scholarly journals New Isolated Metschnikowia pulcherrima Strains from Apples for Postharvest Biocontrol of Penicillium expansum and Patulin Accumulation

Toxins ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 397
Author(s):  
Laura Settier-Ramírez ◽  
Gracia López-Carballo ◽  
Pilar Hernández-Muñoz ◽  
Angélique Fontana ◽  
Caroline Strub ◽  
...  
Keyword(s):  
Liquid Medium ◽  
Fungal Growth ◽  
Its Region ◽  
Antagonistic Activity ◽  
Penicillium Expansum ◽  
Metschnikowia Pulcherrima ◽  
Potential Activity ◽  
Different Strains ◽  
Main Producer

Wild yeasts isolated from the surface of apples were screened for antagonistic activity against Penicillium expansum, the main producer of the mycotoxin patulin. Three antagonistic yeasts (Y33, Y29 and Y24) from a total of 90 were found to inhibit P. expansum growth. Identification by ITS region sequence and characterization showed that three selected isolates of yeast should be different strains of Metschnikowia pulcherrima. Several concentrations of the selected yeasts were used to study their in vitro antifungal effectivity against P. expansum on Petri dishes (plates with 63.6 cm2 surface) whereas their potential activity on patulin reduction was studied in liquid medium. Finally, the BCA that had the best in vitro antifungal capacity against P. and the best patulin degradation capacity was selected to be assessed directly on apples. All the selected strains demonstrated antifungal activity in vitro but the most efficient was the strain Y29. Isolated strains were able to reduce patulin content in liquid medium, Y29 being the only strain that completely reduced patulin levels within 120 h. The application of Y29 as biocontrol agent on the surface of apples inoculated with P. expansum, inhibited fungal growth and patulin production during storage. Therefore, the results shown that this yeast strain could be used for the reduction of P. expansum and its mycotoxin in apples or apple-based products by adapting the procedure application.

scholarly journals Antifungal activity of several isolates of Trichoderma against Cladosporium and Botrytis

2018 ◽  
Vol 8 (1) ◽  
pp. 88-91 ◽  
Author(s):  
M. Skaptsov ◽  
S. Smirnov ◽  
M. Kutsev ◽  
O. Uvarova ◽  
T. Sinitsyna ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Trichoderma Harzianum ◽  
Its Region ◽  
Botanical Garden ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Soil Samples ◽  
Morphological Observation ◽  
The South

<p><em>Trichoderma</em> isolates (SSBGT07, SSBGT08, SSBGT09, SSBGT10) were isolated from the soil samples of the South-Siberian Botanical Garden and identified using morphological observation and ITS region analysis as <em>Trichoderma harzianum</em>, <em>T. asperellum, T. ghanense</em>, and <em>T. longibranchiatum</em>. Antagonistic activity against <em>Cladosporium </em>sp. and<em> Botrytis </em>sp. was evaluated <em>in vitro</em>. All isolates showed antagonistic effect by competition against <em>Cladosporium </em>sp. <em>T. asperellum </em>and <em>T. longibranchiatum</em> showed antagonism against <em>Botrytis </em>sp. All isolates showed hyper sporulation on the sclerotia of <em>Botrytis</em> sp. (except the <em>T. ghanense</em>) and colonies of the <em>Cladosporium</em> sp. Our study provides new isolates that affect the <em>Cladosporium </em>sp. and<em> Botrytis </em>sp.</p>

scholarly journals Pyrene and Chrysene Tolerance and Biodegradation Capability of Pleurotus Sajor-Caju

2018 ◽  
Vol 12 (1) ◽  
pp. 24-35 ◽  
Author(s):  
Giuliano Saiu ◽  
Stefania Tronci ◽  
Massimiliano Grosso ◽  
Enzo Cadoni ◽  
Nicoletta Curreli
Keyword(s):  
Liquid Medium ◽  
Copper Sulphate ◽  
Negative Impact ◽  
Fungal Growth ◽  
Tween 80 ◽  
White Rot ◽  
White Rot Fungus ◽  
Polycyclic Aromatic ◽  
Pleurotus Sajor Caju

Introduction: The present work focused on the biodegradation capability of a white-rot fungus, the Pleurotus sajor-caju, when exposed to polycyclic aromatic hydrocarbons. Methods: The research was carried out by using in vitro systems developed on Petri dishes, to evaluate the fungal tolerance to pyrene and chrysene, followed by experiments in liquid medium. The first experimental campaign was necessary to evaluate the conditions promoting fungal growth and tolerance (presence of surfactants, peptone, copper sulphate and lecithin) and it was designed and analysed using statistical techniques. Results: It was found that the fungal population growth is strongly inhibited by chrysene presence. On the other hand, pyrene had a mild negative impact on the mycelia growth, which seemed to be positively influenced by the presence of Tween 80 and copper sulphate. Starting from these results, the behaviour of Pleurotus sajor-caju in presence of pyrene was investigated in liquid medium. Results showed that the depletion of pyrene was evident during a period of 20 days, and removal efficiency was greater than 90%.

scholarly journals Human Antibodies against a Purified Glucosylceramide from Cryptococcus neoformans Inhibit Cell Budding and Fungal Growth

2000 ◽  
Vol 68 (12) ◽  
pp. 7049-7060 ◽  
Author(s):  
Marcio L. Rodrigues ◽  
Luiz R. Travassos ◽  
Kildare R. Miranda ◽  
Anderson J. Franzen ◽  
Sonia Rozental ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Cell Wall ◽  
Cryptococcus Neoformans ◽  
High Performance ◽  
Fungal Growth ◽  
Fungal Cell ◽  
Human Antibodies ◽  
Different Strains ◽  
Immunofluorescence Labeling

ABSTRACT A major ceramide monohexoside (CMH) was purified from lipidic extracts of Cryptococcus neoformans. This molecule was analyzed by high-performance thin-layer chromatography (HPTLC), gas chromatography coupled with mass spectrometry, and fast atom bombardment-mass spectrometry. The cryptococcal CMH is a β-glucosylceramide, with the carbohydrate residue attached to 9-methyl-4,8-sphingadienine in amidic linkage to 2-hydroxyoctadecanoic acid. Sera from patients with cryptococcosis and a few other mycoses reacted with the cryptococcal CMH. Specific antibodies were purified from patients' sera by immunoadsorption on the purified glycolipid followed by protein G affinity chromatography. The purified antibodies to CMH (mainly immunoglobulin G1) bound to different strains and serological types of C. neoformans, as shown by flow cytofluorimetry and immunofluorescence labeling. Transmission electron microscopy of yeasts labeled with immunogold-antibodies to CMH and immunostaining of isolated cell wall lipid extracts separated by HPTLC showed that the cryptococcal CMH predominantly localizes to the fungal cell wall. Confocal microscopy revealed that the β-glucosylceramide accumulates mostly at the budding sites of dividing cells with a more disperse distribution at the cell surface of nondividing cells. The increased density of sphingolipid molecules seems to correlate with thickening of the cell wall, hence with its biosynthesis. The addition of human antibodies to CMH to cryptococcal cultures of both acapsular and encapsulated strains of C. neoformans inhibited cell budding and cell growth. This process was complement-independent and reversible upon removal of the antibodies. The present data suggest that the cryptococcal β-glucosylceramide is a fungal antigen that plays a role on the cell wall synthesis and yeast budding and that antibodies raised against this component are inhibitory in vitro.

scholarly journals Role of Rhizobacteria Associated with Diseased Tomato Plants towards their Response with Ralstonia sp The Bacterial Wilt Agent

2020 ◽  
Vol 5 (4) ◽  
pp. 283-291
Author(s):  
Shraddha P. Mishra ◽  
Anfal Arshi ◽  
Ankur Agarwal ◽  
Merwyn P. Raj ◽  
Madhu Bala
Keyword(s):  
Bacterial Wilt ◽  
Agar Plate ◽  
In Vitro Study ◽  
Antagonistic Activity ◽  
Bacterial Strains ◽  
Tomato Crop ◽  
Bacterial Wilt Disease ◽  
Ralstonia Pseudosolanacearum ◽  
Different Strains

In this study, we have aimed to determine the antagonistic effects of various rhizobacteria against representatives of bacterial wilt disease; Ralstonia pseudosolanacearum with a comparison from control sterile distilled water (SDW) aliquots. During in-vitro study, bacterial wilt agent was clearly inhibited in their growth by significant percentage by 32 different strains through perforated agar plate method. Results showed 10 potential strains among those 32 strains viz. DIB76/BCS-8, DIB76/BCS-9, DIB76/BCS-10, DIB76/BCS-12, DIB76/BCS-19, DIB76/BCS-20, DIB76/BCS-21, DIB76/BCS-24, DIB76/BCS-26 and DIB76/BCS-27 had the highest growth suppression for 9 different strains of R. pseudosolanacearum as compared to other bacterial strains. Trials with tomato crop at polyhouse had no incidence of bacterial wilt than those for control. Study, thus identifies 10 rhizobacterial strains from rhizosphere soil of diseased tomato plant possessing potential antagonistic activity against the wilt pathogen and has prospects as good biocontrol agents.

scholarly journals Exploring the Biodiversity of Red Yeasts for In Vitro and In Vivo Phenotypes Relevant to Agri-Food-Related Processes

Fermentation ◽  
2020 ◽  
Vol 7 (1) ◽  
pp. 2
Author(s):  
Giacomo Zara ◽  
Maria Grazia Farbo ◽  
Chiara Multineddu ◽  
Quirico Migheli ◽  
Marilena Budroni ◽  
...  
Keyword(s):  
Apple Fruit ◽  
Antagonistic Activity ◽  
Penicillium Expansum ◽  
Quantitative Composition ◽  
Invasive Growth ◽  
Phenotypic Data ◽  
Plastic Materials ◽  
Fruit Decay

Red yeasts grow on food wastes, show antagonistic activity against food-spoilage microorganisms, produce food supplements, and may be utilized as feed-supplements themselves to positively modulate the quali-quantitative composition of intestinal microbiota. Therefore, they show a variety of possible biotechnological applications in agri-food-related processes. Here, to further explore the biotechnological potential of red yeasts, eleven strains ascribed to different species of the genera Rhodotorula and Sporobolomyces, differing for biomass and carotenoids production, were characterized in vitro for biofilm formation, invasive growth, and growth at the temperature range of 20–40 °C and in vivo for their antagonistic activity against the fungal pathogen and patulin producer Penicillium expansum. Most of them formed cellular MAT and showed invasive growth as well as adhesion to plastic materials. Four strains determined a significant reduction of fruit decay caused by P. expansum on apple fruit while the remaining seven showed different degrees of biocontrol activity. Finally, none of them grew at body temperature (>37 °C). Statistical analyses of both qualitative and quantitative phenotypic data, including biomass and carotenoids production, gathered further information on the most interesting strains for the biotechnological exploitation of red yeasts in agri-food-related process.

scholarly journals Effects of Some Polyamine Biosynthesis Inhibitors and Calcium Chloride on In Vitro Growth and Decay Development in Apples Caused by Botrytis cinerea and Penicillium expansum

1997 ◽  
Vol 122 (3) ◽  
pp. 380-385 ◽  
Author(s):  
Robert A. Saftner ◽  
William S. Conway ◽  
Carl E. Sams
Keyword(s):  
Botrytis Cinerea ◽  
Fungal Growth ◽  
Soft Rot ◽  
Penicillium Expansum ◽  
In Vitro Growth ◽  
Polyamine Biosynthesis ◽  
Pressure Infiltration ◽  
Biosynthesis Inhibitors ◽  
Decay Development

Three polyamine biosynthesis inhibitors, α-difluoromethylornithine (DFMO), α-difluoromethylarginine (DFMA), and α-methylornithine (MeOrn), alone and in combination with CaCl2, were tested for their ability to reduce in vitro growth and soft rot development in apple (Malus domestica Borkh.) fruit caused by Botrytis cinerea Pers.:Fr. and Penicillium expansum Link. All three inhibitors reduced the in vitro growth of the pathogens. Calcium had no effect on fungal growth in vitro. Pressure infiltration of millimolar concentrations of DFMO or DFMA or 25 g·L-1 CaCl2 solutions into apples reduced subsequent soft rot development by B. cinerea and P. expansum >40%. A combination treatment of Ca and DFMO or DFMA reduced decay >67%. Treatment of apples with MeOrn was less effective at inhibiting decay development. None of the inhibitors affected polyamine levels in apple cortical tissues. Some injury to the fruit surface was observed with Ca treatments. Fruit treated with Ca and any of the inhibitors were less firm than those treated with Ca alone. Specific polyamine biosynthesis inhibitors in combination with Ca may prove useful in reducing postharvest decay in apples.

ASSESSMENT OF SOME ABIOTIC FACTORS ON MICROBIAL BIOPRODUCTS USEFUL IN BIOCONTROL OF PHYTOPATHOGENS

2016 ◽  
Vol 5 (3) ◽  
pp. 799-803
Author(s):  
Sergiu Fendrihan ◽  
Sorina Dinu ◽  
Oana Alina Sicuia ◽  
Florica Constantinescu
Keyword(s):  
Abiotic Factors ◽  
Fungal Growth ◽  
Phytopathogenic Fungi ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Limiting Factors ◽  
Multiplication Rate ◽  
Antifungal Metabolites ◽  
Ph Conditions

The environmental factors may influence the growth of microorganisms, by favoring their growth or slowing their multiplication rate and the synthesis of different metabolites. Parameters such as temperature, aeration, nutrients, pH or tolerance to NaCl can become limiting factors for microorganisms survival. Bacillus subtilis and related species can grow in variable pH conditions, maintaining the cytoplasmically pH in a relatively close range, stable to the synthesis of proteins and nucleic acids.The aim of this work was to assess the influence of some abiotic factors on the biocontrol activity of microbial bioproducts, based on beneficial strains from Bacillus sp.. The bioproducts were tested in vitro against soil borne fungi at different temperatures and pH conditions. The results showed that the antagonistic activity of the biopreparates, tested at 27°C and 25°C, against phytopathogenic fungi released antifungal metabolites which inhibited the fungal growth. Also, when different pH values were analyzed, the results reflected that at pH 5.5 and pH 8.5 the bioproducts maintained the same antagonistic effect as in the control variant (pH 7.0).

scholarly journals Resistance of Postharvest Biocontrol Yeasts to Oxidative Stress: A Possible New Mechanism of Action

2003 ◽  
Vol 93 (5) ◽  
pp. 564-572 ◽  
Author(s):  
Raffaello Castoria ◽  
Leonardo Caputo ◽  
Filippo De Curtis ◽  
Vincenzo De Cicco
Keyword(s):  
Oxidative Stress ◽  
Superoxide Anion ◽  
Rhodotorula Glutinis ◽  
Antagonistic Activity ◽  
Model System ◽  
Penicillium Expansum ◽  
Cryptococcus Laurentii ◽  
Oxygen Species ◽  
Combined Application

We detected the generation of the reactive oxygen species (ROS) superoxide anion ( O·- 2) and hydrogen peroxide (H2O2) in apple wounds 2 immediately after wounding, and assessed the relationships between (i) timely colonization of apple wounds by biocontrol yeasts, (ii) resistance of these microorganisms to oxidative stress caused by ROS, and (iii) their antagonism against postharvest wound pathogens. We analyzed a model system consisting of two yeasts with higher (Cryptococcus laurentii LS-28) or lower (Rhodotorula glutinis LS-11) antagonistic activity against the postharvest pathogens Botrytis cinerea and Penicillium expansum. LS-28 exhibited faster and greater colonization of wounds than LS-11. In contrast to LS-28, the number of LS-11 cells dropped 1 and 2 h after application, and then increased only later. In vitro, LS-28 was more resistant to ROS-generated oxidative stress. The combined application of biocontrol yeasts and ROS-deactivating enzymes in apple wounds prevented the decrease in number of LS-11 cells mentioned above, and enhanced colonization and antagonistic activity of both biocontrol yeasts against B. cinerea and P. expansum. Polar lipids of LS-11 contained the more unsaturated and oxidizable α-linolenic acid, which was absent in LS-28. Resistance to oxidative stress could be a key mechanism of biocontrol yeasts antagonism against postharvest wound pathogens.

scholarly journals In Vitro Antagonistic Activity of Diverse Bacillus Species Against Fusarium culmorum and F. solani Pathogens

2020 ◽  
Vol 14 (1) ◽  
pp. 157-163
Author(s):  
M. Harba ◽  
M. Jawhar ◽  
M.I.E. Arabi
Keyword(s):  
Fungal Pathogens ◽  
Fusarium Culmorum ◽  
Antagonistic Activity ◽  
Broth Culture ◽  
Bacillus Species ◽  
Rrna Gene ◽  
Antagonistic Effects ◽  
Biological Potential ◽  
Potential Activity

Background: Fusarium culmorum and Fusarium solani are economically important fungal pathogens of many plant species causing significant yield losses worldwide. Frequent uses of fungicides are hazardous to humans and the environment. Therefore, in vitro antagonistic activity of diverse Bacillus species isolates with biological potential activity to control these both pathogens should be investigated. Objective: The objectives were to isolate and identify the Bacillus spp., which are potential controls of F. culmorum and F. solani, and to characterize molecularly, at the species level, those isolates that have potential as biocontroller of the pathogens. Methods: The in vitro antagonistic potential of 40 Bacillus isolates against F. culmorum and F. solani was evaluated on the basis of fungal growth inhibition on nutrient broth culture. The colony morphology and the 16S rRNA gene sequencing of Bacillus spp. were used to identify the isolates. Results: Bacillus sp. isolates were identified as B. atrophaeus, B. subtilis, Paenibacillus polymxa, B. amyloliquefaciens, B. simplex and B. tequilensis. They had significant (P<0.05) antagonistic activities against F. culmorum and F. solani isolates as compared to the untreated control. The antagonistic effects varied depending on the Fusarium sp. The bacterial B. subtilis isolates SY116C and SY SY118C provided the most noteworthy result as both strongly inhibited mycelial growth of F. solani by 97.2%, while the B. tequilensis isolate SY145D was the most effective in the formation of inhibition zones against F. culmorum by 75%. Conclusion: It is apparent that Bacillus sp. isolates play an important role in the inhibition of growth of F. culmorum and F. solani, and that the B. subtilis isolates SY116C and SY118C had the highest biological potential activity against these fungi. These antagonistic effects may be important contributors as a biocontrol approach that could be employed as a part of integrated soil pathogen management system.

Effect of the endophytic plant growth promoting Enterobacter ludwigii EB4B on tomato growth

2020 ◽  
Vol 13 (2) ◽  
pp. 54-65 ◽  
Author(s):  
M.E.A. Bendaha ◽  
H.A. Belaouni
Keyword(s):  
Root Length ◽  
Biocontrol Agent ◽  
Growth Promotion ◽  
Dry Weight ◽  
Antagonistic Activity ◽  
Rrna Gene ◽  
Plant Growth Promoting ◽  
Enterobacter Ludwigii ◽  
Tomato Growth

SummaryThis study aims to develop a biocontrol agent against Fusarium oxysporum f.sp. radicis-lycopersici (FORL) in tomato. For this, a set of 23 bacterial endophytic isolates has been screened for their ability to inhibit in vitro the growth of FORL using the dual plate assay. Three isolates with the most sound antagonistic activity to FORL have been qualitatively screened for siderophore production, phosphates solubilization and indolic acetic acid (IAA) synthesis as growth promotion traits. Antagonistic values of the three candidates against FORL were respectively: 51.51 % (EB4B), 51.18 % (EB22K) and 41.40 % (EB2A). Based on 16S rRNA gene sequence analysis, the isolates EB4B and EB22K were closely related to Enterobacter ludwigii EN-119, while the strain EB2A has been assigned to Leclercia adecarboxylata NBRC 102595. The promotion of tomato growth has been assessed in vitro using the strains EB2A, EB4B and EB22K in presence of the phytopathogen FORL. The treatments with the selected isolates increased significantly the root length and dry weight. Best results were observed in isolate EB4B in terms of growth promotion in the absence of FORL, improving 326.60 % of the root length and 142.70 % of plant dry weight if compared with untreated controls. In the presence of FORL, the strain EB4B improved both root length (180.81 %) and plant dry weight (202.15 %). These results encourage further characterization of the observed beneficial effect of Enterobacter sp. EB4B for a possible use as biofertilizer and biocontrol agent against FORL.

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