vol 120 (5) ◽  
pp. A710-A710
Author(s):  
S LAL ◽  
J MCLAUGHLIN ◽  
O NIAZ ◽  
G DOCKRAY ◽  
A VARRO ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Chain Length ◽  
Fatty Acid Chain Length ◽  
Fatty Acid Chain
Sign in / Sign up

Export Citation Format

Share Document