scholarly journals Development of a Simple Single-Acupoint Electroacupuncture Frame and Evaluation of the Acupuncture Effect in Rabbits

2021 ◽  
Vol 8 (10) ◽  
pp. 217
Author(s):  
Huan Huang ◽  
Jianrong Zhang ◽  
Fuxing Gui ◽  
Sheng Liu ◽  
Chonghua Zhong ◽  
...  
Keyword(s):  
Creatine Kinase ◽  
High Frequency ◽  
The Body ◽  
Control Group ◽  
Output Current ◽  
Blood Samples ◽  
Time Points ◽  
Creatine Kinase Mb ◽  
Acupuncture Effect ◽  
New Zealand Rabbits

To reduce the circulation path of the output current of traditional electroacupuncture (TEA) process in the body, a simple single-acupoint electroacupuncture (SEA) frame was designed and the acupuncture effect of SEA was evaluated through Hou-san-li (ST-36) and Qian-san-li (LI-10) acupoints. Forty-two healthy New Zealand rabbits were randomly divided into seven groups and underwent acupuncture for 20 min in an awake state. Blood samples aseptically collected from the ear vein 3 h before acupuncture and 0, 3, 6, 9, 12 and 24 h after acupuncture were used for the detection of aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatine kinase MB (CK-MB) and motilin (MTL) in serum. The simple SEA frame was developed successfully, and the acupuncture results showed that the serum AST and ALT levels were significantly higher at 3 h after TEA with high frequency (p < 0.01) compared with the control group. Regarding serum CK-MB levels, no significant differences were found after SEA or TEA stimulation (p > 0.05). Serum MTL levels were significantly increased at 0 h after SEA and TEA (p < 0.05), but there were no significant differences at other time points after SEA and TEA treatment (p > 0.05). SEA not only maintains the effect of TEA but also shortens the circulation loop of the electroacupuncture (EA) current in the body, which effectively avoids body injury.

scholarly journals Serum Protein Profile Of Iraqi Hydatidosis Patients with Different Sites of Infection

2007 ◽  
Vol 4 (4) ◽  
pp. 612-616
Author(s):  
Baghdad Science Journal
Keyword(s):  
Serum Protein ◽  
Protein Profile ◽  
The Body ◽  
The Other ◽  
Control Group ◽  
Healthy Individuals ◽  
Banding Patterns ◽  
Blood Samples ◽  
Molecular Weights ◽  
Serum Protein Profile

Blood samples of One hundred and twenty patients from different hospitals in Baghdad infected with hydatidosis in different sites of the body (Liver, Lung, multiorgans and kidney) were collected for this study. On the other hand, 30 healthy individuals were included as a control group. This study was conducted to evaluate the effect of this disease on the serum protein profile of the patients using electrophoresis. The results revealed four different protein banding patterns with difference in number of bands and their molecular weights in comparison to the control group, and these differences depended on the site of infection. However the data showed a presence of the same band in all patients with different site of infection.

scholarly journals The Effects of Formaldehyde on Cytochrome P450 Isoform Activity in Rats

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Min Xu ◽  
Huaqiao Tang ◽  
Qian Rong ◽  
Yuanli Zhang ◽  
Yinglun Li ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Plasma Concentrations ◽  
Pharmacokinetic Parameters ◽  
Control Group ◽  
High Dose ◽  
Blood Samples ◽  
Time Points ◽  
Indoor Pollutant ◽  
Carcinogenic Effects

Formaldehyde (FA) is an occupational and indoor pollutant. Long-term exposure to FA can irritate the respiratory mucosa, with potential carcinogenic effects on the airways. The effects of acute FA poisoning on the activities of CYP450 isoforms CYP1A2, CYP2C11, CYP2E1, and CYP3A2 were assessed by determining changes in the pharmacokinetic parameters of the probe drugs phenacetin, tolbutamide, chlorzoxazone, and testosterone, respectively. Rats were randomly divided into three groups: control, low FA dose (exposure to 110 ppm for 2 h for 3 days), and high FA dose (exposure to 220 ppm for 2 h for 3 days). A mixture of the four probe drugs was injected into rats and blood samples were taken at a series of time points. Plasma concentrations of the probe drugs were measured by HPLC. The pharmacokinetic parameters t1/2, AUC(0-t), and Cmax of tolbutamide, chlorzoxazone, and testosterone increased significantly in the high dose versus control group (P<0.05), whereas the CL of chlorzoxazone and testosterone decreased significantly (P<0.05). However, t1/2, AUC(0-t), and Cmax of phenacetin decreased significantly (P<0.05), whereas the CL of phenacetin increased significantly (P<0.05) compared to controls. Thus, acute FA poisoning suppressed the activities of CYP2C11, CYP2E1, and CYP3A2 and induced the activity of CYP1A2 in rats. And the change of CYP450 activity caused by acute FA poisoning may be associated with FA potential carcinogenic effects on the airways.

scholarly journals Influence of Gender on Total Serum Creatine Kinase and Creatine Kinase-Mb Levels of Nigerian Hypertensives

2021 ◽  
Vol 6 (2) ◽  
pp. 1436-1439
Author(s):  
Henry Afamuefuna Efobi ◽  
Iya Eze Bassey
Keyword(s):  
Blood Pressure ◽  
Body Mass Index ◽  
Creatine Kinase ◽  
Body Mass ◽  
The Body ◽  
Total Serum ◽  
Creatine Kinase Mb ◽  
Normotensive Subjects ◽  
Total Creatine ◽  
Gender Specific

Introduction: Hypertension is a long-term medical condition which when not detected and managed properly and on time often results to complications leading to disabilities and mortality. Gender has been known to affect the interpretation of some variables necessitating the need for gender-specific ranges. Objectives:This study evaluated total creatine kinase (CK) and creatine kinase-MB (CK-MB) isoenzyme activities of hypertensive subjects in Calabar, Nigeria and to assess if gender has influence on the activities of these enzymes. Methodology:One hundred and two participants were consecutively enrolled in this case control study. Serum total serum CK and CK-MB activity were evaluated in fifty one hypertensive and 51 normotensive subjects. The total CK levels were assessed using a spectrophotometric method while immunoinhibition method was used to determine the activity of CK-MB. Data was analyzed using Student’s t-test and Pearson’s correlation. Statistical significance was set at p<0.05. Results:The total creatine kinase activities of the hypertensives did not differ significantly from those of the normotensive controls (p = 0.428) while the serum CK-MB activities of the hypertensive subjects were significantly higher than those of the normotensive controls (p=0.000). The body mass index of the hypertensives was significantly higher than those of the normotensive subjects (p=0.030). Gender had no effect on the blood pressure, body mass index and levels of CK and CK-MB (p>0.05). There was also no significant correlation (p>0.05) between blood pressure, body mass index and the levels of CK and CK-MB. Conclusion:CK-MB activities were significantly higher in hypertensive subjects compared to normotensive controls. There were no gender specific differences in the CK-MB levels of male and female hypertensives. This cardiac marker should be included in the routine assessment of hypertensives and gender-specific considerations may not be necessary in the interpretation of the data.

scholarly journals Study on the effect of intracoronary Adenosine on the incidence of myonecrosis after elective percutaneous coronary intervention

2009 ◽  
Vol 4 (2) ◽  
pp. 19-23
Author(s):  
Md Razib Ahsan ◽  
Md Abu Siddique ◽  
KMHS Sirajul Haque ◽  
Syed Ali Ahsan ◽  
Md Khurshed Ahmed ◽  
...  
Keyword(s):  
Percutaneous Coronary Intervention ◽  
Creatine Kinase ◽  
Case Control Study ◽  
Coronary Intervention ◽  
Control Group ◽  
Case Group ◽  
Elective Percutaneous Coronary Intervention ◽  
Percutaneous Coronary ◽  
Creatine Kinase Mb ◽  
Control Study

Appearance of serum creatine kinase MB fraction (CK-MB), reflecting the periprocedural myonecrosis, after successful percutaneous coronary intervention (PCI) is associated with future cardiac adverse events. The intracoronary adenosine on the incidence of myonecrosis after elective PCI was assessed. In this prospective, case control study with a total of 60 patients, 30 cases pretreated with 50 micro grams bolus intracoronary adenosine before advancement of guidewire and 30 controls not pretreated was studied during elective PCI. Both groups received statin and loading dose of Clopidogrel. Myonecrosis was assessed by CK-MB level after PCI. Post procedural myonecrosis occurred in 16.7 % (n=5) in case group and 36.7 % (n=11) in control group (OR=1.818; 95% CI 0.841; P=0.080). The result is not statistically significant but shows that the incidence of myonecrosis was reduced from 36.7% to 16.7%. Intracoronary adenosine reduces the incidence of myonecrosis after elective PCI. Keywords: Adenosine; Creatine kinase MB fraction; Intracoronary; Myonecrosis; Percutaneous coronary intervention; Periprocedural.   doi:10.3329/uhj.v4i2.2068 University Heart Journal Vol. 4 No. 2 July 2008 p19-23

scholarly journals Antioxidant and Anti-Inflammatory Effects of Curcumin Nanoparticles on Drug-Induced Acute Myocardial Infarction in Diabetic Rats

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 504 ◽  
Author(s):  
Boarescu ◽  
Boarescu ◽  
Bocșan ◽  
Gheban ◽  
Bulboacă ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Myocardial Infarction ◽  
Acute Myocardial Infarction ◽  
Creatine Kinase ◽  
Serum Levels ◽  
Control Group ◽  
Protective Effects ◽  
Curcumin Nanoparticles ◽  
Creatine Kinase Mb ◽  
Anti Inflammatory

We have investigated the cardio-protective effects of pretreatment with curcumin nanoparticles (CUN) compared to conventional curcumin (CUS) on the changes in oxidative stress parameters and inflammatory cytokine levels during induced acute myocardial infarction (AMI) in rats with diabetes mellitus (DM). DM was induced with streptozotocin, and AMI with isoproterenol. Eight groups of seven Wister Bratislava rats were included in the study. The N-C was the normal control group, AMI-C was the group with AMI, DM-C was the group with DM, and DM-AMI-C was the group with DM and AMI. All four groups received saline solution orally during the whole experiment. S-DM-CUS-AMI and S-DM-CUN-AMI groups received saline for seven days prior to DM induction and continued with CUS (200 mg/kg bw, bw = body weight) for S-DM-CUS-AMI and CUN for S-DM-CUN-AMI (200 mg/kg bw) for 15 days before AMI induction. The CUS-DM-CUS-AMI group received CUS (200 mg/kg bw), while the CUN-DM-CUN-AMI received CUN (200 mg/kg bw) for seven days prior to DM induction, and both groups continued with administration in the same doses for 15 days before AMI induction. CUS and CUN prevented elevation of creatine kinase, creatine kinase-MB, lactate dehydrogenase in all groups, with better results in the CUN (S-DM-CUN-AMI and CUN-DM-CUN-AMI groups). CUS and CUN significantly reduced serum levels of oxidative stress markers (malondialdehyde, the indirect assessment of nitric oxide synthesis, and total oxidative status) and enhanced antioxidative markers (total antioxidative capacity and thiols, up to 2.5 times). All groups that received CUS or CUN showed significantly lower serum levels of tumor necrosis factor-alpha, interleukin-6, and interleukin-1β. The best antioxidative and anti-inflammatory effects were obtained for the group that received CUN before DM induction (CUN-DM-CUN-AMI group). Pretreatment with CUN proved higher cardio-protective effects exerting an important antioxidative and anti-inflammatory impact in the case of AMI in DM.

A comparison of cardiac post-conditioning and remote pre-conditioning in paediatric cardiac surgery

2011 ◽  
Vol 21 (3) ◽  
pp. 266-270 ◽  
Author(s):  
Wanjun Luo ◽  
Ming Zhu ◽  
Rimao Huang ◽  
Yangde Zhang
Keyword(s):  
Creatine Kinase ◽  
Cardiac Surgery ◽  
Cardiopulmonary Bypass ◽  
Myocardial Protection ◽  
Septal Defect ◽  
Troponin I ◽  
Control Group ◽  
Conditioning Group ◽  
Cardioplegic Arrest ◽  
Creatine Kinase Mb

AbstractBackgroundRemote ischaemic pre-conditioning and cardiac ischaemic post-conditioning provide myocardial protection in cardiac surgery. However, these two endogenous strategies have not been directly compared in a clinical setting. The purpose of this study was to compare the efficacy of remote ischaemic pre-conditioning and post-conditioning in providing myocardial protection to children undergoing cardiopulmonary bypass for surgical repair of ventricular septal defect.MethodsWe randomly assigned 60 paediatric patients scheduled for surgical correction of congenital ventricular septal defect to the post-conditioning group (n = 20), remote pre-conditioning group (n = 20), or control group (n = 20). Post-conditioning consisted of 30 seconds of ischaemia and 30 seconds of reperfusion achieved by clamping and unclamping the aorta, repeated three times over 3 minutes immediately after cardioplegic arrest. Remote ischaemic pre-conditioning consisted of 5 minutes of lower limb ischaemia followed by 5 minutes of reperfusion using a blood-pressure cuff inflated to a pressure of 200 millimetres of mercury, also repeated three times over 30 minutes. We assayed creatine kinase-MB, troponin I.ResultsMean age, cardiopulmonary bypass times, and aortic cross-clamp times were matched across groups. Both post-conditioning and remote ischaemic pre-conditioning reduced the peak release of creatine kinase-MB (86.1 plus or minus 24.1 units per litre and 92.8 plus or minus 20.6 units per litre, respectively, versus 111.0 plus or minus 44.6 units per litre in the control, p less than 0.05) and troponin I (0.28 plus or minus 0.10 nanogram per millilitre and 0.26 plus or minus 0.09 nanogram per millilitre, respectively, versus 0.49 plus or minus 0.19 nanogram per millilitre in the control group, p less than 0.05).ConclusionsOur study demonstrates that ischaemic post-conditioning and remote ischaemic pre-conditioning provide comparable myocardial benefit in children undergoing cold blood cardioplegic arrest.

scholarly journals Evaluation of anthelmintic efficacy of triclabendazole, nitroxynil and albendazole against naturally acquired fascioliasis in cattle of Bangladesh with special reference to its residual effect

2015 ◽  
Vol 3 (1) ◽  
pp. 1
Author(s):  
Md. Aktaruzzaman ◽  
Zahurin Mohamed ◽  
Naim -Ul-Alam ◽  
Md. Siddiqu Islam ◽  
Md. Matiar Rahman Howlader
Keyword(s):  
Body Weight ◽  
Management Practices ◽  
Residual Effect ◽  
The Body ◽  
Current Status ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Blood Samples ◽  
Group A ◽  
Group D

<p>This study aimed to evaluate the efficacy of triclabendazole, nitroxynil and albendazole against fascioliasis in naturally infected cattle of government dairy farm, Sylhet, Bangladesh. The study included 50 cattle breed of which 30 were naturally infected and randomly selected 20 based on their weight and egg count. Twenty cattle of 2-3 years old irrespective of sex infested with fascioliasis were selected for this experiment and randomly divided into four equal groups (group A, B, C and D) where each group consisted of 5 cattle and cattle of group D were kept as control group. One injectable nitroxynil (10 mgkg-1 body weight, S/C) preparations (Renata Ltd. Bangladesh) and two solid triclabendazole, albendazole (12 mgkg-1, 15 mgkg-1 body weight, orally) preparations (Novartis Ltd. and Square Pharmaceuticals Ltd. Bangladesh) were used for positive control of fascioliasis as group A, B and C. Cattle of group D was kept as control without giving any treatment. Before trials (day 0), total egg count, blood samples and initial body weight were recorded. During the study period the fecal and blood samples were collected directly from rectum and examined on 7<sup>th</sup>, 14<sup>th</sup>, 21<sup>st</sup> and 28<sup>th</sup> day using McMaster fecal egg counting method. Body weight was recorded on day 28 following the treatments. The therapeutic efficacy was evaluated through determination of parasitic prevalence, body weight gain/loss and hematological findings. Pre and post-treatment EPG (eggs per gram) values were recorded, and efficacies compared. The results showed that the efficacy of nitroxynil was 92.57%, followed by triclabendazole 91.55% and albendazole 84.53%, which were significant (p&lt;0.01). The observed differences in efficacy between these three brands of anthelmintics were most likely due to variations either in quality or the administered doses. The body weight of the treated animals was increased, which were significant (p&lt;0.01). After treatment triclabendazole, nitroxynil and albendazole, Total Erythrocyte Count (TEC), Hemoglobin (Hb) content and Packed Cell Volume (PCV) were increased significantly (p&lt;0.01 and p&gt;0.05) in cattle but Erythrocyte Sedimentation Rate (ESR) and Total Leukocyte Count (TLC) were decreased significantly (p&lt;0.05 and p&gt;0.01) in all treated cattle and body weight was increased significantly (p&lt;0.01) on day 28.  The farm management practices along with results of the present study revealed the efficacy of multiple anthelmintics against fascioliasis in cattle. Additional detailed studies are required to clarify the current status of the efficacy of the anthelmintics widely used in different agro ecologies, animal species, and livestock management systems in Bangladesh.</p>

scholarly journals Therapeutic efficacy of ivermectin, fenbendazole and albendazole against naturally occuring gastrointestinal nematodiasis infection in Black Bengal Goat of Bangladesh

2015 ◽  
Vol 3 (1) ◽  
pp. 42 ◽  
Author(s):  
Md. Aktaruzzaman ◽  
Md. Mirazul Islam ◽  
Zahurin Mohamed ◽  
Md. Siddiqul Islam ◽  
Md. Matiar Rahman Howlader
Keyword(s):  
Body Weight ◽  
Therapeutic Efficacy ◽  
Gastrointestinal Nematodes ◽  
The Body ◽  
Current Status ◽  
Total Leukocyte Count ◽  
Control Group ◽  
Blood Samples ◽  
Group A ◽  
Group D

<p>This study aimed to evaluate the efficacy of ivermectin, fenbendazole and albendazole against gastrointestinal nematodes in naturally infected goats of government goat development farm, Sylhet, Bangladesh. The study included 50 black Bengal breed of which 30 were naturally infected and randomly selected 20 on the basis of their weight and egg count. Twenty black Bengal goats of 13-15 month old irrespective of sex infested with gastrointestinal nematodes were selected for this experiment and randomly divided into four equal groups (group A, B, C and D) where each group consisted of 5 goats and goats of group D were kept as control group. One injectable ivermectin (200 µgkg-1 body weight, S/C) preparations (Techno Drugs, Limited, Bangladesh) and two solid fenbendazole, albendazole (7.5 mgkg-1 body weight, orally) preparations (Techno Drugs, Limited and Square Pharmaceuticals Ltd, Bangladesh) were used for positive control of gastrointestinal nematodes as group A, B and C. Goats of group D was kept as control without giving any treatment. Before trials (day 0), total egg count, blood samples and initial body weight were recorded. During the study period the fecal and blood samples were collected directly from rectum and examined on 7<sup>th</sup>, 14<sup>th</sup>, 21<sup>st</sup> and 28<sup>th</sup> day using McMaster fecal egg counting method. Body weight was recorded on day 28 following the treatments. The therapeutic efficacy was evaluated through determination of parasitic prevalence, body weight gain/loss and hematological findings. Pre and post-treatment EPG (eggs per gram) values were recorded, and efficacies compared. The results showed that the efficacy of ivermectin was 100%, followed by fenbendazole 95.33% and albendazole 90.11%. The observed differences in efficacy between these three brands of anthelmintics were most likely due to variations either in quality or the administered doses. The body weight of the treated animals was slightly increased, which were significant (p&lt;0.05). After treatment with ivermectin, fenbendazole and albendazole, Total Erythrocyte Count (TEC), Hemoglobin (Hb) content and Packed Cell Volume (PCV) were increased significantly (p&lt;0.01 and p&gt;0.05) in goats but Erythrocyte Sedimentation Rate (ESR) and Total Leukocyte Count (TLC) were decreased significantly (p&lt;0.05 and p&gt;0.01) in all treated goat and body weight was increased significantly (p&lt;0.01) on day 28. The farm management practices along with results of the present study revealed the efficacy of multiple anthelmintics against gastrointestinal nematodiasis in goat. Additional detailed studies are required to clarify the current status of the efficacy of the anthelmintics widely used in different agro ecologies, animal species and livestock management systems in Bangladesh.</p>

scholarly journals Effort of Varying Intensity as a Factor Influencing the Variability of Selected Biochemical Blood Parameters of Jumping Horses

2012 ◽  
Vol 56 (2) ◽  
pp. 225-229 ◽  
Author(s):  
Hanna Bis-Wencel ◽  
Krzysztof Lutnicki ◽  
Agnieszka Zofia Rowicka ◽  
Bożena Nowakowicz-Dębek ◽  
Monika Bryl
Keyword(s):  
Alkaline Phosphatase ◽  
Creatine Kinase ◽  
Muscle Damage ◽  
Muscle Tissue ◽  
Blood Parameters ◽  
Biochemical Properties ◽  
Blood Samples ◽  
Time Points ◽  
The Difference ◽  
Intensity Characteristic

Abstract Blood samples were collected from horses in four time points of the spring-winter training season, according to the difference in effort intensity characteristic for each period. Serum contents of glucose, cholesterol, and creatinine, as well as activity of creatine kinase, aspartate and alanine aminotransferases, and alkaline phosphatase were determined. Training did not cause muscle damage, and as a result of training, changes in the characteristics of both, contraction and biochemical properties of muscle tissue took place, which was confirmed by the conducted tests.

scholarly journals THE INVESTIGATION OF DNA DAMAGE IN LYMPHOCYTES BY COMET ASSAY IN CHRONIC VIRAL HEPATITIS B PATIENTS

2017 ◽  
Vol 22 (2) ◽  
pp. 64-68
Author(s):  
A. O Mikhailov ◽  
A. F Popov ◽  
N. S Ivanova ◽  
A. I Simakova
Keyword(s):  
Dna Damage ◽  
Liver Fibrosis ◽  
Hepatitis B ◽  
Comet Assay ◽  
Viral Hepatitis ◽  
Chronic Viral Hepatitis ◽  
The Body ◽  
Control Group ◽  
Blood Samples ◽  
Viral Hepatitis B

The investigation of the degree of the lymphocyte DNA damage in chronic viral hepatitis B (HBV) patients is of interest for several reasons. Firstly, it is possible to judge indirectly about the depth of the pathological process at the level of the whole organism, with bearing in mind features of the pathogenic replication of hepatitis B virus. Secondly, it is possible to give an estimation of the degree of genotoxic impact of the virus on blood cells that plays an essential role in the shaping of the immune response of the body. The study was executed on 50 blood samples from HBV patients, divided in 5 groups on the fibrosis grade according to METAVIR score: F0 (n = 10), F1 (N = 10), F2 (N = 10), F3 (n = 10), F4 (n = 10). The control group was consisted of 43 volunteers matched by the age and gender without concomitant diseases. From blood samples taken at the time of the admission to the hospital lymphocytes were isolated by density gradient on Ficoll-urografin. The degree of DNA damage in lymphocytes was determined by virtue of alkaline version of the DNA comet assay. There was noted the direct relationship between an increase in % DNA in the tail of comets and the grade of liver fibrosis. So in the control group, % DNA in the tail accounted for 3.75 ± 1.44. In the F0 group % of DNA in the tail was 5.07 ± 1.25, F1 - 6.79 ± 1.79, F2 - 7.65 ± 1.62, F3 - 8.05 ± 1.18, F4 - 9.84 ± 3.09. It is noteworthy that in groups F2, F3, F4 differences were statistically significant in comparison with the control group. Also there was noted the presence of apoptotic cells in F3, F4 groups: 1 and 0.88%, respectively. Identified changes are both important in the description of to molecular patterns of the pathogenesis of chronic hepatitis B associated with damage, and also can serve as an indirect indication of the stage of liver fibrosis.

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