Donor rehabilitation after repeated blood donations

2020 ◽  
pp. 24-28
Author(s):  
Sergey Pitsul
Keyword(s):  
Oral Administration ◽  
Antibody Titer ◽  
Whole Blood ◽  
High Efficiency ◽  
Recovery Period ◽  
Blood Parameters ◽  
Acute Stage ◽  
Specific Antibodies ◽  
Recovery Processes ◽  
Coronavirus Infection

At the present time the question about using plasma from donors who have had a coronavirus infection and from those with the specific antibodies for transfusion to patients in the acute stage of the process is being repeatedly raised on the background of the worldwide fight against coronavirus. They were the first to resort to such practices in St. Petersburg in Russia, however, it is too early to talk about any high-efficiency transfusions. It is necessary to take into account the fact that in the presence of three hundred patients who need transfusion of immune plasma, an antibody titer has been detected in only 12 donors. Given the existing circumstances, it is necessary to remember about the importance of observing the intervals between donations, whether it is plasma or whole blood. Failure to comply with these intervals, the lack of regular monitoring of clinical and biochemical blood parameters in donors, failure to comply with recommendations for observing the diet and rest between donations can lead to decompensation of recovery processes and seriously affect health of the donor. Donor rehabilitation during the recovery period requires normalization of the diet, and in some cases oral administration of preparations.

scholarly journals In-vitro and in-vivo metabolism of different aspirin formulations studied by a validated liquid chromatography tandem mass spectrometry method

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Michele Dei Cas ◽  
Jessica Rizzo ◽  
Mariangela Scavone ◽  
Eti Femia ◽  
Gian Marco Podda ◽  
...  
Keyword(s):  
Oral Administration ◽  
Whole Blood ◽  
Serum Levels ◽  
Reversed Phase ◽  
Weekly Treatment ◽  
Low Dose Aspirin ◽  
Liquid Chromatography Tandem Mass ◽  
Enteric Coated

AbstractLow-dose aspirin (ASA) is used to prevent cardiovascular events. The most commonly used formulation is enteric-coated ASA (EC-ASA) that may be absorbed more slowly and less efficiently in some patients. To uncover these “non-responders” patients, the availability of proper analytical methods is pivotal in order to study the pharmacodynamics, the pharmacokinetics and the metabolic fate of ASA. We validated a high-throughput, isocratic reversed-phase, negative MRM, LC–MS/MS method useful for measuring circulating ASA and salicylic acid (SA) in blood and plasma. ASA-d4 and SA-d4 were used as internal standards. The method was applied to evaluate: (a) the "in vitro" ASA degradation by esterases in whole blood and plasma, as a function of time and concentration; (b) the "in vivo" kinetics of ASA and SA after 7 days of oral administration of EC-ASA or plain-ASA (100 mg) in healthy volunteers (three men and three women, 37–63 years). Parameters of esterases activity were Vmax 6.5 ± 1.9 and Km 147.5 ± 64.4 in plasma, and Vmax 108.1 ± 20.8 and Km 803.2 ± 170.7 in whole blood. After oral administration of the two formulations, tmax varied between 3 and 6 h for EC-ASA and between 0.5 and 1.0 h for plain-ASA. Higher between-subjects variability was seen after EC-ASA, and one subject had a delayed absorption over eight hours. Plasma AUC was 725.5 (89.8–1222) for EC-ASA, and 823.1(624–1196) ng h/mL (median, 25–75% CI) for plain ASA. After the weekly treatment, serum levels of TxB2 were very low (< 10 ng/mL at 24 h from the drug intake) in all the studied subjects, regardless of the formulation or the tmax. This method proved to be suitable for studies on aspirin responsiveness.

scholarly journals Physiological parameters deterioration in acute stage after stroke: Rehabilitation versus conventional care

2019 ◽  
Vol 10 (3) ◽  
pp. 79
Author(s):  
Reham AbdElhamed AbdElmawla Elsaid ◽  
Amina Mohamed AbdElfatah Sliman
Keyword(s):  
Acute Stroke ◽  
Acute Phase ◽  
Stroke Rehabilitation ◽  
Recovery Period ◽  
Acute Stage ◽  
Conventional Care ◽  
Physiological Parameters ◽  
Stroke Recovery ◽  
University Hospital ◽  
Control Group

Objective: Stroke is considered the main health problem and the second leading cause of death worldwide. Stroke resulting in varied and unpredictable complications if not managed correctly in the acute stage with intensive rehabilitation therapy which may affect stroke prognosis, and resulting functional decline. Therefore, the aim of the study was to explore the consequences of rehabilitation versus conventional care on physiological parameters during the acute stroke recovery period.Methods: The quasi-experimental research design was used in the neurology department at Mansoura University Hospital. A convenient sample of sixty-four adult patients of both sex with stroke, who corresponded to inclusion criteria was assigned into two equal groups, study group (rehabilitation group) and control group (conventional care).Results: The results indicates, acute phase rehabilitation limit physiological parameters deterioration during acute stroke recovery period comparing to conventional care only.Conclusions: Acute phase stroke rehabilitation has a significant positive impact on physiological parameters.

Molecular profile of oral fluid in new coronavirus infection

2021 ◽  
Vol 66 (3) ◽  
pp. 133-138
Author(s):  
F. N. Gilmiyarova ◽  
O. A. Gusyakova ◽  
D. Y. Konstantinov ◽  
I. A. Selezneva ◽  
I. A. Borodina ◽  
...  
Keyword(s):  
Oral Fluid ◽  
Blood Parameters ◽  
The Body ◽  
Molecular Profile ◽  
Gamma Glutamyl Transpeptidase ◽  
Lactate Dehydrogenase Activity ◽  
Invasive Approach ◽  
Calcium Magnesium ◽  
Coronavirus Infection ◽  
Lactate Levels

Oral fluid is an alternative biological material that confirms correlations with blood parameters in various pathological conditions of the body. In order to find a non-invasive approach to stratification of patients with COVID-19 disease, molecular biomarkers of the oral fluid have been determined in patients with moderate coronavirus infection in comparison with clinically healthy individuals. It has been shown that proteomic, carbohydrate, macro- and microelement profiles of the oral fluid in coronavirus infection can be used for diagnostics. The features of protein metabolism were revealed: an increase in the content of total protein, urea; increased activity of enzymes aspartate aminotransferase, gamma glutamyl transpeptidase, creatine phosphokinase, alkaline phosphatase; changes in carbohydrate metabolism, which is expressed by an increase in glucose and lactate levels, an increase in lactate dehydrogenase activity, sodium, chloride, calcium, magnesium, iron content.

Evaluation of the effectiveness of preventive and anti-epidemic measures to prevent the spread of COVID-19 among patients and medical personnel of an antitubercular hospital during the pandemic

2021 ◽  
pp. 8-14
Author(s):  
Temirov Nemat Moidunovich ◽  
Mamyrova Kanykey Kanybekovna ◽  
Abdimomunova Begimay Toktobolotovna ◽  
Satybaldieva Ayzirek Topchubaevna ◽  
Zholdoshev Saparbay Tezekbaevich
Keyword(s):  
High Efficiency ◽  
Medical Personnel ◽  
Hospital Staff ◽  
Protective Equipment ◽  
Tuberculosis Control ◽  
Control Center ◽  
Adequate Ventilation ◽  
Sources Of Infection ◽  
Coronavirus Infection ◽  
Spread Of Infection

The commonality of mechanisms and ways of transmission of new coronavirus infection and tuberculosis increases the risk of spreading pathogens and determines common preventive approaches. The aim of the work was to assess the effectiveness of preventive and anti-epidemic measures to prevent the spread of COVID-19 among patients and medical personnel of an antitubercular hospital during the pandemic. Given the specifics of TB hospital developed and implemented a complex of measures to prevent the spread of COVID-19, which includes the identification and isolation of sources of infection, disinfection of air and adequate ventilation, disinfection of surfaces, use of personal protective equipment. The article describes the group morbidity of COVID-19 patients in an antitubercular hospital, which was caused by the introduction of infection into the hospital by patients in the incubation period. Timely measures made it possible to localize the situation and prevent the widespread spread of infection. The complex of measures for the protection of medical personnel has shown its high efficiency, which was manifested in the absence of cases of illness of personnel working in the «red zone». The absence of a consistent epidemic spread of the new coronavirus infection COVID-19 among patients and hospital staff of the R.G. Bauer Jalal-Abad Regional Tuberculosis Control Center allows us to use the described experience of conducting preventive and anti-epidemic measures in medical organizations of this type.

scholarly journals RESVERATROL INCLUSION COMPLEX WITH β-CYCLODEXTRIN (RCD): CHARACTERIZATION AND EVALUATION OF TOXICITY IN WISTAR RATS

2016 ◽  
Vol 9 (1) ◽  
pp. 27 ◽  
Author(s):  
V. S. K. Nishihira ◽  
N. J. Mezzomo ◽  
M. D. Baldissera ◽  
R. A. Vaucher ◽  
C. G. Pinto ◽  
...  
Keyword(s):  
Inclusion Complex ◽  
Oral Administration ◽  
Biochemical Parameters ◽  
Wistar Rats ◽  
Metabolic Diseases ◽  
Hematological Parameters ◽  
Density Lipoprotein ◽  
Blood Parameters ◽  
Control Group ◽  
Scanning Calorimetry

<p class="RSCB01ARTAbstract"><strong>Objective</strong>:<strong> </strong>The aim of this study was to characterise the resveratrol inclusion complex with β-cyclodextrin (RCD) and evaluate their toxicity in wistar rats.</p><p class="RSCB01ARTAbstract"><strong>Methods: </strong>The RCD were prepared in ultra-turrax. For characterization of the RCD were used: Fourier transform infra-red Spectroscopy, Nuclear Magnetic Resonance (NMR), Differential Scanning Calorimetry (DSC) and X-ray powder diffraction. The RCD and others 4 treatments were performed by the chronic oral administration in 35 rats during 60 ds. After the treatments they were euthanized and the serum blood were collected to analyzed some hemogram and biochemical parameters including aspartyl aminotransferase (AST); alanine aminotransferase (AST); phosphatase alkaline (ALP); total bilirubin (TB); direct bilirubin (DB); total protein (TP); total cholesterol (TC), triacylglycerol (TAG), very low-density lipoprotein (VLDL), high-density lipoprotein (HDL), calcium, iron and phosphate using fully automated biochemistry analyzer.</p><p class="RSCB01ARTAbstract"><strong>Results: </strong>The characterization results indicated a successful formation of the RCD. All hematological parameters analysed were within the normal values in all the groups. Furthermore, the hemogram and biochemical parameters were significantly (P&gt;0.05) similar to the control group.</p><p class="RSCB01ARTAbstract"><strong>Conclusion: </strong>The daily oral administration during 60 d of RCD are not harmful on blood parameters of Wistar rats. Thus, RCD can be used safely for treatment of some metabolic diseases.</p>

scholarly journals The Effect of Laurus nobilis on the Blood and Lenses Antioxidant Activity in Rabbit Under Fat-Enriched Diet

2017 ◽  
pp. 325-333 ◽  
Author(s):  
D. CASAMASSIMA ◽  
F. CHIOSI ◽  
F. VIZZARRI ◽  
M. PALAZZO ◽  
C. COSTAGLIOLA
Keyword(s):  
Antioxidant Activity ◽  
Oral Administration ◽  
Thiobarbituric Acid ◽  
Blood Parameters ◽  
Reactive Oxygen Metabolites ◽  
Laurus Nobilis ◽  
Antioxidant Power ◽  
Total Antioxidant ◽  
Cataract Development ◽  
Plasma Retinol

Fat-enriched diet is strongly associated with cataract development. Laurus nobilis shows antioxidant activity. Herein we evaluated the effect of Laurus nobilis oral administration on the blood and lenses antioxidant activity in rabbits under fat-enriched diet. Sixty rabbits divided into 4 groups were used. One group represented the control (N-CTR). The second group (P-CTR) fed a diet supplemented with 2.5 % of pig fat; the third group (EXP1) received a diet supplemented with 2.5 % of pig fat and 1 g/kg of dried-bay leaves; the fourth group (EXP2) was treated with dried-bay leaves at the rate of 1 g/kg of feed. At baseline and at the end of the study (56 days) the following blood parameters were determined: thiobarbituric acid reactive substances (TBARS), reactive oxygen metabolites (ROMs), total phenols, superoxide dismutase (SOD), oxygen radical absorbance capacity (ORACpca), ferric ion reducing antioxidant power (FRAP), retinol and alfa-tocopherol. At the end of the follow-up, the eyes were enucleated and the antioxidant profile, such as total antioxidant activity (TAC), TBARS, retinol and alfa-tocopherol of lenses was evaluated. Plasma ROMs and TBARS levels were statistically lower in the groups receiving bay leaves integration. A significant increase of plasma retinol, FRAP and ORACpca levels was found in EXP1 and EXP2 groups, whereas plasma alfa-tocopherol resulted statistically higher only in EXP2 group. Bay leaves supplementation enhanced TAC, retinol and alfa-tocopherol in rabbit lens, particularly in EXP2 group; whereas lenses TBARS levels significantly decreased in both treated groups. These findings demonstrate that Laurus nobilis oral administration exerts a protective effect on the risk of cataract development in rabbits under fat-enriched diet.

scholarly journals The dynamics of recovery processes of physical activity of people who recovered from COVID-19

2021 ◽  
Vol 7 (3C) ◽  
pp. 607-612
Author(s):  
Sergey Kokhan ◽  
Elena Romanova ◽  
Vladislav Dychko ◽  
Elena Dychko ◽  
Danil Dychko ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Physical Activity ◽  
Life Assessment ◽  
Control Group ◽  
Breathing Exercises ◽  
Rehabilitation Programs ◽  
Recovery Processes ◽  
Coronavirus Infection ◽  
The Impact

The article shows the results of physical therapy of those who have had coronavirus infection COVID-19 and who have completed a month's course of physical rehabilitation as a part of rehabilitation measures in the conditions of the innovative clinic "Academy of Health". The aim of the research is to study the impact of pulmonary rehabilitation programs for patients with moderate to severe COVID-19. The implementation of special breathing exercises made it possible to improve the oxygen saturation in the blood, to reduce shortness of breath and indicators of respiratory symptoms.  The tolerance to exercise has increased; physical activity and the quality of life of patients with pneumonia associated with COVID-19 have improved. The best results of the EQ-5D quality of life assessment were recorded in the experimental group compared to the control group.

scholarly journals Fast sorting of CD4+ T cells from whole blood using glass microbubbles

TECHNOLOGY ◽  
2015 ◽  
Vol 03 (01) ◽  
pp. 38-44 ◽  
Author(s):  
Chia-Hsien Hsu ◽  
Chihchen Chen ◽  
Daniel Irimia ◽  
Mehmet Toner
Keyword(s):  
T Cells ◽  
T Lymphocyte ◽  
Whole Blood ◽  
Specific Binding ◽  
Target Cells ◽  
Cd4 Cells ◽  
Specific Antibodies ◽  
Resource Limited ◽  
Aids Diagnosis ◽  
Better Than

The isolation of CD4 positive T lymphocyte (CD4+) from peripheral blood is important for monitoring patients after HIV infection. Here, we demonstrate a fast isolation strategy for CD4+ cells that involves mixing blood and glass microbubbles. After the specific binding of target cells to the microbubbles carrying specific antibodies on their surface, target cells will spontaneously float to the top of the blood vial and can be quickly separated. Using this strategy, we demonstrate that the isolation of CD4+ cells in less than 5 minutes and with better than 90% efficiency. This strategy for cell isolation based on buoyancy and glass microbubbles is quick and inexpensive, minimizes blood handling, does not require magnetic fields, or centrifugation equipment, and could lead to new, efficient strategies for AIDS diagnosis in resource-limited areas.

In Vivo Analysis of Gas Transport in Arterial and Venous Blood of the Sea Lamprey Petromyzon Marinus

1992 ◽  
Vol 169 (1) ◽  
pp. 105-119
Author(s):  
B. L. TUFTS ◽  
B. BAGATTO ◽  
B. CAMERON
Keyword(s):  
Partial Pressure ◽  
Whole Blood ◽  
Venous Blood ◽  
Recovery Period ◽  
Co2 Concentration ◽  
Extracellular Acidosis ◽  
Arterial Blood ◽  
Sea Lampreys ◽  
In Vivo Analysis

Exercise in sea lampreys resulted in a significant decrease in the extracellular pH (pHe) in both arterial and venous blood. At rest, the erythrocyte pH (pHi) of venous blood was significantly greater than the pHi of arterial blood. Despite the considerable extracellular acidosis after exercise, both arterial and venous pHi were maintained throughout the recovery period. In the venous blood, there was a reversal of the pH gradient (ΔpH) across the erythrocyte membrane immediately after exercise. Exercise also resulted in significant reductions in the partial pressure of oxygen and hemoglobin oxygen-carriage and a significant increase in the partial pressure of CO2 in arterial and venous blood. Although the total CO2 concentration of the plasma decreased after exercise, erythrocyte total CO2 concentrations (CCOCO2,i) increased. In venous blood, the CCOCO2,i immediately after exercise was double the resting value. At rest, partitioning of the total CO2 content between plasma and erythrocytes indicated that 16 % and 22 % of the total CO2 could be attributed to the erythrocytes in arterial and venous whole blood, respectively. After exercise, these percentages increased to 25% (arterial) and 38% (venous). Changes in CCOCO2,i accounted for 62% of the arteriovenous difference in whole-blood total CO2 at rest. This increased to 78% immediately after exercise. Thus, unlike other vertebrates, CO2 transport in the lamprey in vivo is largely dependent on erythrocyte CO2-carriage.

ERRATA

PEDIATRICS ◽  
1959 ◽  
Vol 23 (5) ◽  
pp. 836-836
Keyword(s):  
Oral Administration ◽  
Acute Stage ◽  
Metabolic Disturbance

Pediatrics, Volume 23, page 585, March, 1959: In the article by Byers, page 597, column 2, line 13, in the sentence, "Oral administration of edathamil is therefore important to shorten the metabolic disturbance due to lead following treatment in the acute stage, even when protection from lead appears to be complete," "important" should read "impotent."

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