scholarly journals UJI EFEK ANTIINFLAMASI KOMBINASI EKSTRAK ETANOL DAUN GYMNANTHEMUM AMYGDALINUM DEL. DAN ELAEIS GUINEENSIS JACQ.

2020 ◽  
Vol 3 (1) ◽  
pp. 1-5
Author(s):  
Wahyudi Wahyudi ◽  
Masnawati Masnawati ◽  
Ismayuni . ◽  
Sofia Rahmi ◽  
Syukur Berkat Waruwu
Keyword(s):  
Palm Oil ◽  
Elaeis Guineensis ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Effective Dosage ◽  
Treatment Groups ◽  
Elaeis Guineensis Jacq ◽  
Anti Inflammatory

Inflammation is a response from the body to injury or infection, flavonoids contained in African leaves (Gymnanthemum Amygdalinum Del.) And palm oil leaves (Elaeis Guineensis Jacq.) have anti-inflammatory effects. This study aims to examine the anti-inflammatory effects of a combination of ethanol extracts of Gymnanthemum Amygdalinum Del. And Elaeis Guineensis Jacq. on white rat foot edema which is induced by carrageenan 1% subplantar, and effective dosage as anti-inflammatory. The extract was made by maceration method using 96% ethanol solvent. The antiinflammatory effect test was divided into 5 treatment groups. The first group (negative control) was given 0.5% CMC Na suspension, the group (positive control) was given diclofenac Na suspension 2.25 mg / kg bw, and the suspension group was a combination of African leaf extract (Gymnanthemum Amygdalinum Del.) And palm oil leaves ( Elaeis Guineensis Jacq.) Dose 200 + 62.5 mg / kg bw, 400 + 125 mg / kg bw, and 800 + 250 mg / kg bw. Inflammation volume measurements were measured using a pletismometer for 180 minutes at 30 minute intervals after 1% carrageenan was induced. The results showed that negative controls had significant differences with other treatment groups which did not show anti-inflammatory effects. In conclusion, the suspension of a combination of ethanol extract of African leaves and palm oil leaves has an effective anti-inflammatory effect at a dose of 800 + 250 mg / kg bw.

scholarly journals PERBANDINGAN EFEK PENYEMBUHAN LUKA BAKAR ANTARA GEL EKSTRAK ETANOL DAUN AFRIKA (Gymnanthemum amygdalinum Del.)DENGAN GEL EKSTRAK ETANOL DAUN KELAPA SAWIT ( Elaeis guineensis Jacq.) SERTA KOMBINASINYA PADA KELINCI TAHUN 2019

2020 ◽  
Vol 3 (1) ◽  
pp. 82-90
Author(s):  
Suventi Syafrina Ginting
Keyword(s):  
Elaeis Guineensis ◽  
Test Preparation ◽  
Ethanol Extract ◽  
Positive Control ◽  
Fourth Group ◽  
Treatment Groups ◽  
Extract Concentration ◽  
Elaeis Guineensis Jacq ◽  
Negative Controls ◽  
Palm Leaf

African leaves (Gymnanthemum amygdalinum Del.) And palm leaves (Elaeis guineensis Jacq.) Have secondary metabolites, namely alkaloids, steroids / terpenoids, flavonoids, tannins, and saponins in this study aimed to determine the effectiveness of healing burns. The extract was made by maceration method using 96% ethanol. This test used 3 rabbits, each of which had been burnt into 6 wound areas on the back of the rabbit. First group (positive control), second group (without treatment), third group gel with Arfika leaf ethanol extract concentration of 5%, fourth group gel with palm ethanol extract concentration of 7.5%, fifth group of groups gel with a combination of Arfika leaf ethanol extract concentration of 5% and 7.5% palm oil leaf ethanol extract The burns that have been made are smeared with as much test preparation as possible once a day. The next day the diameter of the wound was measured, then smeared with an adequate amount of gel. This is done until the diameter of the wound is equal to zero or the wound has been covered by new tissue. The results of testing the effectiveness of healing burns showed that all treatment groups had a faster recovery day compared to negative controls. The conclusion of this study is the combination of 5% arfika ethanol extract gel and 7.5% oil palm leaf ethanol extract is the group with the fastest fastest day on the 19th day.

scholarly journals PENGUJIAN EFEKTIVITAS DIURETIK KOMBINASI EKSTRAK ETANOL DAUN AFRIKA (Gymnanthemum amygdalinum Del.) DAN DAUN KELAPA SAWIT (Elaeis guineensis Jacq.) PADA TIKUS JANTAN

2020 ◽  
Vol 3 (1) ◽  
pp. 106-111
Author(s):  
Delisma Simorangkir
Keyword(s):  
Leaf Extract ◽  
Elaeis Guineensis ◽  
Urine Volume ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Treatment Groups ◽  
White Rats ◽  
Group 2 ◽  
Palm Leaf

African Leaf and palm leaf crops have a secondary metabolite content such as alkaloids and flavonoids that are efficacious as diuretics. The purpose of this study is to know the effectiveness of diuretics from the combination  African leaf ethanol extract and palm leaf ethanol extract. Methods used Eksperiemental. The test of diuretic activity is conducted divided into 6 treatment groups. Each group consists of 3 tails of white rats. Group 1 (positive control) administered Furosemide 3.6 mg/kgBB, Group 2 (negative control) was given suspension Na-CMC 0.5%, group 3 without treatment as well as groups of 4.5, and 6 in a row administered a combination of African leaf extract doses and extracts Oil palm leaf ethanol in a row of "50 mg/kgBB + 67, 5mg/kgBB"; "100mg/kgBB + 135 mg/kgBB"; and "200 mg/kgBB + 270 mg/kgBB". Each mouse is then given a 20ml NaCl/kgBB as an oral loading dose. Measured urine volume is recorded every hour for 6 hours after it continues to measure urine volume. The results showed that the combination of African leaf ethanol extract dosage and palm leaf ethanol extract had a diuretic effect if it was compared with negative control. The conclusion of the study was the results of the study showed that the combination of African leaf extract dosage and palm leaf extract is the most effective dose of Group 6 (African leaf extract 200 mg/kgBB + palm leaves 270 mg/kgBB ). Because it produces the highest urine volume of 23.01 ml.

scholarly journals AKTIVITAS ANTI-INFLAMASI EKSTRAK ETANOL TEPUNG PELEPAH AREN (Arenga pinnanta)

2020 ◽  
Vol 13 (2) ◽  
Author(s):  
Devy Angreani M ◽  
Meiske Sangi ◽  
Feti Fatimah
Keyword(s):  
Ethanol Extract ◽  
Negative Control Group ◽  
Positive Control ◽  
Negative Control ◽  
Inflammatory Activity ◽  
Control Group ◽  
Treatment Groups ◽  
White Rats ◽  
Wistar Strain ◽  
Anti Inflammatory

Tepung pelepah aren secara tradisional digunakan sebagai obat untuk menghilangkan rasa gatal dan luka bakar pada kulit. Penelitian ini bertujuan untuk menguji aktivitas anti-inflamasi ekstrak etanol tepung pelepah aren (Arenga pinnanta) menggunakan metode induksi karagenan. Ekstrak etanol tepung pelepah aren yang digunakan didapatkan dari hasil maserasi tepung pelepah aren menggunakan etanol hasil redestilasi cap tikus. Metode pengujian aktivitas anti-inflamasi menggunakan 15 ekor tikus putih jantan galur wistar dengan berat 150-200 gram yang dibagi dalam 5 kelompok perlakuan, Kelompok Kontrol negative, Kontrol Positif dan kelompok dosis ekstrak etanol 20%, 25% dan 30%. Hasil penelitian ini menunjukkan kelompok ekstrak etanol tepung pelepah aren dosis 30% memiliki kemampuan inhibisi udem yang sama dengan kontrol positif (Na Diklofenak) dan memiliki kemampuan inhibisi yang lebih besar dibandingkan dosis 20% dan 25%.ABSTRACTPalm stem flour is traditionally used as a remedy for the relief of itching and burns on the skin. This research aims to test the anti-inflammatory activity of palm-flour (Arenga pinnanta) ethanol extract using Caragenan induction method. Palm-dried stem flour extract used from the maceration of palm flour, using ethanol redestilation of rat seals. The method of testing anti-inflammatory activity using 15 male white rats with Wistar strain with a weight of 150-200 grams divided into 5 treatment groups, negative control group, positive control and group dose of ethanol extract 20%, 25% and 30%. The results of this study showed that the group of dried palm flour extract dose 30% have the same inhibition capability with positive control (Na Diklofenak) and have greater inhibition capability compared to the dose 20% and 25%.

UJI EFEK ANALGESIK EKSTRAK ETANOL DAUN KERSEN (Muntingia calabura L.) PADA MENCIT PUTIH JANTAN (Mus musculus) DENGAN INDUKSI NYERI ASAM ASETAT

2017 ◽  
Vol 2 (2) ◽  
pp. 147
Author(s):  
Triswanto Sentat ◽  
Susiyanto Pangestu
Keyword(s):  
Analgesic Effect ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Control Treatment ◽  
Treatment Groups ◽  
Analgesic Effects ◽  
Significant Difference ◽  
Analgesic Dose ◽  
Muntingia Calabura

Kersen leaf (Muntingia calabura L.) contains tannins, flavonoids and polyphenol compounds allegedly have analgesic effect. The objective was to determine the analgesic effect of ethanol extract of kersen leaves and to determine the most effective analgesic dose. This study was an experimental research. Leaves were extracted with ethanol 70% and the analgesic effect test was divided into 5 groups: negative control treatment (distilled water), positive control (mefenamic acid 2.6mg/kg), kersen leaf ethanol extract first dose (100mg/kg), second dose (200mg/kg) and tthird dose (400mg/kg). Giving treatments by oral, after 30 minutes, the mices were given a pain inductor with 0.5% acetic acid by intra peritonial administration. Analgesic power was calculated by counting the number of writhing in mice for 1 hour. The results showed that the ethanol extract of cherry leaf has analgesic effect. From the calculation of the first dose analgesic power (42.9%), second dose (59.4%) and the third dose 69.9%. Statistical test results kruskal wallis value of p=0.011 (p<0.05) showed a significant difference between all analgesic treatment groups. The conclusion of this study is all of the ethanol extract had analgesic effects on male white mice, whereas a dose of 400mg/kg is the most effective analgesic dose.

scholarly journals EFEKTIVITAS SALEP KOMBINASI EKSTRAK DAUN BANGUN – BANGUN (Coleus amboinicus Lour.) DAN DAUN KELAPA SAWIT (Elaeis guineensis Jacq.) SEBAGAI OBAT LUKA SAYAT

2020 ◽  
Vol 3 (1) ◽  
pp. 48-56
Author(s):  
Rika Puspita Sari
Keyword(s):  
Wound Healing ◽  
Single Dose ◽  
Elaeis Guineensis ◽  
Ethanol Extract ◽  
Rabbit Skin ◽  
Treatment Groups ◽  
Healing Wound ◽  
Elaeis Guineensis Jacq ◽  
Coleus Amboinicus ◽  
All Treatment

Bangun-bangun leaves and palm leaves contain various secondary metabolites such as tannins, alkaloids and flavonoids which can healing wound. The aim of this study was to determine the effect of ointment combination of ethanol extract of leaves of bangun- bangun (Coleus amboinicus Lour.) And ethanol extract of palm leaves (Elaeis guineensis Jacq.) In healing wound. The bangun- bangun leaves and the palm leaves are separated from the petiole and then dried in a drying cupboard so that it becomes a simplisia. Simplisia is extracted by maceration method then evaporated with a rotary evaporator and evaporated again on a water bath to produce a thick extract. Thick extracts from the leaves of bangun- bangun and palm leaves are then formulated into ointment preparations with varying concentrations. Each rabbit was shaved on its back then cleaned with 70% alcohol. Furthermore, rabbits were anesthetized using 0.5 ml Lidocain HCL as much as 0.5 ml subcutaneously. Next mark the part that will be injured with a diameter of 2 cm, by lifting the rabbit skin using tweezers and then made a wound using surgical scissors that have been sterilized first with 70% alcohol. Wound diameter measurements showed that all treatment groups from day 1 to day 23 experienced changes in wound diameter. The combination ointment of ethanol extract of leaves wake-up (EEDB) 10% and ethanol extract of palm oil leaves (EEDKS) 10% have a more effective effect in wound healing than single dose.

scholarly journals Evaluation the Effect of Vernonia amygdalina. Del Leaves Ethanol Extract on Blood Sugar and its Synergism with Glibenclamide in Rats

2020 ◽  
Vol 13 (1) ◽  
pp. 26-34
Author(s):  
Igwe K ◽  
Ikpeazu O ◽  
Otuokere I
Keyword(s):  
Blood Glucose ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Diabetic Rats ◽  
Antidiabetic Activity ◽  
Control Group ◽  
Vernonia Amygdalina ◽  
Treatment Groups ◽  
Glucose Levels

Antidiabetic activity of Vernonia amygdalina and its possible synergism with glibenclamide was checked. Forty eight rats were used for the research, for hypoglycermic study of V. amygdalina alone, they were grouped into five of six rats each. Group 1 was the negative control and was administered distilled water orally. Groups 2, 3, and 4 were the treatment groups which received 100, 200 and 300 mg/kg body weight of the V. amygdalina extract respectively orally by intubation. Group 5 was the positive control group which received a known antidiabetic drug, glibenclamide. Diabetes was induced with alloxan. For the synergism study, another 18 rats grouped into 3 of six rats each was used. Both groups of glibenclamide only and glibenclamide plus V. amygdalina extract were dosed for 14 days orally by intubation, thereafter were sacrificed and blood collected from heart for analysis. There were 5 replicates grouped by weight throughout the study and both single and synergistic studies had the same controls. Effect of V. amygdalina extract was checked on blood glucose and its possible synergism with glibenclamide. All results in treatment groups were compared with the normal control at statistical confidence of p<0.05. Result shows that V. amygdalina extract reduced blood glucose level in the test groups as dose of extract increased. Combination of V. amygdalina with glibenclamide demonstrated further deduction in blood glucose levels in the treatment rats groups. Therefore addition of V. amygdalina into glibenclamide increased efficacy in the diabetic rats. The interaction between V. amygdalina and glibenclamide in this work was additive and therefore synergistic.

scholarly journals AKTIVITAS ANTIFUNGI ASAP CAIR DARI TANDAN KOSONG Elaeis guineensis Jacq.TERHADAP Colletotrichum sp. (WA2)

2020 ◽  
Vol 7 (2) ◽  
pp. 271-279
Author(s):  
Elvi Rusmiyanto Pancaning Wardoyo ◽  
Widya Anggraeni ◽  
. Rahmawati ◽  
Hasan Ashari Oramahi
Keyword(s):  
Oil Palm ◽  
Elaeis Guineensis ◽  
Positive Control ◽  
Negative Control ◽  
Potato Dextrose Agar ◽  
Activity Level ◽  
Capsicum Frutescens ◽  
Liquid Smoke ◽  
Wood Vinegar ◽  
Elaeis Guineensis Jacq

Antifungal Activity of Wood Vinegar derived from Oil Palm Empty Bunches against Colletotrichum sp. (WA2) Colletotrichum sp. is a fungus that causes anthracnose in cayenne pepper (Capsicum frutescens L.).  An alternative natural control for this fungus is using wood vinegar. The aim of this study was to evaluate antifungal properties of wood vinegar from oil palm empty bunches (Elaeis guineensis Jacq.) against Colletotrichum sp. The antifungal test was carried out using the solid dilution method by poisoning food in potato dextrose agar (PDA) media. Colletotrichum sp. used was isolated from red chili which had anthracnose symptoms. This experimental research method used a completely randomized design (CRD) with 9 treatments, namely, negative control (non-liquid smoke), positive control (Dithane M45 at 0.20%), liquid smoke concentration of 0.40; 0.42; 0.44; 0.46; 0.48; 0.50; and 0.52%. Anova test results showed wood vinegar from empty fruit bunches of E. guineensis at concentrations of 0.40; 0.42; 0.44; 0.46 and 0.48% differed significantly from negative control, positive control, and concentrations of 0.50 and 0.52%. The concentration of 0.42% was the minimum inhibitory concentration with an average value of 87.98% inhibition and a very strong activity level. In conclusion, the wood vinegar from E. guineensis can be used to control Colletotrichum sp fungus at an effective concentration of 0.42%. Colletotrichum sp. merupakan jamur penyebab penyakit antraknosa pada tanaman cabai rawit (Capsicum frutescens L.). Salah satu alternatif pengendalian secara alami terhadap jamur tersebut adalah dengan menggunakan asap cair. Penelitian ini bertujuan untuk mengevaluasi sifat antijamur asap cair dari tandan kosong Elaeis guineensis Jacq (kelapa sawit) terhadap Colletotrichum sp. Uji antijamur dilakukan dengan metode dilusi padat melalui cara poisoning food dalam media potato dextrose agar (PDA). Isolat jamur Colletotrichum sp. yang digunakan merupakan hasil isolasi dari tanaman cabe merah yang bergejala antraknosa. Metode penelitian ini mengunakan rancangan acak lengkap (RAL) dengan 9 perlakuan, yaitu kontrol negatif (tanpa asap cair), kontrol positif (Dithane M45 sebesar 0,20%), konsentrasi asap cair sebesar 0,40; 0,42; 0,44; 0,46; 0,48; 0,50; dan 0,52%. Hasil uji Anova menunjukkan asap cair dari tandan kosong E. guineensis pada konsentrasi 0,40; 0,42; 0,44; 0,46; dan 0,48% berbeda secara signifikan dengan kontrol negatif, kontrol positif dan konsentrasi 0,50 dan 0,52%. Konsentrasi 0,42% merupakan konsentrasi hambat minimum dengan nilai rata-rata penghambatan sebesar 87,98% dan tingkat aktivitas sangat kuat. Dengan demikian dapat disimpulkan bahwa asap cair dari tandan kosong E. guineensis dapat digunakan untuk mengendalikan jamur Colletotrichum sp. pada konsentrasi efektif 0,42%.

scholarly journals HEPATOPROTECTIVE EFFECT OF A 70% ETHANOL EXTRACT OF GOD'S CROWN FRUIT (PHALERIA MACROCARPA) AGAINST PARACETAMOL HEPATOTOXICITY IN SWISS WEBSTER MICE

2019 ◽  
Vol 4 (1) ◽  
pp. 18
Author(s):  
Tejo Jayadi
Keyword(s):  
Hepatic Injury ◽  
Ethanol Extract ◽  
Positive Control ◽  
Control Treatment ◽  
Control Group ◽  
Toxic Metabolite ◽  
Toxic Dose ◽  
Treatment Groups ◽  
Phaleria Macrocarpa ◽  
Anti Inflammatory

Background: The god’s crown fruits have properties as antioxidants and anti-inflammatory. Toxic doses of paracetamol can injure the liver through toxic metabolite bonds with cytoplasmic proteins that cause free radicals to form. The aim of this research is to know the effect of the crown of gods extracts on paracetamol hepatotoxicity. Method: A total of 30 of Webster swiss mice with a weight of ± 20 grams, age 3 months were randomly assigned to five groups, negative control, positive control, treatment 1,2 and 3. A 70% ethanol extract of god’s crown fruit given in doses 60mg, 120mg and 240mg per kgBB mice. The extract was administered for 14 days in the treatment groups, then on day 15 paracetamol ware administered in a given dose 300mg/kgBB for 1 day for the positive control group and treatment groups. On day 16, the serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels were examined from the orbital sinuses and animals terminated liver tissues taken and immediately fixed in 10% buffer formalin for histological examination. Results and Discussion: The 70% ethanol extract of the god’s crown fruits decreased blood serum levels of AST and ALT, and these results were supported by histopathologic scores of the liver in which histopathologic scores were improved with the increasing doses (p < 0.05). The secondary metabolite contents of the god’s crown fruit extract served as an antioxidant and anti-inflammatory, protecting hepatic injury from the toxic metabolite of paracetamol. Conclusion: A 70% ethanol extract of god’s crown fruit (Phaleria macrocarpa) have hepatoprotective properties that effectively prevent hepatic injury due to paracetamol toxic dose.

The enhancement of Arg1 and activated ERβ expression in microglia HMC3 by induction of 96% ethanol extract of Marsilea crenata Presl. leaves

2020 ◽  
Vol 30 (6) ◽  
Author(s):  
Burhan Ma’arif ◽  
Mangestuti Agil ◽  
Hening Laswati
Keyword(s):  
Laser Scanning ◽  
Negative Relationship ◽  
Ethanol Extract ◽  
Positive Control ◽  
Negative Control ◽  
Scanning Microscope ◽  
Treatment Groups ◽  
Arginase 1 ◽  
Significant Difference ◽  
Ifn Γ

AbstractBackgroundPhytoestrogens have a high potential to overcome the neuroinflammation caused by estrogen deficiency. Marsilea crenata Presl. is a plant known to contain phytoestrogens. This research aimed to report the activity of a 96% ethanol extract of M. crenata leaves in inducing activation of microglia HMC3 cell to M2 polarity, which has anti-inflammatory characteristics.MethodsThe study was done by culturing microglia HMC3 cell in 24-well microplate and inducing it with IFN-γ for 24 h to activate the cell to M1 polarity, which has proinflammatory characteristics. The 96% ethanol extract was added with various doses of 62.5, 125, and 250 ppm. Genistein, 50 μM, was used as a positive control. The analysis of the immunofluorescence of Arginase-1 (Arg1) and ERβ as markers was done using a convocal laser scanning microscope.ResultsThe result of Arg1 shows a significant difference in Arg1 expression in the microglia HMC3 cell line between the negative control and all treatment groups at p < 0.05, with the best result at 250 ppm, whereas for ERβ, the results show, at doses of 125 and 250 ppm, that the 96% ethanol extract of M. crenata leaves decrease the activated ERβ expression at p < 0.05, with the best result at 250 ppm. The Arg1 and activated ERβ expression have a weak negative relationship with the Pearson correlation test.ConclusionsThe 96% ethanol extract of M. crenata leaves has an antineuroinflammation activity through the induction of Arg1 and activated ERβ expression in microglia HMC3 cell, with the best dose at 250 ppm.

Effect of Topical Noni (Morinda citrifolia L.) Leaf Extract Paste in Carrageenan-induced Paw Edema on Wistar Rats

2019 ◽  
Vol 7 (2) ◽  
Author(s):  
Indah Puti Rahmayani Sabirin ◽  
Euis Reni Yuslianti
Keyword(s):  
Inflammatory Response ◽  
Wistar Rats ◽  
Leaf Extract ◽  
Positive Control ◽  
Negative Control ◽  
The Body ◽  
Morinda Citrifolia ◽  
Paw Edema ◽  
Anti Inflammatory ◽  
Inflammatory Effect

The inflammatory response is one of natural process in the body to protect itself following tissue injury, but it can cause discomfort. Noni (Morinda citrifolia L.) leaf known as a traditional medicament to help reduce the inflammatory effect. The leaves empirically applied as a wrapping on fever or wound. The purpose of this study was to identify the anti-inflammatory effect of topical noni leaf extract paste in 5% and 10% concentration by examination of Wistar rat paw edema induced by λ-carrageenan. Twenty-four Wistar rats divided into four groups, which were negative control, positive control with diclofenac sodium 1% gel, 5% noni leaf, and 10% noni leaf paste groups. Paw edema was induced by intraplantar injection of 1% λ-carrageenan to every rat. Every treatment subsequently applied in the plantar area before injection, and the changed paw volume measured with plethysmometer at minutes 0, 30, 60, and 90. This study was at the Animal Laboratory, Faculty of Medicine, Universitas Jenderal Achmad Yani, Cimahi city in October–December 2017. The result displayed that the minimum volume after 90 minutes was on 5% and 10% noni leaf paste group, which is 1.00 mL. Kruskal-Wallis test result of inflammatory percentage was significantly different among every group in each examination time (p<0.05). Post-hoc test showed that inflammatory reduction on paw edema with noni leaf paste application on both concentrations were significantly different compared to the negative control. However, it was not different from the positive control group. This study showed that application of noni leaf paste in 5% and 10% concentration could help reduce inflammatory response on skin possibly by the active anti-inflammatory ingredients of noni leaf. EFEK PASTA EKSTRAK DAUN MENGKUDU (MORINDA CITRIFOLIA L.) TOPIKAL TERHADAP EDEMA KAKI TIKUS GALUR WISTAR YANG DIINDUKSI KARAGENANInflamasi adalah proses alami tubuh untuk melindunginya setelah cedera, namun hal tersebut dapat menyebabkan ketidaknyamanan. Daun mengkudu (Morinda citrifolia L.) dikenal sebagai obat tradisional untuk menurunkan efek inflamasi yang secara empiris digunakan untuk mengobati demam dan luka. Tujuan penelitian ini adalah mengetahui efek anti-inflamasi pasta ekstrak daun mengkudu melalui pemeriksaan edema kaki tikus yang diinduksi karagenan-λ. Dua puluh empat tikus galur Wistar dibagi menjadi 4 kelompok, yaitu kontrol negatif dengan akuades, kontrol positif (aplikasi gel Na diklofenak 1%), serta perlakuan pasta daun mengkudu 5% dan 10%. Edema dibuat dengan menginjeksi intraplantar tikus dengan 1% karagenan-λ pada tiap kelompok. Tiap-tiap perlakuan diaplikasikan sebelum tikus diinjeksi dan perubahan volume kaki tikus diukur dengan pletismometer di menit ke-0, 30, 60, dan 90. Penelitian ini dilakukan di Laboratorium Hewan, Fakultas Kedokteran, Universitas Jenderal Achmad Yani, Kota Cimahi pada Oktober–Desember 2017. Hasil pengukuran memperlihatkan penurunan volume edema kaki terkecil setelah 90 menit pada kelompok pasta daun mengkudu 10%, yaitu 1,00 mL. Hasil Uji Kruskal-Wallis terhadap persentase inflamasi berbeda nyata pada tiap kelompok dan tiap waktu pengamatan (p<0,05). Hasil uji beda menunjukkan bahwa penurunan inflamasi kaki tikus pada perlakuan pasta daun mengkudu kedua konsentrasi berbeda nyata dibanding dengan kontrol negatif, tetapi tidak berbeda dengan kontrol positif. Penelitian ini menunjukkan bahwa pasta daun mengkudu 5% dan 10% dapat membantu menurunkan reaksi inflamasi kulit dan efeknya sejalan dengan Na diklofenak karena zat aktif yang bersifat anti-inflamasi dalam daun mengkudu.

Sign in / Sign up

Export Citation Format

Share Document