scholarly journals A Combined Transcriptomics and Proteomics Approach Reveals the Differences in the Predatory and Defensive Venoms of the Molluscivorous Cone Snail Cylinder ammiralis (Caenogastropoda: Conidae)

Toxins ◽  
2021 ◽  
Vol 13 (9) ◽  
pp. 642
Author(s):  
Samuel Abalde ◽  
Sébastien Dutertre ◽  
Rafael Zardoya
Keyword(s):  
Relative Abundance ◽  
Venom Gland ◽  
Complex Mixtures ◽  
High Expression ◽  
Cone Snail ◽  
Protein Families ◽  
Animal Kingdom ◽  
Expression Levels ◽  
Proteomics Approach ◽  
First Time

Venoms are complex mixtures of proteins that have evolved repeatedly in the animal kingdom. Cone snail venoms represent one of the best studied venom systems. In nature, this venom can be dynamically adjusted depending on its final purpose, whether to deter predators or hunt prey. Here, the transcriptome of the venom gland and the proteomes of the predation-evoked and defensive venoms of the molluscivorous cone snail Cylinder ammiralis were catalogued. A total of 242 venom-related transcripts were annotated. The conotoxin superfamilies presenting more different peptides were O1, O2, T, and M, which also showed high expression levels (except T). The three precursors of the J superfamily were also highly expressed. The predation-evoked and defensive venoms showed a markedly distinct profile. A total of 217 different peptides were identified, with half of them being unique to one venom. A total of 59 peptides ascribed to 23 different protein families were found to be exclusive to the predatory venom, including the cono-insulin, which was, for the first time, identified in an injected venom. A total of 43 peptides from 20 protein families were exclusive to the defensive venom. Finally, comparisons of the relative abundance (in terms of number of peptides) of the different conotoxin precursor superfamilies showed that most of them present similar abundance regardless of the diet.

scholarly journals STEP® vectors for rapid generation of stable transfected CHO cell pools and clones with high expression levels and product quality homogeneity of difficult-to-express proteins

2021 ◽  
pp. 105920
Author(s):  
Abhinav Luthra ◽  
Remco A. Spanjaard ◽  
Sarwat Cheema ◽  
Nathalie Veith ◽  
Lars Kober ◽  
...  
Keyword(s):  
Product Quality ◽  
High Expression ◽  
Cho Cell ◽  
Expression Levels ◽  
Rapid Generation

scholarly journals Venom-gland transcriptomic, venomic, and antivenomic profiles of the spine-bellied sea snake (Hydrophis curtus) from the South China Sea

BMC Genomics ◽  
2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Hong-Yan Zhao ◽  
Lin Wen ◽  
Yu-Feng Miao ◽  
Yu Du ◽  
Yan Sun ◽  
...  
Keyword(s):  
South China Sea ◽  
South China ◽  
Evolutionary History ◽  
De Novo ◽  
Venom Gland ◽  
The South China Sea ◽  
Protein Families ◽  
The South ◽  
Widespread Species ◽  
China Sea

Abstract Background A comprehensive evaluation of the -omic profiles of venom is important for understanding the potential function and evolution of snake venom. Here, we conducted an integrated multi-omics-analysis to unveil the venom-transcriptomic and venomic profiles in a same group of spine-bellied sea snakes (Hydrophis curtus) from the South China Sea, where the snake is a widespread species and might generate regionally-specific venom potentially harmful to human activities. The capacity of two heterologous antivenoms to immunocapture the H. curtus venom was determined for an in-depth evaluation of their rationality in treatment of H. curtus envenomation. In addition, a phylogenetic analysis by maximum likelihood was used to detect the adaptive molecular evolution of full-length toxin-coding unigenes. Results A total of 90,909,384 pairs of clean reads were generated via Illumina sequencing from a pooled cDNA library of six specimens, and yielding 148,121 unigenes through de novo assembly. Sequence similarity searching harvested 63,845 valid annotations, including 63,789 non-toxin-coding and 56 toxin-coding unigenes belonging to 22 protein families. Three protein families, three-finger toxins (3-FTx), phospholipase A2 (PLA2), and cysteine-rich secretory protein, were detected in the venom proteome. 3-FTx (27.15% in the transcriptome/41.94% in the proteome) and PLA2 (59.71%/49.36%) were identified as the most abundant families in the venom-gland transcriptome and venom proteome. In addition, 24 unigenes from 11 protein families were shown to have experienced positive selection in their evolutionary history, whereas four were relatively conserved throughout evolution. Commercial Naja atra antivenom exhibited a stronger capacity than Bungarus multicinctus antivenom to immunocapture H. curtus venom components, especially short neurotoxins, with the capacity of both antivenoms to immunocapture short neurotoxins being weaker than that for PLA2s. Conclusions Our study clarified the venom-gland transcriptomic and venomic profiles along with the within-group divergence of a H. curtus population from the South China Sea. Adaptive evolution of most venom components driven by natural selection appeared to occur rapidly during evolutionary history. Notably, the utility of commercial N. atra and B. multicinctus antivenoms against H. curtus toxins was not comprehensive; thus, the development of species-specific antivenom is urgently needed.

scholarly journals Bioinformatics study on genes related to a high-risk postoperative recurrence of lung adenocarcinoma

2021 ◽  
Vol 104 (3) ◽  
pp. 003685042110180
Author(s):  
Xiao Lin ◽  
Meng Zhou ◽  
Zehong Xu ◽  
Yusheng Chen ◽  
Fan Lin
Keyword(s):  
Cell Cycle ◽  
Gene Ontology ◽  
High Risk ◽  
Lung Adenocarcinoma ◽  
Candidate Genes ◽  
Postoperative Recurrence ◽  
High Expression ◽  
Ppi Network ◽  
Hub Genes ◽  
Expression Levels

In this study, we aimed to screen out genes associated with a high risk of postoperative recurrence of lung adenocarcinoma and investigate the possible mechanisms of the involvement of these genes in the recurrence of lung adenocarcinoma. We identify Hub genes and verify the expression levels and prognostic roles of these genes. Datasets of GSE40791, GSE31210, and GSE30219 were obtained from the Gene Expression Omnibus database. Enrichment analysis of gene ontology and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were performed for the screened candidate genes using the DAVID database. Then, we performed protein–protein interaction (PPI) network analysis through the database STRING. Hub genes were screened out using Cytoscape software, and their expression levels were determined by the GEPIA database. Finally, we assessed the relationships of Hub genes expression levels and the time of survival. Forty-five candidate genes related to a high-risk of lung adenocarcinoma recurrence were screened out. Gene ontology analysis showed that these genes were enriched in the mitotic spindle assembly checkpoint, mitotic sister chromosome segregation, G2/M-phase transition of the mitotic cell cycle, and ATP binding, etc. KEGG analysis showed that these genes were involved predominantly in the cell cycle, p53 signaling pathway, and oocyte meiosis. We screened out the top ten Hub genes related to high expression of lung adenocarcinoma from the PPI network. The high expression levels of eight genes (TOP2A, HMMR, MELK, MAD2L1, BUB1B, BUB1, RRM2, and CCNA2) were related to short recurrence-free survival and they can be used as biomarkers for high risk of lung adenocarcinoma recurrence. This study screened out eight genes associated with a high risk of lung adenocarcinoma recurrence, which might provide novel insights into researching the recurrence mechanisms of lung adenocarcinoma as well as into the selection of targets in the treatment of the disease.

scholarly journals High expression levels of egfl7 correlate with low endothelial cell activation in peritumoral vessels of human breast cancer

2016 ◽  
Vol 12 (2) ◽  
pp. 1422-1428 ◽  
Author(s):  
Diane Pannier ◽  
Géraldine Philippin-Lauridant ◽  
Marie-Christine Baranzelli ◽  
Delphine Bertin ◽  
Emilie Bogart ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Endothelial Cell ◽  
Human Breast Cancer ◽  
Cell Activation ◽  
High Expression ◽  
Human Breast ◽  
Endothelial Cell Activation ◽  
Expression Levels ◽  
Peritumoral Vessels

scholarly journals A strategy to obtain recombinant cell lines with high expression levels. Lentiviral vector-mediated transgenesis

2011 ◽  
Vol 5 (S8) ◽  
Author(s):  
Claudio Prieto ◽  
Diego Fontana ◽  
Marina Etcheverrigaray ◽  
Ricardo Kratje
Keyword(s):  
Cell Lines ◽  
Lentiviral Vector ◽  
High Expression ◽  
Expression Levels ◽  
Recombinant Cell

scholarly journals VII.—Foraminifera from the London Clay of Sheppey

1889 ◽  
Vol 6 (11) ◽  
pp. 498-499 ◽  
Author(s):  
Frederick Chapman ◽  
C. Davies Sherborn
Keyword(s):  
Geographical Distribution ◽  
Relative Abundance ◽  
London Clay ◽  
First Time

In the Proceedings of the Geologists' Association for 1878, Mr. W. H. Shrubsole, F.G.S., published a list of Foraminifera obtained from the London Clay of Sheppey. The following list, the result of an examination of some material courteously lent to us by Professor J. W. Judd, F.R.S., adds considerably to the fauna of Sheppey and includes two species not previously recorded from the London Clay. Forty-one forms have been determined, of which twenty-six. are new to Sheppey, thus bringing up the number of forms recorded from that locality to eighty-six. The geographical distribution of the Foraminifera of the London Clay was fully tabulated in 1886, and it is interesting to find so many of the forms there figured and recorded for the first time from the London area common to both localities. The figure following the specific name in the list appended shows the relative abundance of the varieties found.

Identification of a Novel Family of Snake Venom Proteins Veficolins fromCerberus rynchopsUsing a Venom Gland Transcriptomics and Proteomics Approach

2010 ◽  
Vol 9 (4) ◽  
pp. 1882-1893 ◽  
Author(s):  
G. OmPraba ◽  
Alex Chapeaurouge ◽  
Robin Doley ◽  
K. Rama Devi ◽  
P. Padmanaban ◽  
...  
Keyword(s):  
Snake Venom ◽  
Venom Gland ◽  
Proteomics Approach ◽  
Venom Proteins

scholarly journals Identification of Type II Interferon Receptors in Geese: Gene Structure, Phylogenetic Analysis, and Expression Patterns

2015 ◽  
Vol 2015 ◽  
pp. 1-14
Author(s):  
Hao Zhou ◽  
Shun Chen ◽  
Yulin Qi ◽  
Qin Zhou ◽  
Mingshu Wang ◽  
...  
Keyword(s):  
Expression Profiles ◽  
Expression Patterns ◽  
Harderian Gland ◽  
Distribution Analysis ◽  
Expression Levels ◽  
Age Related ◽  
Cecal Tonsil ◽  
Immune Tissues ◽  
First Time

Interferonγreceptor 1 (IFNGR1) and IFNGR2 are two cell membrane molecules belonging to class II cytokines, which play important roles in the IFN-mediated antiviral signaling pathway. Here, goose IFNGR1 and IFNGR2 were cloned and identified for the first time. Tissue distribution analysis revealed that relatively high levels of goose IFNγmRNA transcripts were detected in immune tissues, including the harderian gland, cecal tonsil, cecum, and thymus. Relatively high expression levels of both IFNGR1 and IFNGR2 were detected in the cecal tonsil, which implicated an important role of IFNγin the secondary immune system of geese. No specific correlation between IFNγ, IFNGR1, and IFNGR2 expression levels was observed in the same tissues of healthy geese. IFNγand its cognate receptors showed different expression profiles, although they appeared to maintain a relatively balanced state. Furthermore, the agonist R848 led to the upregulation of goose IFNγbut did not affect the expression of goose IFNGR1 or IFNGR2. In summary, trends in expression of goose IFNγand its cognate receptors showed tissue specificity, as well as an age-related dependency. These findings may help us to better understand the age-related susceptibility to pathogens in birds.

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